研究者詳細 - 久保田　聡

論文 - 久保田　聡

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制御遺伝子をターゲットとした抗HIV戦略招待

久保田聡

Molecular Medicine 30: 773 - 775 1993年

Inhibition of human immunodeficiency virus type 1 rev function by a rev mutant which interferes with nuclear / nucleolar localization of rev.（共著）査読

Kubota, S, R. Furuta, M. Maki, M. Hatanaka

Journal of Virology 66 ( 4 ) 2510 - 2513 1992年4月

FUNCTIONAL CONVERSION FROM HIV-1 REV TO HTLV-1 REX BY MUTATION 査読

S KUBOTA, T NOSAKA, R FURUTA, M MAKI, M HATANAKA

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 178 ( 3 ) 1226 - 1232 1991年8月

EFFECTS OF CHIMERIC MUTANTS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV AND HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I REX ON NUCLEOLAR TARGETING SIGNALS 査読

S KUBOTA, T NOSAKA, BR CULLEN, M MAKI, M HATANAKA

JOURNAL OF VIROLOGY 65 ( 5 ) 2452 - 2456 1991年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC MICROBIOLOGY

Two chimeric mutant genes derived from rev of human immunodeficiency virus type 1 and rex of human T-cell leukemia virus type I were constructed to investigate the functions of the nucleolar-targeting signals (NOS) in Rev and Rex proteins. A chimeric Rex protein whose NOS region was substituted with the NOS of Rev was located predominantly in the cell nucleolus and functioned like the wild-type protein in the Rex assay system. However, a chimeric Rev with the NOS of Rex abolished Rev function despite its nucleolar localization. This nonfunctional nucleolar-targeting chimeric protein inhibited the function of both Rex and Rev. In the same experimental conditions, this mutant interfered with the localization of the functional Rex in the nucleolus.

Web of Science

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2,3 DIMERCAPTO-1-PROPANOL INHIBITS HIV-1 TAT ACTIVITY, VIRAL PRODUCTION, AND INFECTIVITY INVITRO 査読

S KUBOTA, MA ELFARRASH, M MAKI, S HARADA, M HATANAKA

AIDS RESEARCH AND HUMAN RETROVIRUSES 6 ( 7 ) 919 - 927 1990年7月

Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA

T. Nosaka, H. Siomi, Y. Adachi, M. Ishibashi, S. Kubota, M. Maki, M. Hatanaka

Proceedings of the National Academy of Sciences of the United States of America 86 ( 24 ) 9798 - 9802 1989年12月

The posttranscriptional regulator (rex) of human T-cell leukemia virus type I is known to be located predominantly in the cell nucleolus and to induce the accumulation of gag and env viral mRNAs. The N-terminal 19 amino acids of rex-encoded protein (Rex) has been shown to be sufficient to direct hybrid proteins to the cell nucleolus. We have studied the function of the nucleolar targeting signal (NOS) of rex by using full-length proviral DNA and mutant rex expression plasmids. Partial deletions of the NOS sequence abolished the accumulation of unspliced cytoplasmic mRNA, although the gene products of rex mutants were found in the nucleoplasm. These results indicate that NOS sequence, or nucleolar localization of Rex, is essential for Rex function.

DOI： 10.1073/pnas.86.24.9798

A region of basic amino-acid cluster in HIV-1 Tat protein is essential for trans-acting activity and nucleolar localization. 査読国際誌

S Endo, S Kubota, H Siomi, A Adachi, S Oroszlan, M Maki, M Hatanaka

Virus genes 3 ( 2 ) 99 - 110 1989年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

The trans-acting factor of human immunodeficiency virus (HIV), Tat, has a basic amino-acid cluster that is highly conserved among different HIV isolates. We have examined the effects of mutations in the basic region of Tat on its trans-acting activity and cellular localization. Introduction of a stop codon immediately preceding the basic region abolished the activity, while the truncated mutant with the basic region retained some activity. The basic region of Tat was replaceable with that of Rev (another trans-acting factor of HIV) but not with that of adenovirus Ela nor cellular enzyme. The result of immunofluorescence analysis revealed a correlation between the nuclear, especially nucleolar, accumulation and the activities of mutant Tat proteins.

PubMed

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FUNCTIONAL SIMILARITY OF HIV-I REV AND HTLV-I REX PROTEINS - IDENTIFICATION OF A NEW NUCLEOLAR-TARGETING SIGNAL IN REV PROTEIN 査読

S KUBOTA, H SIOMI, T SATOH, S ENDO, M MAKI, M HATANAKA

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 162 ( 3 ) 963 - 970 1989年8月

Role of the cysteine-rich region of HIV tat protein on its transactivational ability.（共著）査読

Kubota, S, S. Endoh, M. Maki, M. Hatanaka

Virus Genes 2 113 - 118 1988年

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