Faculty Profiles - HATABU Toshimitsu

MISC - HATABU Toshimitsu

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PCR-amplification, sequencing, and comparison of the var/PfEMP-1 gene from the blood of patients with falciparum malaria in the Philippines.

HATABU, TOSHIMITSU

Southeast Asian Journal of Tropical Medicine and Public Health 33 suppl(3):8-13 2003

群馬大学公開講座実施報告。科学する心を育てようーミクロの世界を覗いてみようー

福田利夫, 佐藤久美子, 畑生俊光, 小河原はつ江, 佐竹史明, 吉田朋美

群馬保健学紀要 24 117 - 122 2003

薬剤耐性マラリアの分布に関する調査研究

畑生俊光

北関東医学会誌 53巻3号 ( 3 ) 327 - 328 2003

亜セレン酸ナトリウムが及ぼすマラリア原虫培養系への影響

田口直, 畑生俊光, 澁澤美奈, 石原亜矢, 佐藤久美子

関東甲信地区医学検査学会講演集 39回 121 - 121 2002.11

Severe Falciparum Malaria with Prolonged Hemolytic Anemia after Successful Treatment with Intravenous Artesunate

ITODA Ichiro, YASUNAMI Takeshi, KIKUCHI Ken, YAMAURA Hisashi, TOTSUKA Kyoichi, YOSHINAGA Kentaro, TERAMURA Masanao, MIZOGUCHI Hideaki, HATABU Toshimitsu, KANO Shigeyuki

76 ( 8 ) 600 - 603 2002.8

[Severe falciparum malaria with prolonged hemolytic anemia after successful treatment with intravenous artesunate]. Reviewed

Ichiro Itoda, Takeshi Yasunami, Ken Kikuchi, Hisashi Yamaura, Kyoichi Totsuka, Kentaro Yoshinaga, Masanao Teramura, Hideaki Mizoguchi, Toshimitsu Hatabu, Shigeyuki Kano

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases 76 ( 8 ) 600 - 3 2002.8

Language：Japanese

We report a 68-year-old woman with severe falciparum malaria contracted in Tanzania. She presented high parasitemia and was treated successfully with intravenous artesunate, a qinghaosu derivative, and aggressive supportive therapy. She developed hemolytic anemia and jaundice on day 11 and blood transfusion was required. This case illustrates that intravenous artesunate has excellent antimalarial activity with rapid efficacy and that no severe adverse effect but conventional aggressive supportive therapy is still important in the treatment of severe falciparum malaria.

DOI： 10.11150/kansenshogakuzasshi1970.76.600

ネズミマラリア原虫(Plasmodium yoelii)ペルオキシレドキシンの媒介蚊体内発育ステージにおける発現

河津信一郎, 野崎智義, 坪井敬文, 中野陽子, 畑生俊光, 鳥居本美, 狩野繁之

日本獣医学会学術集会講演要旨集 133rd 72 - 72 2002.3

ネズミマラリア原虫Plasmodium berghei強毒株(NK65),弱毒株(XAT)間におけるプロテオーム解析の試み

石川浩之, 河津信一郎, 畑生俊光, 駒木加奈子, 中野陽子, 鈴木守, 狩野繁之

日本寄生虫学会大会プログラム・抄録集 71st 114 2002.3

フィリピン・ミンダナオ島における熱帯熱マラリア原虫クロロキン感受性の検討

畑生俊光, 河津信一郎, 鈴木淳, VALENZUELA R F, VILLACORTE E A, RIVERA P T, 狩野繁之

日本寄生虫学会大会プログラム・抄録集 71st 118 2002.3

The expression system of biologically active canine interleukin-8 in Leishmania promastigotes

T Hatabu, Y Matsumoto, S Kawazu, Y Nakamura, T Kamio, HG Lu, KP Chang, Y Hashiguchi, S Kano, T Onodera, Y Matsumoto

