研究者詳細 - 高木　章乃夫

MISC - 高木　章乃夫

分割表示 449 件中 81 - 100 件目／全件表示 >>

肝移植1年後生存に関与する潜在的門脈肺高血圧症の重要性

高木章乃夫, 安中哲也, 麻植浩樹, 中村進一郎, 大西秀樹, 桑木健志, 中村一文, 楳田祐三, 八木孝仁, 岡田裕之

超音波医学 43 ( 6 ) 776 - 777 2016年11月

非小細胞肺癌切除症例における術前血漿中抗RPL29抗体価と予後

山本寛斉, 高木章乃夫, 林達朗, 古川公之, 田尾裕之, 枝園和彦, 宗淳一, 大谷真二, 三好健太郎, 杉本誠一郎, 山根正修, 大藤剛宏, 岡部和倫, 三好新一郎, 豊岡伸一

肺癌 56 ( 6 ) 518 - 518 2016年11月

miR-1246 and miR-4644 in salivary exosome as potential biomarkers for pancreatobiliary tract cancer

Tatsuya Machida, Takaaki Tomofuji, Takayuki Maruyama, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Hisataka Miyai, Hirofumi Mizuno, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Hiroyuki Okada, Manabu Morita

ONCOLOGY REPORTS 36 ( 4 ) 2375 - 2381 2016年10月

記述言語：英語出版者・発行元：SPANDIDOS PUBL LTD

Pancreatobiliary tract cancer is a highly fatal cancer. Detection of pancreatobiliary tract cancer is difficult because it lacks typical clinical symptoms and because of its anatomical location. Biomarker discovery is therefore important to detect pancreatobiliary tract cancer in its early stage. A study demonstrated that expression levels of miR-1246, miR-3976, miR-4306, and miR-4644 in serum exosomes were higher in pancreatic cancer patients than these levels in healthy control participants. Supposing that microRNA (miRNA) expression profiles in saliva are similar to those in serum, four miRNAs (miR-1246, miR-3976, miR-4306, and miR-4644) in salivary exosomes may also be useful for detection of pancreatobiliary tract cancer. In this study, it was examined whether these miRNAs could be used as biomarkers for pancreatobiliary tract cancer. Twelve pancreatobiliary tract cancer patients and 13 healthy control participants were analyzed as a cancer and a control group, respectively. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. Using quantitative real-time PCR (RT-qPCR), the relative expression ratios of miR-1246 and miR-4644 were significantly higher in the cancer group than these ratios in the control group. Receiver operating characteristic (ROC) curves were constructed to analyze the discrimination power of these miRNAs. For miR-1246, the results yielded an area under the curve (AUC) of 0.814 (P=0.008). For miR-4644, the results yielded an AUC of 0.763 (P=0.026). For the combination of miR-1246 and miR-4644, the results yielded an increased AUC of 0.833 (P=0.005). This pilot study suggests that miR-1246 and miR-4644 in salivary exosomes could be candidate biomarkers for pancreatobiliary tract cancer.

DOI： 10.3892/or.2016.5021

Web of Science

researchmap

肝 C型肝炎についてup to date 肝移植後のC型肝炎ウイルス治療の進歩ソホスブビル含有治療の有用性

高木章乃夫, 安中哲也, 足立卓哉, 池田房雄, 篠浦先, 楳田祐三, 吉田龍一, 信岡大輔, 高木弘誠, 八木孝仁, 岡田裕之

移植 51 ( 総会臨時 ) 223 - 223 2016年9月

肝移植後の腎機能保持を狙った降圧戦略

高木章乃夫, 安中哲也, 八木孝仁, 篠浦先, 楳田祐三, 吉田龍一, 信岡大輔, 岡田裕之

移植 51 ( 総会臨時 ) 237 - 237 2016年9月

乏血性腫瘤におけるEOB-MRIを用いた肝癌の診断手順

土肥千紘, 能祖一裕, 赤穂宗一郎, 和田望, 森元裕貴, 竹内康人, 桑木健志, 大西秀樹, 池田房雄, 中村進一郎, 白羽英則, 高木章乃夫, 岡田裕之

肝臓 57 ( Suppl.2 ) A610 - A610 2016年9月

肝動注化学療法から分子標的薬への切り替えのタイミング肝機能と早期治療効果判定の観点から

大西秀樹, 能祖一裕, 中村進一郎, 和田望, 安中幸, 竹内康人, 安中哲也, 桑木健志, 白羽英則, 高木章乃夫, 岡田裕之

肝臓 57 ( Suppl.2 ) A562 - A562 2016年9月

肝硬変に至らない原発性胆汁性肝硬変が続発性骨粗鬆症の成因となりうるか?

