研究者詳細 - 中村　宜督

Quercetin Attenuates Acetaldehyde-Induced Cytotoxicity via the Heme Oxygenase-1-Dependent Antioxidant Mechanism in Hepatocytes 招待査読国際誌

Kexin Li, Minori Kidawara, Qiguang Chen, Shintaro Munemasa, Yoshiyuki Murata, Toshiyuki Nakamura, Yoshimasa Nakamura

International Journal of Molecular Sciences 25 ( 16 ) 2024年8月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

It is still unclear whether or how quercetin influences the toxic events induced by acetaldehyde in hepatocytes, though quercetin has been reported to mitigate alcohol-induced mouse liver injury. In this study, we evaluated the modulating effect of quercetin on the cytotoxicity induced by acetaldehyde in mouse hepatoma Hepa1c1c7 cells, the frequently used cellular hepatocyte model. The pretreatment with quercetin significantly inhibited the cytotoxicity induced by acetaldehyde. The treatment with quercetin itself had an ability to enhance the total ALDH activity, as well as the ALDH1A1 and ALDH3A1 gene expressions. The acetaldehyde treatment significantly enhanced the intracellular reactive oxygen species (ROS) level, whereas the quercetin pretreatment dose-dependently inhibited it. Accordingly, the treatment with quercetin itself significantly up-regulated the representative intracellular antioxidant-related gene expressions, including heme oxygenase-1 (HO-1), glutamate-cysteine ligase, catalytic subunit (GCLC), and cystine/glutamate exchanger (xCT), that coincided with the enhancement of the total intracellular glutathione (GSH) level. Tin protoporphyrin IX (SNPP), a typical HO-1 inhibitor, restored the quercetin-induced reduction in the intracellular ROS level, whereas buthionine sulphoximine, a representative GSH biosynthesis inhibitor, did not. SNPP also cancelled the quercetin-induced cytoprotection against acetaldehyde. These results suggest that the low-molecular-weight antioxidants produced by the HO-1 enzymatic reaction are mainly attributable to quercetin-induced cytoprotection.

DOI： 10.3390/ijms25169038

Evaluation of quercetin as a potential cytoprotector against acetaldehyde using the cultured hepatocyte model with aldehyde dehydrogenase isozyme deficiency. 査読国際誌

Yuhang Xu, Takeshi Sawamoto, Ruitong Sun, Aki Ishikura, Shintaro Munemasa, Yoshiyuki Murata, Ayano Satoh, Akiko Matsumoto, Toshiyuki Nakamura, Yoshimasa Nakamura

Bioscience, biotechnology, and biochemistry 2024年7月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Protective effect of quercetin against acetaldehyde was evaluated using the cultured hepatocyte models with aldehyde dehydrogenase (ALDH) isozyme deficiency (aldh2-kd and aldh1a1-kd). The quercetin-induced cytoprotection against acetaldehyde in the ALDH1A1-deficient mutant (aldh1a1-kd) was weaker than that in wild type. Furthermore, quercetin did not enhance the ALDH activity in aldh1a1-kd cells, suggesting that ALDH1A1 is involved in the quercetin-induced cytoprotection.

DOI： 10.1093/bbb/zbae100

Cycloartenyl Ferulate Is the Predominant Compound in Brown Rice Conferring Cytoprotective Potential against Oxidative Stress-Induced Cytotoxicity 招待査読

Hongyan Wu, Toshiyuki Nakamura, Yingnan Guo, Riho Matsumoto, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

International Journal of Molecular Sciences 24 ( 1 ) 822 - 822 2023年1月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Since brown rice extract is a rich source of biologically active compounds, the present study is aimed to quantify the major compounds in brown rice and to compare their cytoprotective potential against oxidative stress. The content of the main hydrophobic compounds in brown rice followed the order of cycloartenyl ferulate (CAF) (89.00 ± 8.07 nmol/g) >> α-tocopherol (αT) (19.73 ± 2.28 nmol/g) > γ-tocotrienol (γT3) (18.24 ± 1.41 nmol/g) > α-tocotrienol (αT3) (16.02 ± 1.29 nmol/g) > γ-tocopherol (γT) (3.81 ± 0.40 nmol/g). However, the percent contribution of CAF to the radical scavenging activity of one gram of whole brown rice was similar to those of αT, αT3, and γT3 because of its weaker antioxidant activity. The CAF pretreatment displayed a significant cytoprotective effect on the hydrogen peroxide-induced cytotoxicity from 10 µM, which is lower than the minimal concentrations of αT and γT required for a significant protection. CAF also enhanced the nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation coincided with the enhancement of the heme oxygenase-1 (HO-1) mRNA level. An HO-1 inhibitor, tin protoporphyrin IX (SnPP), significantly impaired the cytoprotection of CAF. The cytoprotective potential of CAF is attributable to its cycloartenyl moiety besides the ferulyl moiety. These results suggested that CAF is the predominant cytoprotector in brown rice against hydrogen peroxide-induced cytotoxicity.

DOI： 10.3390/ijms24010822

The microbiota catabolites of quercetin glycosides concertedly enhance the resistance against acetaldehyde-induced oxidative stress. 査読国際誌

Kexin Li, Hongyan Wu, Minori Kidawara, Yun Lin, Ayano Satoh, Gongliang Zhang, Shintaro Munemasa, Yoshiyuki Murata, Toshiyuki Nakamura, Yoshimasa Nakamura

Free radical research 56 ( 9-10 ) 1 - 14 2022年12月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

3,4-Dihydroxyphenylacetic acid (DOPAC) and 3-hydroxyphenylacetic acid (OPAC) are the predominant catabolites of quercetin glycosides, such as quercetin 4'-O-β-glucoside from the onion, produced by intestinal microbiota. Although each catabolite has been reported to protect the cells from acetaldehyde-induced cytotoxicity, the effect of their combination remains to be clarified. The purpose of this study was to determine whether the combination of DOPAC and OPAC enhances the resistance against the acetaldehyde-induced oxidative stress in the cultured hepatocytes. The pretreatment of the combination of DOPAC (5 μM) and OPAC (5 μM) showed the significant protection against the acetaldehyde- and hydrogen peroxide-induced cytotoxicity, even though each compound at the same concentration did not. This combination also significantly inhibited the intracellular dichlorofluorescin diacetate-detectable reactive oxygen species (ROS) level, whereas the solo treatment did slightly, suggesting that reducing mechanisms of ROS or compounds that enhance ROS production are involved in the cytoprotective effect. The combinatory treatment significantly enhanced the gene expression of not only the aldehyde dehydrogenases (ALDHs), but also glutamate-cysteine ligase, catalytic subunit, the first rate-limiting enzyme of glutathione (GSH) synthesis. Accordingly, both the intracellular GSH level and the total ALDH activity were enhanced by DOPAC plus OPAC. Involvement of GSH in the cytoprotection as well as ALDH up-regulation by the combination were confirmed by the experiments using a GSH biosynthesis inhibitor, buthionine sulfoximine. Taken together, the present results suggested that the quercetin microbiota catabolites concertedly protect the cells from acetaldehyde through a pre-enhanced resistance against oxidative stress by the GSH-dependent up-regulation of ALDHs.

DOI： 10.1080/10715762.2022.2159820

Benzyl isothiocyanate and its metabolites inhibit cell proliferation through protein modification in mouse preosteoclast RAW264.7 cells. 査読国際誌

Toshiyuki Nakamura, Chiharu Tsutsui, Yu Okuda, Naomi Abe-Kanoh, Saori Okazawa, Shintaro Munemasa, Yoshiyuki Murata, Yoji Kato, Yoshimasa Nakamura

Journal of biochemical and molecular toxicology e23184 2022年8月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Benzyl isothiocyanate (BITC), derived from cruciferous vegetables, is an organosulfur compound exerting antiproliferative effects in several human cancer cells. In this study, we assessed BITC as a potential osteoclastogenesis inhibitor and investigated its underlying mechanism. BITC at 5 μM significantly decreased the viability of the osteoclast-like differentiating RAW264.7 cells, coinciding with the downregulation of the primary biomarkers for osteoclast differentiation, such as the tartrate-resistant acid phosphatase activity and nuclear factor of activated T-cells gene expression. Not only BITC but also its metabolites, inhibited cell proliferation in the normal RAW264.7 cells, suggesting that BITC shows an anti-osteoclastogenesis effect in vivo after its ingestion and metabolism, possibly through an antiproliferative action. Both BITC and its metabolites also enhanced the DNA fragmentation and the caspase-3 activity, whereas their higher concentrations tended to suppress these effects. BITC was intracellularly accumulated when the cells were treated with its metabolites via their degradation into the free form. A quantitative experiment using the proteolysis/high performance liquid chromatography technique showed that the amount of BITC-lysine thiourea in the cells was also increased in a time-dependent manner, suggesting that lysine modification of the cellular proteins actually took place in the cells treated by BITC. Among the cellular proteins, the cleaved caspase-3 was identified as a potential target for lysine modification by BITC. Taken together, BITC dissociated from its metabolites as well as its free form might modulate osteoclastogenesis, possibly through inhibition of cell proliferation by protein modification.

DOI： 10.1002/jbt.23184

A Major Intestinal Catabolite of Quercetin Glycosides, 3-Hydroxyphenylacetic Acid, Protects the Hepatocytes from the Acetaldehyde-Induced Cytotoxicity through the Enhancement of the Total Aldehyde Dehydrogenase Activity 招待査読国際誌

Yujia Liu, Takumi Myojin, Kexin Li, Ayuki Kurita, Masayuki Seto, Ayano Motoyama, Xiaoyang Liu, Ayano SATOH, Shintaro Munemasa, Yoshiyuki Murata, Toshiyuki Nakamura, Yoshimasa Nakamura

International Journal of Molecular Sciences 23 ( 3 ) 2022年2月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Aldehyde dehydrogenases (ALDHs) are the major enzyme superfamily for the aldehyde metabolism. Since the ALDH polymorphism leads to the accumulation of acetaldehyde, we considered that the enhancement of the liver ALDH activity by certain food ingredients could help prevent alcohol-induced chronic diseases. Here, we evaluated the modulating effects of 3-hydroxyphenylacetic acid (OPAC), the major metabolite of quercetin glycosides, on the ALDH activity and acetaldehyde-induced cytotoxicity in the cultured cell models. OPAC significantly enhanced the total ALDH activity not only in mouse hepatoma Hepa1c1c7 cells, but also in human hepatoma HepG2 cells. OPAC significantly increased not only the nuclear level of aryl hydrocarbon receptor (AhR), but also the AhR-dependent reporter gene expression, though not the nuclear factor erythroid-2-related factor 2 (Nrf2)-dependent one. The pretreatment of OPAC at the concentration required for the ALDH upregulation completely inhibited the acetaldehyde-induced cytotoxicity. Silencing AhR impaired the resistant effect of OPAC against acetaldehyde. These results strongly suggested that OPAC protects the cells from the acetaldehyde-induced cytotoxicity, mainly through the AhR-dependent and Nrf2-independent enhancement of the total ALDH activity. Our findings suggest that OPAC has a protective potential in hepatocyte models and could offer a new preventive possibility of quercetin glycosides for targeting alcohol-induced chronic diseases.

DOI： 10.3390/ijms23031762

Characterization of benzyl isothiocyanate extracted from mashed green papaya by distillation. 査読国際誌

Nakamura T, Murata Y, Nakamura Y

Food chemistry 299 125118 - 125118 2019年11月

Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12. 査読国際誌

Naomi Abe-Kanoh, Narumi Kunisue, Takumi Myojin, Ayako Chino, Shintaro Munemasa, Yoshiyuki Murata, Ayano Satoh, Hisao Moriya, Yoshimasa Nakamura

Scientific reports 9 ( 1 ) 8866 - 8866 2019年6月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Benzyl isothiocyanate (BITC) is a naturally-occurring isothiocyanate derived from cruciferous vegetables. BITC has been reported to inhibit the proliferation of various cancer cells, which is believed to be important for the inhibition of tumorigenesis. However, the detailed mechanisms of action remain unclear. In this study, we employed a budding yeast Saccharomyces cerevisiae as a model organism for screening. Twelve genes including MTW1 were identified as the overexpression suppressors for the antiproliferative effect of BITC using the genome-wide multi-copy plasmid collection for S. cerevisiae. Overexpression of the kinetochore protein Mtw1 counteracts the antiproliferative effect of BITC in yeast. The inhibitory effect of BITC on the proliferation of human colon cancer HCT-116 cells was consistently suppressed by the overexpression of Mis12, a human orthologue of Mtw1, and enhanced by the knockdown of Mis12. We also found that BITC increased the phosphorylated and ubiquitinated Mis12 level with consequent reduction of Mis12, suggesting that BITC degrades Mis12 through an ubiquitin-proteasome system. Furthermore, cell cycle analysis showed that the change in the Mis12 level affected the cell cycle distribution and the sensitivity to the BITC-induced apoptosis. These results provide evidence that BITC suppresses cell proliferation through the post-transcriptional regulation of the kinetochore protein Mis12.

