Faculty Profiles - TOYODA Kazuhiro

J-GLOBAL

デュアル抵抗性蛋白質システムを構成するRRS1およびRPS4の機能解析

非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制機構の解析

石井智也, 渡邉恵, 齊藤晶, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報 85 ( 1 ) 2019

メトミノストロビンの作用機構に関する研究

佐藤穂高, 市成光広, 櫻本和生, 山田晶, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報 85 ( 1 ) 2019

タバコ野火病菌のCDG1はアシルホモセリンラクトンの蓄積を正に制御する

澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

日本植物病理学会報 85 ( 1 ) 2019

抵抗性誘導剤のシーズとして単離したサリチル酸配糖化酵素阻害剤の反応速度論解析

篠原優佳, 渡邉恵, 楠和輝, 谷川友里佳, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 熊谷和夫, 米須清明, 能年義輝

日本植物病理学会報 85 ( 1 ) 2019

エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報 85 ( 1 ) 2019

エンドウの細胞壁画分に見出されたキトサンと結合するタンパク質複合体

松尾実佳, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集 2019 2019

Pseudomonas syringae pv.tabaciのRNAタイプ多剤排出トランスポーターの病原力における機能解析

西村隆史, 一瀬勇規, 原田みのり, 松井英譲, 山本幹博, 能年義輝, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集 2019 2019

微生物分子パターンに対するミナトカモジグサの活性酸素種生成特性の解析

小笠原翼, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 香西雄介, 能年義輝

日本植物病理学会報 85 ( 1 ) 2019

細胞外オキシダティブバースト反応の分子機構:ペルオキシダーゼのスルフェン酸化とその役割

高須瑞穂, 吉岡美樹, 吉岡博文, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

日本植物病理学会報 85 ( 1 ) 2019

植物免疫活性剤インプリマチンDがpattern-triggered immunityに与える影響

山枡一秀, 平田奈美, 福井絢子, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集 2019 2019

Pseudomonas syringaeの菌体密度感知機構の解析(4)菌体密度に応じた遺伝子発現制御

中津有紀子, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集 2018 2018

バイオコントロール細菌Rhizobium vitis VAR03-1株のブドウ根頭がんしゅ病抑制機構の解析

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集 2018 2018

Pseudomonas syringaeの菌体密度感知機構の解析(3)PsyRとアシルホモセリンラクトンによる遺伝子発現制御機構

田阪洋昌, 山本悟, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集 2018 2018

エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

植物微生物研究会研究交流会講演要旨集 28th 2018

ササゲにおける細胞外オキシダティブバースト反応の分子機構

川端真矢, 佐藤穂高, 高須瑞穂, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報 84 ( 1 ) 2018

非病原性Rhizobium vitis VAR03-1株の培養上清がブドウ根頭がんしゅ病菌に及ぼす影響

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報 84 ( 1 ) 2018

Pseudomonas syringaeの菌体密度感知機構の解析(2)

一瀬勇規, 松井英譲, 石賀康博, 山本幹博, 能年義輝, 豊田和弘

日本植物病理学会報 84 ( 1 ) 2018

細菌シグナル伝達因子CDG proteinの機能解析

澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集 2018 2018

鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

日本生化学会大会(Web) 90th ROMBUNNO.3P‐1257 (WEB ONLY) 2017

Pseudomonas syringaeの菌体密度感知機構の解析(1)

山本悟, 藤山友里, 高田基弘, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会報 83 ( 1 ) 2017

非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集 2017 2017

エンドウならびにシロイヌナズナにおけるPAMP誘導性細胞外オキシダティブバースト反応の分子機構

片岡千香子, 三木紅葉, 山崎史織, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集 2017 2017

PAMP誘導性細胞外オキシダティブバースト反応の分子機構

植物微生物研究会研究交流会講演要旨集 27th 2017

ミナトカモジグサにおける植物免疫ホルモン応答性マーカー遺伝子の同定と発現プロファイルの解析

香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 恩田義彦, 持田恵一, 能年義輝

日本植物病理学会報 83 ( 1 ) 2017

エクト型ATPase(PsAPY1)はエンドウ葉におけるPAMP誘導性オキシダティブバースト反応を正に調節し,不適応型の病原菌に対する非宿主抵抗性に関与する

山崎史織, 三木紅葉, 矢尾幸世, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報 83 ( 1 ) 2017

エンドウから分離した細胞壁タンパク質のPAMP応答

三木紅葉, 山崎史織, 片岡千香子, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報 83 ( 1 ) 2017

Pseudomonas syringae pv.tabaci6605におけるAHL合成制御機構並びにMexEFOprN多剤排出ポンプの病原力における役割の解析

澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会報 83 ( 1 ) 2017

シロイヌナズナにおけるPAMP誘導性オキシダティブバースト反応におけるアポプラストの役割について

片岡千香子, 山崎史織, 松尾実佳, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報 83 ( 1 ) 2017

シロイヌナズナにおける細胞外オキシダティブバースト反応の分子機構

松尾実佳, 片岡千香子, 山崎史織, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会報 83 ( 1 ) 2017

非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会報 83 ( 1 ) 2017

デュアル抵抗性蛋白質システムを構成する抵抗性蛋白質RRS1の機能解析

鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

日本植物細胞分子生物学会大会・シンポジウム講演要旨集 34th 145 2016.8

デュアル抵抗性蛋白質システムを構成する抵抗性蛋白質RRS1のロイシンジッパーモチーフの機能解析

鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

日本植物病理学会大会プログラム・講演要旨予稿集 2016 90 2016.3

デュアル抵抗性蛋白質を構成するRPS4およびRRS1の機能解析

鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

日本植物病理学会報 82 ( 1 ) 68(J‐STAGE) 2016

エクト型ATPase(PsAPY1)はエンドウ葉におけるリポ多糖(LPS)誘導性細胞外オキシダティブバースト反応を正に調節する

三木紅葉, 山崎史織, 矢尾幸世, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集 2016 2016

ナス科植物青枯病菌の病原力や増殖を制御する化合物の探索

澤井拓, 一瀬勇規, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 向原隆文, 長田裕之

日本植物病理学会大会プログラム・講演要旨予稿集 2016 2016

非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の発病発現抑制機構の解析

齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集 2016 2016

エクト型ATPase(PsAPY1)の発現を抑制したエンドウ葉におけるジャスモン酸応答性遺伝子の発現

矢尾幸世, 田中佳織, 奥田竜太, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

日本植物病理学会報 82 ( 1 ) 2016

ミナトカモジグサ-紋枯病菌感染系の確立と植物ホルモンによる抵抗性機構の解析

香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 恩田義彦, 持田恵一, 山本幹博, 松井英譲, 一瀬勇規, 豊田和弘, 能年義輝

日本植物病理学会報 82 ( 1 ) 2016

Pseudomonas syringae pv.tabaciにおけるAHL合成制御機構の解析

澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会報 82 ( 1 ) 2016

Pseudomonas syringae pv.syringae B728aの病原力制御因子Vfrの標的遺伝子のクロマチン免疫沈降法による探索(2)

小倉敬右, 田口富美子, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

日本植物病理学会大会プログラム・講演要旨予稿集 2016 2016

サリチル酸はミナトカモジグサの紋枯病菌に対する侵入後抵抗性を高める

香西雄介, 木村麻美子, 渡邉恵, 山中由利恵, 恩田義彦, 持田恵一, 一瀬勇規, 山本幹博, 松井英譲, 豊田和弘, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集 2016 2016

抵抗性誘導剤の同定を目的としたサリチル酸配糖化酵素阻害剤の標的ベース探索

楠和輝, 渡邉恵, 米須清明, 熊谷和夫, 香西雄介, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集 2016 2016

デュアル抵抗性蛋白質システムを構成するRPS4およびRRS1の機能解析

鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 鳴坂義弘

日本植物生理学会年会要旨集 56th 339 2015.3

デュアル抵抗性蛋白質RPS4およびRRS1をコアとする蛋白質複合体の機能解析

鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 鳴坂義弘

日本植物病理学会報 81 ( 1 ) 68 2015

異種β-アミリン酸化酵素遺伝子を過剰発現した組換え体イネにおける生理学的影響

内藤優, 島谷善平, 島谷善平, 青野裕子, 平井宏太, 寺田理枝, OSBOURN Anne, 關光, 松島良, 能年義輝, 豊田和弘, 山本幹博, 一瀬勇規, 稲垣善茂

日本植物生理学会年会要旨集 56th 2015

イネにおける異種β-アミリン酸化酵素遺伝子の過剰発現

内藤優, 島谷善平, 島谷善平, 青野裕子, 平井宏太, 寺田理枝, OSBOURN Anne, 關光, 松島良, 能年義輝, 能年義輝, 豊田和弘, 豊田和弘, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 稲垣善茂, 稲垣善茂, 稲垣善茂

イソプレノイド研究会例会講演要旨集 25th 2015

エクト型ATPase(PsAPY1)の発現を抑制したエンドウの非適応型アブラナ科炭疽病菌に対する応答

矢尾幸世, 三木紅葉, 鈴木智子, 山岸紀子, 吉川信幸, 能年義輝, 能年義輝, 稲垣善茂, 稲垣善茂, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集 2015 2015

植物免疫プライミング剤インプリマチンDの作用解析

福井絢子, 香西雄介, 稲垣善茂, 山本幹博, 一瀬勇規, 豊田和弘, 白須賢, 能年義輝

日本植物病理学会大会プログラム・講演要旨予稿集 2015 2015

サリチル酸配糖化酵素を用いた抵抗性誘導剤の標的ベース探索

谷川友里佳, 渡邉恵, 熊谷和夫, 香西雄介, 山中由利恵, 木村麻美子, 稲垣善茂, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

日本植物病理学会報 81 ( 1 ) 2015

デュアル抵抗性蛋白質システムにおける蛋白質間相互作用の解析 2

鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 高野義孝, 久保康之, 鳴坂義弘

日本植物病理学会大会プログラム・講演要旨予稿集 2014 61 2014.5

デュアル抵抗性蛋白質システムにおけるタンパク質間相互作用の解析

鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 鳴坂義弘

日本植物生理学会年会要旨集 55th 320 2014.3

エクト型ATPase(PsAPY1)高発現タバコに付与される耐病性の分子機構

中桐萌, 木場章範, 能年義輝, 能年義輝, 稲垣善茂, 稲垣善茂, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集 2014 2014

エクト型ATPase(PsAPY1)の発現を抑制したエンドウの褐紋病菌に対する応答

矢尾幸世, 高木麻衣, 田中佳織, 山岸紀子, 吉川信幸, 能年義輝, 能年義輝, 稲垣善茂, 稲垣善茂, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

日本植物病理学会大会プログラム・講演要旨予稿集 2014 2014

Suppression of mRNAs for lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase (OPR) in pea reduces sensitivity to the phytotoxin coronatine and disease development by Mycosphaerella pinodes

TOYODA Kazuhiro, KAWANISHI Yuriko, KAWAMOTO Yuriko, KURIHARA Chiaki, YAMAGISHI Noriko, TAMURA Akihiro, YOSHIKAWA Nobuyuki, INAGAKI Yoshishige, ICHINOSE Yuki, SHIRAISHI Tomonori

79 ( 5 ) 321 - 334 2013.9

新説デュアル抵抗性蛋白質システムの作用機作の解明

鳴坂真理, 豊田和弘, 白須賢, 白石友紀, 鳴坂義弘

日本分子生物学会年会プログラム・要旨集(Web) 36th 1P-0488 (WEB ONLY) 2013

3S-Dp02 New Research Approach of Iron-oxidizing Bacterium, Gallionella ferruginea

Suzuki Tomoko, Hashimoto Hideki, Ishihara Hiromichi, Matsumoto Nobuyuki, Akae Yuta, Toyoda Kazuhiro, Shiraishi Tomonori, Kunoh Hitoshi, Takada Jun

