Faculty Profiles - TAKASHIBA Shogo

Rapid fluorometric quantificaition of monocyte attachment in tissue culture wells Reviewed International journal

Lior Shapira, Shogo Takashiba, John R. Kalmar, Thomas E. Van Dyke, Vivian Barak, W. Aubrey Soskolne

Journal of Immunological Methods 165 ( 1 ) 93 - 98 1993.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

A simple fluorometric assay that permits rapid quantification of attachment of monocytes or macrophages in tissue culture wells is described. Using 4,6-diamidino-2-phenylindole (DAPI) as a specific fluorochrome marker for DNA, we observed a dose-dependent increase with strong linear correlation in fluorescent emission over a broad range of DNA concentrations. Measurements of the DNA content of the human monocytic cell line THP-1 demonstrated a linear correlation between fluorescence intensity and cell number from 5 x 10(4) to 1 x 10(6) cells, with an estimated average DNA content of 7.5 pg DNA per cell. While untreated THP-1 cells were not detectably adherent, PMA induction for 24 h results in 57-76% adherence to plastic surface. This method was found to be useful for measuring the number of peripheral blood monocytes separated from lymphocytes by attachment. 16 subjects were sampled and the standard deviation of each individual did not exceed 10%. The number of attached cells was between 10-16% of the total mononuclear cells. Fluorescence measurement of DNA with DAPI permits rapid and accurate determination of cell numbers and appears useful in the quantification of adherent populations such as myelocytic cells and cell lines.

DOI： 10.1016/0022-1759(93)90110-s

歯周病患者におけるHLA-DQB1遺伝子変異の検索 Reviewed

大山秀樹, 高柴正悟, 宮本学, 高橋慶壮, 河野隆幸, 永井淳, 栗原英見, 村山洋二

日本歯周病学会会誌 35 ( 3 ) 536 - 542 1993.9

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(NPO)日本歯周病学会

歯周病患者を含む被験者についてHLA-DQB1遺伝子上のある遺伝子の変異を検索した。1)HLA-DQB1遺伝子の変異の検出頻度は早期発症型歯周炎患者で,成人性歯周炎患者および健常者に比べ有意に高かった。2)家系調査により,この遺伝子変異は突然変異ではなく遺伝性のものであることが示唆された

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1993&ichushi_jid=J01095&link_issn=&doc_id=19940036950010&doc_link_id=%2Fcp9perlo%2F1993%2F003503%2F010%2F0536-0542%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcp9perlo%2F1993%2F003503%2F010%2F0536-0542%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

Host Defense Findings in a Single Family Manifesting Early-Onset Periodontitis Reviewed

CHIHARA Toshihiro, NAGAI Atsushi, AKUTSU Isao, HONGYO Hiroshi, TAKAHASHI Keiso, SHIMIZU Naoko, TANIMOTO Ichiro, SHIMABUKU Osamu, FUJITA Naoko, MIYAMOTO Manabu, TAKASHIBA Shogo, GOTO Hiroyuki, NISHIMURA Fusanori, ISOSHIMA Osamu, SHIMIZU Hideki, KURIHARA Hidemi, NOMURA Yoshio, MURAYAMA Yoji

Journal of the Japanese Association of Periodontology 34 ( 1 ) 204 - 212 1992.3

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Periodontology

Assessments of host defense functions were made in a woman and her two daughters, all of whom manifested early-onset periodontitis (rapidly progressive periodontitis in the mother; localized juvenile periodontitis in the elder daughter; gingivitis in the younger daughter). The analyses of peripheral neutrophil functions revealed depressed neutrophil chemotaxis in the mother. Phenotypic and functional analyses of peripheral lymphocytes showed an elevated percentage of CD 4^+ cells and T 4/T 8 ratios in all subjects, and a depressed lymphocyte proliferative response to stimulation with CD 3 antibody in the younger daughter. Serological typing of HLA antigens revealed that the mother and one of the daughters had some class II HLA phenotypes in common. Serum IgG antibody levels to Actinobacillus actinomycetemcomitans were elevated in all subjects, those to Porphyromonas gingivalis in the mother and younger daughter, and those to Fusobacterium nucleatum in the mother. The pathogenesis of periodontal disease in individual members of this family could not be clarified solely from the results of the host defense functions examined. Other factors in the host defense network must be taken into consideration.