關杏奈, 池田房雄, 宮武宏和, 藤岡真一, 安東正晴, 高口浩一, 和田望, 森元裕貴, 安中幸, 竹内康人, 安中哲也, 桑木健志, 大西秀樹, 中村進一郎, 白羽英徳, 岩崎良章, 能祖一裕, 高木章乃夫, 岡田裕之

日本消化器病学会雑誌 113 ( 臨増大会 ) A733 - A733 2016年9月

Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer

H. Sawahara, H. Shiraha, D. Uchida, H. Kato, T. Nagahara, M. Iwamuro, J. Kataoka, S. Horiguchi, M. Watanabe, M. Sakaguchi, A. Takaki, K. Nouso, Y. Nasu, H. Kumon, H. Okada

CANCER GENE THERAPY 23 ( 8 ) 278 - 283 2016年8月

記述言語：英語出版者・発行元：NATURE PUBLISHING GROUP

Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3), a tumor suppressor gene, is downregulated in various cancers. We previously reported the tumor-inhibitory effects of the REIC/Dkk-3 gene, delivered by a conventional adenoviral vector (Ad-CAG-REIC) in pancreatic cancer. Here, we developed an Ad-REIC vector with a novel gene expression system, termed the super gene expression (SGE) system, and assessed its therapeutic effects relative to those of Ad-CAG-REIC in pantreatic cancer cells. Human pancreatic cancer cell lines ASPC1 and MIAPaCa2 were used. REIC/Dkk-3 expression was assessed by western blot analysis. Relative cell viability and apoptotic effects were examined in vitro. The anti-tumor effects of Ad-REIC treatment were assessed in the mouse xenograft model. Compared with Ad-CAG-REIC, Ad-SGE-REIC elicited a significant increase in REIC protein expression in the cells studied, Relative to Ad-CAG-REIC, Ad-SGE-REIC reduced cell viability and induced apoptosis in the ASPC1 and MIAPaCa2 cell lines in vitro, and achieved superior tumor growth inhibition in the mouse xenograft model. Compared with conventional Ad-REIC agents, Ad-SGE-REIC provided enhanced inhibitory effects against tumor growth. Our results indicate that Ad-SGE-REIC is an innovative therapeutic tool for pancreatic cancer.

DOI： 10.1038/cgt.2016.31

Web of Science

researchmap

Potential of alpha-fetoprotein as a prognostic marker after curative radiofrequency ablation of hepatocellular carcinoma

Chihiro Dohi, Kazuhiro Nouso, Koji Miyahara, Yuki Morimoto, Nozomu Wada, Hideaki Kinugasa, Yasuto Takeuchi, Kenji Kuwaki, Hideki Onishi, Fusao Ikeda, Shinichiro Nakamura, Hidenori Shiraha, Akinobu Takaki, Hiroyuki Okada

HEPATOLOGY RESEARCH 46 ( 9 ) 916 - 923 2016年8月

記述言語：英語出版者・発行元：WILEY

AimRecurrence of hepatocellular carcinoma (HCC) is observed frequently, even after curative treatments. The aim of this study is to elucidate the risk factors for recurrence of HCC after radiofrequency ablation (RFA), focusing on the carcinogenic potential of the liver assessed by -fetoprotein (AFP).
MethodsWe enrolled 357 consecutive patients who underwent complete ablation by RFA for primary HCC (3cm, 3 tumors) and analyzed the correlation between 17 critical parameters, including AFP and HCC recurrence.
ResultsRecurrence was observed in 236 patients during a mean observation period of 54.3months. Multivariate analysis revealed that multiple tumors (risk ratio [RR]=1.70, 95% confidence interval [CI]=1.27-2.26, P<0.001), high AFP (>10ng/mL, RR=1.45, 95% CI=1.09-1.94, P<0.001) and high des--carboxyprothrombin (>40 mAU/mL, RR=1.52, 95% CI=1.13-2.02, P<0.005) were significantly correlated with recurrence. AFP was selected as a significant factor even when the cut-off level was set lower (5ng/mL). The risk of recurrence increased linearly according to the increase of the lowest AFP level after RFA and the adjusted ratios relative to AFP less than 5ng/mL were 1.56, 2.14, 2.57 and 3.13 in AFP 5-10ng/mL, 10-20ng/mL, 20-50ng/mL and over 50ng/mL, respectively. In addition, the recurrence rate was predicted by the AFP level after RFA, regardless of the level before the treatment.
ConclusionAFP less than 5ng/mL after curative RFA was an important predictor of a better prognosis and was considered to indicate the low carcinogenic potential of the non-cancerous liver.

