研究者詳細 - 松本　卓也

MISC - 松本　卓也

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P-2 機能性ペプチド修飾ゲルを用いた唾液腺の成長制御(研究奨励賞応募ポスター発表,第62回日本歯科理工学会学術講演会)

武田宏明, シャティグルサンアラ, ファラハトマハムド, 鳥井康弘, 松本卓也

日本歯科理工学会誌 32 ( 5 ) 336 - 336 2013年9月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

In vitro fabrication of biological tissue from cells is one of the biggest challenges in bioengineering. For this purpose, the growth and morphological control of tissue is crucial. To control the submandibular gland tissue (SMG) morphogenesis, RGD immobilized alginate hydrogel was prepared, and used for its organotypic culture in this study. RGD immobilized hydrogel sheet enhanced the SMG branching. Moreover, the RGD immobilized hydrogel bead enhanced the region-specific cleft formation in the cultured SMG. Thus, the chemically-modified biomimetic environment fabricated by hydrogel material would be a valuable tool for in vitro modulation of the SMG tissue growth and morphology.

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岡山大学大学院医歯薬学総合研究科生体材料学分野

松本卓也

接着歯学 = Adhesive dentistry 30 ( 1 ) 52 - 52 2012年4月

ワンステップボンディングシステムにおける活性ラジカル発生挙動と接着性 : 光重合開始剤の配合量による変化

武田宏明, 鳥井康弘, 松本卓也, 鈴木一臣

日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices 31 ( 2 ) 183 - 183 2012年3月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

This study investigated the relationship between concentration of photoinitiator and generating behavior of polymer radicals, degree of conversion, and bond strength to tooth substrates in one-step adhesive. The experimental adhesives contained Bis-GMA, HEMA, MDP, ethanol, water and various concentration (1, 3 and 5 wt%) of Camphorquinone and Ethyl p-(dimethylamino)benzoate as initiators. The adhesive containing 3wt% initiators always generated higher amount of polymer radicals as compared with the others which showed similar generating behavior. The adhesive containing 1wt% initiators showed the lowest value in degree of conversion and bond strength, while the other two showed relatively high values.

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細胞集合体を用いた内軟骨性骨化 in vitro 定量解析ツールの開発

佐々木淳一, 松本卓也, 江草宏, 矢谷博文, 今里聡

日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices 31 ( 2 ) 138 - 138 2012年3月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

We previously reported that three-dimensional (3D) mesenchymal stem cells (MSCs) construct could be fabricated by using thermo-responsive hydrogel. In this study, internal/external environments of the 3D cell construct induced by osteoblastic differentiation were investigated. The specific regions with chondrogenesis and osteogenesis were detected in the 3D cell construct during the culture. Calcified matrix was formed from MSCs inthe process of endochondral ossification. The 3D cell construct composed of MSCs could be a promising toolfor in vitro investigation of osteogenesis.

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ハイドロゲルを応用した三次元配向組織の in vitro 構築

佐々木淳一, 松本卓也, 江草宏, 矢谷博文

大阪大學齒學雜誌 = The Journal of Osaka University Dental Society 55 ( 2 ) 83 - 89 2011年4月

第56回日本歯科理工学会学術講演会

松本卓也

バイオマテリアル : 生体材料 : Journal of Japanese Society for Biomaterials 29 ( 1 ) 56 - 57 2011年2月

スキャフォールドフリー三次元細胞構造体の作製

松本卓也, 佐々木淳一, 江草宏, 矢谷博文

日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices 29 ( 5 ) 382 - 382 2010年9月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

In tissue regeneration therapy, scaffold materials play pivotal roles on carrying cells at the site of tissue regeneration. However, the remained scaffold materials might attenuate the accurate tissue regeneration in some cases. Therefore, creating another cell carrying method without using scaffold material would be important. To obtain scaffold free-3D cell constructs, we synthesized thermo-responsive hydrogel by using poly N-isopropylacrylamide with several cross-linking agents. Consequently, we fabricated clear hydrogel, which changes its volume depending on the temperature. The gel allowed us to obtain 3D cell constructs with desired morphology.

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Apatite Containing Aspartic Acid for Selective Protein Loading

M. Hafiz Uddin, T. Matsumoto, S. Ishihara, A. Nakahira, M. Okazaki, T. Sohmura

JOURNAL OF DENTAL RESEARCH 89 ( 5 ) 488 - 492 2010年5月

記述言語：英語出版者・発行元：SAGE PUBLICATIONS INC

Physico-chemical modifications of hydroxyapatite (HAp) materials are considered as pre-requisites for the development of new bioactive carrier materials for drug delivery and tissue engineering applications. Since acidic amino acids have well-documented affinities to both HAp and basic proteins, HAp modified by aspartic acid (Asp, acidic amino acid) might be one of the candidate substrates for a basic protein carrier. Here, we synthesized HAp in the presence of various concentrations of Asp and observed that HAp crystallinity and other physico-chemical properties were effectively modulated. Detailed studies indicated that Asp was not incorporated in the HAp crystal lattice, but rather was trapped in HAp crystals. Protein adsorption studies indicated that the HAp particles modified by Asp had a selective loading capacity for basic protein. Therefore, HAp particles containing Asp might have potential in drug delivery applications, especially as the carrier of basic proteins including bFGF and BMP.

