Faculty Profiles - MURATA Yoshiyuki

Papers - MURATA Yoshiyuki

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Quantitative Analysis of the Effects of Diffusates from Plant Roots on the Hatching of Meloidogyne chitwoodi from Young and Senescing Host Plants International journal

KHOKON Md. Atiqur Rahman, OKUMA Eiji, RAHMAN Taniya, WESEMAEL Wim M. L., MURATA Yoshiyuki, MOENS Maurice

Agricultural and Biological Chemistry 73 ( 10 ) 2345 - 2347 2009.10

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

We analyzed the effects of diffusates from six different crops on the egg hatching of Meloidogyne chitwoodi collected from young and senescing tomato plants using a non-linear model. Diffusates only from tomato and carrot significantly delayed the hatching of eggs from young plants, but did not do so from senescing plants, suggesting that delay of hatching depends on the origin of diffusates and host plant age.

DOI： 10.1271/bbb.90392

Other Link： https://jlc.jst.go.jp/DN/JALC/00338802222?from=CiNii

Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells. International journal

Mohammad Muzahidul Islam, Md Anamul Hoque, Eiji Okuma, Mst Nasrin Akhter Banu, Yasuaki Shimoishi, Yoshimasa Nakamura, Yoshiyuki Murata

Journal of plant physiology 166 ( 15 ) 1587 - 97 2009.10

Language：English Publishing type：Research paper (scientific journal)

Environmental stress, including heavy metal stress, can cause oxidative damage to plants. Up-regulation of the antioxidant defense system induced by proline and glycinebetaine (betaine) alleviates the damaging effects of oxidative stress in plants. Here, we investigated the protective effects of exogenously applied proline and betaine on growth, accumulation of proline and betaine, lipid peroxidation and activity of antioxidant enzymes in cultured tobacco Bright Yellow-2 (BY-2) cells exposed to cadmium (Cd) stress. Cadmium stress (at 100 microM Cd) caused a significant inhibition of the growth of BY-2 cells, and both proline and betaine significantly mitigated this inhibition. In addition, the mitigating effect of proline was more pronounced than that of betaine. Cadmium stress leads to an accumulation of Cd and endogenous proline in cultured cells, increased lipid peroxidation and peroxidase (POX) activity, and decreased activity of superoxide dismutase (SOD) and catalase (CAT). Exogenous application of proline resulted in a decrease in lipid peroxidation and an increase in SOD and CAT activities without reducing Cd contents under Cd stress, while application of betaine resulted in a decrease in lipid peroxidation and an increase in CAT activity with reducing Cd accumulation. Furthermore, exogenous proline and betaine intensified the accumulation of proline and betaine in Cd-stressed BY-2 cells, respectively. The present study suggests that proline and betaine confer tolerance to Cd stress in tobacco BY-2 cells by different mechanisms.

DOI： 10.1016/j.jplph.2009.04.002

JNK-dependent NFATc1 pathway positively regulates IL-13 gene expression induced by (-)-epigallocatechin-3-gallate in human basophilic KU812 cells. International journal

Haitao Wu, Hang Qi, Dai Iwasaki, Beiwei Zhu, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

Free radical biology & medicine 47 ( 7 ) 1028 - 38 2009.10

Language：English Publishing type：Research paper (scientific journal)

