Faculty Profiles - MURATA Yoshiyuki

Papers - MURATA Yoshiyuki

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Methylglyoxal inhibition of cytosolic ascorbate peroxidase from Nicotiana tabacum. International journal

Md Anamul Hoque, Misugi Uraji, Akiko Torii, Mst Nasrin Akhter Banu, Izumi C Mori, Yoshimasa Nakamura, Yoshiyuki Murata

Journal of biochemical and molecular toxicology 26 ( 8 ) 315 - 21 2012.8

Language：English Publishing type：Research paper (scientific journal)

Methylglyoxal (MG) is one of the aldehydes accumulated in plants under environmental stress. Cytosolic ascorbate peroxidase (cAPX) plays a key role in the protection of cells from oxidative damage by scavenging reactive oxygen species in higher plants. A cDNA encoding cAPX, named NtcAPX, was isolated from Nicotiana tabacum. We characterized recombinant NtcAPX (rNtcAPX) as a fusion protein with glutathione S-transferase to investigate the effects of MG on APX. NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa. The rNtcAPX showed a higher APX activity. MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing K(m) for ascorbate. On the contrary, neither NaCl nor cadmium reduced the activity of APX. The present study suggests that inhibition of APX is in part due to the modification of amino acids by MG.

DOI： 10.1002/jbt.21423

Catalases negatively regulate methyl jasmonate signaling in guard cells. International journal

Rayhanur Jannat, Misugi Uraji, Mohammad Anowar Hossain, Mohammad Muzahidul Islam, Yoshimasa Nakamura, Izumi C Mori, Yoshiyuki Murata

Journal of plant physiology 169 ( 10 ) 1012 - 6 2012.7

Language：English Publishing type：Research paper (scientific journal)

Methyl jasmonate (MeJA)-induced stomatal closure is accompanied by the accumulation of hydrogen peroxide (H₂O₂) in guard cells. In this study, we investigated the roles of catalases (CATs) in MeJA-induced stomatal closure using cat mutants cat2, cat3-1 and cat1 cat3, and the CAT inhibitor, 3-aminotriazole (AT). When assessed with 2',7'-dichlorodihydrofluorescein, the reduction of catalase activity by means of mutations and the inhibitor accumulated higher basal levels of H₂O₂ in guard cells whereas they did not affect stomatal aperture in the absence of MeJA. In contrast, the cat mutations and the treatment with AT potentiated MeJA-induced stomatal closure and MeJA-induced H₂O₂ production. These results indicate that CATs negatively regulate H₂O₂ accumulation in guard cells and suggest that inducible H₂O₂ production rather than constitutive elevation modulates stomatal apertures in Arabidopsis.

DOI： 10.1016/j.jplph.2012.03.006

Methylglyoxal-induced stomatal closure accompanied by peroxidase-mediated ROS production in Arabidopsis. International journal

Tahsina Sharmin Hoque, Misugi Uraji, Wenxiu Ye, Mohammad Anowar Hossain, Yoshimasa Nakamura, Yoshiyuki Murata

Journal of plant physiology 169 ( 10 ) 979 - 86 2012.7

Language：English Publishing type：Research paper (scientific journal)

Methylglyoxal (MG) is an oxygenated short aldehyde and a glycolytic intermediate that accumulates in plants under environmental stresses. Being a reactive α-oxoaldehyde, MG may act as a signaling molecule in plants during stresses. We investigated whether MG induces stomatal closure, reactive oxygen species (ROS) production, and cytosolic free calcium concentration ([Ca²⁺](cyt)) to clarify roles of MG in Arabidopsis guard cells. MG induced production of ROS and [Ca²⁺](cyt) oscillations, leading to stomatal closure. The MG-induced stomatal closure and ROS production were completely inhibited by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), but were not affected by an NAD(P)H oxidase mutation, atrbohD atrbohF. Furthermore, the MG-elicited [Ca²⁺](cyt) oscillations were significantly suppressed by SHAM but not by the atrbohD atrbohF mutation. Neither endogenous abscisic acid nor endogenous methyl jasmonate was involved in MG-induced stomatal closure. These results suggest that intrinsic metabolite MG can induce stomatal closure in Arabidopsis accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca²⁺](cyt) oscillations.