DOI： 10.1177/0022034509357309

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異なる配合比を有するZrO_2/HAp複合体の生物学的性質に関する検討

松本卓也, 荘村泰治

日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices 29 ( 2 ) 118 - 118 2010年3月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

To obtain bioceramic that has both sufficient strength and biocompatibility for bone reconstruction, ZrO_2 /HAp composite with different ratio was sintered at 1500℃. SEM images indicated that ZrO_2 (100%) had a solid structure, whereas sintered HAp (100%) remained porous structure. Protein adsorption, cell adhesion and animal experiments indicated the drastic improvement of bioactivity of materials by containing HAp.

松本卓也, UDDIN Mohammad Hafiz, アンサンヒュン, 荘村泰治

日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices 29 ( 2 ) 142 - 142 2010年3月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

Our recent study indicated that hydroxyapatite (HAp) synthesized in the presence of aspartic acid showed special affinity to basic molecules. In addition, the detailed TEM observations indicated that small peapod like voids were formed in the HAp-Asp crystals. To understand the mechanism of this void formation, further TEM observations of synthesized HAp, HAp-Gly or HAp-Asp synthesized with different concentration of Asp were carried out. The results indicated that the organics contained in HAp-Asp or HAp-Gly were crucial on this void formation and alignment.

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Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification

Shiho Ishihara, Takuya Matsumoto, Takamasa Onoki, Mohammad Hafiz Uddin, Taiji Sohmura, Atsushi Nakahira

ACTA BIOMATERIALIA 6 ( 3 ) 830 - 835 2010年3月

記述言語：英語出版者・発行元：ELSEVIER SCI LTD

Loading and releasing protein in a controllable way is extremely important for the protein vehicles used in bone tissue engineering. To obtain a suitable carrier material for basic proteins, such as BMP or bFGF, hydroxyapatite particles containing mercaptosuccinic acid (mercaptosuccinic acid (Met), (Mer-HAp)) were synthesized Physicochemical evaluation of Mer-HAp suggested that Mer was contained in HAp particles: it either simply adsorbed onto HAp crystals or was trapped among the HAp crystals. A protein adsorption Study using basic and acidic model proteins indicated that the synthesized Mer-HAp had selective loading properties of the basic protein. The loaded protein was gradually released from Mer-HAp in phosphate buffered saline. The protein release rate was different in each Mer-HAp synthesized with a different concentration of Mer. In addition, the protein release from Mer-HAp showed a similar profile with the Ca dissolution in different pH solutions, indicating that the Mer-HAp dissolution was concerned with the protein release from Mer-HAp Thus, Mer-HAp is a useful candidate for the basic protein carrier because it has properties which enable the loading and releasing of protein in a controllable way. (C) 2009 Acta Materialia Inc Published by Elsevier Ltd. All rights reserved

DOI： 10.1016/j.actbio.2009.10.019

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物理的刺激を応用した三次元ハイドロゲル内での細胞操作

松本卓也

バイオマテリアル : 生体材料 : Journal of Japanese Society for Biomaterials 28 ( 1 ) 46 - 53 2010年2月

Mass Fabrication of Small Cell Spheroids by Using Micro-patterned Tissue Culture Plate

Akinari Iwasaki, Takuya Matsumoto, Go Tazaki, Hitoshi Tsuruta, Hiroshi Egusa, Hiroyuki Miyajima, Taiji Sohmura

ADVANCED ENGINEERING MATERIALS 11 ( 10 ) 801 - 804 2009年10月

唾液腺分岐形態形成制御へ向けたゲル材料の応用

宮嶋宏行, 松本卓也, 荘村泰治

歯科材料・器械 28 ( 5 ) 226 - 226 2009年9月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

Branching morphogenesis is an essential morphological process observed in developmental glandular organs. For organ culture substrate, alginate hydrogel with different mechanical property (stiffness) or chemical property (modified by different concentration of RGD peptide) was prepared. Embryonic mouse submandibular glands (SMG) cultured on the soft substrate or on the substrate modified by high concentration of RGD peptide enhanced the branching morphogenesis of SMG. Thus, the physico-chemical modification of tissue culture substrate would be one of the valuable methods that one can control the branching morphogenesis of salivary gland development.