(-)-Epigallocatechin-3-gallate (EGCG) has been reported to possess a wide range of biological and pharmacological properties. In this study, we investigated the effects of EGCG on IL-13 gene expression in human basophilic KU812 cells. The IL-13 mRNA expression level was dose-dependently increased by treatment with EGCG (5-20 microM) for 1 h and additional incubation in a medium for 23 h. EGCG significantly increased the intracellular peroxide level as detected by the peroxide-sensitive probe 2',7'-dichlorodihydrofluorescein diacetate. A pharmacological experiment using catalase and a structure-activity relationship study revealed that the exogenously produced H(2)O(2) significantly, but partially, contributed to the IL-13 expression as well as the intracellular oxidative status. Furthermore, EGCG at the concentration required for IL-13 up-regulation activated c-Jun NH(2)-terminal kinase (JNK), but not extracellular signal-regulated protein kinase or p38 mitogen-activated protein kinase in KU812 cells. Transfection of a JNK-specific siRNA as well as treatment with a JNK-specific inhibitor, SP600125, significantly reduced the EGCG-induced IL-13 mRNA expression, by 47.1 and 44.6%, respectively. In addition, we observed the nuclear translocation, mRNA up-regulation, and activation of DNA binding with the IL-13 promoter of nuclear factor of activated T cells (NFATc1) in the EGCG-treated cells. These data provide biological evidence that EGCG induces IL-13 mRNA expression via the JNK-dependent NFATc1 pathway in KU812 cells.

DOI： 10.1016/j.freeradbiomed.2009.07.011

Exogenous Proline and Glycinebetaine Suppress Apoplastic Flow to Reduce Na^+ Uptake in Rice Seedlings International journal

SOBAHAN Muhammad Abdus, ARIAS Carlos Raul, OKUMA Eiji, SHIMOISHI Yasuaki, NAKAMURA Yoshimasa, HIRAI Yoshihiko, MORI Izumi C., MURATA Yoshiyuki

Agricultural and Biological Chemistry 73 ( 9 ) 2037 - 2042 2009.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

The application of exogenous proline and glycinebetaine (betaine) confers salt tolerance on plants under salt stress. The effects of exogenous proline and betaine on apoplastic flow in rice plants under saline conditions were investigated using trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS), an apoplastic tracer. Rice plants took up more PTS under light conditions than under dark conditions. Salt stress increased PTS uptake and Na⁺ content of rice leaves, but did not affect K⁺ content, resulting in a lower K⁺/Na⁺ ratio. Addition of proline or betaine to the saline medium suppressed Na⁺-induced PTS uptake and Na⁺ accumulation, while the K⁺ content was slightly increased, which led to a high K⁺/Na⁺ ratio under saline conditions. These results suggest that exogenous proline and betaine suppressed Na⁺-enhanced apoplastic flow to reduce Na⁺ uptake in rice plants.

DOI： 10.1271/bbb.90244

Other Link： https://jlc.jst.go.jp/DN/JALC/00337018792?from=CiNii

Calcium elevation-dependent and attenuated resting calcium-dependent abscisic acid induction of stomatal closure and abscisic acid-induced enhancement of calcium sensitivities of S-type anion and inward-rectifying K+ channels in Arabidopsis guard cells Reviewed International journal

Robert S. Siegel, Shaowu Xue, Yoshiyuki Murata, Yingzhen Yang, Noriyuki Nishimura, Angela Wang, Julian I. Schroeder

PLANT JOURNAL 59 ( 2 ) 207 - 220 2009.7

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

P>Stomatal closure in response to abscisic acid depends on mechanisms that are mediated by intracellular [Ca2+] ([Ca2+](i)), and also on mechanisms that are independent of [Ca2+](i) in guard cells. In this study, we addressed three important questions with respect to these two predicted pathways in Arabidopsis thaliana. (i) How large is the relative abscisic acid (ABA)-induced stomatal closure response in the [Ca2+](i)-elevation-independent pathway? (ii) How do ABA-insensitive mutants affect the [Ca2+](i)-elevation-independent pathway? (iii) Does ABA enhance (prime) the Ca2+ sensitivity of anion and inward-rectifying K+ channel regulation? We monitored stomatal responses to ABA while experimentally inhibiting [Ca2+](i) elevations and clamping [Ca2+](i) to resting levels. The absence of [Ca2+](i) elevations was confirmed by ratiometric [Ca2+](i) imaging experiments. ABA-induced stomatal closure in the absence of [Ca2+](i) elevations above the physiological resting [Ca2+](i) showed only approximately 30% of the normal stomatal closure response, and was greatly slowed compared to the response in the presence of [Ca2+](i) elevations. The ABA-insensitive mutants ost1-2, abi2-1 and gca2 showed partial stomatal closure responses that correlate with [Ca2+](i)-dependent ABA signaling. Interestingly, patch-clamp experiments showed that exposure of guard cells to ABA greatly enhances the ability of cytosolic Ca2+ to activate S-type anion channels and down-regulate inward-rectifying K+ channels, providing strong evidence for a Ca2+ sensitivity priming hypothesis. The present study demonstrates and quantifies an attenuated and slowed ABA response when [Ca2+](i) elevations are directly inhibited in guard cells. A minimal model is discussed, in which ABA enhances (primes) the [Ca2+](i) sensitivity of stomatal closure mechanisms.