DOI： 10.1016/j.jplph.2012.02.007

Involvement of intracellular oxidative stress-sensitive pathway in phloxine B-induced photocytotoxicity in human T lymphocytic leukemia cells. International journal

Hang Qi, Beiwei Zhu, Naomi Abe, Yuko Shin, Yoshiyuki Murata, Yoshimasa Nakamura

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 50 ( 6 ) 1841 - 7 2012.6

Language：English Publishing type：Research paper (scientific journal)

We investigated the molecular mechanisms underlying phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. In addition to apoptosis-related biochemical events, photo-irradiated PhB generated intracellular reactive oxygen species (ROS), induced phosphorylation of c-Jun-N-terminal kinase (JNK) in an oxidative stress-dependent manner and up-regulated the gene expression of interferon (IFN)-γ, an inducer of diverse apoptosis-related molecules in activated T cells. PhB-induced apoptosis was significantly inhibited by N-acetyl-l-cysteine, but not by catalase, indicating that ROS generation occurred intracellularly, and by SP600125 and AG490, specific inhibitors of JNK and IFN-γ signaling, respectively, confirming their roles in the apoptotic pathway. IFN-γ up-regulation was also inhibited by SP600125, indicating that it was downstream of JNK activation. These results suggest that PhB-induced apoptosis in Jurkat cells partially involves the intracellular oxidative stress-sensitive and T cell-specific IFN-γ pathway. These data present a novel insight into the mechanisms of photocytotoxicity induced by artificial food colorants in human T lymphocytic leukemia cells.

DOI： 10.1016/j.fct.2012.03.011

Optimisation of hydrolysis of purple sea urchin (Strongylocentrotus nudus) gonad by response surface methodology and evaluation of in vitro antioxidant activity of the hydrolysate. International journal

Dayong Zhou, Lei Qin, Beiwei Zhu, Dongmei Li, Jingfeng Yang, Xiuping Dong, Yoshiyuki Murata

Journal of the science of food and agriculture 92 ( 8 ) 1694 - 701 2012.6

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: Hydrolysates prepared from sea urchin (Strongylocentrotus nudus) gonad by enzymatic treatment showed strong 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and reducing power. RESULTS: Hydrolysis of S. nudus gonad by the commercial protease papain was optimised for maximum degree of hydrolysis (DH) and trichloroacetic acid-soluble peptide index (TCA-SPI) using response surface methodology. Results showed that the optimal conditions were the following: temperature of 48.83 °C, pH of 6.92, enzyme-to-substrate ratio of 3143 U g(-1), and substrate concentration of 83.5 g L(-1). Under these conditions, a DH of 27.96 ± 0.54% and a TCA-SPI of 57.32 ± 0.63% were obtained. The hydrolysate prepared in the optimal conditions was fractionated by an ultra-filtration system and the resultant fraction below 10 kDa was found to effectively scavenge hydroxyl radical (EC(50) = 13.29 ± 0.33 mg mL(-1)) and hydrogen peroxide (EC(50) = 16.40 ± 0.37 mg mL(-1)), inhibit lipid peroxidation (EC(50) = 11.05 ± 0.62 mg mL(-1)), chelate Fe(2+) (EC(50) = 7.26 ± 0.44 mg mL(-1)), and protect mice macrophages against death induced by tert-butyl hydroperoxide. CONCLUSION: Hydrolysates prepared from S. nudus gonad have the potential to be applied as natural antioxidant agents.

