研究者詳細 - 最相　大輔

Analysis of single nucleotide polymorphisms based on RNA sequencing data of diverse bio-geographical accessions in barley

Kotaro Takahagi, Yukiko Uehara-Yamaguchi, Takuhiro Yoshida, Tetsuya Sakurai, Kazuo Shinozaki, Keiichi Mochida, Daisuke Saisho

SCIENTIFIC REPORTS 6 2016年9月

記述言語：英語出版者・発行元：NATURE PUBLISHING GROUP

Barley is one of the founder crops of Old world agriculture and has become the fourth most important cereal worldwide. Information on genome-scale DNA polymorphisms allows elucidating the evolutionary history behind domestication, as well as discovering and isolating useful genes for molecular breeding. Deep transcriptome sequencing enables the exploration of sequence variations in transcribed sequences; such analysis is particularly useful for species with large and complex genomes, such as barley. In this study, we performed RNA sequencing of 20 barley accessions, comprising representatives of several biogeographic regions and a wild ancestor. We identified 38,729 to 79,949 SNPs in the 19 domesticated accessions and 55,403 SNPs in the wild barley and revealed their genome-wide distribution using a reference genome. Genome-scale comparisons among accessions showed a clear differentiation between oriental and occidental barley populations. The results based on population structure analyses provide genome-scale properties of sub-populations grouped to oriental, occidental and marginal groups in barley. Our findings suggest that the oriental population of domesticated barley has genomic variations distinct from those in occidental groups, which might have contributed to barley's domestication.

DOI： 10.1038/srep33199

野生オオムギと栽培オオムギにおける胚乳細胞壁の厚さに関する解析

最相大輔, 松島良, 本庄三恵, 八杉公基, 永野惇, 永野惇, 高萩航太郎, 高萩航太郎, 持田恵一, 持田恵一, 持田恵一, 武田真, 坂本亘

育種学研究 18 2016年

オオムギ発芽時耐塩性のQTL解析

伊藤大樹, 最相大輔, 本庄三恵, 八杉公基, 永野惇, 高萩航太郎, 持田恵一, 佐藤和広

育種学研究 17 236 2015年3月

「コシヒカリ」と「ヒヨクモチ」との交雑に由来するイネ組換え自殖系統群における出穂期および農業形質のQTL解析

前田道弘, 岡田聡史, 合田喬, 佐々木萌, 末廣美紀, 横山若菜, 高山隆一, 最相大輔, 山本洋, 堀清純, GARCIA Arturo, 土井一行, 山崎将紀

育種学研究 17 2015年

バイオリファイナリー利用に向けた稲わらの多様性

合田喬, 寺村浩, 末廣美紀, 高山隆一, 最相大輔, 山本洋, 金丸研吾, 川口秀夫, 荻野千秋, 近藤昭彦, 山崎将紀

育種学研究 17 2015年

栽培オオムギのアルミニウム耐性がもたらす東アジアへの適応分化

最相大輔, 大西一光, 伊藤大樹, 久保寺秀夫, MA J., 佐藤和広

育種学研究 16 2014年

表現形質評価システム「FieldBook」を用いたイネ稈長と穂長の計測

前田道弘, 岡田聡史, 末廣美紀, 合田喬, 伊藤田鶴子, 山本洋, 最相大輔, GARCIA Arturo, 山崎将紀

育種学研究 16 2014年

麦類の遺伝子単離と機能解明,麦類における有用遺伝子の同定・機能解明と品種改良に向けたDNAマーカーの開発 4 オオムギ穂の形態形成遺伝子の単離と機能解明(TRG1004)

小松田隆夫, 掛田克行, 最相大輔, 半田裕一

農林水産省農林水産技術会議事務局研究成果 ( 513 ) 2014年

「FieldBook」を使ったオオムギ遺伝資源の大規模表現型測定

伊藤大樹, 岡田聡史, ARTURO Garcia, 石井誠, 伊藤田鶴子, 山本洋, 山崎将紀, 最相大輔, 佐藤和広

育種学研究 15 2013年

Acquisition of aluminium tolerance by modification of a single gene in barley. 国際誌

Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takahashi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

Nature communications 3 713 - 713 2012年3月

記述言語：英語

Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.

