Faculty Profiles - HANAMOTO Katsumi

The Effects of Low-Dose-Rate γ-irradiation on Forced Swim Test-Induced Immobility and Oxidative Stress in Mice.

Tetsuya Nakada, Takahiro Kataoka, Takaharu Nomura, Hina Shuto, Junki Yano, Shota Naoe, Katsumi Hanamoto, Kiyonori Yamaoka

Acta medica Okayama 75 ( 2 ) 169 - 175 2021.4

Language：English Publishing type：Research paper (scientific journal)

The forced swim test (FST) induces immobility in mice. Low-dose (high-dose-rate) X-irradiation inhibits FSTinduced immobility in mice due to its antioxidative function. We evaluated the effects of low-dose γ-irradiation at a low-dose-rate on the FST-induced depletion of antioxidants in mouse organs. Mice received whole-body low-dose-rate (0.6 or 3.0 mGy/h) of low-dose γ-irradiation for 1 week, followed by daily FSTs (5 days). The immobility rate on day 2 compared to day 1 was significantly lower in the 3.0 mGy/h irradiated mice than in sham irradiated mice. The FST significantly decreased the catalase (CAT) activity and total glutathione (t-GSH) content in the brain and kidney, respectively. The superoxide dismutase (SOD) activity and t-GSH content in the liver of the 3.0 mGy/h irradiated mice were significantly lower than those of the non-FST-treated mice. The CAT activity in the lungs of mice exposed to 3.0 mGy/h γ-irradiation was higher than that of non-FST treated mice and mice treated with FST. However, no significant differences were observed in the levels of these antioxidant markers between the sham and irradiated groups except for the CAT activity in lungs. These findings suggest that the effects of low-dose-rate and low-dose γ-irradiation on FST are highly organ-dependent.

DOI： 10.18926/AMO/61896

Evaluation of the redox state in mouse organs following radon inhalation

Takahiro Kataoka, Norie Kanzaki, Akihiro Sakoda, Hina Shuto, Junki Yano, Shota Naoe, Hiroshi Tanaka, Katsumi Hanamoto, Hiroaki Terato, Fumihiro Mitsunobu, Kiyonori Yamaoka

Journal of radiation research 62 ( 2 ) 206 - 216 2021.3

Publishing type：Research paper (scientific journal)

Radon inhalation activates antioxidative functions in mouse organs, thereby contributing to inhibition of oxidative stress-induced damage. However, the specific redox state of each organ after radon inhalation has not been reported. Therefore, in this study, we evaluated the redox state of various organs in mice following radon inhalation at concentrations of 2 or 20 kBq/m3 for 1, 3 or 10 days. Scatter plots were used to evaluate the relationship between antioxidative function and oxidative stress by principal component analysis (PCA) of data from control mice subjected to sham inhalation. The results of principal component (PC) 1 showed that the liver and kidney had high antioxidant capacity; the results of PC2 showed that the brain, pancreas and stomach had low antioxidant capacities and low lipid peroxide (LPO) content, whereas the lungs, heart, small intestine and large intestine had high LPO content but low antioxidant capacities. Furthermore, using the PCA of each obtained cluster, we observed altered correlation coefficients related to glutathione, hydrogen peroxide and LPO for all groups following radon inhalation. Correlation coefficients related to superoxide dismutase in organs with a low antioxidant capacity were also changed. These findings suggested that radon inhalation could alter the redox state in organs; however, its characteristics were dependent on the total antioxidant capacity of the organs as well as the radon concentration and inhalation time. The insights obtained from this study could be useful for developing therapeutic strategies targeting individual organs.

DOI： 10.1093/jrr/rraa129

Scopus

ラドン吸入によるマウス諸臓器中のDNA酸化損傷抑制の濃度依存に関する検討．

増川祐伎, 光延文裕, 寺東宏明, 山岡聖典, 片岡隆浩, 首藤妃奈, 直江翔太, 矢野凖喜, 神崎訓枝, 迫田晃弘, 田中裕史, 花元克巳

アイソトープ・放射線研究発表会 1 86 - 86 2021

Language：Japanese Publisher：公益社団法人日本アイソトープ協会

<p>ラドン吸入によるマウス諸臓器中のDNA酸化損傷の抑制について検討した結果，脳や腎臓において抑制することが示唆できた。これに関して，ラドン吸入により抗酸化酵素であるMn-SODの活性が増加しDNAの酸化損傷を抑制する機序や，ラドン濃度依存性のあることも示唆できた。</p>