DOI： 10.1111/j.1365-313X.2009.03872.x

Myrosinases, TGG1 and TGG2, Redundantly Function in ABA and MeJA Signaling in Arabidopsis Guard Cells

ISLAM Mohammad Mahbub, TANI Chiharu, WATANABE SUGIMOTO Megumi, URAJI Misugi, JAHAN Md. Sarwar, MASUDA Choji, NAKAMURA Yoshimasa, MORI Izumi C., MURATA Yoshiyuki

Plant and Cell Physiology 50 ( 6 ) 1171 - 1175 2009.6

Language：English Publishing type：Research paper (scientific journal)

Thioglucoside glucohydrolase (myrosinase), TGG1, is a strikingly abundant protein in Arabidopsis guard cells. We investigated responses of tgg1-3, tgg2-1 and tgg1-3 tgg2-1 mutants to abscisic acid (ABA) and methyl jasmonate (MeJA) to clarify whether two myrosinases, TGG1 and TGG2, function during stomatal closure. ABA, MeJA and H(2)O(2) induced stomatal closure in wild type, tgg1-3 and tgg2-1, but failed to induce stomatal closure in tgg1-3 tgg2-1. All mutants and wild type showed Ca(2+)-induced stomatal closure and ABA-induced reactive oxygen species (ROS)production. A model is discussed in which two myrosinases redundantly function downstream of ROS production and upstream of cytosolic Ca(2+) elevation in ABA and MeJA signaling in guard cells.

DOI： 10.1093/pcp/pcp066

Induction of Apoptosis by β-Carotene and Dimethyl Tetrasulfide Assisted by UVA Irradiation in HL-60 Cells International journal

ZHANG Gongliang, WU Haitao, ZHU Beiwei, SHIMOISHI Yasuaki, NAKAMURA Yoshimasa, MURATA Yoshiyuki

Agricultural and Biological Chemistry 73 ( 5 ) 1014 - 1020 2009.5

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Cellular phototoxicity induced by UVA irradiation and its potential application to therapy has been reported. In the present study, the induction of apoptosis induced by β-carotene and dimethyl tetrasulfide (Me₂S₄) assisted by UVA irradiation in HL-60 cells was assessed. β-carotene assisted by UVA significantly decreased the cell viability and induced DNA fragmentation in HL-60 cells. Me₂S₄ combined with β-carotene and assisted by UVA significantly inhibited the cell viability, and enhanced the caspase-3 activity which was completely inhibited by N-acety-<small>L</small>-cysteine. β-carotene was significantly degraded by UVA, but this was not accelerated by Me₂S₄ in a cell culture system. The photodegradation products of β-carotene prepared by UVA irradiation regardless of the addition of Me₂S₄ showed lower cytotoxicity than β-carotene itself in HL-60 cells. These results suggest that the ROS- and caspase-3-dependent apoptosis induced by β-carotene and Me₂S₄ assisted by UVA was due to a synergistic action rather than to the sole effect of the photodegradation products of β-carotene in HL-60 cells.

