Faculty Profiles - NAGATSUKA Hitoshi

Papers - NAGATSUKA Hitoshi

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Immunohistochemical Evaluation of Type IV Collagen Alpha Chains in Oral Malignant Melanoma(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)

Rivera Rosario Santos, Nagatsuka Hitoshi, Lee You-Jin, Tamamura Ryo, Katase Naoki, Liu Yue-Heng, Nagai Noriyuki

Journal of hard tissue biology 14 ( 2 ) 234 - 234 2005

Vasculogenic Mimicry of Oral Malignant Melanoma(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)

Lee You-jin, Rivera Rosario Santos, Nagatsuka Hitoshi, Siar Chong-Huat, Han Hg Kok, Yamagata Eiji, Nagai Noriyuki

Journal of hard tissue biology 14 ( 2 ) 300 - 301 2005

Stable long-term induction of perforin-positive CD8+ T cells in gut by oral administration of streptococcal preparation OK-432. International journal

Norifumi Mino, Yoshio Naomoto, Yasuhiro Shirakawa, Junji Matsuoka, Tetsuji Nobuhisa, Mehmet Gunduz, Kazuhiro Noma, Hitoshi Nagatsuka, Masahiko Kobayashi, Tomoki Yamatsuji, Minoru Haisa, Akira Gochi, Noriaki Tanaka

International journal of molecular medicine 14 ( 5 ) 801 - 806 2004.11

Language：English Publishing type：Research paper (scientific journal) Publisher：PROFESSOR D A SPANDIDOS

Perforin is known as a pore-forming cytotoxic granule released from cytotoxic T cells. Previous experiments in vitro revealed the presence of precursor cells that are capable of producing perforin in the immune system cells. The present study was undertaken to examine whether perforin-positive cells could be induced in the digestive tract and to characterize their precursor cells. Expression of perforin-positive cells in the intestine of Balb/c mice induced by OK-432 was analyzed by immunohistochemical staining and RT-PCR. Oral treatment of Balb/c mice with OK-432 resulted in the occurrence of perforin-positive cells in the inferior segment of small intestine, the superior segment of large intestine, mesenteric lymph nodes and spleen. In the small intestine, perforin-positive cells were found in the lamina propria mucosa. The presence of perforin-positive cells was also noted following long-term OK-432 treatment. Similar results were obtained following treatment with biological response modifiers such as lipopolysaccharide. In mice with GVHD (graft-versus-host disease), the presence of perforin-positive cells was noted in the small intestine and spleen. When the serial sections of the small intestinal mucosa from OK-432-treated mice were immunostained with anti-perforin, anti-CD8 and anti-asialo-GM1 antibodies, the perforin-positive cells were found to be CD8-positive. The results suggest that CD8(+) cells in lamina propria mucosa play a significant role as effectors in the mucosal immune system which is activated by various stimuli.

DOI： 10.3892/ijmm.14.5.801

Localization of heparanase in esophageal cancer cells: Respective roles in prognosis and differentiation International journal

Takaomi Ohkawa, Yoshio Naomoto, Munenori Takaoka, Tetsuji Nobuhisa, Kazuhiro Noma, Takayuki Motoki, Toshihiro Murata, Hirokazu Uetsuka, Masahiko Kobayashi, Yasuhiro Shirakawa, Tomoki Yamatsuji, Nagahide Matsubara, Junji Matsuoka, Minoru Haisa, Mehmet Gunduz, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Masao Hosokawa, Motowo Nakajima, Noriaki Tanaka

Laboratory Investigation 84 ( 10 ) 1289 - 1304 2004.10

Language：English Publishing type：Research paper (scientific journal) Publisher：NATURE PUBLISHING GROUP