DOI： 10.50955/happyokai.1.0_86

CiNii Article

X-Irradiation at 0.5 Gy after the forced swim test reduces forced swimming-induced immobility in mice

Takahiro Kataoka, Hina Shuto, Junki Yano, Shota Naoe, Tsuyoshi Ishida, Tetsuya Nakada, Keiko Yamato, Katsumi Hanamoto, Takaharu Nomura, Kiyonori Yamaoka

Journal of Radiation Research 61 ( 4 ) 517 - 523 2020.7

Publishing type：Research paper (scientific journal)

The forcedswimtest (FST)is a screeningmodel forantidepressantactivity; it causes immobilityand inducesoxidative stress. We previously reported that radon inhalation has antidepressant-like effects in mice potentially through the activation of antioxidative functions upon radon inhalation. This study aimed to investigate the effect of prior and post low-dose X-irradiation (0.1, 0.5, 1.0 and 2.0 Gy) on FST-induced immobility and oxidative stress in the mouse brain, and the differences, if any, between the two.Mice received X-irradiation before or after the FST repeatedly for 5 days. In the post-FST-irradiated group, an additional FST was conducted 4 h after the last irradiation.Consequently, animals receiving priorX-irradiation (0.1Gy) had better mobility outcomes than sham-irradiatedmice; however, their levels of lipid peroxide (LPO), an oxidative stressmarker, remained unchanged.However, animals that received post- FST X-irradiation (0.5 Gy) had better mobility outcomes and their LPO levels were significantly lower than those of the sham-irradiated mice. The present results indicate that 0.5 Gy X-irradiation after FST inhibits FST-induced immobility and oxidative stress in mice.

DOI： 10.1093/jrr/rraa022

Scopus

Basic study on enhancement of antioxidant function by low-dose irradiation in mouse brain and its combined effect with ascorbic acid Reviewed

Takahiro KATAOKA, Yuto YUNOKI, Norie KANZAKI, Kaori SASAOKA, Yusuke KOBASHI, Tsuyoshi ISHIDA, Katsumi HANAMOTO, Kiyonori YAMAOKA

RADIOISOTOPES 69 ( 2 ) 45 - 53 2020.2

X線照射によるマウス脳の酸化ストレス状態の変化特性に関する検討

片岡隆浩, 神崎訓枝, 柚木勇人, 小橋佑介, 石田毅, 花元克巳, 山岡聖典

日本放射線影響学会大会講演要旨集 61回 78 - 78 2018.11

Pressure dependence of X-rays produced by an LiTaO3 single crystal over a wide range of pressure

Katsumi Hanamoto, Takahiro Kataoka, Kiyonori Yamaoka

Applied Radiation and Isotopes 135 40 - 42 2018.5

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier Ltd

The energy spectra of X-rays produced by an LiTaO3 single crystal have been measured at the pressures of 8 × 10−4−4 Pa. The energy spectra showed that the endpoint energy steadily increased with decrease of pressure at 2–4 Pa and gradually decreased with decrease of pressure at 0.1–2 Pa, and became almost constant below 0.1 Pa. The amount of X-rays steadily increased with decrease of pressure at 2–4 Pa and almost saturated below 0.5 Pa. The change of X-rays produced by the LiTaO3 single crystal was compared with the previous works and discussed in relation to the distance between the crystal surface and the target.

DOI： 10.1016/j.apradiso.2018.01.019

Scopus

Pressure dependence of X-rays produced by an LiTaO3 single crystal at the pressures of 1-20 Pa

Katsumi Hanamoto, Takahiro Kataoka, Kiyonori Yamaoka

APPLIED RADIATION AND ISOTOPES 116 134 - 137 2016.10

Language：English Publishing type：Research paper (scientific journal) Publisher：PERGAMON-ELSEVIER SCIENCE LTD

