研究者詳細 - 長塚　仁

MISC - 長塚　仁

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Comparative study of heterotopic bone formation induced by BMP in adult and young rats

Chun-Lin Qin, Masaru Murata, Masahisa Inoue, Hitoshi Nagatsuka, Manabu Kanyama, Hironobu Konouchi, Hidetsugu Tsujiguiwa, Noriyuki Nagai, Dept. of Oral Pathology School of Dentistry Okayama Univ., Dept. of Oral Pathology School of Dentistry Okayama Univ., Dept. of Oral Pathology School of Dentistry Okayama Univ., Dept. of Oral Pathology School of Dentistry Okayama Univ., Dept. of Fixed Prosthodontics School of Dentistry Okayama Univ., Dept. of Oral Pathology School of Dentistry Okayama Univ., Dept. of Oral Pathology School of Dentistry Okayama Univ., Dept. of Oral Pathology School of Dentistry Okayama Univ.

Journal of hard tissue biology = Journal of hard tissue biology 4 ( 1 ) 36 - 37 1995年3月

The Effect of Enzymatic Treatment on Immunohistochemical Staining of Collagens in Mandibular Condyle of Rats

Hong-In SHIN, Yuzo ISHIWARI, Hitoshi NAGATSUKA, Masahisa INOUE, Masaru MURATA, Hironobu KONOUCHI, Chun-Lin QIN, Tadao NAKAGO, Yoshiho CHIGONO, Tohru TAKAGI, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Kyungpook National University and Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Orthodontics Okayama University Dental School, Department of Oral Surgery Faculty of Medicine Teikyo University, Department of Biochemistry Faculty of Dentistry Tokyo Medical and Dental University, Department of Oral Pathology Okayama University Dental School

Journal of hard tissue biology 4 ( 1 ) 8 - 14 1995年3月

記述言語：英語

This study was performed to evaluate the effect of enzymatic treatment on immunohistochemical localization of types I and II collagen in mandibular condyle of rats. The 4% paraformaldehyde and 0.1% glutaraldehyde-fixed, paraffin-embedded tissue sections were stained by anti-type I and II collagen antibodies using the PAP method. In order to compare the immunostaining conditions under various enzymatic treatments, the sections were digested with hyaluronidase, pepsin, trypsin, and actinase. The results demonstrate a variety of immunohistochemical reactions for each collagen according to the applied enzymes. The specificity and intensity of immunostaining for typesI and II collagen in the mandibular condyle were more favorable in pepsin treated sections than in other section.

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その他リンク： http://search.jamas.or.jp/link/ui/1997000411

Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization

Noriyuki NAGAI, Hitoshi NAGATSUKA, Masaru MURATA, Masahisa INOUE, Takumi AKAGI, Chun-Lin QIN, Keisuke NAKANO, Yuzo ISIWARI, Hironobu KONOUCHI, Hidetsugu TSUJIGIWA, Yoshiho CHIGONO, Tohru TAKAGI, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Surgery Faculty of Medicine Teikyo University, Department of Biochemistry Tokyo Medical and Dental University Faculty of Dentistry

Journal of hard tissue biology 4 ( 1 ) 15 - 23 1995年3月

記述言語：英語

A sensitive in situ hybridization method with high resolution has been developed usin digoxigenin-II VTP labeled cRNA as a probe. The system was applied to a heterotopic induced bone with crude BMP. The localization of mRNA of the bone matrix proteins, osteopontin (OSP) and osteocalcin (OSC), was examined in the BMP-induced cartilage and bone in comparison with the endochondral ossification. In the heterotopic bone, OSP mRNA were detected in the osteoblast, young osteocyte and chondrocyte-like cells. In fatal endochondral ossification OSP mRNA was detected in the hypertrophic chondrocyte, osteoblast and young osteocyte, while OSC mRNA was detected in the osteoblast and young osteocyte, but hypertrophic chondrocyte was not detected. These results suggest that the chondrocyte-like cells in the BMP induced bone demonstrate the ambifunctions of the chondrocyte and osteoblast. Our results indicate that in situ hybridization immunohistochemistry is useful in identifying the cell phenotypes and their function.

