研究者詳細 - 中村　宜督

論文 - 中村　宜督

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Difference in Abscisic Acid Perception Mechanisms between Closure Induction and Opening Inhibition of Stomata 査読

Ye Yin, Yuji Adachi, Wenxiu Ye, Maki Hayashi, Yoshimasa Nakamura, Toshinori Kinoshita, Izumi C. Mori, Yoshiyuki Murata

PLANT PHYSIOLOGY 163 ( 2 ) 600 - 610 2013年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC PLANT BIOLOGISTS

Abscisic acid (ABA) induces stomatal closure and inhibits light-induced stomatal opening. The mechanisms in these two processes are not necessarily the same. It has been postulated that the ABA receptors involved in opening inhibition are different from those involved in closure induction. Here, we provide evidence that four recently identified ABA receptors (PYRABACTIN RESISTANCE1 [PYR1], PYRABACTIN RESISTANCE-LIKE1 [PYL1], PYL2, and PYL4) are not sufficient for opening inhibition in Arabidopsis (Arabidopsis thaliana). ABA-induced stomatal closure was impaired in the pyr1/pyl1/pyl2/pyl4 quadruple ABA receptor mutant. ABA inhibition of the opening of the mutant's stomata remained intact. ABA did not induce either the production of reactive oxygen species and nitric oxide or the alkalization of the cytosol in the quadruple mutant, in accordance with the closure phenotype. Whole cell patch-clamp analysis of inward-rectifying K+ current in guard cells showed a partial inhibition by ABA, indicating that the ABA sensitivity of the mutant was not fully impaired. ABA substantially inhibited blue light-induced phosphorylation of H+-ATPase in guard cells in both the mutant and the wild type. On the other hand, in a knockout mutant of the SNF1-related protein kinase, srk2e, stomatal opening and closure, reactive oxygen species and nitric oxide production, cytosolic alkalization, inward-rectifying K+ current inactivation, and H+-ATPase phosphorylation were not sensitive to ABA.

DOI： 10.1104/pp.113.223826

Endogenous abscisic acid is involved in methyl jasmonate-induced reactive oxygen species and nitric oxide production but not in cytosolic alkalization in Arabidopsis guard cells 査読

Wenxiu Ye, Mohammad Anowar Hossain, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

JOURNAL OF PLANT PHYSIOLOGY 170 ( 13 ) 1212 - 1215 2013年9月

記述言語：英語出版者・発行元：ELSEVIER GMBH, URBAN & FISCHER VERLAG

We recently demonstrated that endogenous abscisic acid (ABA) is involved in methyl jasmonate (MeJA)induced stomatal closure in Arabidopsis thaliana. In this study, we investigated whether endogenous ABA is involved in MeJA-induced reactive oxygen species (ROS) and nitric oxide (NO) production and cytosolic alkalization in guard cells using an ABA-deficient Arabidopsis mutant, aba2-2, and an inhibitor of ABA biosynthesis, fiuridon (FLU). The aba2-2 mutation impaired MeJA-induced ROS and NO production. FLU inhibited MeJA-induced ROS production in wild-type guard cells. Pretreatment with 0.1 mu M ABA, which does not induce stomatal closure in the wild type, complemented the insensitivity to MeJA of the aba2-2 mutant. However, MeJA induced cytosolic alkalization in both wild-type and aba2-2 guard cells. These results suggest that endogenous ABA is involved in MeJA-induced ROS and NO production but not in MeJA-induced cytosolic alkalization in Arabidopsis guard cells. (C) 2013 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.jplph.2013.03.011

Catalases CAT1 and CAT3 Are not Key Enzymes in Alleviating Gamma Irradiation-Induced DNA Damage, H2O2 Accumulation, or Lipid Peroxidation in Arabidopsis thaliana 査読

Amena Sultana, Ikuko Minami, Daiki Matsushima, Mohammad Issak, Yoshimasa Nakamura, Setsuko Todoriki, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 9 ) 1984 - 1987 2013年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

Gamma irradiation increased catalase activities at 0.1 kGy and decreased them at 10 kGy in Arabidopsis wild type and catalase-deficient mutants, cat3-1 and cat1 cat3. Irradiation induced DNA damage, H2O2 accumulation, and lipid peroxidation in both mutants as well as the wild type. Thus catalases might not be key enzymes protecting gamma irradiation-induced damage.

