Faculty Profiles - NAGATSUKA Hitoshi

Papers - NAGATSUKA Hitoshi

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Macrognathia secondary to dialysis-related renal osteodystrophy treated successfully by parathyroidectomy

Tsuyoshi Hata, I. Irei, K. Tanaka, H. Nagatsuka, M. Hosoda

International Journal of Oral and Maxillofacial Surgery 35 ( 4 ) 378 - 382 2006.4

Language：English Publishing type：Research paper (scientific journal) Publisher：CHURCHILL LIVINGSTONE

Renal osteodystrophy (ROD) is one of the most common complications affecting patients with chronic renal failure both before and after the initiation of maintenance dialysis, but macrognathia secondary to ROD is rare. Usually, enlarged jaws due to ROD do not return to their normal contours after the treatment of hyperparathyroidism. To the authors' knowledge, this article describes the second case of macrognathia secondary to dialysis-related ROD treated successfully by parathyroidectomy. Immunohistochemical study of the maxilla confirmed that parathyroidectomy could stop maladaptive parathyroid hormone stimulation, which leads not only to the formation of osteoblastic progenitors that become fibroblast-like cells but also to osteoclast formation. © 2005 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.ijom.2005.08.006

Comparison of ready-made and self-setting alginate membranes used as a barrier membrane for guided bone regeneration International journal

Yoshiya Ueyama, Takahiro Koyama, Kunio Ishikawa, Takamitsu Mano, Yukio Ogawa, Hitoshi Nagatsuka, Kazuomi Suzuki

Journal of Materials Science: Materials in Medicine 17 ( 3 ) 281 - 288 2006.3

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

In order to understand the requirements of guided bone regeneration (GBR) involving alginate base self-setting barrier membranes, GBR was performed in the case of bicortical bone defects formed at the tibiae of experimental animals employing self-setting and ready-made alginate membranes. Connective tissue ingress into the bone defects at the skin side of the tibia was observed when GBR was generated utilizing ready-made alginate membrane. In contrast, bone defects were reconstructed with bone tissue when GBR was generated with self-setting alginate membrane formed from aqueous 3% sodium alginate and 3% CaCl2 solutions. The unreacted aqueous sodium alginate solution inherent to self-setting alginate membrane did not inhibit bone tissue regeneration. Rather, callus bone was formed using sodium alginate as the nucleus. However, when GBR was effected with self-setting alginate membrane formed from aqueous 10% CaCl2 solution, membrane was too thick and thus regeneration of bone tissue in the bone cavity was prevented. Therefore, we concluded that self-setting alginate membrane is very useful as a barrier membrane for GBR upon appropriate adjustment of conditions with respect to preparation of alginate membrane. © 2006 Springer Science + Business Media, Inc.

DOI： 10.1007/s10856-006-7315-1

Three cases of adenocarcinoma arising in extremely long-segment Barrett's esophagus International journal

Toshiya Fujiwara, Yoshio Naomoto, Tomoki Yamatsuji, Yasuhiro Shirakawa, Hirofumi Noguchi, Toshiyoshi Fujiwara, Nobuya Oohara, Mehmet Gunduz, Hitoshi Nagatsuka, Manabu Nishie, Hirokazu Uetsuka, Shuji Hamazaki, Noriaki Tanaka

Digestive Diseases and Sciences 51 ( 3 ) 533 - 538 2006.3

Cyclooxygenase 2 expression in otitis media with effusion

Masashi Kakiuchi, Hidetsugu Tsujigiwa, Yorihisa Orita, Hitoshi Nagatsuka, Junko Yoshinobu, Shin Kariya, Shin Ichi Haginomori, Saeko Orita, Kunihiro Fukushima, Mitsuhiro Okano, Noriyuki Nagai, Kazunori Nishizaki

American Journal of Otolaryngology - Head and Neck Medicine and Surgery 27 ( 2 ) 81 - 85 2006.3

Language：English Publishing type：Research paper (scientific journal) Publisher：W B SAUNDERS CO-ELSEVIER INC

