Faculty Profiles - TAKASHIBA Shogo

Papers - TAKASHIBA Shogo

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細菌感染度を評価しながら包括的歯周治療を行った広汎型侵襲性歯周炎患者の一症例 Reviewed

冨川和哉, 河野隆幸, 山本直史, 岩本義博, 下江正幸, 山口知子, 本郷昌一, 宮本学, 前田博史, 高柴正悟

日本歯周病学会会誌 55 ( 4 ) 340 - 348 2013.12

Authorship：Last author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(NPO)日本歯周病学会

歯周病に対する感受性が高い侵襲性歯周炎患者の治療においては、徹底した感染のコントロールによる疾患活動性の抑制が必要である。とりわけ、歯列不正を伴う歯周炎患者に矯正治療を行うことは、長期に渡る感染のコントロールを行う上で非常に有効である。しかし、歯周炎患者に矯正治療を行う場合に、感染がコントロールされている歯周状態かどうかを決定する基準は未だ明確でない。今回報告するのは、Agregatibacter actinomycetemcomitans(Aa)の感染が主な病態形成因子と考えられる、初診時22歳の広汎型侵襲性歯周炎患者に対して、矯正治療を含めた包括的歯周治療を行った症例である。初診以降、臨床計測値の変化に加えて、細菌DNA検査と歯周病原細菌に対する血清IgG抗体価検査を用いて、歯周病原細菌の感染度を評価した。その結果、歯周外科治療を含む感染源除去によって、細菌DNA検査でAaが検出されず、Aaに対する血清IgG抗体価が健常者レベルに推移したことを矯正治療移行前に確認した。すなわち、歯周病原細菌感染度が低下したと判断した後に、矯正治療を行うことで、現在まで良好なSPTを維持している。本症例においては、Aaの感染度の低下を細菌DNA検査と血清IgG抗体価を用いて評価することによって、客観的な根拠をもって矯正治療に移行できたと考える。(著者抄録)

DOI： 10.2329/perio.55.340

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高齢者病棟および高齢者施設における歯科医療職の人材配置 Reviewed

目黒道生, 冨山祐佳, 小出康史, 小林芳友, 小林直樹, 藤原ゆみ, 岩田宏隆, 苅田典子, 久保克行, 佐藤公麿, 山部こころ, 山本大介, 澤田弘一, 高柴正悟, 松尾浩一郎

老年歯科医学 28 ( 2 ) 79 - 87 2013.9

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(一社)日本老年歯科医学会

近年、病院や高齢者施設における医療職種の適切な人材配置が、医療の質や安全性の向上につながることが明らかになってきた。一方、歯科医療職の人材配置による入院患者の健康状態への影響については十分に検証されていない。そこで、今回われわれは、基礎的調査として、高齢者対象の病棟および施設こおける職種ごとの人材配置の現状を把握し、歯科医療職と他職種との相違を比較した。岡山県および広島県内の1施設および4病院に勤務する医療職274名を対象に、就業時間と業務内容に関する無記名自記式アンケート調査を実施した。入院患者に関わる業務は、直接業務、書類業務、環境業務の3業務に分類した。また、各々の業務に関わる時間を算出し、入院患者に関わる合計時間から入院病床数100床あたりの医療職の人数を算出し、スタッフ/病床比と定義した。平均スタッフ/病床比は、介護職と看護師において12.3および9.3と高値を示した一方で、歯科医師と歯科衛生士では、0.3および0.6と低かった。さらに、歯科衛生士では入院患者に接する直接業務時間も有意に短かった。本結果より、歯科医療職は、他職種と比して病棟への人材配置が少ないことが明らかになり、介入が不足している可能性が示唆された。「口腔環境と全身疾患との関連性について多くの報告がなされるなか、病院、施設において、歯科医療職の入院患者への業務時間を増やす取り組みが必要であると考える。(著者抄録)

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Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT Reviewed

Hirai, K., Maeda, H., Omori, K., Yamamoto, T., Kokeguchi, S., Takashiba, S.

Pathogens and Disease 68 ( 1 ) 12 - 19 2013.6

Medical microbiological approach to Archaea in oralinfectious diseases

Maeda Hiroshi, Hirai Kimito, Mineshiba Junji, Yamamoto Tadashi, Kokeguchi Susumu, Takashiba Shogo

49 ( 1 ) 72 - 78 2013.5

Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array Reviewed

Sato, T., Soga, Y., Yamaguchi, T., Meguro, M., Maeda, H., Tada, J., Otani, T., Seno, M., Takashiba, S.

