論文 - 柳井　広之

Regulation of Interleukin-2 Receptor γ Chain mRNA Expression in Human Monocytic Cell Line THP-I 査読

Hiroyuki Yanai, Tadashi Yoshino, Kiyoshi Takahashi, Yoshifumi Ninomiya, Tadaatsu Akagi

Acta Medica Okayama   50 ( 3 )   145 - 150   1996年6月

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

The interleukin-2 receptor (IL-2R) γ chain (γc chain) is shared by IL-4R, IL-7R, IL-9R, and IL-15R and plays an important role in regulation of the immune system. However, its regulation in monocytic cell lines has not been well clarified. We examined the expression and regulation of the IL-2Rα, IL-2Rβ, γc chain, IL-4R and IL-7R mRNA in a human monoblastic leukemia cell line, THP-1. Unstimulated THP-1 cells constitutively expressed a low level of γc chain and IL-4R mRNA, Phorbol myristate acetate (PMA) induced macrophage-like differentiation and up-regulated the γc chain mRNA expression in THP-1 cells. This effect of PMA was suppressed by the protein kinase inhibitors H-7 and staurosporine. PMA did not affect the expression of the other IL-R mRNAs examined. 1 α, 25(OH)2D3 and interferon-γ also induced differentiation of THP-1 cells, but these reagents did not affect the expression of the IL-R mRNAs in THP-1 cells. These findings suggest that the expression of the γc chain mRNA is regulated by the PMA-dependent pathway and is not associated with that of the other IL-R mRNAs.

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Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44 査読

Nobuhiro Kawasaki, Yoshinobu Matsuo, Tadashi Yoshino, Hiroyuki Yanai, Takashi Oka, Norihiro Teramoto, Cao Liu, Eisaku Kondo, Jun Minowada, Tadaatsu Akagi

Japanese Journal of Cancer Research   87 ( 10 )   1070 - 1077   1996年

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japanese Cancer Association  

To investigate whether the lymphocyte homing receptors, adhesion molecules regulating normal lymphocyte traffic, influence the dissemination of lymphoma cells, 24 lymphoma/leukemia cell lines were inoculated into SCID mice subcutaneously, and the correlation between the expression of the adhesion molecules and the metastatic potential of the cell lines was examined. Among the six adhesion molecules examined (LFA-1, ICAM-1, CLA, VLA-4, L-selectin and CD44), L-selectin increased the incidence of lymph node metastasis, and CD44 expression was related to both lymph node and organ (hematogenous) metastasis. A monoclonal antibody to the standard form of CD44 (CD44s), Hermes-3, inhibited the local growth and remote metastasis of CD44+ cell lines. Thus, it is concluded that at least CD44s expression is important in both lymphatic and hematogenous metastasis.

DOI： 10.1111/j.1349-7006.1996.tb03112.x

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Binding of human leukocytes to fibronectin is augmented by an anti-CD44 mAb (TL-1) and blocked by another anti-CD44 mAb (Hermes-3) but not by anti-VLA-4/VLA-5 mAbs 査読

Liu Cao, Tadashi Yoshino, Nobuhiro Kawasaki, Hiroyuki Yanai, Kunimitsu Kawahara, Eisaku Kondo, Kunihiro Omonishi, Kiyoshi Takahashi, Tadaatsu Akagi

Immunobiology   196 ( 5 )   504 - 512   1996年

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier GmbH  

Fibronectin (FN) forms meshworks in extracellular spaces, and it plays an important role in cellular trafficking. Lymphoid cells are activated by binding to FN of the VLA-4 and VLA-5 receptors. CD44 also acts as a receptor of FN, but the mechanism and physiologic regulation of their binding are poorly understood. We have developed an anti-CD44 monoclonal antibody (mAb) (TL-1) in which lymphoid cells are activated and form homotypic cell aggregation. In this study, we found that the adhesion of GEM, HSB2, and LAD lymphoid cells to FN was augmented by TL-1 treatment and was apparently blocked by another anti-CD44 mAb (Hermes-3), but TL-1 Fab' fragments treatment did not induce FN-binding. A similar phenomenon is reported in the binding of the CD44 molecule to HA. This augmentation was not inhibited by the CS1 and RGD peptides of FN or by anti-VLA-4 and -VLA-5 mAbs
it was energy-dependent and associated with cytoplasmic actin filaments. Tl-1 treatment did not alter the cell surface expression of CD44 molecules. These findings above suggested that activated and/or altered cell surface distribution of CD44 molecules via a conformational change augmented the avidity of its binding to FN, which may be similar to lymphocyte-hyaluronate and lymphocyte-endothelial cell binding. As the Hermes-3 binding site is also involved in the interaction between lymphocytes and endothelial cells, activation of lymphocytes via CD44 molecules may facilitate the binding of lymphocytes to endothelial cells, extravasation, and migration to inflammatory sites rich in FN.