DOI： 10.1002/jsfa.5534

Effect of γ Irradiation on the Fatty Acid Composition of Soybean and Soybean Oil International journal

MINAMI Ikuko, NAKAMURA Yoshimasa, TODORIKI Setsuko, MURATA Yoshiyuki

Agricultural and Biological Chemistry 76 ( 5 ) 900 - 905 2012.5

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Food irradiation is a form of food processing to extend the shelf life and reduce spoilage of food. We examined the effects of γ radiation on the fatty acid composition, lipid peroxidation level, and antioxidative activity of soybean and soybean oil which both contain a large amount of unsaturated fatty acids. Irradiation at 10 to 80 kGy under aerobic conditions did not markedly change the fatty acid composition of soybean. While 10-kGy irradiation did not markedly affect the fatty acid composition of soybean oil under either aerobic or anaerobic conditions, 40-kGy irradiation considerably altered the fatty acid composition of soybean oil under aerobic conditions, but not under anaerobic conditions. Moreover, 40-kGy irradiation produced a significant amount of trans fatty acids under aerobic conditions, but not under anaerobic conditions. Irradiating soybean oil induced lipid peroxidation and reduced the radical scavenging activity under aerobic conditions, but had no effect under anaerobic conditions. These results indicate that the fatty acid composition of soybean was not markedly affected by radiation at 10 kGy, and that anaerobic conditions reduced the degradation of soybean oil that occurred with high doses of γ radiation.

DOI： 10.1271/bbb.110859

Other Link： https://jlc.jst.go.jp/DN/JALC/10000482249?from=CiNii

Mechanisms of the Selenium Tolerance of the Arabidopsis thaliana Knockout Mutant of Sulfate Transporter SULTR1;2 International journal

OHNO Misao, URAJI Misugi, SHIMOISHI Yasuaki, MORI Izumi C., NAKAMURA Yoshimasa, MURATA Yoshiyuki

Agricultural and Biological Chemistry 76 ( 5 ) 993 - 998 2012.5

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

We investigated the mechanism of selenium (Se) tolerance using an Arabidopsis thaliana knockout mutant of a sulfate transporter, sultr1;2. Se stress inhibited plant growth, decreased chlorophyll contents, and increased protein oxidation and lipid peroxidation in the wild type, whereas the sultr1;2 mutation mitigated damage of these forms, indicating that sultr1;2 is more tolerant of Se than the wild type is. The accumulation of symplastic Se was suppressed in sultr1;2 as compared to the wild type, and the chemical speciation of Se in the mutant was different from that in the wild type. Regardless of Se stress, the activities of ascorbate peroxidase, catalase, and peroxidase in the mutant were higher than in the wild type, while the activity of superoxide dismutase in the mutant was the same as in the wild type. These results suggest that the sultr1;2 mutation confers Se tolerance on Arabidopsis by decreasing symplastic Se and maintaining antioxidant enzyme activities.

DOI： 10.1271/bbb.111000

Other Link： https://jlc.jst.go.jp/DN/JALC/10000482430?from=CiNii

Cooperative Function of PLD delta and PLD alpha 1 in Abscisic Acid-Induced Stomatal Closure in Arabidopsis Reviewed International journal

Misugi Uraji, Takeshi Katagiri, Eiji Okuma, Wenxiu Ye, Mohammad Anowar Hossain, Choji Masuda, Aya Miura, Yoshimasa Nakamura, Izumi C. Mori, Kazuo Shinozaki, Yoshiyuki Murata