DOI： 10.1271/bbb.80794

Other Link： https://jlc.jst.go.jp/DN/JALC/00331262726?from=CiNii

De-regulated expression of the plant glutamate receptor homolog AtGLR3.1 impairs long-term Ca2+-programmed stomatal closure. International journal

Daeshik Cho, Sun A Kim, Yoshiyuki Murata, Sangmee Lee, Seul-Ki Jae, Hong Gil Nam, June M Kwak

The Plant journal : for cell and molecular biology 58 ( 3 ) 437 - 49 2009.5

Language：English Publishing type：Research paper (scientific journal)

Cytosolic Ca(2+) ([Ca(2+)](cyt)) mediates diverse cellular responses in both animal and plant cells in response to various stimuli. Calcium oscillation amplitude and frequency control gene expression. In stomatal guard cells, [Ca(2+)](cyt) has been shown to regulate stomatal movements, and a defined window of Ca(2+) oscillation kinetic parameters encodes necessary information for long-term stomatal movements. However, it remains unknown how the encrypted information in the cytosolic Ca(2+) signature is decoded to maintain stomatal closure. Here we report that the Arabidopsis glutamate receptor homolog AtGLR3.1 is preferentially expressed in guard cells compared to mesophyll cells. Furthermore, over-expression of AtGLR3.1 using a viral promoter resulted in impaired external Ca(2+)-induced stomatal closure. Cytosolic Ca(2+) activation of S-type anion channels, which play a central role in Ca(2+)-reactive stomatal closure, was normal in the AtGLR3.1 over-expressing plants. Interestingly, AtGLR3.1 over-expression did not affect Ca(2+)-induced Ca(2+) oscillation kinetics, but resulted in a failure to maintain long-term 'Ca(2+)-programmed' stomatal closure when Ca(2+) oscillations containing information for maintaining stomatal closure were imposed. By contrast, prompt short-term Ca(2+)-reactive closure was not affected in AtGLR3.1 over-expressing plants. In wild-type plants, the translational inhibitor cyclohexamide partially inhibited Ca(2+)-programmed stomatal closure induced by experimentally imposed Ca(2+) oscillations without affecting short-term Ca(2+)-reactive closure, mimicking the guard cell behavior of the AtGLR3.1 over-expressing plants. Our results suggest that over-expression of AtGLR3.1 impairs Ca(2+) oscillation-regulated stomatal movements, and that de novo protein synthesis contributes to the maintenance of long-term Ca(2+)-programmed stomatal closure.

DOI： 10.1111/j.1365-313X.2009.03789.x

(-)-Epigallocatechin-3-gallate induces up-regulation of Th1 and Th2 cytokine genes in Jurkat T cells. International journal

Haitao Wu, Beiwei Zhu, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

Archives of biochemistry and biophysics 483 ( 1 ) 99 - 105 2009.3

Language：English Publishing type：Research paper (scientific journal)

In the present study, we found that (-)-epigallocatechin-3-gallate (EGCG) significantly up-regulated the mRNA expression of the Th1/Th2 cytokines including IL-2, IFN-gamma, IL-5 and IL-13 in Jurkat T cells. The EGCG-induced mRNA up-regulation of IL-2 and IL-5 was predominantly affected by the extracellular signal-regulated protein kinase (ERK) signalling, whereas IL-13 gene expression, the most responsive to the EGCG treatment, was dependent on neither ERK nor c-jun NH(2)-terminal kinase (JNK) signalling. IFN-gamma gene expression was partially mitigated by both inhibitors of the ERK and JNK pathways. Furthermore, catalase significantly attenuated the intracellular peroxide production, phosphorylation of ERK and JNK, and all cytokine gene expressions induced by EGCG. In addition, physiologically relevant concentrations of both EGCG and H(2)O(2)-induced up-regulation of IL-5 gene expression. Our findings provide biological evidence that EGCG induces Th1/Th2 cytokine mRNA expression via H(2)O(2) production followed by activation of ERK or JNK in Jurkat T cells.