65 181 - 181 2013

細胞外ATPによるオキシダティブバースト反応の制御

田中佳織, 豊田和弘, 稲垣善茂, 一瀬勇規, 白石友紀

日本植物病理学会報 79 ( 1 ) 2013

ササゲにおけるペルオキシダーゼ依存性細胞外オキシダティブバースト反応の分子機構

茶谷真菜美, 大河摩祐, 田中佳織, 稲垣善茂, 一瀬勇規, 豊田和弘, 白石友紀

日本植物病理学会大会プログラム・講演要旨予稿集 2013 2013

シロイヌナズナ葉に存在する内生サプレッサー

川崎達弘, 安倍幸子, 能年義輝, 稲垣善茂, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

植物微生物研究会研究交流会講演要旨集 23rd 2013

A volatile substance from Talaromyces sp promotes the plant growth and blocks the disease development on several plants

T. Shiraishi, Y. Yamagiwa, P. T. Le, K. Maeda, Y. Inagaki, Y. Ichinose, M. Hyakumachi, K. Toyoda

PHYTOPATHOLOGY 101 ( 6 ) S165 - S166 2011.6

Metabolic analysis of transgenic rice introduced Avena strigosa beta-Amyrin Shynthase 1

Ikeda Kousuke, Geisler Katrin, Aono Yuuko, Osbourn Anne, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki, Inagaki Yoshishige

Plant and Cell Physiology Supplement 2011 0763 - 0763 2011

Publisher：The Japanese Society of Plant Physiologists

Triterpenoid saponins are sugar-modified triterpene derivatives that have various functions, anti-cancer, anti-oxidant, and anti-microbial. Cereals and grasses are generally deficient in this secondary metabolite except for oats. Our final goal is that the anti-microbial saponin is available for the rice. We reported already that 1) we found twelve Oxidosqualene cyclase homologues in rice genome using knowledge in oats, but it was likely that no one was involved in the triterpenoid biosynthesis pathway, 2) we also found twelve CYP51 sterol demethylase homologues in rice genome. It was likely that one is for rice steroids biosynthesis pathway, and another is for rice triterpeneoids biosynthesis pathway. Here, we report that 1) we made several transgenic rice lines introduced Avena strigosa beta-Amyrin Synthase 1 gene, 2) analyzed the triterpenoids and their intermediates extracted from the transgenic lines by GC/MS, 3) and demonstrated the accumulation of only beta-Amyrin in the transgenic rice without the other intermediates and/or the triterpenoids.

DOI： 10.14841/jspp.2011.0.0763.0

デュアルR‐遺伝子システムによる病原菌認識機構の解明

鳴坂真理, 白須賢, 久保康之, 豊田和弘, 白石友紀, 岩渕雅樹, 鳴坂義弘

日本植物病理学会大会プログラム・講演要旨予稿集 2010 81 2010.3

Effect of FFC ceramic water on the infection process of a fungal pathogen

Scientific Reports of the Faculty of Agriculture, Okayama University 99 27 - 34 2010.2

Enhancement of growth and yield of barley by the soil conditioner FFC-ace

Scientific Reports of the Faculty of Agriculture, Okayama University 99 13 - 20 2010.2

Bacterial DNA activates immunity in Arabidopsis thaliana.

Yakushiji, S, Ishiga, Y, Inagaki, Y, Toyoda, K, Shiraishi, T, Ichinose, Y

J. Gen. Plant Pathol. 75 ( 3 ) 227 - 234 2009

Molecular analysis for CYP51 sterol demethylase in rice

Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki, Osbourn Anne

Plant and Cell Physiology Supplement 2009 0206 - 0206 2009

Publisher：The Japanese Society of Plant Physiologists

Triterpenoid saponins are suger-modified triterpene derivatives that have various functions, anti-cancer, anti-oxidant, and anti-microbial. Cereals and grasses are generally deficient in this secondary metabolite with the exception of oats. Our final goal is that the anti-microbial saponin is available for rice. We reported already that 1) triterpenoid saponins and the intermediates were not detected in rice young seedling by LC/MS and GC/MS analyses, 2) we found twelve Oxidosqualene cyclase homologues in rice genome using knowledge in oats, but it was likely that no one was involved in the triterpeneoid biosynthesis pathway. Here, we report that we found twelve OsCYP51 homologues in rice genome that are in next step of the pathway, and it is likely that one is for rice steroids biosynthesis pathway, and another is for rice triterpeneoids biosynthesis pathway, by genome structure and gene expression analyses.

DOI： 10.14841/jspp.2009.0.0206.0

Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana.

(129) Molecular Analysis for Oxidosqualene Cyclase in Saponin Biosynthesis Pathway of Rice(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Inagaki Y., Imaoka A., Fujita K., Arase S., Toyoda K., Shiraishi T., Ichinose Y., Osbourn Anne

Annals of the Phytopathological Society of Japan 74 ( 3 ) 201 - 201 2008.8

(127) Functional Analysis of Cell Wall-associated apyrase MtAPY1; 1 in Medicago truncatula(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Shiobara T., Fujita K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 74 ( 3 ) 200 - 200 2008.8

(307) Functional Analysis of Flagellar Motility and Elicitor Activity of Flagellin in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Ichinose Y., Naito K., Taguchi F., Suzuki T., Inagaki Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 74 ( 3 ) 248 - 249 2008.8

(400) Genes Expressed in Tissue-cultured Seedlings of Mountain Laurel (Kalmia latifolia L.) with Colonizing Streptomyces padanus AOK30 (II)(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Meguro A., Ogiyama H., Hasegawa S., Nishimura T., Kunoh H., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 74 ( 3 ) 273 - 274 2008.8

(128) Genes Expressed in Leaves of Medicago truncatula in Response to Fungal Suppressor from Mycosphaerella pinodes(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Toyoda K., Kawanishi Y., Matsuzaki M., Inagaki Y., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 74 ( 3 ) 200 - 201 2008.8

(309) Plant Recognizes Bacterial DNA and Induces Defense Responses(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Yakushiji S., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 74 ( 3 ) 249 - 249 2008.8

(3)Mycosphaerella pinodes Infection and Differences in Host Disease Resistance among Medicago truncatula Ecotypes(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

Minakuchi Y., Maeda A., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 74 ( 1 ) 56 - 56 2008.2

(4)Heterologous Expressoin of Medicago truncatula Mtapy1;1 Gene in Nicotiana benthamiana and Characterization of Potential Interactor(s) for Cell Wall Apyrase(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

Komatsu Y., Nagai H., Kawakami E., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 74 ( 1 ) 57 - 57 2008.2

(81)Genes Expressed in Tissue-cultured Seedlings of Mountain Laurel (Kalmia latifolia L.) with Colonizing Endophytic Streptomyces padanus AOK30(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

Meguro A., Ogiyama H., Hasegawa S., Nishimura T., Kunoh H., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 74 ( 1 ) 77 - 77 2008.2

(71)Functional Analysis of Flagellar Motility and Elicitor Activity of Flagellar Protein Flagellin in Pseudomonas syringae pv. tabaci(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

Naito K., Taguchi F., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 74 ( 1 ) 75 - 75 2008.2

Higashi, K, Ishiga, Y, Inagaki, Y, Toyoda, K, Shiraishi, T, Ichinose, Y

Mol. Genet. Genomics 279 ( 3 ) 303 - 312 2008

Molecular analysis for genome structure and gene expression of Oxidosqualene Cyclase genes in rice seedling

Yoshishige Inagaki, Yukako Mutsukado, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Anne Osbourn

GENES & GENETIC SYSTEMS 82 ( 6 ) 521 - 521 2007.12

(364) Activity of Bacterial DNA to Induce Plant Immunity(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Yakushiji S., Ishiga Y., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 272 - 272 2007.8

(134) Rer1, a Novel Gene Induced during Establishment of Susceptibility in Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Tanaka Y., Yoshihiro M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 210 - 210 2007.8

(133) Heterologous Expression and Functional Analyses of Four Medicago truncatula Apyrase Genes(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Nagai H., Kawakami E., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 210 - 210 2007.8

(365) Effect of Iron Acquisition on the Swarming Motility in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Kawamoto K., Taguchi F., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 272 - 272 2007.8

(132) Transcnptional Profiles of Medicago truncatula Cultured Cells Treated with a Fungal Suppressor from Mycosphaerella pinodes and Their Comparison with Those of the Differentiated Leaf Tissues(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Matsuzaki M., Matsuo K., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 209 - 210 2007.8

(361) Modulation of Defense Signal Transduction by Flagellin-induced WRKY41 Transcription Factor in Arabidopsis thaliana(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Higashi K., Ishiga Y., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 271 - 271 2007.8

(362) Functional Analysis of Glycosyltransferases for Flagellin Glycosylation in Pseudomonas syringae(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Tsunemi K., Taguchi F., Yasuda C., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 271 - 272 2007.8

(363) Response to Pathogen in Arabidopsis Variegated var2 Mutant(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Aono M., Miura E., Kato Y., Toyoda K., Inagaki Y., Shiraishi T., Sakamoto W., Ichinose Y.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 272 - 272 2007.8

(360) Function of Flagellar Motor Proteins and Virulence of Motor-Defective Mutants in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the Annual Meeting of the Society)

Kenmotsu T., Taguchi F., Suzuki T., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 73 ( 3 ) 271 - 271 2007.8

Analysis of Flagellum Motor-defective Mutants of Pseudomonas syringae pv. tabaci(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

Kenmotsu T., Taguchi F., Toyoda K., Shiraishi T., Ichonose Y.

Annals of the Phytopathological Society of Japan 73 ( 1 ) 72 - 72 2007.2

Regulation of Defense Responses in Medicago truncatula Cells by Fungal Signal Molecules from Mycosphaerella pinodes(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

Matsuo K., Matsuzaki M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 73 ( 1 ) 54 - 54 2007.2

Functional Analysis of the Flagellin-induced Gene Encoding WRKY41 Transcription Factor in Arabidopsis thaliana(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

Higashi K., Ishiga Y., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 73 ( 1 ) 72 - 72 2007.2

N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana.

Naito, K, Ishiga, Y, Toyoda, K, Shiraishi, T, Ichinose, Y

J. Gen. Plant Pathol. 73 ( 4 ) 281 - 285 2007

Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula Ecotypes.