DOI： 10.1038/ncomms1726

11-11 オオムギのアルミニウム耐性機構の獲得(11.植物の有害元素,2012年度鳥取大会)

柏野美帆, 横正健剛, 山地直樹, 最相大輔, 山根美樹, 高橋宏和, 佐藤和広, 中園幹生, 馬建鋒

日本土壌肥料学会講演要旨集 58 ( 0 ) 76 - 76 2012年

オオムギ春播性遺伝子Vrn-H1の近傍に見出された新規日長反応性遺伝子HvPhyC

西田英隆, 金古卓磨, 石原大輔, 川東広幸, 半田裕一, 石井誠, 最相大輔, 明石由香利, 武田和義, 加藤鎌司

育種学研究 14 2012年

オオムギのアルミニウム耐性獲得の進化過程

最相大輔, 藤井美帆, MA J., 佐藤和広

育種学研究 14 2012年

植物ゲノムへ水平伝播された太古のパルティティウイルス配列

千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

日本植物病理学会報 78 ( 1 ) 2012年

栽培オオムギにおける春化要求性の自然変異:高度秋播性は東アジアに偏在する

最相大輔, 石井誠, 堀清純, 佐藤和広

育種学研究 13 80 2011年9月

QTL解析の実践 : 押さえておきたい3つのキーワード「連続分布」「分散」「遺伝率」

最相大輔, 堀清純

種生物学研究 34 51 - 62 2011年7月

Widespread Endogenization of Genome Sequences of Non-Retroviral RNA Viruses into Plant Genomes

Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

PLOS PATHOGENS 7 ( 7 ) e1002146 2011年7月

記述言語：英語出版者・発行元：PUBLIC LIBRARY SCIENCE

Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant-and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single-and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species.

DOI： 10.1371/journal.ppat.1002146

Natural variation of barley vernalization requirements : Implication of quantitative variation of inter growth habit as an adaptive trait in East Asia.

最相大輔

Plant and Cell Physiology 52 ( 5 ) 775 - 84 2011年5月

Barley: Emergence as a New Research Material of Crop Science

Daisuke Saisho, Kazuyoshi Takeda

PLANT AND CELL PHYSIOLOGY 52 ( 5 ) 724 - 727 2011年5月

オオムギ種子の耐水性の地理的分布

最相大輔, 石井誠, 佐藤和宏, 武田和義

育種学研究 13 2011年

非レトロRNAウイルス由来の植物染色体配列

近藤秀樹, 千葉壮太郎, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

日本植物病理学会大会プログラム・講演要旨予稿集 2011 2011年

Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos

Kazuhide Rikiishi, Daisuke Saisho, Kazuyoshi Takeda

Proceedings of the 10th international barley genetics symposium 679 - 686 2010年

非パラレトロRNAウイルス由来の植物染色体遺伝子

千葉壮太郎, 近藤秀樹, 谷明生, 最相大輔, 坂本亘, 兼松聡子, 鈴木信弘

日本ウイルス学会学術集会プログラム・抄録集 58th 2010年

皮性・裸性を支配するNud遺伝子の多型からみた栽培オオムギの起源

武田真, 辻野泰弘, 湯尾崇央, 最相大輔, 漆川直希, 春山直人, 大関美香

育種学研究 12 2010年

六条オオムギの進化過程

最相大輔, MOHAMMAD Pourkheirandish, 小松田隆夫

育種学研究 11 2009年

TriMEDB: A database to integrate transcribed markers and facilitate genetic studies of the tribe Triticeae

Keiichi Mochida, Daisuke Saisho, Takuhiro Yoshida, Tetsuya Sakurai, Kazuo Shinozaki