DOI： 10.1016/j.abb.2008.12.010

Nitric oxide functions in both methyl jasmonate signaling and abscisic acid signaling in Arabidopsis guard cells. International journal

Naoki Saito, Yoshimasa Nakamura, Izumi C Mori, Yoshiyuki Murata

Plant signaling & behavior 4 ( 2 ) 119 - 20 2009.2

Language：English Publishing type：Research paper (scientific journal)

Intracellular components in methyl jasmonate (MeJA) signaling remain largely unknown, to compare those in well-understood abscisic acid (ABA) signaling. We have reported that nitric oxide (NO) is a signaling component in MeJA-induced stomatal closure, as well as ABA-induced stomatal closure in the previous study. To gain further information about the role of NO in the guard cell signaling, NO production was examined in an ABA- and MeJA-insensitive Arabidopsis mutant, rcn1. Neither MeJA nor ABA induced NO production in rcn1 guard cells. Our data suggest that NO functions downstream of the branch point of MeJA and ABA signaling in Arabidopsis guard cells.

Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress. International journal

Nasrin Akhter Banu, Anamul Hoque, Megumi Watanabe-Sugimoto, Ken Matsuoka, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

Journal of plant physiology 166 ( 2 ) 146 - 56 2009.1

Language：English Publishing type：Research paper (scientific journal)

Salt stress causes oxidative damage and cell death in plants. Plants accumulate proline and glycinebetaine (betaine) to mitigate detrimental effects of salt stress. The aim of this study was to investigate the protective effects of proline and betaine on cell death in NaCl-unadapted tobacco (Nicotiana tabacum) Bright Yellow-2 suspension-cultured cells subjected to salt stress. Salt stress increased reactive oxygen species (ROS) accumulation, lipid peroxidation, nuclear deformation and degradation, chromatin condensation, apoptosis-like cell death and ATP contents. Neither proline nor betaine affected apoptosis-like cell death and G(1) phase population, and increased ATP contents in the 200mM NaCl-stressed cells. However, both of them effectively decreased ROS accumulation and lipid peroxidation, and suppressed nuclear deformation and chromatin condensation induced by severe salt stress. Evans Blue staining experiment showed that both proline and betaine significantly suppressed increment of membrane permeability induced by 200mM NaCl. Furthermore, among the ROS scavenging antioxidant defense genes studied here, mRNA levels of salicylic acid-binding (SAbind) catalase (CAT) and lignin-forming peroxidase (POX) were found to be increased by proline and betaine under salt stress. It is concluded that both proline and betaine provide a protection against NaCl-induced cell death via decreasing level of ROS accumulation and lipid peroxidation as well as improvement of membrane integrity.

DOI： 10.1016/j.jplph.2008.03.002

Docosahexaenoic acid induces ERK1/2 activation and neuritogenesis via intracellular reactive oxygen species production in human neuroblastoma SH-SY5Y cells. International journal

Haitao Wu, Sanae Ichikawa, Chiharu Tani, Beiwei Zhu, Mikiro Tada, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

Biochimica et biophysica acta 1791 ( 1 ) 8 - 16 2009.1

Language：English Publishing type：Research paper (scientific journal)

Docosahexaenoic acid (22: 6n-3; DHA) is a long chain polyunsaturated fatty acid that exists highly enriched in fish oil, and it is one of the low molecular weight food chemicals which can pass a blood brain barrier. A preliminary survey of several fatty acids for expression of growth-associated protein-43 (GAP-43), a marker of axonal growth, identified DHA as one of the most potent inducers. The human neuroblastoma SH-SY5Y cells exposed to DHA showed significant and dose-dependent increases in the percentage of cells with longer neurites. To elucidate signaling mechanisms involved in DHA-enhanced basal neuritogenesis, we examined the role of extracellular signal-regulated kinase (ERK)1/2 and intracellular reactive oxygen species (ROS) production using SH-SY5Y cells. From immunoblotting experiments, we observed that DHA induced the ROS production, protein tyrosine phosphatase inhibition, mitogen-activated protein kinase (MAPK)/ERK kinase (MEK) phosphorylation, and sequentially ERK1/2 phosphorylation, the last of which was significantly reduced by MEK inhibitor U0126. Both antioxidants and MEK inhibitor affected DHA-induced GAP-43 expression, whereas the specific PI3K inhibitor LY294002 did not. We found that total protein tyrosine phosphatase activity was also downregulated by DHA treatment, which was counteracted by antioxidant pretreatment. These results suggest that the ROS-dependent ERK pathway, rather than PI3K, plays an important role during DHA-enhanced neurite outgrowth.