DOI： 10.1038/s41598-019-45248-2

Physiological relevance of covalent protein modification by dietary isothiocyanates 査読

Toshiyuki Nakamura, Naomi AbeKanoh, Yoshimasa Nakamura

Journal of Clinical Biochemistry and Nutrition 62 ( 1 ) 11 - 19 2018年1月

Flavonoid metabolism: the interaction of metabolites and gut microbiota 査読

Kaeko Murota, Yoshimasa Nakamura, Mariko Uehara

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 82 ( 4 ) 600 - 610 2018年

Benzyl isothiocyanate ameliorates acetaldehyde-induced cytotoxicity by enhancing aldehyde dehydrogenase activity in murine hepatoma Hepa1c1c7 cells 査読

Yujia Liu, Momoko Yamanaka, Naomi Abe-Kanoh, Xiaoyang Liu, Beiwei Zhu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

FOOD AND CHEMICAL TOXICOLOGY 108 305 - 313 2017年10月

3,4-Dihydroxyphenylacetic acid is a predominant biologically-active catabolite of quercetin glycosides 査読

Yue Tang, Sayaka Nakashima, Shunya Saiki, Yui Myoi, Naomi Abe, Shoko Kuwazuru, Beiwei Zhu, Hitoshi Ashida, Yoshiyuki Murata, Yoshimasa Nakamura

FOOD RESEARCH INTERNATIONAL 89 716 - 723 2016年11月

Nuclear factor-kappaB sensitizes to benzyl isothiocyanate-induced antiproliferation in p53-deficient colorectal cancer cells 査読

N. Abe, D-X Hou, S. Munemasa, Y. Murata, Y. Nakamura

CELL DEATH & DISEASE 5 2014年11月

Electrophiles in Foods: The Current Status of Isothiocyanates and Their Chemical Biology 招待査読

Yoshimasa Nakamura, Noriyuki Miyoshi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 74 ( 2 ) 242 - 255 2010年2月

JNK-dependent NFATc1 pathway positively regulates IL-13 gene expression induced by (-)-epigallocatechin-3-gallate in human basophilic KU812 cells 査読

Haitao Wu, Hang Qi, Dai Iwasaki, Beiwei Zhu, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

FREE RADICAL BIOLOGY AND MEDICINE 47 ( 7 ) 1028 - 1038 2009年10月

Docosahexaenoic acid induces ERK1/2 activation and neuritogenesis via intracellular reactive oxygen species production in human neuroblastoma SH-SY5Y cells 査読

Haitao Wu, Sanae Ichikawa, Chiharu Tani, Beiwei Zhu, Mikiro Tada, Yasuaki Shimoishi, Yoshiyuki Murata, Yoshimasa Nakamura

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 1791 ( 1 ) 8 - 16 2009年1月

ATP depletion alters the mode of cell death induced by benzyl isothiocyanate. 査読

Miyoshi N, Watanabe E, Osawa T, Okuhira M, Murata Y, Ohshima H, Nakamura Y

Biochimica et biophysica acta 1782 ( 10 ) 566 - 573 2008年10月

Reactive oxygen species and angiogenesis: NADPH oxidase as target for cancer therapy 招待査読

Masuko Ushio-Fukai, Yoshimasa Nakamura

CANCER LETTERS 266 ( 1 ) 37 - 52 2008年7月

Role of protein tyrosine phosphatase 1B in vascular endothelial growth factor signaling and cell-cell adhesions in endothelial cells 査読

Yoshimasa Nakamura, Nikolay Patrushev, Hyoe Inomata, Dolly Mehta, Norifumi Urao, Ha Won Kim, Masooma Razvi, Vidisha Kini, Kalyankar Mahadev, Barry J. Goldstein, Ronald McKinney, Tohru Fukai, Masuko Ushio-Fukai

CIRCULATION RESEARCH 102 ( 10 ) 1182 - 1191 2008年5月

Papaya seed represents a rich source of biologically active isothiocyanate 査読

Yoshimasa Nakamura, Motoko Yoshimoto, Yoshiyuki Murata, Yasuaki Shimoishi, Yumi Asai, Eun Young Park, Kenji Sato, Yasushi Nakamura

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 55 ( 11 ) 4407 - 4413 2007年5月

Selective cytotoxicity of benzyl isothiocyanate in the proliferating fibroblastoid cells 査読

Noriyuki Miyoshi, Koji Uchida, Toshihiko Osawa, Yoshimasa Nakamura

INTERNATIONAL JOURNAL OF CANCER 120 ( 3 ) 484 - 492 2007年2月

Benzyl isothiocyanate inhibits excessive superoxide generation in inflammatory leukocytes: implication for prevention against inflammation-related carcinogenesis 査読

N Miyoshi, S Takabayashi, T Osawa, Y Nakamura

CARCINOGENESIS 25 ( 4 ) 567 - 575 2004年4月

A link between benzyl isothiocyanate-induced cell cycle arrest and apoptosis: Involvement of mitogen-activated protein kinases in the Bcl-2 phosphorylation 査読

N Miyoshi, K Uchida, T Osawa, Y Nakamura

CANCER RESEARCH 64 ( 6 ) 2134 - 2142 2004年3月

Arachidonic acid cascade inhibitors modulate phorbol ester-induced oxidative stress in female ICR mouse skin: Differential roles of 5-lipoxygenase and cyclooxygenase-2 in leukocyte infiltration and activation 査読

Y Nakamura, M Kozuka, K Naniwa, S Takabayashi, K Torikai, R Hayashi, T Sato, H Ohigashi, T Osawa

FREE RADICAL BIOLOGY AND MEDICINE 35 ( 9 ) 997 - 1007 2003年11月

Pivotal role of electrophilicity in glutathione S-transferase induction by tert-butylhydroquinone 査読

Y Nakamura, T Kumagai, C Yoshida, Y Naito, M Miyamoto, H Ohigashi, T Osawa, K Uchida

BIOCHEMISTRY 42 ( 14 ) 4300 - 4309 2003年4月

Involvement of the mitochondrial death pathway in chemopreventive benzyl isothiocyanate-induced apoptosis 査読

Y Nakamura, M Kawakami, A Yoshihiro, N Miyoshi, H Ohigashi, K Kawai, T Osawa, K Uchida

JOURNAL OF BIOLOGICAL CHEMISTRY 277 ( 10 ) 8492 - 8499 2002年3月

Ebselen, a glutathione peroxidase mimetic seleno-organic compound, as a multifunctional antioxidant - Implication for inflammation-associated carcinogenesis 査読

Y Nakamura, Q Feng, T Kumagai, K Torikai, H Ohigashi, T Osawa, N Noguchi, E Niki, K Uchida

JOURNAL OF BIOLOGICAL CHEMISTRY 277 ( 4 ) 2687 - 2694 2002年1月

A catechol antioxidant protocatechuic acid potentiates inflammatory leukocyte-derived oxidative stress in mouse skin via a tyrosinase bioactivation pathway 査読

Y Nakamura, K Torikai, H Ohigashi

FREE RADICAL BIOLOGY AND MEDICINE 30 ( 9 ) 967 - 978 2001年5月

A simple phenolic antioxidant protocatechuic acid enhances tumor promotion and oxidative stress in female ICR mouse skin: dose- and timing-dependent enhancement and involvement of bioactivation by tyrosinase 査読

Y Nakamura, K Torikai, Y Ohto, A Murakami, T Tanaka, H Ohigashi

CARCINOGENESIS 21 ( 10 ) 1899 - 1907 2000年10月

A glutathione S-transferase inducer from papaya, rapid screening, identification and structure-activity relationship of isothiocyanates 査読

Y Nakamura, Y Morimitsu, T Uzu, H Ohigashi, A Murakami, Y Naito, Y Nakagawa, T Osawa, K Uchida

CANCER LETTERS 157 ( 2 ) 193 - 200 2000年9月

Redox regulation of glutathione S-transferase induction by benzyl isothiocyanate: Correlation of enzyme induction with the formation of reactive oxygen intermediates 査読

Y Nakamura, H Ohigashi, S Masuda, A Murakami, Y Morimitsu, Y Kawamoto, T Osawa, M Imagawa, K Uchida

CANCER RESEARCH 60 ( 2 ) 219 - 225 2000年1月

Suppression of tumor promoter-induced oxidative stress and inflammatory responses in mouse skin by a superoxide generation inhibitor 1 '-acetoxychavicol acetate 査読

Y Nakamura, A Murakami, Y Ohto, K Torikai, T Tanaka, H Ohigashi

CANCER RESEARCH 58 ( 21 ) 4832 - 4839 1998年11月

Inhibitory effects of curcumin and tetrahydrocurcuminoids on the tumor promoter-induced reactive oxygen species generation in leukocytes in vitro and in vivo 査読

Yoshimasa Nakamura, Yoshimi Ohto, Akira Murakami, Toshihiko Osawa, Hajime Ohigashi

Japanese Journal of Cancer Research 89 ( 4 ) 361 - 370 1998年

Inhibitory effect of pheophorbide α, a chlorophyll-related compound, on skin tumor promotion in ICR mouse 査読

Yoshimasa Nakamura, Akira Murakami, Koichi Koshimizu, Hajime Ohigashi

Cancer Letters 108 ( 2 ) 247 - 255 1996年11月

Royal jelly exosomes as a stable and bioavailable carrier for fucoxanthin: A novel approach for antimelanoma therapy 査読

Yujing Lu, Jiarui Jin, Yuchen Yang, Chenglei Qiu, Yoshimasa Nakamura, Hang Qi

Food Bioscience 2025年9月

Involvement of reactive carbonyl species in inhibition of germination and seedling growth by salt stress in rice 招待

Faruk Hossain Khan, Yoshitaka Nakashima, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshihiko Hirai, Md Anamul Hoque, Mohammad Saidur Rhaman, Shintaro Munemasa, Jun'ichi Mano, Yoshiyuki Murata

Bioscience, Biotechnology, and Biochemistry 2025年7月

Proteome analysis reveals the molecular mechanisms of fucoxanthin-induced apoptosis in glial cells PC12 after nanoencapsulation 査読

Chunyan Wang, Xinxin Ma, Yoshimasa Nakamura, Hang Qi

Journal of Functional Foods 126 2025年3月

Fraglide-1 from traditional Chinese aromatic vinegar: A natural AhR antagonist for atopic dermatitis. 査読国際誌

Kosuke Kato, Miki Akamatsu, Saya Kakimaru, Mayuko Koreishi, Masahiro Takagi, Masahiro Miyashita, Yoshiyuki Murata, Yoshimasa Nakamura, Ayano Satoh, Yoshio Tsujino

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 115301 - 115301 2025年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Traditional Chinese Zhenjiang aromatic vinegar (Kozu) contains Fraglide-1 (FG1), a bioactive lactone with demonstrated peroxisome proliferator-activated receptor gamma (PPARγ) agonist and antioxidant activities. This study explored FG1's novel ability to antagonize the aryl hydrocarbon receptor (AhR) signaling pathway, which regulates artemin expression and contributes to itching and inflammation in atopic dermatitis. Through molecular docking simulations and cell-based assays in human keratinocytes, we demonstrated FG1's potent antagonistic activity against AhR signaling. FG1 effectively suppressed FICZ-induced inflammatory responses, including artemin expression, with potency (half maximal inhibitory concentration, IC50 = 5.1 μM) comparable to the synthetic antagonist StemRegenin 1 (SR1) while demonstrating a superior safety profile (median lethal concentration, LC50 > 100 μM vs. 27.5 μM for SR1). These findings expand our understanding of bioactive compounds from traditional fermented foods and their regulatory effects on AhR signaling, providing a foundation for future studies on FG1's role in modulating skin inflammation.

DOI： 10.1016/j.fct.2025.115301

Dual role of cytosolic GSH in the ABA signaling pathway and plasma membrane ion channel regulation in guard cells of Vicia faba. 査読国際誌

Huifei Yin, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

Journal of plant physiology 306 154447 - 154447 2025年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Abscisic acid (ABA) induces stomatal closure in higher plants under drought stress. Glutathione (GSH) negatively regulates ABA-induced stomatal closure and reactive carbonyl species (RCS) play a role as signal mediators downstream of reactive oxygen species production in ABA signaling pathway in Arabidopsis thaliana. Activation of slow (S-type) anion channels and inhibition of inward-rectifying potassium ion (Kin+) channels in the plasma membrane are essential for ABA-induced stomatal closure. However, there is limited evidence regarding role of GSH in the activation of S-type anion channels and the inhibition of Kin+ channels. We used Vicia faba to clarify the regulation of these ion channels by GSH and RCS. Pretreatment of guard-cell protoplasts with the GSH-supplementing agent, glutathione monoethyl ester (GSHmee), suppressed the activation of S-type anion channels and the inactivation of Kin+ channels induced by ABA. The pretreatment with the RCS scavenger carnosine suppressed the activation of S-type anion channels and the inactivation of Kin+ channels by ABA. On patch clamping guard-cell protoplasts, the addition of GSH to the pipette (cytosolic) buffer decreased the S-type anion currents and increased the Kin+ currents. These results suggest that cytosolic GSH is involved in ABA-induced stomatal closure via negative regulation of ABA signaling and via direct regulation of ion channel activities in V. faba.

DOI： 10.1016/j.jplph.2025.154447

TGG1 and TGG2 mutations impair allyl isothiocyanate-mediated stomatal closure in Arabidopsis thaliana. 査読国際誌

Kadri Oumaima, Mohammad Shakhawat Hossain, Wenxiu Ye, Eiji Okuma, Mohammad Issak, Mohammad Mahbub Islam, Misugi Uraji, Yoshimasa Nakamura, Izumi C Mori, Shintaro Munemasa, Yoshiyuki Murata

Protoplasma 2025年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Myrosinase, referred to as thioglucoside glucohydrolase (TGG), plays a crucial role in plant physiology through catalyzing the hydrolysis of glucosinolates into bioactive isothiocyanates. In Arabidopsis thaliana, the myrosinases TGG1 and TGG2 are essential for abscisic acid- and methyl jasmonate-induced stomata closure. Allyl isothiocyanate (AITC), one of myrosinase products, triggers stomatal closure in A. thaliana. We investigated stomatal responses to AITC to clarify the role of TGG1 and TGG2 in Arabidopsis guard-cell signaling. Allyl isothiocyanate at 50 μM and 100 μM induced stomatal closure in the tgg1 and tgg2 single mutants but not in the tgg1 tgg2 double mutant. Furthermore, AITC at 50 μM induced the production of reactive oxygen species and nitric oxide, cytosolic alkalization, and oscillations in cytosolic free calcium concentration in guard cells of both wild-type and mutant plants. These findings suggest that TGG1 and TGG2 are involved in AITC signaling pathway through interaction with signal component(s) downstream of these signaling events, which is not accompanied by hydrolysis of glucosinolates because of the difference in subcellular localization between enzymes (myrosinases) and substrates (glucosinolates).

DOI： 10.1007/s00709-025-02039-z

Resveratrol, a food-derived polyphenol, promotes Melanosomal degradation in skin fibroblasts through coordinated activation of autophagy, lysosomal, and antioxidant pathways 査読

Saki Okamoto, Saya Kakimaru, Mayuko Koreishi, Mika Sakamoto, Yoshimasa Nakamura, Hideya Ando, Yoshio Tsujino, Ayano Satoh

Journal of Functional Foods 125 2025年2月

Reactive carbonyl species function downstream of reactive oxygen species in chitosan-induced stomatal closure. 査読国際誌

Israt Jahan, Md Moshiul Islam, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Jun'ichi Mano, Yoshiyuki Murata

Physiologia plantarum 177 ( 1 ) e70094 2025年

記述言語：英語掲載種別：研究論文（学術雑誌）

An elicitor, chitosan (CHT), induces stomatal closure in plants, which is accompanied by salicylhydroxamic acid (SHAM)-sensitive peroxidases-mediated reactive oxygen species (ROS) production in guard cells. Reactive carbonyl species (RCS) function downstream of ROS in abscisic acid (ABA) and methyl jasmonate (MeJA) signalling in guard cells. However, the involvement of RCS in CHT-induced stomatal closure is still unknown. In this study, we used transgenic tobacco (Nicotiana tabacum) plants overexpressing Arabidopsis thaliana 2-alkenal reductase (AER-OE tobacco) and Arabidopsis wild-type (WT) plants to investigate whether RCS is involved in CHT-induced stomatal closure. Chitosan-induced stomatal closure was inhibited in the tobacco AER-OE plants. In the WT tobacco and Arabidopsis plants, CHT-induced stomatal closure was inhibited by RCS scavengers, carnosine and pyridoxamine. Chitosan significantly increased RCS production in the WT tobacco and Arabidopsis, but in the tobacco AER-OE plants, chitosan did not increase significantly RCS accumulation. Moreover, neither the application of RCS scavengers to both WT plants nor scavenging RCS by AER-OE affected the CHT-induced ROS accumulation. However, treatment with a peroxidase inhibitor, SHAM, significantly inhibited CHT-induced RCS accumulation in WT tobacco and Arabidopsis plants. Taken together, these results suggest that RCS acts downstream of ROS production in CHT signalling in guard cells of A. thaliana and N. tabacum.