In this study, we examined the distribution of heparanase protein in 75 esophageal squamous cell carcinomas by immunohistochemistry and analyzed the relationship between heparanase expression and clinicopathological characteristics. In situ hybridization showed that the mRNA expression pattern of heparanase was similar to that of the protein, suggesting that increased expression of the heparanase protein at the invasive front was caused by an increase of heparanase mRNA in tumor cells. Heparanase expression correlated significantly with depth of tumor invasion, lymph node metastasis, tumor node metastasis (TNM) stage and lymphatic invasion. Overexpression of heparanase in esophageal cancers was also associated with poor survival. In addition to its localization in the cytoplasm and cell membrane, heparanase was also identified in the nuclei of normal epithelial and tumor cells by immunohistochemistry. Furthermore, nuclear heparanase was detected in nuclear extract of cancer cell lines by Western blot and immunohistochemistry. Examination of the role of nuclear heparanase in cell proliferation and differentiation by double immunostaining for proliferating cell nuclear antigen (PCNA) and cytokeratin 10 (CK10) showed significant relationship between nuclear heparanase expression and differentiation (heparanase vs CK10), but not for proliferative state of esophageal cancer cells (heparanase vs PCNA). Our results suggest that cytoplasmic heparanase appears to be a useful prognostic marker in patients with esophageal cancer and that nuclear heparanase protein may play a role in differentiation. Inhibition of heparanase activity may be effective in the control of esophageal tumor invasion and metastasis.

DOI： 10.1038/labinvest.3700159

In vitro response of osteoblast-like and odontoblast-like cells to unsubstituted and substituted apatites International journal

Miho Inoue, Racquel Z. LeGeros, Masahisa Inoue, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Toshio Yamamoto, Noriyuki Nagai

Journal of Biomedical Materials Research - Part A 70 ( 4 ) 585 - 593 2004.9

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

Different types of calcium phosphate compounds [calcium-deficient apatite (CDA); beta-tricalcium phosphate (β-TCP); biphasic calcium phosphate (BCP)] are commercially available for medical and dental applications as bone substitute materials. Most of the reported in vitro studies on cell-material interactions have used osteoblast-like cells. The purpose of this study was to investigate the in vitro response of osteoblast-like (MC3T3-E1) and odontoblast-like (MDPC23) cells on unsubstituted (HA) and substituted (F-substituted) apatites. MC3T3-E1 and MDPC23 were cultured in alpha-modified medium containing 10% fetal bovine serum, ascorbic acid (50 μg/mL) and β-glycerophosphate (2 mM). The cells were seeded on pellets made from HA, and FAp (with low, medium, and high F concentrations). Cell morphology was observed after 7 and 14 days using scanning electron microscopy (SEM). Cell attachment and differentiation were determined from the DNA content, alkaline phosphatase (ALP) activity, and total collagen content. Pellet surface composition was characterized by using Fourier Transform infrared spectroscopy. MC3T3-E1 and MDPC23 cells on HA were normal in shape and in fusion but not on FAp. Results of this study showed that the pattern of cell proliferation of osteoblast-like cells was different from that of the odontoblast-like cells. This study suggests that cell morphology, fusion, and proliferation on biomaterial surfaces depend on cell type (osteoblast-like vs odontoblast-like cell) and biomaterial composition (unsubstituted vs substituted F-apatites). © 2004 Wiley Periodicals, Inc.

DOI： 10.1002/jbm.a.30116

In vitro response of osteoblast-like and odontoblast-like cells to unsubstituted and substituted apatites. International journal

Miho Inoue, Racquel Z LeGeros, Masahisa Inoue, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Toshio Yamamoto, Noriyuki Nagai

Journal of biomedical materials research. Part A 70 ( 4 ) 585 - 93 2004.9

Language：English Publishing type：Research paper (scientific journal)

Different types of calcium phosphate compounds [calcium-deficient apatite (CDA); beta-tricalcium phosphate (beta-TCP); biphasic calcium phosphate (BCP)] are commercially available for medical and dental applications as bone substitute materials. Most of the reported in vitro studies on cell-material interactions have used osteoblast-like cells. The purpose of this study was to investigate the in vitro response of osteoblast-like (MC3T3-E1) and odontoblast-like (MDPC23) cells on unsubstituted (HA) and substituted (F-substituted) apatites. MC3T3-E1 and MDPC23 were cultured in alpha-modified medium containing 10% fetal bovine serum, ascorbic acid (50 microg/mL) and beta-glycerophosphate (2 mM). The cells were seeded on pellets made from HA, and FAp (with low, medium, and high F concentrations). Cell morphology was observed after 7 and 14 days using scanning electron microscopy (SEM). Cell attachment and differentiation were determined from the DNA content, alkaline phosphatase (ALP) activity, and total collagen content. Pellet surface composition was characterized by using Fourier Transform infrared spectroscopy. MC3T3-E1 and MDPC23 cells on HA were normal in shape and in fusion but not on FAp. Results of this study showed that the pattern of cell proliferation of osteoblast-like cells was different from that of the odontoblast-like cells. This study suggests that cell morphology, fusion, and proliferation on biomaterial surfaces depend on cell type (osteoblast-like vs odontoblast-like cell) and biomaterial composition (unsubstituted vs substituted F-apatites).