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その他リンク： http://search.jamas.or.jp/link/ui/1997001332

アパタイト-骨結合界面の初期石灰化とBMPによる異所性骨誘導 (<特集>歯科インプラント材料と組織のインターフェイス)

永井教之, 長塚仁, 井上正久, 村田勝, 赤木巧, 辻極秀次, 中野敬介, 馬渕優, 寒河江登志朗, 高木亨, 久保木芳徳

Journal of hard tissue biology = 日本硬組織研究技術学会雑誌 4 ( 3 ) 79 - 90 1995年

BMP誘導異所性硬組織界面の骨芽細胞分化と骨基質蛋白遺伝子の発現 : In Situ Hybridization法による検討

長塚仁, 今村高行, 井上正久, 村田勝, 赤木巧, 石割裕三, 林勝彦, 此内浩信, 申洪仁, 秦春林, MISSANA L., 高木亨, 永井教之

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 3 ( 1 ) 7 - 12 1994年

記述言語：日本語出版者・発行元：日本硬組織研究技術学会

Osteopontin(Osp), osteonectin(Osn),osteocalcin(Osc) gene in new formational bone induced by bone morphogenetic protein(BMP) and tooth germ were examined in this study. BMP-carrier(collagen I) composites were implanted in subcutaneous tissue of the back of rats. At 1,2,3 weeks after implanted, all specimens were immersed in 4% paraformaldehyde and processed for routine light microscopy with or without decalcification. For in situ hybridization, we use single strand RNA probes by DIG RNA Labeling Kit, and stained with Nucleic Acid Detection Kit(Boehringer Mannheim). Osp and Osn signals both appears on the osteoblastic or fibroblastic cells at 1 week after implantation. Osc signal appears on the osteoblasts at 2 weeks after implantation. Osc signal increase on the osteoblasts around mineralized trabecular bone at 3 weeks after implantation. These results suggest that distribution of these bone matrix protein gene was associated with growth and mineralization of bone formation.

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306507

3. マウス下顎頭軟骨におけるコラーゲン遺伝子の発現 : in situ hybridization法による検討

石割裕三, 長塚仁, 井上正久, 村田勝, 今村高行, 永井教之, 阿部信寛, 二宮善文

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 3 ( 1 ) 46 - 46 1994年

Histopathological Studies of Heterotopic Bone Formations Induced by BMP Carrier Composites :

Murata Masaru, Inoue Masahisa, Nagatsuka Hitoshi, Konouchi Hironobu, Qin Chun-Lin, Zhang Shao-Quan, Mizuno Morimichi, Kuboki Yoshinori, Takagi Tohru, Nagai Noriyuki

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 3 ( 2 ) 67 - 72 1994年

記述言語：英語出版者・発行元：日本硬組織研究技術学会

The partially purfied bone morphogenitic protein (BMP) was mixed with insoluble bone matrix (IBM) or porous particles of hydroxyapatite (PPHAP). The BMP composites were implanted into the dorsal subcutaneous tissue of 4 week-old male rats. Each composite was removed after 1, 2 and 3 weeks, and the histological patterns were observed by routine histology. In the BMP-PPHAP system, direct bone formation was observed in a process that resembled an intramembranous ossification. These obseervations indicate that BMP differentiated immature mesenchymal cells tnto osteognic or chondrogenic cells, depending on the physico-chemical nature of hte carrier that influence the tissue microenvironment.

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306544

歯原性腫ようおよび歯はいにおけるテネイシン,フィブロネクチン,IV型コラーゲン局在の免疫組織化学的検索

山近英樹, 西嶋克巳, 日名雅彦, 井上正久, 長塚仁, 永井教之

岡山歯学会雑誌 13 ( 1 ) 1994年

6. DIG標識RNA probeを用いたnon-Ri in situハイブリダイゼーションによる骨基質蛋白mRNAの検出

今村高行, 長塚仁, 井上正久, 永井教之

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 2 ( 1 ) 46 - 46 1993年

30. 歯胚及び歯原性腫瘍のテネイシンの局在に関する免疫組織化学的検討

日名雅彦, 長塚仁, 石割裕三, 林勝彦, 永井教之

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 2 ( 1 ) 51 - 51 1993年

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