Faculty Profiles - SAWA Yoshihiko

MISC - SAWA Yoshihiko

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ヒト歯根膜細胞が発現するコネキシンについて

山岡雄司, 沢禎彦, 吉田重光

解剖学雑誌 77 ( Suppl. ) APPENDIX23 - APPENDIX23 2002.3

ヒト歯根膜線維芽細胞のテロメア長

沢禎彦, 山岡雄司, 江畑典幸, 吉田重光

歯科基礎医学会雑誌 44 ( 5 ) 427 - 427 2002

口腔へん平上皮癌細胞株におけるconnexin 26およびconnexin43の検索

山岡雄司, 沢禎彦, 江畑典幸, 吉田重光

歯科基礎医学会雑誌 44 ( 5 ) 476 - 476 2002

ヒト歯髄線維芽細胞のCSF産生について

沢禎彦, 山岡雄司, 江畑典幸, 伊吹直子, 吉田重光

歯科基礎医学会雑誌 43 ( 5 ) 568 - 568 2001

培養ヒト歯根膜線維芽細胞が発現するconnexinの検索

山岡雄司, 沢禎彦, 伊吹直子, 江畑典幸, 吉田重光

歯科基礎医学会雑誌 43 ( 5 ) 571 - 571 2001

Human periodontal ligament fibroblasts express connexin 43

Y Yamaoka, Y Sawa, H Takagi, N Ebata, S Yoshida, T Kawasaki

JOURNAL OF DENTAL RESEARCH 79 ( 5 ) 1250 - 1250 2000.5

Immunohistochemical identification of lymphatic vessels by an antibody against desmoplakins

N Ebata, Y Sawa, Y Yamaoka, Y Totsuka

JOURNAL OF DENTAL RESEARCH 79 ( 5 ) 1247 - 1247 2000.5

The in vitro life-span of human periodontal ligament fibroblasts

Y Sawa, A Phillips, J Hollard, S Yoshida, MW Braithwaite

TISSUE & CELL 32 ( 2 ) 163 - 170 2000.4

Language：English Publisher：CHURCHILL LIVINGSTONE

The in vitro life-span of human periodontal ligament fibroblasts (PDLF) was studied on clones from periodontium of teeth extracted due to periodontitis and dental caries (69 clones/192 individuals, aged 20-80 years) and from periodontium of teeth extracted for orthodontic reasons (23 clones/26 individuals, aged 15-19 years). In the primary cultures the ratio of the number of cells expressing senescence-associated beta-galactosidase (SA-beta-Gal) to the total number of cells is significantly larger in PDLF (92 clones; 11.1-1-4.9%) than in human gingival fibroblasts IGF) (10 clones; 0.5+/-10.1%). The finite population doubling numbers (PD) of PDLF are not age-mutched and the mean PD of PDLF (7.1+/-2.9) is significantly smaller than GF (28.5+/-3.2), IMR-90 (human lung fibroblasts, 5 clones; 44.3 +/- 2.2), and human osteoblasts (5 clones, 19.7 +/- 1.4). Comparing the ratio of the number of SA-beta-Gal positive cells to the total number of cells in primary culture, and the finite PD in PDLF cultures: 1) the ratio of 15-19 years old donor group is significantly smaller than in the other donor groups (20-29, 30-39, 40-49, 50-59 and 60-80 years old), and 2) there were no statistically significant differences among the 20-29, 30-39, 40-49 and 5059 year old donor groups, and the 30-39, 40-49, 5059 and 60-80 year old donor groups. These findings suggest that the in vitro life-span of PDLF is shorter than other fibroblasts in the connective tissues and that PDLF may undergo senescence in adult clones without relation to donor's age, There may be more aged fibroblasts in periodontium than in other tissues, such as gingiva and lung. (C) 2000 Harcourt Publishers Ltd.

DOI： 10.1054/tice.2000.0100

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Impairment of osteocalcin production in senescent periodontal ligament fibroblasts

Y Sawa, A Phillips, J Hollard, S Yoshida, MW Braithwaite

TISSUE & CELL 32 ( 2 ) 198 - 204 2000.4

Language：English Publisher：CHURCHILL LIVINGSTONE

Osteocalcin production of senescent periodontal ligament fibroblasts (PDLF) with the expression of senescence-associated beta-galactosidase (SA-beta-Gal) was investigated on clones from 50-80 years old donors (n=20) with teeth extracted due to periodontitis and dental caries, and from 15-19 year old donors (n=20) with normal teeth extracted for orthodontic reasons. Immunohistochemically, the nonsenescent PDLF in all cultures in passage 2 showed strong reactivity with anti-osteocalcin. The reactive intensity of PDLF (passage 2, PD 3.0) was significantly stronger in 50-80 year old donor group than in 15-19 year old donor group, suggesting that osteocalcin production of PDLF cultured in early passage is larger in cells from adult population than in cells from adolescent population. In PDLF cultures in passage 2 from 50-80 year old donor, two types of senescent cells were found: one with strong reactivity to anti-osteocalcin and the other with little detectable reactivity. The culture consisted of senescent PDLF (passage 8, PD 14.8) did not include cells which have a detectable reactivity with anti-osteocalcin immunohistochemically and the reactive intensity was significantly weaker in the senescent culture than in the culture in passage 2 by ELISA, This suggests that the production potential of osteocalcin is impaired in PDLF with aging in culture. Further, the reactive intensity with anti-osteocalcin of PDLF in passage 2 deprived of serum for 48 h was 6% of that of cells cultured with serum and the reaction increased after serum stimulation, suggesting that the osteocalcin production in PDLF in early passage is implicated in mitogenic stimulation. (C) 2000 Harcourt Publishers Ltd.

DOI： 10.1054/tice.2000.0104

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Immuno-electron microscopic study of the expression of desmosome-associated proteins in cultured human periodontal ligament fibroblasts.

野田坂佳伸, 山岡雄司, 江畑典幸, 沢禎彦, 吉田重光

北海道歯学雑誌 21 ( 1 ) 130 - 135 2000