PLANT PHYSIOLOGY 159 ( 1 ) 450 - 460 2012.5

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Phospholipase D (PLD) is involved in responses to abiotic stress and abscisic acid (ABA) signaling. To investigate the roles of two Arabidopsis (Arabidopsis thaliana) PLDs, PLD alpha 1 and PLD delta, in ABA signaling in guard cells, we analyzed ABA responses in guard cells using Arabidopsis wild type, pld alpha 1 and pld delta single mutants, and a pld alpha 1 pld delta double mutant. ABA-induced stomatal closure was suppressed in the pld alpha 1 pld delta double mutant but not in the pld single mutants. The pld alpha 1 and pld delta mutations reduced ABA-induced phosphatidic acid production in epidermal tissues. Expression of either PLD alpha 1 or PLD delta complemented the double mutant stomatal phenotype. ABA-induced stomatal closure in both pld alpha 1 and pld delta single mutants was inhibited by a PLD inhibitor (1-butanol), suggesting that both PLD alpha 1 and PLD delta function in ABA-induced stomatal closure. During ABA-induced stomatal closure, wild-type guard cells accumulate reactive oxygen species and nitric oxide and undergo cytosolic alkalization, but these changes are reduced in guard cells of the pld alpha 1 pld delta double mutant. Inward-rectifying K+ channel currents of guard cells were inhibited by ABA in the wild type but not in the pld alpha 1 pld delta double mutant. ABA inhibited stomatal opening in the wild type and the pld delta mutant but not in the pld alpha 1 mutant. In wild-type rosette leaves, ABA significantly increased PLD delta transcript levels but did not change PLD alpha 1 transcript levels. Furthermore, the pld alpha 1 and pld delta mutations mitigated ABA inhibition of seed germination. These results suggest that PLD alpha 1 and PLD delta cooperate in ABA signaling in guard cells but that their functions do not completely overlap.

DOI： 10.1104/pp.112.195578

Inhibitory Effects of Methylglyoxal on Light-Induced Stomatal Opening and Inward K^+ Channel Activity in Arabidopsis International journal

HOQUE Tahsina Sharmin, OKUMA Eiji, URAJI Misugi, FURUICHI Takuya, SASAKI Takayuki, HOQUE Anamul, NAKAMURA Yoshimasa, MURATA Yoshiyuki

Bioscience, Biotechnology, and Biochemistry 76 ( 3 ) 617 - 619 2012.3

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Methylglyoxal (MG) is a reactive aldehyde derived by glycolysis. In Arabidopsis, MG inhibited light-induced stomatal opening in a dose-dependent manner. It significantly inhibited both inward-rectifying potassium (K_in) channels in guard-cell protoplasts and an Arabidopsis K_in channel, KAT1, heterologously expressed in Xenopus oocytes. Thus it appears that MG inhibition of stomatal opening involves MG inhibition of K⁺ influx into guard cells.

DOI： 10.1271/bbb.110885

Other Link： http://search.jamas.or.jp/link/ui/2012301713

Regulation of a Proteinaceous Elicitor-induced Ca2+ Influx and Production of Phytoalexins by a Putative Voltage-gated Cation Channel, OsTPC1, in Cultured Rice Cells Reviewed International journal

Haruyasu Hamada, Takamitsu Kurusu, Eiji Okuma, Hiroshi Nokajima, Masahiro Kiyoduka, Tomoko Koyano, Yoshimi Sugiyama, Kazunori Okada, Jinichiro Koga, Hikaru Saji, Akio Miyao, Hirohiko Hirochika, Hisakazu Yamane, Yoshiyuki Murata, Kazuyuki Kuchitsu

JOURNAL OF BIOLOGICAL CHEMISTRY 287 ( 13 ) 9931 - 9939 2012.3

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Pathogen/microbe-or plant-derived signaling molecules (PAMPs/MAMPs/DAMPs) or elicitors induce increases in the cytosolic concentration of free Ca2+ followed by a series of defense responses including biosynthesis of antimicrobial secondary metabolites called phytoalexins; however, the molecular links and regulatory mechanisms of the phytoalexin biosynthesis remains largely unknown. A putative voltage-gated cation channel, OsTPC1 has been shown to play a critical role in hypersensitive cell death induced by a fungal xylanase protein (TvX) in suspension-cultured rice cells. Here we show that TvX induced a prolonged increase in cytosolic Ca2+, mainly due to a Ca2+ influx through the plasma membrane. Membrane fractionation by two-phase partitioning and immunoblot analyses revealed that OsTPC1 is localized predominantly at the plasma membrane. In retrotransposon- insertional Ostpc1 knock-out cell lines harboring a Ca2+-sensitive photoprotein, aequorin, TvX-induced Ca2+ elevation was significantly impaired, which was restored by expression of OsTPC1. TvX-induced production of major diterpenoid phytoalexins and the expression of a series of diterpene cyclase genes involved in phytoalexin biosynthesis were also impaired in the Ostpc1 cells. Whole cell patch clamp analyses of OsTPC1 heterologously expressed in HEK293T cells showed its voltage-dependent Ca2+-permeability. These results suggest that OsTPC1 plays a crucial role in TvX-induced Ca2+ influx as a plasma membrane Ca2+ permeable channel consequently required for the regulation of phytoalexin biosynthesis in cultured rice cells.