DOI： 10.1111/hepr.12636

Web of Science

researchmap

肝移植と門脈圧亢進症肝移植1年後生存に関与する術前リスクとしての潜在的門脈肺高血圧症の重要性

皿谷洋祐, 高木章乃夫, 足立卓哉, 安中哲也, 麻植浩樹, 中村一文, 楳田祐三, 八木孝仁, 岡田裕之

日本門脈圧亢進症学会雑誌 22 ( 3 ) 101 - 101 2016年8月

【「移植臓器における原疾患の再発」】移植臓器におけるC型肝炎の再発

高木章乃夫, 安中哲也, 信岡大輔, 吉田龍一, 楳田祐三, 篠浦先, 八木孝仁

移植 51 ( 2-3 ) 92 - 99 2016年8月

記述言語：日本語出版者・発行元：(一社)日本移植学会

<p>Hepatitis C recurrence after orthotopic liver transplantation (OLT) has been accepted as a major problem after OLT. Recent progress in hepatitis C virus (HCV) treatment with the use of direct acting antivirals (DAA) has almost resolved this major issue, as 95-100% of non-OLT hepatitis C patients in Japan reach a sustained viral response (SVR). However, the SVR rate among post-OLT hepatitis C patients in the United States and Europe is reported to be 90% and 10% of these patients require an additional approach. As the pathogenesis of hepatitis C involves immunological responses, post OLT hepatitis C exhibits complex phenomena with immune regulating agents including prednisolone and calcineurin inhibitors and HLA-mismatch in the recipient immune system and the donor liver. The pathogenesis and the recent progress in post-OLT hepatitis C are described in this chapter.</p>

DOI： 10.11386/jst.51.2-3_092

CiNii Article

researchmap

肝移植と門脈圧亢進症肝移植予定患者における門脈圧亢進症性小腸炎の検討前向きコホート研究の結果から

川野誠司, 高木章乃夫, 安中哲也, 楳田祐三, 八木孝仁, 岡田裕之

日本門脈圧亢進症学会雑誌 22 ( 3 ) 101 - 101 2016年8月

Synergistic anti-pancreatic cancer immunological effects by treatment with reduced expression in immortalized cells/dickkopf-3 protein and peripheral blood mononuclear cells

Daisuke Uchida, Hidenori Shiraha, Hironari Kato, Hiroaki Sawahara, Teruya Nagahara, Masaya Iwamuro, Junro Kataoka, Shigeru Horiguchi, Masami Watanabe, Akinobu Takaki, Kazuhiro Nouso, Yasutomo Nasu, Hiromi Kumon, Kazuhide Yamamoto

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 31 ( 6 ) 1154 - 1159 2016年6月

記述言語：英語出版者・発行元：WILEY-BLACKWELL

Background and AimReduced expression in immortalized cells/dickkopf-3 (REIC/DKK3) is a reported tumor suppressor gene and has potential to become an innovative therapy for various cancers. We examined the antitumor immunological effects of human REIC/DKK3 protein against pancreatic cancer.
MethodsActivation of extracellular signal-regulated kinases 1 and 2, mammalian target of rapamycin, and signal transducer and activator of transcription 3 by REIC/DKK3 protein was assessed in human peripheral blood mononuclear cells using immunoblotting. Pancreatic cancer cell lines (AsPC-1 and MIA Paca-2) were cocultured with peripheral blood mononuclear cells, and the anticancer effects of REIC/DKK3 protein were assessed using the methyl thiazole tetrazolium, cytotoxicity, and enzyme-linked immunospot assays. The antitumor immunological effects of the combined treatment with REIC/DKK3 protein and peripheral blood mononuclear cells were also assessed in a pancreatic cancer model using non-obese diabetic/severe combined immunodeficiency mice.
ResultsThe REIC/DKK3 protein activated extracellular signal-regulated kinases 1 and 2, mammalian target of rapamycin, and signal transducer and activator of transcription 3 in peripheral blood mononuclear cells. REIC/DKK3 protein inhibited in vitro cancer cell viability and enhanced cytotoxicity when incubated with peripheral blood mononuclear cells. REIC/DKK3 protein induced significant production of interferon gamma from lymphocytes incubated with pancreatic cancer cells, indicating that CD8+ T cells were activated in the peripheral blood mononuclear cells when cocultured with AsPC-1 and MIA Paca-2 in the presence of REIC/DKK3 protein. Combined treatment with REIC/DKK3 protein and peripheral blood mononuclear cells produced in vivo anticancer immunostimulatory effects on pancreatic cancer cells.
ConclusionsThe REIC/DKK3 protein and peripheral blood mononuclear cells synergistically enhanced anticancer immunological effects against pancreatic cancer cells. The observed immunomodulatory effect of combined treatment likely occurs in adenovirus-mediated REIC/DKK3 gene therapy and provides important clues to the therapeutic mechanisms involving immune cells.

