Faculty Profiles - NAGATSUKA Hitoshi

Papers - NAGATSUKA Hitoshi

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Unusual MRI appearance of a lymphoepithelial cyst in the parotid gland Reviewed

Hidenobu Matsuzaki, Naoki Katase, Yoshinobu Yanagi, Marina Hara, Mariko Fujita, Teruhisa Unetsubo, Hitoshi Nagatsuka, Jun Ichi Asaumi

Oral Radiology 28 ( 2 ) 133 - 139 2012.9

Language：English Publishing type：Research paper (scientific journal)

Lymphoepithelial cysts, which are also known as branchial cleft cysts, commonly occur in the lateral cervical region. Lymphoepithelial cysts arising in the parotid gland are rare and must be distinguished from parotid gland tumors. Magnetic resonance imaging (MRI) is useful for diagnosing parotid gland lesions, and MR images of lymphoepithelial cysts typically display a cystic mass that appears homogeneously hypointense on T1-weighted images and homogeneously hyperintense on T2-weighted images. However, some parotid gland tumors that retain fluid in their inner sections show similar MRI findings to lymphoepithelial cysts. Furthermore, lymphoepithelial cysts are sometimes modified by inflammation, and these cases are hard to diagnose. We report the case of a 59-yearold female with a lymphoepithelial cyst that arose in the parotid gland. The cyst had been affected by inflammation and displayed atypical imaging findings, i.e., heterogeneous signal intensity of the liquid component and the presence of a well-enhanced capsule-like structure surrounding the liquid component. In addition, we compare the MRI findings of this case with those of two other cervical lymphoepithelial cysts. © Japanese Society for Oral and Maxillofacial Radiology and Springer 2012.

DOI： 10.1007/s11282-012-0086-0

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Imaging features of adenoid cystic carcinoma of the tongue with dedifferentiated components: A case report Reviewed

Yoshinobu Yanagi, Hidenobu Matsuzaki, Naoki Katase, Tomoo Onoda, Marina Hara, Teruhisa Unetsubo, Hitoshi Nagatsuka, Jun Ichi Asaumi

Oral Radiology 28 ( 2 ) 157 - 165 2012.9

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Adenoid cystic carcinoma (ACC) of the head and neck region is an uncommon epithelial tumor of the major and minor salivary glands. In the oral region, although ACC arising from the minor salivary glands is the second most commonly found tumor in the tongue base, its occurrence in the anterior part of the tongue is rare. Histopathologically, ACC is categorized into three growth patterns (tubular, cribriform, and solid types) and three histologic grades (I-III) that are based on the proportions of these patterns. According to this classification, tubularand cribriform-type ACCs are considered to be lower grade lesions, while solid-type ACCs are considered to be higher grade lesions. A fourth histopathological type has recently been reported by some authors, namely, dedifferentiation or high-grade transformation of ACC. However, very few studies have focused on the imaging features of these ACCs. We report here the case of a 63-year-old female patient with ACC of the tongue with dedifferentiated components, together with the radiological images and pathological features of this ACC. © Japanese Society for Oral and Maxillofacial Radiology and Springer 2012.

DOI： 10.1007/s11282-012-0097-x

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CCN2 production of ameloblastoma is to define the nature of the tumor stroma

Takebe Y, Katase N, Tsujigiwa H, Ito S, Fujii M, Sathi GSA, Harada H, Sasaki A, Nagatsuka H

Journal of Hard Tissue Biology 22 ( 1 ) 155 2012.8

α2アドレナリン受容体作用薬デクスメデトミジンの抗炎症効果

井上美穂, 助川信太郎, 長塚仁, 宮脇卓也

日本歯科医師会雑誌 65 ( 5 ) 688 - 688 2012.8

病理組織学的にエナメル上皮線維腫との鑑別が困難であったimmature odontomaの１例 Reviewed

柴田茜, 志茂剛, 片瀬直樹, 中妻可奈子, 堀切優, 岸本晃治, 西山明慶, 目瀬浩, 長塚仁, 佐々木朗

岡山歯学会雑誌 31 ( 1 ) 15 - 20 2012.6

Central odontogenic fibroma of the jawbone: 2 case reports describing its imaging features and an analysis of its DCE-MRI findings International journal

Marina Hara, Hidenobu Matsuzaki, Naoki Katase, Yoshinobu Yanagi, Teruhisa Unetsubo, Jun Ichi Asaumi, Hitoshi Nagatsuka

