研究者詳細 - 久保田　聡

論文 - 久保田　聡

分割表示 229 件中 201 - 220 件目／全件表示 >>

Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells

S Kubota, T Hattori, T Shimo, T Nakanishi, M Takigawa

FEBS LETTERS 474 ( 1 ) 58 - 62 2000年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. Tn Cos-7 cells, expression of human CTGF induced an altered cell morphology, It was associated with an increased cellular DNA content and loose attachment, indicating the cells mere in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly, These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery. (C) 2000 Federation of European Biochemical Societies.

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Role of lentiviral lytic polypeptide I (LLP-I) in the selective cytotoxicity of gamma-glutamylcysteine ethyl ester against human immunodeficiency virus type 1-producing cells

S Kubota, H Zhang, S Kitahara, RJ Pomerantz

ANTIVIRAL CHEMISTRY & CHEMOTHERAPY 10 ( 3 ) 121 - 127 1999年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：INT MEDICAL PRESS

Selective cytotoxic effects of gamma-glutamylcysteine ethyl ester (gamma-CCE) against human immunodeficiency virus type 1 (HIV-l)-infected H-9 T lymphocytic cells were demonstrated previously. However, the mechanism of those effects remained unclear. Here, we report on enhanced cytotoxicity of the lentiviral lytic peptide I (LLP-1) of gp41, the envelope transmembrane glycoprotein of HIV-I. in the presence of gamma-GCE. Without gamma-GCE, the cytotoxic effect of LLP-1 was transient, whereas with gamma-GCE, cell death induced by LLP-1 remained continuous until termination. Of note, such effects by gamma-GCE were also observed with another unrelated amphipathic peptide toxin, melittin. These results suggest that the synergistic cytotoxic effect of gamma-GCE and LLP-1 may play a central role in the molecular mechanism of the selective cytotoxicity of gamma-GCE in HIV-l-infected T lymphocytic cells.

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Involvement of cis-acting repressive element(s) in the 3ﾕ-untranslated region of human connective tissue growth factor gene

Kubota, S, Hattori, T, Nakanishi, T, Takigawa, M

FEBS Letters 450 ( 1-2 ) 84 - 88 1999年4月

Nuclear and nucleolar targeting of human ribosomal protein S25: Common features shared with HIV-1 regulatory proteins

S Kubota, TD Copeland, RJ Pomerantz

ONCOGENE 18 ( 7 ) 1503 - 1514 1999年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：STOCKTON PRESS

The nuclear and nucleolar targeting properties of human ribosomal protein S25 (RPS25) were analysed by the expression of epitope-tagged RPS25 cDNAs in Cos-l cells. The tagged RPS25 was localized to the cell nucleus, with a strong predominance in the nucleolus. At the amino terminus of RPS25, two stretches of highly basic residues juxtapose. This configuration shares common features with the nucleolar targeting signals (NOS) of lentiviral RNA-binding transactivators, including human immunodeficiency viruses' (HIV) Rev proteins. Deletion and site-directed mutational analyses demonstrated that the first NOS-like stretch is dispensable for both nuclear and nucleolar localization of RPS25, and that the nuclear targeting signal is located within the second NOS-like stretch. It has also been suggested that a set of continuous basic residues and the total number of basic residues should be required for nucleolar targeting. Signal-mediated nuclear/nucleolar targeting was further characterized by the construction and expression of a variety of chimeric constructs, utilizing three different backbones with RPS25 cDNA fragments. Immunofluorescence analyses demonstrated a 17 residue peptide of RPS25 as a potential nuclear/nucleolar targeting signal. The identified peptide signal may belong to a putative subclass of NOS, characterized by compact structure, together with lentiviral RNA binding transactivators.

