Faculty Profiles - MURATA Yoshiyuki

Papers - MURATA Yoshiyuki

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Disarming the jasmonate-dependent plant defense makes nonhost Arabidopsis plants accessible to the American serpentine leafminer. International journal

Hiroshi Abe, Ken Tateishi, Shigemi Seo, Soichi Kugimiya, Masami Yokota Hirai, Yuji Sawada, Yoshiyuki Murata, Kaori Yara, Takeshi Shimoda, Masatomo Kobayashi

Plant physiology 163 ( 3 ) 1242 - 53 2013.11

Language：English Publishing type：Research paper (scientific journal)

Here, we analyzed the interaction between Arabidopsis (Arabidopsis thaliana) and the American serpentine leafminer (Liriomyza trifolii), an important and intractable herbivore of many cultivated plants. We examined the role of the immunity-related plant hormone jasmonate (JA) in the plant response and resistance to leafminer feeding to determine whether JA affects host suitability for leafminers. The expression of marker genes for the JA-dependent plant defense was induced by leafminer feeding on Arabidopsis wild-type plants. Analyses of JA-insensitive coi1-1 mutants suggested the importance of JA in the plant response to leafminer feeding. The JA content of wild-type plants significantly increased after leafminer feeding. Moreover, coi1-1 mutants showed lower feeding resistance against leafminer attack than did wild-type plants. The number of feeding scars caused by inoculated adult leafminers in JA-insensitive coi1-1 mutants was higher than that in wild-type plants. In addition, adults of the following generation appeared only from coi1-1 mutants and not from wild-type plants, suggesting that the loss of the JA-dependent plant defense converted nonhost plants to accessible host plants. Interestingly, the glucosinolate-myrosinase defense system may play at most a minor role in this conversion, indicating that this major antiherbivore defense of Brassica species plants probably does not have a major function in plant resistance to leafminer. Application of JA to wild-type plants before leafminer feeding enhanced feeding resistance in Chinese cabbage (Brassica rapa), tomato (Solanum lycopersicum), and garland chrysanthemum (Chrysanthemum coronarium). Our results indicate that JA plays an important role in the plant response and resistance to leafminers and, in so doing, affects host plant suitability for leafminers.

DOI： 10.1104/pp.113.222802

Calcium-Dependent Protein Kinase CPK6 Positively Functions in Induction by Yeast Elicitor of Stomatal Closure and Inhibition by Yeast Elicitor of Light-Induced Stomatal Opening in Arabidopsis International journal

Wenxiu Ye, Daichi Muroyama, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

PLANT PHYSIOLOGY 163 ( 2 ) 591 - 599 2013.10

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Yeast elicitor (YEL) induces stomatal closure that is mediated by a Ca2+-dependent signaling pathway. A Ca2+-dependent protein kinase, CPK6, positively regulates activation of ion channels in abscisic acid and methyl jasmonate signaling, leading to stomatal closure in Arabidopsis (Arabidopsis thaliana). YEL also inhibits light-induced stomatal opening. However, it remains unknown whether CPK6 is involved in induction by YEL of stomatal closure or in inhibition by YEL of light-induced stomatal opening. In this study, we investigated the roles of CPK6 in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis. Disruption of CPK6 gene impaired induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening. Activation by YEL of nonselective Ca2+-permeable cation channels was impaired in cpk6-2 guard cells, and transient elevations elicited by YEL in cytosolic-free Ca2+ concentration were suppressed in cpk6-2 and cpk6-1 guard cells. YEL activated slow anion channels in wild-type guard cells but not in cpk6-2 or cpk6-1 and inhibited inward-rectifying K+ channels in wild-type guard cells but not in cpk6-2 or cpk6-1. The cpk6-2 and cpk6-1 mutations inhibited YEL-induced hydrogen peroxide accumulation in guard cells and apoplast of rosette leaves but did not affect YEL-induced hydrogen peroxide production in the apoplast of rosette leaves. These results suggest that CPK6 positively functions in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis and is a convergent point of signaling pathways for stomatal closure in response to abiotic and biotic stress.

