Faculty Profiles - TAKASHIBA Shogo

Papers - TAKASHIBA Shogo

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Unique genes induced by mechanical stress in periodontal ligament cells Reviewed International journal

Fumio Myokai, Masataka Oyama, Fusanori Nishimura, Taisuke Ohira, Tadashi Yamamoto, Hideo Arai, Shogo Takashiba, Yoji Murayama

Journal of Periodontal Research 38 ( 3 ) 255 - 261 2003.6

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Objectives: The aim of this study is to isolate mechanical stress-induced genes (MSGens) from human periodontal ligament (PDL) cells and to analyze profiles of the mRNA expression of these genes.
Background: Differential expression of genes in PDL cells under physiological stress such as occlusal force is thought to be orchestrated not only for the remodeling of PDL itself but also for the repair and regeneration of periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress.
Methods: The cDNA from mechanical stress-applied human PDL cells was subtracted against the cDNA from static control cells. The subtracted cDNA was amplified by polymerase chain reaction (PCR) and cloned for further analysis.
Results: Among 68 independent clones isolated, 15 contained DNA fragments greater than 250 bp. Reverse Northern analysis revealed a marked induction of MSGen-15 and MSGen-28 mRNA expression in the mechanical stress-applied cells. However, little difference in the magnitude of expression for the other MSGens was detected between the stress-applied cells and the control cells. After nucleotide sequencing and the analysis of homology with known genes, five clones were identified; ribosomal protein S27 (MSGen-9), MRG 15 (MSGen-15), androgen-binding protein (MSGen-18), cathepsin H (MSGen-28), and cytochrome c (MSGen-47). Interestingly, it has been reported that MRG 15 is a novel transcription factor involved in the regulation of cell growth and senescence. The remaining 10 clones, classified into six sequence types, had no significant homology with any known genes.
Conclusions: These results suggest that many known and unknown genes are expressed in response to mechanical stress in PDL cells, and that a transcription factor, MRG 15, may be responsible for molecular events in PDL cells under mechanical stress.

DOI： 10.1034/j.1600-0765.2003.00602.x

Web of Science

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Ligation of IFN-γ-induced HLA-DR molecules on fibroblasts induces RANTES expression via c-Jun N-terminal kinase (JNK) pathway Reviewed

Meguro, M., Nishimura, F., Ohyama, H., Takashiba, S., Murayama, Y., Matsushita, S.

Cytokine 22 ( 5 ) 107 - 115 2003.6

Antibacterial activity of synthetic human B defensin-2 against periodontal bacteria. Reviewed International journal

Mineshiba F, Takashiba S, Mineshiba J, Matsuura K, Kokeguchi S, Murayama Y

Journal of the International Academy of Periodontology 5 ( 2 ) 35 - 40 2003.4

Language：English Publishing type：Research paper (scientific journal) Publisher：2

The oral epithelium is continuously exposed to a variety of microbial challenges that can cause infectious diseases such as periodontal disease. Human B Defensin-2 (hBD-2) is a cationic antimicrobial peptide with low molecular weight, which is inducible from oral epithelial cells upon either bacterial infection or stimulation with inflammatory cytokines. This peptide has a broad antimicrobial spectrum that includes gram-positive bacteria, gram-negative bacteria, and fungi. Therefore, it is thought that hBD-2 plays an important role as one of natural immunities to bacterial infection. However, its activity is inhibited by body fluids such as serum. The aim of this study was to assess the antibacterial activity of synthetic hBD-2 against oral bacteria in the presence of saliva or serum. The antibacterial activity of synthetic hBD-2 was tested against Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans, and Escherichia coli. Antibacterial broth assay and diffusion assay were performed in vitro. The antibacterial activity of hBD-2 was approximately equal to that of minocycline at equimolar concentrations. Furthermore, the activity of hBD-2 remained at 60% in the presence of 80% saliva, whereas no activity remained in the presence of 20% serum. Our results suggest the possibility that synthetic hBD-2 could be useful to prevent infection by periodontal bacteria.

