Faculty Profiles - NAGATSUKA Hitoshi

Papers - NAGATSUKA Hitoshi

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Nestin is a wide-spectrum abluminal cell marker of salivary gland tumors International journal

Hiroyuki Yanai, Yasuharu Sato, Hitoshi Nagatsuka, Tadashi Yoshino

Pathology International 63 ( 10 ) 496 - 501 2013.10

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY

Nestin is an intermediate filament that was first identified in neural progenitor cells. It is expressed in various cell types in the nervous system as well as in other systems. In the present study, we investigated nestin expression in non-neoplastic salivary gland tissue and in salivary gland tumors. In non-neoplastic salivary glands, nestin expression was observed in only a few abluminal cells. In contrast, diffuse nestin staining was observed in the abluminal cells of pleomorphic adenoma (11 of 11 cases), basal cell adenoma (7 of 7 cases), and epithelial-myoepithelial carcinoma (2 of 2 cases). The stromal cells in basal cell adenoma also expressed nestin. In adenoid cystic carcinoma (6 of 7 cases) and polymorphous low-grade adenocarcinoma (3 of 3 cases), nestin positive cells were observed focally. Nestin was not detected in Warthin tumor (6 cases), classical acinic cell carcinoma (2 cases), mucoepidermoid carcinoma (5 cases), or salivary duct carcinoma (4 cases). Because the nestin expression pattern in each histological salivary gland tumor type is unique, nestin could be a very useful abluminal cell marker for the diagnosis of salivary gland tumors. © 2013 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.

DOI： 10.1111/pin.12103

口腔癌に対するBCG生菌療法による抗腫瘍、延命効果の検討

村上純, 大原直也, 中山真彰, 辻極秀次, 長塚仁, 此内浩信, 柳文修, 畦坪輝寿, 浅海淳一

Journal of Oral Biosciences Supplement 2013 222 - 222 2013.9

カラゲニン誘発局所炎症反応に対するデクスメデトミジンの抑制作用

助川信太郎, 長塚仁, 宮脇卓也

Journal of Oral Biosciences Supplement 2013 212 - 212 2013.9

角化嚢胞性歯原性腫瘍の再発に関する臨床的検討

助川信太郎, 高畑和路, 片瀬直樹, 管野貴浩, 万代とし子, 高橋由佳, 篠原丈裕, 長塚仁, 古木良彦

Hospital Dentistry & Oral-Maxillofacial Surgery 25 ( 1 ) 27 - 31 2013.6

Language：Japanese Publisher：日本病院歯科口腔外科協議会

角化嚢胞性歯原性腫瘍の再発に関する臨床的検討を行った。病理組織学的に歯原性角化嚢胞および角化嚢胞性歯原性腫瘍と診断され、病理標本上病変の性状を正確に評価でき、かつ加療後に6ヵ月以上の経過観察が行えた27例を対象とした。発生部位は上顎5例、下顎22例で、上顎は全て臼歯部であった。X線所見において、単房性透過像は23例、多房性透過像は4例であった。腫瘍径は平均39.9mmで、5cm来満のものが17例、5cm以上のものが10例であった。娘細胞は8例にみられ、19例には見られなかった。腫瘍に接する歯の処置は、根治的処理を行ったものが16例、保存的処置を行ったものが11例であった。5例で再発を認め、再発率は18.5%であった。腫瘍径が5cm以上の群で、有意に再発率が高かった。腫瘍に接する歯を根治的に処置した群で優位に再発率が低かった。

Diagnostic value of MRI for odontogenic tumours

M. Fujita, H. Matsuzaki, Y. Yanagi, M. Hara, N. Katase, M. Hisatomi, T. Unetsubo, H. Konouchi, H. Nagatsuka, J. I. Asaumi

Dentomaxillofacial Radiology 42 ( 5 ) 2013.5

Language：English Publishing type：Research paper (scientific journal) Publisher：BRITISH INST RADIOLOGY

