研究者詳細 - 松本　卓也

MISC - 松本　卓也

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Biomimetic fabrication of fibrin/apatite composite material

Rui Yoh, Takuya Matsumoto, Jun-ichi Sasaki, Taiji Sohmura

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 87A ( 1 ) 222 - 228 2008年10月

記述言語：英語出版者・発行元：WILEY-LISS

Bidirectional diffusion of the Ca and PO4 solutions into the fibrin gel was performed at various pH conditions and fluoride concentrations to generate organic/inorganic composite materials mimicking biomineralization. The minerals produced in this system had a higher crystallinity than those generated by the Solution mineralization system. The minerals generated in fibrin gel varied depending on the pH conditions as follows: Dicalcium phosphate dihydrate (DCPD) in the noncontrolled pH solution, the DCPD and octacalcium phosphate (OCP) mixture at pH 7.4, and the OCP and hydroxyapatite (HAp) mixture at pH 9.0. When fluoride ions were added in the ram-e of 2-500 ppm, the minerals produced at pH 7.4 altered from OCP/HAp to HAp/fluorapatite (FAp). In addition, the crystallinity of the obtained minerals increased with an increase in fluoride ion concentration, and the Solubility was inversely correlated to crystallinity. In Conclusion, we established I novel fabrication method for synthesizing organic/inorganic composite materials composed of fibrin and calcium phosphate and revealed that the characteristics of the minerals in the synthesized material can be controlled by the fabrication condition. (C) 2007 Wiley Periodicals, Inc.

DOI： 10.1002/jbm.a.31777

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Functional control of myoblasts in a strained fibrin gel

T. Matsumoto, J. Sasaki, M. H. Uddin, H. Egusa, T. Sohmura

TISSUE ENGINEERING PART A 14 ( 5 ) 737 - 737 2008年5月

P-5 機械的刺激が骨芽細胞のHSCニッチ関連遺伝子発現に及ぼす影響(研究奨励賞応募ポスター発表,第51回日本歯科理工学会学術講演会)

佐々木淳一, 松本卓也, 江草宏, 荘村泰治, 矢谷博文

歯科材料・器械 27 ( 2 ) 140 - 140 2008年3月

An analysis of the persistent presence of opportunistic pathogens on patient-derived dental impressions and gypsum casts

Hiroshi Egusa, Takao Watamoto, Keiko Abe, Munemasa Kobayashi, Yoshitoshi Kaneda, Shunji Ashicla, Takuya Matsumoto, Hirofumi Yatani

INTERNATIONAL JOURNAL OF PROSTHODONTICS 21 ( 1 ) 62 - 68 2008年1月

記述言語：英語出版者・発行元：QUINTESSENCE PUBLISHING CO INC

Purpose: This study aimed to assess the persistent presence of microorganisms on patient-derived dental impressions and gypsum casts, while highlighting important human pathogens such as Candida, methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa. Materials and Methods: The practices and opinions regarding cross-infection control from 59 general dentists in Japan were obtained via a questionnaire. Alginate impressions were made from 56 patients. Using a brain heart infusion agar medium, impression and imprint cultures were carried out to visualize the microbial contamination on the surfaces of the impressions and gypsum casts, respectively. The colonies on the surfaces of the 30 impression cultures and 26 imprint cultures were collected by swabbing and then inoculated onto selective agar plates to detect streptococci, staphylococci, Candida, MRSA, and P aeruginosa. Results: The questionnaire showed that only 54% of general dentists had a cross-infection policy in their dental clinics, and only 30% to 40% were aware of the possible persistence of MRSA or P aeruginosa on impressions and gypsum casts. The impression/imprint cultures grew a large number of visible bacterial colonies on all of the impression/gypsum cast samples investigated. Selective agar cultures demonstrated the presence of streptococci (100, 100%), staphylococci (56.7, 65.4%), Candida (30, 46.2%), MRSA (26.7, 15.4%), and P aeruginosa (6.7, 7.7%) on the impressions and the gypsum casts, respectively. Conclusions: This investigation showed that patient-derived dental impressions and gypsum casts are contaminated with numerous microbes, including Candida, MRSA, and P aeruginosa, which are known pathogens responsible for nosocomial and/or life-threatening infection in the immunocompromised host.

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P10.三次元的配向骨基質の構築(一般演題抄録,第5回日本再生歯科学会)

佐々木淳一, 松本卓也, 江草宏, 荘村泰治, 矢谷博文

日本再生歯科医学会誌 5 ( 1 ) 58 - 58 2007年12月

Three-Dimensional Cell and Tissue Patterning in a Strained Fibrin Gel System

Takuya Matsumoto, Jun-Ichi Sasaki, Eben Alsberg, Hiroshi Egusa, Hirofumi Yatani, Taiji Sohmura

PLOS ONE 2 ( 11 ) e1211 2007年11月

記述言語：英語出版者・発行元：PUBLIC LIBRARY SCIENCE

Techniques developed for the in vitro reproduction of three-dimensional (3D) biomimetic tissue will be valuable for investigating changes in cell function in tissues and for fabricating cell/matrix composites for applications in tissue engineering techniques. In this study, we show that the simple application of a continuous strain to a fibrin gel facilitates the development of fibril alignment and bundle-like structures in the fibrin gel in the direction of the applied strain. Myoblasts cultured in this gel also exhibited well-aligned cell patterning in a direction parallel to the direction of the strain. Interestingly, the direction of cell proliferation was identical to that of cell alignment. Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cell patterning. In addition, vein endothelial cells formed a linear, aligned vessel-like structure in this system. Thus, the system enables the in vitro reproduction of 3D aligned cell sets replicating biological tissue patterns.