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口腔扁平上皮癌における癌抑制遺伝子候補としてのCaspase-6の検討

神農泰生, 長塚仁, Gunduz Esra, Gunduz Mehmet, 辻極秀次, 玉村亮, 清水憲二, 永井教之

Journal of Oral Biosciences 46 ( 5 ) 422 - 422 2004.9

Influence of CpG island methylation status in O⁶-metnylguanine- DNA methyltransferase expression of oral cancer cell lines International journal

Jun Murakami, Jun Ichi Asaumi, Yuu Maki, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Susumu Kokeguchi, Tetsuyoshi Inoue, Shoji Kawasaki, Noriaki Tanaka, Donald MacPhee, Nagahide Matsubara, Kanji Kishi

Oncology Reports 12 ( 2 ) 339 - 345 2004.8

Language：English Publishing type：Research paper (scientific journal) Publisher：PROFESSOR D A SPANDIDOS

It is known that the O6-methylguanine-DNA methyltransferase (MGMT) gene is susceptible to epigenetic regulation associated with an altered frequency of CpG methylation. To investigate whether epigenetic regulation of the MGMT gene might lead to significant reductions in the expression levels of cancer cells, we sought evidence of a link between the methylation status of the MGMT promoter and the expression levels of seven human oral cancer cell lines. We found two frequently methylated regions: the 5′ region extending from nt 690 to nt 893 in the promoter, and the more 3′ region extending from nt 1060 to nt 1151 in the untranslated first exon. The 3′ region was hypermethylated independently of MGMT expression levels in all cell lines. By contrast, in the three MGMT-downregulated cell lines (SAS, Hep2, HO-1-u-1), the levels of MGMT expression were inversely related to the density of 5′ region of the methylated CpGs in the MGMT promoter. Our results implied that the transcriptional inactivation of MGMT might require methylation of the 5' region, but not that of the 3′ region in oral cancer cell lines. We further explored the role of methylation in MGMT expression by treating cells with 5-Aza-2′-deoxycytidine (5Aza-dC). 5Aza-dC treatment led to the partial or complete cytosine demethylation of two frequently methylated MGMT regions in all cell lines. 5Aza-dC succeeded in upregulating of the MGMT mRNA levels in only 2 of 7 cell lines (HSC3 and HO-1-u-1), and in fact reduced MGMT mRNA in the other 5 cell lines. Furthermore, 5Aza-dC had an inhibitory effect on MGMT protein levels in all cell lines. Our results suggest that MGMT levels may not revert after 5Aza-dC treatment. Based on our findings, the regulation of MGMT expression appears to be more complex than previously thought, although it is at least partially influenced by CpG methylation. Accordingly, care should be taken interpreting the link between MGMT methylation and expression.

Differential Expression of Collagen IV alpha 1 to alpha6 Chains in Basement Membranes of Malignant Odontogenic Tumors

Hidetaka Fujii, Hitoshi Nagatsuka, You Jin Lee, Taisei Shinnou, Ryo Tamamura, Jing Xiao, Noriyuki Nagai, Ichiro Naitou, Giichi Sado, Toshihiro Nakagawa

Journal of Hard Tissue Biology 13 ( 3 ) 111 - 115 2004.1

Publishing type：Research paper (scientific journal)