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Thrombotic Thrombocytopenic Purpura and Myoglobinuric Acute Renal Failure Following Radiation Therapy in a Patient with Polymyositis and Cervical Cancer 査読

Hirofumi Makino, Yoshio Nagake, Kazuhiko Moriwaki, Shuzo Hirakawa, Zensuke Ota, Takaaki Katayama, Hiroyuki Yanai, Kiyoshi Takahashi, Tadaatsu Akagi

Internal Medicine   34 ( 1 )   24 - 27   1995年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

73-year-old woman was admitted to receive radiation treatment for uterine cervical cancer, however a complex series of events ensued, leading to death. She developed an acute exacerbation of polymyositis complicated by thrombotic thrombocytopenic purpura, rhabdomyolysis and acute renal failure. Radiation therapy may have produced an immune disturbance leading to the acute exacerbation of polymyositis. Auto-immune-mediated endothelial damage might have triggered a series of events leading to thrombotic thrombocytopenic purpura. Rhabdomyolysis seemed to be the main cause of acute renal failure. (Internal Medicine 34: 24-27, 1995). © 1995, The Japanese Society of Internal Medicine. All rights reserved.

DOI： 10.2169/internalmedicine.34.24

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膀胱のcarcinosarcomaの一例

柳井広之, 岡本 司, 松木 暁

岡山外科病理雑誌   32   72 - 73   1995年

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担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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Partial Purification and Chraracterization of Dendritic Cell Differentiation Factor

Miyatani Katsuya, Takahashi Kiyoshi, Yanai Hiroyuki, Yoshino Tadashi, Akagi Tadaatsu

Acta Medica Okayama   48 ( 2 )   67 - 72   1994年4月

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記述言語：英語   出版者・発行元：Okayama University Medical School  

<p>Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic leukemia cells and we referred to this factor as dendritic cell differentiation factor (DCDF). In this study, we attempted to purify and characterize DCDF and investigated its biological effect on normal human monocytes. Gel filtration chromatography indicated that the molecular weight of DCDF is approximately 30-35 kDa. Chromatofocusing indicated that the isoelectric point of DCDF is approximately 5.0. DCDF, partially purified by subsequent gel filtration, chromatofocusing, and hydrophobic chromatography, significantly enhanced the HLA-DR expression of normal human monocytes and a human monocytic leukemia cell line, THP-1. This biological activity was not neutralized by any known antibodies to human cytokines. DCDF significantly amplified the T-cell stimulatory activity of monocytes in the allogeneic mixed leukocyte reaction (MLR). Moreover, DCDF significantly enhanced IL-1 beta and IL-6 production by monocytes in a dose-dependent manner. These results suggest that DCDF is a novel human cytokine which stimulates the accessory cell function of monocytes.</p>

DOI： 10.18926/AMO/31108

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卵巣の硬化性間質性腫瘍の1例

柳井広之, 岡本 司, 平林光司, 早瀬良二, 赤木忠厚

岡山外科病理雑誌   31   33 - 36   1994年

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担当区分：筆頭著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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Induction of interdigitating reticulum cell-like differentiation in human monocytic leukemia cells by conditioned medium from IL-2-stimulated helper T-cells 査読

Kiyoshi Takahashi, Katsuya Miyatani, Hiroyuki Yanai, Ho Jong Jeon, Kotaro Fujiwara, Tadashi Yoshino, Kazuhiko Hayashi, Tadaatsu Akagi, Ken Tsutsui, Koichi Mizobuchi

Virchows Archiv B Cell Pathology Including Molecular Pathology   62 ( 1 )   105 - 113   1992年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer-Verlag  

Monocytic leukemia (MoL) cells were obtained from the peripheral blood of a patient in whom the leukemic cells infiltrating various lymphoreticular organs exhibited features intermediate between interdigitating reticulum cells (IDC) and ordinary phagocytic macrophages, whereas the leukemic cells in the peripheral blood were essentially monocytic and lacked such features. Peripheral blood CD4+ T-cells were established as an interleukin-2-dependent T-cell line. When the MoL cells were exposed for a few days to conditioned medium from the T-cell line, they extended several dendritic cytoplasmic projections and became intensely positive for HLA-DR antigen, cytoplasmic S-100β protein, and CD1 antigen. Functionally, the conditioned medium significantly down-regulated Fc-mediated and Fc-independent phagocytic activities, and the levels of lysosomal enzymes such as lysozyme and nonspecific esterase in the MoL cells. Moreover, the conditioned medium significantly up-regulated the accessory cell function of the MoL cells as measured by the primary allogenic mixed leukocyte reaction (MLR). Furthermore, the conditioned medium significantly down-regulated the expression of CD14 antigen. Biochemical analysis indicated that the factor responsible for these changes is a protein which is distinct from known human cytokines and whose molecular weight is approximately 31 kDa. These findings suggest that IDC are closely related the monocytic lineage and that helper T-cells play an important role in constructing the microenvironment of T-lymphoid tissues which is necessary for the differentiation and maturation of IDC. © 1992 Springer-Verlag.

DOI： 10.1007/BF02899671

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