研究者詳細 - 田村　隆

A new selenoprotein from human lung adenocarcinoma cells: Purification, properties, and thioredoxin reductase activity

Takashi Tamura, Thressa C. Stadtman

Proceedings of the National Academy of Sciences of the United States of America 93 ( 3 ) 1006 - 1011 1996年2月

記述言語：英語

DOI： 10.1073/pnas.93.3.1006

Antimicrobial activity against methicillin-resistant Staphylococcus aureus in the culture broth of Enterococcus faecalis TH10, an isolate from Malaysian fermentation food, Temph

大平猪一朗, 田村隆, 藤井望, 稲垣賢ニ, 田中英彦

Jpn. J. Dairy Food Sci. 45(4), 93-96 ( 4 ) A - 93-A-96 1996年

記述言語：日本語出版者・発行元：日本酪農科学会

Bioassay-directed screening for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) led us to the culture broth of Enterococcus faecalis TH 10, which was isolated from a Malaysian fermentation food, Temph. The anti-MRSA component was readily extracted with ethyl acetate from the culture broth at pH 3, and the extract retained the activity when incubated overnight with various proteolytic enzymes. In addition, the extract showed poor antimicrobial activity against various lactic acid bacteria, including Enterococcus faecalis. These properties strongly suggest that the active compound is distinct from bacteriocin, an extracellularly released peptide or protein which shows potent activity against species closely related to the bacteriocin-producing strain. E. faecalis has been reported to produce bacteriocin which has strong hemolytic activity on mammalian erythrocytes, but the active component of the TH 10 strain did not mediate the lysis of rabbit and human erythrocytes.

DOI： 10.11465/milkscience.45.A-93

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722 好酸性細菌Acidobacterium capsulatum由来のキシラナーゼの精製及び諸性質の検討

その他リンク： https://jlc.jst.go.jp/DN/JLC/20016775976?from=CiNii

中平健, 向井和久, 田村隆, 稲垣賢二, 田中英彦

日本生物工学会大会講演要旨集 8 211 - 211 1996年

Antimicrobial activity against methicillin-resistant Staphylococcus aureus in the culture broth of Enterococcus faecalis TH10, an isolate from Malaysian fermentation food, Temph.

大平猪一朗, 田村隆, 藤井望, 稲垣賢ニ, 田中英彦

Japanese Journal of Dairy and Food Science 45 ( 4 ) 93 - 93-A-96 1996年

記述言語：日本語出版者・発行元：日本酪農科学会

DOI： 10.11465/milkscience.45.A-93

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<研究紹介>セレノシステイン含有ペプチドの合成と生化学的機能の研究

その他リンク： https://jlc.jst.go.jp/DN/JLC/20016775976?from=CiNii

微生物によるフッ素イオンの有機フッ素化合物への取り込み

江崎信芳, 田村隆, 和田大, 左右田健次

Biomedical research on trace elements 6 ( 3 ) 117 - 118 1995年12月

田村隆

岡山大学農学部学術報告 84 69 - 72 1995年2月

記述言語：日本語出版者・発行元：岡山大学

Selenium belongs to the Ⅵb group of the periodic table,and possesses both metallic and nonmetallic characteristics. Physicochemical properties of selenium resemble more or less those of sulfur,and selenium may be indiscriminately incorporated in place of sulfur in cellular constituents and disturb metabolism. Alkali disease and blind stagger disease of livestock are caused by selenium-polluted grass. Carcinogenic effect is also one of the marked biological properties of selenium. In spite of the toxic and carcinogenic effects,selenium is actually an essential trace element for bacteria,fish,and mammals. Life has exploied the high reactivity and unique characteristics of organoselenium compounds,especially in the form of selenoenzymes. Mammalian glutathione peroxidase(EC 1.11.1.9) has selenocysteine residue at the active site, and the enzyme plays a central role in the biological defense system against oxidative challenge by activated oxygens and radicals. The author has studied the low redox potential and high reactivity of selenium-containing compounds, and developed glutathione peroxidase mimics. Glutaselenone, a selenium analog of glutaheione, catalyzes a glutathione peroxidase like reaction in vitro. Studies on the mechanisum of glutaselenone-catalyzed reaction revealed that glutaselenone is converted to a selenosulfide conjugate with glutathione in its catalysis. Thioredoxins contain a conserved sequence,Cys-Gly-Pro-Cys, which is known to form intramolecular disulfide bond with consecutive β-turn conformations. THe peptide is expected to serve as an template for intramolecular selenosulfide bond formation when either the cysteine is replaced by selenocystein. A tetrapeptide,Secys-Gly-Pro-Cys, was synthesized chemically. It showed glutathione peroxidase-like activity three times as high as glutaselenone. The high catalytic activity is ascribed to an intramolecular selenosulfide bond formation in the catalytic reaction.セレンは2-、0、2+、4+、6+と多彩な酸化還元状態をとるという点で金属的な性質を持つ。また有機セレン化合物として求核的・求電子的な反応性に関与できるという点で酸素や硫黄などの典型元素のような振る舞いをする。生命は進化の過程で、セレンのユニークな化学的特性をうまく利用し、グルタチオンペルオキシダーゼなどのセレン酵素を獲得してきたと考えられる。セレン酵素の研究に触発されて、セレン酵素化合物を医薬品として利用する研究が進められており注目を集めている。このような背景のもと、グルタセレノンとテトラペプチドを合成した。とくにテトラペプチドはグルタセレノンの反応機構研究から生まれたペプチドである。今後さらに高い活性を持つ人工酵素がデザインされ、合成されるだろう。その時、鋳型として蛋白質や核酸、オリゴ糖などある特定のコンフォメーションをとる生体物質が利用されると思われる。

