Faculty Profiles - HATABU Toshimitsu

MISC - HATABU Toshimitsu

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子宮内膜における免疫系は暑熱ストレスにより変化する

酒井駿介, 畑生俊光, 山本ゆき, 木村康二

The Journal of Reproduction and Development 64 ( Suppl. ) j83 - j83 2018.9

Eimeria tenella 弱毒株の作出と比較ゲノム解析による弱毒化分子機構の解明

松林誠, 川原史也, 山岸潤也, 八田岳士, 畑生俊光, 山口浩貴, 寺本勲, 金子明, 磯部尚, 北潔, 辻尚利, 笹井和美

日本獣医学会学術集会講演要旨集 161回 316 - 316 2018.8

ニワトリコクシジウム症アイメリア・テネラ感染メカニズムの解明に向けて

畑生俊光

岡山実験動物研究会報 ( 34 ) 80 - 80 2018.4

Eimeria tenella弱毒株の作出および病理組織学的比較解析

山口浩貴, 松林誠, 川原史也, 八田岳士, 畑生俊光, 山岸潤也, 磯部尚, 寺本勲, 金子明, 北潔, 辻尚利, 笹井和美

日本獣医学会学術集会講演要旨集 160回 336 - 336 2017.8

南米型トリパノソーマ感染細胞におけるオートファゴソーム形成と関連タンパク質の解析

高橋千由紀, 畑生俊光, 嶋田淳子

The Kitakanto Medical Journal 63 ( 3 ) 327 - 327 2013.8

ネズミマラリア感染臓器におけるスカベンジャー受容体の発現解析

宮下大地, 畑生俊光, 嶋田淳子

The Kitakanto Medical Journal 63 ( 3 ) 327 - 327 2013.8

Plasmodium berghei感染マウス臓器におけるスカベンジャー受容体SRCLの発現解析

宮下大地, 畑生俊光, 嶋田淳子

関東甲信地区医学検査学会講演集 48回 145 - 145 2011.10

南米型トリパノソーマ感染細胞におけるオートファジーとアポトーシスとの関連性の解析

高橋千由紀, 畑生俊光, 嶋田淳子

関東甲信地区医学検査学会講演集 48回 144 - 144 2011.10

群馬県における肺吸虫の分布調査群馬県内利根川水系産サワガニにおける肺吸虫類メタセルカリア寄生状況について

富澤将太, 畑生俊光, 嶋田淳子

関東甲信地区医学検査学会講演集 48回 145 - 145 2011.10

Trypanosoma cruzi infection induces nitric oxide production and S-nitrosylation of cellular FLIP in host cell

Junko Nakajima-Shimada, Toshimitsu Hatabu

Medimond S.r.l 2011

菌類代謝産物の抗マラリア活性(1)

内山奈穂子, 畑生俊光, 嶋田淳子, 川原信夫, 板橋武史, 河合賢一

日本生薬学会年会講演要旨集 56回 208 - 208 2009.9

高校生における麻疹予防接種歴調査、抗体価測定と予防教育

豊島幸子, 畑生俊光, 嶋田淳子, 大平孝雄, 八木秀明

The Kitakanto Medical Journal 59 ( 3 ) 325 - 325 2009.8

Association of molecular markers in Plasmodium falciparum crt and mdr1 with in vitro chloroquine resistance: a Philippine study. Reviewed International journal

Toshimitsu Hatabu, Moritoshi Iwagami, Shin-ichiro Kawazu, Nao Taguchi, Aleyla D Escueta, Elena A Villacorte, Pilarita T Rivera, Shigeyuki Kano

Parasitology international 58 ( 2 ) 166 - 70 2009.6

Language：English Publisher：ELSEVIER IRELAND LTD

Specific mutations in the pfcrt and pfmdr1 genes have been reported to be associated with chloroquine-resistant falciparum malaria parasites worldwide. These genetic markers are considered to be useful tools for the elucidation of several aspects of the epidemiology of drug resistant malaria. In this study, Plasmodium falciparum isolates from three distinct areas of the Philippines were analyzed for drug-resistance-associated genetic mutations, and their association with the in vitro chloroquine (CQ) response. Two novel pfcrt 72-76 allelic types, CVMDT and SVMDT, were detected. The frequency of the pfcrt K76T mutation in the isolates that were successfully tested for in vitro CQ susceptibility was found to be 100% in Kalinga, 80% in Palawan, and 87% in Mindanao. The frequency of the pfmdr1 N86Y mutation was 39% in Kalinga, 35% in Palawan, and 93% in Mindanao isolates. No mutations were found at positions 1042 and 1246 of pfmdr1. However, there were no significant associations found between polymorphisms in these genes and in vitro CQ susceptibility. The results of this study indicate that mutations in pfcrt and pfmdr1 are not predictive of in vitro CQ resistance in Philippine isolates and may therefore not be suitable as molecular markers for surveillance.