BMC PLANT BIOLOGY 8 72 2008年6月

記述言語：英語出版者・発行元：BIOMED CENTRAL LTD

Background: The recent rapid accumulation of sequence resources of various crop species ensures an improvement in the genetics approach, including quantitative trait loci (QTL) analysis as well as the holistic population analysis and association mapping of natural variations. Because the tribe Triticeae includes important cereals such as wheat and barley, integration of information on the genetic markers in these crops should effectively accelerate map-based genetic studies on Triticeae species and lead to the discovery of key loci involved in plant productivity, which can contribute to sustainable food production. Therefore, informatics applications and a semantic knowledgebase of genome-wide markers are required for the integration of information on and further development of genetic markers in wheat and barley in order to advance conventional marker-assisted genetic analyses and population genomics of Triticeae species.
Description: The Triticeae mapped expressed sequence tag (EST) database (TriMEDB) provides information, along with various annotations, regarding mapped cDNA markers that are related to barley and their homologues in wheat. The current version of TriMEDB provides map-location data for barley and wheat ESTs that were retrieved from 3 published barley linkage maps (the barley single nucleotide polymorphism database of the Scottish Crop Research Institute, the barley transcript map of Leibniz Institute of Plant Genetics and Crop Plant Research, and HarvEST barley ver. 1.63) and 1 diploid wheat map. These data were imported to CMap to allow the visualization of the map positions of the ESTs and interrelationships of these ESTs with public gene models and representative cDNA sequences. The retrieved cDNA sequences corresponding to each EST marker were assigned to the rice genome to predict an exon-intron structure. Furthermore, to generate a unique set of EST markers in Triticeae plants among the public domain, 3472 markers were assembled to form 2737 unique marker groups as contigs. These contigs were applied for pairwise comparison among linkage maps obtained from different EST map resources.
Conclusion: TriMEDB provides information regarding transcribed genetic markers and functions as a semantic knowledgebase offering an informatics facility for the acceleration of QTL analysis and for population genetics studies of Triticeae.

DOI： 10.1186/1471-2229-8-72

Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos

Kazuhide Rikiishi, Daisuke Saisho, Kazuyoshi Takeda

BREEDING SCIENCE 58 ( 2 ) 149 - 155 2008年6月

記述言語：英語出版者・発行元：JAPANESE SOC BREEDING

Barley includes semi-dwarf varieties, called uzu, which are localized in parts of southwestern Japan, the Southern Korea peninsula, and coastal areas of China. The uzu phenotype possesses dark green leaves and short coleoptiles, awns, and panicles. It is controlled by a single recessive gene: uzu. Uzu results from a mutation in the brassinosteroid receptor kinase gene (HvBR11). Brassinosteroid synergistically acts with auxin on plant morphology, which is an important plant hormone for tissue culture. For this study, tissue Culture traits, including callus growth and shoot regeneration capability, were examined in F-2 populations derived from crosses between normal and uzu lines, and in isogenic lines for the uzu gene. The uzu genotype shows a lower percentage of shoot regeneration than the normal genotype in F, populations and isogenic lines. The uzu gene negatively affects shoot regeneration. No significant differences were Found In callus growth capability between uzu and normal genotypes. Uzu isogenic lines show higher sensitivity to exogenous auxin for callus initiation than normal lines, when immature embryos were incubated on media supplemented with several concentrations of 2,4-D under culture at higher temperature (25 degrees C). Tissue Culture traits of uzu might be regulated through cross-talk between brassinosteroid and auxin.

DOI： 10.1270/jsbbs.58.149

Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway. 国際誌

Shin Taketa, Satoko Amano, Yasuhiro Tsujino, Tomohiko Sato, Daisuke Saisho, Katsuyuki Kakeda, Mika Nomura, Toshisada Suzuki, Takashi Matsumoto, Kazuhiro Sato, Hiroyuki Kanamori, Shinji Kawasaki, Kazuyoshi Takeda

Proceedings of the National Academy of Sciences of the United States of America 105 ( 10 ) 4062 - 7 2008年3月

記述言語：英語

In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (i) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion.