DOI： 10.1111/ppl.70094

CNGC2 Negatively Regulates Stomatal Closure and Is Not Required for flg22- and H2O2-Induced Guard Cell [Ca2+]cyt Elevation in Arabidopsis thaliana. 査読国際誌

Rojina Akter, Yasuhiro Inoue, Saori Masumoto, Yoshiharu Mimata, Takakazu Matsuura, Izumi C Mori, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata, Shintaro Munemasa

Physiologia plantarum 177 ( 4 ) e70396 2025年

記述言語：英語掲載種別：研究論文（学術雑誌）

In guard cells, cytosolic Ca2+ acts as a second messenger that mediates abscisic acid (ABA)- and pathogen-associated molecular pattern (PAMP)-induced stomatal closure. It was reported that Arabidopsis cyclic nucleotide-gated ion channel 2 (CNGC2) functions as hydrogen peroxide (H2O2)- and PAMP-activated Ca2+-permeable channels at the plasma membrane of mesophyll cells and mediates Ca2+-dependent PAMP-triggered immunity. In this study, we examined the role of CNGC2 in the regulation of stomatal movement because CNGC2 is also expressed in guard cells. We found that stomata of the CNGC2 disruption mutant cngc2-3 are constitutively closed even in the absence of ABA or the flagellar-derived PAMP, flg22. Consistently, leaf temperatures of the cngc2-3 mutant were higher than those of wild-type (WT) plants. The stomatal phenotype of the cngc2-3 mutant was restored by complementation with wild-type CNGC2 under the control of the guard cell preferential promoter, pGC1. Elevation of cytosolic free Ca2+ concentration in guard cells induced by flg22 and H2O2 remained intact in the cngc2-3 mutant. The introduction of the ost1-3 mutation into the cngc2-3 background did not alter the stomatal phenotype. However, the stomatal phenotype of the cngc2-3 mutant was successfully rescued in the double disruption mutant cngc2-3aba2-2. Taken together, these results suggest that CNGC2 negatively regulates stomatal closure response and does not function as flg22- and H2O2-activated Ca2+ channels in guard cells. Though CNGC2 is responsive for H2O2- and flg22-induced [Ca2+]cyt elevation in mesophyll cells, the involvement of CNGC2 in the response to H2O2 and flg22 in guard cells is questionable.

DOI： 10.1111/ppl.70396

Polyphenol oxidase mediated (-)-epigallocatechin gallate stabilized protein in body wall of Apostichopus japonicus: Characteristics and structure. 査読国際誌

Yicheng Guo, Yu Ming, Xiufang Dong, Yoshimasa Nakamura, Xiuping Dong, Hang Qi

Food chemistry 470 142710 - 142710 2024年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Heat treatment is the most common processing method in Apostichopus japonicus (A. japonicus) processing. However, improper heat treatment can lead to the degradation of protein. In an effort to mitigate this occurrence, we looked into how the protein in the body wall of A. japonicus (AJBWP) was affected by polyphenol oxidase (PPO)-mediated (-)-epigallocatechin gallate (EGCG). The results showed that polyphenol oxidase-mediated EGCG induced protein aggregation. Meanwhile, the antioxidant and thermal stability of the protein were enhanced, and the digestive properties of the protein were inhibited. Protein structure studies have shown that polyphenol oxidase mediated EGCG changes the secondary and tertiary structure of proteins and can maintain the microstructure of proteins after heat treatment. In conclusion, polyphenol oxidase-mediated EGCG was a way to improve the thermal stability of body wall proteins and alleviate the thermal degradation of proteins in A. japonicus.

DOI： 10.1016/j.foodchem.2024.142710

Advanced characterization of fish skin gelatin-proanthocyanidins covalent and non-covalent composite emulsions for benzyl isothiocyanate delivery. 査読国際誌

Yuhang Shen, Songyi Lin, Liang Song, Peng Sun, Yoshimasa Nakamura, Yue Tang

International journal of biological macromolecules 139071 - 139071 2024年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

This research endeavored to engineer robust delivery matrices for bioactives, specifically benzyl isothiocyanate (BITC), by harnessing the synergistic covalent and non-covalent interactions between fish skin gelatin (FSG) and proanthocyanidins (PC) to synthesize novel composite emulsions. The objective was to delineate the influence of these molecular interactions on the emulsion's structural integrity and stability, which are pivotal for the efficacious encapsulation and controlled release of BITC. Employing a suite of analytical techniques, including Fourier transform infrared spectroscopy (FTIR), fluorescence spectroscopy, and contact angle measurements, the study delineated the predominant molecular forces at play within the FSG-PC complex, identifying electrostatic and hydrophobic interactions as the cornerstones of this interaction. An assessment of the emulsions' physicochemical properties, encompassing chromaticity, antioxidant efficacy, microstructural attributes, particle dimensions, zeta potential, and BITC retention, was undertaken to discern the optimal encapsulation strategy. The data unequivocally indicated that emulsions enriched with 0.06 wt% PC, in non-covalent synergy with FSG, afforded the most pronounced stability and retention of BITC. This work paves the way for future studies and the translational application of FSG-PC composite emulsions in the realm of bioactive substance delivery, offering a promising avenue for innovation in pharmaceutical and nutraceutical formulations.

DOI： 10.1016/j.ijbiomac.2024.139071

GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 functions upstream of reactive carbonyl species production in Arabidopsis guard-cell abscisic acid signaling. 査読国際誌

Oumayma Shaiek, Huifei Yin, Nodoka Uesako, Md Moshiul Islam, Mohammad Saidur Rhaman, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Jun'ichi Mano, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 2024年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1), a leucine-rich repeat receptor-like kinase, is involved in abscisic acid (ABA)-induced stomatal closure. We investigated the role of GHR1 in reactive oxygen species (ROS) signaling for ABA-induced stomatal closure. Abscisic acid induced ROS production in wild type (WT) and the ghr1 of Arabidopsis thaliana. Hydrogen peroxide induced stomatal closure, accompanying the generation of acrolein in guard cells. The reactive carbonyl species (RCS) scavengers inhibited the ABA- and H2O2-induced stomatal closure in WT. In the ghr1, H2O2 failed to induce acrolein production and stomatal closure while RCS induced stomatal closure. Thus, GHR1 functions downstream of ROS and is required for the generation of RCS in guard-cell ABA signaling. In the ghr1, Ca2+ induced stomatal closure but RCS did not activate ICa channels. The GHR1 may be also involved in a Ca2+-independent pathway for ABA-induced stomatal closure.

DOI： 10.1093/bbb/zbae135

Mechanism of inhibition of melanoma by fucoxanthin simulated in vitro digestion products in cell models constructed using human malignant melanoma cells (A375) and keratinocytes (HaCaT). 査読国際誌

Yujing Lu, Yida Wang, Tian Yao, Xiufang Dong, Yu Liu, Yoshimasa Nakamura, Hang Qi

Food chemistry 462 141003 - 141003 2024年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Recently, the increasing incidence of malignant melanoma has become a major public health concern owing to its poor prognosis and impact on quality of life. Consuming foods with potent antitumor compounds can help prevent melanoma and maintain skin health. Fucoxanthin (FX), a naturally occurring carotenoid found in brown algae, possesses antitumor properties. However, its bioavailability, safety risks, and in vivo effects and mechanisms against melanoma remain unclear. This research focused on evaluating the safety and prospective antimelanoma impact of simulated gastrointestinal digestion products (FX-ID) on HaCaT and A375 cells.The results indicate that FX-ID exerts negative effects on mitochondria in A375 cells, increases Bax expression, releases Cytochrome C, and activates cleaved caspase-3, ultimately promoting apoptosis. Additionally, FX-ID influences the mitogen-activated protein kinase (MAPK) pathway by enhancing cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-κB) levels, consequently facilitating apoptosis and inflammation without significantly impacting HaCaT cells. These findings provide insight into inhibitory mechanism of FX-ID against melanoma, guiding the development of functional foods for prevention.

DOI： 10.1016/j.foodchem.2024.141003

Whey protein and flaxseed gum co-encapsulated fucoxanthin promoted tumor cells apoptosis based on MAPK-PI3K/Akt regulation on Huh-7 cell xenografted nude mice. 査読国際誌

Chunyan Wang, Xu Huang, Kailing Sun, Xiang Li, Dingding Feng, Yoshimasa Nakamura, Hang Qi

International journal of biological macromolecules 134838 - 134838 2024年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Fucoxanthin (FX), a non-provitamin-A carotenoid, is a well-known major xanthophyll contained in edible brown algae. The nanoencapsulation of FX was motivated due to its multiple activities. Here, nano-encapsulated-FX (nano-FX) was prepared according to our early method by using whey protein and flaxseed gum as the biomacromolecule carrier material, then in vivo antitumor effect and mechanism of nano-FX on xenograft mice were investigated. Thirty 4-week-old male BALB/c nude mice were fed adaptively for 7 days to establish xenograft tumor model with Huh-7 cells. The tumor-bearing mice consumed nano-FX (50, 25, and 12.5 mg kg-1) and doxorubicin hydrochloride (DOX, 1 mg kg-1) or did not consume (Control) for 21 days, n = 6. The tumor inhibition rates of nano-FX were as high as 54.67 ± 1.04 %. Nano-FX intervention promoted apoptosis and induced hyperchromatic pyknosis and focal necrosis in tumor tissue by down-regulating the expression of p-JNK, p-ERK, PI3Kp85α, p-AKT, p-p38MAPK, Bcl-2, CyclinD1 and Ki-67, while up-regulating the expression of cleaved caspase-3 and Bax. Nano-FX inhibited tumor growth and protected liver function of tumor bearing mice in a dose-dependent manner, up-regulate the level of apoptosis-related proteins, inhibit the MAPK-PI3K/Akt pathways, and promote tumor cell apoptosis.

DOI： 10.1016/j.ijbiomac.2024.134838

The Metabolite of γ-Tocopherol, 2,7,8-Trimethyl-2-(2′-Carboxyethyl)-6-Hydroxychroman, Exerts Intracellular Antioxidant Activity via Up-Regulation of Heme Oxygenase-1 in Hepatocytes 招待査読

Shosuke Aoyama, Tomoka Nishio, Daiki Moriya, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura, Toshiyuki Nakamura

Nutraceuticals 2024年8月

Outward-rectifying potassium channels GORK and SKOR function in regulation of root growth under salt stress in Arabidopsis thaliana. 査読国際誌

Hafsa Jahan Hiya, Yoshitaka Nakashima, Airi Takeuchi, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata, Shintaro Munemasa

Journal of plant physiology 302 154322 - 154322 2024年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Plants often face high salinity as a significant environmental challenge with roots being the first responders to this stress. Maintaining K+/Na+ ratio within plant cells is crucial for survival, as the intracellular K+ level decreases and the intracellular Na+ level increases under saline conditions. However, knowledge about the molecular regulatory mechanisms of K+ loss in response to salt stress through outward-rectifying K+ channels in plants is largely unknown. In this study, we found that the Arabidopsis double mutant gorkskor, in which the GORK and SKOR genes are disrupted, showed an improved primary root growth under salt stress compared to wild-type (WT) and the gork and skor single-mutant plants. No significant differences in the sensitivity to mannitol stress between the WT and gorkskor mutant were observed. Accumulation of ROS induced by salt stress was reduced in the gorkskor roots. The gorkskor mutant seedlings had significantly higher K+ content, lower Na+ content, and a greater resultant K+/Na+ ratio than the WT under salt stress. Moreover, salt-stress-induced elevation of cytosolic free Ca2+ concentration was reduced in the gorkskor roots. Taken together, these results suggest that Arabidopsis Shaker-type outward-rectifying K+ channels GORK and SKOR may redundantly function in regulation of primary root growth under salt stress and are involved in not only the late-stage response (e.g. K+ leakage) but also the early response including ROS production and [Ca2+]cyt elevation.

DOI： 10.1016/j.jplph.2024.154322

Negative regulation of chitosan-induced stomatal closure by glutathione in Arabidopsis thaliana. 査読国際誌

Israt Jahan, Shintaro Munemasa, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 2024年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Chitosan (CHT) is a deacylated derivative of chitin and improves growth and yield performance, activates defensive genes, and also induces stomatal closure in plants. Glutathione (GSH) has significant functions in the growth, development, defense systems, signaling, and gene expression. Glutathione negatively regulates abscisic acid (ABA)-, methyl jasmonate (MeJA)-, and salicylic acid (SA)-induced stomatal closure. However, the negative regulation by GSH of CHT-induced stomatal closure is still unknown. Regulation of CHT-induced stomatal closure by GSH in guard cells was investigated using two GSH-deficient mutants, cad2-1 and ch1-1, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). The cad2-1 and ch1-1 mutations and CDNB treatment enhanced CHT-induced stomatal closure. Treatment with glutathione monoethyl ester (GSHmee) restored the GSH level in the guard cells of cad2-1 and ch1-1 and complemented the stomatal phenotype of the mutants. These results indicate that GSH negatively regulates CHT-induced stomatal closure in A. thaliana.

DOI： 10.1093/bbb/zbae065

Unlocking stability and efficiency in benzyl isothiocyanate-loaded emulsions: Unveiling synergistic interactions of fish skin gelatin and pectin 査読

Liu Wenwen, Lin Songyi, Song Liang, Wang Siyu, Nakamura Yoshimasa, Tang Yue

Food Bioscience 58 103788 - 103788 2024年4月

Polyphenol oxidase mediates (-)-epigallocatechin gallate to inhibit endogenous cathepsin activity in Apostichopus japonicus. 査読国際誌

Yicheng Guo, Yu Ming, Kailing Sun, Xiufang Dong, Yoshimasa Nakamura, Xiuping Dong, Hang Qi

Food chemistry 449 139166 - 139166 2024年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

Apostichopus japonicus (A. japonicus) has rich nutritional value and is an important economic crop. Due to its rich endogenous enzyme system, fresh A. japonicus is prone to autolysis during market circulation and storage, resulting in economic losses. In order to alleviate this phenomenon, we investigated the effect of polyphenol oxidase (PPO) mediated (-)-epigallocatechin gallate (EGCG) on the activity and structure of endogenous cathepsin series protein (CEP) from A. japonicus. Research on cathepsin activity showed that PPO mediated EGCG could significantly reduce enzyme activity, resulting in a decrease in enzymatic reaction rate. SDS-PAGE and scanning electron microscopy results showed that PPO mediates EGCG could induce CEP aggregation to form protein aggregates. Various spectral results indicated that EGCG caused changes in the structure of CEP. Meanwhile, the conjugates formed by PPO mediated EGCG had lower thermal stability. In conclusion, PPO mediated EGCG was an effective method to inhibit the endogenous enzyme activity.