DOI： 10.1271/bbb.130336

Lower Photostability of Capsanthin Dispersed in an Aqueous Solution 査読

Masayuki Nishino, Makoto Sakata, Yoshiyuki Murata, Yoshimasa Nakamura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 6 ) 1313 - 1316 2013年6月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

Food-additive grades of capsanthin, lutein, lycopene, and beta-carotene dispersed in aqueous solutions were photo-irradiated using a Xenon weather meter, and the levels of carotenoids were measured by HPLC and the absorbance method. Capsanthin photo-degraded more rapidly than the carotenoids tested, with less oxygen consumption. Unlike carotenes, capsanthin was partially converted into analogous colored compounds during degradation.

DOI： 10.1271/bbb.130033

Neither Endogenous Abscisic Acid nor Endogenous Jasmonate Is Involved in Salicylic Acid-, Yeast Elicitor-, or Chitosan-Induced Stomatal Closure in Arabidopsis thaliana 査読

Mohammad Issak, Eiji Okuma, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 5 ) 1111 - 1113 2013年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

Salicylic acid (SA), yeast elicitor (YEL), and chitosan (CHT) induced stomatal closure in Arabidopsis wildtype and aba2-2 plants, induced stomatal closure in fluridon-treated wild-type plants, and induced stomatal closure in aos mutants. These results suggest that neither endogenous abscisic acid nor endogenous jasmonic acid is involved in SA-, YEL-, or CHT-induced stomatal closure.

DOI： 10.1271/bbb.120980

Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells 査読

M. A. Salam, F. Jammes, M. A. Hossain, W. Ye, Y. Nakamura, I. C. Mori, J. M. Kwak, Y. Murata

Plant Biology 15 ( 3 ) 436 - 442 2013年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

We report that two mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate abscisic acid (ABA)-induced stomatal closure in Arabidopsis thaliana. Yeast elicitor (YEL) induced stomatal closure accompanied by intracellular reactive oxygen species (ROS) accumulation and cytosolic free calcium concentration ([Ca2+]cyt) oscillation. In this study, we examined whether these two MAP kinases are involved in YEL-induced stomatal closure using MAPKK inhibitors, PD98059 and U0126, and MAPK mutants, mpk9, mpk12 and mpk9 mpk12. Both PD98059 and U0126 inhibited YEL-induced stomatal closure. YEL induced stomatal closure in the mpk9 and mpk12 mutants but not in the mpk9 mpk12 mutant, suggesting that a MAPK cascade involving MPK9 and MPK12 functions in guard cell YEL signalling. However, YEL induced extracellular ROS production, intracellular ROS accumulation and cytosolic alkalisation in the mpk9, mpk12 and mpk9 mpk12 mutants. YEL induced [Ca2+]cyt oscillations in both wild type and mpk9 mpk12 mutant. These results suggest that MPK9 and MPK12 function redundantly downstream of extracellular ROS production, intracellular ROS accumulation, cytosolic alkalisation and [Ca2+]cyt oscillation in YEL-induced stomatal closure in Arabidopsis guard cells and are shared with ABA signalling. © 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.

DOI： 10.1111/j.1438-8677.2012.00671.x

Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis 査読

Nasima Akter, Eiji Okuma, Muhammad Abdus Sobahan, Misugi Uraji, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

JOURNAL OF PLANT GROWTH REGULATION 32 ( 1 ) 208 - 215 2013年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER

Glutathione (GSH) has been shown to negatively regulate methyl jasmonate (MeJA)-induced stomatal closure. We investigated the roles of GSH in MeJA signaling in guard cells using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, gamma-glutamylcysteine synthetase. MeJA-induced stomatal closure and decreased GSH contents in guard cells. Decreasing GSH by the cad2-1 mutation enhanced MeJA-induced stomatal closure. Depletion of GSH by the cad2-1 mutation or increment of GSH by GSH monoethyl ester did not affect either MeJA-induced production of reactive oxygen species (ROS) or MeJA-induced cytosolic alkalization in guard cells. MeJA and abscisic acid (ABA) induced stomatal closure and GSH depletion in atrbohD and atrbohF single mutants but not in the atrbohD atrbohF double mutant. Moreover, exogenous hydrogen peroxide induced stomatal closure but did not deplete GSH in guard cells. These results indicate that GSH affects MeJA signaling as well as ABA signaling and that GSH negatively regulates a signal component other than ROS production and cytosolic alkalization in MeJA signal pathway of Arabidopsis guard cells.