DOI： 10.1073/pnas.0711034105

東アジアに局在するオオムギ半矮性系統”渦”の進化的・形態的・生理的な多様性解析

武田和義, 力石和英, 最相大輔

平成16年度〜平成18年度科学研究費補助金基盤研究B 研究成果報告書 1 - 110 2008年

Molecular phylogeography of domesticated barley traces expansion of agriculture in the Old World

Daisuke Saisho, Michael D. Purugganan

GENETICS 177 ( 3 ) 1765 - 1776 2007年11月

記述言語：英語出版者・発行元：GENETICS

Barley (Hordeum vulgare ssp. vulgare) was first cultivated 10,500 years ago in the Fertile Crescent and is one of the founder crops of Eurasian agriculture. Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in >250 domesticated barley accessions reveal that landraces found in South and East Asia are genetically distinct from those in Europe and North Africa. A Bayesian population structure assessment method indicates that barley accessions are subdivided into six clusters and that barley landraces from 10 different geographical regions of Eurasia and North Africa show distinct patterns of distribution across these clusters. Using haplotype frequency data, it appears that the Europe/North Africa landraces are most similar to the Near East population (F-ST = 0.15) as well as to wild barley (F-ST = 0.11) and are strongly differentiated from all other Asian populations (F-ST = 0.34-0.74). A neighbor-joining analysis using these F-ST estimates also supports a division between European, North African, and Near East barley types from more easterly Asian accessions. There is also differentiation in the presence of a naked caryopsis and spikelet row number between eastern and western barley accessions. The data support the differential migration of barley from two domestication events that led to the origin of barley - one in the Fertile Crescent and another farther east, possibly at the eastern edge of the Iranian Plateau - with European and North African barley largely originating from the former and much of Asian barley arising from the latter. This suggests that cultural diffusion or independent innovation is responsible for the expansion of agriculture to areas of South and East Asia during the Neolithic revolution.

DOI： 10.1534/genetics.107.079491

酸化ストレスとミトコンドリア機能—二層膜オルガネラの遺伝学 ; オルガネラダイナミクス植物ミトコンドリアダイナミクスと環境応答

Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library from Japanese Malting Barley ‘Haruna Nijo’.

Saisho, D, E. Myoraku, S. Kawasaki, K. Sato, K. Takeda

Breed. Sci. 57 ( 1 ) 29 - 38 2007年3月

草型変異から見たイネとオオムギの栽培化．

浅野賢治, 最相大輔, 芦苅基行, 松岡信

蛋白質核酸酵素 52 ( 15 ) 1931 - 1936 2007年

目黒直樹, 最相大輔, 中園幹生

蛋白質核酸酵素 / 共立出版株式会社 [編] 50 ( 14 ) 1879 - 1882 2005年11月

酸化ストレスとミトコンドリア機能．

目黒直樹, 最相大輔, 中園幹生

蛋白質核酸酵素 50 ( 14 ) 2005年

Spontaneous brassinolide-insensitive barley mutant ‘uzu’ adapted to East Asia

Saisho, D, K. Tanno, M. Chono, I. Honda, H. Kitano, K. Takeda

Breed. Sci. 54 ( 4 ) 409 - 416 2004年12月

「イネ科作物の半わい性形質の機構と利用」-”緑の革命”と渦性オオムギとの比較-

最相大輔

資源生物科学シンポジウム 21st 2004年

A semidwarf phenotype of barley uzu results from a nucleotide substitution in the gene encoding a putative brassinosteroid receptor. 国際誌

Makiko Chono, Ichiro Honda, Haruko Zeniya, Koichi Yoneyama, Daisuke Saisho, Kazuyoshi Takeda, Suguru Takatsuto, Tsuguhiro Hoshino, Yoshiaki Watanabe

Plant physiology 133 ( 3 ) 1209 - 19 2003年11月

記述言語：英語

Brassinosteroids (BRs) play important roles throughout plant growth and development. Despite the importance of clarifying the mechanism of BR-related growth regulation in cereal crops, BR-related cereal mutants have been identified only in rice (Oryza sativa). We previously found that semidwarf barley (Hordeum vulgare) accessions carrying the "uzu" gene, called "uzu" barley in Japan, are non-responding for brassinolide (BL). We then performed chemical and molecular analyses to clarify the mechanisms of uzu dwarfism using isogenic line pairs of uzu gene. The response of the uzu line to BL was significantly lower than that of its corresponding normal line. Measurement of BRs showed that the uzu line accumulates BRs, similar to known BR-insensitive mutants. The marker synteny of rice and barley chromosomes suggests that the uzu gene may be homologous to rice D61, a rice homolog of Arabidopsis BR-insensitive 1 (BRI1), encoding a BR-receptor protein. A barley homolog of BRI1, HvBRI1, was isolated by using degenerate primers. A comparison of HvBRI1 sequences in uzu and normal barley varieties showed that the uzu phenotype is correlated with a single nucleotide substitution. This substitution results in an amino acid change at a highly conserved residue in the kinase domain of the BR-receptor protein. These results may indicate that uzu dwarfism is caused by the missense mutation in HvBRI1. The uzu gene is being introduced into all hull-less barley cultivars in Japan as an effective dwarf gene for practical use, and this is the first report about an agronomically important mutation related to BRs.