DOI： 10.1074/jbc.M111.337659

α-Tocopherol Sensitizes Human Leukemia HL-60 Cells to Apoptosis Induced by Benzyl Isothiocyanate International journal

ABE Naomi, SHIMIZU Tomomi, MIYOSHI Noriyuki, MURATA Yoshiyuki, NAKAMURA Yoshimasa

Agricultural and Biological Chemistry 76 ( 2 ) 381 - 383 2012.2

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Pre-treatment with α-tocopherol (α-Toc) potentiated cytotoxicity induction by benzyl isothiocyanate (BITC). Biochemical events related to apoptosis, such as DNA ladder formation and caspase-3 activation, were also enhanced by α-Toc. These results suggest a significant role of the caspase-3 pathway in apoptosis induction regulated by α-Toc in combination.

DOI： 10.1271/bbb.110665

Other Link： https://jlc.jst.go.jp/DN/JALC/00389593438?from=CiNii

Cytotoxicity of benzyl isothiocyanate in normal renal proximal tubular cells and its modulation by glutathione. International journal

Naomi Abe, Masashi Okuhira, Chiharu Tsutsui, Yoshiyuki Murata, Yoshimasa Nakamura

Journal of agricultural and food chemistry 60 ( 7 ) 1887 - 92 2012.2

Language：English Publishing type：Research paper (scientific journal)

In the present study, we examined the toxicity of benzyl ITC (BITC) and its urinary mercapturic acid metabolite (BITC-NAC), using a normal renal proximal tubular cell line, pig LLC-PK1. BITC increased cell death with an IC(50) value of about 7 μM, whereas the cytotoxic effect of BITC-NAC was five times weaker than that of BITC. We observed a significant necrosis of the compounds on LLC-PK1 cells with oxidative stress. In the presence of 5 mM glutathione (GSH), comparable to physiological levels, the cytotoxicity of BITC-NAC as well as BITC was significantly reduced. Furthermore, the increase in intracellular GSH levels by pretreatment with NAC before the BITC treatment resulted in inhibition of the BITC-induced necrotic events as well as intracellular oxidative stress. These results suggest that GSH is a determinant of cellular resistance against the BITC-mediated and oxidative stress-dependent cytotoxicity in renal proximal tubular cells.

DOI： 10.1021/jf2052042

FIA functions as an early signal component of abscisic acid signal cascade in Vicia faba guard cells Reviewed International journal

Yusuke Sugiyama, Misugi Uraji, Megumi Watanabe-Sugimoto, Eiji Okuma, Shintaro Munemasa, Yasuaki Shimoishi, Yoshimasa Nakamura, Izumi C. Mori, Sumio Iwai, Yoshiyuki Murata

JOURNAL OF EXPERIMENTAL BOTANY 63 ( 3 ) 1357 - 1365 2012.2

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

An abscisic acid (ABA)-insensitive Vicia faba mutant, fia (fava bean impaired in ABA-induced stomatal closure) had previously been isolated. In this study, it was investigated how FIA functions in ABA signalling in guard cells of Vicia faba. Unlike ABA, methyl jasmonate (MeJA), H2O2, and nitric oxide (NO) induced stomatal closure in the fia mutant. ABA did not induce production of either reactive oxygen species or NO in the mutant. Moreover, ABA did not suppress inward-rectifying K+ (K-in) currents or activate ABA-activated protein kinase (AAPK) in mutant guard cells. These results suggest that FIA functions as an early signal component upstream of AAPK activation in ABA signalling but does not function in MeJA signalling in guard cells of Vicia faba.