Type IV collagen, the major component of basement membrane (BM), demonstrates a stage- and position-specific distribution of its isoforms during tooth development. To determine its localization in BM of malignant odontogenic neoplasms, immunohistochemistry using six anti-alpha(IV) chain-specific monoclonal antibodies was performed. Results disclosed that BM demonstrated an irregular alpha(IV) chain profile in tooth germ as compared to benign odontogenic neoplasms. No alpha3(IV) chains were detected. Expression of alphal(IV)/ alpha2(IV) and alpha4(IV), alpha5(IV)/alpha6(IV) chains was ameloblastic fibro-odontosarcoma, but not the inductive hard tissues. Ameloblastic carcinoma showed specific alphal(IV)/alpha2(IV) chain loss, while primary intraosseous carcinoma demonstrated a discontinuous alphal (I V)/alpha2(TV) and alpha5(IV)/alpha6(IV) staining pattern. The present results suggest that modification and remodeling of BM collagen IV alpha chains occur during malignant odontogenic neoplasms’ progression. © 2004, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.13.111

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Differential Expression of Type IV Collagen alpha 1 to alpha 6 Chains in Basement Membrane of Human Tooth Germ and Odontogenic Tumors

Hidetaka Fujii, Hitoshi Nagatsuka, You Jin Lee, Taisei Shinnou, Ryo Tamamura, Jing Xiao, Noriyuki Nagai, Ichiro Naitou, Giichi Sado, Toshihiro Nakagawa

Journal of Hard Tissue Biology 13 ( 3 ) 103 - 109 2004.1

Publishing type：Research paper (scientific journal)

Type IV collagen, the major component of basement membrane (BM), demonstrates a stage- and position-specific distribution of its isoforms during tooth development. To determine its localization in BM of benign odontogenic neoplasms, immunohistochemistry using six anti-alpha(IV) chain-specific monoclonal antibodies was performed. Results disclosed that BM demonstrated an irregular alpha(IV) chain profile in tooth germ as compared to benign odontogenic neoplasms. No alpha3(IV) chains were detected. Expression of alphal(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) chains was stronger in desmoplastic than in ordinary ameloblastomas. The adenomatoid odontogenic tumor distinctly expressed these chains in BM of cribriform areas and hyaline materials (which was also alpha4(IV)-positive), but weakly around epithelial whorls/rosettes/ nests and mineralized foci. These five chains also stained BM and tumor cells of ameloblastic fibroma and odontoma but not the inductive hard tissues. The present results suggest that modification and remodeling of BM collagen IV alpha chains occur during odontogenic neoplasms progression. © 2004, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved. © 2004, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.13.103

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Cell Differentiation of MDPC-23 Cells Derived from Mouse Papilla Tissues Reviewed

Kazuo Ichikawa, Hitoshi Nagatsuka, Hidetsugu Tsujigiwa, Noriyuki Nagai

Journal of Hard Tissue Biology 13 ( 3 ) 131 - 135 2004.1

Language：English Publishing type：Research paper (scientific journal)

For the purpose of developing a hybrid type dentin with periodontal membrane implant, we conducted in vitro and in vivo experiments using a mouse dental papilla-derived cell line MDPC-23 to study anatomical and functional differentiation. Our results show that MDPC-23 cells possess dentin-inducing characteristics and differentiate into odontoblast-like cells extending cytoplasmic projection unilaterally. © 2004, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.13.131

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Distribution of Collagen Type IV α1-6 Chains and MMP in Oral Squamous Cell Carcinoma Reviewed

Ryo Tamamura, Hitoshi Nagatsuka, You Jin Lee, Jing Xiao, Noriyuki Nagai, Ichiro Naito, Yoshikazu Sado, Toshiyuki Kawakami

Journal of Hard Tissue Biology 13 ( 3 ) 125 - 130 2004.1

Language：English Publishing type：Research paper (scientific journal)

Destruction of basement membrane is an important element in invasion of cancer cells. Type IV collagen, the major component of basement membrane has six distinct α chains. Matrix metalloproteinases(MMPs) are enzymes that can resolve extracellular matrix. In this study, we investigated the localization of six α chains and MMPs in oral squamous cell carcinoma immunohistochemically. In well differentiated squamous cell carcinoma, the localization of α chains showed various patterns. α1 (IV),α2 (IV), α5 (IV) and α6 (IV) chains were stained in almost all basement membranes in the central of cancer. Unique staining with only α5 (IV) and α6 (IV) chains was also observed. In the invasive point of cancer, all a chains mainly became negative, but MMP-2 and MMP-9 were stained strongly. In poorly differentiated squamous cell carcinoma, both a chains and MMPs were not stained. Furthermore, we examined the correlation between distribution of α1 chain and invasive pattern of cancer. We found that the disappearence of α chains correlated closely with invasive activity of cancer. © 2004, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.13.125