DOI： 10.1007/s00344-012-9291-7

Effects of emulsifiers on the photostability of lycopene 査読

Masayuki Nishino, Makoto Sakata, Yoshiyuki Murata, Yoshimasa Nakamura

Food Science and Technology Research 19 ( 6 ) 983 - 987 2013年

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Aqueous solutions of dispersed lycopene in which gum arabic, gum ghatti and polyglycerol monostearate ester had been used as emulsifiers were photoirradiated. The photodegradation rate of lycopene tended to vary depending on the amount of polyglycerol monostearate ester used. We found that the photodegradation rate of lycopene with polyglycerol monostearate ester was considerably slower than those with gum arabic and gum ghatti. Therefore, this study suggests that the absorption state of the emulsifier on the crystal surface may affect the photodegradation rate of lycopene.

DOI： 10.3136/fstr.19.983

Arabidopsis thalianaにおけるガンマ線照射誘導過酸化水素蓄積と脂質過酸化の軽減にカタラーゼCAT2は関与しない査読

Amena Sultana, 南育子, 松島大貴, Mohammad Issak, 中村宜督, 等々力節子, 村田芳行

食品照射 48 ( 1 ) 38 - 41 2013年

記述言語：日本語出版者・発行元：日本食品照射研究協議会

シロイヌナズナ野生株（Col-0）及び<i>cat2</i>変異体への10 kGyのガンマ線照射は，H<sub>2</sub>O<sub>2</sub>蓄積及び脂質過酸化生成を誘起したが，0.1 kGyと1 kGyのガンマ線照射は，誘起しなかった。また，野生株と変異体との間で，H<sub>2</sub>O<sub>2</sub>蓄積及び脂質過酸化に有意な差はなかった。以上の結果より，カタラーゼCAT2は，ガンマ線によって誘起される損傷を軽減するための重要な酵素ではないことが示唆された。

DOI： 10.5986/jrafi.48.38

Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in arabidopsis thaliana 査読

Mohammad Shakhawat Hossain, Wenxiu Ye, Mohammad Anowar Hossain, Eiji Okuma, Misugi Uraji, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Bioscience, Biotechnology and Biochemistry 77 ( 5 ) 977 - 983 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）

Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca2+]cyt) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAMsensitive peroxidases, intracellular ROS accumulation, and [Ca 2+]cyt oscillation in Arabidopsis.

DOI： 10.1271/bbb.120928

Tribolium castaneumの幼虫と成虫へのガンマ線の影響査読

Amena Sultana, 南育子, 市場隆二, Mohammad Issak, 多田幹郎, 中村宜督, 宮竹貴久, 等々力節子, 村田芳行

食品照射 48 ( 1 ) 19 - 23 2013年

記述言語：日本語出版者・発行元：日本食品照射研究協議会

ガンマ線を用いた食品照射は，穀物やスパイスの害虫の侵入を抑制する手段の一つである。我々は，<i>Tribolium castaneum</i>の幼虫と成虫へのガンマ線照射の影響を調べた。羽化は，500Gyの照射で完全に阻害された。成虫の生存率は，500Gy以上の照射で著しく減少した。中性条件でのコメントアッセイの結果より，500Gyまたは1000Gy照射によるDNA損傷は，不可逆的に増加したが，100Gy照射によるDNA損傷は一過的に増加した。100Gy照射ではDNAの修復が行われていることが示唆された。以上の結果より500Gy以上のガンマ線照射は，<i>T. castaneum </i>の幼虫と成虫の駆除に十分であることが示された。

DOI： 10.5986/jrafi.48.19

Effects of Depletion of Glutathione on Abscisic Acid- and Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis thaliana 査読

Nasima Akter, Muhammad Abdus Sobahan, Misugi Uraji, Wenxiu Ye, Mohammad Anowar Hossain, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 76 ( 11 ) 2032 - 2037 2012年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

Glutathione (GSH) is involved in abscisic acid (ABA)-and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJA-induced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca2+ oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca2+ oscillation in guard cells of Arabidopsis.

