研究者詳細 - 長塚　仁

Detection of pheomelanin and eumelanin in lesions of malignant melanomas and melanosis in oral mucosa

Detection of mRNA in hard tissue using in situ hybridization

Ishiwari,Y, Nagatsuka, H, Tsujigiwa, H, Nakano, K, Nagai, N

PROCEEDINGS of Asian Science Seminar in Okayama 2000 2000年

Immunohistochemistry in hard tissue research

Nagatsuka, H, Ishiwari,Y, Nakano, K, Tsujigiwa, H

PROCEEDINGS of Asian Science Seminar in Okayama 2000 2000年

Lee, Y.J, Nagaoka, N, Sakae, T, Takagi, T, Nagatsuka, H, Shinnou, Y, Hibi, K, Nagai, N

PROCEEDINGS of Asian Science Seminar in Okayama 2000 10 ( 2 ) 89 - 95 2000年

記述言語：英語出版者・発行元：日本硬組織研究技術学会

We attempted to elucidate the characteristics of melanin in malignant melanomas in oral mucosa by ultrastructural and energy-filtering transmission electron microscopy to examie the morphology and sulfur contents of the melanins in oral lesions of benign melanosis and malignant melanonmas. In lesions of melanosis of the oral mucosa, the essential pathological features are over-production of eumelanin and migration of melanin into keratinocytes. In lesions of malignant melanoma in situ, abnormal melanosomes consisting of many pleomorphic and ellipsoid forms are present. Sulfur distribution is increased, showing an increase in pheomelanin-like melanin. In invasive malignant melanoma the melanosomes assume irregular ellipsoid and spheroid forms, with the presence of compound melanosomes. The contents are eluted by alkali and most contain sulfur, indicating marked increase of pheomelanin-like melanosomes. The morphology and properties of melanosomes are important differential features for melanocyte-derived melanotic lesions including benign tumor, melanosis, and malignant melanoma. These findings habe great significance in the interpretation of early or precancerous lesios.

14. Distribution pattern of αl(IV)- α6(IV)chains of type IV collagen in tooth germ morphogenesis(8th Annual Meeting of the Society of Hard Tissue Biology July 24, 1999 in Asahikawa)

その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=357319

Noriyuki Nagai, Keisuke Nakano, Yuzo Ishiwari, Hitoshi Nagatsuka, Yoshiko Kitamura, Tohru Takagi, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Biochemistry Faculty of Dentistry of Tokyo Medical and Dental University

Journal of hard tissue biology = Journal of hard tissue biology 9 ( 1 ) 27 - 28 2000年

rhBMP-2による異所性骨形成に及ぼす固定化効果

永井教之, 譚軍, 井上正久, 石割裕三, 長塚仁

歯科基礎医学会雑誌 41 ( 5 ) 504 - 504 1999年8月

Long-term observation of heterotopic bone formation induced by rhBMP-2 and IBM composite.

Inoue, M, Tan, J, Tsujigiwa, H, Kitamura, Y, Hibi, K, Lee, Y. J, Kan, S, Nojima, T, Hoh, C, Nagatsuka, H, Nagai, N

Bioceramics 1999年

口腔粘膜の扁平上皮癌の癌化過程におけるEpidermal growth factor receptor の免疫組織化学的検討

永井教之, 劉桂需, 長塚仁, 石割裕三, 井上正久, 野島鉄人

岡山歯学会雑誌 1999年

46 : A case report of malignant melanoma in situ of the palate

Hibi K., Ishiwari Y., Nagaoka N., Inoue M., Nagatsuka H., Nagai N.