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歯周治療により炎症マーカーが改善したと考察された重度歯周炎を伴うリウマチ患者の症例報告 Reviewed

工藤値英子, 河野隆幸, 西村英紀, 大山秀樹, 明貝文夫, 成石浩司, 岩本義博, 高橋直樹, 曽我賢彦, 新井英雄, 村山洋二, 高柴正悟

日本歯科保存学雑誌 46 ( 1 ) 110 - 117 2003.2

Authorship：Last author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(NPO)日本歯科保存学会

慢性関節リウマチ(RA)患者の歯周治療を取り上げ,歯周治療がRAの臨床データへ及ぼす影響について考察した.50歳女.下顎右側第二大臼歯に自発痛及び咬合痛を自覚し,修復処置をうけた.その後,症状があまり改善しないため受診した.又,患者はRAの治療のため本歯周治療に伴い,歯周病原性細菌に対する血清IgG抗体価が低下した.血清IgG抗体価の低下に呼応して,TNF-αとCRP値が著明に低下した.よって,RAを伴う重度歯周炎に対して徹底した歯周処置を行うことによって,末梢血レベルでの炎症マーカーを改善することができる可能性が示唆された

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コンピューターグラフィックスを応用した歯周・歯内病態の生物学的な教育 Reviewed

新井英雄, 北中通誉, 河野隆幸, 前田博史, 鷲尾憲文, 大山秀樹, 明貝文夫, 谷本一郎, 千原敏裕, 大江丙午, 小柳津功介, 西村英紀, 高柴正悟, 村山洋二

日本歯科保存学雑誌 45 ( 5 ) 808 - 816 2002.10

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：(NPO)日本歯科保存学会

歯内・歯周疾患の病態と治癒に関わる生物学的な理解を深める為に,コンピューターグラフィックス教材を開発して講義に使用して有用性を評価することを企画した.評価方法としてコンピューターグラフィックスを利用した講義に対する学生の評価を基として学生へのアンケート形式をとった.コンピューター技術を活用して臨床教育の合理化・能率化を図ると共に,インターネットを学生との対話を緊密化する手段として利用でき,グループ学習によるチュートリアル教育を取り入れ,個体医療やEvidence based medicine;EBMに基づいた医療に対応できる歯科医師の養成を図るとことも可能である.5年次・6年次学生を対象にその画像を用いて講義を行った結果,学生への歯科保存学臨床教育において有用であると評価できた

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歯周病のオーダーメイド治療に向けた単球機能の分子生物学的研究 Invited Reviewed

高柴正悟

日本歯周病学会会誌 44 ( 3 ) 254 - 260 2002.9

非吸収性,吸収性メンブレンを用いた歯周組織再生誘導(Guided Tissue Regeneration)法の評価 Reviewed

河野隆幸, 峯柴淳二, 清水明美, 澤田聡子, 峯柴史, 大山秀樹, 尾山正高, 澤田弘一, 新井英雄, 西村英紀, 高柴正悟, 村山洋二

岡山歯学会雑誌 21 ( 1 ) 39 - 47 2002.6

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：岡山歯学会

辺縁性歯周炎患者38名を対象に非吸収性・吸収性メンブレンを用いたGTR法を行い,その評価と予後に影響する因子について検討した.その結果,GTR前後におけるプロービング深さ(PD)とクリニカルアタッチメント(CAL)の変化は,術前ではPD:6.55±1.45mm,CAL:8.61±2.04mm,GTR後ではPD:3.13±0.96mm,CAL:5.84±2.07mmと,術前と比べ術後にPDとCALの有意な減少を認めた.又,骨欠損の形態の違いによる比較では,3壁性骨欠損は2級根分岐部病変と比べ,術後PD値の減少量が有意に大きかった.術前PDの違いによる比較では,術前PD値7mm以上の症例が6mm以下の症例と比べ,術後のPD値の減少量とCALの獲得量が有意に大きかった.以上より,GTR法はPDの減少とCALの獲得において有用な術式であることが示唆された.又,GTR法の予後に強く影響を与える因子は,骨欠損の形態と術前のPDであることが明らかとなった

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Regulation of Vitamin D_3 Receptor Gene Expression by Human Periodontal Ligament Fibroblasts Reviewed

WASHIO Norifumi, ARAI Hideo, MYOKAI Fumio, NISHIMURA Fusanori, TAKASHIBA Shogo, NAGAI Atsushi, MURAYAMA Yoji

38 117 - 120 2002.3

COUNTER-ANTIGEN PRESENTATION: FIBROBLASTS PRODUCE CYTOKINES BY SIGNALLING THROUGH HLA CLASS II MOLECULES WITHOUT INDUCING T-CELL PROLIFERATION Reviewed

Hideki Ohyama, Fusanori Nishimura, Michio Meguro, Shogo Takashiba, Yoji Murayama, Sho Matsushita