DOI： 10.1016/j.foodchem.2024.139166

Enhancing effect of the coexisting alpha-tocopherol on quercetin absorption and metabolism. 査読国際誌

Rikito Mitsuzane, Reiko Okubo, Miyu Nishikawa, Shinichi Ikushiro, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura, Toshiyuki Nakamura

Free radical research 58 ( 2 ) 1 - 16 2024年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

The aim of this study is to investigate the modulating effect of coexisting food components on the absorption and metabolism of quercetin and blood plasma antioxidant potentials. The combination of quercetin with α-tocopherol (αT), cellulose, or a commercially-available vegetable beverage containing αT and dietary fiber was orally administered to mice. Compared to the single administration of quercetin aglycone, the co-administration of αT with quercetin significantly increased the plasma quercetin concentration at 0.5 h, whereas the combination of quercetin and cellulose decreased it. Interestingly, the administration of quercetin mixed with the vegetable beverage showed no significant change of the quercetin concentration in the mice plasma. The treatment of the cells with the blood plasma after the co-administration of αT with quercetin significantly upregulated the gene expression of the antioxidant enzyme (heme oxygenase-1), whereas the quercetin and cellulose combination did not. In the plasma of the quercetin-administered mice, eight types of quercetin metabolites were detected and their quantities were affected by the combination with αT. The potentials of the heme oxygenase-1 gene expression by these metabolites were very limited, although several metabolites showed radical scavenging activities comparable to aglycone in the in vitro assays. These results suggested that the combination of αT potentiates the quercetin absorption and metabolism and thus the plasma antioxidant potentials, at least in part, by the quantitative changes in the quercetin metabolites.

DOI： 10.1080/10715762.2024.2317206

Aromatic oil from lavender as an atopic dermatitis suppressant 査読国際誌

Haruna Sato, Kosuke Kato, Mayuko Koreishi, Yoshimasa Nakamura, Yoshio Tsujino, Ayano Satoh

PLOS ONE 19 ( 1 ) e0296408 2024年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

In atopic dermatitis (AD), nerves are abnormally stretched near the surface of the skin, making it sensitive to itching. Expression of neurotrophic factor Artemin (ARTN) involved in such nerve stretching is induced by the xenobiotic response (XRE) to air pollutants and UV radiation products. Therefore, AD can be monitored by the XRE response. Previously, we established a human keratinocyte cell line stably expressing a NanoLuc reporter gene downstream of XRE. We found that 6-formylindolo[3,2-b]carbazole (FICZ), a tryptophan metabolite and known inducer of the XRE, increased reporter and Artemin mRNA expression, indicating that FICZ-treated cells could be a model for AD. Lavender essential oil has been used in folk medicine to treat AD, but the scientific basis for its use is unclear. In the present study, we investigated the efficacy of lavender essential oil and its major components, linalyl acetate and linalool, to suppress AD and sensitize skin using the established AD model cell line, and keratinocyte and dendritic cell activation assays. Our results indicated that lavender essential oil from L. angustifolia and linalyl acetate exerted a strong AD inhibitory effect and almost no skin sensitization. Our model is useful in that it can circumvent the practice of using animal studies to evaluate AD medicines.

DOI： 10.1371/journal.pone.0296408

Detection of Simulated In Vitro Digestion Products of Fucoxanthin and Their Photodamage Alleviation Effect in Retinal Müller Cells Induced by Ultraviolet B Irradiation: A Proteomics Analysis. 査読国際誌

Yixin Shi, Yingzhen Wang, Haotian Zou, Baomin Zhao, Taihai Zhu, Yoshimasa Nakamura, Hang Qi

Journal of agricultural and food chemistry 71 ( 40 ) 14626 - 14637 2023年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Our previous study reported that the marine dietary bioactive compound fucoxanthin (FX) has the potential to reduce the level of oxidation in retinal Müller cells (RMCs) induced by ultraviolet B (UVB) irradiation. However, the gastrointestinal environment can inhibit the bioavailability and absorption of FX in the cell systems. In the current study, FX was initially digested in a simulated in vitro gastrointestinal fluid. Nine main digestive products were identified, and the photoprotective activities of FX simulated in vitro gastrointestinal digestion products (FX-ID) were assessed in the same RMC model. FX-ID significantly reduced intracellular ROS and alleviated apoptosis. Western blot assays showed that FX-ID inhibited phosphorylated proteins in the MAPK and NF-κB signaling pathways. Our proteomics analysis revealed that the differentially expressed proteins were linked to biological networks associated with antioxidation and metabolic processes. The data may provide insight into the photoprotective mechanisms of FX-ID and promote the development of various functional foods to prevent retinal disorders.

DOI： 10.1021/acs.jafc.3c03853

The effect of exogenous dihydroxyacetone and methylglyoxal on growth, anthocyanin accumulation, and the glyoxalase system in Arabidopsis. 査読国際誌

Maoxiang Zhao, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Izumi C Mori, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 87 ( 11 ) 1323 - 1331 2023年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Dihydroxyacetone (DHA) occurs in wide-ranging organisms including plants and can undergo spontaneous conversion to methylglyoxal (MG). While the toxicity of MG to plants is well-known, the toxicity of DHA to plants remains to be elucidated. We investigated the effects of DHA and MG on Arabidopsis. Exogenous DHA at up to 10 m m did not affect the radicle emergence, the expansion of green cotyledons, the seedling growth, or the activity of glyoxalase II while DHA at 10 m m inhibited the root elongation and increased the activity of glyoxalase I. Exogenous MG at 1.0 m m inhibited these physiological responses and increased both activities. DHA at 10 m m increased the MG content in the roots. These results indicate that DHA is not so toxic as MG in Arabidopsis seeds and seedlings and suggest that the toxic effect of DHA at high concentrations is attributed to MG accumulation by the conversion to MG.

DOI： 10.1093/bbb/zbad109

Characterisation of zinc‐chelating peptides derived from scallop (Patinopecten yessoensis) mantle and their intestinal transport in everted rat sacs 査読

Xiaoyang Liu, Jiatong Sun, Yue Li, Xuening Yu, Fawen Yin, Deyang Li, Pengfei Jiang, Yoshimasa Nakamura, Dayong Zhou

International Journal of Food Science & Technology 2023年5月

ALDEHYDE DEHYDROGENASES AS POTENTIAL TARGETS FOR ETHANOL-RELATED DISEASES

XU Yuhang, NAKAMURA Toshiyuki, NAKAMURA Yoshimasa

Journal of Environmental Science for Sustainable Society 12 ( Supplement ) MR03_p9 - MR03_p12 2023年4月

担当区分：最終著者,　責任著者記述言語：英語出版者・発行元：Graduate School of Environmental and Life Science, Okayama University

Since acetaldehyde is a toxic metabolite of ethanol, its accumulation causes several acute and chronic diseases related to the excessive intake of alcoholic beverages. Aldehyde dehydrogenases (ALDHs) play an important role in the metabolism of not only exogenous aldehydes, but also endogenous ones including acetaldehyde. Among the ALDH family, ALDH2 mainly contributes to acetaldehyde elimination. This paper reviews the function of ALDH2 as well as auxiliary ALDHs in the ethanol metabolism. This paper also summarizes the regulation of the total ALDH activity by certain food chemicals, providing implication for their therapeutic potential against alcohol-related diseases.

DOI： 10.3107/jesss.12.mr03

Characterization of oyster water-soluble protein-EGCG conjugate and its antioxidant effects on linolic acid in emulsion system 査読

Yue Li, Xiaoyang Liu, Fawen Yin, Deyang Li, Pengfei Jiang, Liang Song, Yoshimasa Nakamura, Dayong Zhou

Food Bioscience 51 102215 - 102215 2023年2月

Polyphenol extracts from Ascophyllum nodosum protected sea cucumber (Apostichopus japonicas) body wall against thermal degradation during tenderization 査読国際誌

Yu Ming, Yingzhen Wang, Yuqianqian Xie, Xiufang Dong, Yoshimasa Nakamura, Xing Chen, Hang Qi

Food Research International 164 112419 - 112419 2023年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier BV

To retard the protein degradation during sea cucumber processing, polyphenol extracts from Ascophyllum nodosum (PhE) was used as a potential antioxidant to maintain the structural integrity of sea cucumber body wall. Accordingly, the protection effects of PhE (0, 0.5, 1.0 and 1.5 mg PhE/g SFBW) against thermal degradation of the solid fragments of body wall (SFBW) have been investigated in order to evaluate their impact on the oxidation level and structural changes. Electronic Spin Resonance results showed that PhE could significantly inhibit the occurrence of oxidation by scavenging the free radicals. The effect of PhE on chemical analysis of soluble matters in SFBW was characterized by SDS-PAGE and HPLC. Compared with thermally treated SFBW, samples with PhE presented a decrease in protein dissolution. Thermal treatment resulted in the disintegration of collagen fibrils and fibril bundles in SFBW samples, while the density of collagen fibrils was increased, and the porosity decreased in samples with PhE. The results of FTIR and intrinsic tryptophan fluorescence confirmed that the structures of SFBW were modified by PhE. Besides, the denaturing temperature and decomposition temperature were both improved with the addition of PhE. These results suggested that PhE appeared to have a positive effect on lowering oxidation and improving thermostability and structural stability of SFBW, which could provide a theoretical basis for protecting sea cucumber body wall against degradation during thermal tenderization.

DOI： 10.1016/j.foodres.2022.112419

第65回大会特別講演ポリフェノールの機能性の分子メカニズム : ポリフェノールはどのようにして機能性を発揮するのか—Molecular mechanisms for polyphenol functionality : How do polyphenols exert their functionality? 招待査読

中村宜督

日本食生活学会誌 = Journal for the integrated study of dietary habits / 日本食生活学会編 33 ( 4 ) 165 - 169 2023年

Construction of benzyl isothiocyanate-loaded fish skin gelatin-luteolin compound emulsion delivery system, and its digestion and absorption characteristics. 査読国際誌

Zhiyu Liu, Songyi Lin, Wenwen Liu, Yoshimasa Nakamura, Yue Tang

Journal of the science of food and agriculture 103 ( 4 ) 1864 - 1873 2022年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Fish skin gelatin (FSG) and luteolin (LUT) were used as composite emulsifiers, and benzyl isothiocyanate (BITC) was used as a model of nutrient delivery to construct a stable emulsion. The storage stability of the FSG-LUT emulsion and its effect on BITC release were investigated both in vitro and ex vivo. RESULTS: LUT can quench FSG fluorophores statically and form a stable complex through hydrogen bonding and hydrophobic interactions. The FSG-LUT emulsion storage stability and embedding rate were higher than those of the FSG emulsion. The FSG-LUT emulsion microstructure was resistant to oral and gastric digestion, and the BITC retention rate and bioaccessibility were much higher than those of the FSG emulsion. Lastly, the ex-vivo everted gut sac of rat intestine study demonstrated that BITC showed the highest absorption in the ileum, and the FSG-LUT emulsion absorbed BITC and sustained a controlled release in a specific position. CONCLUSION: LUT could form stable complexes with FSG, which improved the stability and bioavailability of BITC in the FSG-LUT emulsion delivery system, and promoted further intestinal BITC absorption. This article is protected by copyright. All rights reserved.

DOI： 10.1002/jsfa.12411

Phlorotannin Extracts from Ascophyllum nodosum Inhibited Proteases Activities and Structural Changes from Apostichopus japonicus 査読

Yu Ming, Yingzhen Wang, Yuqianqian Xie, Chenghang Sun, Xiufang Dong, Yoshimasa Nakamura, Xing Chen, Xiuping Dong, Hang Qi

ACS Food Science & Technology 2 ( 10 ) 1586 - 1596 2022年10月

Effects of Domestic Cooking Methods on Physichochemical Properties, Bioactive Compounds and Antioxidant Activities of Vegetables: A Mini-Review 査読

Shan Jiang, Meiqi Yu, Pengfei Jiang, Yoshimasa Nakamura, Hang Qi

Food Reviews International 1 - 15 2022年10月

Immune State Conversion of the Mesenteric Lymph Node in a Mouse Breast Cancer Model. 査読国際誌

Tsukasa Shigehiro, Maho Ueno, Mayumi Kijihira, Ryotaro Takahashi, Chiho Umemura, Eman A Taha, Chisaki Kurosaka, Megumi Asayama, Hiroshi Murakami, Ayano Satoh, Yoshimasa Nakamura, Junichiro Futami, Junko Masuda

International journal of molecular sciences 23 ( 19 ) 2022年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

Secondary lymphoid tissues, such as the spleen and lymph nodes (LNs), contribute to breast cancer development and metastasis in both anti- and pro-tumoral directions. Although secondary lymphoid tissues have been extensively studied, very little is known about the immune conversion in mesenteric LNs (mLNs) during breast cancer development. Here, we demonstrate inflammatory immune conversion of mLNs in a metastatic 4T1 breast cancer model. Splenic T cells were significantly decreased and continuously suppressed IFN-γ production during tumor development, while myeloid-derived suppressor cells (MDSCs) were dramatically enriched. However, T cell numbers in the mLN did not decrease, and the MDSCs only moderately increased. T cells in the mLN exhibited conversion from a pro-inflammatory state with high IFN-γ expression to an anti-inflammatory state with high expression of IL-4 and IL-10 in early- to late-stages of breast cancer development. Interestingly, increased migration of CD103+CD11b+ dendritic cells (DCs) into the mLN, along with increased (1→3)-β-D-glucan levels in serum, was observed even in late-stage breast cancer. This suggests that CD103+CD11b+ DCs could prime cancer-reactive T cells. Together, the data indicate that the mLN is an important lymphoid tissue contributing to breast cancer development.

DOI： 10.3390/ijms231911035

Extracellular malate induces stomatal closure via direct activation of guard-cell anion channel SLAC1 and stimulation of Ca2+ signaling. 査読国際誌

Yoshiharu Mimata, Shintaro Munemasa, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata

The New phytologist 2022年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Plants secrete malate from guard cells to apoplast under stress conditions and exogenous malate induces stomatal closure. Malate is considered as an extracellular chemical signal of stomatal closure. However, the molecular mechanism of malate-induced stomatal closure is not fully elucidated. We investigated responses of stomatal aperture, ion channels, and cytosolic Ca2+ to malate. A treatment with malate induced stomatal closure in Arabidopsis thaliana wild-type plants but not in the mutants deficient in the slow (S-type) anion channel gene SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1). The treatment with malate increased S-type anion currents in guard-cell protoplasts of wild-type plants but not in the slac1 mutant. In addition, extracellular rather than intracellular application of malate increased the S-type currents of constitutively active mutants of SLAC1, which have kinase-independent activities, in a heterologous expression system using Xenopus oocytes. The treatment with malate transiently increased cytosolic Ca2+ concentration in the wild-type Arabidopsis guard cells and the malate-induced stomatal closure was inhibited by the Ca2+ channel blocker and the Ca2+ chelator. These results indicate that extracellular malate directly activates SLAC1 and simultaneously stimulates Ca2+ signaling in guard cells, resulting in steady and solid activation of SLAC1 for stomatal closure.

DOI： 10.1111/nph.18400

Negative regulation of salicylic acid-induced stomatal closure by glutathione in Arabidopsis thaliana. 査読国際誌

Fahmida Akter, Shintaro Munemasa, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 2022年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Salicylic acid (SA) is a ubiquitous phenolic phytohormone that induces stomatal closure. Glutathione (GSH) negatively regulates stomatal closure induced by other plant hormones such as abscisic acid (ABA) and methyl jasmonate (MeJA). However, the involvement of GSH in SA-induced stomatal closure is still unknown. We investigated the regulation of SA signaling by GSH in guard cells using an Arabidopsis thaliana mutant, cad2-1, which is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase. Application of SA decreased stomatal apertures with decreasing intracellular GSH level in guard cells. Decreasing GSH by the cad2-1 mutation and by a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene, enhanced the SA-induced stomatal closure. A treatment with glutathione monoethyl ester restored the GSH level in the cad2-1 guard cells and complemented the stomatal phenotype of the mutant. These results indicate that GSH negatively modulates SA-induced stomatal closure in A. thaliana.