Suzuki, T, Maeda, A, Hirose, M, Ichinose, Y, Toyoda, K, Shiraishi, T

J. Electr. Microsc. Technol. Med. Biol. 20 ( 2 ) 167 - 168 2007

(116) Plant Cell Wall Apyrase Recognizes and Responses to Diverse Pathogen-associated Molecular Patterns (PAMPs)(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Takahashi H., Hirakawa Y., Morishita K., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 232 - 232 2006.11

(118) Expression of Defense Related Genes in Medicago truncatula and Its Regulation by Fungal Signal Molecules from Mycosphaerella pinodes(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Matsuzaki M., Maeda A., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 233 - 233 2006.11

(117) Tolerance to Bacterial Infection in Transgenic Tobacco Plants Expressing PsAPY1(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Nagai H., Tsujimura K., Takahashi H., Kiba K., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 232 - 233 2006.11

(404) Recognition of Pseudomonas syringae pv. tabaci Flagellin in Arabidopsis thahana(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Naito K., Miyoshi R., Ishiga Y., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 310 - 310 2006.11

(431) Analysis of Virulence Factors and Screening of Swarming-specific Genes in Pseudomonas syringae pv. tabaci(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Asai S., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 317 - 317 2006.11

(119) Cytological Analyses of Mycosphaerella pinodes Infection and Differences in Host Response among Medicago truncatula Ecotypes(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Suzuki T., Maeda A., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 233 - 233 2006.11

(120) Phenotypic Characterization of Medicago truncatula Mutants with Decreased Susceptibility to Mycosphaerella pinodes Infection(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Maeda A., Suzuki T., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 233 - 233 2006.11

(430) Effect of Chemotaxis in Motility and Virulence of Pseudomonas syringae pv. tabaci(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Ogawa Y., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 317 - 317 2006.11

(121) Pea Cell-wall-redox Enzymes and Recognition/Response to Pathogens(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

Kasai T., Osaki O., Suzuki T., Toyoda K., Kiba A., Inagaki Y., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 4 ) 234 - 234 2006.11

Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci

F Taguchi, K Takeuchi, E Katoh, K Murata, T Suzuki, M Marutani, T Kawasaki, M Eguchi, S Katoh, H Kaku, C Yasuda, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

CELLULAR MICROBIOLOGY 8 ( 6 ) 923 - 938 2006.6

Language：English Publisher：BLACKWELL PUBLISHING

A glycosylation island is a genetic region required for glycosylation. The glycosylation island of flagellin in Pseudomonas syringae pv. tabaci 6605 consists of three orfs: orf1, orf2 and orf3. Orf1 and orf2 encode putative glycosyltransferases, and their deletion mutants, Delta orf1 and Delta orf2, exhibit deficient flagellin glycosylation or produce partially glycosylated flagellin respectively. Digestion of glycosylated flagellin from wild-type bacteria and non-glycosylated flagellin from Delta orf1 mutant using aspartic N-peptidase and subsequent HPLC analysis revealed candidate glycosylated amino acids. By generation of site-directed Ser/Ala-substituted mutants, all glycosylated amino acid residues were identified at positions 143, 164, 176, 183, 193 and 201. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) analysis revealed that each glycan was about 540 Da. While all glycosylation-defective mutants retained swimming ability, swarming ability was reduced in the Delta orf1, Delta orf2 and Ser/Ala-substituted mutants. All glycosylation mutants were also found to be impaired in the ability to adhere to a polystyrene surface and in the ability to cause disease in tobacco. Based on the predicted tertiary structure of flagellin, S176 and S183 are expected to be located on most external surface of the flagellum. Thus the effect of Ala-substitution of these serines is stronger than that of other serines. These results suggest that glycosylation of flagellin in P. syringae pv. tabaci 6605 is required for bacterial virulence. It is also possible that glycosylation of flagellin may mask elicitor function of flagellin molecule.

DOI： 10.1111/j.1462-5822.2005.0674.x

A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis

Functional analysis of Gac two-component system in P syringae pv. tabaci

M. Marutani, Y. Ogawa, F. Taguchi, Y. Inagaki, K. Toyoda, T. Shiraishi, Y. Ichinose

PHYTOPATHOLOGY 96 ( 6 ) S74 - S74 2006.6

Ultrastructural Features of Mycosphaerella pinodes Infection and Differences in Host Susceptibility Responses among of Medicago truncatula (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Suzuki T., Maeda A., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 65 - 66 2006.2

Functional Analysis of AtOPR1 and 2 in the Interactions with Fungal Pathogens using AtOPR1-and AtOPR2-Overexpressed Transgenic Arabidopsis (2) (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Matsui H., Itazaki M., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 66 - 66 2006.2

Analysis of AHL-production Defective Mutant, Δorf3 of Pseudomonas syringae pv. tabaci (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Taguchi F., Ogawa Y., Suzuki T., Takeuchi K., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 79 - 79 2006.2

HR-inducible Domain of Pseudomonas syringae pv. tabaci Flagellin in Arabidopsis thaliana (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Naito K., Ishiga Y., Taguchi F., Takeuchi K., Katoh E., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 78 - 79 2006.2

Signal Transduction through the Plant Cell Wall-plasma Membrane Continuum (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

Hirai Y., Toyoda K., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 72 ( 1 ) 66 - 66 2006.2

Induction of defense responses in pea tissues by inorganic phosphate.

Kawahara, T, Namba, H, Toyoda, K, Kasai, T, Sugimoto, M, Inagaki, Y, Ichinose, Y, Shiraishi, T

J. Gen. Plant Pathol. 72 ( 3 ) 129 - 136 2006

A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms

KIBA Akinori, TOYODA Kazuhiro, YOSHIOKA Kazuaki, TSUJIMURA Kami, TAKAHASHI Hirotaka, ICHINOSE Yuki, TAKEDA Tadahiro, KATO Toshiaki, SHIRAISHI Tomonori

J Gen Plant Pathol 72 ( 4 ) 238 - 246 2006

Peas extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen.

Kasai, T, Suzuki, T, Ono, K, Ogawa, K, Inagaki, Y, Ichinose, Y, Toyoda, K, Shiraishi, T

J. Gen. Plant Pathol. 72 ( 5 ) 265 - 272 2006

Taguchi Fumiko, Ogawa Yujiro, Takeuchi Kasumi, Suzuki Tomoko, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

Journal of Bacteriology 188 ( 24 ) 8376 - 8384 2006

A binding protein for fungal signal molecules in cell wall of Pisum sativum.

Kiba, A, Ohgawara, T, Toyoda, K, Inoue-Ozaki, M, Takeda, T, Rao, U.S, Kato, T, Ichinose, Y, Shiraishi, T

J. Gen. Plant Pathol. 72 ( 4 ) 228 - 237 2006

AtOPR3高発現シロイヌナズナを用いた植物病原菌相互作用におけるOPR subgroup 2の機能解析

松井英譲, 豊田和弘, 稲垣善茂, 白石友紀, 一瀬勇規

日本植物病理学会報 72 ( 4 ) 2006

(12) Esterase Activity of Extracellular Material Released from Powdery Mildew Conidia on Artificial Membrane and Plant Surfaces(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Suzuki T., Fujita K., Carver T. L., Thomas B., Toyoda K., Shiraishi T., Kunoh H.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 188 - 188 2005.8

(18) Rer1 Gene Homologues Expressed in Medicago truncatula and Barley Challenged with Virulent Pathogens(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Yoshihiro M., Hirose M., Fujita K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 190 - 190 2005.8

(10) Silencing of NbCdc27B Induces Defense-Related Gene Expression and Resistance to C. lagenarium(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Kudo C., Inagaki Y. S., Fukuoka S., Suzuki T., Takano Y., Okuno T., Asai S., Suenaga H., Sasabe M., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 187 - 188 2005.8

(17) Medicago truncatula Genes Expressed in Susceptible Interaction with Mycosphaerella pinodes; Potential Role of Cellulose Synthase Gene(s) in Achievement of Susceptibility to Fungal Pathogen(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Kohya N., Hirose M., Hata T., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 189 - 190 2005.8

(33) Differential Expression of Strawberry Genes Induced by Host-Specific AF-toxins Produced by the Strawberry Pathotype of Alternaria alternata (3) : Specific Gene Expression Induced by AF-toxin I and II(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Kitani K., Toyoda K., Shiraishi T., Yamamoto M.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 194 - 194 2005.8

(19) Functional Analysis of AtOPR1 and 2 in the Interactions with Fungal Pathogens using AtOPR1- and AtOPR2-Overexpressed Transgenic Arabidopsis(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Matsui H., Itazaki M., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 190 - 190 2005.8

(13) Pea Apoplastic Cu/Zn-SOD Responds to Pathogen Signal Molecules(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Kasai T., Suzuki T., Toyoda K., Inagaki Y., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 188 - 188 2005.8

(14) Plant Cell Wall as the First Line of Defense Involving Perception of Pathogen Signals and the Subsequent Transcriptional Response in Pea-Mycosphaerelas pinodes Interaction(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Hirai Y., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 188 - 189 2005.8

(16) Molecular and Genetic Dissection of Plant Susceptibility to Fungal Pathogen; Toward Isolation of Plant Susceptibility Gene(s) Required for the Achievement of Successful Infection and the Subsequent Pathogen Growth(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Hirose M., Maeda A., Iwahashi K., Ikeda S., Kohya N., Yoshihiro M., Matusuo K., Nagai C., Kawakami E., Hata T., Tanaka Y., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 189 - 189 2005.8

(11) Isolation of Insensitive Mutants to INF1 Elicitin and Expression Pattern of Defense Genes in the A04 Mutant(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Asai S., Inagaki Y. S., Kudo C., Ishikawa A., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 188 - 188 2005.8

(15) Modification of Plant Virulence Target Alters the Resistance and Susceptibility to Pathogens(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Tsujimura K., Kawahara T., Yoshioka K., Nagai C., Kiba K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 189 - 189 2005.8

(406) Orf3 Gene in the Glycosylation Island of Pseudomonas syringae pv. tabaci Regulates Expression of Virulence-Related Genes Via Quorum Sensing Molecules(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Ogawa Y., Taguchi F., Marutani M., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 294 - 294 2005.8

(430) Defense Responses of Arabidopsis thaliana Treated with PAMPs from Pseudomonas syringae(Abstracts of the Paper Presented at the 2005 Annual Meeting in Shizuoka)

Ishiga Y., Suenaga H., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 300 - 300 2005.8

(405) Functional Analysis of Gac Two-Component System in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

Marutani M., Ogawa Y., Taguchi F., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 3 ) 294 - 294 2005.8

(53) Protein Expression and Enzyme Activity of AFT8, a Gene Found in the AF-toxin Biosynthesis Gene Cluster of the Alternaria alternata Strawberry Pathotype(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Kitani K., Ruswandi S., Takahara H., Hosaki Y., Akimitsu K., Tsuge T., Toyoda K., Shiraishi T., Yamamoto M.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 66 - 66 2005.2

(4) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic and Molecular Dissection of Susceptibility to Fungal Pathogen. VI : A M. truncatula Suspension Cultured Cell Line with Responsiveness to Pathogen Signal Molecules and in vitro Regeneration(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Matsuo K., Tani C., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 53 - 53 2005.2

(57) Defense Responses of Arabidopsis thaliana Treated with Flagellin from Pseudomonas syringae pv. tabaci Wild Type and Oligopeptide flg22 (2)(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Ishiga Y., Lin Y., Taguchi F., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 67 - 67 2005.2

(2) Response to Fungal Pathogen in Transgenic Tobacco Plants Overexpressing Pea Apyrase Gene (PsAPY1)(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Tsujimura K., Kiba A., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 52 - 52 2005.2

(5) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic and Molecular Dissection of Susceptibility to Fungal pathogen VII : Production of M. truncatula Mutant Library and Screening of Mutants with Decreased Susceptibility(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Toyoda K., Hirose M., Maeda A., Koya N., Matsuo K., Yoshihiro M., Tanaka Y., Kawakami E., Nagai C., Inagaki Y., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 53 - 53 2005.2

(1) Establishment of Gene Disruption Experiment in Mycosphaerella pinodes(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Takahara H., Miyake Y., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 52 - 52 2005.2

(3) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic and Molecular Dissection of Susceptibility to Fungal Pathogen V. : Genes Expressed in M. truncatula Leaves during the Establishment of a Compatible Interaction with a Foliar Pathogen, M. pinodes(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Hirose M., Yoshihiro M., Hata T., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 52 - 53 2005.2

(43) Isolation of Insensitive Mutants to INF1 Elicitin in Nicotiana benthamiana(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Asai S., Ishikawa A., Kudo C., Toyoda K., Inagaki Y. S., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 63 - 63 2005.2

(42) Does APC Involve in INF1 Elicitin-Induced Hypersensitive Cell Death?(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Kudo C., Fukuoka S., Suenaga H., Sasabe M., Asai S., Toyoda K., Inagaki Y. S., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 71 ( 1 ) 63 - 63 2005.2

Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)

ISHIGA Y

J Gen Plant Pathol 71 ( 4 ) 302 - 307 2005

Flagellin from Pseudomonas syringae pv. tabaci induced hrp-independent HR in tomato