DOI： 10.1371/journal.pone.0001211

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Engineering tumors with 3D scaffolds

Claudia Fischbach, Ruth Chen, Takuya Matsumoto, Tobias Schmelzle, Joan S. Brugge, Peter J. Polverini, David J. Mooney

NATURE METHODS 4 ( 10 ) 855 - 860 2007年10月

記述言語：英語出版者・発行元：NATURE PUBLISHING GROUP

Microenvironmental conditions control tumorigenesis and biomimetic culture systems that allow for in vitro and in vivo tumor modeling may greatly aid studies of cancer cells' dependency on these conditions. We engineered three-dimensional ( 3D) human tumor models using carcinoma cells in polymeric scaffolds that recreated microenvironmental characteristics representative of tumors in vivo. Strikingly, the angiogenic characteristics of tumor cells were dramatically altered upon 3D culture within this system, and corresponded much more closely to tumors formed in vivo. Cells in this model were also less sensitive to chemotherapy and yielded tumors with enhanced malignant potential. We assessed the broad relevance of these findings with 3D culture of other tumor cell lines in this same model, comparison with standard 3D Matrigel culture and in vivo experiments. This new biomimetic model may provide a broadly applicable 3D culture system to study the effect of microenvironmental conditions on tumor malignancy in vitro and in vivo.

DOI： 10.1038/NMETH1085

Engineering tumors with 3D scaffolds

Claudia Fischbach, Ruth Chen, Takuya Matsumoto, Tobias Schmelzle, Joan S. Brugge, Peter J. Polverini, David J. Mooney

NATURE METHODS 4 ( 10 ) 855 - 860 2007年10月

記述言語：英語出版者・発行元：NATURE PUBLISHING GROUP

DOI： 10.1038/NMETH1085

松本卓也, 佐々木淳一, 荘村泰治

歯科材料・器械 26 ( 2 ) 85 - 85 2007年4月

Mechanical strain regulates endothelial cell patterning in vitro

Takuya Matsumoto, Yu Ching Yung, Claudia Fischbach, Hyun Joon Kong, Ryusuke Nakaoka, David J. Mooney

TISSUE ENGINEERING 13 ( 1 ) 207 - 217 2007年1月

記述言語：英語出版者・発行元：MARY ANN LIEBERT INC

Blood vessels of the vertebrate circulatory system typically exhibit tissue-specific patterning. However, the cues that guide the development of these patterns remain unclear. We investigated the effect of cyclic uniaxial strain on vascular endothelial cell dynamics and sprout formation in vitro in two-dimensional (2D) and three-dimensional (3D) culture systems under the influence of growth factors. Cells preferentially aligned and moved in the direction perpendicular to the major strain axis in monolayer culture, and mechanical strain also regulated the spatial location of cell proliferation in 2D cell culture. Cells in 3D cell culture could be induced to form sprouts by exposure to appropriate growth factor combinations (vascular endothelial growth factor and hepatocyte growth factor), and the strain direction regulated the directionality of this process. Moreover, cyclic uniaxial strain inhibited branching of the structures formed by endothelial cells and increased their thickness. Taken together, these data support the importance of external mechanical stimulation in the regulation of endothelial cell migration, proliferation, and differentiation into primitive vessels.

DOI： 10.1089/ten.2006.0058

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Modification of apatite materials for bone tissue engineering and drug delivery carriers

T. Matsumoto, M. Okazaki, A. Nakahira, J. Sasaki, H. Egusa, T. Sohmura

CURRENT MEDICINAL CHEMISTRY 14 ( 25 ) 2726 - 2733 2007年

記述言語：英語掲載種別：書評論文，書評，文献紹介等出版者・発行元：BENTHAM SCIENCE PUBL LTD

Apatite-related calcium phosphate, the main component of biological hard tissue, has good biocompatibility and is an economical material. Methods for the synthesis of apatite materials including hydroxyapatite (HAp) have previously been established. Therefore, for many years, apatite materials have been utilized as substitute materials for bone in orthopedic and dental fields. Such types of conventional substitute materials, which are implanted in the human body, should ostensibly be chemically stable to maintain their quality over time. However, recent advances in tissue engineering have altered this concept. Physicians and researchers now seek to identify materials that alter their properties temporally and spatially to achieve ideal tissue regeneration. In order to use apatite materials for tissue engineering and as drug delivery systems, the materials require both a high affinity for cells, tissues and/or functional molecules (e.g. growth factors and genes) and controllable bioabsorbability. To achieve these properties, various physicochemical modifications of apalite materials have been attempted. In addition, fabrication desiring three-dimensional structures (e.g. size, morphology and porositv) of apatite materials for implant sites could be one of the crucial techniques used to obtain ideal prognoses. In this review, the latest research trends relating to the techniques for the fabrication and modification of apatite materials are introduced.