DOI： 10.1104/pp.103.026195

14. 半矮性オオムギ"渦"は農業上重要なブラシノステロイド関連変異体である

蝶野真喜子, 本多一郎, 銭谷晴子, 米山弘一, 最相大輔, 武田和義, 高津戸秀, 星野次汪, 渡邊好昭

植物化学調節学会研究発表記録集 38th ( 38 ) 31 - 31 2003年10月

記述言語：日本語出版者・発行元：植物化学調節学会

Brassinosterois (BRs) play important roles throughout plant growth and development. Despite the importance of clarifying the mechanism of BR-related growth regulation in cereal crops, BR-related cereal mutants have been identified only in rice. We previously found that semi-dwarf barley accessions carrying the "uzu" gene, called "uzu" barley in Japan, are non-responding for brassinolide (BL). We then performed chemical and molecular analyses to clarify the mechanisms of uzu dwarfism using isogenic line pairs of uzu gene. The response of the uzu line to BL was significantly lower than that of its corresponding normal line. Measurement of BRs showed that the uzu line accumulates BRs, similar to known BR-insensitive mutants. The marker synteny of rice and barley chromosomes suggests that the uzu gene may be homologous to rice D61,a rice homolog of Arabidopsis BR-insensitive 1 (BRI1), encoding a BR-receptor protein. A barley homolog of BRI1,HvBRI1,was isolated by using degenerate primers. A comparison of HvBRI1 sequences in uzu and normal barley varieties showed that the uzu phenotype is correlated with a single nucleotide substitution. This substitution results in an amino acid change at a highly conserved residue in the kinase domain of the BR-receptor protein. These results may indicate that uzu dwarfism is caused by the missense mutation in HvBRI1. The uzu gene is being introduced into all hull-less barley cultivars in Japan as an effective dwarf gene for practical use, and this is the first report about an agronomically important mutation related to BRs.

DOI： 10.18978/jscrpanb.38.0_31

渦遺伝子がオオムギ未熟胚由来カルスからの植物体再分化に及ぼす影響

力石和英, 最相大輔, 前川雅彦, 武田和義

育種学研究 2003年

オオムギ半矮性遺伝子‘渦’はブラシノステロイド非感受性変異に起因する．

最相大輔, 蝶野真喜子, 本多一郎, 渡邊好昭, 丹野研一, 北野英己, 武田和義

日本植物生理学会年会およびシンポジウム講演要旨集 44 S71 - S71 2003年

記述言語：英語掲載種別：研究発表ペーパー・要旨（国際会議）出版者・発行元：日本植物生理学会

オオムギの渦性は，3H染色体上に座乗する単一劣性遺伝様式を示す遺伝子uzuによって支配される半矮性形質を示す．渦性系統は耐倒伏性に優れた草型を示し、多肥密植条件で多収となる．同様の草型を呈し，「緑の革命」として知られるイネ及びコムギの半矮性形質は，何れもジベレリンに関わる変異によってもたらされているが，オオムギ渦性は古くからジベレリンとは無関係の変異であることが知られており，その遺伝的な制御機構については不明であった．本研究では，uzu遺伝子の同質遺伝子系統対を用いて形態的搏Iな側面から詳細に解析を行った．その結果，渦性系統は高温生育条件下では第一節間の伸長が抑制されるdm型の節間伸長様式を示すこと，ブラシノライド投与に対する応答性が低く，且つ内在性のブラシノステロイド類が過剰に蓄積していることが明らかとなった．これらの形質は，近年イネで報告されたブラシノステロイド非感受性変異体d61と酷似していた．そこで，並性（正常型）と渦性オオムギからD61相同遺伝子を単離し，その塩基配列を比較したところ，C末端側の機能性領域の中にアミノ酸変異につながる一塩基置換を見出した．この変異は，遺伝地図上で渦性と同座に位置付けられ，皮裸性や播性程度の異なる複数の渦性系統が同一の変異を有することを見出した．従って，オオムギ渦性はブラシノステロイドレセプターの変異に起因すると結論した．