Cytokine 17 ( 4 ) 175 - 181 2002.2

歯周病原性細菌に対する血清IgG抗体を測定することによって集団検診で若年性歯周炎患者を検出する方法に関する研究 Reviewed

大山秀樹, 岡本慎治, 西村英紀, 新井英雄, 高柴正悟, 村山洋二

岡山歯学会雑誌 20 ( 2 ) 181 - 191 2001.12

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：岡山歯学会

28名の歯学部学生に対してA.actinomycetemcomitans及びP.gingivalisに対する血清IgG抗体を測定し,その双方に対して高い抗体値を示す集団を特定し,更にその中から1名の若年性歯周病患者を見出すに至った.この方法は,集団検診における一次スクリーニングとして有益な方法であることが明らかとなった

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ハンセン病患者の歯周病の臨床症状と歯周病関連細菌に対する体液性免疫応答に関する疫学的研究 Reviewed

大山秀樹, 本行博, 清水尚子, 清水良和, 永井淳, 野村慶雄, 西村英紀, 新井英雄, 高柴正悟, 村山洋二

岡山歯学会雑誌 20 ( 2 ) 193 - 200 2001.12

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：岡山歯学会

439名のハンセン病患者の歯周病の実態を歯周病の臨床症状及び歯周病原性細菌に対する体液性免疫応答から調べた.その結果,臨床症状からはハンセン病既往者集団の特異性を明らかにすることはできなかった.しかし,体液性免疫応答は歯周病原性細菌のある菌種に対して亢進していることが明らかとなった

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T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy Reviewed

Hideki Ohyama, Sho Matsushita, Fusanori Nishimura, Nahoko Kato, Kentaro Hatano, Shogo Takashiba, Yoji Murayama

Vaccine 20 ( 3-4 ) 475 - 482 2001.11

Subgingival microflora and antibody responses against periodontal bacteria of young Japanese patients with type 1 diabetes mellitus. Reviewed International journal

Takahashi K, Nishimura F, Kurihara M, Iwamoto Y, Takashiba S, Miyata T, Murayama Y

Journal of the International Academy of Periodontology 3 ( 4 ) 104 - 111 2001.10

Language：English Publishing type：Research paper (scientific journal) Publisher：4

Periodontal disease is a complication of patients with type 1 diabetes mellitus (T1DM), although the mechanisms responsible for this relationship remain unclear. The aim of this study was to examine oral manifestations and the prevalence of periodontal pathogens from subgingival plaque samples and serum IgG antibody levels against them in young Japanese type 1 diabetic subjects. One hundred and seventeen Japanese T1DM subjects (53 male, 64 female, mean age +/- SD, 16 +/- 6.5 years) participated in this study. Thirty-nine periodontally healthy, age-matched nondiabetics served as controls. T1DM subjects were clinically assigned into three groups: 12 periodontitis, 32 gingivitis and 73 periodontally healthy. Microbiological tests for four periodontal pathogens, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Prevotella intermedia and Capnocytophaga ochracea were performed using 16S ribosomal RNA-based polymerase chain reaction methods. Serum IgG antibody levels against 12 periodontal bacteria including the four species assessed by polymerase chain reaction were measured by enzyme-linked immunosorbent assay. In the T1DM subjects, the Periodontitis group had a significantly longer mean duration of diabetes and a higher percentages of subjects harbouring P. gingivalis and P. intermedia than the Periodontally Healthy group. Serum IgG antibody levels against P. gingivalis were significantly elevated in the Periodontitis group compared with Gingivitis and Periodontally Healthy groups. These results indicate that Japanese T1DM subjects are a high-risk group for periodontal disease and both P. gingivalis infection and duration of T1DM are risk factors for the progression of periodontitis in patients with T1DM.

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Characterization of two genes encoding ferritin-like protein in Actinobacillus actinomycetemcomitans Reviewed

Hirosue, M., Kokeguchi, S., Maeda, H., Nishimura, F., Takashiba, S., Murayama, Y.