Objectives: To evaluate the diagnostic value of MRI for odontogenic tumours. Materials and methods: 51 patients with odontogenic tumours were subjected to preoperative MRI examinations. For tumours with liquid components, i.e. ameloblastomas and keratocystic odontogenic tumours (KCOTs), the signal intensity (SI) uniformity of their cystic components (US) was calculated and then their US values were compared. For tumours with solid components that had been examined using dynamic contrast-enhanced MRI (DCEMRI), their CI max (maximum contrast index), Tmax (the time when CImax occurred), CIpeak (CImax×0.90), Tpeak (the time when CIpeak occurred) and CI300 (i.e. the CI observed at 300 s after contrast medium injection) values were determined from CI curves. We then classified the odontogenic tumours according to their DCE-MRI parameters. Results: Significant differences between the US values of the ameloblastomas and KCOT were observed on T1 weighted images, T2 weighted images and short TI inversion recovery images. Depending on their DCE-MRI parameters, we classified the odontogenic tumours into the following five types: Type A, CIpeak > 2.0 and Tpeak < 200 s; Type B, CIpeak < 2.0 and Tpeak < 200 s; Type C, CI 300 > 2.0 and Tmax < 600 s; Type D, CI300 > 2.0 and Tmax > 600 s; Type E, CI300 < 2.0 and Tmax > 600 s. Conclusion: Cystic component SI uniformity was found to be useful for differentiating between ameloblastomas and KCOT. However, the DCE-MRI parameters of odontogenic tumours, except for odontogenic fibromas and odontogenic myxomas, contributed little to their differential diagnosis. © 2013 The British Institute of Radiology.

DOI： 10.1259/dmfr.20120265

Knockdown of Dkk-3 decreases cancer cell migration and invasion independently of the Wnt pathways in oral squamous cell carcinoma‑derived cells. International journal

Naoki Katase, Mathieu Lefeuvre, Hidetsugu Tsujigiwa, Masae Fujii, Satoshi Ito, Ryo Tamamura, Rosario Rivera Buery, Mehmet Gunduz, Hitoshi Nagatsuka

Oncology reports 29 ( 4 ) 1349 - 55 2013.4

Language：English Publishing type：Research paper (scientific journal)

Oral squamous cell carcinoma (OSCC) is thought to arise as the result of cumulative genetic or epigenetic alterations in cancer-associated genes. We focused on the Dickkopf-3 (Dkk-3) gene as a candidate tumor suppressor in OSCC. Dkk-3 is a potential tumor suppressor, and its downregulation has been reported in various types of malignancies. However, our previous data demonstrated that the Dkk-3 protein was dominantly expressed in OSCC tissue, and its expression was correlated with a high incidence of metastasis and with poor prognosis. In order to explain this paradox, we performed functional analyses of the Dkk-3 gene in cancer cell lines. RT-PCR revealed that Dkk-3 mRNA expression was observed in OSCC-derived cell lines but not in gastrointestinal or colorectal adenocarcinoma‑derived cell lines. The siRNA for Dkk-3 was transfected into Dkk-3-expressing cells, and the changes in cell proliferation, invasion and migration were assessed. The knockdown of Dkk-3 mRNA by siRNA transfection did not affect cell proliferation, but it significantly decreased cell migration and invasion. To further investigate the precise mechanism that contributes to the potential oncogenic function of Dkk-3, the Wnt canonical pathway and non-canonical pathways were assessed. Western blotting demonstrated that the effect of Dkk-3 knockdown on cell migration or invasion was not caused by activation of the Wnt pathways. These data demonstrated that Dkk-3 expression in OSCC was different than that in adenocarcinomas. Dkk-3 may possess an oncogenic function that is independent of Wnt signaling.