DOI： 10.1016/j.bbalip.2008.10.004

Roles of RCN1, a Protein Phosphatase 2A A subunit, in Methyl Jasmonate Signaling in Arabidopsis Guard Cells

Saito Naoki, Nakamura Yoshimasa, Mori C. Izumi, Murata Yoshiyuki

Plant and Cell Physiology Supplement 2009 0451 - 0451 2009

Publisher：The Japanese Society of Plant Physiologists

In plants, metyl jasmonate (MeJA), which mediates various plant defense response, as well as abscisic acid (ABA) induces stomatal closure. It has been shown that there are various signaling factors in MeJA signaling in guard cells. RCN1, an Arabidopsis protein phosphatase 2A (PP2A) A subunit, is involved in auxin signaling in roots and ABA signaling in guard cells. Previously, our study suggested that RCN1 functions as a positive regulator in MeJA signaling in guard cells. RCN1 is required for MeJA regulation of reactive oxygen species production and inward-rectifying potassium ion channel currents in guard cells. <br> In order to gain further information about MeJA signaling in guard cells, we evaluated roles of RCN1 in MeJA regulation of nitric oxide production, calcium ion channel currents, and S-type anion channel currents in guard cells. Our results suggest a novel model of MeJA signaling in Arabidopsis guard cells.

DOI： 10.14841/jspp.2009.0.0451.0

(-)-Epigallocatechin-3-gallate Potentiates the Cytotoxicity Induced by Benzyl Isothiocyanate and Hydrogen Peroxide in Human Jurkat T Lymphocytes International journal

WU Haitao, YOKOYAMA Tomoko, ZHU Beiwei, SHIMOISHI Yasuaki, MURATA Yoshiyuki, NAKAMURA Yoshimasa

Agricultural and Biological Chemistry 72 ( 11 ) 3034 - 3037 2008.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

(−)-Epigallocatechin-3-gallate (EGCG)-induced apoptosis was along both the extracellular signal-regulated protein kinase (ERK) and c-jun N-terminal kinase (JNK) pathways in Jurkat cells. Co-treatment with EGCG potentiated the cytotoxicity induced by benzyl isothiocyanate (BITC) and H₂O₂, both being inhibited by ERK and JNK inhibitors. These results suggest the significant role of mitogen-activated protein kinase (MAPK) signaling in the apoptosis induction regulated by EGCG alone and in combination.

DOI： 10.1271/bbb.80422

Other Link： https://jlc.jst.go.jp/DN/JALC/00321431040?from=CiNii

Effect of Dimethyl Sulfides on the Induction of Apoptosis in Human Leukemia Jurkat Cells and HL-60 Cells International journal

ZHANG Gongliang, WU Haitao, ZHU Beiwei, SHIMOISHI Yasuaki, NAKAMURA Yoshimasa, MURATA Yoshiyuki