DOI： 10.1093/bbb/zbac116

Malate induces stomatal closure via a receptor-like kinase GHR1- and reactive oxygen species-dependent pathway in Arabidopsis thaliana. 査読国際誌

Yoshiharu Mimata, Shintaro Munemasa, Fahmida Akter, Israt Jahan, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 2022年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

A primary metabolite malate is secreted from guard cells in response to the phytohormone abscisic acid (ABA) and elevated CO2. The secreted malate subsequently facilitates stomatal closure in plants. Here, we investigated the molecular mechanism of malate-induced stomatal closure using inhibitors and ABA signaling component mutants of Arabidopsis thaliana. Malate-induced stomatal closure was impaired by a protein kinase inhibitor, K252a, and also by the disruption of a receptor-like kinase GHR1, which mediates activation of calcium ion (Ca2+) channel by reactive oxygen species (ROS) in guard cells. Malate induced ROS production in guard cells while the malate-induced stomatal closure was impaired by a peroxidase inhibitor, salicylhydroxamic acid, but not by the disruption of NAD(P)H oxidases, RBOHD and RBOHF. The malate-induced stomatal closure was impaired by Ca2+ channel blockers, verapamil and niflumic acid. These results demonstrate that the malate signaling is mediated by GHR1 and ROS in Arabidopsis guard cells.

DOI： 10.1093/bbb/zbac122

Modulating effects of oral administration of Lycii Fructus extracts on UVB-induced skin erythema: A Randomized, placebo-controlled study. 査読国際誌

Makoto Tsunenaga, Wensi Xu, Takumi Myojin, Toshiyuki Nakamura, Tatsuya Kon, Yoshimasa Nakamura, Osamu Ueda

Biomedical reports 17 ( 1 ) 62 - 62 2022年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Severe UV exposure induces skin inflammation, causing erythema. Lycii Fructus (Lycium barbarum and Lycium chinense) is a potential antioxidant agent with a high content of polyphenols, including rutin and chlorogenic acid. This study examined the effects of Lycii Fructus extract (LFE) on UVB-induced skin erythema in humans. Healthy volunteers were randomly assigned to one of two groups and received UVB irradiation at 1.5 minimal erythemal dose (MED) on day 0 at three designated sites on their backs, and the skin color was measured until day 7. After an 8-week treatment with LFE (900 mg/day) or placebo, UVB irradiation (l.5 MED) was applied again at different sites on day 63. Skin color was continuously measured in each group until day 69. LFE tablet administration for 8 weeks significantly inhibited UVB-induced erythema formation and increased the MED by 13%. Erythema formation peaked on the first day after UVB irradiation, but gradually dissipated over the next several days. LFE tended to accelerate erythema disappearance. To determine the polyphenol responsible for the protection against UVB-induced skin damage, the effects of LFE-derived polyphenols and their metabolites on UVB-induced cytotoxicity were examined in vitro. The major intestinal metabolite of rutin and LFE significantly attenuated phototoxicity and in human keratinocyte HaCaT cells. Quercetin enhanced intracellular glutathione levels in HaCaT cells, even though LFE did not increase it. Together, the results showed that LFE inhibited erythema formation and accelerated erythema dissipation, possibly through its direct antioxidative action.

DOI： 10.3892/br.2022.1545

過熱水蒸気処理玄米の食味,物性および抗酸化活性の評価—Evaluation of sensory properties, physical characteristics and antioxidant activity of superheated steam-treated brown rice 査読

西本有紀, 矢野愛奈, Wu Hongyan, 西澤正人, 中村俊之, 藤田明子, 中村宜督

美味技術学会誌 = Journal of the Japanese Society of Taste Technology 21 ( 1 ) 20 - 26 2022年7月

Antioxidative Effect of Chlorella Pyrenoidosa Protein Hydrolysates and Their Application in Krill Oil-in-Water Emulsions. 査読国際誌

Yujia Liu, Yuli Qi, Qi Wang, Fawen Yin, Honglei Zhan, Han Wang, Bingnan Liu, Yoshimasa Nakamura, Jihui Wang

Marine drugs 20 ( 6 ) 2022年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Chlorella pyrenoidosa is an excellent source of protein, and in this research, we assessed the antioxidant and emulsifying effects of Chlorella protein hydrolysate (CPH) using neutral proteases and alkaline proteases, as well as the properties of CPH-derived krill oil-in-water (O/W) emulsions. The CPHs exhibited the ability to scavenge several kinds of free radicals, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), O2-, hydroxyl, and ABTS. Additionally, the CPHs (5 mg/mL) scavenged approximately 100% of the DPPH and ABTS. The CPHs showed similar emulsifying activities to Tween 20 and excellent foaming activities (max FS 74%), which helped to stabilize the krill oil-in-water emulsion. Less than 10 mg/mL CPHs was able to form fresh krill oil-in-water emulsions; moreover, the CPHs (5 mg/mL) in a krill O/W emulsion were homogenous, opaque, and stable for at least 30 days. Based on their inhibitory effects on the peroxide value (POV) and thiobarbituric acid reactive substances (TRABS), the CPHs were found to be able to inhibit lipid oxidation in both emulsifying systems and krill O/W emulsions. Thus, the CPHs could improve superoxide dismutase (SOD) activities by 5- or 10-fold and decrease the high reactive oxygen species (ROS) level caused by the addition of H2O2 in vitro. In conclusion, health-promoting CPHs could be applied in krill oil-in-water emulsions as both emulsifiers and antioxidants, which could help to improve the oxidative and physical stability of emulsions.

DOI： 10.3390/md20060345

Influence of fish skin gelatin-sodium alginate complex stabilized emulsion on benzyl isothiocyanate stability and digestibility in vitro and in vivo. 査読国際誌

Xiangyu Zhang, Zhiyu Liu, Ailin Wang, Simin Zhang, Yoshimasa Nakamura, Songyi Lin, Yue Tang

Journal of the science of food and agriculture 2022年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: An emulsion delivery system for benzyl isothiocyanate (BITC) was prepared using fish skin gelatin (FSG) and sodium alginate (Alg). The effects of the FSG-Alg complex on the emulsion stability and BITC release pattern from the emulsion were investigated in vitro and in vivo. RESULTS: The storage stability and embedding rate of the 10 g/kg FSG and 2.5 g/kg Alg (FSG-Alg) emulsion were the highest among all samples. The FSG-Alg complex provided BITC a better protection during in vitro digestion. The microstructure of the FSG-Alg emulsions was more stable during in vitro digestion, and the bioaccessibility and retention rate of BITC were much higher compared to those of the FSG emulsion. The results of the ex-vivo everted gut sac of rat intestine study showed that the FSG-Alg emulsion significantly increased the BITC absorption rate in the duodenum. CONCLUSION: The FSG-Alg emulsion delivery system is a highly stable system for the delivery of BITC that improves the bioaccessibility of BITC and promotes its absorption in the duodenum. This article is protected by copyright. All rights reserved.

DOI： 10.1002/jsfa.11915

RING FISSION CATABOLITES OF QUERCETIN GLYCOSIDES

LI Kexin, NAKAMURA Toshiyuki, NAKAMURA Yoshimasa

Journal of Environmental Science for Sustainable Society 11 ( Supplement ) MR02_p5 - MR02_p8 2022年4月

Characterization of a synthetic zinc-chelating peptide from sea cucumber (Stichopus japonicus) and its gastrointestinal digestion and absorption in vitro. 査読国際誌

Zixu Wang, Jiatong Sun, Xiaoyu Ma, Xiaoyang Liu, Fawen Yin, Deyang Li, Yoshimasa Nakamura, Chenxu Yu, Dayong Zhou

Journal of the science of food and agriculture 2022年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Zinc absorption in intestinal system could be strongly affected by the gastrointestinal digestion and absorption of zinc-chelating peptides serving as zinc carriers. In this study, a novel zinc-chelating sea cucumber synthetic peptide (SCSP) was synthesized to estimate its gastrointestinal digestion and promotive effect of zinc absorption in vitro. RESULTS: Analysis of isothermal titration calorimetry suggested that the binding of SCSP and zinc (N ≈ 1) was exothermic, with relatively weak binding affinity (K = 1.0х10-3 mol L-1 ). The formation of SCSP-Zn complexes brought morphological changes to the peptides confirmed by scanning electron microscopy (SEM), which also indicated 6.88% of the existence of zinc element. In addition, the SCSP-Zn complexes remained stable under simulated human gastrointestinal digestion. In vitro study, the SCSP-Zn complex could successfully transport through the intestinal membrane in the model of everted rat gut sacs (nearly 7.5 μM cm-2 ) as well as Caco-2 cells where the zinc transport reached 0.0014 mg mL-1 carried by SCSP. Fluorescence staining experiments revealed free zinc accumulation inside the tissues and cells treated with the SCSP-Zn. CONCLUSIONS: The chelation SCSP-Zn had the promotion ability of zinc absorption in vitro and ex vivo experiments, which suggested a theoretical basis for the design and production of effective zinc chelating peptides as zinc carriers to improve zinc bioavailability. This article is protected by copyright. All rights reserved.

DOI： 10.1002/jsfa.11811

Gastrointestinal digestion and absorption characterization in vitro of zinc-chelating hydrolysate from scallop adductor (Patinopecten yessoensis). 査読国際誌

Jiatong Sun, Xiaoyang Liu, Zixu Wang, Fawen Yin, Huilin Liu, Yoshimasa Nakamura, Chenxu Yu, Dayong Zhou

Journal of the science of food and agriculture 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUD: Zinc is an essential catalytic element in human health system but its absorption in intestinal system could be strongly affected by the gastrointestinal digestion. In this study, the food-derived potential zinc carrier, scallop adductor hydrolysates (SAHs), was produced and characterized. RESULTS: During the temporary storage in 4°C, the SAH decreased in zinc-chelating capacity in aqueous phase whereas the SAH-Zn complex exhibited high stability. Moreover, the secondary structure of SAH had no significant alteration. Zinc morphologically altered the surface structures of SAH, which was involving in carboxyl group of SAH. Results of in vitro gastrointestinal (GI) digestion suggested that the SAH-Zn maintained good stability in GI system and only proportion of high molecular weight cleaved. In addition, SAH could successfully carry and transport zinc while the fluorescence staining revealed free zinc accumulation inside the tissue. Finally, 3 representative absorbed peptides (around 600 Da) were identified and synthesized. Three synthetic peptides exhibit higher zinc-chelating capacity than SAH and could also successfully transported through the intestine. CONCLUSION: This study provided a theoretical basis for the investigation of digestion and absorption of marine animal-derived peptides as zinc carriers. This article is protected by copyright. All rights reserved.

DOI： 10.1002/jsfa.11673

Neither glutamate nor alanine but arginine sensitizes BY-2 cells to arsenate. 査読国際誌

Nur-E-Nazmun Nahar, Md Yeasin Prodhan, Yoshiharu Mimata, Anna Yonezawa, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Arsenic is toxic for plants. Our previous results showed that the application of proline enhanced the sensitivity of tobacco BY-2 cells to arsenate. In order to clarify the enhancement mechanism, we investigated the effects of other amino acids on the arsenate-stressed BY-2 cells. Glutamate at up to 10 mM did not affect the cell growth in the absence or presence of arsenate. Arginine at up to 10 mM did not affect the growth in the absence of arsenate but arginine at 10 mM enhanced the inhibition of the cell growth by arsenate. Alanine at up to 10 mM did not affect the cell growth under non-stressed condition but alanine at 10 mM significantly improved the cell growth under arsenate stress. These results suggest that alanine mitigates arsenate stress in BY-2 cells and that arginine like proline enhances the sensitivity of BY-2 cells to arsenate.

DOI： 10.1093/bbb/zbab191

White rice ethanol extract is qualitatively, but not quantitatively, equivalent to that of brown rice as an antioxidant source. 査読国際誌

Hongyan Wu, Toshiyuki Nakamura, Yingnan Guo, Miho Hirooka, Gongliang Zhang, Shintaro Munemasa, Yoshiyuki Murata, Akiko Fujita, Yoshimasa Nakamura

Bioscience, biotechnology, and biochemistry 85 ( 10 ) 2161 - 2168 2021年9月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

The purpose of this study is to compare the potentials to exhibit biologically active antioxidant actions between white rice (WR) and brown rice (BR) in in vitro assays and a cellular model. The Trolox equivalent (TE) per 1 mg ethanol extract of WR for the 1,1-diphenyl-2-picrylhydrazyl assay was slightly higher than that of BR, whereas the TE per 1 g whole WR was much lower than that for BR. This tendency was very comparable to those for the oxygen radical absorbance capacity and total polyphenol content. Both of the ethanol extracts also similarly suppressed the hydrogen peroxide-induced cytotoxicity and enhanced the gene expression of drug-metabolizing enzymes. Based on the α-tocopherol quantity, its contribution to the cytoprotective effect of the rice extracts is very limited. Taken together, the ethanol extract of WR might be a qualitatively, but not quantitatively, equivalent source of antioxidative phytochemicals to that of BR.

DOI： 10.1093/bbb/zbab133

Cadmium uptake via apoplastic bypass flow in Oryza sativa. 査読

Izumi C Mori, Carlos Raul Arias-Barreiro, Lia Ooi, Nam-Hee Lee, Muhammad Abdus Sobahan, Yoshimasa Nakamura, Yoshihiko Hirai, Yoshiyuki Murata

Journal of plant research 134 ( 5 ) 1139 - 1148 2021年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

It is known that rice roots take up cadmium (Cd) via the symplastic route mediated by membrane-bound mineral transporters. Here we provide evidence that apoplastic bypass flow is another Cd uptake route in rice. High concentrations of Cd rendered apoplastic bypass flow rate increased in rice seedlings. These concentrations of Cd compromised membrane integrity in the root meristem and transition zone. Polyethleneglycol and proline inhibited the Cd-induced apoplastic bypass flow and Cd transfer to the shoots. Loss-of-function mutant of the Cd uptake transporter, nramp5, showed Cd transport to the shoot comparable to the wild type. At a low Cd concentration, increased apoplastic bypass flow rate by NaCl stress resulted in an elevation of Cd transport to shoots both in the wildtype and nramp5. These observations indicate that apoplastic bypass flow in roots carries Cd transport leading to xylem loading of Cd in addition to the symplastic pathway mediated by mineral transporters under stressed conditions.

DOI： 10.1007/s10265-021-01319-y

Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells. 査読国際誌

Md Tahjib-Ul-Arif, Shintaro Munemasa, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 85 ( 9 ) 2003 - 2010 2021年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mutants, slac1-4 slah3-3 and slac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cyt elevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cyt spike were lower and larger in the slac1-4 slah3-3 than in the wild type and the height and the number in the slac1-4 almt12-1 were much lower and much larger than in the wild type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cyt elevation in guard cells.