DOI： 10.2174/092986707782023208

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フィブリンゲルを用いた in vitro 石灰化実験

楊類, 松本卓也, 佐々木淳一, 荘村泰治

歯科材料・器械 25 ( 5 ) 354 - 354 2006年9月

配向ハイドロゲル中での筋芽細胞分化制御

松本卓也, 佐々木淳一, UDIN M. H., 濱田吉之輔, 荘村泰治

歯科材料・器械 25 ( 5 ) 414 - 414 2006年9月

配向ハイドロゲル内での骨芽細胞様細胞分化に関する検討

佐々木淳一, 松本卓也, 江草宏, 荘村泰治, 矢谷博文

歯科材料・器械 25 ( 5 ) 315 - 315 2006年9月

配向ハイドロゲルを用いた骨芽細胞様細胞の三次元培養

佐々木淳一, 松本卓也, 江草宏, 矢谷博文

歯科材料・器械 25 ( 2 ) 183 - 183 2006年4月

高度配向ハイドロゲルを利用した筋芽細胞配向制御

松本卓也, 佐々木淳一, 濱田吉之輔

歯科材料・器械 25 ( 2 ) 89 - 89 2006年4月

機械的刺激の応用による細胞動態制御(細胞, 平成17年度秋期 (長崎) 第46回日本歯科理工学会学術講演会)

松本卓也

歯科材料・器械 24 ( 5 ) 341 - 341 2005年9月

Non-viral gene delivery regulated by stiffness of cell adhesion substrates

HJ Kong, JD Liu, K Riddle, T Matsumoto, K Leach, DJ Mooney

NATURE MATERIALS 4 ( 6 ) 460 - 464 2005年6月

記述言語：英語出版者・発行元：NATURE PUBLISHING GROUP

Non-viral gene vectors are commonly used for gene therapy(1-3) owing to safety concerns with viral vectors(4). However, nonviral vectors are plagued by low levels of gene transfection and cellular expression(1,2). Current efforts to improve the efficiency of non-viral gene delivery are focused on manipulations of the delivery vector(5-12), whereas the influence of the cellular environment in DNA uptake is oft en ignored. The mechanical properties ( for example, rigidity) of the substrate to which a cell adheres have been found to mediate many aspects of cell function including proliferation, migration and differentiation(13-17), and this suggests that the mechanics of the adhesion substrate may regulate a cell's ability to uptake exogeneous signalling molecules. In this report, we present a critical role for the rigidity of the cell adhesion substrate on the level of gene transfer and expression. The mechanism relates to material control over cell proliferation, and was investigated using a fluorescent resonance energy transfer (FRET) technique(18-21). This study provides a new material-based control point for non-viral gene therapy.

DOI： 10.1038/nmat1392

濱田吉之輔, 結城加奈子, 小上典久, 松本卓也, 高橋純造, 松浦成昭

歯科材料・器械 24 ( 2 ) 134 - 134 2005年4月

Hydroxyapatite particles as a controlled release carrier of protein

T Matsumoto, M Okazaki, M Inoue, S Yamaguchi, T Kusunose, T Toyonaga, Y Hamada, J Takahashi

BIOMATERIALS 25 ( 17 ) 3807 - 3812 2004年8月

記述言語：英語出版者・発行元：ELSEVIER SCI LTD

This study examines the possibility of using hydroxyapatite (HAp) particles as a controlled release carrier of protein. In order to achieve effective protein release from HAp particles, it is necessary to regulate the conjugated amount of protein on HAp and the resorption of HAp. HAp particles were synthesized at different temperatures (40 degreesC, 60 degreesC, 80 degreesC) in wet condition and the physicochemical properties of synthesized HAp particles were examined. HAp particles synthesized at low temperatures showed low crystallinity, high solubility and large specific surface area. The useful growth factors for bone regeneration, such as BMP, bFGF and TGF-beta, are basic proteins, so cytochrome c (pI = 10.2) was used as a model protein and the adsorptive property of protein on HAp particles was investigated. The protein adsorption on HAp particles changed depending on its specific surface area and the chart of protein adsorption on HAp particles showed a typical Langmuir curve. These findings suggest that the adsorbed amount of protein on HAp particles could be regulated by HAp synthesizing temperature and the concentrations of protein solution. The release kinetics of protein from the HAp particles that adsorbed the protein (HAp-pro) was also evaluated in different pH solutions (pH 4.0 and 7.0). The released protein gradually increased time dependently when HAp-pro were immersed in pH 4.0 solution, but the released protein was significantly smaller when HAp-pro were immersed in pH 7.0 solution. Moreover, the release rate of protein from HAp-pro differed in each HAp that was synthesized at different temperatures, suggesting that the release of protein from HAp-pro depended on HAp resorption. These results suggest that HAp particles synthesized at different temperature are useful as a controlled release carrier of protein. (C) 2003 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biomaterials.2003.10.081

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