研究者詳細 - 松本　卓也

MISC - 松本　卓也

分割表示 98 件中 81 - 98 件目／全件表示 >>

Hydroxyapatite particles as a controlled release carrier of protein

T Matsumoto, M Okazaki, M Inoue, S Yamaguchi, T Kusunose, T Toyonaga, Y Hamada, J Takahashi

BIOMATERIALS 25 ( 17 ) 3807 - 3812 2004年8月

記述言語：英語出版者・発行元：ELSEVIER SCI LTD

This study examines the possibility of using hydroxyapatite (HAp) particles as a controlled release carrier of protein. In order to achieve effective protein release from HAp particles, it is necessary to regulate the conjugated amount of protein on HAp and the resorption of HAp. HAp particles were synthesized at different temperatures (40 degreesC, 60 degreesC, 80 degreesC) in wet condition and the physicochemical properties of synthesized HAp particles were examined. HAp particles synthesized at low temperatures showed low crystallinity, high solubility and large specific surface area. The useful growth factors for bone regeneration, such as BMP, bFGF and TGF-beta, are basic proteins, so cytochrome c (pI = 10.2) was used as a model protein and the adsorptive property of protein on HAp particles was investigated. The protein adsorption on HAp particles changed depending on its specific surface area and the chart of protein adsorption on HAp particles showed a typical Langmuir curve. These findings suggest that the adsorbed amount of protein on HAp particles could be regulated by HAp synthesizing temperature and the concentrations of protein solution. The release kinetics of protein from the HAp particles that adsorbed the protein (HAp-pro) was also evaluated in different pH solutions (pH 4.0 and 7.0). The released protein gradually increased time dependently when HAp-pro were immersed in pH 4.0 solution, but the released protein was significantly smaller when HAp-pro were immersed in pH 7.0 solution. Moreover, the release rate of protein from HAp-pro differed in each HAp that was synthesized at different temperatures, suggesting that the release of protein from HAp-pro depended on HAp resorption. These results suggest that HAp particles synthesized at different temperature are useful as a controlled release carrier of protein. (C) 2003 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biomaterials.2003.10.081

SVVYGLR ペプチドにおける血管新生作用

濱田吉之輔, 松浦成昭, 軒原清史, 松本卓也, 藤谷渉, 高橋純造

歯科材料・器械 22 ( 5 ) 339 - 339 2003年8月

In vitro mineralization on alginate hydrogel membrane.

T. Toyonaga, T. Matsumoto, M. Inoue, T. Kusunose, S. Yamada, T. Nakamura, J. Takahashi

JOURNAL OF DENTAL RESEARCH 82 B215 - B215 2003年6月

Biodegradation of carbonate apatite/collagen composite membrane and its controlled release of carbonate apatite

T Matsumoto, M Okazaki, M Inoue, S Ode, C Chang-Chien, H Nakao, Y Hamada, J Takahashi

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH 60 ( 4 ) 651 - 656 2002年6月

記述言語：英語出版者・発行元：JOHN WILEY & SONS INC

The aim of this study was to investigate the biodegradation of carbonate apatite (CO(3)Ap)/collagen composite membrane as a new guided tissue regeneration membrane in vivo and to estimate its controlled release of CO(3)Ap in vitro. To control the biodegradation of the guided tissue regeneration membrane and to promote hard tissue regeneration in the periodontal region, we added CO(3)Ap into the collagen membrane. To investigate the biodegradation of CO(3)Ap/collagen composite membranes, the prepared membranes (CO(3)Ap:0, 10 wt % ) were cut into 5 x 5 x 0.1 mm and subcutaneously implanted into the backside of male rats under general anesthesia. The explanted membranes were investigated histologically. To estimate their controlled release of CO(3)Ap in vitro, the membranes (CO(3)Ap 0-10 wt %, 5 x 5 x 0.1 mm) were immersed into collagenase solution and compulsorily dissolved for 48 h. Histological results suggested that the membrane had a good biocompatibility and the biodegradable period was shortened with the presence of CO(3)Ap. In the solubility experiments of the membrane, eluted Ca concentrations gradually increased with total dependence on the dissolution of the collagen membrane, Our study demonstrated that the biodegradation time can be controlled by CO(3)Ap contents in the membrane and CO(3)Ap could be released from the membrane with the biodegradation period, (C) 2002 Wiley Periodicals, Inc.