DOI： 10.1093/bbb/zbab118

A multidrug resistance-associated protein inhibitor is a potential enhancer of the benzyl isothiocyanate-induced apoptosis induction in human colorectal cancer cells. 査読国際誌

Qifu Yang, Toshiyuki Nakamura, Masayuki Seto, Miku Miyagawa, Wensi Xu, Beiwei Zhu, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

Journal of biochemical and molecular toxicology 35 ( 7 ) e22791 2021年7月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

The increasing drug efflux through the ATP-binding cassette (ABC) transporters is the most plausible mechanism that mediates resistance to the anticancer phytochemicals, such as benzyl isothiocyanate (BITC), as well as chemotherapy drugs. To identify a potential component to overcome this resistance by combinatory utilization, we focused on multidrug resistance-associated proteins (MRPs) pumping various drug metabolites with glutathione as well as the organic anions. The pharmacological treatment of an MRP inhibitor, MK571, significantly potentiated the BITC-induced antiproliferation, coincided with the enhanced accumulation of BITC and glutathione in human colorectal cancer HCT-116 cells. MK571 also enhanced the apoptosis induction as well as activation of the mitogen-activated protein kinases and caspase-3, whereas it did not affect their basal levels. These results suggested that, since MRPs might play a pivotal role in the BITC efflux, MK571 potentiates the BITC-induced antiproliferation in human colorectal cancer cells through inhibition of the glutathione-dependent BITC efflux.

DOI： 10.1002/jbt.22791

Luteolin suppresses 5-hydroxytryptamine elevation in stimulated RBL-2H3 cells and experimental colitis mice. 査読

Naoko Suga, Akira Murakami, Hideyuki Arimitsu, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoji Kato

Journal of clinical biochemistry and nutrition 69 ( 1 ) 20 - 27 2021年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Increased 5-hydroxytryptamine may be associated with the development and progression of inflammatory bowel disease. In this study, we examined the suppressive effect of flavonoids on the increased intra- and extracellular 5-hydroxytryptamine levels in rat mast RBL-2H3 cells, known to produce 5-hydroxytryptamine by the phorbol 12-myristate 13-acetate stimulation. Among the flavonoids examined, luteolin and quercetin significantly reduced the cellular 5-hydroxytryptamine concentration. Gene and protein expression analyses revealed that luteolin significantly suppressed cellular tryptophan hydroxylase 1 expression induced by phorbol 12-myristate 13-acetate stimulation. Mitogen-activated protein kinase/extracellular signal-regulated kinase signaling was also suppressed by luteolin, suggesting that this pathway is one of targets of 5-hydroxytryptamine modulation by luteolin. An in vivo experimental colitis model was prepared by administering 2.5% dextran sodium sulfate in drinking water to C57BL/6 mice for seven days. The ingestion of 0.1% dietary luteolin suppressed the increasing 5-hydroxytryptamine in the colorectal mucosa. In conclusion, luteolin possesses a suppressive effect on extensive 5-hydroxytryptamine formation in both experimental RBL-2H3 cells and colitis models.

DOI： 10.3164/jcbn.20-192

Functional Evaluation of the Ethanol Extracts from Rosmarinus officinalis L. (Rosemary) 査読

Yamada Naofumi, Yamasaki Tsutomu, Yamawaki Kana, Nakagiri Minami, Ito Hideyuki, Nakamura Yoshimasa, Nakanishi Tohru

Journal of Hard Tissue Biology 30 ( 3 ) 291 - 296 2021年

記述言語：英語出版者・発行元：硬組織再生生物学会

Rosemary is used as an herb in cooking and aromatic oils, and has long been used as a medicinal herb because of its functional components. According to folklore, monks recommended a remedy of rosemary in alcohol ("Hungarian water") to a Hungarian queen suffering from limb numbness, resulting in instant recovery and rejuvenation. This is why the queen married a 20-year-old king of Holland while in her seventies. In order to clarify the "rejuvenation" mechanisms of traditional Hungarian water, we examined the anti-oxidant activity, anti-glycation activity, inhibition of melanin production and suppression of tumor growth of ethanol extracts of rosemary in this study. It was found that the ethanol extract had high anti-oxidant activity, high anti-glycation activity, high inhibition of melanin production and high suppression of tumor cell growth.

DOI： 10.2485/jhtb.30.291

PREDOMINANT RICE PHYTOCHEMICALS AND THEIR DISEASE-PREVENTIVE EFFECTS

WU Hongyan, NAKAMURA Toshiyuki, NAKAMURA Yoshimasa

Journal of Environmental Science for Sustainable Society (Web) 10 ( Supplement ) 1 - 4 2021年

Stomatal response to isothiocyanates in Arabidopsis thaliana. 査読国際誌

Sonya Afrin, Eiji Okuma, Md Tahjib-Ul-Arif, Md Sarwar Jahan, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

Journal of experimental botany 71 ( 22 ) 6921 - 6931 2020年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Allyl isothiocyanate (AITC) induces stomatal closure accompanied by reactive oxygen species (ROS) production and glutathione (GSH) depletion in Arabidopsis thaliana. In this study, stomatal responses to three other isothiocyanates (ITCs), benzyl isothiocyanate (BITC), sulforaphane (SFN), and phenethyl isothiocyanate (PEITC), were investigated in A. thaliana. All these ITCs significantly induced stomatal closure, where PEITC and BITC were most effective. The selected ITCs also induced ROS accumulation, cytosolic alkalization, and GSH depletion in guard cells. Moreover, all ITCs increased the frequency of cytosolic free calcium ([Ca2+]cyt) spikes (transient elevation), while PEITC and BITC showed the highest frequency. There was a strong positive correlation between the number of [Ca2+]cyt spikes per guard cell and the decrease in stomatal aperture. Both cytosolic alkalization and GSH content have a positive correlation with the decrease in stomatal aperture, but ROS production did not have a significant correlation with the decrease in stomatal apertures. These results indicate that the molecules with a functional ITC group induce stomatal closure that is accompanied by GSH depletion, cytosolic alkalization, [Ca2+]cyt spikes, and ROS production, and that the former three cellular events, rather than ROS production, are highly correlated with the decrease in stomatal aperture.

DOI： 10.1093/jxb/eraa420

Fucoxanthin activities motivate its nano/micro-encapsulation for food or nutraceutical application: a review. 査読国際誌

Chunyan Wang, Xing Chen, Yoshimasa Nakamura, Chenxu Yu, Hang Qi

Food & function 11 ( 11 ) 9338 - 9358 2020年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Fucoxanthin is a xanthophyll carotenoid abundant in marine brown algae. The potential therapeutic effects of fucoxanthin on tumor intervention have been well documented, which have aroused great interests in utilizing fucoxanthin in functional foods and nutraceuticals. However, the utilization of fucoxanthin as a nutraceutical in food and nutrient supplements is currently limited due to its low water solubility, poor stability, and limited bioaccessibility. Nano/micro-encapsulation is a technology that can overcome these challenges. A systematic review on the recent progresses in nano/micro-delivery systems to encapsulate fucoxanthin in foods or nutraceuticals is warranted. This article starts with a brief introduction of fucoxanthin and the challenges of oral delivery of fucoxanthin. Nano/micro-encapsulation technology is then covered, including materials and strategies for constructing the delivery system. Finally, future prospective has been discussed on properly designed oral delivery systems of fucoxanthin for managing cancer. Natural edible materials such as whey protein, casein, zein, gelatin, and starch have been successfully utilized to fabricate lipid-based, gel-based, or emulsion-based delivery systems, molecular nanocomplexes, and biopolymer nanoparticles with the aid of advanced processing techniques, such as freeze-drying, high pressure homogenization, sonication, anti-solvent precipitation, coacervation, ion crosslinking, ionic gelation, emulsification, and enzymatic conjugation. These formulated nano/micro-capsules have proven to be effective in stabilizing and enhancing the bioaccessibility of fucoxanthin. This review will inspire a surge of multidisciplinary research in a broader community of foods and motivate material scientists and researchers to focus on nano/micro-encapsulated fucoxanthin in order to facilitate the commercialization of orally-deliverable tumor intervention products.

DOI： 10.1039/d0fo02176h

Exogenous proline enhances antioxidant enzyme activities but does not mitigate growth inhibition by selenate stress in tobacco BY-2 cells. 査読国際誌

Mousumi Khatun, Daiki Matsushima, Mohammad Saidur Rhaman, Eiji Okuma, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 84 ( 11 ) 2281 - 2292 2020年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

Selenium (Se) causes oxidative damage to plants. Proline is accumulated as a compatible solute in plants under stress conditions and mitigates stresses. Selenate at 250 µM increased cell death and inhibited the growth of tobacco BY-2 cells while exogenous proline at 10 mM did not mitigate the inhibition by selenate. Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells. Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells. Glutathione (GSH) rather than ascorbic acid (AsA) mitigated the growth inhibition although both reduced the accumulation of ROS induced by selenate. These results indicate that proline increases both antioxidant enzyme activities and Se accumulation, which overall fails to ameliorate the growth inhibition by selenate and that the growth inhibition is not accounted for only by ROS accumulation. Abbreviations: APX: ascorbate peroxidase; AsA: ascorbic acid; BY-2: Bright Yellow-2; CAT: catalase; DAI: days after inoculation; DW: dry weight; FW: fresh weight; GSH: glutathione; ROS: reactive oxygen species.

DOI： 10.1080/09168451.2020.1799747

Inhibition of Drug Resistance Mechanisms Improves the Benzyl Isothiocyanate–Induced Anti-Proliferation in Human Colorectal Cancer Cells 招待査読

Xiaoyang Liu, Qifu Yang, Yoshimasa Nakamura

Current Pharmacology Reports 6 ( 5 ) 306 - 314 2020年10月

Reactive carbonyl species mediate methyl jasmonate-induced stomatal closure. 査読

Md Moshiul Islam, Wenxiu Ye, Fahmida Akter, Mohammad Saidur Rhaman, Daiki Matsushima, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md Sanaullah Biswas, Jun'ichi Mano, Yoshiyuki Murata

Plant & cell physiology 61 ( 10 ) 1788 - 1797 2020年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Production of reactive oxygen species (ROS) is a key signal event for methyl jasmonate (MeJA)- and abscisic acid (ABA)-induced stomatal closure. We recently showed that reactive carbonyl species (RCS) stimulates stomatal closure as an intermediate downstream of hydrogen peroxide (H2O2) production in the ABA signaling pathway in guard cells of Nicotiana tabacum and Arabidopsis thaliana. In this study, we examined whether RCS functions as an intermediate downstream of H2O2 production in MeJA signaling in guard cells using transgenic tobacco plants overexpressing A. thaliana 2-alkenal reductase (n-alkanal + NAD(P)+ ⇌ 2-alkenal + NAD(P)H + H+) (AER-OE tobacco) and Arabidopsis plants. The stomatal closure induced by MeJA was impaired in the AER-OE tobacco and was inhibited by RCS scavengers, carnosine and pyridoxamine, in the wild-type (WT) tobacco plants and Arabidopsis plants. Application of MeJA significantly induced accumulation of RCS including acrolein and 4-hydroxy-(E)-2-nonenal in the WT tobacco but not in the AER-OE plants. Application of MeJA induced H2O2 production in the WT tobacco and the AER-OE plants and the H2O2 production was not inhibited by the RCS scavengers. These results suggest that RCS functions as an intermediate downstream of ROS production in MeJA signaling as well as in ABA signaling in guard cells.

DOI： 10.1093/pcp/pcaa107

Interaction of intracellular hydrogen peroxide accumulation with nitric oxide production in abscisic acid signaling in guard cells. 査読国際誌

Rayhanur Jannat, Takanori Senba, Daichi Muroyama, Misugi Uraji, Mohammad Anowar Hossain, Mohammad Muzahidul Islam, Yoshimasa Nakamura, Shintaro Munemasa, Izumi C Mori, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 84 ( 7 ) 1418 - 1426 2020年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Reactive oxygen species and nitric oxide (NO•) concomitantly play essential roles in guard cell signaling. Studies using catalase mutants have revealed that the inducible and constitutive elevations of intracellular hydrogen peroxide (H2O2) have different roles: only the inducible H2O2 production transduces the abscisic acid (ABA) signal leading stomatal closure. However, the involvement of inducible or constitutive NO• productions, if exists, in this process remains unknown. We studied H2O2 and NO• mobilization in guard cells of catalase mutants. Constitutive H2O2 level was higher in the mutants than that in wild type, but constitutive NO• level was not different among lines. Induced NO• and H2O2 levels elicited by ABA showed a high correlation with each other in all lines. Furthermore, NO• levels increased by exogenous H2O2 also showed a high correlation with stomatal aperture size. Our results demonstrate that ABA-induced intracellular H2O2 accumulation triggers NO• production leading stomatal closure. ABBREVIATIONS: ABA: abscisic acid; CAT: catalase; cGMP: cyclic guanosine monophosphate; DAF-2DA: 4,5-diaminofluorescein-2 diacetate; H2DCF-DA: 2',7'-dichlorodihydrofluorescein diacetate; MeJA: methyljasmonate; NOS: nitric oxide synthetase; NR: nitrate reductase; POX: peroxidase; ROS: reactive oxygen species; SNAP: S-nitroso-N-acetyl-DL-penicillamine; SNP: sodium nitroprusside; NOX: NADP(H) oxidase.

DOI： 10.1080/09168451.2020.1743168

Inhibition of light-induced stomatal opening by allyl isothiocyanate does not require guard cell cytosolic Ca2+ signaling. 査読国際誌

Wenxiu Ye, Eigo Ando, Mohammad Saidur Rhaman, Md Tahjib-Ul-Arif, Eiji Okuma, Yoshimasa Nakamura, Toshinori Kinoshita, Yoshiyuki Murata

Journal of experimental botany 71 ( 10 ) 2922 - 2932 2020年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

The glucosinolate-myrosinase system is a well-known defense system that has been shown to induce stomatal closure in Brassicales. Isothiocyanates are highly reactive hydrolysates of glucosinolates, and an isothiocyanate, allyl isothiocyanate (AITC), induces stomatal closure accompanied by elevation of free cytosolic Ca2+ concentration ([Ca2+]cyt) in Arabidopsis. It remains unknown whether AITC inhibits light-induced stomatal opening. This study investigated the role of Ca2+ in AITC-induced stomatal closure and inhibition of light-induced stomatal opening. AITC induced stomatal closure and inhibited light-induced stomatal opening in a dose-dependent manner. A Ca2+ channel inhibitor, La3+, a Ca2+chelator, EGTA, and an inhibitor of Ca2+ release from internal stores, nicotinamide, inhibited AITC-induced [Ca2+]cyt elevation and stomatal closure, but did not affect inhibition of light-induced stomatal opening. AITC activated non-selective Ca2+-permeable cation channels and inhibited inward-rectifying K+ (K+in) channels in a Ca2+-independent manner. AITC also inhibited stomatal opening induced by fusicoccin, a plasma membrane H+-ATPase activator, but had no significant effect on fusicoccin-induced phosphorylation of the penultimate threonine of H+-ATPase. Taken together, these results suggest that AITC induces Ca2+ influx and Ca2+ release to elevate [Ca2+]cyt, which is essential for AITC-induced stomatal closure but not for inhibition of K+in channels and light-induced stomatal opening.