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 113 ( 6 ) e51-8 - E58 2012.6

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE INC

Odontogenic fibroma (OF) is a rare nonepithelial benign tumor arising from the odontogenic mesenchymal tissue in the jawbone. OFs are topographically categorized into 2 types, the central type and peripheral type, and are histopathologically divided into the epithelium-poor type and epithelium-rich type. The radiological findings of central OF commonly include a uni- or multilocular radiolucent area with a well-defined margin, which are similar to those of cysts and other benign tumors of the jawbone. Therefore, it is difficult to distinguish OF from these jawbone lesions on radiographs because of their noncharacteristic radiological findings. In this article, we report the cases of 2 patients with central OF who underwent magnetic resonance (MR) examinations and describe the usefulness of dynamic contrast-enhanced MR imaging for diagnosing OF. © 2012 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.oooo.2011.12.013

Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesenchymal stem cells (MSCs) and regeneration of bone from MSC in vivo International journal

Eiki Yamachika, Hidetsugu Tsujigiwa, Masakazu Matsubara, Yasuhisa Hirata, Kenichiro Kita, Kiyofumi Takabatake, Nobuyoshi Mizukawa, Yoshihiro Kaneda, Hitoshi Nagatsuka, Seiji Iida

Journal of Molecular Histology 43 ( 2 ) 223 - 233 2012.4

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Some progress has been made in development of methods to regenerate bone from cultured cells, however no method is put to practical use. Here, we developed methods to isolate, purify, and expand mesenchymal stem cells (MSCs) from mouse compact bone that may be used to regenerate boneinvivo. These cells were maintainedinlong-term culture and were capable of differentiating along multiple lineages, including chondrocyte, osteocyte, and adipocyte trajectories. We used standard cell isolation and culture methods to establish cell cultures from mouse compact bone and bone marrow. Cultures were grown in four distinct media to determine the optimal composition of culture medium for bone-derived MSCs. Putative MSCs were subjected to flow cytometry, alkaline phosphatase assays, immunohistochemical staining, and several differentiation assays to assess cell identity, protein expression, and developmental potential. Finally, we used an in vivo bone formation assay to determine whether putative MSCs were capable of regenerating bone. We found that compact bone of mice was a better source of MCSs than the bone marrow, that growth in plastic flasks served to purify MSCs from hematopoietic cells, and that MSCs grown in basic fibroblast growth factor (bFGF)-conditioned medium were, based on multiple criteria, superior to those grown in leukemia inhibitory factor-conditioned medium. Moreover, we found that the MSCs isolated from compact bone and grown in bFGF-conditioned medium were capable of supporting bone formation in vivo. The methods and results described here haveimplicationsfor understanding MSC biology and for clinical purpose.. © Springer Science+Business Media B.V. 2012.

DOI： 10.1007/s10735-011-9385-8

Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma International journal

Chong Huat Siar, Hitoshi Nagatsuka, Phuu Pwint Han, Rosario Rivera Buery, Hidetsugu Tsujigiwa, Keisuke Nakano, Kok Han Ng, Toshiyuki Kawakami

Journal of Oral Pathology and Medicine 41 ( 4 ) 332 - 339 2012.4

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY

Background: Canonical and non-canonical Wnt signaling pathways modulate diverse cellular processes during embryogenesis and post-natally. Their deregulations have been implicated in cancer development and progression. Wnt signaling is essential for odontogenesis. The ameloblastoma is an odontogenic epithelial neoplasm of enamel organ origin. Altered expressions of Wnts-1, -2, -5a, and -10a are detected in this tumor. The activity of other Wnt members remains unclarified. Materials and Methods: Canonical (Wnts-1, -2, -3, -8a, -8b, -10a, and -10b), non-canonical (Wnts-4, -5a, -5b, -6, 7a, -7b, and -11), and indeterminate groups (Wnts-2b and -9b) were examined immunohistochemically in 72 cases of ameloblastoma (19 unicystic [UA], 35 solid/multicystic [SMA], eight desmoplastic [DA], and 10 recurrent [RA]). Results: Canonical Wnt proteins (except Wnt-10b) were heterogeneously expressed in ameloblastoma. Their distribution patterns were distinctive with some overlap. Protein localization was mainly membranous and/or cytoplasmic. Overexpression of Wnt-1 in most subsets (UA=19/19; SMA=35/35; DA=5/8; RA= 7/10) (P<0.05), Wnt-3 in granular cell variant (n=3/3), and Wnt-8b in DA (n=8/8) was key observations. Wnts-8a and -10a demonstrated enhanced expression in tumoral buddings and acanthomatous areas. Non-canonical and indeterminate Wnts were absent except for limited Wnt-7b immunoreactivity in UA (n=1/19) and SMA (n=1/35). Stromal components expressed variable Wnt positivity. Conclusion: Differential expression of Wnt ligands in different ameloblastoma subtypes suggests that the canonical and non-canonical Wnt pathways are selectively activated or repressed depending on the tumor cell differentiation status. Canonical Wnt pathway is most likely the main transduction pathway while Wnt-1 might be the key signaling molecule involved in ameloblastoma tumorigenesis. © 2011 John Wiley & Sons A/S.