DOI： 10.1093/jxb/err369

Effects of Exogenous Proline and Glycinebetaine on the Salt Tolerance of Rice Cultivars International journal

SOBAHAN Muhammad Abdus, AKTER Nasima, OHNO Misao, OKUMA Eiji, HIRAI Yoshihiko, MORI Izumi C., NAKAMURA Yoshimasa, MURATA Yoshiyuki

Agricultural and Biological Chemistry 76 ( 8 ) 1568 - 1570 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Salinity significantly increased trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS) uptake and decreased the K⁺/Na⁺ ratio in salt-sensitive rice (Nipponbare) but did not markedly in salt-tolerant rice (Pokkali). Proline and glycinebetaine (betaine) suppressed the increase in PTS uptake and the decrease in the K⁺/Na⁺ ratio in Nipponbare, but did not affect PTS uptake or the K⁺/Na⁺ ratio in Pokkali.

DOI： 10.1271/bbb.120233

Isolation and characterization of pepsin-soluble collagen from abalone (Haliotis discus hannai) gastropod muscle part ii

Xiuping Dong, Qixin Yuan, Hang Qi, Jingfeng Yang, Beiwei Zhu, Dayong Zhou, Yoshiyuki Murata, Wenxiu Ye

Food Science and Technology Research 18 ( 2 ) 271 - 278 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Food Science and Technology

Abalone (Haliotis discus hannai) gastropod muscle was divided into Part I (the center part of gastropod muscle) and Part II (the transforming and virginal part of gastropod muscle). Pepsin-soluble collagen was extracted from abalone gastropod muscle part II (PSCAGM-II) with yield of 8.7%. SDSPAGE and amino acid composition indicated that the PSCAGM-II contained α1 and α2 chains and might be classified as type I collagen. PSCAGM-II exhibited a maximum absorbance at 232 nm, but little absorbance near to 280 nm. FTIR investigations showed the existence of helical arrangements of collagen. PSCAGM-II triple helical stereochemical structure was destroyed when the treated temperature up to 35°C for 10 min. Denatured temperature detected by the viscometer was 22.9°C when incubated for 20 min. Study on the properties and thermal stability of PSC from abalone gastropod muscle has certain directive significance for the abalone processing industry.

DOI： 10.3136/fstr.18.271

Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells. International journal

Rayhanur Jannat, Misugi Uraji, Miho Morofuji, Mohammad Muzahidul Islam, Rachel E Bloom, Yoshimasa Nakamura, C Robertson McClung, Julian I Schroeder, Izumi C Mori, Yoshiyuki Murata

Journal of plant physiology 168 ( 16 ) 1919 - 26 2011.11

Language：English Publishing type：Research paper (scientific journal)

Reactive oxygen species (ROS), including hydrogen peroxide (H(2)O(2)), are among the important second messengers in abscisic acid (ABA) signaling in guard cells. In this study, to investigate specific roles of H(2)O(2) in ABA signaling in guard cells, we examined the effects of mutations in the guard cell-expressed catalase (CAT) genes, CAT1 and CAT3, and of the CAT inhibitor 3-aminotriazole (AT) on stomatal movement. The cat3 and cat1 cat3 mutations significantly reduced CAT activities, leading to higher basal level of H(2)O(2) in guard cells, when assessed by 2',7'-dichlorodihydrofluorescein, whereas they did not affect stomatal aperture size under non-stressed condition. In addition, AT-treatment at concentrations that abolish CAT activities, showed trivial affect on stomatal aperture size, while basal H(2)O(2) level increased extensively. In contrast, cat mutations and AT-treatment potentiated ABA-induced stomatal closure. Inducible ROS production triggered by ABA was observed in these mutants and wild type as well as in AT-treated guard cells. These results suggest that ABA-inducible cytosolic H(2)O(2) elevation functions in ABA-induced stomatal closure, while constitutive increase of H(2)O(2) do not cause stomatal closure.