DOI： 10.1093/jxb/eraa073

The Myrosinases TGG1 and TGG2 Function Redundantly in Reactive Carbonyl Species Signaling in Arabidopsis Guard Cells. 査読

Mohammad Saidur Rhaman, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

Plant & cell physiology 61 ( 5 ) 967 - 977 2020年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Myrosinase (β-thioglucoside glucohydrolase, enzyme nomenclature, EC 3.2.1.147, TGG) is a highly abundant protein in Arabidopsis guard cells, of which TGG1 and TGG2 function redundantly in abscisic acid (ABA)- and methyl jasmonate-induced stomatal closure. Reactive carbonyl species (RCS) are α,β-unsaturated aldehydes and ketones, which function downstream of reactive oxygen species (ROS) production in the ABA signalling pathway in guard cells. Among the RCS, acrolein is the most highly reactive, which is significantly produced in ABA-treated guard cells. To clarify the ABA signal pathway downstream of ROS production, we investigated the responses of tgg mutants (tgg1-3, tgg2-1 and tgg1-3 tgg2-1) to acrolein. Acrolein induced stomatal closure and triggered cytosolic alkalization in wild type (WT), tgg1-3 single mutants and in tgg2-1 single mutants, but not in tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ induced stomatal closure and cytosolic alkalization not only in WT but also in all of the mutants. Acrolein- and Ca2+-induced stomatal closures were inhibited by an intracellular acidifying agent, butyrate, a Ca2+ chelator, ethylene glycol tetraacetic acid (EGTA) and a Ca2+ channel blocker, LaCl3. Acrolein induced cytosolic free calcium concentration ([Ca2+]cyt) elevation in guard cells of WT plants but not in the tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ elicited [Ca2+]cyt elevation in guard cells of WT and tgg1-3 tgg2-1. Our results suggest that TGG1 and TGG2 function redundantly, not between ROS production and RCS production, but downstream of RCS production in the ABA signal pathway in Arabidopsis guard cells.

DOI： 10.1093/pcp/pcaa024

Salicylic acid receptor NPR1 is involved in guard cell chitosan signaling. 査読国際誌

Yeasin Prodhan, Mohammad Issak, Shintaro Munemasa, Yoshimasa Nakamura, Yoshiyuki Murata

Bioscience, biotechnology, and biochemistry 84 ( 5 ) 963 - 969 2020年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

Chitosan (CHT) induces stomatal closure and thus plays a crucial role in plants to adapt to the adverse environments. Our previous results of a SA-deficient mutant nahG suggest that endogenous salicylic acid (SA) is involved in the CHT signaling in guard cells. Here in order to make the involvement definite, we examined stomatal responses to CHT of another SA-deficient mutant, sid2, and an SA receptor mutant, npr1-3. The sid2 mutation impaired CHT-induced stomatal closure and reactive oxygen species production and both impairments were complemented with exogenous SA application. Moreover, the CHT-induced stomatal closure is disrupted in the npr1-3 mutant. These results suggest that endogenous SA is involved in the CHT-induced stomatal closure via the SA receptor, NPR1.Abbreviations: SA: salicylic acid; ABA: abscisic acid; ROS: reactive oxygen species; NPR1: nonexpresser of pathogenesis-related genes1; CHT: chitosan; DAB: 3,3'-diaminobenzidine.

DOI： 10.1080/09168451.2020.1718485

Synthesis and characterization of conductive flexible cellulose carbon nanohorn sheets for human tissue applications. 査読国際誌

Karthik Paneer Selvam, Taichi Nagahata, Kosuke Kato, Mayuko Koreishi, Toshiyuki Nakamura, Yoshimasa Nakamura, Takeshi Nishikawa, Ayano Satoh, Yasuhiko Hayashi

Biomaterials research 24 18 - 18 2020年

記述言語：英語掲載種別：研究論文（学術雑誌）

Background: Conductive sheets of cellulose and carbon nanomaterials and its human skin applications are an interesting research aspect as they have potential for applications for skin compatibility. Hence it is needed to explore the effects and shed light on these applications. Method: To fabricate wearable, portable, flexible, lightweight, inexpensive, and biocompatible composite materials, carbon nanohorns (CNHs) and hydroxyethylcellulose (HEC) were used as precursors to prepare CNH-HEC (Cnh-cel) composite sheets. Cnh-cel sheets were prepared with different loading concentrations of CNHs (10, 20 50,100 mg) in 200 mg cellulose. To fabricate the bio-compatible sheets, a pristine composite of CNHs and HEC was prepared without any pretreatment of the materials. Results: The obtained sheets possess a conductivity of 1.83 × 10- 10 S/m and bio-compatible with human skin. Analysis for skin-compatibility was performed for Cnh-cel sheets by h-CLAT in vitro skin sensitization tests to evaluate the activation of THP-1 cells. It was found that THP-1 cells were not activated by Cnh-cel; hence Cnh-cel is a safe biomaterial for human skin. It was also found that the composite allowed only a maximum loading of 100 mg to retain the consistent geometry of free-standing sheets of < 100 μm thickness. Since CNHs have a unique arrangement of aggregates (dahlia structure), the composite is homogeneous, as verified by transmission electron microscopy (TEM) and, scanning electron microscopy (SEM), and other functional properties investigated by Raman spectroscopy, Fourier transform infrared spectroscopy (FT-IR), conductivity measurement, tensile strength measurement, and skin sensitization. Conclusion: It can be concluded that cellulose and CNHs sheets are conductive and compatible to human skin applications.

DOI： 10.1186/s40824-020-00194-3

Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate. 査読

Liu X, Wang Z, Zhang J, Song L, Li D, Wu Z, Zhu B, Nakamura Y, Shahidi F, Yu C, Zhou D

Journal of the science of food and agriculture 99 ( 14 ) 6400 - 6407 2019年11月

Dynamics of the Cellular Metabolism of Leptosperin Found in Manuka Honey. 査読国際誌

Yoji Kato, Masaki Kawai, Shota Kawai, Yayako Okano, Natsumi Rokkaku, Akari Ishisaka, Kaeko Murota, Toshiyuki Nakamura, Yoshimasa Nakamura, Shinichi Ikushiro

Journal of agricultural and food chemistry 67 ( 39 ) 10853 - 10862 2019年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Leptosperin (methyl syringate β-d-gentiobioside) is abundantly found in manuka honey, which is widely used because of its antibacterial and possible anti-inflammatory activities. The aim of this study was to examine the molecular mechanism underlying the metabolism of leptosperin. Five phytochemicals (leptosperin, methyl syringate (MSYR), glucuronate conjugate of MSYR (MSYR-GA), sulfonate conjugate of MSYR (MSYR-S), and syringic acid (SYR)) were separately incubated with HepG2 and Caco-2 cells. After incubation, we found that the concentration of MSYR decreased, whereas the concentrations of SYR, MSYR-GA, and MSYR-S increased. By profiling with inhibitors and carboxylesterases (CES1, 2), we found that the conversion from MSYR to SYR was mediated by CES1. Lipopolysaccharide-stimulated RAW264.7 cells restored MSYR-GA to MSYR possibly by the secreted β-glucuronidase. All of the mice administered with leptosperin, MSYR, or manuka honey showed higher MSYR (13.84 ± 11.51, 14.29 ± 9.19, or 6.66 ± 2.30 nM) and SYR (1.85 ± 0.66, 6.01 ± 1.20, or 8.16 ± 3.10 nM) levels in the plasma compared with that of the vehicle controls (3.33 ± 1.45 (MSYR) and 1.85 ± 0.66 (SYR) nM). The findings of our study indicate that the unique metabolic pathways of these compounds may account for possible functionalities of manuka honey.

DOI： 10.1021/acs.jafc.9b03894

Methyl-β-cyclodextrin potentiates the BITC-induced anti-cancer effect through modulation of the Akt phosphorylation in human colorectal cancer cells. 査読

Ethylene Inhibits Methyl Jasmonate-Induced Stomatal Closure by Modulating Guard Cell Slow-Type Anion Channel Activity via the OPEN STOMATA 1/SnRK2.6 Kinase-Independent Pathway in Arabidopsis. 査読

Munemasa S, Hirao Y, Tanami K, Mimata Y, Nakamura Y, Murata Y

Plant & cell physiology 60 ( 10 ) 2263 - 2271 2019年10月

Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides 査読

Xiaoyang Liu, Zixu Wang, Fawen Yin, Yuxin Liu, Ningbo Qin, Yoshimasa Nakamura, Fereidoon Shahidi, Chenxu Yu, Dayong Zhou, Beiwei Zhu

Marine Drugs 17 ( 8 ) 2019年7月

Reactive carbonyl species function as signal mediators downstream of H<inf>2</inf>O<inf>2</inf> production and regulate [Ca²⁺]<inf>cyt</inf> elevation in ABA signal pathway in Arabidopsis guard cells 査読

Md Moshiul Islam, Wenxiu Ye, Daiki Matsushima, Mohammad Saidur Rhaman, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md Sanaullah Biswas, Jun'ichi Mano, Yoshiyuki Murata

Plant and Cell Physiology 60 ( 5 ) 1146 - 1159 2019年5月

Covalent modification of phosphatidylethanolamine by benzyl isothiocyanate and the resultant generation of ethanolamine adduct as its metabolite. 査読国際誌

Nakamura T, Hirakawa M, Nakamura Y, Ishisaka A, Kitamoto N, Murakami A, Kato Y

Chemical research in toxicology 32 ( 4 ) 638 - 644 2019年2月

The Golgin Protein Giantin Regulates Interconnections Between Golgi Stacks. 査読国際誌

Ayano Satoh, Mitsuko Hayashi-Nishino, Takuto Shakuno, Junko Masuda, Mayuko Koreishi, Runa Murakami, Yoshimasa Nakamura, Toshiyuki Nakamura, Naomi Abe-Kanoh, Yasuko Honjo, Joerg Malsam, Sidney Yu, Kunihiko Nishino

Frontiers in cell and developmental biology 7 ( AUG ) 160 - 160 2019年

Oxidative stress involved in textural changes of sea cucumber Stichopus japonicus body wall during low-temperature treatment. 査読

Dong X, Qi H, Feng D, He B, Nakamura Y, Yu C, Zhu B

Int. J. Food Prop. 21 ( 1 ) 2646 - 2659 2018年12月

Yang Q, Miyagawa M, Liu X, Zhu B, Munemasa S, Nakamura T, Murata Y, Nakamura Y

Bioscience, biotechnology, and biochemistry 82 ( 12 ) 2158 - 2167 2018年12月

Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. 査読

Liang Y, Sasaki I, Takeda Y, Zhu B, Munemasa S, Nakamura T, Murata Y, Nakamura Y

Bioscience, biotechnology, and biochemistry 82 ( 12 ) 2130 - 2139 2018年12月

Lycii fructus extract ameliorates hydrogen peroxide-induced cytotoxicity through indirect antioxidant action. 査読

Xu W, Saiki S, Myojin T, Liu Y, Zhu B, Murata Y, Ashida H, Tsunenaga M, Nakamura Y

Bioscience, biotechnology, and biochemistry 82 ( 10 ) 1812 - 1820 2018年10月

Guard Cell Salicylic Acid Signaling Is Integrated into Abscisic Acid Signaling via the Ca²⁺/CPK-Dependent Pathway. 査読

Prodhan MY, Munemasa S, Nahar MN, Nakamura Y, Murata Y

Plant physiology 178 ( 1 ) 441 - 450 2018年9月

Lymphatic metabolites of quercetin after intestinal administration of quercetin-3-glucoside and its aglycone in rats 査読

Toshiyuki Nakamura, Chinatsu Kinjo, Yoshimasa Nakamura, Yoji Kato, Miyu Nishikawa, Masahiro Hamada, Noriyuki Nakajima, Shinichi Ikushiro, Kaeko Murota

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 645 126 - 136 2018年5月

Benzyl isothiocyanate attenuates the hydrogen peroxide-induced interleukin-13 expression through glutathione S-transferase P induction in T lymphocytic leukemia cells. 査読

Tang Y, Naito S, Abe-Kanoh N, Ogawa S, Yamaguchi S, Zhu B, Murata Y, Nakamura Y

Journal of biochemical and molecular toxicology 32 ( 6 ) e22054 2018年4月

Phenethyl isothiocyanate activates leptin signaling and decreases food intake. 査読国際誌

Miho Yagi, Yukiko Nakatsuji, Ayumi Maeda, Hiroki Ota, Ryosuke Kamikubo, Noriyuki Miyoshi, Yoshimasa Nakamura, Mitsugu Akagawa

PloS one 13 ( 11 ) e0206748 2018年

記述言語：英語掲載種別：研究論文（学術雑誌）

Obesity, a principal risk factor for the development of diabetes mellitus, heart disease, and hypertension, is a growing and serious health problem all over the world. Leptin is a weight-reducing hormone produced by adipose tissue, which decreases food intake via hypothalamic leptin receptors (Ob-Rb) and the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway. Protein tyrosine phosphatase 1B (PTP1B) negatively regulates leptin signaling by dephosphorylating JAK2, and the increased activity of PTP1B is implicated in the pathogenesis of obesity. Hence, inhibition of PTP1B may help prevent and reduce obesity. In this study, we revealed that phenethyl isothiocyanate (PEITC), a naturally occurring isothiocyanate in certain cruciferous vegetables, potently inhibits recombinant PTP1B by binding to the reactive cysteinyl thiol. Moreover, we found that PEITC causes the ligand-independent phosphorylation of Ob-Rb, JAK2, and STAT3 by inhibiting cellular PTP1B in differentiated human SH-SY5Y neuronal cells. PEITC treatment also induced nuclear accumulation of phosphorylated STAT3, resulting in enhanced anorexigenic POMC expression and suppressed orexigenic NPY/AGRP expression. We demonstrated that oral administration of PEITC to mice significantly reduces food intake, and stimulates hypothalamic leptin signaling. Our results suggest that PEITC might help prevent and improve obesity.