MARUTANI M

J Gen Plant Pathol 71 ( 4 ) 289 - 295 2005

Pea apoplastic Cu/Zn-SOD responsive to pathogenic signal molecules

T Kasai, K Ono, T Suzuki, K Toyoda, Y Inagaki, Y Ichinose, T Shiraishi

PLANT AND CELL PHYSIOLOGY 46 S94 - S94 2005

Mechanism of flagellin-induced hypersensitive cell death in Arabidopsis thaliana

Y Ishiga, YC Lin, M Marutani, F Taguchi, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

PLANT AND CELL PHYSIOLOGY 46 S94 - S94 2005

(370) Defense Responses of Arabidopsis thaliana Treated with Flagellin from Pseudomonas syringae pv. tabaci Wild Type and Oligopeptide flg22(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

Ishiga Y., Lin Y., Taguchi F., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 70 ( 3 ) 290 - 290 2004.8

(45) Response of Cowpea Apyrase Genes to Fungal Elicitor or Suppressor from M. pinodes(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

Takahashi H., Toyoda K., Hirakawa Y., Morishita K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 70 ( 3 ) 202 - 202 2004.8

(46) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic Dissection of Susceptibility to Fungal Pathogen IV. : Further Efforts to Set up the Model Pathosystem for a Better Understanding of Fungal Suppressor-mediated Diseases(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

Kawakami E., Kohya N., Hirose M., Nagai C., Matsuo K., Tani C., Hata T., Morikawa J., Inagaki Y., Yamamoto M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 70 ( 3 ) 202 - 202 2004.8

(64) Apyrase Is Not Required for INF1- and Avr9-induced Cell Death in Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

Nagai C., Yoshioka H., Inagaki Y., Yamamoto M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 70 ( 3 ) 207 - 207 2004.8

(74) Differential Expression of Strawberry Genes Induced by Host-specific AF-toxins Produced by the Strawberry Pathotype of Alternaria alternata : (2) Quantitative Time Course of Differentially Expressed Genes(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

Kitani K., Toyoda K., Shiraishi T., Yamamoto M.

Annals of the Phytopathological Society of Japan 70 ( 3 ) 210 - 210 2004.8

(44) Characterization of the Genes Expressed during Spore Germination of Mycosphaerella pinodes(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

Takahara H., Toyoda K., Tsuji G., Yamamoto M., Inagaki Y., Ichinose Y., Kubo Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 70 ( 3 ) 201 - 202 2004.8

(12) Establishment of Transformation System for Mycosphaerella pinodes to Isolate Virulence-Related Genes (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Miyake Y., Takahara H., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 70 ( 1 ) 59 - 59 2004.2

(11) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic Dissection of Susceptibility to Fungal Pathogen III. : Establishment of Bioassay System (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

Morikawa J., Ikeda S., Inagaki Y., Yamamoto M., Ichinose Y., Toyoda K., Shiraishi T.

Annals of the Phytopathological Society of Japan 70 ( 1 ) 58 - 59 2004.2

Differential regulation of MBP kinases by a glycoproptein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea

Uppalapati, SR, K Toyoda, Yasuhiro, I, Y Ichinose, T Shiraishi

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY 64 ( 1 ) 17 - 25 2004.1

Language：English Publisher：ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD

A polypeptide fungal suppressor from a pea pathogen Mycosphaerella pinodes plays a key role in pathogenesis by suppressing elicitor-induced defense response(s) in pea (Pisum sativum L). In this study, we show that treatment of pea tissues with the polysaccharide elicitor secreted by M. pinodes results in rapid increased activation of two myelin basic protein (MBP)-dependent kinases p44 (approximate to44 kDa) and p48 (approximate to48 kDa) within 15-30 min upon elicitation. Interestingly, the suppressor inhibited the elicitor-induced activation of only p44 kinase. While the defense-inducing signalling molecules, chitosan and salicylic acid (SA) activated the p44 and p48 kinases, methyl jasmonate (MeJA) did not. The abiotic stress signals, abscisic acid (ABA), NaCl and wounding activated the p48 kinase alone. These results demonstrate that MAPKs are differentially activated in response to pathogen invasion and abiotic stress in pea. Furthermore, specific inhibition of elicitor-induced p44 kinase activation by a MAPKK inhibitor, PD098059 and protein kinase inhibitor, K252a correlated with the suppression of elicitor-induced phenylalanine ammonia lyase (PAL) gene expression, supporting a role for p44 in the elicitor-induced defense response(s) in pea. Inhibition of p44 by the phosphoinositide (PI) turnover inhibitor, neomycin (a fungal suppressor mimic), and potentiation of p44 by the diacylglycerol (DAG) kinase inhibitor, R59022 indicated that p44 may be acting downstream of (PI) metabolism. Taken together, our results indicate that suppressor of defense elicitation from M. pinodes acts through inhibition of a MAPK (p44), possibly through a PI signaling pathway, facilitating the establishment of basic compatibility during infection of pea. (C) 2004 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.pmpp.2004.05.003

Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea

PDR-type ABC transporter and tobacco defense response against pathogen

Y Ichinose, D Schenke, M Sasabe, Y Inagaki, K Toyoda, T Shiraishi

PLANT AND CELL PHYSIOLOGY 45 S1 - S1 2004

Resistance in Pea Plants induced by Carpropamid and Related Compounds(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

Matsumoto M., Akisada S., Araki Y., Sawada H., Toyoda K., Yamamoto M., Inagaki Y., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 69 ( 3 ) 255 - 256 2003.8

A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model for Genetic Dissection of Susceptibility to Fungal Pathogen II. Achievement of Susceptibilty by Fungal Suppresicins(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

Ikeda S., Morikawa J., Toyoda K.

Annals of the Phytopathological Society of Japan 69 ( 3 ) 247 - 248 2003.8

The Role of Diamineoxidase in Plant Defense 2. Regulatory Mechanism on Its Activation(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

Iwahashi K., Ohwatari Y., Kawahara T., Toyoda K., Yamamoto M., Inagaki Y., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 69 ( 3 ) 250 - 250 2003.8

Analysis of Pea Apoplastic Cu/Zn-SOD Responsive to Pathogenic Signal Molecules(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

Kasai T., Ono K., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 69 ( 3 ) 250 - 250 2003.8

Characterization of Plasma Membrane Proteins Affinity-purified with Concanavalin A (Con A), a Potent Elicitor of Phytoalexin Accumulation(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

Okazaki A., Toyoda K., Sugimoto M., Inagaki Y., Yamamoto M., Ichinose Y., Shiraichi T.

Annals of the Phytopathological Society of Japan 69 ( 3 ) 249 - 250 2003.8

Construction of subracted cDNA Library of Strawberry Treated by AF-toxins from Alternaria alternata Strwberry Pathotype(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

Kitani K., Toyoda K., Shiraishi T., Yamamoto M.

Annals of the Phytopathological Society of Japan 69 ( 3 ) 252 - 253 2003.8

Flagellin-induced HR Cell Death Is Independent of Type III Secretion System in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

Marutani M., Taguchi F., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 69 ( 3 ) 300 - 301 2003.8

Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model for the Molecular and Genetic Dissection of Plant-microbe Interaction

Toyoda K., Ikeda S., Morikawa J., Inagaki Y., Ichinose Y., Yamamoto M., Shiraishi T.

Annals of the Phytopathological Society of Japan 69 ( 1 ) 56 - 56 2003.2

Endogenous Suppressor in Arabidopsis thaliana

Rao U.S., Matsushita H., Toyoda K., Ichinose Y., Inagaki Y., Yamamoto M., Tsuji G., Kubo Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 69 ( 1 ) 56 - 56 2003.2

Extracellular PO_4^- induces the expression of peroxidases in Pisum sativum

Kawahara T., Nanba H., Toyoda K., Inagaki Y., Ichinose Y., Yamamoto M., Shiraishi T.

Annals of the Phytopathological Society of Japan 69 ( 1 ) 56 - 57 2003.2

Characterization of elicitor-responsive receptor-like protein kinase in tobacco

Sasabe M., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 69 ( 1 ) 56 - 56 2003.2

Analysis of Suppressor-responsive Genes : (5) Expression of OPR Gene Family

Matsui H., Nakamura G., Ishiga Y., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 69 ( 1 ) 57 - 57 2003.2

Seki Hikaru, Marutani Mizuri, Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

92 ( 1 ) 21 - 26 2003.2

Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci : genetic analysis with flagella-defective mutants ΔfliC and ΔfliD in host tobacco plants

ICHINOSE Yuki, SHIMIZU Rena, IKEDA Yoko, TAGUCHI Fumiko, MARUTANI Mizuri, MUKAIHARA Takafumi, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori

J. Gen. Plant Pathol. 69 ( 4 ) 244 - 249 2003

Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens

ISHIGA Yasuhiro, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori, ICHINOSE Yuki

J Gen Plant Pathol 69 ( 6 ) 351 - 357 2003

Phylogenetic Classification of Dof- type Transcription Factors in Pea (Pisum sativum)

NAKAMURA Naoki, MARUTANI Mizuri, SANEMATSU Shiroh, TOYODA Kazuhiro, INAGAKI Yoshi-shige, SHIRAISHI Tomonori, ICHINOSE Yuki

Plant tissue culture letters 20 ( 3 ) 247 - 252 2003

Language：English Publisher：Japanese Society for Plant Cell and Molecular Biology

The Dof protein family is a group of plant transcription factors carrying highly conserved 52 residues referred to as the Dof domain, and belongs to the C4 zinc-finger transcription factors. We isolated various PsDof genes by PCR using the Dof-domain nucleotide sequence of the PsDof1 gene from a cDNA library of elicitor-treated pea epicotyls, and these isolated PsDof genes were then classified phylogenetically. Since the obtained genes (PsDof1 to PsDof7) were scattered over various positions of the phylogenetic tree, they were expected to perform various functions as Dof-type transcription factors. From their positions in the tree, it is expected that the PsDof2 and PsDof5 genes are defense related, as is the PsDof1 gene.

DOI： 10.5511/plantbiotechnology.20.247

Flagella-mediated Interactions between Phytopathogenic Bacteria and Plants

Other Link： https://jlc.jst.go.jp/DN/JALC/00231800981?from=CiNii

ICHINOSE Yuki, TAGUCHI Fumiko, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori

41 ( 8 ) 511 - 516 2003

Post-translational modification of flagellin determines the specificity of HR induction

TAGUCHI F

Plant Cell Physiol 44 ( 3 ) 342 - 349 2003

Cloning and characterization of pea apyrases: Involvent of psAPY1 in response to signal molecules from the pea pathogen, Mycosphaerella pinodes

Journal of plant pathology 69, 33-38 2003

Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

TAGUCHI F

Plant Physiol Biochem 41 165 - 174 2003

Role of flagella and flagellin in plant - Pseudomonas syringae interactions

Y Ichinose, R Shimizu, F Taguchi, K Takeuchi, M Marutani, T Mukaihara, Y Inagaki, K Toyoda, T Shiraishi

PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS pp. 311-318. 311 - 318 2003

Language：English Publisher：SPRINGER

The role of flagella and monomer flagellin of Pseudomonas syringae pv. tabaci in plant-bacteria interactions was investigated by using non-polar fliC or fliD mutants. These mutants deleted the open reading frames for fliC and fliD, respectively, and both mutants lost all flagella and motility. The DeltafliC mutant did not produce flagellin, whereas DeltafliD mutant, that lost HAP2 protein, secreted a large amount of monomer flagellin in the culture medium. Inoculation of tomato leaves with wild type and DeltafliD mutant of P. s. pv. tabaci induced HR, whereas the DeltafliC mutant caused symptom-like change and propagated as P. s. pv. tomato. In tomato suspension cultured cells, wild type P. s. pv. tabaci induced visible HR-like changes. The DeltafliC mutant did not induce the HR, but the response was activated by the DeltafliD mutant. The expression of typical defence genes such as PAL and hsr203J was rapidly and strongly induced by inoculation with the DeltafliD mutant compared to inoculation with wild type P. s. pv. tabaci. On the other hand, both fliC and fliD mutants were reduced in virulence when inoculated into host tobacco leaves. Furthermore, complementation of fliC gene in DeltafliC mutant restored motility and HR-inducing ability in tomato, and virulence in tobacco. These results suggest that the monomer flagellin of P. s. pv. tabaci is an essential factor in the elicitation of HR in non host tomato cells, and flagella are required for complete virulence in host tobacco cells.