DOI： 10.3892/or.2013.2251

The role of bone marrow-derived cells during the bone healing process in the GFP mouse bone marrow transplantation model International journal

Hidetsugu Tsujigiwa, Yasuhisa Hirata, Naoki Katase, Rosario Rivera Buery, Ryo Tamamura, Satoshi Ito, Shin Takagi, Seiji Iida, Hitoshi Nagatsuka

Calcified Tissue International 92 ( 3 ) 296 - 306 2013.3

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Bone healing is a complex and multistep process in which the origin of the cells participating in bone repair is still unknown. The involvement of bone marrow-derived cells in tissue repair has been the subject of recent studies. In the present study, bone marrow-derived cells in bone healing were traced using the GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) were transplanted into C57BL/6 J wild mice. After transplantation, bone injury was created using a 1.0-mm drill. Bone healing was histologically assessed at 3, 7, 14, and 28 postoperative days. Immunohistochemistry for GFP; double-fluorescent immunohistochemistry for GFP-F4/80, GFP-CD34, and GFP-osteocalcin; and double-staining for GFP and tartrate-resistant acid phosphatase were performed. Bone marrow transplantation successfully replaced the hematopoietic cells into GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts or osteocytes in the repair stage were GFP-negative, whereas osteoclasts in the repair and remodeling stages and hematopoietic cells were GFP-positive. The results indicated that bone marrow-derived cells might not differentiate into osteoblasts. The role of bone marrow-derived cells might be limited to adjustment of the microenvironment by differentiating into inflammatory cells, osteoclasts, or endothelial cells in immature blood vessels. © 2012 Springer Science+Business Media New York.

DOI： 10.1007/s00223-012-9685-3

Comparative analysis of basal lamina type IV collagen α chains, matrix metalloproteinases-2 and -9 expressions in oral dysplasia and invasive carcinoma. International journal

Ryo Tamamura, Hitoshi Nagatsuka, Chong Huat Siar, Naoki Katase, Ichiro Naito, Yoshikazu Sado, Noriyuki Nagai

Acta histochemica 115 ( 2 ) 113 - 9 2013.3

Language：English Publishing type：Research paper (scientific journal)

The aim of this study was to compare the expressions of basal lamina (BL) collagen IV α chains and matrix metalloproteinases (MMP)-2 and MMP-9 in oral dysplasia (OED) and invasive carcinoma. Ten cases each of OEDs, carcinomas-in situ and oral squamous cell carcinomas (OSCCs) were examined by immunohistochemistry. Another 5 cases, each of normal and hyperplastic oral mucosa, served as controls. Results showed that α1(IV)/α2(IV) and α5(IV)/α6(IV) chains were intact in BLs of control and OEDs. In BLs of carcinoma-in situ, α1(IV)/α2(IV) chains preceded α5(IV)/α6(IV) chains in showing incipient signs of disruption. OSCCs exhibited varying degrees of collagen α(IV) chain degradation. MMP-2 and MMP-9 were absent in controls and OED, but weakly detectable in carcinoma-in situ. In OSCC, these proteolytic enzymes were expressed in areas corresponding to collagen α(IV) chain loss. Enzymatic activity was enhanced in higher grade OSCC, and along the tumor advancing front. Overall the present findings suggest that loss of BL collagen α(IV) chains coincided with gain of expression for MMP-2 and MMP-9, and that these protein alterations are crucial events during progression from OED to OSCC.

DOI： 10.1016/j.acthis.2012.05.001

Conditions inhibiting eruption of permanent first molars

Hidenobu Matsuzaki, Yoshinobu Yanagi, Naoki Katase, Hitoshi Nagatsuka, Marina Hara, Masakazu Ashida, Teruhisa Unetsubo, Akiko Sato, Mariko Fujita, Toshihiko Takenobu, Jun Ichi Asaumi

Pediatric Dentistry 35 ( 1 ) 67 - 70 2013.1

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER ACAD PEDIATRIC DENTISTRY

Purpose: The purpose of this study was to evaluate the radiological and histopathological findings of 11 patients with unerupted first molars to verify the factors obstructing spontaneous eruption. Methods: The patients' clinical, radiological, and histopathological data were evaluated retrospectively to determine histopathological diagnosis, radiographic findings, methods of surgical management, and postoperative course. Results: This study involved 4 male and 7 female patients (mean age=9.5 years old). Nine cases involved the mandible. The patients' histopathological diagnoses included 3 odontogenic tumors, 2 odontogenic cysts, and 6 hyperplastic dental follicles. Radiographically, 10 cases showed characterless enlargement of the follicular space, while only 1 displayed radiopaque bodies. One patient with a tumor underwent enucleation, and 1 with a cyst underwent cystectomy and tooth extraction. The others underwent wide excision or partial excision of the surrounding tissue at the top of the impacted tooth. Tumor relapse was observed in 3 cases. Conclusion: Surgeons should perform aggressive treatment for patients with unerupted teeth because spontaneous eruption is rare in cases involving non-neoplastic lesions such as hyperplastic dental follicles.

