研究者詳細 - 江國　大輔

MISC - 江國　大輔

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Supplementation of broccoli or Bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet 国際誌

Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Tatsuo Yamamoto, Atsushi Ishikado, Takehiko Sato, Kayo Harada, Hirohisa Suido, Manabu Morita

NUTRITION RESEARCH 32 ( 4 ) 301 - 307 2012年4月

記述言語：英語出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

High-cholesterol diet enhances osteoclastic activity on alveolar bone by increasing serum lipid peroxidation. We hypothesized that supplementation with dietary antioxidants, such as found in broccoli and its fermented products, might suppress increases in serum lipid peroxidation, contributing to the inhibition of osteoclastic activity after high-cholesterol diet intake. The purpose of the present study was to investigate the effects of broccoli and fermented broccoli consumption on serum lipid peroxidation and osteoclast differentiation in alveolar bone of rats fed a high-cholesterol diet. In this 12-week study, rats were divided into 4 groups (n = 6/group): a control group (fed regular diet) and 3 experimental groups (fed a high-cholesterol [1% wt/wt] diet, or a high-cholesterol diet supplemented with either broccoli powder [5% wt/wt] or Bifidobacterium longum-fermented broccoli powder [5% wt/wt]). Serum hexanoyl-lysine (HEL) levels were measured as a parameter of lipid peroxidation. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in alveolar bone was enumerated to evaluate osteoclast differentiation. When compared with regular diet, the high-cholesterol diet increased serum HEL levels and resulted in a higher number of TRAP-positive osteoclasts at 12 weeks. The high-cholesterol diet supplemented with broccoli or B. longum-fermented broccoli showed lower levels of serum HEL and fewer TRAP-positive osteoclasts than the high-cholesterol diet at 12 weeks. In conclusion, consumption of broccoli, or its fermented product, inhibited the effects of a high-cholesterol diet on osteoclast differentiation in rat alveolar bone by suppressing serum lipid peroxidation. (C) 2012 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.nutres.2012.03.006

Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats 国際誌

Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Ken Yaegaki, Manabu Morita

JOURNAL OF PERIODONTOLOGY 83 ( 4 ) 522 - 527 2012年4月

記述言語：英語出版者・発行元：AMER ACAD PERIODONTOLOGY

Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll-like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study is to examine the combined effects of sodium hydrogen sulfide (NaHS, an H2S donor drug) and LPS on osteoclast differentiation and TLR expression in rat periodontal tissue.
Methods: Twenty-eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate-resistant acid phosphate (TRAP)-positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
Results: The number of TRAP-positive osteoclasts was significantly higher in the combination group than in any other group (P < 0.01). The combination group had 11.0-fold higher TLR4 mRNA levels than the control group. TLR4 protein levels were also higher in the combination group than in the NaHS or LPS group. However, the TLR2 mRNA and protein levels were not significantly different in the combination group and the LPS group.
Conclusion: In rat periodontal tissue, NaHS and LPS had an additive effect on osteoclast differentiation through activation of the TLR4 pathway but not the TLR2 pathway. J Periodontol 2012;83:522-527.

DOI： 10.1902/jop.2011.110315

Loss of claudin-1 in lipopolysaccharide-treated periodontal epithelium

T. Fujita, J. D. Firth, M. Kittaka, D. Ekuni, H. Kurihara, E. E. Putnins

JOURNAL OF PERIODONTAL RESEARCH 47 ( 2 ) 222 - 227 2012年4月

記述言語：英語出版者・発行元：WILEY-BLACKWELL

Background and Objective: The epithelial barrier is a critical component of innate immunity and provides protection against microbial invasion. Claudin-1, a tight junction protein, is known to contribute to the epithelial cell barrier. An experimentally induced rat periodontal disease model was used to study the effects of lipopolysaccharide (LPS) on the expression of tight junction-associated molecule genes in the junctional epithelium.
Material and Methods: LPS was applied for 8 wk in the gingival sulcus, and junctional epithelium was collected by laser-capture microdissection and subjected to microarray analysis.
Results: Microarray analysis identified that expression of the claudin-1 gene was decreased in the epithelium by chronic LPS challenge. Immunohistochemical analysis confirmed the expression of claudin-1 protein in junctional epithelium and that 8 wk of chronic LPS topical application significantly reduced claudin-1 expression. The effect of LPS on claudin-1 protein expression was validated using a porcine junctional epithelial cell culture Transwell model. The epithelial barrier, as measured using transmembrane resistance, was significantly reduced after 3 wk of LPS challenge and this was associated with a decreased level of expression of claudin-1 protein.
Conclusion: These results confirm that the initiation of experimental periodontal disease is associated with reduction in the expression of claudin-1 gene and protein. This decreased level of a critical tight junction protein may result in the disruption of barrier function and may play an important role in the initiation of periodontal disease.