Resistance and susceptibility of plants to fungal pathogens

K Toyoda, NC Collins, A Takahashi, K Shirasu

TRANSGENIC RESEARCH 11 ( 6 ) 567 - 582 2002.12

Language：English Publishing type：Book review, literature introduction, etc. Publisher：KLUWER ACADEMIC PUBL

Plants are under continuous threat of infection by pathogens endowed with diverse strategies to colonize their host. Comprehensive biochemical and genetic approaches are now starting to reveal the complex signaling pathways that mediate plant disease resistance. Initiation of defense signaling often involves specific recognition of invading pathogens by the products of specialized host resistance (R) genes. Potential resistance signaling components have been identified by mutational analyses to be required for specific resistance in the model Arabidopsis and some crop species. Strikingly, many of the components share similarity to that of innate immune systems in animals. Evidence is also accumulating that plant pathogens have a number of ways to evade host defenses during the early stages of infection, similar to animal pathogens. These strategies are becoming much better understood in a number of plant- pathogen interactions. In this review, we focus on the current knowledge of host factors that control plant resistance and susceptibility to fungal pathogens. The knowledge accumulated in these studies will serve a fundamental basis for combating diseases in strategic molecular agriculture.

DOI： 10.1023/A:1021182111770

Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen

Y Ishiga, A Funato, T Tachiki, K Toyoda, T Shiraishi, T Yamada, Y Ichinose

PLANT AND CELL PHYSIOLOGY 43 ( 10 ) 1210 - 1220 2002.10

Language：English Publisher：OXFORD UNIV PRESS

Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressor-inducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabiei, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.

DOI： 10.1093/pcp/pcf144

Pseudomonas syringae pv. tabaciの単量体Flagellinは,非宿主植物トマト細胞にHR誘導因子として働く

(44)Plant Recognition and Response to Pathogens-Response of Non-host Plant's NTPase to Pathogen Signals

Takahashi H., Hirakawa Y., Morishita K., Kawahara T., Yoshioka K., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 68 ( 2 ) 168 - 168 2002.8

(43)Response of Cu/Zn-SOD in Pea Apoplast to Pathogen Signals

Kasai T., Ono K., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 68 ( 2 ) 167 - 168 2002.8

Hypersensitive Response-Inducing Factors Produced by Pseudomonas syringae (4) Functional Analysis of Flagellin Using Flagella-Deficient Mutants(Abstracts Presented at the Meeting of the Kansai Division)

Shimizu R, Taguchi F, Ikeda Y, Toyoda K, Shiraishi T, Inagaki Y, Ichinose Y

Annals of the Phytopathological Society of Japan 68 ( 1 ) 100 - 100 2002.4

Analysis of Elicitin-Responsive Genes in Tobacco BY-2 Cells, ATP-Binding Cassette Transporter Gene is Induced by INF1 Elicitin(Abstracts Presented at the Meeting of the Kansai Division)

Sasabe M., Toyoda K., Shiraishi T., Inagaki Y., Ichinose Y.

Annals of the Phytopathological Society of Japan 68 ( 1 ) 92 - 93 2002.4

清水鈴菜, 田口富美子, 池田陽子, 豊田和弘, 白石友紀, 稲垣善茂, 一瀬勇規

日本植物生理学会年会要旨集 42nd 238 2002.3

高等植物における病害抵抗性シグナル伝達と病原菌による制御

日本植物病理学会、植物微生物相互作用研究の現状と将来展望 67 - 76 2002

Cloning and characterization of pea apyrases: Involvent of psAPY1 in response to signal molecules from the pea pathogen, Mycosphaerella pinodes

Journal of plant pathology 69, 33-38 2002

Cell wall-plasma membrane continuum is involved in transduction of pathogen signals

K Toyoda, AM Kiba, T Kawahara, M Sugimoto, RS Uppalapati, Y Inagaki, Y Ichinose, M Yamamoto, T Shiraishi

PLANT AND CELL PHYSIOLOGY 43 S10 - S10 2002

Flagellin, a constituent of a flagella filament in Pseudomonas syringae pv. tabaci, is an intense HR elicitor as a monomer molecule on nonhost tomato cells

R Shimizu, F Taguchi, Y Ikeda, K Toyoda, T Shiraishi, Y Inagaki, Y Ichinose

PLANT AND CELL PHYSIOLOGY 43 S201 - S201 2002

Gramine increase associated with rapid and transient systemic resistance in barley seedlings induced by mechanical and biological stresses

H Matsuo, K Taniguchi, T Hiramoto, T Yamada, Y Ichinose, K Toyoda, K Takeda, T Shiraishi

PLANT AND CELL PHYSIOLOGY 42 ( 10 ) 1103 - 1111 2001.10

Language：English Publisher：OXFORD UNIV PRESS

Systemic acquired resistance (SAR) is one of the intriguing issues for studying the mechanism in signal transduction system in a whole plant. We found that SAR and increase of an antifungal compound were induced rapidly and transiently in barley (Hordeum vulgare L. cv. Goseshikoku) by mechanical and biological stresses. One of the major antifungal compounds was identified as an indole alkaloid, gramine (N,N-dimethyl-3-aminomethylindole), by mass spectrum and NMR analyses. Gramine is well known as a constitutive compound of barley, but it increased significantly in the primary and secondary leaves of barley seedlings within 12 h after pruning or inoculating with the powdery mildew fungi of barley (Blumeria graminis Esp. hordei) and wheat (B. graminis Esp. tritici). However, in the leaf detached from unwounded seedlings or in the leaf inoculated with the barley powdery mildew fungus, gramine did not increase at all. In the water droplets contacted with barley leaves, the amount of leaked gramine increased dependently upon the time after the seedling was injured mechanically. We also found a tight correlation between gramine increase and enhancement of resistance to the barley powdery mildew fungus in barley leaves treated with an endogenous elicitor. Furthermore, such a systemic resistance was not observed in a barley cultivar Morex that lacks the biosynthetic pathway of gramine. From these results, we conclude that gramine is the excellent marker in rapid and transient systemic acquired resistance in barley.

DOI： 10.1093/pcp/pce139

Generation of hydrogen peroxide is not required for harpin-induced apoptotic cell death in tobacco BY-2 cell suspension culture

Y Ichinose, S Andi, R Doi, R Tanaka, F Taguchi, M Sasabe, K Toyoda, T Shiraishi, T Yamada

PLANT PHYSIOLOGY AND BIOCHEMISTRY 39 ( 9 ) 771 - 776 2001.9

Language：English Publisher：GAUTHIER-VILLARS/EDITIONS ELSEVIER

To characterize molecular and biochemical mechanisms of hypersensitive reaction (HR) in plants, a tobacco suspension culture of BY-2 was treated with the proteinaceous HR elicitor harpin from several pathovars of Pseudomonas syringae. Tobacco BY-2 cells are sensitive to harpins derived from non-pathogenic pathovars of P. syringae, such as pvs. pisi, tomato and glycinea. These three harpins induce apoptotic cell death accompanied by DNA fragmentation in BY-2. Because the cell death was also accompanied by rapid generation of hydrogen peroxide (H2O2), one of the active oxygen species, we investigated the role of H2O2 in harpin-induced cell death. Although treatment with diphenylene iodium chloride (DPI) reduced, and catalase completely abolished, the harpin-induced generation of H2O2, the frequency of cell death was not affected at all. Treatment with superoxide dismutase (SOD) enhanced the generation of H2O2, but the cell death was unaffected. These results indicate that harpin-induced apoptotic cell death does not require oxidative burst. (C) 2001 Editions scientifiques et medicales Elsevier SAS

DOI： 10.1016/S0981-9428(01)01300-6

Effect of Harpin from Four Pathovars of Pseudomonas syringae on Pea Defense Responses (Short Communication) :

(325)Effect of Methyl Jasmonate on Harpin-Induced Hypersensitive Reaction in Tobacco

Andi S., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y

Annals of the Phytopathological Society of Japan 67 ( 2 ) 205 - 205 2001.8

Molecular Cloning and Bacterial Expression of Extracellular Peroxidase from Pisum sativum : The Role in Superoxide Generation

Nanba H., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 120 - 120 2001.8

Characterization of Cell Wall Proteins that Interact with Plasma Membrane

Ozaki H., Yuasa Y., Kiba A., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 120 - 121 2001.8

Characterization of a Membrane-associated Protein Kinase that Phosphorylates Phosphatidylinositol-4-phosphate 5-kinase(PIP5K)

Okazaki A., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 121 - 121 2001.8

Plant Recognition and Response to Pathogens(2). Cloning of NTPase Genes from Host and Non-host plants

Takahashi H., Kawahara T., Miura A., Yoshioka A., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 120 - 120 2001.8

Use of Oxygen Meter to Detect Initial Response Induced in Strawberry Leaves by AF-toxin

Yamamoto M., Toyoda K., Ichinose Y., Shiraishi T., Arai J.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 123 - 123 2001.8

Molecular Characterization of Dof DNA Binding Protein in Pea

Nakamura M., Marutani M., Seki H., Toyoda K., Shiraishi T, Ichinose Y.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 206 - 206 2001.8

Oxidoredox Enzymes and Recognition/Response to Pathogens in Plant Cell Wall : Ascorbate Oxidase

Osaki O., Kiba A., Toyoda K., Yamamoto M., Ichinose Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 120 - 120 2001.8

Hypersensitive Reaction-Inducing Factors Produced in Pseudomonas syringae(4)Structural and Functional Analysis of Flagellin

Shimizu R., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

Annals of the Phytopathological Society of Japan 67 ( 2 ) 205 - 206 2001.8

TANAKA Rui, TAGUCHI Fumiko, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general plant pathology : JGPP 67 ( 2 ) 148 - 151 2001

Language：English Publisher：Phytopathological Society of Japan

To investigate the role of the proteinaceous elicitor, harpin, on host and nonhost plants, we isolated the harpin-coding gene, hrpZ, from Pseudomonas syringae pvs. pisi, glycinea, tabaci and tomato. Effects of the recombinant harpin proteins on pea plants were analyzed and compared with the effects of the corresponding bacterial treatment. After inoculation of pea with pea pathogen P. syringae pv. pisi, the bacterial population increased and the accumulation of PAL-mRNA and pisatin was inhibited. The nonpathogenic pathovars, glycinea, tabaci and tomato induced both defense responses in pea. However, none of the harpins induced the hypersensitive reaction or accumulation of PAL-mRNA and pisatin in pea. Harpins from P. syringae pvs. glycinea, tomato and pisi did induce these defense responses in tobacco, however, suggesting that externally applied harpins either are not recognized or are nonfunctional in pea plants. (Received July 27, 2000 ; Accepted February 21, 2001)

DOI： 10.1007/PL00013001

Other Link： http://id.nii.ac.jp/1141/00138226/

Effect of methyl jasmonate on harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of defense genes in tobacco suspension cultured BY-2 cells.

Andi, S, Taguchi, F, Toyoda, K, Shiraishi, T, Ichinose, Y

Plant Cell Physiol. 42 ( 4 ) 446 - 449 2001

Harpin_<psta> from Pseudomonas syringae pv. tabaci is defective and deficient in its expression and HR-inducing activity.