DOI： 10.1016/j.parint.2009.01.010

Genetic diversity and population structure of Plasmodium falciparum in the Philippines. Reviewed International journal

Moritoshi Iwagami, Pilarita T Rivera, Elena A Villacorte, Aleyla D Escueta, Toshimitsu Hatabu, Shin-ichiro Kawazu, Toshiyuki Hayakawa, Kazuyuki Tanabe, Shigeyuki Kano

Malaria journal 8 96 - 96 2009.5

Language：English Publisher：BIOMED CENTRAL LTD

BACKGROUND: In the Philippines, malaria morbidity and mortality have decreased since the 1990s by effective malaria control. Several epidemiological surveys have been performed in the country, but the characteristics of the Plasmodium falciparum populations are not yet fully understood. In this study, the genetic structure of P. falciparum populations in the Philippines was examined. METHODS: Population genetic analyses based on polymorphisms of 10 microsatellite loci of the parasite were conducted on 92 isolates from three provinces (Kalinga, Palawan, and Davao del Norte) with different malaria endemicity. RESULTS: The levels of genetic diversity and the effective population sizes of P. falciparum in the Philippines were similar to those reported in the mainland of Southeast Asia or South America. In the low malaria transmission area (Kalinga), there was a low level of genetic diversity and a strong linkage disequilibrium (LD) when the single-clone haplotype (SCH) was used in the multilocus LD analysis, while in the high malaria transmission areas (Palawan and Davao del Norte), there was a high level of genetic diversity and a weak LD when SCH was used in the multilocus LD analysis. On the other hand, when the unique haplotypes were used in the multilocus LD analysis, no significant LD was observed in the Kalinga and the Palawan populations. The Kalinga and the Palawan populations were, therefore, estimated to have an epidemic population structure. The three populations were moderately differentiated from each other. CONCLUSION: In each area, the level of genetic diversity correlates with the local malaria endemicity. These findings confirm that population genetic analyses using microsatellite loci are a useful tool for evaluating malaria endemicity.

DOI： 10.1186/1475-2875-8-96

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フィリピンの熱帯熱マラリア原虫集団の遺伝的構造と流行度

石上盛敏, リベラピラリタ, ビラコルテエレーナ, エスクエタアレイラ, 畑生俊光, 河津信一郎, 早川敏之, 田邉和裄, 狩野繁之

Trop Med Health 125 2008.10

Phylogenetic relationship of Plasmodium falciparum populations in the Philippines

Moritoshi Iwagami, Toshimitsu Hatabu, Shin-ichiro Kawazu, Aleyla S. Escueta, Elena A. Villacorte, Pilarita Tongol-Rivera, Shigeyuki Kano

Southeast Asian Journal of Tropical Medicine and Public Health 39 ( 2 ) 200 - 204 2008.3

Language：English

Malaria is one of the major infectious diseases in the Philippines. It is being targeted for control through sustained early diagnosis, treatment and mosquito control. It is in this light that understanding the genetic background of the parasite population is important not only for basic biology of the organism but also for epidemiology and control of the disease. In the present study, molecular phylogenetic relationships of the 3 Plasmodium falciparum populations in the Philippines with the other populations in the world were inferred based on polymorphisms of 9 highly polymorphic microsatellite DNA loci in the parasite genome. A total of 92 P. falciparum isolates collected from 3 provinces (Kalinga, Palawan and Davao del Morte) in the Philippines, and 8 from other populations (3 African, 2 South American, 2 Papua New Guinean, and 1 Thai) that were previously reported, were used for the analysis. The phylogenetic tree showed that the 3 Philippine populations were genetically divergent from each other as compared to the other populations. The branching pattern of the tree suggests that the 3 Philippine populations were relatively close to the Thai population, rather than the Papua New Guinean populations, indicating that the ancestor of the 3 Philippines populations were introduced from Indochina peninsula, and not from countries located south of the Philippines such as Papua New Guinea or Indonesia.

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Phylogenetic relationship of Plasmodium falciparum populations in the Philippines International journal

Moritoshi Iwagami, Toshimitsu Hatabu, Shin-ichiro Kawazu, Aleyla S. Escueta, Elena A. Villacorte, Pilarita Tongol-Rivera, Shigeyuki Kano

Southeast Asian Journal of Tropical Medicine and Public Health 39 ( 2 ) 200 - 204 2008.3

Language：English

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節足動物媒介感染症の効果的な防除等の対策研究マラリア重症患者の管理および治療体制に関する研究

狩野繁之, 水野泰孝, 石上盛敏, 竹内勤, 奥浩之, 畑生俊光, 谷畑健生, LOOAREESUWAN Sornchai, KRUDSOOD Srivicha

節足動物媒介感染症の効果的な防除等の対策研究平成19年度総括・分担研究報告書 2008

熱帯熱マラリア原虫に対するアムホテリシンBの発育阻止機序の解析

畑生俊光, 畑生俊光, 川合覚, 山口武士, 山田圭一, 奥浩之, 片貝良一, 嶋田淳子, 狩野繁之

日本寄生虫学会大会プログラム・抄録集 76th 2007

Plasmodium falciparum: the fungal metabolite gliotoxin inhibits proteasome proteolytic activity and exerts a plasmodicidal effect on P. falciparum. Reviewed International journal

Toshimitsu Hatabu, Masaki Hagiwara, Nao Taguchi, Masayuki Kiyozawa, Mamoru Suzuki, Shigeyuki Kano, Kumiko Sato

Experimental parasitology 112 ( 3 ) 179 - 83 2006.3

Language：English Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE

The in vitro antimalarial activity of the fungal metabolite gliotoxin (GTX) was evaluated, and its mechanism of action was studied. GTX showed plasmodicidal activity against both Plasmodium falciparum chloroquine-resistant strain K-1 and chloroquine-susceptible strain FCR-3. GTX cytotoxicity was significantly lower against a normal liver cell line (Chang Liver cells). The intracellular reduced glutathione level of parasitized and of normal red blood cells was not affected by GTX treatment. However, GTX decreased the chymotrypsin-like activity of parasite proteasomes in a time-dependent manner. The results of this study indicate that GTX possesses plasmodicidal activity and that this effect is due to inhibition of parasite proteasome activity, suggesting that GTX may constitute a useful antimalarial therapy.

DOI： 10.1016/j.exppara.2005.11.012

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