DOI： 10.14841/jspp.2003.0.185.0

CiNii Article

塩ストレス条件下における塩抵抗性オオムギ根cDNAのEXPRESSED SEQUENCE TAG(EST)解析

杉本学, 矢野健太郎, 南角奈美, 最相大輔, 佐藤和広, 武田和義

日本分子生物学会年会プログラム・講演要旨集 26th 2003年

オオムギ半矮性遺伝子uzu座に連鎖するマイクロサテライトマーカーの遺伝的多様性

丹野研一, 南角奈美, 最相大輔, 武田和義

育種学研究 = Breeding research 4 399 - 399 2002年8月

醸造用オオムギ"はるな二条"のBACライブラリー構築

最相大輔, 川崎信二, 佐藤和広, 武田和義

育種学研究 = Breeding research 4 269 - 269 2002年8月

オオムギにおける半矮性「渦」の特性解析

武田和義, 最相大輔, 力石和英, 北野英巳

育種学研究 4 356 2002年

The gene for alternative oxidase-2 (AOX2) from Arabidopsis thaliana consists of five exons unlike other AOX genes and is transcribed at an early stage during germination

D Saisho, M Nakazono, KH Lee, N Tsutsumi, S Akita, A Hirai

GENES & GENETIC SYSTEMS 76 ( 2 ) 89 - 97 2001年4月

記述言語：英語出版者・発行元：GENETICS SOC JAPAN

We investigated the expressions of genes for alternative oxidase (AOX1a, AOX1b, AOX1c and AOX2) and genes for cytochrome c oxidase (COX5b and COX6b) during germination of Arabidopsis thaliana, and examined oxygen uptakes of the alternative respiration and the cytochrome respiration in imbibed Arabidopsis seeds. A Northern blot analysis showed that AOX2 mRNA has already accumulated in dry seeds and subsequently decreased, whereas accumulation of AOX1a mRNA was less abundant from 0 hours to 48 hours after imbibition and then increased. The increase of the capacity of the alternative pathway appeared to be dependent on the expressions of both AOX2 and AOX1a. On the other hand, steady-state mRNA levels of COX5b and COX6b were gradually increased during germination, and the capacity of the cytochrome pathway was correlated with the increase of expressions of the COX genes. Antimycin A, the respiratory inhibitor, strongly increased the expression of AOX1a but had no effect on the expression of AOX2. A 5'RACE analysis showed that AOX2 consists of five exons, which is different from the case of most AOX genes identified so far. Analysis of subcellular localization of AOX2 using green fluorescent protein indicated that the AOX2 protein is imported into the mitochondria.

DOI： 10.1266/ggs.76.89

ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana

D Saisho, M Nakazono, N Tsutsumi, A Hirai

JOURNAL OF PLANT PHYSIOLOGY 158 ( 2 ) 241 - 245 2001年2月

記述言語：英語出版者・発行元：URBAN & FISCHER VERLAG

The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOX1a transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NaN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.

Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions

M Nakazono, H Tsuji, YH Li, D Saisho, S Arimura, N Tsutsumi, A Hirai

PLANT PHYSIOLOGY 124 ( 2 ) 587 - 598 2000年10月

記述言語：英語出版者・発行元：AMER SOC PLANT PHYSIOLOGISTS

It is known that alcoholic fermentation is important for survival of plants under anaerobic conditions. Acetaldehyde, one of the intermediates of alcoholic fermentation, is not only reduced by alcohol dehydrogenase but also can be oxidized by aldehyde dehydrogenase (ALDH). To determine whether ALDH plays a role in anaerobic metabolism in rice (Oryza sativa L. cv Nipponbare), we characterized a cDNA clone encoding mitochondrial ALDH from rice (Aldh2a). Analysis of sub-cellular localization of ALDH2a protein using green fluorescent protein and an in vitro ALDH assay using protein extracts from Escherichia coli cells that overexpressed ALDH2a indicated that ALDH2a functions in the oxidation of acetaldehyde in mitochondria. A Southern-blot analysis indicated that mitochondrial ALDH is encoded by at least two genes in rice. We found that the Aldh2a mRNA was present at high levels in leaves of dark-grown seedlings, mature leaf sheaths, and panicles. It is interesting that expression of the rice Aldh2a gene, unlike the expression of the tobacco (Nicotiana tabacum) Aldh2a gene, was induced in rice seedlings by submergence. Experiments with ruthenium red, which is a blocker of Ca2+ fluxes in rice as well as maize (Zea mays), suggest that the induction of expression of Adh1 and Pdc1 by low oxygen stress is regulated by elevation of the cytosolic Ca2+ level. However, the induction of Aldh2a gene expression may not be controlled by the cytosolic Ca2+ level elevation. A possible involvement of ALDH2a in the submergence tolerance of rice is discussed.

DOI： 10.1104/pp.124.2.587

オオムギのcDNAシークエンス解析 : ビールオオムギ「はるな二条」と日本在来品種「赤神力」との比較

最相大輔, 佐藤和広, 高橋秀和, 武田和義

育種学研究 = Breeding research 2 ( 2 ) 32 - 32 2000年9月

Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation: Evidence for involvement of calcium in expression of the alternative oxidase 1a gene

Hiroyuki Tsuji, Mikio Nakazono, Daisuke Saisho, Nobuhiro Tsutsumi, Atsushi Hirai

FEBS Letters 471 ( 2-3 ) 201 - 204 2000年4月

記述言語：英語

We investigated the effect of oxygen on the expressions of respiratory genes encoded in the nuclear and mitochondrial genomes of rice (Oryza sativa L.). Hypoxic treatment decreased the transcript levels of nuclear-encoded, but not mitochondrial-encoded respiratory genes. The effects of ruthenium red (an inhibitor of Ca2+ fluxes from organelles) and/or CaCl2 on plants under hypoxic conditions suggested that Ca2+ is a physiological transducer of a low-oxygen signaling pathway for expression of the alternative oxidase 1a gene (AOX1a), but not for expressions of genes involved in the cytochrome respiratory pathway, in rice. Copyright (C) 2000 Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(00)01411-3

Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation : evidence for involvement of calcium in expression of the alternative oxidase 1a gene.(共著)

FEBS Lett. 471 ( 2-3 ) 201 - 204 2000年4月

Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation: evidence for involvement of calcium in expression of the alternative oxidase la gene

H Tsuji, M Nakazono, D Saisho, N Tsutsumi, A Hirai

FEBS LETTERS 471 ( 2-3 ) 201 - 204 2000年4月

記述言語：英語出版者・発行元：ELSEVIER SCIENCE BV

We investigated the effect of oxygen on the expressionse of respiratory genes encoded in the nuclear and mitochondrial genomes of rice (Oryza sativa L.). Hypoxic treatment decreased the transcript levels of nuclear-encoded, but not mitochondrial-encoded respiratory gents. The effects of ruthenium red (an inhibitor of Ca2+ fluxes from organelles) and/or CaCl2 on plants under hypoxic conditions suggested that Ca2+ is a physiological transducer of a low-oxygen signaling pathway for expression of the alternative oxidase 1a gene (AOX1a), but not for expressions of genes involved in the cytochrome respiratory pathway, in rice. (C) 2000 Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(00)01411-3

低酸素によるイネAOX1a遺伝子の発現抑制には細胞内カルシウムイオンが関与している

辻寛之, 中園幹生, 最相大輔, 堤伸浩, 平井篤志

育種学研究 = Breeding research 2 ( 1 ) 66 - 66 2000年4月

Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions.(共著)