TAGUCHI F.

J. Gen. Plant Pathol. 67 ( 2 ) 116 - 123 2001

初期・表層シグナル伝達系と防御システム

白石友紀, 豊田和弘, 木場章範, 一瀬勇規

化学と生物 39 (10): 686-692 ( 10 ) 686 - 692 2001

The Role of Active Oxygen Species Accompanied with HR by Elicitin in Tobacco Cell Suspension Culture(Abstracts Presented at the Meeting of the Kansai Division)

Takeuchi K., Sasabe M., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 287 - 288 2000.12

Hypersensitive Response-inducing Factors Produced in Pseudomonas syringae : (2) Induction and Suppression of Hypersensitive Reponse by Harpin(Abstracts Presented at the Meeting of the Kansai Division)

Andi S., Taguchi F., Doi R., Tanaka R., Sasabe M., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 292 - 293 2000.12

Cell Wall NTPase in Pea Recognizes the Signal Molecules from Mycosphaerella pinodes(Abstracts Presented at the Meeting of the Kansai Division)

Yoshioka K., Kiba A., Kawahara T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 288 - 288 2000.12

Hypersensitive Response-inducing Factors Produced in Pseudomonas syringae : (3) Isolation and Functional Analysis of Flagellin as an Elicitor Molecule(Abstracts Presented at the Meeting of the Kansai Division)

Taguchi F., Nakajima R., Andi s., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 293 - 293 2000.12

Analysis of Suppressorresponsive Genes in Pea, : (3)Identification of S64 Protein as Oxophytodienoic Acid Reductase(Abstracts Presented at the Meeting of the Kansai Division)

Ishiga Y., Ichinose Y., Toyoda K., Shiraisi T., Yamada T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 288 - 288 2000.12

Interaction between an Integrin-like Protein(s) and a Phosphatidylinositol 4-Phosphate 5-Kinase (PIP5K) in Pea Plasma Membrane(Abstracts Presented at the Meeting of the Kansai Division)

Toyoda K., Okazaki A., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 288 - 288 2000.12

Hypersensitive Response-Inducing Factors Produced in Pseudomonas syringae : (1) Structural and Functional Analysis of Harpin Pseudomonas syringae(Abstracts Presented at the Meeting of the Kansai Division)

Tanaka R., Taguchi F., Andi S., Kinoshita S., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

Annals of the Phytopathological Society of Japan 66 ( 3 ) 292 - 292 2000.12

Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

JOURNAL OF PHYTOPATHOLOGY 148 ( 11-12 ) 633 - 636 2000.12

Language：English Publisher：WILEY-BLACKWELL

When epicotyl tissues of pea were treated with a diacylglycerol (DAG) kinase inhibitor (R59022), enhanced induction of the phytoalexin accumulation occurred which was induced by fungal elicitor. The marked induction was associated with a sustained accumulation of phenylalanine ammonia-lyase (PAL)-mRNA and the consequent increase in PAL activity. These results suggest that inhibition of DAG breakdown leads to increased induction of phytoalexin accumulation and support the hypothesis that DAG kinase negatively regulates the signal transduction.

DOI： 10.1046/j.1439-0434.2000.00568.x

Specific Inhibition of Cell Wall-bound ATPase by the Suppressor from Mycosphaerella pinodes(6) : Cloning and Functional Analysis of Fungal Signal-binding Protein from Pea Plant

Kiba A., Ohgawara T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 66 ( 2 ) 116 - 117 2000.8

Cytochalasin A inhibits the binding of phenylalanine ammonia-lyase mRNA to ribosomes during induction of phytoalexin in pea seedlings

M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 41 ( 2 ) 234 - 238 2000.2

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Cytochalasin A (CA) blocked the accumulation of phytoalexin and phenylalanine ammonia-lyase (PAL)-protein in pea tissues treated with a fungal elicitor but scarcely affected the PAL-mRNA content. Further analysis showed that CA decreased the PAL-mRNA bound to ribosomes. These results indicate that actin filaments are tightly associated with the translational process of the PAL gene.

Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization

Y Ichinose, K Tiemann, C Schwenger-Erger, K Toyoda, F Hein, T Hanselle, H Cornels, W Barz

ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES 55 ( 1-2 ) 44 - 54 2000.1

Language：English Publisher：VERLAG Z NATURFORSCH

In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanisms at the level of gene activation and deactivation, differential screenings were performed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism. Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNAs provide a variety of tools to investigate molecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

Importance of AC-rich element on pea phenylalanine ammonia-lyase gene 1 promoter for expression induced by nonpathogenic attack

IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

Journal of general Plant Pathology 66 ( 2 ) 123 - 127 2000

Language：English Publisher：Phytopathological Society of Japan

Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

DOI： 10.1007/PL00012932

PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS :

Other Link： http://id.nii.ac.jp/1141/00138133/

KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Plant and cell physiology 41 s10 2000

INDEPENDENT PATHWAYS OF DEFENSE RESPONSES IN ELICITIN-TREATED TOBACCO BY-2 CELLS :

SASABE Michiko, TAKEUCHI Kasumi, KAMOUN Sophien, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology 41 s85 2000

STRUCTURAL AND FUNCTIONAL ANALYSES OF HARPINS FROM SEVERAL PATHOVARS OF PSEUDOMONAS SYRINGAE :

TANAKA Rui, TAGUCHI Tomiko, KINOSHITA Sayuri, NAKADA Hideki, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

Plant and cell physiology 41 s84 2000

Analysis of Suppressorresponsive Genes in Pea (2) : Molecular Structure and Gene Expression of S64 (Abstracts Presented at the Meeting of the Kansai Division)

Funato A., Ishiga Y., Ichinose Y., Toyoda K., Shiraishi T., Yamada T., Barz W.

Annals of the Phytopathological Society of Japan 65 ( 6 ) 671 - 671 1999.12

Induction of Resistance against Powdery Mildew in Barley Leaves by Volatiles from Tomato Seedlings (Abstracts Presented at the Meeting of the Kansai Division)

Watanabe T., Takabayashi J., Hiramoto T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 65 ( 6 ) 671 - 672 1999.12

Fungal Signals and Their Perception in Plant Cell Walls : Regulation of Diamine Oxidase Activity in Isolated Cell Wall from Pea Seedlings by an Elicitor and a Suppressor from Mycosphaerella pinodes (Abstracts Presented at the Meeting of the Kansai Division)

Ohwatari Y., Watanabe Y., Toyoda K., Ichinose Y., Yamada Y., Shiraishi T.

Annals of the Phytopathological Society of Japan 65 ( 6 ) 671 - 671 1999.12

Induction of Hypersensitive Cell Death and Defense Gene Expression by Elicitin in Tobacco Cell Suspension Culture (Abstracts Presented at the Meeting of the Kansai Division)

Sasabe M., Takeuchi K., Kamoun S., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 65 ( 6 ) 671 - 671 1999.12

cDNA cloning and gene expression of three small GTP-binding proteins in defense response of chickpea

Y Ichinose, K Toyoda, W Barz

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1489 ( 2-3 ) 462 - 466 1999.12

Language：English Publisher：ELSEVIER SCIENCE BV

The cDNA clones encoding rab type (INR134 and ELR19) and rac type (ELR26) small GTP-binding proteins were isolated from Ascochyta rabiei-inoculated chickpea leaves and the elicitor-treated cell cultures. Rac type ELR26 showed enhanced expression in inoculated leaves indicating correlation with the defense response. (C) 1999 Elsevier Science B.V. All rights reserved.

DOI： 10.1016/S0167-4781(99)00201-8

Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

A Kiba, T Takeda, T Kanemitsu, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 40 ( 9 ) 978 - 985 1999.9

Language：English Publisher：OXFORD UNIV PRESS

A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man-O-Ser (No. 1) to [beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man](3)-O-Ser(3)-Pro(3) (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycopeptides [beta-D-Glc-(1-->6)-alpha-D-Man-(1-->6)-D-Man]-O-Ser(2)-Pro(2) (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

Isolation and Structural Analysis of Elicitor-responsive Gene in Pea : (4) : Analysis of hsr203J Homolog in Pea

Hisayasu Y., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 65 ( 3 ) 325 - 325 1999.6

Functional Analysis of Cis-regulatory Elements in PSPAL1 and Application to Molecular Breeding of Anti-bacterial Plants

Imura Y., Fujiwara T., Ozawa H., Tanaka H., Iguchi S., Toyoda K., Ichinose Y., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 65 ( 3 ) 372 - 373 1999.6

Analysis of Dof DNA Binding Protein Which was Induced by Wounding and Elicitor in Pea

Seki H., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 65 ( 3 ) 325 - 325 1999.6

Involvement of Actin Filaments in Defense Response of Pea

Sugimoto M., Toyoda K., Kiba A., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 65 ( 3 ) 325 - 325 1999.6

Integrin-like Protein(s) May Interact with Polyphosphoinositide Metabolism during Induction of Pea Defense Response

Toyoda K., Kiba A., Sugimoto M., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 65 ( 3 ) 324 - 325 1999.6

Co-purification of plasma membrare ATPase and phosphatidylinositol kinase from pea plasma membrane

Scientific Reports of the Faculty of Agriculture Okayama University 88 31 - 38 1999

Plant pathogens and mechanisms of defense responses

T. Yamada, T. Shiraishi, Y. Ichinose, K. Toyoda

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 44 ( 15 ) 2308 - 2314 1999

(32) Specific Inhibition of Cell Wall-bound ATPase by the Suppressor from Mycosphaerella pinodes (6) Elicitor- and Suppressor-binding Proteins in ATPase Fraction from Cowpea Cell Wall (Abstracts Presented at the Meeting of the Kansai Division, 1998)

Yamashita H., Kiba A., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 64 ( 6 ) 623 - 623 1998.12

(13) Induction of Superoxide-scavenging Enzyme-mRNAs in Fungal Elicitor-treated Cells of Chickpea (Cicer arietinum) (Abstracts Presented at the Meeting of the Kansai Division, 1998)

Toyoda K., Ichinose Y., Shiraishi T., Yamada T., Barz W.

Annals of the Phytopathological Society of Japan 64 ( 6 ) 618 - 618 1998.12

(8) Analysis of Signal Transmission between Cell Wall and Plasma Membrane (2) Interaction between Cell Wall and Plasma Membrane Is Implicated in Defense Responses of Pea Plant (Abstracts Presented at the Meeting of the Kansai Division, 1998)

Kiba A., Sugimoto M., Azuma T., Yuasa Y., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 64 ( 6 ) 617 - 617 1998.12

(9) Identification of Cis-regulatory Elements Involved in the Defense-associated and Organ-specific Expression of Pea PAL Genes by in vivo Footprinting Analysis (Abstracts Presented at the Meeting of the Kansai Division, 1998)

Imura Y., Seki H., Toyoda K., Ichinose Y., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 64 ( 6 ) 617 - 617 1998.12

(12) Characterization of Genes Expressed in Ascochyta rabiei-inoculated Chickpea Plants and Elicited Cell Cultures (Abstracts Presented at the Meeting of the Kansai Division, 1998)

Ichinose Y., Tiemann K., Schwenger-Erger C., Toyoda K., Hein F., Yamada T., Shiraishi T., Barz W.

Annals of the Phytopathological Society of Japan 64 ( 6 ) 618 - 618 1998.12

(7) Involvement of Actin Filaments in Defense Response of Pea (Abstracts Presented at the Meeting of the Kansai Division, 1998)

Sugimoto M., Toyoda K., Kiba A., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 64 ( 6 ) 616 - 617 1998.12

Analysis of a gene encoding D of DNA binding protein which was induced by wounding and elicitor in pea.