DOI： 10.1104/pp.113.224055

Difference in abscisic acid perception mechanisms between closure induction and opening inhibition of stomata. International journal

Ye Yin, Yuji Adachi, Wenxiu Ye, Maki Hayashi, Yoshimasa Nakamura, Toshinori Kinoshita, Izumi C Mori, Yoshiyuki Murata

Plant physiology 163 ( 2 ) 600 - 10 2013.10

Language：English Publishing type：Research paper (scientific journal)

Abscisic acid (ABA) induces stomatal closure and inhibits light-induced stomatal opening. The mechanisms in these two processes are not necessarily the same. It has been postulated that the ABA receptors involved in opening inhibition are different from those involved in closure induction. Here, we provide evidence that four recently identified ABA receptors (PYRABACTIN RESISTANCE1 [PYR1], PYRABACTIN RESISTANCE-LIKE1 [PYL1], PYL2, and PYL4) are not sufficient for opening inhibition in Arabidopsis (Arabidopsis thaliana). ABA-induced stomatal closure was impaired in the pyr1/pyl1/pyl2/pyl4 quadruple ABA receptor mutant. ABA inhibition of the opening of the mutant's stomata remained intact. ABA did not induce either the production of reactive oxygen species and nitric oxide or the alkalization of the cytosol in the quadruple mutant, in accordance with the closure phenotype. Whole cell patch-clamp analysis of inward-rectifying K(+) current in guard cells showed a partial inhibition by ABA, indicating that the ABA sensitivity of the mutant was not fully impaired. ABA substantially inhibited blue light-induced phosphorylation of H(+)-ATPase in guard cells in both the mutant and the wild type. On the other hand, in a knockout mutant of the SNF1-related protein kinase, srk2e, stomatal opening and closure, reactive oxygen species and nitric oxide production, cytosolic alkalization, inward-rectifying K(+) current inactivation, and H(+)-ATPase phosphorylation were not sensitive to ABA.

DOI： 10.1104/pp.113.223826

Endogenous abscisic acid is involved in methyl jasmonate-induced reactive oxygen species and nitric oxide production but not in cytosolic alkalization in Arabidopsis guard cells International journal

Wenxiu Ye, Mohammad Anowar Hossain, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Journal of Plant Physiology 170 ( 13 ) 1212 - 1215 2013.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

We recently demonstrated that endogenous abscisic acid (ABA) is involved in methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we investigated whether endogenous ABA is involved in MeJA-induced reactive oxygen species (ROS) and nitric oxide (NO) production and cytosolic alkalization in guard cells using an ABA-deficient Arabidopsis mutant, aba2-2, and an inhibitor of ABA biosynthesis, fluridon (FLU). The aba2-2 mutation impaired MeJA-induced ROS and NO production. FLU inhibited MeJA-induced ROS production in wild-type guard cells. Pretreatment with 0.1 μM ABA, which does not induce stomatal closure in the wild type, complemented the insensitivity to MeJA of the aba2-2 mutant. However, MeJA induced cytosolic alkalization in both wild-type and aba2-2 guard cells. These results suggest that endogenous ABA is involved in MeJA-induced ROS and NO production but not in MeJA-induced cytosolic alkalization in Arabidopsis guard cells.

DOI： 10.1016/j.jplph.2013.03.011

Effects of krill oil intake on plasma cholesterol and glucose levels in rats fed a high-cholesterol diet. International journal

Dong-Mei Li, Da-Yong Zhou, Bei-Wei Zhu, Ya-Li Chi, Li-Ming Sun, Xiu-Ping Dong, Lei Qin, Wei-Zhou Qiao, Yoshiyuki Murata

Journal of the science of food and agriculture 93 ( 11 ) 2669 - 75 2013.8

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: In this study, whole krill oil (WKO) and phospholipid-type krill oil (PKO) with different lipid composition were prepared. The effects of KO intake on plasma cholesterol and glucose levels in Wistar rats fed a high-cholesterol diet (HCD) were investigated. RESULTS: WKO contained 37.63% triglycerides, 48.37% phospholipids, 13.54% free fatty acids and 0.66% cholesterol, whereas the corresponding values for PKO were 0.59, 69.80, 28.53 and 1.09% respectively. Meanwhile, PKO contained much more polyunsaturated fatty acids (PUFA, 37.76%) than WKO (28.36%). After 4 weeks of HCD consumption, plasma levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and glucose increased significantly, but that of high-density lipoprotein cholesterol (HDL-C) decreased significantly. The intake of PKO and WKO for 4 weeks caused a significant reduction in body weight gain and plasma levels of TC and LDL-C in HCD-fed rats. Compared with WKO, PKO was more effective in decreasing plasma TC and LDL-C levels. CONCLUSION: PKO showed better overall cholesterol-lowering effects than WKO, which may be due to its higher n-3 PUFA levels.