DOI： 10.1111/j.1600-0714.2011.01104.x

Analysis of immunoexpression of common cancer stem cell markers in ameloblastoma International journal

Gulsan Ara Sathi, Ryo Tamamura, Hidetsugu Tsujigiwa, Naoki Katase, Mathieu Lefeuvre, Chong Huat Siar, Hiroyuki Matsuda, Hitoshi Nagatsuka

Experimental and Therapeutic Medicine 3 ( 3 ) 397 - 402 2012.3

Language：English Publishing type：Research paper (scientific journal) Publisher：SPANDIDOS PUBL LTD

Recent studies have established that, in benign tumors, a large number of cancer stem cells are present, which have great implications in tumor development. However, in ameloblastoma, a highly aggressive, locally invasive tumor with a high recurrence rate, whether or not cancer stem-like cells are present remains undetermined. Therefore, in this study we analyzed the protein expression of three candidate stem cell markers in ameloblastoma. Immunohistochemical staining for cancer stem cell (CSC) markers (CD133, CD44 and ABCG2) and for the proliferation marker Ki-67 was performed using 23ameloblastoma cases. In all 23 samples, CD133, CD44 and ABCG2 were expressed. Nine (39.13%) cases showed high expression and 14cases (60.87%) showed low expression for CD133. Twelve of the 23cases (52.17%) showed high expression and 11cases (47.83%) showed low expression for both CD44 and ABCG2, respectively. Ki-67 was mainly expressed in peripheral ameloblast-like cells, suggesting that these cells have a higher degree of differentiation and, therefore, are less likely to contain cancer stem-like cells. On the other hand, cells positive for CSC markers situated at the close proximity to peripheral cells were devoid of Ki-67 and may have the potential to be cancer stem-like cells. After analyzing the correlation between expression of three CSC markers with clinicopathological factors and Ki-67 expression, only CD44 expression was correlated with tumor recurrence (P=0.0391). In conclusion, this study showed various expression patterns of different types of cancer stem cell markers and the presence of candidate CSC-like cells in ameloblastoma, which are possibly involved in cell proliferation, tumor progression and recurrence.

DOI： 10.3892/etm.2011.437

Absence of Dickkopf (Dkk)-3 protein expression is correlated with longer disease-free survival and lower incidence of metastasis in head and neck squamous cell carcinoma International journal

Naoki Katase, Mathieu Lefeuvre, Mehmet Gunduz, Esra Gunduz, Levent Bekir Beder, Reidar Grenman, Masae Fujii, Ryo Tamamura, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka

Oncology Letters 3 ( 2 ) 273 - 280 2012.2

Language：English Publishing type：Research paper (scientific journal) Publisher：SPANDIDOS PUBL LTD

Head and neck squamous cell carcinoma (HNSCC) is one of the most frequently occurring types of cancer worldwide. We focused on the fact that the aberrant function of Wnt/β-catenin signaling is a frequent event in malignancies. Dickkopf (Dkk)-3 is a major negative regulator of Wnt/β catenin signaling, which is a known tumor suppressor and is down regulated in various types of cancer. However, the expression profile of the Dkk-3 protein in HNSCC has not yet been reported. The present study was conducted to investigate Dkk-3 protein expression in 90 cases of HNSCC tissue samples and HNSCC-derived cell lines. In contrast to findings available on other types of cancer, the Western blot analysis revealed that HNSCC cell lines expressed the Dkk-3 protein. In immunohistochemistry, 76 cases (84.4%) out of 90 tissue samples were Dkk-3-positive, whereas only 14 cases (15.6%) were negative. Notably, survival analysis showed that the Dkk-3 (-) group exhibited significantly longer disease-free survival (p=0.038), metastasis-free survival (p=0.013) and longer overall survival (p=0.155). The results showed that the Dkk-3 protein was dominantly expressed and may be involved in carcinogenesis and metastasis in HNSCC. Moreover, the findings suggest that the function of Dkk-3 differs depending on the tissue of origin, and that it may exert an oncogenic function in HNSCC.