PARASITOLOGY INTERNATIONAL 51 ( 1 ) 63 - 71 2002.3

Language：English Publisher：ELSEVIER IRELAND LTD

It has been reported that Leishmania promastigotes have ability to express foreign genes on drug selectable plasmids. To investigate further abilities of the recently described expression vector, P6.5, in the transfection of Leishmania organisms (Chen D-Q, Kolli BK, Yadava N et al. Episomal expression of specific sense and antisense mRNAs in Leishmania amazonensis: modulation of gp63 levels in promastigotes and their infection of macrophages in vitro. Infect Immun 2000;68:80-86). the constructed expression vector, which contains canine interleukin-8 (cIL-8) coding cDNA, was introduced by electroporation to promastigotes of four species of the genus Leishmania: Leishmania amazonensis, L. equatorensis, L. donovani and L. infantum. Extrachromosomal DNAs and total RNAs from the transfected promastigotes were subjected to polymerase chain reaction (PCR) and reverse transcriptase-PCR, respectively, using cIL-8 gene specific primers, and a predicted product of 330 bp was detected. Western blot analysis using a mouse monoclonal antibody raised against cIL-8 demonstrated the successful expression of cIL-8 in the transfectants and culture supernatants. Culture supernatants of the transfected L. amazonensis and L. equatorensis promastigotes showed a high chemotactic activity to both dog and mouse polymorphonuclear leukocytes. These results indicate that Leishmania promastigotes transfected with the expression vector P6.5 containing cIL-8 cDNA are capable of producing biologically active cIL-8. The Leishmania expression system using the P6.5 vector might be a useful alternative for the production of biologically active recombinant cytokines. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

DOI： 10.1016/S1383-5769(01)00107-6

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The expression system of biologically active canine interleukin-8 in Leishmania promastigotes. Reviewed International journal

Toshimitsu Hatabu, Yasunobu Matsumoto, Shin-ichiro Kawazu, Yuko Nakamura, Tsugihiko Kamio, Hong Gang Lu, Kwang-Poo Chang, Yoshihisa Hashiguchi, Shigeyuki Kano, Takashi Onodera, Yoshitsugu Matsumoto

Parasitology international 51 ( 1 ) 63 - 71 2002.3

Language：English Publisher：ELSEVIER IRELAND LTD

It has been reported that Leishmania promastigotes have ability to express foreign genes on drug selectable plasmids. To investigate further abilities of the recently described expression vector, P6.5, in the transfection of Leishmania organisms (Chen D-Q, Kolli BK, Yadava N et al. Episomal expression of specific sense and antisense mRNAs in Leishmania amazonensis: modulation of gp63 levels in promastigotes and their infection of macrophages in vitro. Infect Immun 2000;68:80--86), the constructed expression vector, which contains canine interleukin-8 (cIL-8) coding cDNA, was introduced by electroporation to promastigotes of four species of the genus Leishmania: Leishmania amazonensis, L. equatorensis, L. donovani and L. infantum. Extrachromosomal DNAs and total RNAs from the transfected promastigotes were subjected to polymerase chain reaction (PCR) and reverse transcriptase-PCR, respectively, using cIL-8 gene specific primers, and a predicted product of 330 bp was detected. Western blot analysis using a mouse monoclonal antibody raised against cIL-8 demonstrated the successful expression of cIL-8 in the transfectants and culture supernatants. Culture supernatants of the transfected L. amazonensis and L. equatorensis promastigotes showed a high chemotactic activity to both dog and mouse polymorphonuclear leukocytes. These results indicate that Leishmania promastigotes transfected with the expression vector P6.5 containing cIL-8 cDNA are capable of producing biologically active cIL-8. The Leishmania expression system using the P6.5 vector might be a useful alternative for the production of biologically active recombinant cytokines.

DOI： 10.1016/S1383-5769(01)00107-6

Web of Science

PubMed

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PCR-amplification, sequencing, and comparison of the var/PfEMP-1 gene from the blood of patients with falciparum malaria in the Philippines. Reviewed International journal

Nozomu Ikenoue, Shin-ichiro Kawazu, Toshimitsu Hatabu, Elena A Villacorte, Pilarita T Rivera, Shigeyuki Kano

The Southeast Asian journal of tropical medicine and public health 33 Suppl 3 8 - 13 2002

Language：English

The adhesion of Plasmodium falciparum-infected erythrocytes to vascular endothelium and to uninfected red blood cells (RBCs) plays a key role in the pathology of severe malaria. Adhesion is known to be mediated in part by the antigenically-variant erythrocyte membrane protein-1 (PfEMP-1), which is encoded by the var-gene family of P. falciparum. It has recently been reported that in vitro a single parasite simultaneously transcribes multiple var-genes but that, through a developmentally regulated process, the parasite selects only one PfEMP-1 that will to reach the surface of the host RBC. Were this to be true in vivo, one would expect a correlation between the type of var/PfEMP-1 that is expressed on the parasite-infected RBC and the severity of clinical disease. In order to test this assumption, we determined the sequence of the var-gene that was expressed by the parasites in patients' blood samples. Seven blood samples were collected from patients with or without severe clinical symptoms (cerebral malaria): two samples were from patients diagnosed as having imported falciparum malaria at the International Medical Center of Japan (IMCJ); the five others were from patients of the Davao Regional Hospital in Davao, the Philippines. The parasites (ring stage) in the blood samples were cultured for 24 hours; the matured trophozoites, in which the var-gene selection had taken place, served as material for mRNA isolation. The cDNA corresponding to the Duffy-binding-like (DBL)-1 domain of the var-gene was amplified by RT-PCR, using a region-specific primer set. The amplified cDNAs were cloned into the plasmid vector; the resultant clones (32) were sequenced on both strands. The results indicated that there was considerable diversity in the sequence of the DBL-1 domain among the clones, even among those from a single patient. In conclusion, it was difficult to demonstrate the correlation between the type of var-gene transcripts found in the RBCs of malaria patients and the severity of their symptoms.