Objectives: To study whether cyclooxygenase 2 (COX-2) plays a role in the development of otitis media with effusion (OME). Study design/methods: An experimental model of endotoxin-induced OME was used in healthy BALB/c mice. Solutions of Salmonella typhimurium endotoxin were injected into the middle ears of the mice, and COX-2 expression in the middle ears was studied using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Results: Analysis by RT-PCR showed that COX-2 expression in the middle ear increased in a dose-dependent fashion after injection of endotoxin. ISH demonstrated that COX-2 had distinct expression patterns in the epithelium of middle ears with endotoxin-induced OME. Conclusions: COX-2 expression, however, was not detected in normal middle ears. COX-2 expression may be one factor contributing to the development of OME. © 2006 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.amjoto.2005.07.009

COX-2 induction by heparanase in the progression of breast cancer International journal

Takako Imada, Junji Matsuoka, Tetsuji Nobuhisa, Takaomi Okawa, Toshihiro Murata, Yoko Tabuchi, Yasuhiro Shirakawa, Nobuya Ohara, Mehmet Gunduz, Hitoshi Nagatsuka, Tatsuo Umeoka, Yasuhisa Yamamoto, Motowo Nakajima, Noriaki Tanaka, Yoshio Naomoto

International Journal of Molecular Medicine 17 ( 2 ) 221 - 228 2006.2

Language：English Publishing type：Research paper (scientific journal) Publisher：PROFESSOR D A SPANDIDOS

Breast cancer confined within the lactiferous duct or lobule, without invading the stroma, is called ductal carcinoma in situ (DCIS), whereas breast cancer that has invaded the stroma through the basal membrane is called invasive cancer. Heparanase, an endo-β-D-glucuronidase that specifically degrades heparan sulfate proteoglycans (HSPGs) in the extracellular matrix (ECM), plays an important role when breast cancer cells breach the basal membrane. Recently, we have reported that heparanase is involved in angiogenesis through direct induction of cyclo-oxygenase-2 (COX-2). COX-2 induces vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) and is thus involved in neovascularization. The present study was undertaken to analyze surgically resected breast cancer specimens for heparanase and COX-2 expression, using specimens from 59 patients with invasive cancer and 85 patients with DCIS (including 41 cases of DCIS adjacent to invasive cancer). This study yielded the following results: a) the distribution of heparanase within tumor tissue was identical to that of COX-2; b) heparanase expression was more frequent in invasive cancer than in non-invasive cancer; c) a close positive correlation was noted between heparanase and COX-2 expression (this correlation was particularly strong in cases of invasive cancer); and d) COX-2 expression was always seen in cases positive for heparanase expression. Our results indicate that heparanase expression increases during the progression of breast cancer into invasive cancer, and that this change is accompanied by increased COX-2 expression. They also suggest that heparanase may play a novel role for COX-2 mediated tumor angiogenesis in breast-cancer progression.

DOI： 10.3892/ijmm.17.2.221

Identification of a candidate tumor suppressor gene RHOBTB1 located at a novel allelic loss region 10q21 in head and neck cancer International journal

Levent B. Beder, Mehmet Gunduz, Mamoru Ouchida, Esra Gunduz, Akiko Sakai, Kunihiro Fukushima, Hitoshi Nagatsuka, Sachio Ito, Noriyasu Honjo, Kazunori Nishizaki, Kenji Shimizu

Journal of Cancer Research and Clinical Oncology 132 ( 1 ) 19 - 27 2006.1

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Purpose: Aims of the study are to narrow-down the hotspot region on 10q21 defined by previous genome-wide loss of heterozygosity (LOH) analysis in head and neck squamous cell carcinomas (HNSCC) and to define candidate tumor suppressor genes (TSG) concerned with 10q21. Materials and methods: LOH analysis was carried out with ten polymorphic microsatellite markers. Expression analysis was performed by semi-quantitative RT-PCR, and mutation analysis by PCR and direct sequencing. Results: LOH analysis on 10q21 in 52 HNSCC indicated distinctive and frequent allelic loss at D10S589 (42%). Among flanking genes, we found the RHOBTB1 gene as a candidate TSG, since an intragenic marker demonstrated the highest LOH (44%). Expression analysis revealed down-regulation of RHOBTB1 mRNA in 37% of tumors. Interestingly, all the five tumors that showed decreased expression of RHOBTB1 were accompanied with LOH, supporting the haploinsufficiency and class 2 TSG characteristics of RHOBTB1. No pathogenic mutation of RHOBTB1 was found. Furthermore, another gene within the region, EGR2, was also taken under scope. LOH frequencies around the EGR2 gene were relatively low (23 and 33%). Albeit semi-quantitative expression analysis of EGR2 demonstrated downregulation in 45% of tumor samples, no relation was found between the expression levels and LOH status. Conclusion: Frequent allelic loss and decreased expression of RHOBTB1 suggested that this gene has a role in tumorigenesis of a subset of HNSCC. © Springer-Verlag 2005.