DOI： 10.2329/perio.34.204

Studies on the Status of Periodontal Disease from the Lymphocytes Functions Concerned with Immunoglobulin Synthesis Reviewed

Other Link： http://search.jamas.or.jp/link/ui/1993025574

TAKAHASHI Keiso, NAGAI Atsushi, AKUTSU Isao, SATO Nobuhiko, TAKASHIBA Shogo, MIYAMOTO Manabu, TAKIGAWA Masayuki, MYOKAI Fumio, KATSURAGI Kyoko, HASHIMOTO Toshiaki, NOMURA Yoshio, MURAYAMA Yoji

Journal of the Japanese Association of Periodontology 33 ( 3 ) 685 - 694 1991.9

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Periodontology

The purpose of these studies was to assess the relationship between factors in the immune network concerned with immunoglobulin synthesis. We examined immunoglobulin production, lymphocyte subset ratios and lymphocyte function using peripheral blood mononuclear cells from 50 subjects consisting of 32 patients with periodontal disease and 18 periodontally healthy subjects. On the basis of serum antibody titers to periodontal bacteria, patients were classified into two groups characterized by either low or high titers. Patients had lower proportional counts of suppressor/cytotoxic T cells and higher immunoglobulin productivity than healthy subjects. Subjects with an elevated or decreased ratio of lymphocyte subsets and lymphocyte functions were detected more frequently among patients, irrespective of antibody titers. However, a wide distribution of values was observed among subjects in all of the examination. Based on the results obtained, it appears that lymphocyte functions concerned with humoral immune response vary widely among subjects, and each feature must be clarified individually.

DOI： 10.2329/perio.33.685

Other Link： http://search.jamas.or.jp/link/ui/1993066054

A Family Study of a Mother and Daughter with Increased Susceptibility to Early-Onset Periodontitis: Microbiological, Immunological, Host Defensive, and Genetic Analyses Reviewed International journal

Fusanori Nishimura, Atsushi Nagai, Keiji Kurimoto, Osama Isoshima, Shogo Takashiba, Mitsuharu Kobayashi, Isao Akutsu, Hidemi Kurihara, Yoshio Nomura, Yoji Murayama, Hiroyuki Ohta, Keijiro Kato

Journal of Periodontology 61 ( 12 ) 755 - 765 1990.12

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Microbiological, immunological, host-defensive, and genetic analyses were performed on a mother and daughter, both of whom had early-onset periodontitis (rapidly progressive periodontitis in the mother; localized juvenile periodontitis in the daughter). Microscopic examination revealed a greatly elevated percentage of rod-form bacteria in both subjects. Fusobacterium sp. and Porphyromonas gingivalis (formerly Bacteroides gingivalis) were the predominant microorganisms cultured. The humoral immune responses to F. nucleatum, P. gingivalis, and Actinobacillus actinomycetemcomitans were much higher in both subjects than those to any other periodontal bacteria examined. Functional and phenotypic analysis of the peripheral lymphocytes showed no significant abnormalities. However, investigation of neutrophil function showed that the mother had depressed neutrophil chemotaxis and superoxide production. The daughter had depression not only of chemotaxis and superoxide production, but also of neutrophil phagocytosis. Serological typing of HLA antigens revealed the same Class II HLA profile in both subjects. It was concluded that both subjects very probably had an identical condition and that these patients provided a unique model for improving our understanding of the host factors involved in periodontal disease.

DOI： 10.1902/jop.1990.61.12.755

Web of Science

PubMed

ヒト白血球抗原(HLA)遺伝子の制限断片長多型(RFLP)解析による歯周病病態の分子生物学的研究 Reviewed

高柴正悟

日本歯周病学会会誌 32 ( 2 ) 386 - 401 1990.6

Authorship：Lead author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(NPO)日本歯周病学会

免疫応答に重要なHLAクラス2 β鎖遺伝子の多型解析で遺伝子変異を検出し,それが特定の歯周病のマーカーとなる可能性を探求することを目的とした.日本人の歯周病患者70名および歯周組織が健康な26名のHLAフェノタイプを補体依存性細胞障害試験で同定し,HLAフェノタイプの検出頻度を調べたが患者に特徴的なHLAフェノタイプは見出せなかった.HLAの微細な差異を検出するため,歯周病患者15名および健康者5名について,HLAクラス2 β鎖遺伝子のRFLP解析を行なった.一部の患者に特徴的なHLA-DQ β鎖遺伝子の変異を検出した.また,ポリメラーゼチェインリアクション法を用いて増幅したDNAを用いることによっても,その変異を検出できた

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1990&ichushi_jid=J01095&link_issn=&doc_id=19870249440004&doc_link_id=%2Fcp9perlo%2F1990%2F003202%2F004%2F0386-0401%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcp9perlo%2F1990%2F003202%2F004%2F0386-0401%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

Application of synthetic hydroxyapatite to periodontal therapy Reviewed

Morita, K., Ebisu, S., Toda, H., Shimabukuro, Y., Okada, H., Gotou, H., Nishimura, F., Nakamura, T., Takashiba, S., Nomura, Y.