DOI： 10.1002/jbm.10133

Web of Science

PubMed

researchmap

Crystallinity and solubility characteristics of hydroxyapatite adsorbed amino acid

T Matsumoto, M Okazaki, M Inoue, Y Hamada, M Taira, J Takahashi

BIOMATERIALS 23 ( 10 ) 2241 - 2247 2002年5月

記述言語：英語出版者・発行元：ELSEVIER SCI LTD

Hydroxyapatite (HAp) was synthesized in the presence of a variety of amino acids in order to investigate the effect of amino acid on the crystallinity and the solubility characteristics of HAp in the HAp-synthesizing condition. In the results of X-ray diffraction analysis. HAp synthesized in the presence of glycine (HAp-Gly). serine (HAp-Ser). aspartic acid (HAp-Asp) and glutamic acid (HAp-Glu) showed poor crystallinity compared with HAp synthesized in the absence of amino acid (HAp-con). The results of Fourier transform infrared spectroscope suggested the adsorption of these amino acids on HAp. Scanning electron microphotographs showed that the size and morphology of HAp adsorbed amino acids changed significantly. Furthermore, the solubility of these HAps increased significantly compared to HAp-con, each differing in four amino acids, However. other amino acids did not affect the crystallinity and morphology of HAp and had no significant change in their solubility. Collectively. this study suggests that the crystallinity and the solubility of synthesized HAp are different owing to the, variation of amino acids in the HAp synthesizing condition. It is expected that digestion-regulated HAp materials could be synthesized by using amino acid in their synthesizing condition. (C), 2002 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S0142-9612(01)00358-1

Low crystalline hydroxyapatite as the controlled release carrier of basic protein

T Matsumoto, M Okazaki, M Inoue, S Yamaguchi, T Kusunose, Y Hamada, J Takahashi

JOURNAL OF DENTAL RESEARCH 81 A191 - A191 2002年3月

骨再生用多孔質乳酸-ε-カプロラクトン共重合体の諸性質(その3)発泡体の分解性に及ぼす共重合比の影響

中尾浩之, 松本卓也, 高橋純造, 玄丞恷, 堤定美

歯科材料・器械 20 93 - 93 2001年9月

異なる結晶性を有するハイドロキシアパタイトへの塩基性タンパク質の固定

松本卓也, 岡崎正之, 中尾浩之, 濱田吉之輔, 高橋純造

歯科材料・器械 20 120 - 120 2001年9月

多孔質乳酸-ε-カプロラクトン共重合体の諸性質 : (その2)ポアーサイズ制御と分解性について

中尾浩之, 松本卓也, 高橋純造, 玄丞恷, 堤定美

歯科材料・器械 20 112 - 112 2001年3月

アパタイト含有コラーゲン膜の物理化学的特性

松本卓也, 岡崎正之, 張簡敬哲, 大出俊平, 平雅之, 高橋純造

歯科材料・器械 19 135 - 135 2000年9月

ゾルーゲル法応用による生分解性シリカーリン酸-カルシア系ガラスセラミックスの溶出性, 細胞親和性と生体内反応に関する研究

平雅之, 松本卓也, 北原一慶, 高橋純造, 瀧智弘, 岡崎正之, 荒木吉馬

歯科材料・器械 19 138 - 138 2000年9月

I型コラーゲンゲルを用いた石灰化実験

松本卓也, 岡崎正之, 高橋純造

歯科材料・器械 19 ( 35 ) 52 - 52 2000年4月

生体組織工学に関する研究 (第2報) ラット骨髄由来細胞の急速増殖と人工足場材料内での3次元培養・骨系分化の試み

平雅之, 中尾浩之, 松本卓也, 北原一慶, 高橋純造, 瀧智弘, 岡崎正之, 玄丞烋, 堤定美

歯科材料・器械 19 ( 35 ) 99 - 99 2000年4月

アパタイト生成におけるアミノ酸の影響

松本卓也, 岡崎正之, 平雅之, 高橋純造

歯科材料・器械 18 ( 33 ) 85 - 85 1999年3月

カスケード供給方式による積層型フッ素化アパタイトの合成

岡崎正之, 松本卓也, 平雅之, 高橋純造

歯科材料・器械 17 ( 32 ) 34 - 34 1998年9月

糖質存在下におけるアパタイト結晶の合成

松本卓也, 岡崎正之, 平雅之, 高橋純造

歯科材料・器械 17 ( 31 ) 100 - 100 1998年3月

生体材料としての結晶内に傾斜性を有する炭酸アパタイトの合成

岡崎正之, 松本卓也, 平雅之, 高橋純造

歯科材料・器械 17 ( 31 ) 102 - 102 1998年3月

生体組織工学に関する研究 (第1報) SDラットからの骨芽細胞の誘導培養とキャラクタリゼーション

平雅之, 中尾浩之, 松本卓也, 高橋純造, 瀧智弘, 岡崎正之

歯科材料・器械 18 ( 34 ) 243 - 243 1990年9月

　前のページ - 次のページ　

<松本 卓也>

MISC - 松本 卓也

<松本　卓也>

MISC - 松本　卓也