DOI： 10.1007/s00432-005-0033-0

転移リンパ節が鰓性癌に類似した組織像を呈した舌癌の1症例

諸富彰彦, 伊田正道, 内田堅一郎, 福田てる代, 真野隆充, 上山吉哉, 長塚仁

日本口腔外科学会雑誌 52 ( 1 ) 34 - 34 2006.1

A case report of ameloblastoma of the mandible with examination of Notch signaling.

Siar CH, Han NG, Ariff Z, Muraki E, Shimizu T, Tsujigiwa H, Nagatsuka H, Nagai N, Kawakami T

Oral Med Pathol 2006

Frequent Deletion of BRG1 Locus at 19p13 Predicts Recurrence and Previous Cancer History in Oral Squamous Cell Carcinomas

Gunduz Esra, Gunduz Mehmet, Nagatsuka Hitoshi, Beder Levent, Tamamura Ryo, Katase Naoki, Mahmut Naila, Cengiz Beyhan, Fukushima Kunihiro, Nishizaki Kazunori, Shimizu Kenji, Nagai Noriyuki

Journal of hard tissue biology 15 ( 1 ) 20 - 26 2006

Language：English Publisher：The Society of Hard Tissue Biology

Activation of oncogenes and inactivation of tumor suppressor genes (TSGs) is a critical step during carcinogenesis. Inactivation of TSGs occurs through deletion of one allele and mutation in the other allele or decreased mRNA expression. Loss of heterozygosity (LOH) analysis is a sensitive method to detect deletions of specific chromosome regions, which are considered to harbor putative TSGs. By this method we previously demonstrated the frequent deletions of several chromosomal loci and identified candidate TSGs such as ING1, ING3, ING4, Caspase-6 and BRG1 in head and neck cancer. On the other hand, recent researches showed that alterations of chromosomal loci and genes could be used as a predictive marker for the prognosis of the patients, for the behaviour of the tumor and its response to treatments such as chemotherapy and radiotherapy. We recently detected high allelic loss of 19p13 region and identified BRG1 gene as a candidate TSG in 39 oral cancer samples. In the current study, we analyzed the clinicopathological data of the patients and compared with the deletion at BRG1 locus. Our results demonstrated that deletion at BRG1 locus could predict the recurrence, secondary primary cancer, or previous cancer history in oral cancer. Retention of LOH at 19p13 region suggested a high recurrence and secondary primary or previous cancer history. We also detected a higher LOH ratio in cases with smoking and alcohol consumption. The current study suggests that LOH at BRG1 locus could be used as a predictive marker in oral cancer.

DOI： 10.2485/jhtb.15.20

Other Link： http://search.jamas.or.jp/link/ui/2007130839

Differential Distribution of Type IV Collagen α1 to α6 Chains Suggests Distinct Molecular Interaction between the Epithelial and Mesenchymal Components of Benign Odontogenic Tumors

Han Phuu Pwint, Tamamura Ryo, Katase Naoki, Fujii Eiki, Okauchi Mika, Jin Tan, Xiao Jing, Siar Chong Huat, Nagatsuka Hitoshi, Kimoto Suguru, Endo Hiroyasu, Wakami Masanobu, Tamamura Ryo, Okada Hiroyuki, Shimizu Takehiko, Hiratsuka Koichi, Kaneda Takashi, Hirayama Satoshi, Oomine Hirotaka, Matsushima Kiyoshi, Kawai Yasuhiko, Kasai Kazutaka, Shibutani Koh, Kawara Misao, Nasu Ikuo