DOI： 10.1371/journal.pone.0206748

Inhibition of phosphatidylinositide 3-kinase ameliorates antiproliferation by benzyl isothiocyanate in human colon cancer cells 査読

Xiaoyang Liu, Chiaki Takano, Tomomi Shimizu, Shintaro Yokobe, Naomi Abe-Kanoh, Beiwei Zhu, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 491 ( 1 ) 209 - 216 2017年9月

(−)-Epigallocatechin-3-gallate inhibits human angiotensin-converting enzyme activity through an autoxidation-dependent mechanism 査読

Zhe Liu, Sayaka Nakashima, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

Journal of Biochemical and Molecular Toxicology 31 ( 9 ) 2017年9月

Methylglyoxal induces inhibition of growth, accumulation of anthocyanin, and activation of glyoxalase I and II in Arabidopsis thaliana 査読

Tahsina Sharmin Hoque, Misugi Uraji, Md Anamul Hoque, Yoshimasa Nakamura, Yoshiyuki Murata

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 31 ( 7 ) 2017年7月

Cytotoxic and cytoprotective effects of tryptamine-4,5-dione on neuronal cells: a double-edged sword 査読

Naoko Suga, Akira Murakami, Yoshimasa Nakamura, Akari Ishisaka, Noritoshi Kitamoto, Mikiko Ito, Yoji Kato

FREE RADICAL RESEARCH 51 ( 5 ) 545 - 553 2017年

3,4-Dihydroxyphenylacetic acid is a potential aldehyde dehydrogenase inducer in murine hepatoma Hepa1c1c7 cells 査読

Yujia Liu, Ayuki Kurita, Sayaka Nakashima, Beiwei Zhu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 ( 10 ) 1978 - 1983 2017年

MPK9 and MPK12 function in SA-induced stomatal closure in Arabidopsis thaliana 査読

Md. Atiqur Rahman Khokon, Mohammad Abdus Salam, Fabien Jammes, Wenxiu Ye, Mohammad Anowar Hossain, Eiji Okuma, Yoshimasa Nakamura, Izumi C. Mori, June M. Kwak, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 ( 7 ) 1394 - 1400 2017年

Exogenous proline enhances the sensitivity of Tobacco BY-2 cells to arsenate 査読

Mst Nur-E-Nazmun Nahar, Mohammad Muzahidul Islam, Md. Anamul Hoque, Anna Yonezawa, Md. Yeasin Prodhan, Toshiyuki Nakamura, Yoshimasa Nakamura, Shintaro Munemasa, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 ( 9 ) 1726 - 1731 2017年

Chitosan signaling in guard cells requires endogenous salicylic acid 査読

Md. Yeasin Prodhan, Mohammad Issak, Toshiyuki Nakamura, Shintaro Munemasa, Yoshimasa Nakamura, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 ( 8 ) 1536 - 1541 2017年

Inhibition of phosphatidylinositide 3-kinase impairs the benzyl isothiocyanate-induced accumulation of autophagic molecules and Nrf2 in human colon cancer cells 査読

Xiaoyang Liu, Naomi Abe-Kanoh, Yujia Liu, Beiwei Zhu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 ( 11 ) 2212 - 2215 2017年

Reactive Carbonyl Species Mediate ABA Signaling in Guard Cells 査読

Md. Moshiul Islam, Wenxiu Ye, Daiki Matsushima, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md. Sanaullah Biswas, Jun'ichi Mano, Yoshiyuki Murata

PLANT AND CELL PHYSIOLOGY 57 ( 12 ) 2552 - 2563 2016年12月

Galloylated Catechins as Potent Inhibitors of Angiotensin Converting Enzyme 査読

Zhe Liu, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura

FOOD SCIENCE AND TECHNOLOGY RESEARCH 22 ( 6 ) 847 - 851 2016年11月

A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite 査読

Sayaka Nakashima, Zhe Liu, Yuya Yamaguchi, Shunya Saiki, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

Biochemistry and Biophysics Reports 7 240 - 245 2016年9月

Involvement of OST1 Protein Kinase and PYR/PYL/RCAR Receptors in Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis Guard Cells 査読

Ye Yin, Yuji Adachi, Yoshimasa Nakamura, Shintaro Munemasa, Izumi C. Mori, Yoshiyuki Murata

PLANT AND CELL PHYSIOLOGY 57 ( 8 ) 1779 - 1790 2016年8月

Apoptosis induction is involved in UVA-induced autolysis in sea cucumber Stichopus japonicus 査読

Hang Qi, Hui Fu, Xiufang Dong, Dingding Feng, Nan Li, Chengrong Wen, Yoshimasa Nakamura, Beiwei Zhu

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 158 130 - 135 2016年5月

A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation 査読

Takashi Asahi, Xiaohong Wu, Hiroshi Shimoda, Shinsuke Hisaka, Etsuko Harada, Tomomi Kanno, Yoshimasa Nakamura, Yoji Kato, Toshihiko Osawa

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 80 ( 2 ) 313 - 317 2016年2月

アブラナ科野菜漬物（カブ，ハクサイ）のイソチオシアネート生成に関する塩化ナトリウム（NaCl）およびアスコルビン酸の影響査読

宮澤紀子, 阿部雅子, 木村典代, 松岡寛樹, 田中進, 森光康次郎, 中村宜督, 綾部園子, 小澤好夫

日本調理科学会誌 49 ( 2 ) 138 - 146 2016年

記述言語：日本語出版者・発行元：一般社団法人日本調理科学会

野菜を常に摂取すると生活習慣病のリスクを軽減することが知られている。日本では，グルコシノレートを含む多くのアブラナ科野菜が漬物や煮物さらに浅漬けとして消費されている。グルコシノレートは組織が損傷するとミロシナーゼにより独特なフレーバーと発がん抑制作用を有するイソチオシアネートに加水分解される。 NaClとアスコルビン酸（VC）がミロシナーゼ活性に与える影響について検討した結果，1％NaClと0.1％VC濃度が最もミロシナーゼ活性を賦活した。一方，NaClとVCの共存においては，活性は抑制されたが，その抑制率はVC濃度が増加するに従い減少した。さらに，漬物中のイソチオシアネート生成へのNaClの影響を検討するために，1, 2および3％NaCl濃度のカブとハクサイの浅漬けを調製した。GC-MS分析により3－ブテニル，4－ペンテニル，フェネチルイソチオシアネートを検出した。これらは，カブ，ハクサイ共に3％NaCl濃度で最も多く生成し，0.4％VCの添加は，1.4～1.6倍イソチオシアネートの生成を増大させた。官能評価により2％NaCl濃度における浅漬けが好まれた。

DOI： 10.11402/cookeryscience.49.138

Plant Polyphenols as a Double-Edged Sword in Health Promotion : Lessons from the Experimental Models Using Simple Phenolic Acids 招待査読

Nakamura Yoshimasa

Agri-bioscience monographs AGBM 6 ( 1 ) 1 - 57 2016年

Specific role of taurine in the 8-brominated-2′-deoxyguanosine formation 査読

Takashi Asahi, Takashi Asahi, Yoshimasa Nakamura, Yoji Kato, Toshihiko Osawa

Archives of Biochemistry and Biophysics 586 45 - 50 2015年11月

Allyl isothiocyanate induces stomatal closure in Vicia faba 査読

Muhammad Abdus Sobahan, Nasima Akter, Eiji Okuma, Misugi Uraji, Wenxiu Ye, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 79 ( 10 ) 1737 - 1742 2015年10月

Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana 査読

Md. A. R. Khokon, M. A. Salam, F. Jammes, W. Ye, M. A. Hossain, M. Uraji, Y. Nakamura, I. C. Mori, J. M. Kwak, Y. Murata

PLANT BIOLOGY 17 ( 5 ) 946 - 952 2015年9月

Open Stomata 1 Kinase is Essential for Yeast Elicitor-Induced Stomatal Closure in Arabidopsis 査読

Wenxiu Ye, Yuji Adachi, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

PLANT AND CELL PHYSIOLOGY 56 ( 6 ) 1239 - 1248 2015年6月

Benzyl isothiocyanate inhibits IL-13 expression in human basophilic KU812 cells 査読

Yue Tang, Naomi Abe, Motoko Yoshimoto, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 79 ( 1 ) 159 - 163 2015年1月

Inhibition by acrolein of light-induced stomatal opening through inhibition of inward-rectifying potassium channels in Arabidopsis thaliana 査読

Md. Moshiul Islam, Wenxiu Ye, Daiki Matsushima, Md. Atiqur Rahman Khokon, Shintaro Munemasa, Yoshimasa Nakamura, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 79 ( 1 ) 59 - 62 2015年1月

Cyclic adenosine 5 '-diphosphoribose (cADPR) cyclic guanosine 3 ',5 '-monophosphate positively function in Ca2+ elevation in methyl jasmonate-induced stomatal closure, cADPR is required for methyl jasmonate-induced ROS accumulation NO production in guard cells 査読

M. A. Hossain, W. Ye, S. Munemasa, Y. Nakamura, I. C. Mori, Y. Murata

PLANT BIOLOGY 16 ( 6 ) 1140 - 1144 2014年11月

Tea Catechins Inhibit Cell Proliferation Through Hydrogen Peroxide-Dependent and -Independent Pathways in Human T lymphocytic Leukemia Jurkat Cells 査読

Yue Tang, Naomi Abe, Hang Qi, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

FOOD SCIENCE AND TECHNOLOGY RESEARCH 20 ( 6 ) 1245 - 1249 2014年11月

(-)-Epigallocatechin-3-Gallate Ameliorates Photodynamic Therapy Responses in an In Vitro T Lymphocyte Model 査読

Hang Qi, Naomi Abe, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

PHYTOTHERAPY RESEARCH 28 ( 10 ) 1486 - 1491 2014年10月

Thiol modification by bioactivated polyphenols and its potential role in skin inflammation 査読

Yoshimasa Nakamura, Takeshi Ishii, Naomi Abe, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 78 ( 6 ) 1067 - 1070 2014年6月

Ascorbic Acid Synergistically Potentiates Phloxine B-induced Photocytotoxicity in Human Acute Promyelocytic Leukemia Cells 査読

Hang Qi, Qian Wu, Naomi Abe, Shunya Saiki, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 28 ( 4 ) 167 - 173 2014年4月

Regulation of reactive oxygen species-mediated abscisic acid signaling in guard cells and drought tolerance by glutathione 査読

Shintaro Munemasa, Daichi Muroyama, Hiroki Nagahashi, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Frontiers in Plant Science 4 2013年11月

Erratum: Role of protein tyrosine phosphatase 1B in vascular endothelial growth factor signaling and cell-cell adhesions in endothelial cells (Circulation Research (2008) 102 (1182-1191) DOI: 10.1161/ CIRCRESAHA.107. 167080) 査読

Y. Nakamura, N. Patrushev, H. Inomata, D. Mehta, N. Urao, H. W. Kim, M. Razvi, V. Kini, K. Mahadev, B. J. Goldstein, R. McKinney, T. Fukai, M. Ushi-Fukai

Circulation Research 113 2013年10月

Calcium-dependent protein Kinase CPK6 positively functions in induction by yeast elicitor of stomatal closure and inhibition by yeast elicitor of light-induced stomatal opening in Arabidopsis 査読

Wenxiu Ye, Daichi Muroyama, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Plant Physiology 163 ( 2 ) 591 - 599 2013年10月

Difference in Abscisic Acid Perception Mechanisms between Closure Induction and Opening Inhibition of Stomata 査読

Ye Yin, Yuji Adachi, Wenxiu Ye, Maki Hayashi, Yoshimasa Nakamura, Toshinori Kinoshita, Izumi C. Mori, Yoshiyuki Murata

PLANT PHYSIOLOGY 163 ( 2 ) 600 - 610 2013年10月

Catalases CAT1 and CAT3 Are not Key Enzymes in Alleviating Gamma Irradiation-Induced DNA Damage, H2O2 Accumulation, or Lipid Peroxidation in Arabidopsis thaliana 査読

Amena Sultana, Ikuko Minami, Daiki Matsushima, Mohammad Issak, Yoshimasa Nakamura, Setsuko Todoriki, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 9 ) 1984 - 1987 2013年9月

Endogenous abscisic acid is involved in methyl jasmonate-induced reactive oxygen species and nitric oxide production but not in cytosolic alkalization in Arabidopsis guard cells 査読

Wenxiu Ye, Mohammad Anowar Hossain, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

JOURNAL OF PLANT PHYSIOLOGY 170 ( 13 ) 1212 - 1215 2013年9月

Lower Photostability of Capsanthin Dispersed in an Aqueous Solution 査読

Masayuki Nishino, Makoto Sakata, Yoshiyuki Murata, Yoshimasa Nakamura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 6 ) 1313 - 1316 2013年6月

Neither Endogenous Abscisic Acid nor Endogenous Jasmonate Is Involved in Salicylic Acid-, Yeast Elicitor-, or Chitosan-Induced Stomatal Closure in Arabidopsis thaliana 査読

Mohammad Issak, Eiji Okuma, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77 ( 5 ) 1111 - 1113 2013年5月

Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells 査読

M. A. Salam, F. Jammes, M. A. Hossain, W. Ye, Y. Nakamura, I. C. Mori, J. M. Kwak, Y. Murata

Plant Biology 15 ( 3 ) 436 - 442 2013年5月

Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis 査読

Nasima Akter, Eiji Okuma, Muhammad Abdus Sobahan, Misugi Uraji, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

JOURNAL OF PLANT GROWTH REGULATION 32 ( 1 ) 208 - 215 2013年3月

Effects of emulsifiers on the photostability of lycopene 査読

Masayuki Nishino, Makoto Sakata, Yoshiyuki Murata, Yoshimasa Nakamura

Food Science and Technology Research 19 ( 6 ) 983 - 987 2013年

Arabidopsis thalianaにおけるガンマ線照射誘導過酸化水素蓄積と脂質過酸化の軽減にカタラーゼCAT2は関与しない査読

Amena Sultana, 南育子, 松島大貴, Mohammad Issak, 中村宜督, 等々力節子, 村田芳行

食品照射 48 ( 1 ) 38 - 41 2013年

記述言語：日本語出版者・発行元：日本食品照射研究協議会

シロイヌナズナ野生株（Col-0）及びcat2変異体への10 kGyのガンマ線照射は，H2O2蓄積及び脂質過酸化生成を誘起したが，0.1 kGyと1 kGyのガンマ線照射は，誘起しなかった。また，野生株と変異体との間で，H2O2蓄積及び脂質過酸化に有意な差はなかった。以上の結果より，カタラーゼCAT2は，ガンマ線によって誘起される損傷を軽減するための重要な酵素ではないことが示唆された。

DOI： 10.5986/jrafi.48.38

CiNii Books

Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in arabidopsis thaliana 査読

Mohammad Shakhawat Hossain, Wenxiu Ye, Mohammad Anowar Hossain, Eiji Okuma, Misugi Uraji, Yoshimasa Nakamura, Izumi C. Mori, Yoshiyuki Murata

Bioscience, Biotechnology and Biochemistry 77 ( 5 ) 977 - 983 2013年

Tribolium castaneumの幼虫と成虫へのガンマ線の影響査読

Amena Sultana, 南育子, 市場隆二, Mohammad Issak, 多田幹郎, 中村宜督, 宮竹貴久, 等々力節子, 村田芳行

食品照射 48 ( 1 ) 19 - 23 2013年

記述言語：日本語出版者・発行元：日本食品照射研究協議会

ガンマ線を用いた食品照射は，穀物やスパイスの害虫の侵入を抑制する手段の一つである。我々は，Tribolium castaneumの幼虫と成虫へのガンマ線照射の影響を調べた。羽化は，500Gyの照射で完全に阻害された。成虫の生存率は，500Gy以上の照射で著しく減少した。中性条件でのコメントアッセイの結果より，500Gyまたは1000Gy照射によるDNA損傷は，不可逆的に増加したが，100Gy照射によるDNA損傷は一過的に増加した。100Gy照射ではDNAの修復が行われていることが示唆された。以上の結果より500Gy以上のガンマ線照射は，T. castaneum の幼虫と成虫の駆除に十分であることが示された。

DOI： 10.5986/jrafi.48.19

CiNii Books