Asian Pacific Journal of Allergy and Immunology 31 ( 4 ) 2013.4

露出根面と義歯表面をターゲットとした抗菌対策抗菌物質のドラッグデリバリーシステムとバイオフィルム付着防止材の開発 Invited Reviewed

高柴正悟, 寺下正道, 松尾敬志, 寺中敏夫, 田中隆博, 畑中加珠, 難波尚子

日本歯科医学会誌 32 54 - 58 2013.3

Authorship：Lead author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本歯科医学会

高齢者の歯科医療現場においては、歯周病の進行に伴い露出した根面や義歯表面に多量のプラークが付着していることが多く見受けられる。このことが原因となって、口腔内のトラブルを来していることがある。そこで、露出根面と義歯表面をターゲットとした抗菌性物質を開発し、高齢者医療での口腔細菌の感染対策を主体とした保存的治療を確立しようとした。まず、歯質接着性を有するリン酸化プルランを抗菌剤である塩化セチルピリジニウム(CPC)と混合することによって、歯面への細菌の付着を持続的に抑制することが明らかになった。実際の口腔内を想定した試験や動物における安全性試験などから実用性を確認し、大学発のベンチャー設立につながった。また、CPC配合の薬剤をタフトブラシと一体化した容器に封入した製剤を開発し、その臨床的および細菌学的効果をサンスター株式会社との産学連携の共同研究にて明らかにした。その成果から、第3類医薬品「G・U・mメディカルマージナルポイントケアA」が上市された。一方、義歯床用レジンにシリカをコーティングすることによって、細菌の付着を抑制することを試みた。低温でのシリカ添加の方法を樹立し、床用レジン上での安定性を確保することができたので、臨床応用に向けて取り組んでいるところである。以上から、高齢者医療における歯科保存治療技術・素材として、歯科診療への応用を臨学産連携で図ることができるようになってきた。今後は、学会主導でこれらの製品や技術を用いて、細菌感染対策の面から高齢者医療における歯科保存治療を行う指針を策定する段階が近づいていると思われる。(著者抄録)

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基礎研究から歯科臨床へ歯周病原細菌に対する血漿IgG抗体価検査の可能性 Invited Reviewed

大森一弘, 工藤値英子, 成石浩司, 前田博史, 高柴正悟

日本歯周病学会会誌 55 ( 1 ) 9 - 14 2013.3

Antibiotic sensitivity of bacteria on the oral mucosa after hematopoietic cell transplantation Reviewed

Soga Yoshihiko, Maeda Yoshinobu, Tanimoto Mitsune, Ebinuma Takayuki, Maeda Hiroshi, Takashiba Shogo

Supportive Care in Cancer 21 ( 2 ) 367 - 368 2013.2

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer-Verlag

DOI： 10.1007/s00520-012-1602-9

Other Link： http://link.springer.com/article/10.1007/s00520-012-1602-9/fulltext.html

Smad2 decelerates re-epithelialization during gingival wound healing Reviewed

Tomikawa, K., Yamamoto, T., Shiomi, N., Shimoe, M., Hongo, S., Yamashiro, K., Yamaguchi, T., Maeda, H., Takashiba, S.

Journal of Dental Research 91 ( 8 ) 764 - 770 2012.8

Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：SAGE Publications

During periodontal regeneration, inhibition of gingival downgrowth is necessary to promote migration of mesenchymal cells into the defects. Transforming growth factor (TGF)-β is a pleiotropic cytokine that has numerous cell functions, including regulation of epithelial growth. Recent studies have shown that Smad2, a downstream transcription factor of TGF-β, plays crucial roles in wound healing in the epithelia. Therefore, we investigated the effects of Smad2 overexpression on re-epithelialization of gingival wounds. Transgenic mice overexpressing smad2 driven by the keratin 14 promoter ( k14-smad2) were confirmed to have significant Smad2 phosphorylation in gingival basal epithelia. Punch wounds were made in the palatal gingiva, and wound healing was assessed histologically for 7 days. Re-epithelialization was significantly retarded on day 2, while collagen deposition was enhanced on day 7 in k14-smad2 compared with wild-type mice. Moreover, expression of keratin 16 (K16), an indicator of keratinocyte migration, was significantly inhibited in wound-edge keratinocytes in k14-smad2. The inhibition of K16 coincided with the induction of Smad2 in the corresponding epithelia, while BrdU incorporation was unaffected. These results indicated that Smad2 has inhibitory effects in regulating keratinocyte migration during gingival wound healing. TGF-β/Smad2 signaling mediating alteration of K16 expression must be tightly regulated during periodontal regeneration.

