Faculty Profiles - TOYODA Kazuhiro

MISC - TOYODA Kazuhiro

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Effect of Harpin from Four Pathovars of Pseudomonas syringae on Pea Defense Responses (Short Communication) :

TANAKA Rui, TAGUCHI Fumiko, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general plant pathology : JGPP 67 ( 2 ) 148 - 151 2001

Language：English Publisher：Phytopathological Society of Japan

To investigate the role of the proteinaceous elicitor, harpin, on host and nonhost plants, we isolated the harpin-coding gene, hrpZ, from Pseudomonas syringae pvs. pisi, glycinea, tabaci and tomato. Effects of the recombinant harpin proteins on pea plants were analyzed and compared with the effects of the corresponding bacterial treatment. After inoculation of pea with pea pathogen P. syringae pv. pisi, the bacterial population increased and the accumulation of PAL-mRNA and pisatin was inhibited. The nonpathogenic pathovars, glycinea, tabaci and tomato induced both defense responses in pea. However, none of the harpins induced the hypersensitive reaction or accumulation of PAL-mRNA and pisatin in pea. Harpins from P. syringae pvs. glycinea, tomato and pisi did induce these defense responses in tobacco, however, suggesting that externally applied harpins either are not recognized or are nonfunctional in pea plants. (Received July 27, 2000 ; Accepted February 21, 2001)

DOI： 10.1007/PL00013001

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Other Link： http://id.nii.ac.jp/1141/00138226/

Effect of methyl jasmonate on harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of defense genes in tobacco suspension cultured BY-2 cells.

Andi, S, Taguchi, F, Toyoda, K, Shiraishi, T, Ichinose, Y

Plant Cell Physiol. 42 ( 4 ) 446 - 449 2001

Harpin_<psta> from Pseudomonas syringae pv. tabaci is defective and deficient in its expression and HR-inducing activity.

TAGUCHI F.

J. Gen. Plant Pathol. 67 ( 2 ) 116 - 123 2001

初期・表層シグナル伝達系と防御システム

白石友紀, 豊田和弘, 木場章範, 一瀬勇規

化学と生物 39 (10): 686-692 ( 10 ) 686 - 692 2001

The Role of Active Oxygen Species Accompanied with HR by Elicitin in Tobacco Cell Suspension Culture(Abstracts Presented at the Meeting of the Kansai Division)

Takeuchi K., Sasabe M., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 287 - 288 2000.12

Hypersensitive Response-inducing Factors Produced in Pseudomonas syringae : (2) Induction and Suppression of Hypersensitive Reponse by Harpin(Abstracts Presented at the Meeting of the Kansai Division)

Andi S., Taguchi F., Doi R., Tanaka R., Sasabe M., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 292 - 293 2000.12

Cell Wall NTPase in Pea Recognizes the Signal Molecules from Mycosphaerella pinodes(Abstracts Presented at the Meeting of the Kansai Division)

Yoshioka K., Kiba A., Kawahara T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 288 - 288 2000.12

Hypersensitive Response-inducing Factors Produced in Pseudomonas syringae : (3) Isolation and Functional Analysis of Flagellin as an Elicitor Molecule(Abstracts Presented at the Meeting of the Kansai Division)

Taguchi F., Nakajima R., Andi s., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 293 - 293 2000.12

Analysis of Suppressorresponsive Genes in Pea, : (3)Identification of S64 Protein as Oxophytodienoic Acid Reductase(Abstracts Presented at the Meeting of the Kansai Division)

Ishiga Y., Ichinose Y., Toyoda K., Shiraisi T., Yamada T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 288 - 288 2000.12

Interaction between an Integrin-like Protein(s) and a Phosphatidylinositol 4-Phosphate 5-Kinase (PIP5K) in Pea Plasma Membrane(Abstracts Presented at the Meeting of the Kansai Division)

Toyoda K., Okazaki A., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 288 - 288 2000.12

Hypersensitive Response-Inducing Factors Produced in Pseudomonas syringae : (1) Structural and Functional Analysis of Harpin Pseudomonas syringae(Abstracts Presented at the Meeting of the Kansai Division)

Tanaka R., Taguchi F., Andi S., Kinoshita S., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 292 - 292 2000.12

Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

JOURNAL OF PHYTOPATHOLOGY 148 ( 11-12 ) 633 - 636 2000.12

Language：English Publisher：WILEY-BLACKWELL

When epicotyl tissues of pea were treated with a diacylglycerol (DAG) kinase inhibitor (R59022), enhanced induction of the phytoalexin accumulation occurred which was induced by fungal elicitor. The marked induction was associated with a sustained accumulation of phenylalanine ammonia-lyase (PAL)-mRNA and the consequent increase in PAL activity. These results suggest that inhibition of DAG breakdown leads to increased induction of phytoalexin accumulation and support the hypothesis that DAG kinase negatively regulates the signal transduction.

DOI： 10.1046/j.1439-0434.2000.00568.x

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Specific Inhibition of Cell Wall-bound ATPase by the Suppressor from Mycosphaerella pinodes(6) : Cloning and Functional Analysis of Fungal Signal-binding Protein from Pea Plant

Kiba A., Ohgawara T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 66 ( 2 ) 116 - 117 2000.8

Cytochalasin A inhibits the binding of phenylalanine ammonia-lyase mRNA to ribosomes during induction of phytoalexin in pea seedlings

M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 41 ( 2 ) 234 - 238 2000.2

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Cytochalasin A (CA) blocked the accumulation of phytoalexin and phenylalanine ammonia-lyase (PAL)-protein in pea tissues treated with a fungal elicitor but scarcely affected the PAL-mRNA content. Further analysis showed that CA decreased the PAL-mRNA bound to ribosomes. These results indicate that actin filaments are tightly associated with the translational process of the PAL gene.

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Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization

Y Ichinose, K Tiemann, C Schwenger-Erger, K Toyoda, F Hein, T Hanselle, H Cornels, W Barz

ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES 55 ( 1-2 ) 44 - 54 2000.1

Language：English Publisher：VERLAG Z NATURFORSCH

In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanisms at the level of gene activation and deactivation, differential screenings were performed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism. Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNAs provide a variety of tools to investigate molecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

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Importance of AC-rich element on pea phenylalanine ammonia-lyase gene 1 promoter for expression induced by nonpathogenic attack

IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general Plant Pathology 66 ( 2 ) 123 - 127 2000

Language：English Publisher：Phytopathological Society of Japan

Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

DOI： 10.1007/PL00012932

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Other Link： http://id.nii.ac.jp/1141/00138133/

PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS :

KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology 41 s10 2000

INDEPENDENT PATHWAYS OF DEFENSE RESPONSES IN ELICITIN-TREATED TOBACCO BY-2 CELLS :

SASABE Michiko, TAKEUCHI Kasumi, KAMOUN Sophien, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology 41 s85 2000

STRUCTURAL AND FUNCTIONAL ANALYSES OF HARPINS FROM SEVERAL PATHOVARS OF PSEUDOMONAS SYRINGAE :

TANAKA Rui, TAGUCHI Tomiko, KINOSHITA Sayuri, NAKADA Hideki, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology 41 s84 2000

Analysis of Suppressorresponsive Genes in Pea (2) : Molecular Structure and Gene Expression of S64 (Abstracts Presented at the Meeting of the Kansai Division)

Funato A., Ishiga Y., Ichinose Y., Toyoda K., Shiraishi T., Yamada T., Barz W.

Annals of the Phytopathological Society of Japan 65 ( 6 ) 671 - 671 1999.12

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