DOI： 10.1016/j.jplph.2011.05.006

Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis International journal

Eiji Okuma, Md. Sarwar Jahan, Shintaro Munemasa, Mohammad Anowar Hossain, Daichi Muroyama, Mohammad Mahbub Islam, Ken’ichi Ogawa, Megumi Watanabe-Sugimoto, Yoshimasa Nakamura, Yasuaki Shimoishi, Izumi C. Mori, Yoshiyuki Murata

Journal of Plant Physiology 168 ( 17 ) 2048 - 2055 2011.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca(2+)-permeable channel currents by ABA or oscillation of the cytosolic free Ca(2+) concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca(2+) oscillation in ABA signal pathway of Arabidopsis guard cells.

DOI： 10.1016/j.jplph.2011.06.002

Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis International journal

MD. ATIQUR RAHMAN KHOKON, MD. SARWAR JAHAN, TANIYA RAHMAN, MOHAMMAD ANOWAR HOSSAIN, DAICHI MUROYAMA, IKUKO MINAMI, SHINTARO MUNEMASA, IZUMI C. MORI, YOSHIMASA NAKAMURA, YOSHIYUKI MURATA

Plant, Cell & Environment 34 ( 11 ) 1900 - 1906 2011.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca(2+) . AITC-induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC-induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2-2 and methyl jasmonate (MeJA)-deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC-induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata.

DOI： 10.1111/j.1365-3040.2011.02385.x

K252a-sensitive protein kinases but not okadaic acid-sensitive protein phosphatases regulate methyl jasmonate-induced cytosolic Ca2+ oscillation in guard cells of Arabidopsis thaliana International journal

Mohammad Anowar Hossain, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Journal of Plant Physiology 168 ( 16 ) 1901 - 1908 2011.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Methyl jasmonate (MeJA) induces stomatal closure similar to abscisic acid (ABA), and MeJA signaling in guard cells shares some signal components with ABA signaling. As part of this process, MeJA as well as ABA induce the elevation and oscillation of cytosolic free-calcium concentrations ([Ca(2+)](cyt)) in guard cells. While abscisic acid-induced [Ca(2+)](cyt) oscillation has been extensively studied, MeJA-induced [Ca(2+)](cyt) oscillation is less well understood. In this study, we investigated the effects of K252a (a broad-range protein kinase inhibitor) and okadaic acid (OA, a protein phosphatase 1 and 2A inhibitor) on MeJA-induced [Ca(2+)](cyt) oscillation in guard cells of Arabidopsis thaliana ecotype Columbia expressing the Ca(2+) reporter yellow cameleon 3.6. The protein kinase inhibitor K252a abolished MeJA-induced stomatal closure and reduced MeJA-elicited [Ca(2+)](cyt) oscillation. The protein phosphatase inhibitor OA, on the other hand, did not inhibit these processes. These results suggest that MeJA signaling involves activation of K252a-sensitive protein kinases upstream of [Ca(2+)](cyt) oscillation but not activation of an OA-sensitive protein phosphatase in guard cells of A. thaliana ecotype Columbia.

DOI： 10.1016/j.jplph.2011.05.004

The Roles of CATALASE2 in Abscisic Acid Signaling in Arabidopsis Guard Cells International journal

JANNAT Rayhanur, URAJI Misugi, MOROFUJI Miho, HOSSAIN Mohammad Anowar, ISLAM Mohammad Muzahidul, NAKAMURA Yoshimasa, MORI Izumi C., MURATA Yoshiyuki

Agricultural and Biological Chemistry 75 ( 10 ) 2034 - 2036 2011.10

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

We investigated the roles of catalase (CAT) in abscisic acid (ABA)-induced stomatal closure using a cat2 mutant and an inhibitor of CAT, 3-aminotriazole (AT). Constitutive reactive oxygen species (ROS) accumulation due to the CAT2 mutation and AT treatment did not affect stomatal aperture in the absence of ABA, whereas ABA-induced stomatal closure, ROS production, and [Ca²⁺]_cyt oscillation were enhanced.

DOI： 10.1271/bbb.110344

Other Link： https://jlc.jst.go.jp/DN/JALC/00380217661?from=CiNii

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