DOI： 10.1111/jgh.13259

Web of Science

researchmap

Loss of Runt-related transcription factor 3 induces resistance to 5-fluorouracil and cisplatin in hepatocellular carcinoma

Junro Kataoka, Hidenori Shiraha, Shigeru Horiguchi, Hiroaki Sawahara, Daisuke Uchida, Teruya Nagahara, Masaya Iwamuro, Hiroki Morimoto, Yasuto Takeuchi, Kenji Kuwaki, Hideki Onishi, Shinichiro Nakamura, Akinobu Takaki, Kazuhiro Nouso, Takahito Yagi, Kazuhide Yamamoto, Hiroyuki Okada

ONCOLOGY REPORTS 35 ( 5 ) 2576 - 2582 2016年5月

記述言語：英語出版者・発行元：SPANDIDOS PUBL LTD

Runt-related transcription factor 3 (RUNX3) is known to function as a tumor suppressor in gastric cancer and other types of cancers, including hepatocellular carcinoma (HCC). However, its role has not been fully elucidated. In the present study, we aimed to evaluate the role of RUNX3 in HCC. We used the human HCC cell lines Hep3B, Huh7 and HLF; RUNX3 cDNA was introduced into Hep3B and Huh7 cells, which were negative for endogenous RUNX3 expression, and RUNX3 siRNA was transfected into HLF cells, which were positive for endogenous RUNX3. We analyzed the expression of RUNX3 and multidrug resistance-associated protein (MRP) by immunoblotting. MTT assays were used to determine the effects of RUNX3 expression on 5-fluorouracil (5-FU) and cisplatin (CDDP) sensitivity. Finally, 23 HCC specimens resected from patients with HCC at Okayama University Hospital were analyzed, and correlations among immunohistochemical expression of RUNX3 protein and MRP protein were evaluated in these specimens. Exogenous RUNX3 expression reduced the expression of MRP1, MRP2, MRP3 and MRP5 in the RUNX3-negative cells, whereas knockdown of RUNX3 in the HLF cells stimulated the expression of these MRPs. An inverse correlation between RUNX3 and MRP expression was observed in the HCC tissues. Importantly, loss of RUNX3 expression contributed to 5-FU and CDDP resistance by inducing MRP expression. These data have important implications in the study of chemotherapy resistance in HCC.

DOI： 10.3892/or.2016.4681

Web of Science

researchmap

Oxidative Stress Controlling Agents Are Effective for NSAIDs-Induced Small Intestinal Injuries

Yoshiyasu Kono, Akinobu Takaki, Seiji Kawano, Hiroyuki Okada

GASTROENTEROLOGY 150 ( 4 ) S693 - S693 2016年4月

Characteristics of HCC Patients With hTERT Promoter Mutation

Soichiro Ako, Kazuhiro Nouso, Hideaki Kinugasa, Takuya Adachi, Chihiro Dohi, Yuuki Morimoto, Nozomu Wada, Yasuto Takeuchi, Kenji Kuwaki, Hideki Onishi, Fusao Ikeda, Shinichiro Nakamura, Hidenori Shiraha, Akinobu Takaki, Shinichi Fujioka, Hiroyuki Okada

GASTROENTEROLOGY 150 ( 4 ) S1152 - S1152 2016年4月

The Pattern of Recurrence Associated With the Prognosis of the Patients With Hepatocellular Carcinoma After Radiofrequency Ablation

Shinichiro Nakamura, Kazuhiro Nouso, Hideki Onishi, Kenji Kuwaki, Yasuto Takeuchi, Nozomu Wada, Yuuki Morimoto, Tetsuya Yasunaka, Yuki Yasunaka, Fusao Ikeda, Hidenori Shiraha, Akinobu Takaki, Kazuhide Yamamoto, Hiroyuki Okada

GASTROENTEROLOGY 150 ( 4 ) S1135 - S1135 2016年4月

Serum IP-10 Is a Highly Sensitive Marker for Discriminating Non-Alcoholic Fatty Liver and Non-Alcoholic Steatohepatitis

Nozomu Wada, Akinobu Takaki, Fusao Ikeda, Tetsuya Yasunaka, Masahiro Onji, Kazuko Koike, Kazuhiro Nouso, Hiroyuki Okada

GASTROENTEROLOGY 150 ( 4 ) S1091 - S1091 2016年4月

データマイニング手法を用いた非侵襲的肝線維化診断法によるC型肝硬変症例の抽出

桑木健志, 中村進一郎, 安中幸, 竹内康人, 安中哲也, 大西秀樹, 池田房雄, 白羽英則, 高木章乃夫, 岡田裕之

超音波医学 43 ( Suppl. ) S673 - S673 2016年4月

　前のページ - 次のページ　

<高木 章乃夫>

MISC - 高木 章乃夫

<高木　章乃夫>

MISC - 高木　章乃夫