Nippon Shishubyo Gakkai kaishi 31 ( 1 ) 1989

Periodontal Therapy by Local Delivery of Minocycline : Clinical Study of Periodontal Therapy by LS-007 Reviewed

KURIMOTO Keiji, ISOSHIMA Osamu, NAORA Yuka, ANADA Takashi, KOBAYASHI Yoshitomo, KOBAYASHI Mitsuharu, ARAI Hideo, TAKASHIBA Shogo, NANBA Hideki, YOKOYAMA Masayuki, MITSUDA Yuka, MIZUSHIMA Yumi, NOMURA Yoshio, MURAYAMA Yoji, UEDA Masatoshi, TERANISHI Yoshihiro, FUJIWARA Kazuyuki, HASHIZUME Akiko, KAMAYA Shinpei, HOSOYAMA Yoko, UEBA Kenji, ONISHI Kazuyuki, SHIRAI Takeo, OHASHI Satoshi, HIGASHI Hirosuke, KIOKA Yoshifumi, MINAMIBAYASHI Shigeyoshi, TANAKA Mayumi, KITAMURA Takuya, MAKIGUSA Kazuto, YAMAOKA Akira, URAGUCHI Ryoji, HAGIWARA Satsuki, FUKUDA Mitsuo, ODA Sigeru, LIN Cherng-Jong, TAKEFUTA Wataru, MERA Toyotsune, MINEGISHI Daizou, UMEDA Makoto, NAKAMOTO Hiroshi, INATOMI Hirofumi, Narongsak Laosrisin, NOGUCHI Toshihide, ISHIKAWA Isao

Journal of the Japanese Association of Periodontology 30 ( 1 ) 191 - 205 1988.3

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Periodontology

Our previous studies have been performed to establish methods for local delivery of Minocycline hydrochloride (MINO: Lederle Japan, LTD, Tokyo) in a therapy for periodontal disease. This study was done clinically to evaluate the effectiveness, safety, and usefulness of a LS-007 medicine containing 2 percent titers of MINO. Forty five periodontitis patients (119 teeth) with periodontal pockets (≧4mm) participated in this study. The experimental systems were evaluated by comparing microbiological and clinical effectiveness of LS-007, placebo, and Minocycline tablet (Lederle Japan, LTD). The results were as follows, 1. The effectiveness of LS-007 was examined in two delivery system; in one system the medicine was redelivered after one week, and in the other system after two weeks. Both systems revealed similar effectiveness until after two weeks from the last delivery. 2. When LS-007 was delivered once in a week for 4 successive weeks, LS-007 demonstrated its effectiveness until after 4 weeks from the last delivery. 3. A pretreatment with scaling prior to the application of LS-007 raised the effectiveness of LS-007. 4. The systemic delivery of Minomycine tablet demonstrated microbiologically and clinically similar effect to the local delivery of LS-007. In the systemic delivery, however, there was a subject who suffered harmful side effect of the medicine. From these results, we concluded the local delivery of LS-007 was clinically effective, safe, and useful as a therapy for periodontal disease.

DOI： 10.2329/perio.30.191

Studies on Microflora and Host Defensive Function of a Mother and Daughter with Early Onset Periodontitis Reviewed