Journal of hard tissue biology 15 ( 2 ) 46 - 53 2006

Language：English Publisher：The Society of Hard Tissue Biology

Type IV collagen is the principal constituent component of the basement membrane (BM). It have been reported that the alpha chains of type IV collagen [α(IV)] showed temporal and spatial localization pattern in different stages of the developing tooth germ except α3(IV) chain. In this study, we investigated the localization of α(IV) chains in benign odontogenic tumor in comparison with their localization in the cap stage human tooth germ by immunohistochemistry. All the tumor samples studied as well as the tooth germ were negative to α3(IV). In ameloblastoma that resembles the enamel organ of the tooth germ, all types of tumor (follicular, plexiform and desmoplastic) were not reactive to α4(IV) chains similar to the α(IV) chains composition in the BM of outer enamel epithelium and dental lamina. All α(IV) chains except α3(IV) were localized in the tumors in which the odontogenic epithelium is accompanied by odontogenic ectomesenchyme like adenomatoid odontogenic tumor (pseudoglandular spaces), odontoma and ameloblastic fibroma and this is similar to the α(IV) chains composition in the BM of inner enamel epithelium of the tooth germ. From the results, the presence of α4(IV) chains is thought to be important for the differentiation and induction of the dental papilla like tissue formation in odontogenic tumors.

DOI： 10.2485/jhtb.15.46

Other Link： https://jlc.jst.go.jp/DN/JALC/00284408417?from=CiNii

Immunodetection of heparan sulphate and heparanase molecules in benign and malignant odontogenic tumors.

Han PP, Nagatsuka H, Siar CH, Tsujigiwa H, Gunduz M, Tamamura R, Katase N, Nakajima M, Naomoto Y, Nagai N

Oral Med Pathol 2006

Fluoride Supplement Affects Bone Mineralization in Young Rats

Inoue Masahisa, LeGeros Racquel Z., Inoue Miho, Rivera Rosario Santos, Sathi Gulsan Ara, Tsujigiwa Hidetsugu, Nagatsuka Hitoshi, Akita Masahiko, Setsu Kojun

Journal of hard tissue biology 15 ( 2 ) 61 - 64 2006

Language：English Publisher：The Society of Hard Tissue Biology

Fluoride as a supplement can affect the structural integrity of bone. Fluoride that is incorporated in the mineral, substitutes for the hydroxyl group producing hydroxyfluorapatite crystals and presumed to increase bone strength by preventing resorption. Because of this, fluoride therapy has been carried out in clinical trials for the treatment of osteoporosis. Although fluoride renders the mineralized tissues resistant to resorption, the effects of fluoride on bone strength and cortical bone mass specially that of the developing bone has not yet been characterized. The purpose of the study was to analyze the effect of fluoride on developing teeth and bone in young rats through histomorphometry. Sixteen wistar rats were used and divided into two groups; non-fluoride supplemented (control) and fluoride-supplemented groups. Four weeks after, the rats were sacrificed and the mandible and femur were subjected to histological and histomorphometric analyses. No clinical difference was observed between the two groups. The amount of mineralized cartilage was higher in fluoride-supplemented group compared to the control group. However, the amount of new bone was lesser in fluoride-supplemented group compared to the control group. This histomorphometric analysis showed that during endochondral ossification, 10ppm fluoride administration caused some changes in calcified cartilage and bone mineralization in young rats.

DOI： 10.2485/jhtb.15.61

Other Link： https://jlc.jst.go.jp/DN/JALC/00284408431?from=CiNii

Expression of WISP-1 (ccn4), WISP-2 (ccn5) and WISP-3 (ccn6) in Rheumatoid Arthritic Synovium Evaluated by DNA Microarrays

Mori Hiroki, Nishida Keiichiro, Ozaki Toshifumi, Inoue Hajime, Setsu Kojun, Tsujigiwa Hidetsugu, Nagatsuka Hitoshi, Gunduz Mehmet, Nakanishi Tohru

Journal of hard tissue biology 15 ( 3 ) 89 - 95 2006

Language：English Publisher：The Society of Hard Tissue Biology

Rheumatoid disease (RA) is one of the complex diseases that showed multiple progression stages. At first inflammatory response is occurred by self antibodies, and then the proliferation of synoviocytes is abnormally promoted. These synoviocytes invade into articular structures and finally articular cartilage is destroyed. In order to analyze growth-promoting factors in synovial cells from articular tissues of RA, we performed DNA microarray analysis and compared the expression patterns of their mRNA to that of synoviocytes from osteoarthritis (OA). The results showed that the expression of many genes was up-regulated in RA synoviocytes comparing with OA synoviocytes. Among these genes, RT-PCR analysis revealed that Wnt-1-induced secreted protein 2 (WISP-2, CCN5) and protein 3 (WISP-3, CCN6) which are belong to the novel gene family, CCN, were highly up-regulated in RA synoviocytes. In addition, one truncated isoform of WISP-3 which may be related to the ongoing of some type of RA, was detected in RA synoviocytes. Furthermore, immunohistochemical analysis showed that the products of both CCN genes were highly distributed in active synoviocytes of articular tissues of RA. These results indicate that both CCN genes and their products play some roles in the progression of RA.