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HIV mRNAの核外輸送とRev - スプライシングを免れて核外にでる方法

久保田聡

医学のあゆみ 189: 1009-1010 1999年

Novel inhibitory effects of g-glutamylcysteine ethyl ester against human immunodeficiency virus type 1 production and propagation.（共著）

Kubota, S, S. Shetty, H. Zhang, S. Kitahara, R.J. Pomerantz

Antimicrobial Agents and Chemotherapy 42 ( 5 ) 1200 - 1206 1998年5月

A cis-acting peptide signal in human immunodeficiency virus type-1 Rev which inhibits nuclear entry of small proteins.（共著）査読

Kubota, S, R.J. Pomerantz

Oncogene 16 ( 14 ) 1851 - 1861 1998年4月

Modulation of HTLVL-I gene expression by HIV-1 Rev through an alternative RxRE-independent pahtway mediated by the RU5 portion of the 5’-LTR.（共著）査読

Kubota, S, Furuta, R.A, M. Hatanaka, R.J. Pomerantz

Biochemical and Biophysical Research Communications 243 ( 1 ) 79 - 85 1998年2月

Cis/trans-activation of the interleukin-9 receptor gene in an HTLV-I -transformed human lymphocytic cell.（共著）査読

Kubota, S, R.J. Pomerantz

Oncogene 12 1441 - 1447 1998年

Nucleo-cytoplasmic re-distribution of the HTLV-I Rex protein: Alterations by co-expression of the HTLV-I p21x protein.（共著）査読

Kubota, S, M. Hatanaka, R.J. Pomerantz

Virology 220 ( 2 ) 502 - 507 1996年6月

Binding of intracellular anti-Rev SFvs to different epitopes of HIV-1 Rev: Variations in viral inhibition.（共著）査読

Wu, Y, L. Duan, M. Zhu, S. Kubota, O. Bagasra, R.J. Pomerantz

Journal of Virology 70 ( 5 ) 3290 - 3297 1996年5月

Nuclear preservation and cytoplasmic degradation of human immunodeficiency virus type 1 Rev protein.（共著）査読

Kubota, S, L. Duan, R.A. Furuta, M. Hatanaka, R.J. Pomerantz

Journal of Virology 70 ( 2 ) 1282 - 1287 1996年2月

Analysis of a novel defective HTLV-I provirus and detection of a new HTLV-I -induced cellular transcript.（共著）査読

Kubota, S, R.A. Furuta, H. Siomi, M. Maki, M. Hatanaka

FEBS Letters 375 ( 1-2 ) 31 - 36 1995年11月

Binding of human prothymosin a to the leucine-motif/activation domains of HTLV-I Rex and HIV-1 Rev.（共著）査読

Kubota, S, Y. Adachi, T.D. Copeland, S. Oroszlan

European Journal of Biochemistry 233 ( 1 ) 48 - 54 1995年10月

Use of a human immunodeficiency virus type 1 Rev mutant without nucleolar dysfunction as a candidate for potential AIDS therapy.（共著）査読

Furuta,R.A, S. Kubota, M. Maki, Y. Miyazaki, T. Hattori, M Hatanaka

Journal of Virology 69 ( 3 ) 1591 - 1599 1995年3月

The origin of HIV-I rev gene : an evolutionary hypothesis.（jointly worked）査読

Kubota, S, S. Oroszlan, M. Hatanaka

FEBS Letters 338 ( 2 ) 118 - 121 1994年1月

ウィルスと宿主の関係性の進化査読

畑中正一, 久保田聡

蛋白質核酸酵素 39 2457 - 2464 1994年

Cytotoxic activity of rev protein of human immunodeficiency virus type 1 by nucleolar dysfunction.（共著）査読

Nosaka, T, T. Takamatsu, Y. Miyazaki, K. Sano, A. Sato, S. Kubota, M. Sakurai, Y. Ariumi, M. Nakai, S. Fujita, M. Hatanaka

Experimental Cell Research 209 ( 1 ) 89 - 102 1993年11月

Long cellular repeats flanking a defective HTLV-I provirus: implication for site-targeted integration.（共著）査読

Kubota, S, R. Furuta, M. Maki, H. Siomi, M. Hatanaka

Oncogene 8 ( 10 ) 2873 - 2877 1993年10月

HIVとRadical Scavenger 招待

久保田聡

メディカルイムノロジー 26 453 - 459 1993年

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