The energy spectra of X-rays produced by an LiTaO3 single crystal have been measured at pressures of 1-20 Pa. The energy spectra showed that the endpoint energy and the amount of X-rays gradually increased with the decrease of pressure at 5-8 Pa and abruptly increased around 4 Pa and below. A maximum endpoint energy of 70 keV was obtained using the LiTaO3 single crystal with 5 mm thickness at a pressure of 3 Pa. The change of X-rays produced by the LiTaO(3)single crystal was discussed in relation to the breakdown potential at the pressures. (C) 2016 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.apradiso.2016.08.003

Activation of Antioxidative Functions by Radon Inhalation Enhances the Mitigation Effects of Pregabalin on Chronic Constriction Injury-Induced Neuropathic Pain in Mice

Takahiro Kataoka, Shunsuke Horie, Reo Etani, Norie Kanzaki, Kaori Sasaoka, Yusuke Kobashi, Katsumi Hanamoto, Kiyonori Yamaoka

OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 2016 2016

Language：English Publishing type：Research paper (scientific journal) Publisher：HINDAWI LTD

Radon inhalation brings pain relief for chronic constriction injury- (CCI-) induced neuropathic pain in mice due to the activation of antioxidative functions, which is different from the mechanism of the pregabalin effect. In this study, we assessed whether a combination of radon inhalation and pregabalin administration is more effective against neuropathic pain than radon or pregabalin only. Mice were treated with inhaled radon at a concentration of 1,000 Bq/m(3) for 24 hours and pregabalin administration after CCI surgery. In mice treated with pregabalin at a dose of 3 mg/kg weight, the 50% paw withdrawal threshold of mice treated with pregabalin or radon and pregabalin was significantly increased, suggesting pain relief. The therapeutic effects of radon inhalation or the combined effects of radon and pregabalin (3 mg/kg weight) were almost equivalent to treatment with pregabalin at a dose of 1.4 mg/kg weight or 4.1 mg/kg weight, respectively. Radon inhalation and the combination of radon and pregabalin increased antioxidant associated substances in the paw. The antioxidant substances increased much more in radon inhalation than in pregabalin administration. These findings suggested that the activation of antioxidative functions by radon inhalation enhances the pain relief of pregabalin and that this combined effect is probably an additive effect.

DOI： 10.1155/2016/9853692

Evaluating the protective effects of radon inhalation or ascorbic acid treatment after transient global cerebral ischemic injury in gerbils

Takahiro Kataoka, Reo Etani, Norie Kanzaki, Kaori Sasaoka, Yusuke Kobashi, Katsumi Hanamoto, Takehito Taguchi, Kiyonori Yamaoka

JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY 53 ( 11 ) 1681 - 1685 2016

Language：English Publishing type：Research paper (scientific journal) Publisher：TAYLOR & FRANCIS LTD

In this study, we compared the protective effects of radon inhalation and ascorbic acid administration on transient global cerebral ischemic injury in gerbils. Gerbils were treated with radon inhalation (2000 Bq/m(3), 24 hours) or ascorbic acid (100, 300, or 500mg/kg body weight). Then, transient global cerebral ischemia was induced by bilateral occlusion of the common carotid artery. Results showed that the number of damaged neurons was significantly increased in gerbils that underwent ischemia compared with that in control gerbils. However, the number of damaged neurons in gerbils treated with radon or 500mg/kg of ascorbic acid before ischemia was significantly lower than gerbils who were subjected to ischemia without any pretreatment, and the protective effects of radon inhalation were similar to the effects of administering 500mg/kg ascorbic acid. The levels of superoxide dismutase (SOD) and total glutathione (t-GSH) in brain tissue were increased to a similar extent by pretreatment with radon inhalation or 500mg/kg of ascorbic acid. These findings suggested that radon inhalation has a protective antioxidative effect against transient global cerebral ischemic injury similar to 500mg/kg ascorbic acid treatment.