Oral medicine & pathology 3 ( 2 ) 114 - 114 1998年12月

The Effect of BMP on the Monocyte-Macrophage-Mesenchymal Cell Interaction in Ectopic Bone Formation

Shim Hong In, Kim Shin Yoon, Ryoo Hyun Mo, Yuzo ISHIWARI, Hitoshi NAGATSUKA, Song HAN, Noriyuki NAGAI, Department of Oral Pathology Kyungpook National University, Department of Orthopedics Kyungpook National University, Department of Oral Biochemistry Kyungpook National University, Department of Oral Pathology Okayama University, Department of Oral Pathology Okayama University, Department of Oral Pathology Okayama University, Department of Oral Pathology Okayama University

Journal of hard tissue biology 7 ( 1 ) 11 - 15 1998年6月

記述言語：英語出版者・発行元：日本硬組織研究技術学会

Partially purified bovine bone morphogenetic protein (BMP) absorbed in fibrous glass membrane (FGM) was implanted to the dorsal subcutaneous tissue of rats to investigate the effect of BMP on the monocytes/macrophages in ectopic bone formation. FGM alone was implanted as a control. The implants were harvested at 1, 2, and 3 weeks after implantation, then prepared for routine light microscopic and electron microscopic observations. At 1 week with some formations of multinucleated giant cells, many macrophages showing a positive immunoreaction for ED1 Ab, as well as mesenchymal cells, migrated into the BMP-FGM complex. The recruited intact macrophages in the BMP-FGM complex revealed more abundant cytoplasmic organelles and aclose intercytcplasmic relationship with the intermingling proliferated mesenchymal cells. However macrophages in the control showed less cellular activity due to the toxicity of FGM. These findings support the suggestion that BMP may serve as a potent chemoattractant to recruit monocytes as well as mesenchymal stem cells, and their close relationship may act synergistically to promote the bone inductive cascade.

4. Gene expression of amelogenin in odontogenic tumors :

その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=357228

Nakano Keisuke, Nagatsuka Hitoshi, Inoue Masahisa, Takagi Tohru, Nagai Noriyuki

日本硬組織研究技術学会雑誌 = Journal of hard tissue biology 7 ( 2 ) 62 - 62 1998年

記述言語：英語出版者・発行元：日本硬組織研究技術学会

The distribtution of amelogenin mRNA was investigated in ameloblastoma and adenomatoid odontogeznic tumor (AOT). Digoxygenin(DIG) labeled RNA probe was prepared from human plexiform type amelobastoma. Sections of rat incisors human ameloblastoma and ACT were hybridized with the above-mentioned probe, and immuno-detection of the hybridized probe was performed with the DIG Ntucleic Acid Detection Kit. In the rat incisor, amelogenin mRNA was detected in the preameloblast and ameloblast. Amelogeuuin mRNA was detected mainly in the high columutar cells of the amelobastoma. Amelogenin mRNA was also detected in many duct-like and solid strtuctture tumor cells of AOT.

27D18 : Tumor of mandibular bone

その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306625

Tsujigiwa H., Nagatsuka H., Inoue M., Takagi H., Nagai N.

Oral medicine & pathology 2 ( 2 ) 109 - 109 1997年12月

Aging and ectopic bone formation induced by partially purified bone morphogenetic protein

Chun-Lin Qin, Murata Masaru, Nagatsuka Hitoshi, Inoue Masahisa, Nagai Noriyuki

歯科基礎医学会雑誌 39 ( 5 ) 572 - 582 1997年10月

Gene Expression of Chondro-Osseous Cells in Heterotopic Bone formation Induced by rhBMP-2

Hitoshi NAGATSUKA, Masahisa INOUE, Takumi AKAGI, Yuzo ISHIWARI, Liu GUIRU, Bingzhen HUANG, Tohru TAKAGI, Mohamed Gomaa Z0UAIR-ATTIA, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Biochemistry School of Dentistry Tokyo Medical and Dental University, Department of Oral Pathology School of Dentistry Okayama University:Department of Oral Pathology Faculty of Dental Medicine Al-Azahar University, Department of Oral Pathology School of Dentistry Okayama University

Journal of hard tissue biology 6 ( 1 ) 10 - 15 1997年6月

記述言語：英語

The in situ hybridization method was applied to observe the heterofopic cartilage-like tissue induced by recombinant human bone morphogenetic protein-2(rhBMP-2). The localization of collagen II protein, osteopontin (OPN) and osteocalcin (OCN) mRNAs was evaluated in rhBMP-2 induced tissue in comparision with the cartilage tissue in normal endochondral ossification mode of the rat fetus. In the heterotopic cartilage-like tissue, OPN and OCN mRNA were detected in chondrocyte-like cells; collagen II was noted in the cartilago-like matrix. In the cartilage tissue in the endochondral ossification of the fetal rat tibia, OCN mRNA was absent in chondrocytes. These results suggest that the chondrocyte-like cells in the BMP induced cartilage-like tissue have the function of osteoblasts. The chondrocyte-like cells in the inductive cartilage-like tissue may be designated as chondro-osseous cells instead of chondrocytes. The heterotopic chondro-osseous tissue formation induced by rhBMP-2 differs from normal endochondral ossification in cell differentiation and function.