DOI： 10.1177/0022034512451449

Other Link： http://journals.sagepub.com/doi/full-xml/10.1177/0022034512451449

Relationship between periodontitis-related antibody and frequent exacerbations in chronic obstructive pulmonary disease Reviewed

Takahashi, T., Muro, S., Tanabe, N., Terada, K., Kiyokawa, H., Sato, S., Hoshino, Y., Ogawa, E., Uno, K., Naruishi, K., Takashiba, S., Mishima, M.

PLoS ONE 7 ( 7 ) e40570 - e40570 2012.7

Histological and immunohistochemical features of gingival enlargement in a patient with AML Reviewed

Sonoi, N., Soga, Y., Maeda, H., Ichimura, K., Yoshino, T., Aoyama, K., Fujii, N., Maeda, Y., Tanimoto, M., Logan, R., Raber-Durlacher, J., Takashiba, S.

Odontology 100 ( 2 ) 254 - 257 2012.7

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

DOI： 10.1007/s10266-011-0051-0

Other Link： http://link.springer.com/article/10.1007/s10266-011-0051-0/fulltext.html

15-year Clinical Course of a Patient with Generalized Aggressive Periodontitis Followed with Regular examination of the serum titers of IgG antibodies against periodontal bacteria Reviewed

TOMIKAWA Kazuya, IWAMOTO Yoshihiro, OHE Hyogo, ARAI Hideo, YAMAMOTO Tadashi, MAEDA Hiroshi, TAKASHIBA Shogo

Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 54 ( 2 ) 193 - 202 2012.6

Authorship：Last author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：特定非営利活動法人日本歯周病学会

For the treatment of patients with aggressive periodontitis, a thorough control of the infection is required to reduce the disease activity. Detection of periodontal pathogens by a polymerase chain reaction-based method or measurement of the serum IgG antibody titers by enzyme-linked immunosorbent assay are now widely used for microbiological diagnosis and assessment of the disease activity. We report the case of a 25-year-old woman with impaired neutrophil phagocytosis ability who suffered from generalized aggressive periodontitis . After the initial periodontal treatment and periodontal regenerative surgery, she received the final prosthodontics, and supportive periodontal treatment (SPT) was initiated. For over a decade, from the beginning of the treatments, the clinical parameters and serum titers of IgG periodontal pathogens have been monitored, and the relationships between the twoanalyzed. During the SPT, recurrence and progression of periodontitis was seen around site 26, despite maintenance of a stable periodontal condition at other sites. The serum IgG antibody titers against periodontal pathogens were elevated in parallel with the progression of periodontitis at site 26. In the present case of generalized aggressive periodontitis, the serum IgG antibody titers reflected the disease activity of periodontitis even around a single tooth, suggesting the usefulness of the examination for assessment of the disease activity. Nihon Shishubyo Gakkai Kaishi(J Jpn Soc Periodontol)54(2):193-202, 2012

DOI： 10.2329/perio.54.193

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歯周炎罹患歯に対するFGF-2投与の長期的効果および安全性の検討 Reviewed

北村正博, 古市保志, 藤井健男, 川浪雅光, 國松和司, 島内英俊, 山田了, 小方頼昌, 和泉雄一, 伊藤公一, 中川種昭, 新井高, 山崎和久, 吉江弘正, 野口俊英, 渋谷俊昭, 高柴正悟, 栗原英見, 永田俊彦, 横田誠, 前田勝正, 廣藤卓雄, 坂上竜資, 原宜興, 野口和行, 小笠原健文, 村上信也

