研究者詳細 - 江國　大輔

MISC - 江國　大輔

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Beating stimulation promotes proliferative activity in rat gingival cells.

Ekuni D, Yamamoto T, Yamanaka R, Tomofuji T, Watanabe T

Dentistry in Japan 2005年

ラット歯周炎モデルに対するグリチルリチン酸ジカリウム、クレイツイオンを配合した歯磨剤と機械的刺激との効果．

山本龍生, 江國大輔, 山中玲子, 坂本友紀, 林浩範, 渡邊達夫

口腔衛生学会雑誌 55 ( 5 ) 537 - 542 2005年

記述言語：日本語出版者・発行元：有限責任中間法人日本口腔衛生学会

ラットの歯周炎に対する, グリチルリチン酸ジカリウム, クレイツイオン^[○!R]ハイパワー, クレイツイオン^[○!R]ナンバーワンを配合した歯磨剤の効果, および歯磨剤と機械的刺激を併用したときの効果を検討した.ラット40匹の両側上顎第一臼歯口蓋側歯肉溝に細菌由来のリポポリサッカライドとプロテアーゼを4週間塗布し, 歯周炎を惹起した.その後, それらに加えて4種類の歯磨剤(対照歯磨剤, グリチルリチン酸ジカリウム配合歯磨剤, グリチルリチン酸ジカリウムおよびクレイツイオン^[○!R]ハイパワー配合歯磨剤, グリチルリチン酸ジカリウムおよびクレイツイオン^[○!R]ナンバーワン配合歯磨剤)を4週間塗布し, 対照歯磨剤群以外のラットの左側第一臼歯口蓋側歯肉を電動歯ブラシで1日1回機械的に刺激した.屠殺後, 歯肉のヘマトキシリン・エオジン染色標本を作製し, 組織学的に比較検討した.その結果, グリチルリチン酸ジカリウムにクレイツイオン^[○!R]ハイパワーまたはクレイツイオン^[○!R]ナンバーワンを配合した歯磨剤は, それぞれ結合組織中または接合上皮内の好中球を有意に減少させた.また, グリチルリチン酸ジカリウム配合歯磨剤塗布に歯肉への機械的刺激を加えると, グリチルリチン酸ジカリウムにクレイツイオン^[○!R]ハイパワーまたはクレイツイオン^[○!R]ナンバーワンを配合した歯磨剤と同程度の結合組織中または接合上皮内の好中球を減少させる効果が得られた.

DOI： 10.5834/jdh.55.5_537

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その他リンク： http://search.jamas.or.jp/link/ui/2006032082

Gingival cell proliferation induced by use of a sonic toothbrush with warmed silicone rubber bristles 国際誌

T Tomofuji, H Kusano, T Azuma, D Ekuni, T Yamamoto, T Watanabe, T Kishimoto

JOURNAL OF PERIODONTOLOGY 75 ( 12 ) 1636 - 1639 2004年12月

記述言語：英語出版者・発行元：AMER ACAD PERIODONTOLOGY

Background: Toothbrushing promotes gingival cell proliferation, which may occur as the result of the physical stimulation of the gingiva. The present study evaluated the effects of temperature and silicone rubber bristles of a sonic toothbrush on gingival cell proliferation in dogs.
Methods: During the 5-week experimental period, one quadrant in each of eight dogs received a different toothbrushing regimen: a manual toothbrush or a sonic toothbrush with 1) nylon, 2) silicone rubber, or 3) warmed silicone rubber bristles. The proliferative activity of gingival cells was evaluated based on expression of proliferating cell nuclear antigen (PCNA).
Results: Use of the sonic toothbrushes produced a higher density of PCNA-positive and total fibroblasts than did use of a manual toothbrush. The warm silicone rubber bristles resulted in a higher density of PCNA-positive fibroblasts compared with the cooler silicone rubber bristle. The number of PCNA-positive basal cells in the junctional epithelium also increased following electric toothbrushing with warmed silicone rubber bristles.
Conclusions: The sonic toothbrush with silicone rubber bristles induced gingival fibroblast proliferation to a greater degree than a manual toothbrush. Warming the silicone rubber bristles increased their stimulatory effects on the proliferative activity of gingival cells.

DOI： 10.1902/jop.2004.75.12.1636

草野弘揮, 東哲司, 友藤孝明, 江國大輔, 坂本友紀, 山本龍生, 渡邊達夫, 岸本隆

口腔衛生学会雑誌 54 ( 5 ) 586 - 586 2004年10月

歯肉線維芽細胞の増殖能とコラーゲン合成に及ぼす超音波と振動の影響

東哲司, 草野弘揮, 江國大輔, 坂本友紀, 友藤孝明, 山本龍生, 渡邊達夫

日本歯周病学会会誌 46 ( 秋季特別 ) 140 - 140 2004年9月

ラット歯周病モデルにおける高コレステロール食の影響

友藤孝明, 東哲司, 草野弘揮, 江國大輔, 山本龍生, 渡邊達夫

日本歯周病学会会誌 46 ( 秋季特別 ) 111 - 111 2004年9月

ラット歯周病モデルにおいて機械的刺激は歯周炎の治癒を促進する

山中玲子, 江國大輔, 山本龍生, 渡邊達夫

口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH 54 ( 4 ) 324 - 324 2004年8月