DOI： 10.3892/ol.2011.473

Magnetic resonance imaging (MRI) and dynamic MRI evaluation of extranodal non-Hodgkin lymphoma in oral and maxillofacial regions International journal

Hidenobu Matsuzaki, Marina Hara, Yoshinobu Yanagi, Jun Ichi Asaumi, Naoki Katase, Teruhisa Unetsubo, Miki Hisatomi, Hironobu Konouchi, Toshihiko Takenobu, Hitoshi Nagatsuka

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 113 ( 1 ) 126 - 133 2012.1

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE INC

Objective. The purpose of this study was to evaluate the diagnostic value of magnetic resonance imaging (MRI), especially dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), in extranodal non-Hodgkin lymphoma (NHL) of oral and maxillofacial regions. Study design. Thirteen cases with extranodal NHL were examined using MRI. T1-weighted images (T1WI) and T2-weighted images (T2WI) or short TI inversion recovery (STIR) images were obtained in all cases. Contrast-enhanced images and DCEMRI were acquired in 10 and 7 cases, respectively. On DCE-MRIs, we analyzed the parameters as follows: contrast index at maximal contrast enhancement (CImax), maximum contrast index (CI) gain/CImax ratio, and washout ratios (WR300, WR600, and WR900) at 300, 600, and 900 seconds after contrast medium injection. Results. The signal intensity of all lesions was hypointense to isointense on T1WIs and showed variable contrast enhancement patterns. On T2WIs and STIR images, the signal intensity was isointense to hyperintense in almost all cases. Analysis of DCEMRI parameters in extranodal NHLs resulted in the identification of 4 types of CI curves according to CImax and WR: (1) CImax greater than 2.0 and WR900 greater than 40%, (2) CImax greater than 2.0 and WR900 less than 40%, (3) CImax less than 1.5 and WR900 greater than 40%, and (4) CImax less than 1.5 and WR900 greater than 40%. Conclusions. The signal intensities on MRI were not specific to extranodal NHL and resembled those of other tumor types. When CImax was less than 1.5 or WR900 was less than 40%, these parameters contributed to diagnosis in extranodal NHLs. © 2012 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.tripleo.2011.07.038

伊原木聰一郎, 西山明慶, 吉岡徳枝, 兒玉真一, 長塚仁, 目瀬浩, 佐々木朗

日本口腔科学会雑誌 61 ( 1 ) 182 - 182 2012.1

唾液腺腫瘍52例の臨床統計的検討

藤田佑貴, 森谷徳文, 水川展吉, 山近英樹, 喜多憲一郎, 武田斉子, 平田泰久, 長塚仁, 飯田征二

日本口腔科学会雑誌 61 ( 1 ) 215 - 215 2012.1

Bone-healing pattern on critical-sized defects treated by rhPTH

María V. Jammal, Nina F. Pastorino, Carlos M. Abate, Shin Takagi, Hitoshi Nagatsuka, Liliana R. Missana

Journal of Hard Tissue Biology 21 ( 4 ) 443 - 450 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：JOURNAL HARD TISSUE BIOLOGY

Intermittent Recombinant Human Parathyroid Hormone (rhPTH 1-34) is an effective treatment for improving bone mass in patients with osteoporosis; however, its effects on bone regeneration are still unclear. The objective of this study was to evaluate the potential toxicity systemic rhPTH, as well as its ability to regenerate critical-sized defects (CZD) in bone. We used 43 female Wistar rats (body weight, 150 ± 50 g). Critical-sized bone defects in rat calvaria received vehicle alone (Control Group, CG) or daily rhPTH (20 ng/ Kg/day) by subcutaneous injection (Experimental Group, EG). We evaluated bone healing obtained at the 1st, 3rd, and 6th wks post-surgery by biochemical, soft x-ray, histological, and morphometric studies. In the EG, at the 1st and 3rd wks, many areas of focal osteoblast hyperplasia were found on parietal bone. At the 3rd wk, woven and/or lamellar bone, in an organized interconnected trabecular network, showed disrupted mineralization. At the 6th wk, looped bone was found to have formed patterns on parietal bone. New bone formed in the EG showed significant statistical differences (p = 0.023) at the 6th wk. Systemic rhPTH at the dose of 20 ng/Kg/ day was able to stimulate bone formation on rat CZD. Also, pre-existing and new bone showed non-proliferative forms of bone hyperostosis (increased non-neoplastic bone). © 2012 The Hard Tissue Biology Network Association.