Epithelium-poor type central odontogenic fibroma: An immunohistological study and review of the literature Reviewed

Takashi Kimura, Seigo Ohba, Hitoshi Yoshimura, Naoki Katase, Yoshiaki Imamura, Takaaki Ueno, Hitoshi Nagatsuka, Kazuo Sano

Journal of Hard Tissue Biology 22 ( 2 ) 273 - 278 2013

Language：English Publishing type：Research paper (scientific journal)

The object of this study was to determine the characteristics of central odontogenic fibroma (COF) in order to help achieve a correct diagnosis. Central odontogenic fibroma, which tends to be epithelium-poor, is an extremely rare type of odontogenic fibroma. The lack of data concerning COF, due to its low incidence, can make this entity difficult to diagnose. We herein report an epithelium-poor type of COF of the mandible, which we examined immunohistologically. We also provide a review of the previous English literature to expand the knowledge about this disease. Positive reactions for vimentin and negative reactions for desmin andα-smooth muscle actin (α-SMA) in COFs were common findings in the previous reports. We also noted similar immunohistological findings. Our data (vimentin positive,α-SMA and desmin negative) should contribute to correctly diagnosing epithelium-poor type COF. © 2013 The Hard Tissue Biology Network Association.

DOI： 10.2485/jhtb.22.273

Improvement of the efcacy of 5-aminolevulinic acid-mediated photodynamic treatment in human oral squamous cell carcinoma hsc-4

Masanao Yamamotoarf, Hirofumi Fujitaa, Naoki Katase, Keiji Inouec, Hitoshi Nagatsuka, Kozo Utsumi, Junzo Sasaki, Hideyo Ohuchi

Acta Medica Okayama 67 ( 3 ) 153 - 164 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：OKAYAMA UNIV MED SCHOOL

Ever since protoporphyrin IX (PpIX) was discovered to accumulate preferentially in cancer cells after 5-aminolevulinic acid (ALA) treatment, photodynamic treatment or therapy (PDT) has been developed as an exciting new treatment option for cancer patients. However, the level of PpIX accumulation in oral cancer is fairly low and insufficient for PDT. Ferrochelatase (FECH) and ATP-binding cassette transporter G2 (ABCG2) are known to regulate PpIX accumulation In addition, serum enhances PpIX export by ABCG2. We investigated here whether and how inhibitors of FECH and ABCG2 and their combination could improve PpIX accumulation and PDT efficacy in an oral cancer cell fine in serum-containing medium. ABCG2 inhibitor and the combination of ABCG2 and FECH inhibitors increased PpIX in the presence of fetal bovine serum (FBS) in an oral cancer cell fine. Analysis of ABCG2 gene silencing also revealed the involvement of ABCG2 in the regulation of PpIX accumulation. Inhibitors of FECH and ABCG2, and their combination increased the efficiency of ALA-PDT even in the pres¬ence of FBS. ALA-PDT-induced cell death was accompanied by apoptotic events and lipid peroxida-tion. These results suggest that accumulation of PpIX is determined by the activities of ABCG2 and FECH and that treatment with a combination of their inhibitors improves the efficacy of PDT for oral cancer, especially in the presence of serum © 2013 by Okayama University Medical School.

DOI： 10.18926/AMO/50408

The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands

Yao Wei Yuan, Ryo Tamamura, Lei Lei, Naoki Katase, Gulsan Ara Sathi, Satoshi Ito, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka

Journal of Hard Tissue Biology 22 ( 4 ) 433 - 438 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：JOURNAL HARD TISSUE BIOLOGY

Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy. © 2013 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved.

