研究者詳細 - 成瀬　恵治

論文 - 成瀬　恵治

分割表示 177 件中 101 - 120 件目／全件表示 >>

Automatic measurement system for biological applications based on pH using ISFET sensor probe 査読

Akira Yamada, Satoshi Mohri, Michihiro Nakamura, Keiji Naruse

2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010 19 - 24 2010年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）

Throughput in the cell response analysis is expected to progress in medicine and biology. In this paper, we construct a method for the measurement of cell activity that is measured by the pH fluctuation of the solution around the cell. To improve throughput of the measurements, first we constructed an automatic pH measurement system using ion-sensitive field-effect transistor (ISFET), and the system composed of 96-well microplate, pump, XYZ stage, and PC. The basic data of the cell activity was taken from human embryonic kidney 293 (HEK 293) cells by the use of flow-through type measuring chamber. ©2010 IEEE.

DOI： 10.1109/MHS.2010.5669583

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Application of mechanical stimuli using a microfluidic air actuating system to cultured mammalian embryos 査読

Jing-Chun Li, Koji Matsuura, Yuka Kuroda, Hiroaki Funahashi, Keiji Naruse

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）

Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression, and friction force, in the fallopian tube before nidation. We aim to develop a novel and simple system to apply mechanical stimuli (MS) similar to those generated inside the oviduct to cultured mammalian embryos. Possible MS include shear stress (SS) caused by fluid dynamics and compression of embryos due to interactions with the wall of the oviduct. A new culture system was developed to increase SS and to apply MS during in vitro embryo cultures. We developed an air actuating system with microfluidic channels to apply MS by deforming a 0.1-mm-thick poly(dimethylsiloxiane) membrane and evaluated MS applied to ICR mouse embryos inside the microfluidic channel. Using this air actuating system, we applied compression to mouse embryos inside the medium channel and estimated SS on the basis of the velocity of the embryos' motion. By changing the syringe velocity, we applied different types of MS to the em bryos. These results suggested that multiple MS such as SS and compression can be applied at the same time. MS applied using this system was similar to those generated in the physiological environment of the oviduct. ©2010 IEEE.

DOI： 10.1109/MHS.2010.5669581

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Optimizing the conditions for pH measurement with an automated pH measurement system using a flow-through-type differential sensor probe consisting of pH-FETs. 査読

Yamada A, Mohri S, Nakamura M, Naruse K

Journal of Robotics and Mechatronics 22 ( 2 ) 197 - 203 2010年

A rapid microfluidic perfusion system for analysis at the single cellular level 査読

Yamada A, Katanosaka Y, Mohri S, Naruse K

IEEE TRANS NanoBioscience 8 ( 4 ) 306 - 311 2009年12月

Mechanical stretch enhances IL-8 production in pulmonary microvascular endothelial cells 査読

Iwaki M, Ito S, Morioka M, Iwata S, Numaguchi Y, Ishii M, Kondo M, Kume H, Naruse K, Sokabe M, Hasegawa Y

Biochem Biophys Res Commun 389 ( 3 ) 531 - 536 2009年11月

Mesenchymal stem cell-based gene therapy with prostacyclin synthase enhanced neovascularization in hindlimb ischemia. 査読

Ishii M, Numaguchi Y, Okumura K, Kubota R, Ma X, Murakami R, Naruse K, Murohara T

Atherosclerosis 206 ( 1 ) 109 - 118 2009年9月

Mechanical stretch stimulates integrin alphaVbeta3-mediated collagen expression in human anterior cruciate ligament cells. 査読

Tetsunaga T, Furumatsu T, Abe N, Nishida K, Naruse K, Ozaki T

J Biomech 42 ( 13 ) 2097 - 2103 2009年9月

Stress-Axis Regulated Exon (STREX) in the C terminus of BK(Ca) channels is responsible for the stretch sensitivity. 査読

Naruse K, Tang QY, Sokabe M

Biochem Biophys Res Commun 385 ( 4 ) 634 - 639 2009年8月

The TRPV4 cation channel mediates stretch-evoked Ca2+ influx and ATP release in primary urothelial cell cultures. 査読

Mochizuki T, Sokabe T, Araki I, Fujishita K, Shibasaki K, Uchida K, Naruse K, Koizumi S, Takeda M, Tominaga M

J Biol Chem 284 ( 32 ) 21257 - 21264 2009年8月

Application of a numerical simulation to improve the separation efficiency of a sperm sorter 査読