DOI： 10.2485/jhtb.21.443

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Application of multiplanar computed tomography and the osirix imaging software for precise analysis of dens invaginatus in the maxillary third molar

Hiroko Kondo, Takayoshi Tobita, Takaaki Ueno, Hitoshi Yoshimura, Naoki Katase, Seigo Ohba, Hitoshi Nagatsuka, Kazuo Sano

Journal of Hard Tissue Biology 21 ( 3 ) 337 - 340 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：JOURNAL HARD TISSUE BIOLOGY

Dens invaginatus is a deep surface invagination of the dentin that is lined by enamel and is easily affected by dental caries, thus resulting in pulpitis and apical periodontitis. This report presents an image analysis of rare dens invaginatus of the maxillary third molar in a 23-year-old man. A radiographic examination showed a radiopaque structure in the dilated roots and a radiolucent area around the root. Tooth extraction and cystectomy were performed. The histological findings showed the tooth to have type III dens invaginatus. This report discusses the application of multiplanar computed tomography (CT) imaging and the free image analysis software (OsiriX) to achieve a more precise evaluation of dens invaginatus. © 2012 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved.

DOI： 10.2485/jhtb.21.337

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Dickkopf (Dkk)-3 and β-catenin expressions increased in the transition from normal oral mucosal to oral squamous cell carcinoma International journal

Masae Fujii, Naoki Katase, Mathieu Lefeuvre, Mehmet Gunduz, Rosario Rivera Buery, Ryo Tamamura, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka

Journal of Molecular Histology 42 ( 6 ) 499 - 504 2011.12

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Dickkopf (Dkk)-3, an inhibitor of the Wnt/β-catenin pathway, is reported as a potential tumor suppressor gene in many cancers. To gain a better comprehension of the mechanisms involved in the carcinogenesis of oral squamous epithelium, protein expression and localization of Dkk-3 and β-catenin was investigated in normal epithelium, dysplasias and squamous cell carcinoma (SCC). An increase in β-catenin and Ki-67 expressions was observed from dysplasias to poorly differentiated SCC. Interestingly, an increase in Dkk-3 positive cells was also noted, which was correlated to the cancer progression step. A change in Dkk-3 localization during the transformation of normal oral epithelium to SCC was clearly observed. Dkk-3 was localized in the cell membrane in normal oral epithelium and in dysplasias, whereas that was localized in both cell membrane and cytoplasm in SCC. These results suggest that Dkk-3 is involved in the carcinogenesis of SCC with a distinct function from those in other cancers. © 2011 Springer Science+Business Media B.V.

DOI： 10.1007/s10735-011-9357-z

Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor

C. H. Siar, Toshiyuki Kawakami, R. R. Buery, K. Nakano, M. Tomida, H. Tsujigiwa, P. P. Han, H. Nagatsuka, K. H. Ng

European Journal of Medical Research 16 ( 11 ) 501 - 506 2011.11

Language：English Publishing type：Research paper (scientific journal) Publisher：BIOMED CENTRAL LTD

Notch signaling is an evolutionarily conserved mechanism that enables adjacent cells to adopt different fates. Ghost cells (GCs) are anucleate cells with homogeneous pale eosinophilic cytoplasm and very pale to clear central areas (previous nucleus sites). Although GCs are present in a variety of odontogenic lesions notably the calcifying cystic odontogenic tumor (CCOT), their nature and process of formation remains elusive. The aim of this study was to investigate the role of Notch signaling in the cell fate specification of GCs in CCOT. Immunohistochemical staining for four Notch receptors (Notch1, Notch2, Notch3 and Notch4) and three ligands (Jagged1, Jagged2 and Delta1) was performed on archival tissues of five CCOT cases. Level of positivity was quantified as negative (0), mild (+), moderate (2+) and strong (3+). Results revealed that GCs demonstrated overexpression for Notch1 and Jagged1 suggesting that Notch1-Jagged1 signaling might serve as the main transduction mechanism in cell fate decision for GCs in CCOT. Protein localizations were largely membranous and/or cytoplasmic. Mineralized GCs also stained positive implicating that the calcification process might be associated with upregulation of these molecules. The other Notch receptors and ligands were weak to absent in GCs and tumoral epithelium. Stromal endothelium and fibroblasts were stained variably positive. © I. Holzapfel Publishers 2011.