SEKI H., ICHINOSE Y., OKABE T., SANEMATSU S., TOYODA K., SHIRAISHI T., YAMADA T.

21 433 - 433 1998.12

Interaction between cell wall and plasma membrane via RGD motif is implicated in plant defense responses

A Kiba, M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT AND CELL PHYSIOLOGY 39 ( 11 ) 1245 - 1249 1998.11

Language：English Publisher：OXFORD UNIV PRESS

Integrin- and vitronectin-like proteins were found to exist in the pea plasma membrane and cell wall, respectively. The hexapeptide GRGDSP but not GRGESP inhibited either the binding of cell wall protein(s) to plasma membrane protein(s) or the defense response. A possible role of linkage via RGD motif in plant defenses is discussed.

Phosphorylation of Phosphatidylinositols and Production of Ｌｙsophospholipid in Pea Plasma Membrane Are Coordinately Regulated by Elicitor and Suppressor from Mycosphaerella pinodes

(123) Isolation and Structural Analysis of Elicitor-responsive Genes in Pea (3) Characterization for a Novel Putative Transcription Factor Possessing Dof Domain

Okabe T., Ichinose Y., Seki H., Toyoda K., Shiraishi T., Yamada T.

Annals of the Phytopathological Society of Japan 64 ( 4 ) 357 - 358 1998.8

(122) Specific Inhibition of Cell Wall-bound ATPase by the Suppressor from Mycosphaerella Pinodes (5) Analysis of Elicitor- and Suppressor-binding Proteins in Pea Cell Wall Fraction

Kiba A., Sugimoto M., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

Annals of the Phytopathological Society of Japan 64 ( 4 ) 357 - 357 1998.8

In vivo FOOTPRINTING ANALYSIS OF cis-REGULATORY ELEMENTS INVOLVED IN THE ELICITOR-MEDIATED ACTIVATION AND SUPPRESSOR-MEDIATED DEACTIVATION OF PEA PAL AND CHS

SEKI Hikaru, ICHINOSE Yuki, NAGASUGI Yumi, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

39 S43 - S143 1998.5

ISOLATION AND CHARACTERIZATION OF ELICITOR-RESPONSIVE GENES IN PEA

ICHINOSE Yuki, SANEMATSU Shiroh, ENDOH Ai, OKABE Takeharu, HISAYASU Yumiko, SEKI Hikaru, SHIRAISHI Tomonori, TOYODA Kazuhiro, YAMADA Tetsuji

39 S143 - S143 1998.5

PURIFICATION AND ANALYSIS OF ELICITOR BINDING-PROTEIN IN CELL WALL FRACTION FROM PEA

SUGIMOTO Megumi, KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

39 S143 - S143 1998.5

SPECIFIC REGULATION OF CELL WALL-BOUND ATPASE BY THE SUPPRESSOR FROM MYCOSPHAERELLA PINODES(3) -IDENTIFICATION OF ELICITOR- AND SUPPRESSOR-BINDING PROTEINS IN CELL WALL-BOUND ATPASE FRACTION FROM PEA PLANT-

KIBA Akinori, SUGIMOTO Megumi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

39 S144 - S144 1998.5

RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS II

SUGIURA Tetsuya, KIBA Akinori, AOYAGI Masaaki, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

39 S144 - S144 1998.5

Toyoda Kazuhiro, Koyama Masashi, Mizukoshi Rumi, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

87 ( 1 ) 109 - 116 1998.2

Plant cell wall my recognize pathogenic microorganisms

Tomonori SHIRAISHI, Akinori KIBA, Kazuhiro TOYODA, Yuki ICHINOSE, Tetsuji YAMADA

KASEAA 36 ( 4 ) 226 - 233 1998

Molecular cloning of a cDNA encoding a putative DNA-binding zinc-finger protein in pea

Scientific Reports of the Faculty of Agriculture Okayama University 88 25 - 30 1998

Elevation of diacylglycerol during the early stage of elicitor-signal transduction in Pea(Pisum sativum)

Kazuhiro TOYODA, Tomoharu KAWAHARA, Rumi MIZUKOSHI, Masashi KOYAMA, Yuki ICHINOSE, Tetsuji YAMADA, Tomonori SHIRAISHI

Annals of the phytopathological Society of Japan 64 ( 5 ) 485 - 487 1998

(9) Analysis of Suppressor-responsive Genes in Pea : (1) Isolation of the cDNAs by Differential Hybridization

TACHIKI T., ICHINOSE Y., TOYODA K., SHIRAISHI T., YAMADA T.

Annals of the Phytopathological Society of Japan 63 ( 6 ) 509 - 509 1997.12

(8) Isolation and Structural Analysis of Elicitor-responsive Genes in Pea (2)

ICHINOSE Y., SANEMATSU S., ENDOH A., OKABE T., HISAYASU Y., SEKI H., TOYODA K., SHIRAISHI T., YAMADA T.

Annals of the Phytopathological Society of Japan 63 ( 6 ) 508 - 509 1997.12

(7) Isolation and Structural Analysis of Elicitor-responsive Genes in Pea (1)

SANEMATSU S., ICHINOSE Y., ENDOH A., TOYODA K., SHIRAISHI T., YAMADA T.

Annals of the Phytopathological Society of Japan 63 ( 6 ) 508 - 508 1997.12

(6) Specific Inhibition of Cell Wrall-bound ATPase by the Suppressor from Mycospharella pinodes. : (IV) Identincation of Elicitor- and Suppressor-binding Proteins in Cell Wrall-bound ATPase-rich Fraction from Pea Plants

KIBA A., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 63 ( 6 ) 508 - 508 1997.12

(69) Nucleotide Sequence of the Coat Protein Gene of Tobamoviruses Isolated from Capsicum spp. Containing L3 Gene

HASHIMURA K., TAKEUCHI S., MIZUMOTO H., HAMADA H., OKUMURA A., KURODA T., TOYODA K., SUZUKI K., HIKICHI Y., OKUNO T.

Annals of the Phytopathological Society of Japan 63 ( 6 ) 523 - 524 1997.12

特異性決定に関わる分子スイッチ

白石友紀, 木場章範, 豊田和弘, 一瀬勇規, 山田哲治

日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan 61 71 - 71 1997.9

Con A-binding protein in plasma membranes may participate in signal-transduction cascades that lead to defense responses in pea.

K Toyoda, M Sugimoto, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

PLANT PHYSIOLOGY 114 ( 3 ) 1502 - 1502 1997.7

Specific regulation of cell wall-bound ATPase and peroxidase by the suppressor for defense response.

A Kiba, A Inata, C Miyake, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

PLANT PHYSIOLOGY 114 ( 3 ) 1176 - 1176 1997.7

Mechanisms of the planet disease resistance : Focusing on the suppressor

YAMADA Tetsuji, SHIRAISHI Tomonori, ICHINOSE Yuki, TOYODA Kazuhiro

Chemical regulation of plants 32 ( 1 ) 49 - 59 1997.6

(269) Infection Route of CMV in Gentian : 6) Spread of CMV Infection in Fields(Abstracts of the Papers Presented at the Annual Meeting of the Society)

SUZUKI K., OKUMURA A., NASU Y., TOYODA K., HIKICHI Y.

Annals of the Phytopathological Society of Japan 63 ( 3 ) 258 - 258 1997.6

(204) Synchronized Multiplication of Pseudomonas cichorii and Protein Synthesis in Lettuce Leaf(Abstracts of the Papers Presented at the Annual Meeting of the Society)

HIKICHI Y., HORIKOSHI M., HIROOKA T., TOYODA K., SUZUKI K., OKUNO T.

Annals of the Phytopathological Society of Japan 63 ( 3 ) 242 - 242 1997.6

(207) Behavior of Bioluminescent Ralstonia solanacearum Transformed by luxCDABE in Bacterial Wilt Resistant Plants(Abstracts of the Papers Presented at the Annual Meeting of the Society)

NASU Y., HORIKOSHI M., HIROOKA T., TOYODA K., SUZUKI K., OKUNO T., HIKICHI Y.

Annals of the Phytopathological Society of Japan 63 ( 3 ) 242 - 243 1997.6

(271) Epidemiological Studies on the Japanese Tobamovirus Strains Infecting the L^2-resistance Genotypes of Pepper, a Member of Pepper Mild Mottle Virus (PMMV) Sub-group : II. Are Infected Seeds the Initial Sources of Viral Infection?(Abstracts of the Papers Presented at the Annual Meeting of the Society)

TOYODA K., OKUMURA A., NASU Y., HIKICHI Y., OKUNO T., SUZUKI K.

Annals of the Phytopathological Society of Japan 63 ( 3 ) 258 - 259 1997.6

Orthovanadate Induces Phytoalexin Production in Pea Suspension-Cultured Cells

Kawahara Tomoharu, Toyoda Kazuhiro, Kiba Akinori, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

Scientific reports of the Faculty of Agriculture, Okayama University 86 33 - 41 1997.2

Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules.(共著)

KIBA A, MIYAKE C, TOYODA K, ICHINOSE Y, YAMADA T, SHIRAISHI T

Phytopathology 87 ( 8 ) 846 - 852 1997

The role of suppressors in determining host-parasite specificities in plant cells

T Shiraishi, T Yamada, Y Ichinose, A Kiba, K Toyoda

INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 172 172 55 - 93 1997

Nucleotide Sequence of the Coat Protein Gene of Pepper Mild

TOYODA K., OKUMURA A., NASU Y., HIKICHI Y., SUZUKI K.

Annals of the Phytopathological Society of Japan 62 ( 6 ) 598 - 598 1996.12

Bioluminescence of Pseudomonas solanacearum Transformed with luxCDABEOperon in Tomato Plants

NASU Y., HIKICHI Y., HORIKOSHI M., OKUMURA A., TOYODA K., HIROOKA T., SUZUKI K.

Annals of the Phytopathological Society of Japan 62 ( 6 ) 605 - 605 1996.12

Relationship between Multiplication of Pseudomonas cichorii in Vascular Bundle of Lettuce Leaves and Symptom Development of Bacterial Rot of Lettuce

HIKICHI Y., NASU Y., HORIKOSHI M., OKUMURA A., TOYODA K., HIROOKA T., SUZUKI K.

Annals of the Phytopathological Society of Japan 62 ( 6 ) 605 - 605 1996.12

植物の病原体認識とシグナル伝達 (植物のシグナルトランスダクション--分子機構と実験法) -- (細胞認識のシグナリング)

豊田和弘, 白石友紀, 山田哲治

現代化学増刊 ( 30 ) 185 - 194 1996.11

植物による病原菌の認識機構

白石友紀, 山田哲治, 一瀬勇規, 木場章範, 豊田和弘

日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan 60 71 - 71 1996.10

植物レクチンコンカナバリンAによるエリシター結合蛋白質の分離

豊田和弘, 杉本恵, 一瀬勇規, 山田哲治, 白石友紀

日本分子生物学会年会プログラム・講演要旨集 19 631 - 631 1996.8

生物発光Pseudomonas cichoriiのレタス葉における挙動

曳地康史, 那須佳子, 奥村亜子, 豊田和弘, 鈴木一実, 堀越守, 廣岡卓

日本分子生物学会年会プログラム・講演要旨集 19 423 - 423 1996.8

Involvement of Active Oxygen Species during the Plantpathogen Interaction : (II) Specific Inhibition of Superoxide Generation in Plant Cell Wall by Fungal Suppressor

KIBA A., MIYAKE C., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 62 ( 3 ) 292 - 292 1996.6

Recognition of Suppressor from Mycosphaerella pinodes by Pea Plants : Analysis of Supprescin A-binding Molecule (s) in Pea Cell Wall by RMD

SUGIURA T., AOYAGI M., KIBA A., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 62 ( 3 ) 292 - 292 1996.6

Characterization of Concanavalin A-binding Protein Complex from Pea Plasma Membranes : (III) An Actin-binding Protein in Con A-binding Protein Complex as Determined by Far Western Blotting with a Biotinylated Actin

TOYODA K., SUGIMOTO M., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 62 ( 3 ) 290 - 290 1996.6

SUPEROXIDE GENERATION IN CELL WALL DURING A PLANT-PATHOGEN INTERACTION

KIBA Akinori, MIYAKE Chizu, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

37 127 - 127 1996.3

PURIFICATION AND CHARACTERIZATION OF A BINDING PROTEIN FOR CONCANAVALIN A THAT ELICITS PHYTOALEXIN PRODUCTION IN PEA(PISUM SATIVUM L. )

TOYODA Kazuhiro, SUGIMOTO Megumi, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

37 15 - 15 1996.3

ASSOCIATION BETWEEN DEFENSE RESPONSES AND ION-FLUX(II)

AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

37 57 - 57 1996.3

Recognition of Pathogens and Signal Transduction in Higher Plants

Tokyo kagaku dojin 185 - 194 1996

植物防御応答にかかわるシグナル伝達機構-病原菌シグナルによる制御-(共著)

植物感染生理学の現状と将来展望日本植物病理学会 131 - 140 1996

植物の病原体認識とシグナル伝達(共著)

現代化学・増刊30植物のシグナルトランスダクション-分子機構と実験法-東京化学同人 185 - 194 1996

Speciesspecific suppression of superoxide-anion generation of surfaces of pea leaves by the suppressor from Mycosphaerella pinodes

KIBA A.