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Original: Influence of Three-Dimensional Scaffold on Bone Induction by KUSA/A1 Cells

Andrea Paola Rodriguez, Hidetsugu Tsujigiwa, Silvia Borkosky, Phuu Pwint Han, Ryo Tamamura, Mehmet Gunduz, Hitoshi Nagatsuka, Noriyuki Nagai, Liliana Missana

Journal of Hard Tissue Biology 13 ( 2 ) 91 - 96 2004.1

Language：English Publishing type：Research paper (scientific journal)

Recently, atelocollagen has been used in tissue engineering as one of the most useful scaffolds for bone formation. However, the optimal scaffold-structure for bone engineering has not been determined. Hence, we compared the honeycomb porous structure (HPS) and the interconnected porous structure (IPS) with or without KUSA/A1 cells in vivo. KUSA/A1 cells alone, HPS alone, IPS alone, KUSA/A1-HPS and KUSA/A1-1PS were implanted in the subcutaneous pockets of 4-weeks-old male SCID mice. The implants were examined radiologically and histologically 1, 2,4 and 8 weeks after implantation. Radiologically, small radiopaque islands of new bone were observed in the implant of KUSA/A1 cells alone. No radiopacity was observed with both scaffols alone. In the KUSA/A 1 -HPS implant, the scaffold was partially filled with radiopaque areas. In contrast, in the KUSA/A1-IPS implant, the whole scaffold was filled with a well-defined radiopaque area. Histologically, KUSA/A1 cells alone induced only small nests of new bone. HPS alone produced inflammatory reaction. A large bone was observed in implants of KUSA/A1 cells combined with both scaffolds, but the scaffold was completely filled with new bone without inflammatory reaction within a shorter period in the KUSA/A1-IPS compared to KUSA/A1-HPS implant. Our results indicated that a cotton structured scaffold plays an important role in carrying the cells, providing the precise size, shape and comfortable environment. These results have potential impact to enhance existing therapeutic strategies. © 2004, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.13.91

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Radiographic examination of dentigerous cysts in the transitional dentition

Y. Shibata, J. Asaumi, Y. Yanagi, N. Kawai, M. Hisatomi, H. Matsuzaki, H. Konouchi, H. Nagatsuka, K. Kishi

Dentomaxillofacial Radiology 33 ( 1 ) 17 - 20 2004.1

Language：English Publishing type：Research paper (scientific journal) Publisher：BRITISH INST RADIOLOGY

Objectives: To examine radiographically the relationship between the deciduous tooth and dentigerous cyst of the permanent successor during the transitional dentition. Methods: From a retrospective review of all patients who visited our institution from April 1988 to August 2001, 70 patients under 16 years of age who had histologically confirmed dentigerous cysts that had developed from the central incisor to the second premolar were identified. These 70 patients were investigated using panoramic and periapical radiographs. Results: In most cases (54 cases; 77.1%) the cyst was in the premolar region. Of the 54 premolars with dentigerous cysts, the overlying deciduous tooth had already been lost in 7 cases. Of the 47 remaining premolars with associated deciduous tooth, 35 (74.5%) had bone resorption of the periapical or bifurcation region, or irregular resorption of the associated deciduous tooth. Of the remaining 12 deciduous teeth with no periapical lesions, 9 had been treated with root canal therapy. Thus, 44 of these 47 cases (93.6%) had the possibility of inflammation at the deciduous tooth associated with the dentigerous cyst. Evidence from one case in the present study suggesting the process by which cyst development occurs is also given. Conclusion: Inflammatory change at the apex of the deciduous tooth may bring on a dentigerous cyst of the permanent successor. © 2004 The British Institute of Radiology.