日本歯周病学会会誌 54 ( 1 ) 38 - 45 2012.3

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(NPO)日本歯周病学会

塩基性線維芽細胞増殖因子(FGF-2:basic fibroblast growth factor)は、歯周組織再生誘導薬の有力な候補の一つと期待されており、動物実験および臨床治験によって、その有効性と安全性が明らかにされている。本研究では、KCB-1D(歯周病を対象とした遺伝子組み換えヒト型FGF-2の治験薬コード)を用いた探索的第II相臨床治験に参加した79名の被験者を対象として、フラップ手術時にプラセボ(0%)あるいは0.03%、0.1%、0.3%の何れか濃度のFGF-2を投与した被験歯の長期経過を調査した。すなわち、診療録などの診療情報から、臨床治験最終観察日から本研究調査実施日までの間(約8年間)に、各種濃度のFGF-2あるいはプラセボを投与された被験歯に対して行なわれた治療や、被験歯に出現した症状の内容と年月日を調査した。そして、これらのうち、治験薬投与部位における歯周炎の悪化に起因すると判定された治療や症状をイベント、それ以外を打ち切りとして、生存時間解析を行った。その結果、発生した全イベントは14例で、生存時間解析の結果、0.3%FGF-2投与群はフラップ手術を単独で施行したプラセボ群に比べてイベント発生までの期間の有意な延長が認められた(一般化Wilcoxon検定:p=0.0345)。また、本研究の観察期間を含めてFGF-2投与の安全性に関する問題は認めなかった。(著者抄録)

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Influence of resin coating materials on Porphyromonas gingivalis attachment Reviewed

Ai KUMADA, Yoshizo MATSUKA, Atsushi MINE, Mitsuaki ONO, Junji UEHARA, Norihiro SONOI, Takashi ITO, Shogo TAKASHIBA, Takuo KUBOKI

Dental Materials Journal 31 ( 1 ) 86 - 91 2012

Outer membrane vesicles of porphyromonas gingivalis elicit a mucosal immune response Reviewed

Nakao, R., Hasegawa, H., Ochiai, K., Takashiba, S., Ainai, A., Ohnishi, M., Watanabe, H., Senpuku, H.

PLoS ONE 6 ( 10 ) e26163 - e26163 2011.10

Bacterial substitution of coagulase-negative staphylococci for streptococci on the oral mucosa after hematopoietic cell transplantation Reviewed

Soga Yoshihiko, Maeda Yoshinobu, Ishimaru Fumihiko, Tanimoto Mitsune, Maeda Hiroshi, Nishimura Fusanori, Takashiba Shogo

Supportive Care in Cancer 19 ( 7 ) 995 - 1000 2011.7

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

DOI： 10.1007/s00520-010-0923-9

Other Link： http://link.springer.com/article/10.1007/s00520-010-0923-9/fulltext.html

Chronic periodontitis with multiple risk factor syndrome: a case report. Reviewed International journal

Shimoe M, Yamamoto T, Iwamoto Y, Shiomi N, Maeda H, Nishimura F, Takashiba S

Journal of the International Academy of Periodontology 13 ( 2 ) 40 - 47 2011.7

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：2

OBJECTIVE: Multiple risk factor syndrome is a clustering of cardiovascular risk factors, such as diabetes, dyslipidemia, hypertension, and obesity associated epidemiologically with insulin resistance. This report describes the clinical course of a patient suffering from severe periodontitis with multiple risk factor syndrome, and discusses the association between periodontal infection and systemic health. METHODS: The patient had a history of type 2 diabetes, dyslipidemia, and hypertension for over 10 years. At baseline, her hemoglobin A1 c was 8.1%. However, she had no diabetic complications except periodontitis. The IgG antibody titers against Porphyromonas gingivalis FDC 381 and SU63 were elevated above the mean of healthy subjects +2 standard deviations. Intensive periodontal treatment, including periodontal surgery, was performed to reduce periodontal infection and bacteremia. Her systemic and periodontal conditions were evaluated longitudinally for 10 years. RESULTS: Following periodontal treatment, antibody titers against Porphyromonas gingivalis and hemoglobin A1c values were significantly improved. The other clinical data and medication for her systemic condition also remained stable during supportive periodontal therapy. However, she developed myocardial infarction, and showed continuous deterioration of hemoglobin A1 c level and periodontitis. CONCLUSION: The long-term clustering of risk factors, such as diabetes, dyslipidemia, hypertension, and periodontitis, are associated with the development of myocardial infarction. Treatment of systemic conditions in combination with comprehensive periodontal treatment is important in management of patients with multiple risk factor syndrome.