ラット歯周病モデルにおける機械的刺激の影響

山本龍生, 江國大輔, 山中玲子, 渡邊達夫

日本歯周病学会会誌 46 ( 春季特別 ) 123 - 123 2004年4月

機械的刺激によるラット歯肉の接合上皮基底細胞と線維芽細胞の細胞周期の変化

山中玲子, 江國大輔, 草野弘輝, 山本龍生, 渡邊達夫

日本歯周病学会会誌 46 ( 春季特別 ) 157 - 157 2004年4月

Effects of toothbrushing frequency on proliferation of gingival cells and collagen synthesis 国際誌

T Yamamoto, T Tomofuji, D Ekuni, T Sakamoto, M Horiuchi, T Watanabe

JOURNAL OF CLINICAL PERIODONTOLOGY 31 ( 1 ) 40 - 44 2004年1月

記述言語：英語出版者・発行元：WILEY-BLACKWELL

Background: Mechanical stimulation by toothbrushing enhances proliferation of fibroblasts and junctional epithelium (JE). These changes in gingiva may depend on the interval between toothbrushing. The effects of toothbrushing frequency on proliferation of gingival fibroblasts and basal cells of JE were evaluated.
Methods: Twelve mongrel dogs were used. Each tooth was brushed for 20 s at 1.96 N. The subepithelial connective tissue of JE was examined for proliferating cell nuclear antigen (PCNA)-positive fibroblasts and procollagen type-I C-peptide (PIP)-positive fibroblasts. JE was examined for PCNA-positive basal cells.
Results: Gingiva that received brushing twice a day showed increases in the density of fibroblasts and ratio of PCNA-positive fibroblasts to total fibroblasts at 4 weeks. The ratio of PIP-positive fibroblasts increased at 8 weeks in gingiva brushed twice a day and once a day. PCNA-positive basal cell ratio increased at 4 weeks in gingiva brushed twice a day and once a day.
Conclusions: A high frequency of brushing was associated with increased numbers of PCNA-positive fibroblasts, PIP-positive fibroblasts and PCNA-positive basal cells. Gingival cell proliferation increased and reached a plateau earlier in gingiva brushed twice a day than in gingiva brushed once a day.

DOI： 10.1111/j.0303-6979.2004.00439.x

Gingival cell responses to sonic or oscillanting/rotating electric toothbrushes.

Tomofuji T, Yamamoto T, Sakamoto T, Ekuni D, Watanabe T

Int J oral Health 2004年

Proteases augment the effects of lipopolysaccharide in rat gingiva

D Ekuni, T Yamamoto, R Yamanaka, K Tachibana, T Watanabe

JOURNAL OF PERIODONTAL RESEARCH 38 ( 6 ) 591 - 596 2003年12月

記述言語：英語出版者・発行元：BLACKWELL MUNKSGAARD

Objectives: Lipopolysaccharide (LPS) and proteases have been implicated as important factors in the initiation and progression of human periodontal diseases. A single application of LPS or proteases is insufficient to induce periodontal pocket formation or periodontitis. The aim of the present study was to assess the combined effect of lipopolysaccharide and proteases on rat periodontal tissues, and create a periodontal disease model.
Materials and methods: Forty male Wistar rats were divided into four groups: combination group (treated with both LPS and proteases solutions); LPS group; proteases group; and control. Each solution was introduced daily into the palatal gingival sulcus of maxillary molars for 8 weeks. The tissues were evaluated histometrically and immunohistochemically.
Results: In the LPS group, elongation of rete ridge, apical migration of junctional epithelium (JE), increased numbers of B cells in connective tissue, and resorption of alveolar bone were observed. In the proteases group, the increase in the number of infiltrating polymorphonuclear leukocytes and blood vessels in the connective tissue was greater than that of the LPS group.
Conclusions: The effects of LPS on periodontal tissues differed from those of proteases. The addition of proteases augmented and increased the effects of LPS, which were apical migration, intraepithelial cleavage of JE, and increased B cell density. The lesions in the combination group resembled established lesions of human periodontitis, with the exception of the low density of plasma cells.

DOI： 10.1034/j.1600-0765.2003.00694.x

Gingival cell proliferation by electric toothbrushes with various brush-heads.