DOI： 10.2485/jhtb.22.433

Promotion of transplanted bone marrow-derived cell migration into the periodontal tissues due to orthodontic mechanical stress International journal

Mihoko Tomida, Hidetsugu Tsujigiwa, Keisuke Nakano, Rina Muraoka, Takami Nakamura, Norimasa Okafuji, Hitoshi Nagatsuka, Toshiyuki Kawakami

International Journal of Medical Sciences 10 ( 10 ) 1321 - 1326 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：IVYSPRING INT PUBL

Background: Bone marrow-derived cells (BMCs) have abilities of cell migration and differentiation into tissues/organs in the body and related with the differentiation of teeth or periodontal tissue including fibroblasts. Then, we examined the effect of orthodontic mechanical stress to the transplanted BMC migration into periodontal tissues using BMC transplantation model. Material and Method: BMC from green fluorescence protein (GFP) transgenic mice were transplanted into 8-week-old female C57BL/6 immunocompromised recipient mice, which had undergone 10 Gy of lethal whole-body-irradiation. Five mice as experimental group were received orthodontic mechanical stress using separator between first molar (M1) and second molar (M2) 1 time per week for 5 weeks and 5 mice as control group were not received mechanical stress. The maxilla with M1 and M2 was removed and was immunohistochemically analyzed using a Dako Envision + Kit-K4006 and a primary anti-GFP-polyclonal rabbit antibody. Immunohistochemically stained was defined as positive area and the pixel number of positive area in the periodontal tissue was compared with the previously calculated total pixel number of the periodontal tissue. Results: The immunohistochemistry revealed that GFP positive cells were detected in the periodontal tissues, both in the experimental and control specimens. The ratio of pixel number in the examination group showed 5.77 ± 3.24 % (mean ± SD); and that in the control group, 0.71±0.45 % (mean ± SD). The examination group was significantly greater than that of control group (Mann-Whitney U test: p<0.001). Conclusion: These results suggest that orthodontic mechanical stress accelerates transplanted BMC migration into periodontal tissues. © Ivyspring International Publisher.

DOI： 10.7150/ijms.6631

Osteogenic genes related to the canonic WNT pathway are down-regulated in ameloblastoma International journal

Gulsan A. Sathi, Hidetsugu Tsujigiwa, Satoshi Ito, Chong Huat Siar, Naoki Katase, Ryo Tamamura, Hidemitsu Harada, Hitoshi Nagatsuka

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 114 ( 6 ) 771 - 777 2012.12

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE INC

Objective: The aim of this study was to determine the expression of essential osteogenic genes related to the canonic WNT pathway, i.e., WDR5, sFRP-2, and their downstream genes, in ameloblastoma and to clarify their biologic impact on this neoplasm. Study Design: Forty-six paraffin-embedded ameloblastoma samples and ameloblastic (AM-1) and preosteoblastic (KUSA/A1) cell lines were used. Immunohistochemistry, Western blot, reverse-transcription polymerase chain reaction, and alkaline phosphatase (ALP) activity assay were performed. Results: WDR5, essential for osteoblast differentiation and canonic WNT pathway activation, was negative in most ameloblastoma cases and weakly expressed in AM-1 cells. Conversely, sFRP-2s was overexpressed. RUNX2 and C-MYC, downstream inductions of canonic WNT pathway activation, demonstrated weak mRNA expressions in ameloblastoma, suggesting WNT pathway impairment and WDR5 functional inactivity. Recombinant WDR5 weakly induced ALP activity of KUSA/A1 cells cultured in AM-1 conditioned medium. Conclusions: These findings suggest that WNT-related bone-forming genes are down-regulated in ameloblastoma. Concurrent sFRP-2 overexpression suggests that both bone-forming and bone-inhibiting genes equally contributed to reduced bone formation in this neoplasm. © 2012 Elsevier Inc.