DOI： 10.1002/jsfa.6072

bHLH transcription factors that facilitate K⁺ uptake during stomatal opening are repressed by abscisic acid through phosphorylation. International journal

Yohei Takahashi, Yuta Ebisu, Toshinori Kinoshita, Michio Doi, Eiji Okuma, Yoshiyuki Murata, Ken-Ichiro Shimazaki

Science signaling 6 ( 280 ) ra48 2013.6

Language：English Publishing type：Research paper (scientific journal)

Stomata open in response to light and close after exposure to abscisic acid (ABA). They regulate gas exchange between plants and the atmosphere, enabling plants to adapt to changing environmental conditions. ABA binding to receptors initiates a signaling cascade that involves protein phosphorylation. We show that ABA induced the phosphorylation of three basic helix-loop-helix (bHLH) transcription factors, called AKSs (ABA-responsive kinase substrates; AKS1, AKS2, and AKS3), in Arabidopsis guard cells. In their unphosphorylated state, AKSs facilitated stomatal opening through the transcription of genes encoding inwardly rectifying K⁺ channels. aks1aks2-1 double mutant plants showed decreases in light-induced stomatal opening, K⁺ accumulation in response to light, activity of inwardly rectifying K⁺ channels, and transcription of genes encoding major inwardly rectifying K⁺ channels without affecting ABA-mediated stomatal closure. Overexpression of potassium channel in Arabidopsis thaliana 1 (KAT1), which encodes a major inwardly rectifying K⁺ channel in guard cells, rescued the phenotype of aks1aks2-1 plants. AKS1 bound directly to the promoter of KAT1, an interaction that was attenuated after ABA-induced phosphorylation. The ABA agonist pyrabactin induced phosphorylation of AKSs. Our results demonstrate that the AKS family of bHLH transcription factors facilitates stomatal opening through the transcription of genes encoding inwardly rectifying K⁺ channels and that ABA suppresses the activity of these channels by triggering the phosphorylation of AKS family transcription factors.

DOI： 10.1126/scisignal.2003760

Neither Endogenous Abscisic Acid nor Endogenous Jasmonate Is Involved in Salicylic Acid-, Yeast Elicitor-, or Chitosan-Induced Stomatal Closure in Arabidopsis thaliana International journal

ISSAK Mohammad, OKUMA Eiji, MUNEMASA Shintaro, NAKAMURA Yoshimasa, MORI Izumi C., MURATA Yoshiyuki

Agricultural and Biological Chemistry 77 ( 5 ) 1111 - 1113 2013.5

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Salicylic acid (SA), yeast elicitor (YEL), and chitosan (CHT) induced stomatal closure in Arabidopsis wild-type and aba2-2 plants, induced stomatal closure in fluridon-treated wild-type plants, and induced stomatal closure in aos mutants. These results suggest that neither endogenous abscisic acid nor endogenous jasmonic acid is involved in SA-, YEL-, or CHT-induced stomatal closure.

DOI： 10.1271/bbb.120980

Other Link： https://jlc.jst.go.jp/DN/JALC/10019154052?from=CiNii

Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells Reviewed

M. A. Salam, F. Jammes, M. A. Hossain, W. Ye, Y. Nakamura, I. C. Mori, J. M. Kwak, Y. Murata

Plant Biology 15 ( 3 ) 436 - 442 2013.5

Language：English Publishing type：Research paper (scientific journal)