DOI： 10.1259/dmfr/24148363

Original: Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice

Hitoshi Nagatsuka, Yoshiko Kitamura, Hidetsugu Tsujigiwa, Lee You-jin, Mehmet Gunduz, B. Z. Huang, Noriyuki Nagai, Chong Huat Siar, Toshifumi Komori, Mathieu Lefevre

Journal of Hard Tissue Biology 13 ( 1 ) 35 - 43 2004.1

Language：English Publishing type：Research paper (scientific journal)

Odontogenesis and osteogenesis are two distinct yet closely related processes that share common signalling pathways including regulatory control by Cbfal. Although Cbfal is well-recognized as the master regulator of osteoblastic differentiation, little is known of its precise role during odontogenesis. We sought to clarify this by examining morphogenesis of teeth and matrix protein gene expression in cbfal-knock-out mice at ed 17.5 and day 0 of birth, and comparing it with presumptive mandible bone-forming site. Results demonstrated morphological and functional differentiation differences between these two processes: incisor tooth germ showed morphological and functional differentiation of odontoblasts with expression of osteopontin and osteocalcin whereas the presumptive mandible bone-forming site showed lack of osteoblastic differentiation, and no expression of these two matrix proteins. Stage-specific and cytodifferentiation differences were also observed between incisor and molar tooth germs: incisor tooth germ progressed to the bell stage with odontoblast and ameloblast differentiation, and expression of tooth-specific proteins, amelogenin and dentine sialophosphoprotein, whereas molar tooth germ showed maturational arrest at bud to cap stage. Present findings suggest that 1. The transcription factor Cbfa-1 is associated with morphogenesis of teeth and matrix protein gene expression. 2. Compared to the incisor tooth germ, the molar tooth germ is more strongly subjected to control by Cbfal, and 3. In Cbfal-knockout mice, the odontoblast-like cells in the incisor and the spindle cells in the presumptive mandible forming region showed different patterns of gene expression of matrix proteins common to teeth and bones. © 2004, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.13.35

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Osteoblastoma of the Mandible: Report of A Case with Immunohistochemical Analysis

Takashi Fujii, Takaaki Ueno, Miwa Kanou, Nobuhisa Ishida, Toshimasa Kagawa, Hitoshi Nagatsuka, Noriyuki Nagai, Toshio Sugahara

Asian Journal of Oral and Maxillofacial Surgery 16 ( 1 ) 59 - 62 2004

A columella formation in the middle ear by BMP-2/collagen composites: An experimental study using tissue engineering Reviewed

Takeda Y, Tsujigiwa H, Nishizaki K, Yoshino T, Orita Y, Nagatsuka H, Nagai N

PROCEEDINGS OF THE 3RD SYMPOSIUM ON MIDDLE EAR MECHANICS IN RESEARCH AND OTOLOGY 116 - 120 2004

Experimental mastoid obliteration by BMP-2/collagen composites Reviewed

Nishizaki K, Tsujigiwa H, Takeda Y, Yoshino T, Maeta M, Fukushima K, Nagatsuka H, Nagai N

PROCEEDINGS OF THE 3RD SYMPOSIUM ON MIDDLE EAR MECHANICS IN RESEARCH AND OTOLOGY 121 - 125 2004

Expression of Notch in a case of osteosarcoma of the maxilla

Toshiyuki Kawakami, C. H. Siar, K. H. Ng, T. Shimizu, N. Okafuji, S. Kurihara, H. Hasegawa, H. Tsujigiwa, H. Nagatsuka, N. Nagai

European Journal of Medical Research 9 ( 11 ) 533 - 535 2004

Language：English Publishing type：Research paper (scientific journal) Publisher：I HOLZAPFEL VERLAG GMBH

In this immunohistochemical examination, the expression of Notch1 peptide was detected in neo-plastic cells in a case of osteosarcoma of the maxilla of a 31 -year-old Indonesian male patient. Notch1 peptide appeared in the cytoplasm of neoplastic cells of comparatively well-differentiated areas of the osteosarcoma, an osteoblastic area containing osteoid and/or immature bone tissues. The results suggest that Notch1 is closely related to cytological differentiation of acquisition of cytological characteristics in neoplastic cells of osteosarcoma. © I. Holzapfel Publishers 2004.

Inhibition of inducible NF-kappaB activity reduces chemoresistance to 5-fluorouracil in human stomach cancer cell line. Reviewed

Uetsuka H, Haisa M, Kimura M, Gunduz M, Kaneda Y, Ohkawa T, Takaoka M, Murata T, Nobuhisa T, Yamatsuji T, Matsuoka J, Tanaka N, Naomoto Y

Experimental cell research 289 ( 1 ) 27 - 35 2003.9

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