Microbiology and Immunology 45 ( 10 ) 721 - 727 2001.10

0.04%グルコン酸クロルヘキシジン洗口剤の殺菌効果に関する研究 Reviewed

片山知子, 大橋敏雄, 苔口進, 綿城哲二, 弘末勝, 澤田弘一, 澤田聡子, 清水明美, 藤本千代, 杉典子, 川端英二, 高柴正悟, 村山洋二

岡山歯学会雑誌 20 ( 1 ) 129 - 133 2001.6

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：岡山歯学会

医薬部外品として使用できる0.04%グルコン酸クロルヘキシジン水溶液を洗口剤として使用した時の殺菌効果を,唾液中の細菌数の変化を指標として評価し,洗口剤としての有用性を検討した.我が国で洗口に用いることが許可されている0.04%濃度のクロルヘキシジン含有洗口液が唾液中の細菌に対する殺菌効果を有することがわかった.したがって,0.04%濃度であっても,洗口剤として有用であることが示唆された

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Supportive Periodontal Treatment(SPT)期にある歯周病患者の根面う蝕症の実態 Reviewed

清水明美, 大橋敏雄, 山崎太士, 杉典子, 塩見信行, 竹内加珠, 村内利光, 岡本慎治, 前田武将, 窪木拓男, 矢谷博文, 山下敦, 高柴正悟, 村山洋二

岡山歯学会雑誌 20 ( 1 ) 119 - 127 2001.6

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：岡山歯学会

Supportive Periodontal Treatment(SPT)期にある歯周病患者の根面う蝕の実態を報告した.SPT患者群と非SPT患者群間ではう蝕罹患経験,う蝕リスク因子による有意差はなく,う蝕罹患の危険性に差はなかった.露出根面に対してう蝕や修復の多いSPT患者はう蝕リスクが大きく,更にう蝕を引き起こす可能性が高いことが示唆された

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Impairment of Gingival Fibroblast Adherence by IL-6/sIL-6R Reviewed International journal

K. Naruishi, S. Takashiba, F. Nishimura, H.-H. Chou, H. Arai, H. Yamada, Y. Murayama

Journal of Dental Research 80 ( 5 ) 1421 - 1424 2001.5

Language：English Publishing type：Research paper (scientific journal) Publisher：SAGE Publications

Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gpl30 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPβ. Cytoskeletal β-actin and adhesion molecule integrin-a5, a subunit of α₅β₁ integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of (3-actin and integrin-a5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.

DOI： 10.1177/00220345010800050701

Web of Science

PubMed

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Identification and characterization of B-cell epitopes of a 53-kDa outer membrane protein from Porphyromonas gingivalis Reviewed

Oyaizu, K., Ohyama, H., Nishimura, F., Kurihara, H., Matsushita, S., Maeda, H., Kokeguchi, S., Hongyo, H., Takashiba, S., Murayama, Y.

Oral Microbiology and Immunology 16 ( 2 ) 73 - 78 2001.4

Serum phenytoin concentration and IgG antibody titre to periodontal bacteria in patients with phenytoin-induced gingival overgrowth. Reviewed International journal

Yamada H, Nishimura F, Furuno K, Naruishi K, Kobayashi Y, Takashiba S, Murayama Y

Journal of the International Academy of Periodontology 3 ( 2 ) 42 - 47 2001.4

Language：English Publishing type：Research paper (scientific journal) Publisher：2

Epileptic patients taking phenytoin with gingival-overgrowth and those without gingival-overgrowth were compared for daily drug dose, plasma total phenytoin concentration, plasma free-phenytoin concentration and serum IgG antibody titre against 13 periodontal bacteria. Significantly higher daily drug dose was noted in patients with gingival overgrowth (P < 0.05) when compared with those without overgrowth. In addition, both total and free forms of plasma phenytoin concentration were significantly higher in sera of patients with gingival growth than of those without overgrowth (P < 0.01). Strong positive correlation was found between daily drug dose and serum phenytoin concentration in patients with gingival overgrowth, while weak correlation was found in patients without gingival overgrowth, suggesting a difference in drug metabolism in these two groups. However, no differences were found in serum IgG antibody titres to 13 periodontal bacteria examined between two groups. These results suggest that metabolic ability of phenytoin is one of the factors for developing gingival overgrowth, and that periodontal infection may not be a primary causative factor for gingival overgrowth but act as an additive factor which increase tissue mass for this unwanted side effect.

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Heterogeneity of host immunological risk factors in patients with aggressive periodontitis Reviewed

Takahashi, K, Ohyama, H, Kitanaka, M, Sawa, T, Mineshiba, J, Nishimura, F, Arai, H, Takashiba, S, Murayama, Y

Journal of Periodontology 72 ( 4 ) 425 - 437 2001.4

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Papers - TAKASHIBA Shogo