We report that two mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate abscisic acid (ABA)-induced stomatal closure in Arabidopsis thaliana. Yeast elicitor (YEL) induced stomatal closure accompanied by intracellular reactive oxygen species (ROS) accumulation and cytosolic free calcium concentration ([Ca2+]cyt) oscillation. In this study, we examined whether these two MAP kinases are involved in YEL-induced stomatal closure using MAPKK inhibitors, PD98059 and U0126, and MAPK mutants, mpk9, mpk12 and mpk9 mpk12. Both PD98059 and U0126 inhibited YEL-induced stomatal closure. YEL induced stomatal closure in the mpk9 and mpk12 mutants but not in the mpk9 mpk12 mutant, suggesting that a MAPK cascade involving MPK9 and MPK12 functions in guard cell YEL signalling. However, YEL induced extracellular ROS production, intracellular ROS accumulation and cytosolic alkalisation in the mpk9, mpk12 and mpk9 mpk12 mutants. YEL induced [Ca2+]cyt oscillations in both wild type and mpk9 mpk12 mutant. These results suggest that MPK9 and MPK12 function redundantly downstream of extracellular ROS production, intracellular ROS accumulation, cytosolic alkalisation and [Ca2+]cyt oscillation in YEL-induced stomatal closure in Arabidopsis guard cells and are shared with ABA signalling. © 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.

DOI： 10.1111/j.1438-8677.2012.00671.x

Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis

Nasima Akter, Eiji Okuma, Muhammad Abdus Sobahan, Misugi Uraji, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Journal of Plant Growth Regulation 32 ( 1 ) 208 - 215 2013.3

SIZ1 deficiency causes reduced stomatal aperture and enhanced drought tolerance via controlling salicylic acid-induced accumulation of reactive oxygen species in Arabidopsis International journal

Kenji Miura, Hiroyuki Okamoto, Eiji Okuma, Hayato Shiba, Hiroshi Kamada, Paul M. Hasegawa, Yoshiyuki Murata

Plant Journal 73 ( 1 ) 91 - 104 2013.1

Language：English Publishing type：Research paper (scientific journal)

Transpiration and gas exchange occur through stomata. Thus, the control of stomatal aperture is important for the efficiency and regulation of water use, and for the response to drought. Here, we demonstrate that SIZ1-mediated endogenous salicylic acid (SA) accumulation plays an important role in stomatal closure and drought tolerance. siz1 reduced stomatal apertures. The reduced stomatal apertures of siz1 were inhibited by the application of peroxidase inhibitors, salicylhydroxamic acid and azide, which inhibits SA-dependent reactive oxygen species (ROS) production, but not by an NADPH oxidase inhibitor, diphenyl iodonium chloride, which inhibits ABA-dependent ROS production. Furthermore, the introduction of nahG into siz1, which reduces SA accumulation, restored stomatal opening. Stomatal closure is generally induced by water deficit. The siz1 mutation caused drought tolerance, whereas nahG siz1 suppressed the tolerant phenotype. Drought stresses also induced expression of SA-responsive genes, such as PR1 and PR2. Furthermore, other SA-accumulating mutants, cpr5 and acd6, exhibited stomatal closure and drought tolerance, and nahG suppressed the phenotype of cpr5 and acd6, as did siz1 and nahG siz1. Together, these results suggest that SIZ1 negatively affects stomatal closure and drought tolerance through the accumulation of SA. © 2012 The Authors.

DOI： 10.1111/tpj.12014

Catalase, CAT2, is not Involved in Mitigation of Gamma Irradiation-induced H₂O₂ Accumulation or Lipid Peroxidation in Arabidopsis thaliana

Sultana Amena, Minami Ikuko, Matsushima Daiki, Issak Mohammad, Nakamura Yoshimasa, Todoriki Setsuko, Murata Yoshiyuki

FOOD IRRADIATION, JAPAN 48 ( 1 ) 38 - 41 2013

Language：Japanese Publisher：JAPANESE RESEARCH ASSOCIATION FOR FOOD IRRADIATION

In Arabidopsis wild-type (Col-0 ecotype) and cat2 mutant plants, gamma radiation induced H₂O₂ accumulation and lipid peroxidation at 10 kGy but not at 0.1 to 1 kGy. There were no significant differences in H₂O₂ accumulation and lipid peroxidation between the wild type and the cat2 mutant. Hence, CAT2 may not be a key enzyme to protect gamma irradiation-induced damage.

