Faculty Profiles - TOYODA Kazuhiro

MISC - TOYODA Kazuhiro

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Recognition of Pathogens and Signal Transduction in Higher Plants

Tokyo kagaku dojin 185 - 194 1996

植物防御応答にかかわるシグナル伝達機構-病原菌シグナルによる制御-(共著)

植物感染生理学の現状と将来展望日本植物病理学会 131 - 140 1996

植物の病原体認識とシグナル伝達(共著)

現代化学・増刊30植物のシグナルトランスダクション-分子機構と実験法-東京化学同人 185 - 194 1996

Speciesspecific suppression of superoxide-anion generation of surfaces of pea leaves by the suppressor from Mycosphaerella pinodes

KIBA A.

Ann. Phytopathol. Sco. Jpn. 62 ( 5 ) 508 - 512 1996

Signal Transduction Pathways of Higher Plants Are Regulated by Fungal Signals

Actual and Future Perspectives in Physiological Plant Pathology, The Phytopathological Society of Japan 131 - 140 1996

Regulation of Defense Responses in Pea by Fungal Singals, Elicitor and Suppressor from Mycosphaerella pinodes : 9 Involvement of diacylglycerol during Defense Responses

KAWAHARA T., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 613 - 613 1995.12

Involvement of Active Oxigen Species during the Plant-pathogen Interaction : Specific Suppression of Superoxide Generation by the Suppressor from Mycosphaerella pinodes

KIBA A., YAMAGUCHI Y., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 612 - 612 1995.12

Inhibition of a Phytoalexin Accumulation in Pea Eepicotyl Tissues by Cytochalasins

SUGIMOTO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 613 - 613 1995.12

The Role of Sugar Moieties of Elicitor and Suppressor in Plant-fungal Parasite Recognition : 3 Effect of Galactose on the Accumulation of Pisatin

YOSHIMOTO Y., TOYODA K., KATO T., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 614 - 614 1995.12

Characterization of Concanavalin A-binding protein Complex from Pea Plasma Membranes : II Phosphatidylinositol Kinase, Plasma Membrane ATPase, Small GTP-binding Proteins, Nucleoside Diphosphate Kinase G-actin and Certain Catalytic Subunits of Protein Kinase C are Associated with the Binding Protein of Concanavalin A That Elicits a Defense Response in Pea

TOYODA K., SUGIMOTO M., KAMEI A., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 614 - 614 1995.12

Effect of Suppressor from Mycosphaerella pinodes on the ATPase Activity in Microsomal Fraction from the Fungus

MISE D., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 615 - 615 1995.12

Relation between Defense Responses and Ion Flux : III. Rapid Change in Ion-flux on Cowpea Tissues Treated with the Suppressor from Mycosphaerella pinodes

AMANO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 6 ) 612 - 612 1995.12

H^+-translocating Activity in Proteoliposomes Reconstituted with Pea Plasma Membrane ATPase and Its Inhibition by Fungal Suppressor from Mycosphaerella pinodes

AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

Annals of the Phytopathological Society of Japan 61 ( 4 ) 369 - 375 1995.8

Language：English Publisher：The Phytopathological Society of Japan (PSJ)

Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on the H^+-translocating activity of ATPase in pea plasma membranes were examined. The plasma membrane ATPase was solubilized with Triton X-100 and partially purified by continuous glycerol density gradient centrifugation. The ATPase fraction was reconstituted into soybean phospholipid (asolectin) liposomes by a Triton-X 100 dilution method. Almost all of resultant proteoliposome vesicles had unimembranes in size from 50 to 200 nm. The ATP-driven H^+-pumping activity was measured by quinacrine fluorescence quenching in the presence of Mg-<2+>-ATP. The activity was sensitive to orthovanadate, dicyclohexylcar-bodiimide (DCCD), verapamil and neomycin but it was insensitive to azide and nitrate as well as ATP-hydrolyzing activity. Proton gradient was collapsed by NH_4Cl or a channel-forming ionophore, gramicidin D. The H^+-pumping activity in proteoliposomes was hardly affected by the elicitor. The finding suggests that a putative target molecule of or receptor for the elicitor might not associated with the solubilized ATPase or that the elicitor could not reach the target site. However, the suppressor markedly inhibited both activities in proteoliposomes, showing that the H^+-translocating ATPase might be inhibited directly in vitro by the suppressor as reported previously and that the fungal suppressor may disturb the regulation of pH in the host cells. Both activities were increased by the addition of phosphatidylinositolbisphosphate, even if in the presence of the suppressor. However, PIP_2 could not completely negate the effect of the suppressor. These results suggest that the H^+-pumping activity of plasma membrane ATPase is also regulated by polyphosphoinositide metabolism and that the action sites of the suppressor on the ATPase may be different from those of PIP_2.