DOI： 10.1271/bbb.120384

Development and application of a method for identification of isothiocyanate-targeted molecules in colon cancer cells. 査読国際誌

Noriyuki Miyoshi, Takumi Yonemochi, Susumu Tomono, Ryuuta Fukutomi, Yoshimasa Nakamura, Hiroshi Ohshima

Analytical biochemistry 429 ( 2 ) 124 - 31 2012年10月

記述言語：英語

In this study, we have developed a novel method to identify isothiocyanate (ITC)-targeted molecules using two well-studied ITCs: benzyl ITC (BITC) and phenethyl ITC (PEITC). The principle of this method is based on identifying a pattern of differences between BITC and PEITC given that they show similar chemical and biological behaviors. For method validation, dithiothreitol-reduced bovine insulin as a model molecule was incubated with either BITC or PEITC, and digested peptides were analyzed by ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF-MS) and liquid chromatography quadrupole TOF-MS (LC-Q-TOF-MS). Three peptides-NYCN, FVNQHLCGSHLVE, and ALYLVCGE-were identified as being adducted with BITC or PEITC on their cysteine residues. Each set of peptides adducted with either BITC or PEITC showed retention times (RT(BITC)<RT(PEITC)) by reverse-phase column chromatography with a difference of molecular mass (Δ14.01565). On the basis of these findings, computational mathematical schemes were constructed to extract sets of MS ions satisfying the above criteria. Application of the developed method to an extract of ITC-treated human colon cancer HCT116 cells, thiocarbamoylation of cysteine residues of glutathione, and the N-terminal proline residues of PMFIVNTNVPR from macrophage migration inhibitory factor were successfully identified as one of the intracellular targets of ITCs. Moreover, the method also detected the thiocarbamoylated conjugates of ITCs with intracellular free cysteines and lysines.

DOI： 10.1016/j.ab.2012.07.018

Methylglyoxal inhibits seed germination and root elongation and up-regulates transcription of stress-responsive genes in ABA-dependent pathway in Arabidopsis 査読

T. S. Hoque, M. Uraji, A. Tuya, Y. Nakamura, Y. Murata

PLANT BIOLOGY 14 ( 5 ) 854 - 858 2012年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY-BLACKWELL

Methylglyoxal (MG) is a highly reactive metabolite derived from glycolysis. In this study, we examined the effect of MG on seed germination, root elongation, chlorosis and stress-responsive gene expression in Arabidopsis using an abscisic acid (ABA)-deficient mutant, aba2-2. In the wild type, 0.1 mm MG did not affect germination but delayed root elongation, whereas 1.0 mm MG inhibited germination and root elongation and induced chlorosis. MG increased transcription levels of RD29B and RAB18 in a dose-dependent manner but did not affect RD29A transcription level. In contrast, in the aba2-2 mutant, MG inhibition of seed germination at 1.0 mm and 10.0 mm and a delay of root elongation at 0.1 mm MG were mitigated, although there was no significant difference in chlorosis between the wild type and mutant. Moreover, the aba2-2 mutation impaired MG-induced RD29B and RAB18 gene expression. These observations suggest that MG not only directly inhibits germination and root elongation but also indirectly modulates these processes via endogenous ABA in Arabidopsis.

DOI： 10.1111/j.1438-8677.2012.00607.x

MAP Kinases, MPK9 and MPK12, Regulate Chitosan-Induced Stomatal Closure 査読

Mohammad Abdus Salam, Fabien Jammes, Mohammad Anowar Hossain, Wenxiu Ye, Yoshimasa Nakamura, Izumi C. Mori, June M. Kwak, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 76 ( 9 ) 1785 - 1787 2012年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

Chitosan (CHT)-induced stomatal closure was inhibited by an MAPKK inhibitor, PD98059, and was impaired in mpk9 mpk12 but not in mpk9 or mpk12. CHT induced the production of reactive oxygen species, cytosolic alkalization, and cytosolic Ca2+ oscillation in mpk9 mpk12. These results suggest that MPK9 and MPK12 are involved in CHT-induced stomatal closure.

DOI： 10.1271/bbb.120228

Effects of Exogenous Proline and Glycinebetaine on the Salt Tolerance of Rice Cultivars 査読

Muhammad Abdus Sobahan, Nasima Akter, Misao Ohno, Eiji Okuma, Yoshihiko Hirai, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 76 ( 8 ) 1568 - 1570 2012年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：TAYLOR & FRANCIS LTD

Salinity significantly increased trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS) uptake and decreased the K+/Na+ ratio in salt-sensitive rice (Nipponbare) but did not markedly in salt-tolerant rice (Pokkali). Proline and glycinebetaine (betaine) suppressed the increase in PTS uptake and the decrease in the K+/Na+ ratio in Nipponbare, but did not affect PTS uptake or the K+/Na+ ratio in Pokkali.