Ann. Phytopathol. Sco. Jpn. 62 ( 5 ) 508 - 512 1996

Signal Transduction Pathways of Higher Plants Are Regulated by Fungal Signals

Actual and Future Perspectives in Physiological Plant Pathology, The Phytopathological Society of Japan 131 - 140 1996

Regulation of Defense Responses in Pea by Fungal Singals, Elicitor and Suppressor from Mycosphaerella pinodes : 9 Involvement of diacylglycerol during Defense Responses

KAWAHARA T., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 613 - 613 1995.12

Involvement of Active Oxigen Species during the Plant-pathogen Interaction : Specific Suppression of Superoxide Generation by the Suppressor from Mycosphaerella pinodes

KIBA A., YAMAGUCHI Y., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 612 - 612 1995.12

Inhibition of a Phytoalexin Accumulation in Pea Eepicotyl Tissues by Cytochalasins

SUGIMOTO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 613 - 613 1995.12

The Role of Sugar Moieties of Elicitor and Suppressor in Plant-fungal Parasite Recognition : 3 Effect of Galactose on the Accumulation of Pisatin

YOSHIMOTO Y., TOYODA K., KATO T., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 614 - 614 1995.12

Characterization of Concanavalin A-binding protein Complex from Pea Plasma Membranes : II Phosphatidylinositol Kinase, Plasma Membrane ATPase, Small GTP-binding Proteins, Nucleoside Diphosphate Kinase G-actin and Certain Catalytic Subunits of Protein Kinase C are Associated with the Binding Protein of Concanavalin A That Elicits a Defense Response in Pea

TOYODA K., SUGIMOTO M., KAMEI A., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 614 - 614 1995.12

Effect of Suppressor from Mycosphaerella pinodes on the ATPase Activity in Microsomal Fraction from the Fungus

MISE D., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 615 - 615 1995.12

Relation between Defense Responses and Ion Flux : III. Rapid Change in Ion-flux on Cowpea Tissues Treated with the Suppressor from Mycosphaerella pinodes

AMANO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 612 - 612 1995.12

H^+-translocating Activity in Proteoliposomes Reconstituted with Pea Plasma Membrane ATPase and Its Inhibition by Fungal Suppressor from Mycosphaerella pinodes

AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Annals of the Phytopathological Society of Japan 61 ( 4 ) 369 - 375 1995.8

Language：English Publisher：The Phytopathological Society of Japan (PSJ)

Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on the H^+-translocating activity of ATPase in pea plasma membranes were examined. The plasma membrane ATPase was solubilized with Triton X-100 and partially purified by continuous glycerol density gradient centrifugation. The ATPase fraction was reconstituted into soybean phospholipid (asolectin) liposomes by a Triton-X 100 dilution method. Almost all of resultant proteoliposome vesicles had unimembranes in size from 50 to 200 nm. The ATP-driven H^+-pumping activity was measured by quinacrine fluorescence quenching in the presence of Mg-<2+>-ATP. The activity was sensitive to orthovanadate, dicyclohexylcar-bodiimide (DCCD), verapamil and neomycin but it was insensitive to azide and nitrate as well as ATP-hydrolyzing activity. Proton gradient was collapsed by NH_4Cl or a channel-forming ionophore, gramicidin D. The H^+-pumping activity in proteoliposomes was hardly affected by the elicitor. The finding suggests that a putative target molecule of or receptor for the elicitor might not associated with the solubilized ATPase or that the elicitor could not reach the target site. However, the suppressor markedly inhibited both activities in proteoliposomes, showing that the H^+-translocating ATPase might be inhibited directly in vitro by the suppressor as reported previously and that the fungal suppressor may disturb the regulation of pH in the host cells. Both activities were increased by the addition of phosphatidylinositolbisphosphate, even if in the presence of the suppressor. However, PIP_2 could not completely negate the effect of the suppressor. These results suggest that the H^+-pumping activity of plasma membrane ATPase is also regulated by polyphosphoinositide metabolism and that the action sites of the suppressor on the ATPase may be different from those of PIP_2.

Other Link： http://agriknowledge.affrc.go.jp/RN/2010521200

PLANT-LECTINS INDUCE THE PRODUCTION OF A PHYTOALEXIN IN PISUM-SATIVUM

K TOYODA, K MIKI, Y ICHINOSE, T YAMADA, T SHIRAISHI

PLANT AND CELL PHYSIOLOGY 36 ( 5 ) 799 - 807 1995.7

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

The effects of several plant lectins on the production of a pea phytoalexin, pisatin, were examined. Con A, PHA, PNA and PSA each induced the production of pisatin in pea epicotyl tissues, demonstrating that plant lectins can act as elicitors. The production of pisatin in response to PHA, PNA or PSA was not affected by the simultaneous presence of the respective hapten sugars, whereas haptens specific for Con A, such as alpha-D-mannose and methyl-alpha-D-mannoside, abolished the induction of pisatin by Con A. These results indicate that the elicitor effect of Con A is attributable to its ability to bind to specific carbohydrates in pea cells. Induction of the production of pisatin by Con A was markedly inhibited by the suppressor derived from a pea pathogen. Mycosphaerella pinodes, and by several inhibitors related to signal-transduction pathways. It is suggested, therefore, that the Con A-induced production of pisatin in pea tissues might be associated with activation of a signal-transduction pathway. An additive effect on the accumulation of pisatin was observed when Con A was present with a polysaccharide elicitor from M. pinodes, suggesting that exogenous Con A does not compete with the recognition site(s) for the fungal elicitor in pea cells. The present data also indicate that Con A may be useful for characterization of the signal-transduction system that leads to the synthesis of phytoalexin in pea epicotyl tissues.

SPECIFIC-INHIBITION OF CELL WALL-BOUND ATPASES BY FUNGAL SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

A KIBA, K TOYODA, Y ICHINOSE, T YAMADA, T SHIRAISHI

PLANT AND CELL PHYSIOLOGY 36 ( 5 ) 809 - 817 1995.7

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Activities of phosphatases were found in the fractions which were solubilized from cell walls of both pea and cowpea seedlings with 0.5 M NaCl. These phosphatases hydrolyzed triphosphonucleotides in the order: UTP = CTP > GTP > ATP; and UTP = GTP > CTP = ATP, respectively. The activities of a pyrophosphatase and a p-nitrophenylphosphatase were also detected in these fractions. The suppressor in the spore germination fluid of a pea pathogen, Mycosphaerella pinodes, inhibited all of these phosphatase activities in the fraction solubilized from pea cell walls, but it rather enhanced only the activity of the ATPase among those phosphatases from the cowpea cell wall. Hydrolysis of ATP by a cell wall fraction of pea was also markedly inhibited by the suppressor, while hydrolysis of ATP by similar fractions from cowpea, kidney bean and soybean were rather enhanced by the suppressor, as well as by the elicitor. Thus, the cell wall-bound ATPases responded to the suppressor species-specifically. These cell wall-bound ATPases seemed to be different from the plasma membrane ATPases in several respects. The results suggest that plants recognize the fungal signals not only on their plasma membranes but also on their cell walls and, moreover that putative receptors for the fungal signals might be located close to cell wall-bound ATPases or might even be these ATPases themselves.

Relationship between Defense Responses and Ion Flux : (II) Papid Na^+-Flux in Pea Tissues Treated with the Elicitor

AMANO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 247 - 247 1995.6

Specific Inhibition of Cell Wall-Bound ATPase by the Suppressor from Pea Pathogen mYCOSHAERELLA PINODES : (3) Effect of the Suppressor on ATPase Partially Purifed from Cell Walls

KIBA A., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 246 - 247 1995.6

The Role of Sugar Moieties of Elicitor and Suppressor in Plant-Fungal Parasite Recognition : (2) Effect of Monosaccharides on the Accumulation of Pisatin

YOSHIMOTO Y., TOYODA K., KATO T., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 247 - 247 1995.6

Characterization of Concanavalin A-Binding Protein Complex from Pea Plasma Membranes

TOYODA K., MIKI K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 247 - 247 1995.6

RELATIONSHIP BETWEEN DEFENSE RESPONSESAND ION FLUX

AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

36 S119 1995.3

Mechanism on Recognition of Elicitor : Lectins Stimulate Phytoalexin Production

MIKI K, TOYODA K, ICHINOSE Y, YAMADA T, SHIRAISHI T

Annals of the Phytopathological Society of Japan 60 ( 6 ) 767 - 767 1994.12

Effect of Orthovanadate in Pea Suspension-Cultured Cells

KAWAHARA T, TOYODA K, ISHINOSE Y, YAMADA T, SHIRAISHI T

Annals of the Phytopathological Society of Japan 60 ( 6 ) 766 - 766 1994.12

Relationship between Defense Responses and Ion Flux : (I). Effect of Several Ionophores and Ionchannel Blockers on the Accumulation of Pisatin

AMANO M, TOYODA K, YAMADA T, ICHINOSE Y, YAMADA T, SHIRAISHI T

Annals of the Phytopathological Society of Japan 60 ( 6 ) 766 - 767 1994.12

SUPPRESSORS - DETERMINANTS OF SPECIFICITY PRODUCED BY PLANT-PATHOGENS

T SHIRAISHI, T YAMADA, K SAITOH, T KATO, K TOYODA, H YOSHIOKA, HM KIM, Y ICHINOSE, M TAHARA, H OKU

PLANT AND CELL PHYSIOLOGY 35 ( 8 ) 1107 - 1119 1994.12

Language：English Publishing type：Book review, literature introduction, etc. Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Plant pathogens secrete suppressors that delay or prevent the host defense responses, with resultant conditioning of host cells such that they become susceptible even to avirulent or nonpathogenic microorganisms. Suppressors have been characterized as glycoproteins, glycopeptides, peptides and anionic and nonanionic glucans. A suppressor itself is non-toxic to plant cells and, thus, it can be distinguished from host-specific toxins produced by certain pathogens. Suppressors disturb fundamental functions of host plasma membranes. For example, the suppressor from a pea pathogen, Mycosphaerella pinodes, inhibits both the ATPase activity and polyphosphoinositide metabolism in pea plasma membranes, causing the temporary suppression of the signal-transduction pathway that leads to the expression of defense genes, which encode key enzymes in the biosynthetic pathway to phytoalexin. In this review, evidence for the role of suppressors in the determination of plant host-parasite specificity is summarized.