DOI： 10.2485/jhtb.15.89

Other Link： http://search.jamas.or.jp/link/ui/2007191489

Microcystic adnexal carcinoma in the mental region

Nobuyoshi Mizukawa, Toshio Sugahara, Shin Takagi, Takaaki Ueno, Joji Fukunaga, Hitoshi Nagatsuka

Asian Journal of Oral and Maxillofacial Surgery 18 ( 3 ) 215 - 218 2006

Publishing type：Research paper (scientific journal)

Microcystic adnexal carcinoma is a rare tumour, first described in 1982. Prior to the recognition of microcystic adnexal carcinoma, instances of unusual basal cell carcinoma, sweat gland carcinoma, and trichofolliculoma had been reported. Microcystic adnexal carcinomas are slow growing and demonstrate aggressive local behaviour and a high recurrence rate. The mainstay of treatment is surgical excision using frozen sections to check the peripheral and deep margins. This report is of a microcystic adnexal carcinoma in the mental region of a 69-year-old woman. © 2006 Asian Association of Oral and Maxillofacial Surgeons.

DOI： 10.1016/S0915-6992(06)80021-X

Transforming Growth Factor Beta (TGF-β) Gene Family Members in Developing and Neoplastic Odontogenic Tissues

Heikinheimo Kristiina, Mori Kazuhisa, Nagatsuka Hitoshi, Happonen Risto-Pekka

Journal of hard tissue biology 15,1,1-5? ( 1 ) 1 - 5 2006

Language：English Publisher：The Society of Hard Tissue Biology

During the last decade, several molecules regulating tooth development have been identified (http://bite-it.helsinki.fi/). Especially members of the transforming growth factor beta (TGF-β) supergene family, structurally relates cytokines, are essential for teeth to develop. These include TGF-βs, activin A, bone morphogenetic proteins (BMPs), and their cell surface receptors. In addition, follistatin, an extracellular protein that interacts with activin and BMP signalling, appears to be an important mediator of associated inductive signals. Enhanced or impaired TGF-β/BMP signalling may play a role in odontogenic tumorigenesis. This review focuses on findings of our studies on the roles played by of TGF-β receptors, their ligands and extracellular modifiers during tooth development and in odontogenic tumours.

DOI： 10.2485/jhtb.15.1

Other Link： http://search.jamas.or.jp/link/ui/2007130835

Epigenetic alterations of BRG1 leads to cancer development through its nuclear-cytoplasmic shuttling abnormalities International journal

Esra Gunduz, Mehmet Gunduz, Hitoshi Nagatsuka, Levent Beder, Kadir Demircan, Ryo Tamamura, Omer Faruk Hatipoglu, Naila Mahmut, Naoki Katase, Yoshio Naomoto, Noriyuki Nagai

Medical Hypotheses 67 ( 6 ) 1313 - 1316 2006

Language：English Publishing type：Research paper (scientific journal)

SWI/SNF is a multiprotein chromatin remodeling complex important for gene regulation. BRG1 and its close relative BRM, have ATPase activity necessary for transcriptional regulation by conformational change of nucleosomes. Due to this role on gene expression, several members of SWI/SNF complex including BRG1 and BRM function as a tumor suppressor or negative regulator of cellular proliferation. On the other hand, the shuttling of proteins between nucleus and cytoplasm is strongly involved in the regulation of cell cycle and proliferation. Many of tumor suppressor gene (TSG)s including p53, BRCA1, ING1 play some of their functions through nucleocytoplasmic shuttling. Abnormalities related with this process abrogate the subcellular localization of the TSGs and lead to cancer development. We recently demonstrated BRG1 as a TSG in oral cancer. Our analysis also revealed an interesting finding that one of the splicing forms of BRG1 is selectively lost in cancer tissue as compared to normal counterparts. Our further analysis revealed a putative nuclear retention signal domain for this splicing form. In this article, we speculate the possible mechanism for the inactivation of BRG1 gene in oral cancer through an abnormality in its subcellular localization. © 2006 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.mehy.2006.05.032