DOI： 10.1016/j.oooo.2012.08.453

Transplanted Bone Marrow-Derived Cell Migration Into Periodontal Tissues Induced by Orthodontic Mechanical Stress Reviewed

Tomida M, Tsujigiwa H, Nakano K, Muraoka R, Nagatsuka H, Kawakami T

TRANSPLANTATION 94 ( 10 ) 555 2012.11

Diagnostic value of dynamic contrast-enhanced MRI for submucosal palatal tumors International journal

Hidenobu Matsuzaki, Yoshinobu Yanagi, Marina Hara, Naoki Katase, Miki Hisatomi, Teruhisa Unetsubo, Hironobu Konouchi, Toshihiko Takenobu, Hitoshi Nagatsuka, Jun Ichi Asaumi

European Journal of Radiology 81 ( 11 ) 3306 - 3312 2012.11

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER IRELAND LTD

Objectives: To evaluate the diagnostic value of dynamic contrast-enhanced MRI (DCE-MRI) for differentiating between benign and malignant tumors in the palate. Materials and methods: 26 patients with submucosal palatal tumors were preoperatively examined using DCE-MRI. Their maximum contrast index (CImax), time of CImax (Tmax), and washout ratios (WR300 and WR600) were determined from contrast index curves. The submucosal palatal tumors were divided into two groups according to their Tmax values: the early enhancement group (Tmax < 300 s) consisted of 9 malignant tumors and 6 benign tumors, while the late enhancement group (Tmax ≥ 300 s) included one malignant tumor and 10 benign tumors. We compared the following DCE-MRI parameters between the benign and malignant tumors: CImax and Tmax in all cases and CImax, Tmax, and the washout ratios in the early enhancement group. In addition, we performed a regression analysis of the relationships between tumor size and DCE-MRI parameters; i.e., CImax, Tmax, and washout ratios, among the malignant salivary gland tumors and pleomorphic adenomas. Results: In all cases and the early enhancement group, significant differences in Tmax were detected between the benign and malignant tumors (P < 0.001 and P < 0.05, respectively), and the optimal Tmax cutoff value for differentiating between them was found to be 165 s. None of the other parameters displayed significant differences between the benign and malignant tumors. Only the WR600 of the pleomorphic adenomas was significantly correlated with tumor size (R2 = 0.92, P < 0.001). Conclusions: Tmax is a useful parameter for distinguishing between benign and malignant submucosal palatal tumors. © 2012 Elsevier Ireland Ltd. All rights reserved.

DOI： 10.1016/j.ejrad.2012.04.009

Solid-type primary intraosseous squamous cell carcinoma of the mandible: A case report with histopathological and imaging features International journal

Hidenobu Matsuzaki, Naoki Katase, Tatsushi Matsumura, Marina Hara, Yoshinobu Yanagi, Hitoshi Nagatsuka, Seiji Iida, Jun Ichi Asaumi

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 114 ( 5 ) e71-7 - E77 2012.11

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE INC

Primary intraosseous squamous cell carcinoma (PIOSCC) is a rare malignant odontogenic tumor arising from odontogenic epithelial remnants within the jawbones. PIOSCC is histopathologically divided into 3 types: solid-type carcinoma, carcinoma derived from a keratocystic odontogenic tumor, and carcinoma derived from an odontogenic cyst. In this article, we report a case of solid-type PIOSCC involving reactive bone formation in the mandible in a 60-year-old female patient together with its histopathological and imaging findings. © 2012 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.oooo.2012.05.019

Ossifying fibroma of the maxilla: A case report including its imaging features and dynamic magnetic resonance imaging findings International journal

Marina Hara, Hidenobu Matsuzaki, Naoki Katase, Yoshinobu Yanagi, Teruhisa Unetsubo, Jun Ichi Asaumi, Hitoshi Nagatsuka

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 114 ( 4 ) e139-46 - E146 2012.10

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE INC

Ossifying fibroma (OF), a rare nonodontogenic tumor, is defined as a bone-related jawbone lesion. The main histopathological feature of OF is the replacement of bone by benign connective tissue. Ossifying fibroma usually occurs in the second to fourth decades of life and shows a predilection for females. Ossifying fibroma most commonly occurs in the mandible, and OF arising from the anterior part of the maxilla is rare. Ossifying fibromas display various radiographic findings, including varying degrees of radiolucency and radiopacity, depending on the proportions of their soft and hard tissue components. Depending on their components, it can be difficult to distinguish OF from other fibroosseous lesions and some odontogenic tumors by using conventional radiographs, computed tomography, and magnetic resonance imaging (MRI). We report a case of OF in the anterior maxilla in a 56-year-old man, together with its histopathological and imaging findings including the dynamic MRI findings. © 2012 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.oooo.2012.04.015

