Faculty Profiles - TOYODA Kazuhiro

MISC - TOYODA Kazuhiro

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(117) Tolerance to Bacterial Infection in Transgenic Tobacco Plants Expressing PsAPY1(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Nagai H., Tsujimura K., Takahashi H., Kiba K., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 232 - 233 2006.11

(404) Recognition of Pseudomonas syringae pv. tabaci Flagellin in Arabidopsis thahana(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Naito K., Miyoshi R., Ishiga Y., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 310 - 310 2006.11

(431) Analysis of Virulence Factors and Screening of Swarming-specific Genes in Pseudomonas syringae pv. tabaci(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Asai S., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 317 - 317 2006.11

(119) Cytological Analyses of Mycosphaerella pinodes Infection and Differences in Host Response among Medicago truncatula Ecotypes(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Suzuki T., Maeda A., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 233 - 233 2006.11

(120) Phenotypic Characterization of Medicago truncatula Mutants with Decreased Susceptibility to Mycosphaerella pinodes Infection(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Maeda A., Suzuki T., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 233 - 233 2006.11

(430) Effect of Chemotaxis in Motility and Virulence of Pseudomonas syringae pv. tabaci(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Ogawa Y., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 317 - 317 2006.11

(121) Pea Cell-wall-redox Enzymes and Recognition/Response to Pathogens(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Kasai T., Osaki O., Suzuki T., Toyoda K., Kiba A., Inagaki Y., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 234 - 234 2006.11

Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci

F Taguchi, K Takeuchi, E Katoh, K Murata, T Suzuki, M Marutani, T Kawasaki, M Eguchi, S Katoh, H Kaku, C Yasuda, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

CELLULAR MICROBIOLOGY 8 ( 6 ) 923 - 938 2006.6

Language：English Publisher：BLACKWELL PUBLISHING

A glycosylation island is a genetic region required for glycosylation. The glycosylation island of flagellin in Pseudomonas syringae pv. tabaci 6605 consists of three orfs: orf1, orf2 and orf3. Orf1 and orf2 encode putative glycosyltransferases, and their deletion mutants, Delta orf1 and Delta orf2, exhibit deficient flagellin glycosylation or produce partially glycosylated flagellin respectively. Digestion of glycosylated flagellin from wild-type bacteria and non-glycosylated flagellin from Delta orf1 mutant using aspartic N-peptidase and subsequent HPLC analysis revealed candidate glycosylated amino acids. By generation of site-directed Ser/Ala-substituted mutants, all glycosylated amino acid residues were identified at positions 143, 164, 176, 183, 193 and 201. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) analysis revealed that each glycan was about 540 Da. While all glycosylation-defective mutants retained swimming ability, swarming ability was reduced in the Delta orf1, Delta orf2 and Ser/Ala-substituted mutants. All glycosylation mutants were also found to be impaired in the ability to adhere to a polystyrene surface and in the ability to cause disease in tobacco. Based on the predicted tertiary structure of flagellin, S176 and S183 are expected to be located on most external surface of the flagellum. Thus the effect of Ala-substitution of these serines is stronger than that of other serines. These results suggest that glycosylation of flagellin in P. syringae pv. tabaci 6605 is required for bacterial virulence. It is also possible that glycosylation of flagellin may mask elicitor function of flagellin molecule.

DOI： 10.1111/j.1462-5822.2005.0674.x

Web of Science

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Functional analysis of Gac two-component system in P syringae pv. tabaci

M. Marutani, Y. Ogawa, F. Taguchi, Y. Inagaki, K. Toyoda, T. Shiraishi, Y. Ichinose

PHYTOPATHOLOGY 96 ( 6 ) S74 - S74 2006.6

Ultrastructural Features of Mycosphaerella pinodes Infection and Differences in Host Susceptibility Responses among of Medicago truncatula (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Suzuki T., Maeda A., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 65 - 66 2006.2

Functional Analysis of AtOPR1 and 2 in the Interactions with Fungal Pathogens using AtOPR1-and AtOPR2-Overexpressed Transgenic Arabidopsis (2) (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Matsui H., Itazaki M., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 66 - 66 2006.2

Analysis of AHL-production Defective Mutant, Δorf3 of Pseudomonas syringae pv. tabaci (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Taguchi F., Ogawa Y., Suzuki T., Takeuchi K., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 79 - 79 2006.2

HR-inducible Domain of Pseudomonas syringae pv. tabaci Flagellin in Arabidopsis thaliana (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Naito K., Ishiga Y., Taguchi F., Takeuchi K., Katoh E., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 78 - 79 2006.2

Signal Transduction through the Plant Cell Wall-plasma Membrane Continuum (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Hirai Y., Toyoda K., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 66 - 66 2006.2

Induction of defense responses in pea tissues by inorganic phosphate.

Kawahara, T, Namba, H, Toyoda, K, Kasai, T, Sugimoto, M, Inagaki, Y, Ichinose, Y, Shiraishi, T

J. Gen. Plant Pathol. 72 ( 3 ) 129 - 136 2006

A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms

KIBA Akinori, TOYODA Kazuhiro, YOSHIOKA Kazuaki, TSUJIMURA Kami, TAKAHASHI Hirotaka, ICHINOSE Yuki, TAKEDA Tadahiro, KATO Toshiaki, SHIRAISHI Tomonori

J Gen Plant Pathol 72 ( 4 ) 238 - 246 2006

Peas extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen.

Kasai, T, Suzuki, T, Ono, K, Ogawa, K, Inagaki, Y, Ichinose, Y, Toyoda, K, Shiraishi, T

J. Gen. Plant Pathol. 72 ( 5 ) 265 - 272 2006

A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis

Taguchi Fumiko, Ogawa Yujiro, Takeuchi Kasumi, Suzuki Tomoko, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

Journal of Bacteriology 188 ( 24 ) 8376 - 8384 2006

A binding protein for fungal signal molecules in cell wall of Pisum sativum.

Kiba, A, Ohgawara, T, Toyoda, K, Inoue-Ozaki, M, Takeda, T, Rao, U.S, Kato, T, Ichinose, Y, Shiraishi, T

J. Gen. Plant Pathol. 72 ( 4 ) 228 - 237 2006

AtOPR3高発現シロイヌナズナを用いた植物病原菌相互作用におけるOPR subgroup 2の機能解析

松井英譲, 豊田和弘, 稲垣善茂, 白石友紀, 一瀬勇規

日本植物病理学会報 72 ( 4 ) 2006

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