Agricultural and Biological Chemistry 72 ( 11 ) 2966 - 2972 2008.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Organosulfur compounds have been established to possess anticancer effects. To provide a better understanding of the biological function of dimethyl sulfides, dimethyl monosulfide (Me₂S), dimethyl disulfide (Me₂S₂), dimethyl trisulfide (Me₂S₃) and dimethyl tetrasulfide (Me₂S₄) were used as experimental materials to investigate their effects on apoptosis induction in human leukemia Jurkat cells and HL-60 cells. Treatment with 20 μ<small>M</small> dimethyl sulfides for 24 h decreased the viability of both cells. The cell viability-reducing effect of these sulfides was in the following order: Me₂S₄ ≈ Me₂S₃ > Me₂S₂ ≈ Me₂S for Jurkat cells and Me₂S₄ > Me₂S₃ > Me₂S₂ ≈ Me₂S for HL-60 cells. Me₂S₃ and Me₂S₄ significantly induced DNA fragmentation and caspase-3 activation. The addition of GSH or NAC completely suppressed the sulfide-induced apoptosis. Our results indicate that dimethyl sulfides with a larger number of sulfur atoms more strongly induced apoptosis in both human leukemia cells via ROS production and caspase-3 activation.

DOI： 10.1271/bbb.80453

Other Link： https://jlc.jst.go.jp/DN/JALC/00321430906?from=CiNii

Deficient Glutathione in Guard Cells Facilitates Abscisic Acid-Induced Stomatal Closure but Does Not Affect Light-Induced Stomatal Opening International journal

JAHAN Md. Sarwar, OGAWA Ken'ichi, NAKAMURA Yoshimasa, SHIMOISHI Yasuaki, MORI Izumi C., MURATA Yoshiyuki

Agricultural and Biological Chemistry 72 ( 10 ) 2795 - 2798 2008.10

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

We investigated the role of glutathione (GSH) in stomatal movements using a GSH deficient mutant, chlorinal-1 (ch1-1). Guard cells of ch1-1 mutants accumulated less GSH than wild types did. Light induced stomatal opening in ch1-1 and wild-type plants. Abscisic acid (ABA) induced stomatal closure in ch1-1 mutants more than wild types without enhanced reactive oxygen species (ROS) production. Therefore, GSH functioned downstream of ROS production in the ABA signaling cascade.

DOI： 10.1271/bbb.80407

Other Link： https://jlc.jst.go.jp/DN/JALC/00319864676?from=CiNii

ATP depletion alters the mode of cell death induced by benzyl isothiocyanate Reviewed International journal

Noriyuki Miyoshi, Etsuko Watanabe, Toshihiko Osawa, Masashi Okuhira, Yoshiyuki Murata, Hiroshi Ohshima, Yoshimasa Nakamura

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1782 ( 10 ) 566 - 573 2008.10

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Pro-inflammatory death is presumably an undesirable event in cancer prevention process, thus biochemical comprehension and molecular definition of this process could have important clinical implications. In the present study, we examined the cytophysiological conversion of cell death mode by benzyl isothiocyanate (BITC) in human cervical cancer HeLa cells. The detailed studies using flow cytometric and morphological analyses demonstrated that the cells treated with appropriate concentration (25 mu M) of BITC showed apoptotic feature, such as chromatin condensation, DNA fragmentation, and preserved plasma membrane integrity, whereas these features were disappeared by treatment with higher concentration (100 mu M). The treatment with 2-deoxyglucose, an inhibitor of ATP synthesis, drastically increased in the ratio of necrotic dead cells, while it influences little that of apoptotic cells. Moreover, an analysis using the mitochondrial DNA-deficient HeLa cells demonstrated that the rho degrees cells were more susceptible to the BITC-induced necrosis-like cell death compared to the wild-type (rho(+)) cells, whereas the ROS production was significantly inhibited in the rho degrees cells. It is likely that the BITC-induced ROS is derived from mitochondrial respiratory chain and ruled out the contribution to the mechanism of cell death mode switching. In addition, the BITC treatment resulted in a more rapid depletion of ATP in the rho degrees cells than in the rho(+) cells. Furthermore. a caspase inhibitor, Z-VAD-fmk counteracted not only apoptosis, but also necrosis-like cell death induced by BITC, suggesting that increment in this cell death pattern might be due to the interruption of events downstream of a caspase-dependent pathway. The obtained data suggested that the decline in the intracellular ATP level plays an important role in tuning the mode of cell death by BITC. (C) 2008 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.bbadis.2008.07.002