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その他リンク： http://ousar.lib.okayama-u.ac.jp/11140

Synthesis of Fluoroacetate from Fluoride, Glyceral, and β-Hydroxypyruvate by Streptomyces cattleya

Journal of Bacteriology 177 ( 9 ) 2265 - 2269 1995年

The mutual sparing effects of selenium and vitamin E in animal nutrition may be further explained by the discovery that mammalian thioredoxin reductase is a selenoenzyme

T Tamura, Gladyshev, V, SY Liu, TC Stadtman

BIOFACTORS 5 ( 2 ) 99 - 102 1995年

記述言語：英語掲載種別：書評論文，書評，文献紹介等出版者・発行元：IOS PRESS

The recent discovery that mammalian thioredoxin reductase is a selenoprotein furnishes an additional explanation of the mutual sparing roles of selenium and vitamin E in cellular antioxidant systems. Thioredoxin reductases isolated from human lung adenocarcinoma cells, human Jurkat T-cells and HeLa cells contain selenocysteine which is located in a C- terminal tripeptide, -Cys-SeCys-Gly.

Web of Science

Synthesis of fluoroacetate from fluoride, glycerol, and β- hydroxypyruvate by Streptomyces cattleya

T. Tamura, M. Wada, N. Esaki, K. Soda

Journal of Bacteriology 177 ( 9 ) 2265 - 2269 1995年

記述言語：英語

Streptomyces cattleya produces fluoroacetate and 4-fluorothreonine from inorganic fluoride added to the culture broth. We have shown by 19F nuclear magnetic resonance (NMR) spectrometry that fluoroacetate is accumulated first in the culture broth and that accumulation of 4-fluorothreonine is next. To show precursors of the carbon skeleton of fluoroacetate, we carried out tracer experiments with various 14C- and 13C-labeled compounds. Radioactivity of [U-14C]glucose, [U-14C]glycerol, [U-14C]serine, and [U-14C]β-hydroxypyruvate was incorporated into fluoroacetate to an extent of 0.2 to 0.4%, whereas [3-14C]pyruvate, [2,3-14C]succinate, and [U- 14C]aspartate were less efficiently incorporated (0.04 to 0.08%). The addition of [2-13C]glycerol to the mycelium suspension of Streptomyces cattleya caused exclusive enrichment of the carboxyl carbon of fluoroacetate with 13C
about 40% of carboxyl carbon of fluoroacetate was labeled with 13C. We studied the radioactivity incorporation of [3-14C]-, [U-14C]-, and [1-14C]β-hydroxypyruvates to show that C-2 and C-3 of β-hydroxy pyruvate are exclusively converted to the carbon skeleton of fluoroacetate. These results suggest that the carbon skeleton of fluoroacetate derives from C-1 and C-2 of glycerol through β-hydroxypyruvate, whose hydroxyl group is eventually replaced by fluoride.

グルタチオンのセレンアナログ、グルタセレノンの触媒するグルタチオンペルオキシダーゼ反応の機構査読

江崎信芳, 田村隆, 中里仁, 左右田健次

Biomed. Res. Trace Elements,4,87-88 1993年

Synthesis and characterization of the selenium analog of glutathione disulfide

Takashi Tamura, Tadao Oikawa, Akira Ohtaka, Nobutaka Fujii, Nobuyoshi Esaki, Kenji Soda

Analytical Biochemistry 208 ( 1 ) 151 - 154 1993年

記述言語：英語

We synthesized the selenium analog of glutathione disulfide by a liquid phase method and named it glutaselenone (i.e., γ-L-glutamyl-L-selenocysteinylglycine) diselenide. The selenol of selenocysteine was protected by the p-methoxybenzyl group, which was removed by acidolysis with trifluoroacetic acid in the presence of thioanisol. The overall yield of the final product, glutaselenone diselenide, was about 9% based on the starting compound, Se-(p-methoxybenzyl)-L-selenocysteine. Glutaselenone diselenide showed a broad absorption band between 270 and 400 nm and circular dichroism bands around 270 nm (positive) and 330 nm (negative), which were attributable to diselenide bond. © 1993 Academic Press, Inc.

DOI： 10.1006/abio.1993.1021