Hyakutake T, Hashimoto Y, Yanase S, Matsuura K, Naruse K

Biomed Microdevices 11 ( 1 ) 25 - 33 2009年2月

Uniaxial cyclic stretch increases glucose uptake into C2C12 myotubes through a signaling pathway independent of insulin-like growth factor I. 査読

Iwata M, Suzuki S, Hayakawa K, Inoue T and Naruse K

Horm Metab Res 41 ( 1 ) 16 - 22 2009年1月

Fabricating small-scale, curved, polymeric structures with convex and concave menisci through interfacial free energy equilibrium 査読

Cheng CM, Matsuura K, Wang IJ, Kuroda Y, Leduc PR, Naruse K

Lab Chip 9 ( 22 ) 3306 - 3309 2009年

Impairment of leukocyte deformability in patients undergoing esophagectomy 査読

Suemori T, Morimatsu H, Mizobuchi S, Morita K, Katanosaka Y, Mohri S, Naruse K

Clin Hemorheol Microcirc 41 ( 2 ) 127 - 136 2009年

Application of a flow-through type pH/CO2 sensor system based on ISFET for evaluation of the glucose dependency of the metabolic pathways in cultured cells 査読

Satoshi Mohri, Akira Yamada, Noriko Goda, Michihiro Nakamura, Keiji Naruse, Fumihiko Kajiya

SENSORS AND ACTUATORS B-CHEMICAL 134 ( 2 ) 447 - 450 2008年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE SA

We have previously reported that a flow-through type pH/CO2 sensor system using two ion sensitive field effect transistors (ISFETs) could be utilized to evaluate the production rate of total carbonate (secreted CO2 + secreted bicarbonate ion) and free lactic acid (secreted lactic acid -secreted bicarbonate ion). To validate the usefulness of this system, we applied it to the quantitative analysis of metabolic switching by the change Of glucose concentrations in cultured bovine arterial endothelium cell, human umbilical vein endothelium cell and rat cardiac muscle cell. In all of these cell species, a decrease of glucose concentration increased total carbonate that represents the cellular respiration activity and decreased free lactic acid that represents glycolysis activity. We firstly analyzed a switching of metabolic pathways from glycolysis to respiration with a decrease of glucose quantitatively. This ISFET system can be applicable to many biological investigations by analyzing metabolic activity and give dynamic cellular information. (C) 2008 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.snb.2008.05.021

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Examination of signalling pathways involved in muscarinic responses in bovine ciliary muscle using YM-254890, an inhibitor of the Gq/11 protein. 査読

Yasui F, Miyazu M, Yoshida A, *Naruse K and Takai A

Br J Pharmacol 154 ( 4 ) 890 - 900 2008年6月

A novel Ca2+ influx pathway activated by mechanical stretch in human airway smooth muscle cells. 査読

Ito S, Kume H, *Naruse K, Kondo M, Takeda N, Iwata S, Hasegawa Y and Sokabe M

Am J Respir Cell Mol Biol 38 ( 4 ) 407 - 413 2008年4月

Cyclic strain induces mouse embryonic stem cell differentiation into vascular smooth muscle cells by activating PDGF receptor β 査読

Shimizu N, Yamamoto K, Obi S, Kumagaya S, Masumura T, Shimano Y, Naruse K, Yamashita JK, Igarashi T, Ando J

J Appl Physiol 104 ( 3 ) 766 - 772 2008年3月

Effects of tensile and compressive strains on response of a chondrocytic cell line embedded in type I collagen gel. 査読

Hirano Y, Ishiguro N, Sokabe M, Takigawa M and *Naruse K

J Biotechnol 133 ( 2 ) 245 - 252 2008年1月

Analysis of cyclic-stretching responses using cell-adhesion-patterned cells. 査読

Katanosaka Y, Bao JH,Komatsu T, Suemori T, Yamada A, Mohri S and *Naruse K

J Biotechnol 133 ( 1 ) 82 - 89 2008年1月

Impaired NO-mediated vasodilation with increased superoxide but robust EDHF function in right ventricular arterial microvessels of pulmonary hypertensive rats. 査読

Kajiya M, Hirota M, Inai Y, Kiyooka T, Morimoto T, Iwasaki T, Endo K, Mohri S, Shimizu J, Yada T, Ogasawara Y, *Naruse K, Ohe T and Kajiya F

Am J Physiol 292 ( 6 ) H2737 - H2744 2007年6月

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