T Azuma, H Kusano, T Tomofuji, D Ekuni, T Sakamoto, T Yamamoto, T Watanabe, T Kishimoto

JOURNAL OF DENTAL RESEARCH 82 423 - 423 2003年12月

ツインペッカー^【○!R】による歯肉出血の改善

下野順子, 江國大輔, 山本龍生, 渡邊達夫

日本歯周病学会会誌 45 ( 秋季特別 ) 163 - 163 2003年9月

電動歯ブラシの植毛と歯周組織の応答

草野弘揮, 東哲司, 友藤孝明, 江國大輔, 坂本友紀, 山本龍生, 渡邊達夫, 岸本隆

日本歯周病学会会誌 45 ( 秋季特別 ) 162 - 162 2003年9月

LPS, プロテアーゼによる歯周ポケット形成時の接合上皮の増殖能亢進とコラーゲン破壊

江國大輔, 山中玲子, 橘健太郎, 友藤孝明, 山本龍生, 渡邊達夫

口腔衛生学会雑誌 53 ( 4 ) 359 - 359 2003年8月

LPS and proteases induce apoptosis in periodontal ligament fibroblasts, but not in cementoblasts.

D. Ekuni, R. Yamanaka, K. Tachibana, T. Yamamoto, T. Watanabe

JOURNAL OF DENTAL RESEARCH 82 B291 - B291 2003年6月

Proliferative activity of epithelial cells and fibroblasts by mechanical stimulation.

R. Yamanaka, D. Ekuni, T. Tomofuji, T. Yamamoto, T. Watanabe

JOURNAL OF DENTAL RESEARCH 82 B356 - B356 2003年6月

Spatial extent of gingival cell activation due to mechanical stimulation by toothbrushing 国際誌

T Sakamoto, M Horiuchi, T Tomofuji, D Ekuni, T Yamamoto, T Watanabe

JOURNAL OF PERIODONTOLOGY 74 ( 5 ) 585 - 589 2003年5月

記述言語：英語出版者・発行元：AMER ACAD PERIODONTOLOGY

Background: Mechanical stimulation by toothbrushing enhances gingival fibroblast proliferation and collagen synthesis, and reduces inflammatory cell infiltration. The aim of this study was to investigate the spatial extent of proliferation of fibroblasts and endothelial cells in dog gingiva in response to mechanical stimulation by toothbrushing.
Methods: All maxillary fourth premolars and mandibular first molars of 6 mongrel dogs were used. Dental plaque was removed with a curet. One of each pair of bilateral teeth (in the same jaw) was assigned to the brushing group, and the corresponding tooth (opposite side) was assigned to the control group. The Bass method was used to brush the limited mesial half of the tooth at 1.96 N for 20 seconds with a fitted plastic stent. Immediately before fixation of tissue, the surface of brushed gingiva was notched to indicate the borderline between the brushed and non-brushed areas. Histometrical analyses of the sections were performed using assays for proliferating cell nuclear antigen (PCNA) and von Willebrand factor.
Results: The numbers of fibroblasts and PCNA-positive fibroblasts in the subepithelial connective tissue adjacent to oral sulcular epithelium significantly increased in brushed gingiva, not only in the brushed area but also in the non-brushed area 0 to 0.5 mm from the notch. Increased numbers of vascular endothelial cells were observed only in the brushed area.
Conclusion: The effect of mechanical stimulation by toothbrushing on gingival cell proliferation was not observed more than 0.5 mm from the brushed area. These results indicate that effective activation of gingival cell proliferation requires mechanical stimulation of gingiva in all areas.

DOI： 10.1902/jop.2003.74.5.585

Optimum force and duration of toothbrushing to enhance gingival fibroblast proliferation and procollagen type I synthesis in dogs 国際誌

T Tomofuji, D Ekuni, T Yamamoto, M Horiuchi, T Sakamoto, T Watanabe

JOURNAL OF PERIODONTOLOGY 74 ( 5 ) 630 - 634 2003年5月

記述言語：英語出版者・発行元：AMER ACAD PERIODONTOLOGY

Background: Toothbrushing enhances gingival fibroblast proliferation, which promotes wound healing. Optimum force and duration of toothbrushing for stimulation of fibroblast proliferation are key factors in maximizing effects of toothbrushing on periodontal wound healing. We therefore evaluated the effects of different durations and forces of toothbrushing on proliferative activity and procollagen synthesis of gingival fibroblasts.
Methods: Twelve dogs were used. In each dog, buccal gingivae of 12 teeth were examined for 3 weeks. Nine of these 12 teeth were each assigned to 1 of 9 different combinations of brushing force (0.98, 1.96, or 2.45 N) and duration (10, 20, or 40 seconds). The remaining 3 teeth received plaque removal without brushing, via a scaler.
Results: Force and duration of toothbrushing affected both proliferating cell nuclear antigen (PCNA)-positive and procollagen Type I C-peptide (PIP)-positive fibroblast ratios (P<0.05). The highest ratio of PCNA-positive fibroblasts was produced by brushing at 1.96 N for 20 seconds. The highest ratio of PIP-positive fibroblasts was produced by brushing at 1.96 N for 10 seconds.
Conclusions: Toothbrushing at certain forces and durations enhanced the proliferative activity and procollagen synthesis of gingival fibroblasts. The toothbrushing duration that increased procollagen synthesis (10 seconds) was shorter than that which increased fibroblast proliferative activity (20 seconds).

DOI： 10.1902/jop.2003.74.5.630

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MISC - 江國 大輔

<江國　大輔>

MISC - 江國　大輔