30D19: Left buccal tumor (Proceedings of the 7th Annual Meeting of The Japanese Society for Oral Pathology)

その他リンク： http://search.jamas.or.jp/link/ui/1998047081

Murata M., Inoue M., Nagatsuka H., Nagai N.

Oral medicine & pathology 2 ( 1 ) 52 - 52 1997年6月

Histopathological and Immunohistochemical Study of Heterotopic Chondro-Osseous Tissue Formation Induced by S-200 BMP

Masahisa INOUE, Chun-Lin QIN, Tetundo NOJIMA, Hitoshi NAGATSUKA, Masaru MURATA, Yukio NOSAKA, Takumi AKAGI, Katsuhiro KURODA, Masaru MABUCHI, Keifuku HOH, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University

Journal of hard tissue biology 5 ( 1 ) 1 - 6 1996年3月

記述言語：英語

The composite of S-200 bone morphogenetic protein (S-200 BMP) and fibrous glass membrane (FGM) was implanted in the dorsal subcutaneous tissues of rats, and the tissues were examined histopathologically and immunohistochemically at 1, 2 and 3 weeks after implantation. At 1 week after implantation, the explants showed a three zone structure which included the inner zone mainly consisting of FGM, the intermediate zone of chondro-osseous tissue containing collagen type II in the matrix, and the peripheral zone of connective tissue containing collagen type I. At. 2 weeks, osteocalcin was detected in the hypertrophic chondroblast-like cells in the chondro-osseous tissue, a phenomenon which does not exist in the normal endochondral ossification mode of long bones. At 2 weeks, collagen type I positive eosinophilic chondro-osseous tissue without collagen II was present between the basophilic chondro-osseous tissue containing collagen II, osteocalcin and the woven bone mingled with connective tissue at the periphery. At 3 weeks, bone morrow was formed and collagen type II still remained in the chondro-osseous tissue. Our findings indicate that S-200 BMP/FGM induces the chondro-osseous tissue through a process like that in the endochondral ossification mode but the matrix components and cell differentiation patterns dirrer from those in the nornrai fetal endochondral ossification process.

分子量早期限定部分精製BMPの性状と骨I型コラーゲンキャリア

永井教之, 村田勝, 辻極秀次, 井上正久, 長塚仁, 中野敬介, 赤木巧, 衣田圭宏, 完山学, 秦春林, 張紹全, 此内浩信, 韓松, 黄炳珍, 譚軍, 劉桂需, Gomah Atia, 日比一光

Journal of hard tissue biology = 日本硬組織研究技術学会雑誌 5 ( 2 ) 163 - 173 1996年

記述言語：日本語出版者・発行元：日本硬組織研究技術学会

A modofied method was established to extracted BMP from bovine bone. SDS-PAGE and Westen blotting were apllied to analize the components of this partially purified BMP. In addition the amino acid sequence was studied using protein sequencer to analyze the unknown bands. Type I collagen derived from bone was used as a carrier whose properties and advantages were studied. The following conclusions are made from this study. 1. The limitation of molecluar weight in tbe early step of BMP purification procedure improves purification efficiency and stability, which makes the applicadon of BMP in big animalos possible. 2. In this method the purified BMP product also contains histone H3, histone 2B and other unknown proteins as well as BMP-2. 3. The atelocollagen derived from bone has proved to be an useful carrier for BMP. This carrier shows characteristics of self absorption and recalcification when embedded in tissues. 4. The minimun bone induction dose of BMP at each step (G-Ext:500μg, Hep:30μg, S200:50μg) and the optimal bone induction dose(G-Ext:5.0mg, Hep:2.0mg, S200:0.3mg) are determined, which makes the applicalion of BMP in cellular biology possible.