Faculty Profiles - HATABU Toshimitsu

MISC - HATABU Toshimitsu

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P164 Study on vitamin A uptake of Plasmodium falciparum in vitro

MIZUNO Yasutaka, KAWAZU Shin-ichiro, HATABU Toshimitsu, KOMAKI Kanako, ISHIKAWA Hiroyuki, KANO Shigeyuki, OHTOMO Hiroshi

Med. Entomol. Zool. 52 ( 0 ) 118 - 118 2001

P166 Production of transgenic Plasmodium falciparum overexpressing an anti-oxidant enzyme, peroxiredoxin

KOMAKI Kanako, KAWAZU Shinichiro, HATABU Toshimitsu, ISHIKAWA Hiroyuki, KANO Shigeyuki

Med. Entomol. Zool. 52 ( 0 ) 119 - 119 2001

P94W4-4 Growth inhibition of Plasmodium falciparum by treatment with anti-recombinant Pf-enolase IgG

ISHIKAWA Hiroyuki, KAWAZU Shin ichiro, HATABU Toshimitsu, KOMAKI Kanako, IKENOUE Nozomu, SUZUKI Mamoru, KANO Shigeyuki

Med. Entomol. Zool. 52 ( 0 ) 101 - 101 2001

CD4(+) cells are indispensable for ulcer development in murine cutaneous leishmaniasis

M Terabe, T Kuramochi, M Ito, T Hatabu, C Sanjoba, KP Chang, T Onodera, Y Matsumoto

INFECTION AND IMMUNITY 68 ( 8 ) 4574 - 4577 2000.8

Language：English Publisher：AMER SOC MICROBIOLOGY

One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4(+), CD8(+), or B220(+) cell populations and the remaining cells were injected into Leishmania-infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4(+) cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4(+) cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.

DOI： 10.1128/IAI.68.8.4574-4577.2000

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Molecular cloning and characterization of a peroxiredoxin from the human malaria parasite Plasmodium falciparum

S Kawazu, N Tsuji, T Hatabu, S Kawai, Y Matsumoto, S Kano

MOLECULAR AND BIOCHEMICAL PARASITOLOGY 109 ( 2 ) 165 - 169 2000.7

Cultivation of Plasmodium falciparum Isolates Under the Anaeropack Gas Condition in a Portable Thermostatic Incubator.

MIZUNO YASUTAKA, HATABU TOSHIMITSU, KAWAZU SHIN-ICHIRO, MASUDA GOHTA, OHTOMO HIROSHI, SUZUKI MAMORU, KANO SHIGEYUKI

Tropical Medicine and Health 23: 393-399 ( 4 ) 383 - 385 2000

Cultivation of Plasmodium falciparum isolates under the AnaeroPack gas condition in a portable thermostatic incubator.

HATABU, TOSHIMITSU

Japanese Journal of Tropical Medicine and Hygine 23: 393-399 ( 4 ) 383 - 385 2000

改良型ポータブル恒温槽及び酸素吸収・炭酸ガス発生剤(アネロパックTM)を用いた熱帯熱マラリア原虫の培養

畑生俊光, 水野泰孝, 河津信一郎, 鈴木守, 狩野繁之

日本獣医学会学術集会講演要旨集 128回 161 - 161 1999.9

Leishmania amazonensis infection in nude mice

M Terabe, T Hatabu, H Takahashi, M Ito, T Onodera, Y Matsumoto

EXPERIMENTAL ANIMALS 48 ( 2 ) 119 - 123 1999.4

Language：English Publisher：INT PRESS EDITING CENTRE INC

Leishmania amazonensis is an intracellular protozoan parasite of macrophages. Cutaneous leishmaniasis in an immunocompetent host begins as papules or nodules followed by ulceration at the site of promastigote inoculation. In this study, the pathological changes of cutaneous leishmaniasis lesions in T cell deficient nude mice were examined. When infected with L. amazonensis promastigotes, nude mice developed non-ulcerative cutaneous nodules, By histological examination of cutaneous lesions, massive accumulation of vacuolated histiocytes containing amastigotes was observed in all the nude mice. Although infiltration of mononuclear and polymorphonuclear cells was seen in the lesions of immunocompetent mice, few such cells were observed in the lesions of nude mice. These results indicate the importance of T cells on the ulcer formation in cutaneous leishmaniasis.

DOI： 10.1538/expanim.48.119

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LEISHMANIASIS IN CENTRAL EURASIA

MATSUMOTO YOSHITSUGU, HATABU TOSHIMITSU, SANJOBA CHIZU, MATSUMOTO YASUNOBU, SASAKI TETSUHIKO, KAWAZU SHIN-ICHIRO, NAKAI YUTAKA, KATAKURA KEN, ITO MAMORU, NAGAKURA KOICHI, AIKAWA MASAMICHI

Japanese journal of tropical medicine and hygiene 27 ( 1 ) 59 - 62 1999.3

Non-ulcerative cutaneous lesion in immunodeficient mice with Leishmania amazonensis infection

Takashi Kuramochi, Toshimitsu Hatabu, Mamoru Ito, Yoshihito Ueyama, Shin-Ichiro Kawazu, Takashi Onodera, Yoshitsugu Matsumoto

Parasitology International 48 ( 1 ) 47 - 53 1999.3

Language：English

Cutaneous leishmaniasis begins as papules or nodules at the site of promastigote inoculation. The next key pathogenic event in this disease is the formation of an ulcer at this site. Leishmania infection in immunodeficient mice, however, showed non-ulcerative cutaneous lesions suggesting the involvement of the immune system in ulcer formation. Severe combined immunodeficient (SCID), recombination-activating gene 2 knockout (RAG-2(-/-)), and immunocompetent mice were inoculated subcutaneously with cultured L. amazonensis promastigotes. Macroscopic nodules appeared at the inoculation site within 2 weeks of infection in all the mice and gradually extended to the surrounding skin tissue. Although nodules of immunocompetent mice ulcerated within 6 weeks, immunodeficient mice did not form ulcers even after 25 weeks of inoculation. These results strongly suggest the importance of functional T and B cells in ulcer formation of cutaneous leishmaniasis and are consistent with clinical features of non-ulcerative cutaneous leishmaniasis in some AIDS patients. The present study also indicates that the L. amazonensis-infected immunodeficient mouse model might be suitable for studying the mechanisms of ulcer formation in cutaneous leishmaniasis.