Heparanase and cyclooxygenase-2 gene and protein expressions during progression of oral epithelial dysplasia to carcinoma International journal

Hitoshi Nagatsuka, Chong Huat Siar, Hidetsugu Tsujigiwa, Yoshio Naomoto, Phuu Pwint Han, Mehmet Gunduz, Toshio Sugahara, Akira Sasaki, Motowo Nakajima

Annals of Diagnostic Pathology 16 ( 5 ) 354 - 361 2012.10

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE INC

Heparanase and cyclooxygenase-2 (COX-2) are 2 key enzymes that modulate diverse physiological processes during embryonic development and in adult life. Their deregulations have been implicated in the growth and progression of many cancer types. To date, comparatively little is known about the roles of these molecules during oral carcinogenesis. The aim of this study was to investigate the expression patterns of heparanase and COX-2 during progression of oral epithelial dysplasia (OED) to carcinoma. In situ hybridization and immunohistochemistry were performed on 5 cases of normal mucosa, 15 cases of OED, 5 cases of carcinoma in situ and/or microinvasive carcinoma, and 40 cases of oral squamous cell carcinoma (OSCC). Results demonstrated that heparanase and COX-2 messenger RNA and protein were absent in normal oral mucosa but were coexpressed in increasing intensity as OED progressed to OSCC. Concomitant heparanase- and COX-2-positive staining in the stromal cells suggests that OED/OSCC progression may be modulated by stromal-cancer cell interactions. Diffuse intense staining of poorly differentiated OSCC compared with staining localized to tumor nest periphery in well- and moderately differentiated OSCC suggests that heparanase and COX-2 overexpressions correlated with tumor grade. Strong expression of these enzymes in tumor cells at the advancing front suggests a role in local tumor spread. These results, taken together, suggest that heparanase and COX-2 might play complementary roles in the stepwise progression of OED to carcinoma. © 2012 Elsevier Inc.

DOI： 10.1016/j.anndiagpath.2012.02.004

Minor salivary gland tumors in the oral cavity: Diagnostic value of dynamic contrast-enhanced MRI International journal

Hidenobu Matsuzaki, Yoshinobu Yanagi, Marina Hara, Naoki Katase, Jun Ichi Asaumi, Miki Hisatomi, Teruhisa Unetsubo, Hironobu Konouchi, Toshihiko Takenobu, Hitoshi Nagatsuka

European Journal of Radiology 81 ( 10 ) 2684 - 2691 2012.10

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER IRELAND LTD

Objective: To evaluate the diagnostic value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for minor salivary gland tumors in the oral cavity. Materials and methods: Thirty-two patients with minor salivary gland tumors were examined preoperatively using DCE-MRI. Their maximum contrast index (CImax), time of CImax (Tmax), Tpeak; i.e., the time that corresponded to the CImax × 0.90, and washout ratios (WR300 and WR600) were determined from contrast index (CI) curves. We compared these parameters between benign and malignant tumors and among the different histopathological types of minor salivary gland tumors. Then, we categorized the patients' CI curves into four patterns (gradual increase, rapid increase with high washout ratio, rapid increase with low washout, and flat). Results: Statistically significant differences in Tmax (P = 0.004) and Tpeak (P = 0.002) were observed between the benign and malignant tumors. Regarding each histopathological tumor type, significant differences in Tmax (P < 0.001), Tpeak (P < 0.001), and WR600 (P = 0.026) were observed between the pleomorphic adenomas and mucoepidermoid carcinomas. It was difficult to distinguish between benign and malignant tumors using our CI curve classification because that two-thirds of the cases were classified into the same type (gradual increase). Conclusion: The DCE-MRI parameters of minor salivary gland tumors contributed little to their differential diagnosis compared with those for major salivary gland tumors. During the diagnosis of minor salivary gland tumors, Tmax is useful for distinguishing between benign and malignant tumors. © 2011 Elsevier Ireland Ltd.

DOI： 10.1016/j.ejrad.2011.11.005

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