PubMed

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フィリピン・ミンダナオ島における熱帯熱マラリア原虫の薬剤感受性酸素吸収・炭酸ガス発生剤(アネロパック)を用いた薬剤感受性試験

畑生俊光, 鈴木淳, VALENZUELA R F, VILLACORTE E A, RIVERA R T, 河津信一郎, 狩野繁之

日本熱帯医学会雑誌 29 ( 増刊 ) 160 - 160 2001.8

Molecular characterization of a 2-Cys peroxiredoxin from the human malaria parasite Plasmodium falciparum

S Kawazu, K Komaki, N Tsuji, S Kawai, N Ikenoue, T Hatabu, H Ishikawa, Y Matsumoto, K Himeno, S Kano

MOLECULAR AND BIOCHEMICAL PARASITOLOGY 116 ( 1 ) 73 - 79 2001.8

Language：English Publisher：ELSEVIER SCIENCE BV

We have identified the 2-Cys peroxiredoxin (PfPrx-1) from the human malaria parasite Plasmodium falciparum. The PfPrx-1 showed the highest identity at amino acid level to the type Il Prx among the currently known six subfamilies of mammalian Prx. The sequence identity between the PfPrx-1 and the previously reported I-Cys Prx. of P. falciparum (PfPrx-2), which corresponded to mammalian type VI Prx, was 25%. This suggests that the parasite possesses two Prx subfamilies. The PfPrx-1 showed significant sequence similarities with those of 2-Cys peroxiredoxins of plants in the BLASTX search. This may reflect the consequences of a genetic transfer from an algal endosymbiont to the parasite nucleus during evolution. The recombinant PfPrx-1 protein (rPfPrx-1) was expressed as a histidine fusion protein in Escherichia coli and purified with Ni chromatography. The rPfPrx-1 existed as dimers under non-reducing conditions and dissociated into monomers in the presence of dithiothreitol. The PfPrx-1 protein also exists as a dimer in the parasites themselves. The reduction of the oxidized enzyme by the donation of electrons from E. coli thioredoxin (Trx)/Trx reductase system was demonstrated in its reaction with H2O2, using the rPfPrx-1 protein. These results suggested that the PfPrx-1 can act as a terminal peroxidase of the parasite Trx system. An elevated expression of the PfPrx-1 protein seen in the trophozoite, the stage with active metabolism, suggests an association of the parasite Trx. system with its intracellular redox control. (C) 2001 Elsevier Science B.V. All rights reserved.

DOI： 10.1016/S0166-6851(01)00308-5

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熱帯熱マラリア原虫(Plasmodium falciparum)2‐Cys型ペルオキシレドキシンの性状解析

河津信一郎, 駒木加奈子, 辻尚利, 川合覚, 池ノ上望, 石川浩之, 畑生俊光, 松本芳嗣, 狩野繁之

日本獣医学会学術集会講演要旨集 131st 65 - 65 2001.3

P181 Immunohistochemical analysis of liver and spleen in beige mutant mice infected with P. berghei XAT

HATABU Toshimitsu, ISHIKAWA Hiroyuki, KOMAKI Kanako, MIZUNO Yasutaka, FUKUDA Toshio, SUZUKI Mamoru, KAWAZU Shinichiro, KANO Shigeyuki

Med. Entomol. Zool. 52 ( 0 ) 123 - 123 2001

A case of falciparum malaria successfully treated with intravenus artesunete.

HATABU, TOSHIMITSU

感染症学雑誌 75(9): 822-5 2001

A case of falciparum malaria successfully treated with intravenus artesunete.

HATABU, TOSHIMITSU

感染症学雑誌 75(9): 822-5 2001

フィリピンミンダナオ島のマラリア流行調査ー携帯型連続培養システムを用いたマラリア原虫薬剤感受性試験ー

畑生俊光

熱帯第34巻187-196 2001

P165 Molecular cloning of 2-Cys peroxiredoxin from the human malaria parasite Plasmodium falciparum

KAWAZU Shinichiro, KOMAKI Kanako, TSUJI Naotoshi, KAWAI Satoru, ISHIKAWA Hiroyuki, HATABU Toshimitsu, MATSUMOTO Yoshitsugu, KANO Shigeyuki

Med. Entomol. Zool. 52 ( 0 ) 119 - 119 2001

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