Other Link： http://search.jamas.or.jp/link/ui/1989131026

POLYMORPHISM OF COMPROMISED HOSTS IN PERIODONTITIS PATIENTS Reviewed

S TAKASHIBA, F NISHIMURA, M KOBAYASHI, A NAGAI, AKUTSU, I, K OKAMURA, O ISOSHIMA, H KURIHARA, Y NOMURA, Y MURAYAMA

RECENT ADVANCES IN CLINICAL PERIODONTOLOGY 790 383 - 386 1988

NISHIMURA Fusanori, NAGAI Atsushi, OKAMURA Kazunori, KURIMOTO Keiji, ISOSHIMA Osamu, NAORA Yuka, KUMAZAWA Hiroshi, SUGIYAMA Masaaki, ANADA Takashi, SHIMIZU Hideki, NAKAMURA Tetsuya, KOBAYASHI Yoshitomo, KOBAYASHI Mitsuharu, TAKASHIBA Shogo, MIZUSHIMA Yumi, MITSUDA Yuka, YOKOYAMA Masayuki, KURIHARA Hidemi, KINOSHITA Masahiko, NOMURA Yoshio, MURAYAMA Yoji, OHTA Hiroyuki, KATO Keijiro

Journal of the Japanese Association of Periodontology 29 ( 2 ) 492 - 505 1987.6

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Periodontology

Early-onset periodontitis has been implicated from the perspective of a host-parasite interaction. This case report describes microbiological examinations and the assessment of host defence function which were performed on a mother and daughter both of whom had early-onset periodontitis. The mother's diagnosis was rapidly progressive periodontitis. She had severe gingival inflammation and progressive bone destraction. The daughter's diagnosis was localized juvenile periodontitis. She had little gingival inflammation, but presented with progressive bone resorption. Microbiological examinations revealed an elevated proportion of rods and spirochetes from the mother's affected sites. Fewer spirochetes were found in the daughter's affected sites. Fusobacterium sp. were the predominant microorganisms in the plaque of both the mother and the daughter. Actinobacillus actinomycetemcomitans was not detected in the subgingival plaque of either the mother or the daughter. Humoral immune responces both in mother and daughter were much higher to F. nucleatum B. gingival, and A. actinomycetemcomitans than any other periodontally related microorganisms examined. Peripheral neutrophil functions were also studied. These studies demonstrated that the mother had a depressive neutrophil phagocytosis. The neutrophil studies performed for the daughter revealed a depressed function not only in neutrophil phagocytosis but also in neutrophil chemotaxis. From the perspective of host-parasite interaction, we propose that the disease status of the mother and the daughter are very similar. We believe the disease process in both the mother and the daughter strongly suggests an identical mechanism.

DOI： 10.2329/perio.29.492

Serum Antibodies of Periodontaly Related Microorganisms : Changes of the IgG Antibodies Following Periodontal Treatment Reviewed

Other Link： http://search.jamas.or.jp/link/ui/1988053904

OKAMURA Kazunori, NAGAI Atsushi, KUMAZAWA Hiroshi, SUGIYAMA Masaaki, MIZUSHIMA Yumi, MITSUDA Yuka, TAKASHIBA Shogo, KURIHARA Hidemi, NOMURA Yoshio, MURAYAMA Yoji

Journal of the Japanese Association of Periodontology 29 ( 1 ) 146 - 154 1987.3

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Periodontology

Several studies in serum antibodies of putative periodontopathic microorganisms have reported a correlation between specific antibacterial titers and the various forms of periodontal disease. In this report, effect of periodontal treatment on the levels of the IgG antibodies to Actinobacillus actinomycetemcomitans (Aa), Bacteroides gingivalis (Bg) and Capnocytophaga ochracea (Co) was evaluated. Sera were obtained from patients with periodontal diseases following periodontal treatment. The serum IgG antibody titers were assessed with an enzyme-linked immunosorbent assay (ELlSA). The antibody levels were compared before and after treatment. The post-treatment levels of the IgG antibodies to Aa, Bg and Co decreased significantly from the pre-treatment levels. The subjects were devided into two groups, a moderately improved group and a completely improved group. Decreases in the antibody levels were consistently found in the later group and not in the former group. In the completely improved group 20 of 23 patients (81%) ; 21 of 26 (87%); and 13 of 13 patients (100%) had decreased antibody levels to Aa, Bg and Co, respectively. A significantly decreased antibody level was defined as more than 20 per cent levels below the corresponding pre-treatment level. The IgG immune response differed according to the lapse of time after surgical treatments including scaling, root planing and peridontal surgery. The IgG antibody levels to Co decreased significantly in the early stages (within 30 days) after surgical treatment, in contrast those with Aa and Bg increased a little with no significant differences in the early stages. However, 30 days following after surgical treatment, those with Aa and Bg also decreased significantly. Surgical treatment is considered to boost the humoral immune response to the microorganisms. Generally, the IgG response elevated for 30 days or more after antigen exposure with the booster.

DOI： 10.2329/perio.29.146