DOI： 10.1080/00223131.2016.1198731

Effects of cream containing ultralow volume radionuclides on carrageenan-induced inflammatory paw edema in mice Reviewed

Takahiro Kataoka, Yuji Takata, Reo Etani, Yuichi Nishiyama, Atsushi Kawabe, Katsumi Hanamoto, Takehito Taguchi, Kiyonori Yamaoka

Biochemistry & Physiology 3 ( 2 ) 1 - 5 2014

Radioactivity of Pb-210 in Japanese cigarettes and radiation dose from smoking inhalation

A. Sakoda, K. Fukao, A. Kawabe, T. Kataoka, K. Hanamoto, K. Yamaoka

RADIATION PROTECTION DOSIMETRY 150 ( 1 ) 109 - 113 2012.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

It is well known that cigarette tobaccos contain naturally occurring radioactive nuclides such as Pb-210 and Po-210. In many countries, the radioactivity of tobaccos has been measured to estimate the effective dose from smoking inhalation. The present study covered 24 cigarette brands including the top 20 of sales in Japan between April 2008 and March 2009. The activity concentrations of Pb-210 were measured by gamma-ray spectrometry, and then those of its progeny (Po-210) were evaluated assuming the radioactive equilibrium between the two nuclides. Their concentrations were in the range of 214 mBq cigarette(1) with an arithmetic mean of 83 mBq cigarette(1). The annual committed effective doses were also calculated, based on the scenario that a smoker consumes 20 cigarettes a day. The average doses from Pb-210 and Po-210 inhalations were 229 and 6827 Sv y(1), respectively.

DOI： 10.1093/rpd/ncr364

Pressure dependence of X-rays produced by an LiTaO3 single crystal at low pressures

Katsumi Hanamoto, Atsushi Kawabe, Akihiro Sakoda, Takahiro Kataoka, Mari Okada, Kiyonori Yamaoka

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT 669 66 - 69 2012.3

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

The energy spectra of X-rays produced by an LiTaO3 single crystal have been measured at pressures of 5-50 Pa. The energy spectra showed that the amount of X-rays increased exponentially and the endpoint energy of the spectra increased linearly with the decrease of pressure at pressures of 10-25 Pa. A maximum endpoint energy of about 22 key was obtained using an LiTaO3 single crystal with 0.5 mm thickness at the pressure of 10 Pa. The maximum energy produced by the present experimental setup was estimated assuming that the LiTaO3 single crystal forms a parallel-plate capacitor. The estimated energy reasonably agreed with the energy obtained. The pressure dependence of the endpoint energy was discussed in relation to the breakdown potential at low pressures. (C) 2011 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.nima.2011.12.028

ラドン吸入がペットの健康改善に及ぼす効果に関する基礎的検討

片岡隆浩, 徳永力三, 迫田晃弘, 川辺睦, 花元克巳, 山岡聖典

Radioisotopes 61 ( 1 ) 3 - 10 2012.1

Comparative Study on the Inhibitory Effects of alpha-Tocopherol and Radon on Carbon Tetrachloride-Induced Renal Damage

Takahiro Kataoka, Keiko Yamato, Yuichi Nishiyama, Yuji Morii, Reo Etani, Yuji Takata, Katsumi Hanamoto, Atsuishi Kawabe, Akihiro Sakoda, Yuu Ishimori, Takehito Taguchi, Kiyonori Yamaoka

RENAL FAILURE 34 ( 9 ) 1181 - 1187 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：TAYLOR & FRANCIS LTD

Since the 2011 nuclear accident in Fukushima, the effects of low-dose irradiation, especially internal exposure, are at the forefront of everyone's attention. However, low-dose radiation induced various stimulating effects such as activation of antioxidative and immune functions. In this study, we attempted to evaluate the quantitative effects of the activation of antioxidative activities in kidney induced by radon inhalation on carbon tetrachloride (CCl4)-induced renal damage. Mice were subjected to intraperitoneal (i.p.) injection of CCl4 after inhaling approximately 1000 or 2000 Bq/m(3) radon for 24 h, or immediately after i.p. injection of alpha-tocopherol (100, 300, or 500 mg/kg bodyweight). In case of renal function, radon inhalation at a concentration of 2000 Bq/m(3) has the inhibitory effects similar to alpha-tocopherol treatment at a dose of 300-500 mg/kg bodyweight. The activities of superoxide dismutase and catalase in kidneys were significantly higher in mice exposed to radon as compared to mice treated with CCl4 alone. These findings suggest that radon inhalation has an antioxidative effect against CCl4-induced renal damage similar to the antioxidative effects of alpha-tocopherol due to induction of antioxidative functions.

DOI： 10.3109/0886022X.2012.717496