DOI： 10.1271/bbb.120233

Methylglyoxal inhibition of cytosolic ascorbate peroxidase from Nicotiana tabacum 査読

Md. Anamul Hoque, Misugi Uraji, Akiko Torii, Mst. Nasrin Akhter Banu, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 26 ( 8 ) 315 - 321 2012年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY-BLACKWELL

Methylglyoxal (MG) is one of the aldehydes accumulated in plants under environmental stress. Cytosolic ascorbate peroxidase (cAPX) plays a key role in the protection of cells from oxidative damage by scavenging reactive oxygen species in higher plants. A cDNA encoding cAPX, named NtcAPX, was isolated from Nicotiana tabacum. We characterized recombinant NtcAPX (rNtcAPX) as a fusion protein with glutathione S-transferase to investigate the effects of MG on APX. NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa. The rNtcAPX showed a higher APX activity. MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing Km for ascorbate. On the contrary, neither NaCl nor cadmium reduced the activity of APX. The present study suggests that inhibition of APX is in part due to the modification of amino acids by MG. (c) 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:315321, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21423

DOI： 10.1002/jbt.21423

Hydogen peroxide-dependent photocytotoxicity by phloxine B, a xanthene-type food colorant (vol 1810, pg 704, 2011) 査読

Hang Qi, Hiroshi Takano, Yoji Kato, Qian Wu, Chiharu Ogata, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1820 ( 7 ) 1020 - 1020 2012年7月

Methylglyoxal-induced stomatal closure accompanied by peroxidase-mediated ROS production in Arabidopsis 査読

Tahsina Sharmin Hoque, Misugi Uraji, Wenxiu Ye, Mohammad Anowar Hossain, Yoshimasa Nakamura, Yoshiyuki Murata

JOURNAL OF PLANT PHYSIOLOGY 169 ( 10 ) 979 - 986 2012年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER GMBH, URBAN & FISCHER VERLAG

Methylglyoxal (MG) is an oxygenated short aldehyde and a glycolytic intermediate that accumulates in plants under environmental stresses. Being a reactive alpha-oxoaldehyde, MG may act as a signaling molecule in plants during stresses. We investigated whether MG induces stomatal closure, reactive oxygen species (ROS) production, and cytosolic free calcium concentration ([Ca2+](cyt)) to clarify roles of MG in Arabidopsis guard cells. MG induced production of ROS and [Ca2+](cyt) oscillations, leading to stomatal closure. The MG-induced stomatal closure and ROS production were completely inhibited by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), but were not affected by an NAD(P)H oxidase mutation, atrbohD atrbohF. Furthermore, the MG-elicited [Ca2+](cyt), oscillations were significantly suppressed by SHAM but not by the atrbohD atrbohF mutation. Neither endogenous abscisic acid nor endogenous methyl jasmonate was involved in MG-induced stomatal closure. These results suggest that intrinsic metabolite MG can induce stomatal closure in Arabidopsis accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca2+](cyt) oscillations. (C) 2012 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.jplph.2012.02.007

Catalases negatively regulate methyl jasrnonate signaling in guard cells 査読

Rayhanur Jannat, Misugi Uraji, Mohammad Anowar Hossain, Mohammad Muzahidul Islam, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

JOURNAL OF PLANT PHYSIOLOGY 169 ( 10 ) 1012 - 1016 2012年7月

記述言語：英語出版者・発行元：ELSEVIER GMBH, URBAN & FISCHER VERLAG

Methyl jasmonate (MeJA)-induced stomatal closure is accompanied by the accumulation of hydrogen peroxide (H2O2) in guard cells. In this study, we investigated the roles of catalases (CATs) in MeJA-induced stomatal closure using cat mutants cat2, cat3-1 and cat1 cat3, and the CAT inhibitor, 3-aminotriazole (AT). When assessed with 2',7'-dichlorodihydrofluorescein, the reduction of catalase activity by means of mutations and the inhibitor accumulated higher basal levels of H2O2 in guard cells whereas they did not affect stomatal aperture in the absence of MeJA. In contrast, the cat mutations and the treatment with AT potentiated MeJA-induced stomatal closure and MeJA-induced H2O2 production. These results indicate that CATs negatively regulate H2O2 accumulation in guard cells and suggest that inducible H2O2 production rather than constitutive elevation modulates stomatal apertures in Arabidopsis. (C) 2012 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.jplph.2012.03.006

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