DOI： 10.5986/jrafi.48.38

Other Link： http://agriknowledge.affrc.go.jp/RN/2010870113

Effects of Emulsifiers on the Photostability of Lycopene

NISHINO Masayuki, SAKATA Makoto, MURATA Yoshiyuki, NAKAMURA Yoshimasa

Food Science and Technology International, Tokyo 19 ( 6 ) 983 - 987 2013

Language：English Publisher：Japanese Society for Food Science and Technology

Aqueous solutions of dispersed lycopene in which gum arabic, gum ghatti and polyglycerol monostearate ester had been used as emulsifiers were photoirradiated. The photodegradation rate of lycopene tended to vary depending on the amount of polyglycerol monostearate ester used. We found that the photodegradation rate of lycopene with polyglycerol monostearate ester was considerably slower than those with gum arabic and gum ghatti. Therefore, this study suggests that the absorption state of the emulsifier on the crystal surface may affect the photodegradation rate of lycopene.

DOI： 10.3136/fstr.19.983

Regulation of reactive oxygen species-mediated abscisic acid signaling in guard cells and drought tolerance by glutathione International journal

Shintaro Munemasa, Daichi Muroyama, Hiroki Nagahashi, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Frontiers in Plant Science 4 472 - 472 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers Media SA

The phytohormone abscisic acid (ABA) induces stomatal closure in response to drought stress, leading to reduction of transpirational water loss. A thiol tripeptide glutathione (GSH) is an important regulator of cellular redox homeostasis in plants. Although it has been shown that cellular redox state of guard cells controls ABA-mediated stomatal closure, roles of GSH in guard cell ABA signaling were largely unknown. Recently we demonstrated that GSH functions as a negative regulator of ABA signaling in guard cells. In this study we performed more detailed analyses to reveal how GSH regulates guard cell ABA signaling using the GSH-deficient Arabidopsis mutant cad2-1. The cad2-1 mutant exhibited reduced water loss from rosette leaves. Whole-cell current recording using patch clamp technique revealed that the cad2-1 mutation did not affect ABA regulation of S-type anion channels. We found enhanced activation of Ca(2+) permeable channels by hydrogen peroxide (H2O2) in cad2-1 guard cells. The cad2-1 mutant showed enhanced H2O2-induced stomatal closure and significant increase of ROS accumulation in whole leaves in response to ABA. Our findings provide a new understanding of guard cell ABA signaling and a new strategy to improve plant drought tolerance.

DOI： 10.3389/fpls.2013.00472

Effects of γ-Irradiation on Larval and Adult Stages of Tribolium castaneum (Red Flour Beetle) Reviewed

Sultana Amena, Minami Ikuko, Ichiba Ryuji, Issak Mohammad, Tada Mikiro, Nakamura Yoshimasa, Miyatake Takahisa, Todoriki Setsuko, Murata Yoshiyuki

FOOD IRRADIATION, JAPAN 48 ( 1 ) 19 - 23 2013

Language：Japanese Publisher：日本食品照射研究協議会

Food irradiation using γ-rays is one of powerful tools to reduce insect infestation in crops and spices. We examined the effects of γ-irradiation on larvae and adults of <i>Tribolium castaneum</i>. Eclosion for larvae was completely inhibited by irradiation at 500 Gy. A survival rate for adults was markedly reduced by irradiation at more than 500 Gy. The neutral comet assay presented that irradiation irreversibly increased DNA damage at 500 and 1000 Gy and transiently increased DNA damage at 100 Gy, suggesting possible DNA repair in larvae and adults stages. These results indicate that γ-irradiation at 500 Gy is available to eradicate the beetle at larval and adults stages.

DOI： 10.5986/jrafi.48.19

Catalases CAT1 and CAT3 are not key enzymes in alleviating gamma irradiation-induced DNA damage, H2O2 accumulation, or lipid peroxidation in Arabidopsis thaliana. International journal

Amena Sultana, Ikuko Minami, Daiki Matsushima, Mohammad Issak, Yoshimasa Nakamura, Setsuko Todoriki, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 77 ( 9 ) 1984 - 7 2013

Language：English Publishing type：Research paper (scientific journal)

Gamma irradiation increased catalase activities at 0.1 kGy and decreased them at 10 kGy in Arabidopsis wild type and catalase-deficient mutants, cat3-1 and cat1 cat3. Irradiation induced DNA damage, H2O2 accumulation, and lipid peroxidation in both mutants as well as the wild type. Thus catalases might not be key enzymes protecting gamma irradiation-induced damage.

Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in Arabidopsis thaliana. International journal

Mohammad Shakhawat Hossain, Wenxiu Ye, Mohammad Anowar Hossain, Eiji Okuma, Misugi Uraji, Yoshimasa Nakamura, Izumi C Mori, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 77 ( 5 ) 977 - 83 2013

Language：English Publishing type：Research paper (scientific journal)

Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca(2+)]cyt) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca(2+)]cyt oscillation in Arabidopsis.

Lower photostability of capsanthin dispersed in an aqueous solution. International journal

Masayuki Nishino, Makoto Sakata, Yoshiyuki Murata, Yoshimasa Nakamura

Bioscience, biotechnology, and biochemistry 77 ( 6 ) 1313 - 6 2013

Language：English Publishing type：Research paper (scientific journal)

Food-additive grades of capsanthin, lutein, lycopene, and β-carotene dispersed in aqueous solutions were photo-irradiated using a Xenon weather meter, and the levels of carotenoids were measured by HPLC and the absorbance method. Capsanthin photo-degraded more rapidly than the carotenoids tested, with less oxygen consumption. Unlike carotenes, capsanthin was partially converted into analogous colored compounds during degradation.

Effects of Depletion of Glutathione on Abscisic Acid- and Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis thaliana International journal

AKTER Nasima, SOBAHAN Muhammad Abdus, URAJI Misugi, YE Wenxiu, HOSSAIN Mohammad Anowar, MORI Izumi C., NAKAMURA Yoshimasa, MURATA Yoshiyuki

Bioscience, Biotechnology, and Biochemistry 76 ( 11 ) 2032 - 2037 2012.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Glutathione (GSH) is involved in abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJA-induced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca(2+) oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca(2+) oscillation in guard cells of Arabidopsis.

MAP Kinases, MPK9 and MPK12, Regulate Chitosan-Induced Stomatal Closure International journal

SALAM Mohammad Abdus, JAMMES Fabien, HOSSAIN Mohammad Anowar, YE Wenxiu, NAKAMURA Yoshimasa, MORI Izumi C., KWAK June M., MURATA Yoshiyuki

Bioscience, Biotechnology, and Biochemistry 76 ( 9 ) 1785 - 1787 2012.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry

Chitosan (CHT)-induced stomatal closure was inhibited by an MAPKK inhibitor, PD98059, and was impaired in mpk9 mpk12 but not in mpk9 or mpk12. CHT induced the production of reactive oxygen species, cytosolic alkalization, and cytosolic Ca(2+) oscillation in mpk9 mpk12. These results suggest that MPK9 and MPK12 are involved in CHT-induced stomatal closure.

Other Link： http://search.jamas.or.jp/link/ui/2013124407

Methylglyoxal inhibits seed germination and root elongation and up-regulates transcription of stress-responsive genes in ABA-dependent pathway in Arabidopsis Reviewed

T. S. Hoque, M. Uraji, A. Tuya, Y. Nakamura, Y. Murata

PLANT BIOLOGY 14 ( 5 ) 854 - 858 2012.9

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

Methylglyoxal (MG) is a highly reactive metabolite derived from glycolysis. In this study, we examined the effect of MG on seed germination, root elongation, chlorosis and stress-responsive gene expression in Arabidopsis using an abscisic acid (ABA)-deficient mutant, aba2-2. In the wild type, 0.1 mm MG did not affect germination but delayed root elongation, whereas 1.0 mm MG inhibited germination and root elongation and induced chlorosis. MG increased transcription levels of RD29B and RAB18 in a dose-dependent manner but did not affect RD29A transcription level. In contrast, in the aba2-2 mutant, MG inhibition of seed germination at 1.0 mm and 10.0 mm and a delay of root elongation at 0.1 mm MG were mitigated, although there was no significant difference in chlorosis between the wild type and mutant. Moreover, the aba2-2 mutation impaired MG-induced RD29B and RAB18 gene expression. These observations suggest that MG not only directly inhibits germination and root elongation but also indirectly modulates these processes via endogenous ABA in Arabidopsis.

DOI： 10.1111/j.1438-8677.2012.00607.x

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