Plant Physiol. 124 ( 2 ) 587 - 598 2000年

Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions

M. Nakazono, H. Tsuji, Y. Li, D. Saisho, S. I. Arimura, N. Tsutsumi, A. Hirai

Plant Physiology 124 ( 2 ) 587 - 598 2000年

記述言語：英語出版者・発行元：American Society of Plant Biologists

DOI： 10.1104/pp.124.2.587

冠水条件下でのイネミトコンドリア遺伝子の発現応答 2. アルデヒド脱水素酵素(ALDH)遺伝子の発現誘導

中園幹生, 辻寛之, 最相大輔, 中園江, 堤伸浩, 平井篤志

育種学研究 1 76 1999年4月

冠水条件下でのイネミトコンドリア遺伝子の発現応答 1. 呼吸系遺伝子の発現

辻寛之, 中園幹生, 最相大輔, 堤伸浩, 平井篤志

育種学研究 1 75 1999年4月

Expression of the alternative oxidase gene is induced by treatment of rice with the bleaching herbicide norflurazon

Daisuke Saisho, Mikio Nakazono, Nobuhiro Tsutsumi, Atsushi Hirai

RICE GENET. NEWSLT. 1999年

CYCLOHEXIMIDE INDUCES TRANSCRIPTION OF THE GENE FOR ALTERNATIVE OXIDASE IN Arabidopsis thaliana

SAISHO Daisuke, TSUTSUMI Nobuhiro, HIRAI Atsushi, NAKAZONO Mikio

Plant and cell physiology 39 S94 - S94 1998年5月

Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature.(共著)

GENE 203 ( 2 ) 121 - 129 1997年12月

Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature

Yusuke Ito, Daisuke Saisho, Mikio Nakazono, Nobuhiro Tsutsumi, Atsushi Hirai

Gene 203 ( 2 ) 121 - 129 1997年12月

記述言語：英語

We identified two genes for alternative oxidase (AOX) from rice. One AOX gene (designated AOX1a) is located approx. 1.9 kb downstream of another AOX gene (designated AOX1b). Comparison of the genomic and cDNA sequences of the two AOX genes showed that the AOX1a gene is interrupted by three introns, as are AOX genes of other plants. On the other hand, two introns are inserted in the AOX1b gene. The predicted AOX1a and AOX1b precursor proteins consist of 332 and 335 amino acid residues, respectively. A genomic Southern hybridization analysis indicated that rice has several AOX genes other than the two tandem-arranged AOX genes. Steady-state mRNA levels of both of the genes for AOX1a and AOX1b were increased under low temperature (4°C). However, no difference in the pattern of induction of transcription between the genes for AOX1a and AOX1b was observed.

DOI： 10.1016/S0378-1119(97)00502-7

Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature. (共著)

GENE 203 ( 2 ) 121 - 129 1997年12月

Characterization of the gene family for alternative oxidase from Arabidopsis thaliana.(共著)

Plant Mol. Biol. 35 ( 5 ) 585 - 596 1997年11月

Characterization of the gene family for alternative oxidase from Arabidopsis thaliana

Daisuke Saisho, Eiji Nambara, Satoshi Naito, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

Plant Molecular Biology 35 ( 5 ) 585 - 596 1997年11月

記述言語：英語

We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a, AOX1b, AOX1c and AOX2) of the AOX genes. Interestingly, two of the AOX genes (AOX1a and AOX1b) were located in tandem in a ca. 5 kb region on one of the chromosomes of Arabidopsis. Comparison between genomic and cDNA sequences of the four AOX genes showed that all AOX genes are divided by three introns and the positions of the introns in AOX1a, AOX1b, AOX1c and AOX2 are the same. We examined whether expression of Arabidopsis AOX genes, like the tobacco AOX1a gene, is enhanced by treatment with antimycin A, an inhibitor of complex III in the mitochondrial respiratory chain. We found that, in young plants, the amount of Arabidopsis AOX1a mRNA was dramatically increased by addition of antimycin A, while the transcription of the other three genes (AOX1b, AOX1c and AOX2) did not respond to antimycin A. Amplification by RT-PCR showed that AOX1a and AOX1c were expressed in all organs examined (flowers and buds, stems, rosette, and roots of 8-week old plants). In contrast, transcripts of AOX1b were detected only in the flowers and buds, and transcripts of AOX2 were detected mainly in stems, rosette and roots. These results suggested that transcriptions of the four genes for alternative oxidase of Arabidopsis are differentially regulated.

DOI： 10.1023/A:1005818507743