Roles of RCN1, Regulatory A Subunit of Protein Phosphatase 2A, in Methyl Jasmonate Signaling and Signal Crosstalk between Methyl Jasmonate and Abscisic Acid

SAITO Naoki, MUNEMASA Shintaro, NAKAMURA Yoshimasa, SHIMOISHI Yasuaki, MORI Izumi C., MURATA Yoshiyuki

Plant and Cell Physiology 49 ( 9 ) 1396 - 1401 2008.9

Language：English Publishing type：Research paper (scientific journal)

Methyl jasmonate (MeJA) as well as abscisic acid (ABA) induces stomatal closure with their signal crosstalk. We investigated the function of a regulatory A subunit of protein phosphatase 2A, RCN1, in MeJA signaling. Both MeJA and ABA failed to induce stomatal closure in Arabidopsis rcn1 knockout mutants unlike in wild-type plants. Neither MeJA nor ABA induced reactive oxygen species (ROS) production and suppressed inward-rectifying potassium channel activities in rcn1 mutants but not in wild-type plants. These results suggest that RCN1 functions upstream of ROS production and downstream of the branch point of MeJA signaling and ABA signaling in Arabidopsis guard cells.

DOI： 10.1093/pcp/pcn106

Purification of the functional plant membrane channel KAT1 Reviewed International journal

Takao Hibi, Shiho Aoki, Keisuke Oda, Shintaro Munemasa, Shunsuke Ozaki, Osamu Shirai, Yoshiyuki Murata, Nobuyuki Uozumi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 374 ( 3 ) 465 - 469 2008.9

Language：English Publishing type：Research paper (scientific journal) Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE

The inward-rectifying K+ channel KAT1 is expressed mainly in Arabidopsis thaliana guard cells. The Purification of functional KAT1 has never been reported. We investigated the extraction of the plant K+ channel KAT1 with different detergents, as an example for how to select detergents for purifying a eukaryotic membrane protein. A KAT1-GFP fusion protein was used to screen a library of 46 detergents for the effective solubilization of intact KAT1. Then, a "test set" of three detergents was picked for further analysis, based on their biochemical characteristics and availability. The combination use of the selected detergents enabled the effective purification Of functional KAT1 with affinity and gel-filtration chromatography. (C) 2008 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2008.07.026

Structure-Dependent Photodegradation of Carotenoids Accelerated by Dimethyl Tetrasulfide under UVA Irradiation International journal

ZHANG Gongliang, ZHU Beiwei, NAKAMURA Yoshimasa, SHIMOISHI Yasuaki, MURATA Yoshiyuki

Agricultural and Biological Chemistry 72 ( 8 ) 2176 - 2183 2008.8

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Carotenoids are used in wide-ranging food applications, but they are susceptible to degradation by many factors including light. We examined the photodegradation of five kinds of carotenoids and three kinds of anthocyanins to clarify which structures of pigments were favorable to accelerated degradation by sulfides under UVA irradiation. Under UVA irradiation, crocetin and crocin were decomposed more rapidly in the presence of dimethyl tetrasulfide than in the absence of the sulfide, but not as rapidly as β-carotene, zeaxanthin and β-cryptoxanthin were. However, cyanidin was decomposed more slowly in the presence of sulfide than in the absence of sulfide. Moreover, the photodegradation of kuromanin and keracyanin was not affected by the addition of a sulfide. We also examined the mechanism for this accelerated degradation. Normal hexane was more favorable to the photodegradation of β-carotene than methanol and ethanol. The accelerated degradation was inhibited by free radical scavengers, but enhanced by the addition of deuterium oxide. These results suggest that conjugated double bonds were favorable to the accelerated photodegradation by sulfide and that this reaction was mediated by free radicals.

DOI： 10.1271/bbb.80251

Other Link： https://jlc.jst.go.jp/DN/JALC/00317059166?from=CiNii

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