DOI： 10.1016/S1383-5769(98)00040-3

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リーシュマニア原虫を用いた哺乳動物遺伝子発現系の検討

中村優子, 畑生俊光, 松本安喜, 河津信一郎, Chang K.-P, 佐伯圭一, 小野寺節, 松本芳嗣

日本獣医学会学術集会講演要旨集 127回 93 - 93 1999.3

Leishmania spp.とζ-crystallin/NADPH oxidoreductase homologue(P36)遺伝子の比較

河津信一郎, 片倉賢, 永倉貢一, 畑生俊光, 伊藤守, 中井裕, 相川正道, Chang K.-P, 神尾次彦, 狩野繁之, 松本芳嗣

日本獣医学会学術集会講演要旨集 127回 93 - 93 1999.3

Leishmania mini-exon genes for molecular epidemiology of leishmaniasis in China and Ecuador

Ken Katakura, Shin-Ichiro Kawazu, Chizu Sanjyoba, Toshimitsu Naya, Yoshitsugu Matsumoto, Mamoru Ito, Koichi Nagakura, Masamichi Aikawa, Yoshihisa Hashiguchi

Tokai Journal of Experimental and Clinical Medicine 23 ( 6 ) 393 - 399 1998.12

Language：English

The mini-exon gene is unique and is tandemly repeated in the Leishmania genome. The transcribed region is highly conserved, but the non-transcribed spacer region is distinct in length and in sequence among different Leishmania species. The usefulness of PCR amplification of the Leishmania mini-exon gene was examined for molecular epidemiology of visceral and cutaneous leishmaniasis. We previously described a PCR method for amplification of the mini-exon gene and obtained positive amplification in bone marrow aspirates of patients with visceral leishmaniasis in China. In this study, we have cloned and sequenced two PCR products from the patients. The sequences of two products revealed 100% identity and showed more similarity to the mini-exon gene of L. donovani Indian strain than those of L. donovani complex in Africa and South America. We also applied this PCR method to the diagnosis of cutaneous leishmaniasis. We obtained positive PCR amplification in skin biopsy materials taken from patients with cutaneous leishmaniasis in Ecuador. Since this PCR amplification is simple and requires only a pair of primers to detect all Leishmania species distributed in Ecuador, the method may be a useful tool for the detection of parasites, not only from patients, but also from sandflies and reservoir animals in this area of endemicity.

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Diagnosis of kala-azar by nested PCR based on amplification of the Leishmania mini-exon gene

K Katakura, S Kawazu, T Naya, K Nagakura, M Ito, M Aikawa, JQ Qu, LR Guan, XP Zuo, JJ Chai, KP Chang, Y Matsumoto

JOURNAL OF CLINICAL MICROBIOLOGY 36 ( 8 ) 2173 - 2177 1998.8

Language：English Publisher：AMER SOC MICROBIOLOGY

To diagnose visceral leishmaniasis (kala-azar), we have developed a nested PCR method based on amplification of the mini-exon gene, which is unique and tandomly repeated in the Leishmania genome. Nested PCR was sufficiently sensitive for the detection of DNA in an amount equivalent to a single Leishmania parasite or less. We examined the usefulness of this PCR method using bone marrow aspirates and buffy coat cells collected from kala-azar patients who had or had not received chemotherapy in northwest China. We obtained PCR positivity for all of the parasitologically positive bone marrow samples from the patients. Some ambiguities with the primary PCR results were eliminated by the subsequent nested PCR. The buffy coat samples from 7 of 12 patients with splenomegaly were positive by the nested PCR, although only 2 of them were positive for parasites by culture. However, buffy coat samples from nine children, whose splenomegaly has been reduced and clinically cured by antimony treatment, were all negative. Thus; this nested PCR method represents a new tool for the diagnosis of kala-azar,vith patient blood samples instead of bone marrow or spleen aspirates obtained by more invasive procedures.

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Membrane knobs of unfixed Babesia bovis-infected erythrocytes: New findings as revealed by atomic force microscopy and surface potential spectroscopy

Masamichi Aikawa, Shin-Ichiro Kawazu, Tujihiko Kamio, Yoshitsugu Matsumoto, Toshimitsu Naya, Motomi Torii, Yoshihiro Ito, Bernard Tandler, Yamaji Nakano, Tsutomu Takeuchi, Soshi Shiraishi, Kiyoshi Kanamura

Parasitology International 46 ( 4 ) 241 - 246 1997

Language：English Publisher：Elsevier Ireland Ltd

Atomic force microscopy (AFM) and surface potential microscopy (SPS) were used to examine the structure of the knobs of unfixed Babesia bovis-infected erythrocytes, AFM revealed each knob was found to consist of two subunits, components that have not been observed in chemically fixed knobs by transmission electron microscopy. In addition, SPS revealed the knob surface has a positive electrical charge and that the remainder of the erythrocyte surface has a negative charge. These factors might be central to the phenomenon of cytoadherence in cerebral babesiosis. © 1997 Elsevier Science Ireland Ltd.

DOI： 10.1016/S1383-5769(97)00031-7

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