Immunohistochemical examination of cytological differentiation in osteosarcomas

Toshiyuki Kawakami, T. Shimizu, A. Kimura, H. Hasegawa, C. H. Siar, K. H. Ng, H. Nagatsuka, N. Nagai, H. Kanda

European Journal of Medical Research 10 ( 11 ) 475 - 479 2005.11

Language：English Publishing type：Research paper (scientific journal) Publisher：I HOLZAPFEL VERLAG GMBH

In this immunohistochemical examination, the expression of Runx2, Notch1, Delta and Osteopontin peptides were detected in neoplastic cells in 10 Japanese cases of osteosarcoma. Immunohistochemically, Runx2 peptide expression appeared in the cytoplasm of almost all neoplastic cells of the 10 cases examined. However, Notch1 peptide expression appeared in the cytoplasm of neoplastic cells in the localized and comparatively well-differentiated area of osteosarcoma, which osteoblastic and chondroblastic containing osteoid and/or chondroid tissues. No expression of Notch1 peptide was detected in the fibroblastic and poorly differentiated areas. Delta peptide appearance was nearly the same pattern of Notch1 peptide. Expression of Osteopontin peptide appeared in almost all cells and the strength expression was shown in the area of comparatively well-differentiated tissues. Therefore, these results suggest that Runx2, Notch1, and Delta peptides are closely related to cytological differentiation or acquisition of tissue specific characteristics in neoplastic cells in osteosarcoma. © I. Holzapfel Publishers 2005.

Role of O⁶-methylguanine-DNA methyltransferase and effect of O⁶-benzylguanine on the anti-tumor activity of cis- diaminedichloroplatinum(II) in oral cancer cell lines International journal

Yu Maki, Jun Murakami, Jun Ichi Asaumi, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Susumu Kokeguchi, Kazuhiro Fukui, Noriko Kawai, Yoshinobu Yanagi, Masahiro Kuroda, Noriaki Tanaka, Nagahide Matsubara, Kanji Kishi

Oral Oncology 41 ( 10 ) 984 - 993 2005.11

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

The DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) modulates the effectiveness of alkylating agents. However, the relationship between MGMT and the sensitivities to other agents has not been explored. In the present study, the association between MGMT expression and the cellular sensitivity to the platinum agent, CDDP was examined in four human oral cancer cell lines. CDDP depleted MGMT protein and mRNA levels in all four cell lines. Two cell lines with low MGMT expression were sensitive to an alkylating agent, N-methyl-N′-nitro-N-nitrosoguanidine and CDDP, whereas two other cell lines with high MGMT expression were resistant to both agents. Furthermore, the addition of the MGMT inhibitor, O6-benzylguanine (O 6-BG), invariably enhanced CDDP sensitivity. CDDP depleted MGMT expression, and CDDP sensitivity was enhanced by O6-BG. These results provide valuable information about the relationship between MGMT expression and CDDP sensitivity in oral cancer chemotherapy. © 2005 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.oraloncology.2005.05.011

Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice International journal

Takako Shimizu, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Norimasa Okafuji, Saburo Kurihara, Noriyuki Nagai, Toshiyuki Kawakami

Angle Orthodontist 75 ( 6 ) 993 - 995 2005.11

Language：English Publishing type：Research paper (scientific journal) Publisher：E H ANGLE EDUCATION RESEARCH FOUNDATION, INC

On the basis of the cellular morphological changes in the cartilaginous area, the mandibular condylar cartilage is histopathologically composed of four different cell layers - fibrous, proliferative, maturative, and hypertrophic. Reaction for Notch1 was present in the hypertrophic cells only. However, Math1 was locally distributed in the hypertrophic layer and partially in the proliferative layer. The expression patterns of Notch1 and Math1 were slightly different. These results suggest that the morphogenesis regulation factors of Notch1 and Math1 may play some role in mandibular condylar cartilage. Positive reactions to osteopontin, as a control, were detected in the cytoplasm of all layers, although they varied from published data. © 2005 by The EH Angle Education and Research Foundation, Inc.

Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells Reviewed

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Mehmet Gunduz, Andrea Rodriguez, Rosario S. Rivera, Racquel Z. Legeros, Miho Inoue, Noriyuki Nagai

Journal of Biomedical Materials Research - Part A 75 ( 1 ) 210 - 215 2005.10

Language：English Publishing type：Research paper (scientific journal)

The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity. © 2005 Wiley Periodicals, Inc.

DOI： 10.1002/jbm.a.30416

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