PubMed

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Specific in situ visualization of plasma cells producing antibodies against porphyromonas gingivalis in gingival radicular cyst: Application of the enzyme-labeled antigen method Reviewed

Tsuge, S., Mizutani, Y., Matsuoka, K., Sawasaki, T., Endo, Y., Naruishi, K., Maeda, H., Takashiba, S., Shiogama, K., Inada, K., Tsutsumi, Y.

Journal of Histochemistry and Cytochemistry 59 ( 7 ) 673 - 689 2011.7

Language：English Publishing type：Research paper (scientific journal) Publisher：SAGE Publications

The enzyme-labeled antigen method was applied to visualize plasma cells producing antibodies to Porphyromonas gingivalis, flora of the human oral cavity. Antibodies to P. gingivalis have reportedly been detected in sera of patients with periodontitis. Biotinylated bacterial antigens, Ag53, and four gingipain domains (Arg-pro, Arg-hgp, Lys-pro, and Lys-hgp) were prepared by the cell-free protein synthesis system using the wheat germ extract. In paraformaldehyde-fixed frozen sections of rat lymph nodes experimentally immunized with Ag53-positive and Ag53-negative P. gingivalis, plasma cells were labeled with biotinylated Arg-hgp and Lys-hgp. Antibodies to Ag53 were detected only in the nodes immunized with Ag53-positive bacteria. In two of eight lesions of gingival radicular cyst with inflammatory infiltration, CD138-positive plasma cells in frozen sections were signalized for Arg-hgp and Lys-hgp. An absorption study using unlabeled antigens confirmed the specificity of staining. The AlphaScreen method identified the same-type antibodies in tissue extracts but not in sera. Antibodies to Ag53, Arg-pro, and Lys-pro were undetectable. In two cases, serum antibodies to Arg-hgp and Lys-hgp were AlphaScreen positive, whereas plasma cells were scarcely observed within the lesions. These findings indicate the validity of the enzyme-labeled antigen method. This is the very first application of this novel histochemical technique to human clinical samples.

DOI： 10.1369/0022155411408906

Other Link： http://journals.sagepub.com/doi/full-xml/10.1369/0022155411408906

Discovery of a patient with strongly suspected bullous pemphigoid in a ward by oral health care providers Reviewed

Kanda, N, Soga, Y, Meguro, M, Tanabe, A, Yagi, Y, Himuro, Y, Fujiwara, Y, Takashiba, S, Kobayashi, N

International Journal of Dental Hygiene 9 ( 2 ) 159 - 162 2011.5

IMMUNE RESPONSES TO PORPHYROMONAS GINGIVALIS INFECTION SUPPRESS SYSTEMIC INFLAMMATORY RESPONSE IN EXPERIMENTAL MURINE MODEL Reviewed

K. Naruishi, K. Omori, H. Maeda, N. Sonoi, K. Funakoshi, K. Hirai, M. Ishii, K. Kubo, H. Kobayashi, T. Tomiyama, D. Yamamoto, I. Tanimoto, K. Kunimatsu, S. Takashiba

JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS 25 ( 2 ) 195 - 202 2011.4

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：BIOLIFE SAS

Periodontitis is localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (T: gingivalis), and the severity correlates to significance of immune responses. Recently, it has been reported that periodontitis is associated with the development of systemic disease such as diabetes and atherosclerosis because of increasing invasion of oral pathogens to the circulation. However, the association between local and systemic infectious responses is still unclear. In the present study, we examined the differences of biological responses in animals with or without bacterial infection. After Balb/c mice were infected subcutaneously with live P gingivalis W83, serum, skin and liver were collected according to experimental protocol. The skin and liver tissues were observed pathologically by haematoxylin-eosin staining, and serum IL-6 levels were measured using ELISA method. Throughout the experimental period, conditions of the mice were observed continuously. As expected, severe infiltration of leukocytes were observed at inflamed skin corresponding to the number of bacterial challenges. Although no inflammatory appearance of skin was observed, serum IL-6 levels were increased dramatically (P < 0.01, Student's t-test) and liver tissues were injured in the mice without bacterial challenge. Interestingly, although severe inflammatory appearance of the skin was observed, serum IL-6 levels were not increased and no inflammatory responses were observed in the liver of the 3-times bacterially challenged group. Importantly, immunoglobulin G against P gingivalis W83 was detected in the blood of mice with 3-times bacterial challenge corresponding to improvement of weight loss and survival. In conclusion, although multiple infections develop severe localized inflammation, the immune system should be sufficient to protect the systemic inflammatory responses.

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