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異なる配合比を有するHAp/ZrO_2複合体の特性に関する検討

松本卓也, 荘村泰治, 荘村泰治

歯科材料・器械 28 ( 5 ) 265 - 265 2009年9月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

To obtain bioceramic that has both sufficient strength and biocompatibility for bone reconstruction, HAp and ZrO_2 with different ratio was sintered at 1500℃. SEM images indicated that sintered HAp (100%) remained porous structure, whereas ZrO_2 (100%) had a solid structure. The composite ceramics (HAp/ZrO_2=40/60) showed lowest contraction (22.55%) in volume. Thus, the composite ceramics have different material properties depending on the mixture ratio of HAp and ZrO_2.

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ZrO_2/HAp複合材料の in vivo 評価

安相〓, 松本卓也, 宮嶋宏行, 中平敦, 荘村泰治

歯科材料・器械 28 ( 5 ) 275 - 275 2009年9月

記述言語：日本語出版者・発行元：一般社団法人日本歯科理工学会

Fibrin is a natural polymer known for supporting wound healing by inducing angiogenesis and promoting cell attachment and proliferation. Thus fibrin may provide an ideal environment for accelerating bone regeneration. In this study, we developed a novel composite scaffold consist of ZrO_2/HAp and fibrin gel with osteoblasts and evaluated the biocompatibility of the synthesized materials.

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Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts

Hiroshi Egusa, Yoshitoshi Kaneda, Yoshihiro Akashi, Yoshinosuke Hamada, Takuya Matsumoto, Makio Saeki, Devang K. Thakor, Yasuhiko Tabata, Nariaki Matsuura, Hirofumi Yatani

BIOMATERIALS 30 ( 27 ) 4676 - 4686 2009年9月

記述言語：英語出版者・発行元：ELSEVIER SCI LTD

Recently, the binding sequence Ser-Val-Val-Tyr-Gly-Leu-Arg (SVVYGLR) was found adjacent to the RGD sequence in osteopontin, suggesting involvement in osteo-immune cross-talk. The aim of this study was to investigate bioactive functions of a synthetic SVVYGLR peptide in osteoprogenitor cells and osteoclasts, and to examine potential applications in bone regeneration. The SVVYGLR peptide significantly enhanced the adhesion and proliferation of several human mesenchymal cells including bone marrow-derived mesenchymal stem cells, and alpha v beta 3 integrin was involved in cell attachment to the peptide. Additionally, the peptide reduced the number of TRAP-positive multinucleated cells during osteoclastogenesis of RAW264.7 cells and normal murine pre-osteoclasts, and also suppressed NFAT activity and expression of osteoclastogenesis-related mRNAs. When standardized bone defects in rat calvariae were filled with a collagen sponge containing the peptide or PBS (control), the number of TRAP-positive osteoclasts in the grafted sites after 3 weeks was significantly lower in the peptide group. By the 5th week, significantly enhanced resorption of the grafted collagen sponge and new bone formation was observed within and surrounding the sponge in the peptide group. These data suggest that SVVYGLR is an effective bioactive peptide for bone tissue regeneration that promotes attachment and proliferation of osteogenic cells while also suppressing osteoclastogenesis. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biomaterials.2009.05.032

New concept bioceramics composed of octacalcium phosphate (OCP) and dicarboxylic acid-intercalated OCP via hydrothermal hot-pressing

Shiho Ishihara, Takuya Matsumoto, Takamasa Onoki, Taiji Sohmura, Atsushi Nakahira

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 29 ( 6 ) 1885 - 1888 2009年8月

記述言語：英語出版者・発行元：ELSEVIER SCIENCE BV

Octacalcium phosphate (OCP) and adipic acid-intercalated complexed OCP (Adi-OCP) were synthesized. Moreover, we made ceramic bodies out of them through a hydrothermal hot-pressing (HHP) method. Characteristic features of both the powder and ceramics were investigated by the X-ray diffraction method (XRD). Surface morphology of the ceramics was observed by scanning electron microscopy (SEM). Density, compressive strength and pore size distribution of the ceramics were measured. Crystalline structure of the newly developed OCP ceramics had no phase transformation from the starting materials. Moreover, the newly developed OCP ceramics had good mechanical properties only through the HHP treatment with a temperature as low as 110 degrees C. In order to evaluate bioactivity, the ceramics were immersed in simulating body fluid (SBF). It was predicted that OCP and Adi-OCP had better bioactivity than that of conventional HAp ceramics. (C) 2009 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.msec.2009.02.023

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ZrO_2/HA複合材料の作製及び細胞との親和性に関する研究

安相〓, 松本卓也, 石原四穂, 中平敦, 荘村泰治

歯科材料・器械 28 ( 2 ) 110 - 110 2009年3月

異なる弾性率を有するアルジネートゲル上でのマウス唾液腺の分岐形態形成

宮嶋宏行, 松本卓也, 安相〓, 荘村泰治

歯科材料・器械 28 ( 2 ) 77 - 77 2009年3月

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