CiNii Article

CiNii Books

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Other Link： http://agriknowledge.affrc.go.jp/RN/2010521200

PLANT-LECTINS INDUCE THE PRODUCTION OF A PHYTOALEXIN IN PISUM-SATIVUM

K TOYODA, K MIKI, Y ICHINOSE, T YAMADA, T SHIRAISHI

PLANT AND CELL PHYSIOLOGY 36 ( 5 ) 799 - 807 1995.7

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

The effects of several plant lectins on the production of a pea phytoalexin, pisatin, were examined. Con A, PHA, PNA and PSA each induced the production of pisatin in pea epicotyl tissues, demonstrating that plant lectins can act as elicitors. The production of pisatin in response to PHA, PNA or PSA was not affected by the simultaneous presence of the respective hapten sugars, whereas haptens specific for Con A, such as alpha-D-mannose and methyl-alpha-D-mannoside, abolished the induction of pisatin by Con A. These results indicate that the elicitor effect of Con A is attributable to its ability to bind to specific carbohydrates in pea cells. Induction of the production of pisatin by Con A was markedly inhibited by the suppressor derived from a pea pathogen. Mycosphaerella pinodes, and by several inhibitors related to signal-transduction pathways. It is suggested, therefore, that the Con A-induced production of pisatin in pea tissues might be associated with activation of a signal-transduction pathway. An additive effect on the accumulation of pisatin was observed when Con A was present with a polysaccharide elicitor from M. pinodes, suggesting that exogenous Con A does not compete with the recognition site(s) for the fungal elicitor in pea cells. The present data also indicate that Con A may be useful for characterization of the signal-transduction system that leads to the synthesis of phytoalexin in pea epicotyl tissues.

Web of Science

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SPECIFIC-INHIBITION OF CELL WALL-BOUND ATPASES BY FUNGAL SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

A KIBA, K TOYODA, Y ICHINOSE, T YAMADA, T SHIRAISHI

PLANT AND CELL PHYSIOLOGY 36 ( 5 ) 809 - 817 1995.7

Language：English Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Activities of phosphatases were found in the fractions which were solubilized from cell walls of both pea and cowpea seedlings with 0.5 M NaCl. These phosphatases hydrolyzed triphosphonucleotides in the order: UTP = CTP > GTP > ATP; and UTP = GTP > CTP = ATP, respectively. The activities of a pyrophosphatase and a p-nitrophenylphosphatase were also detected in these fractions. The suppressor in the spore germination fluid of a pea pathogen, Mycosphaerella pinodes, inhibited all of these phosphatase activities in the fraction solubilized from pea cell walls, but it rather enhanced only the activity of the ATPase among those phosphatases from the cowpea cell wall. Hydrolysis of ATP by a cell wall fraction of pea was also markedly inhibited by the suppressor, while hydrolysis of ATP by similar fractions from cowpea, kidney bean and soybean were rather enhanced by the suppressor, as well as by the elicitor. Thus, the cell wall-bound ATPases responded to the suppressor species-specifically. These cell wall-bound ATPases seemed to be different from the plasma membrane ATPases in several respects. The results suggest that plants recognize the fungal signals not only on their plasma membranes but also on their cell walls and, moreover that putative receptors for the fungal signals might be located close to cell wall-bound ATPases or might even be these ATPases themselves.

Web of Science

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Relationship between Defense Responses and Ion Flux : (II) Papid Na^+-Flux in Pea Tissues Treated with the Elicitor

AMANO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 247 - 247 1995.6

Specific Inhibition of Cell Wall-Bound ATPase by the Suppressor from Pea Pathogen mYCOSHAERELLA PINODES : (3) Effect of the Suppressor on ATPase Partially Purifed from Cell Walls

KIBA A., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 246 - 247 1995.6

The Role of Sugar Moieties of Elicitor and Suppressor in Plant-Fungal Parasite Recognition : (2) Effect of Monosaccharides on the Accumulation of Pisatin

YOSHIMOTO Y., TOYODA K., KATO T., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 247 - 247 1995.6

Characterization of Concanavalin A-Binding Protein Complex from Pea Plasma Membranes

TOYODA K., MIKI K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

Annals of the Phytopathological Society of Japan 61 ( 3 ) 247 - 247 1995.6

RELATIONSHIP BETWEEN DEFENSE RESPONSESAND ION FLUX

AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

36 S119 1995.3

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