NRAS and BRAF mutation frequency in primary oral mucosal melanoma International journal

Rosario Rivera Buery, Chong Huat Siar, Naoki Katase, Mehmet Gunduz, Mathieu Lefeuvre, Masae Fujii, Masahisa Inoue, Kojun Setsu, Hitoshi Nagatsuka

Oncology Reports 26 ( 4 ) 783 - 787 2011.10

Language：English Publishing type：Research paper (scientific journal) Publisher：SPANDIDOS PUBL LTD

Oral mucosal melanoma (OMM) is a fatal sarcoma of unknown etiology. Histological morphology and genetic events are distinct from those of its cutaneous counterpart. Mutation and up-regulation of c-kit has been identified in OMM which may activate downstream molecules such as RAS and RAF. These molecules are involved in the mitogen-activated protein kinase (MAPK) pathway leading to tremendous cell proliferation and survival. NRAS and BRAF mutation and protein expression have been studied in other melanoma subtypes. The purpose of this study was to determine RAS protein expression and NRAS and BRAF mutation in 18 primary OMM cases using immunohistochemistry and mutation analysis. Results showed that RAS is intensely expressed in both in situ and invasive OMMs. However, NRAS mutation was only observed in 2/15 polymerase chain reaction (PCR) amplified cases both of which were silent mutations. On the other hand, BRAF missense mutations were observed only in 1/15 cases with PCR amplification. NRAS and BRAF mutations were independent from previously reported c-kit mutations. The classical V600E BRAF mutation was not found; instead a novel V600L was observed suggesting that the oncogenic event in OMM is different from that in skin melanoma. The low frequency of NRAS and BRAF mutations indicate that these genes are not common, but probable events in OMM pathogenesis, most likely independent of c-kit mutation.

DOI： 10.3892/or.2011.1385

Localization and characterization of lymphatic vessels in oral and cervical squamous cell carcinoma International journal

Masahisa Inoue, Cheng Hsiung Roan, Tomomi Abe, Rosario R. Buery, Hitoshi Nagatsuka, Naoki Katase, Noriyuki Nagai, Kojun Setsu

Experimental and Therapeutic Medicine 2 ( 5 ) 793 - 797 2011.9

Language：English Publishing type：Research paper (scientific journal) Publisher：SPANDIDOS PUBL LTD

Lymph node metastasis is considered a factor in determining the prognosis of squamous cell carcinoma (SCC). Both oral and cervical SCC tumor cells prefer lymph vessels as the route of metastasis. D2-40 is a specific marker of lymphatic endothelial cells. This study clarifies the distribution and characteristics of lymphatic vessels in oral and cervical SCCs. Immunohistochemistry was performed in 20 oral and 20 cervical SCCs (10 non-metastatic and 10 metastatic to lymph nodes) using D2-40, CD31, CD34, CD105 and double staining with D2-40 and keratin. Lymphatic vessel density (LVD) was also determined morphologically. Results showed that lymphatic vessels in both types of SCCs were distributed mainly at the superficial region beneath the epithelium. The LVD in each tumor was significantly higher compared to the corresponding normal mucosa. Moreover, the LVD in lymph node metastasis in each tumor was significantly higher compared to their non-metastatic counterparts. Cancer cell invasion was observed in the lymphatic vessels suggesting the existence of lymph node involvement during metastasis. The new lymphatic vessels that proliferated around the cancer nests in both SCCs have endothelial cell characteristics inferred to be associated with early lymphatic development and initial dissemination of cancer cells.

DOI： 10.3892/etm.2011.277

Dkk-3 protein and mRNA expression in head and neck squamous cell carcinoma and its correlation with the prognosis

片瀬直樹, グンデゥズ・メーメット, 辻極秀次, ルフェーブル・マシュー, シャティ・グルサンアラ, 藤井昌江, 長塚仁

日本癌学会総会記事 70回 171 - 171 2011.9

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