DOI： 10.1111/j.1600-0765.2011.01424.x

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コーヒー摂取と歯周病の関連

東哲司, 友藤孝明, 江國大輔, 入江浩一郎, 遠藤康正, 粕山健太, 森田学

口腔衛生学会雑誌 62 ( 2 ) 224 - 224 2012年4月

咬合異常はラット海馬におけるβアミロイド量を増加させる

江國大輔, 友藤孝明, 入江浩一郎, 東哲司, 遠藤康正, 粕山健太, 森田学

口腔衛生学会雑誌 62 ( 3 ) 340 - 340 2012年4月

歯周病メインテナンス期患者における歯周病の進行と全身の酸化ストレスとの関連

玉木直文, 友藤孝明, 江國大輔, 森田学

日本歯周病学会会誌 54 ( 春季特別 ) 135 - 135 2012年4月

歯肉コラーゲンの加齢変化に対するピクノジェノール配合歯磨剤の効果

粕山健太, 友藤孝明, 江國大輔, 東哲司, 入江浩一郎, 遠藤康正, 森田学

日本歯周病学会会誌 54 ( 春季特別 ) 132 - 132 2012年4月

動物モデルを用いた基礎研究からヘルスプロモーションへ歯周病の研究を行ってきた一人として

江國大輔

口腔衛生学会雑誌 62 ( 2 ) 144 - 145 2012年4月

大学新入生の口腔健康状態の調査（性差および経年的変化の検討）．

児島梓, 江國大輔, 古田美智子, 入江浩一郎, 東哲司, 水谷慎介, 山中玲子, 友藤孝明, 森田学, 山本龍生, 村上隆, 山城隆, 古賀光, 岩崎良章, 清水幸登, 大西勝, 小倉俊郎

CAMPUS HEALTH 49 ( 3 ) 91 - 96 2012年

記述言語：日本語出版者・発行元：(公社)全国大学保健管理協会

岡山大学の新入生を対象とした歯科健診の結果について、性差および経年的変化の検討を行った概要を報告する。2008年度から2011年度の新入生のうち18〜19歳の者を抽出し、総計5,097名(男性2,913名、女性2,184名)を対象に口腔健康状態を分析した。う蝕の保有者は全体の7.4%〜16.1%で、性差および経年的な傾向はなかった。一方、4mm以上の歯周ポケットを有する者の割合はCPIでは11.0%〜22.6%であり、経年的に軽症化にあった。男女比較では、プロービング時出血の割合、歯垢指数、歯石指数のすべてにおいて女性が有意に少なかった。さらに、智歯が1本も萠出していない者の割合は68.6%〜72.1%で、有意に女性が多かったが、年度間の差はなかった。不正咬合の割合は27.5%〜38.9%で経年的に減少し、歯科矯正治療経験者の割合は9.1%〜12.2%で経年的に増加していた。また、男女比較では両割合ともに女性が有意に多かった。以上より、大学新入生において、女性は男性に比べて歯周状態および口腔衛生状態が優れている一方で、不正咬合を有する者が多かった。また、経年的にはう蝕の保有者の割合には一定の傾向はなかったが、歯周状態と不正咬合は改善傾向にあった。今後も大学生の口腔の健康を保持・増進するために、歯科健診や保健指導を行う必要がある。(著者抄録)

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岡山大学新入生におけるう蝕経験の有無と食生活に対する自己効力感との関係．

水谷慎介, 江國大輔, 古田美智子, 友藤孝明, 入江浩一郎, 東哲司, 児島梓, 長瀬純, 森田学

口腔衛生学会雑誌 62 ( 4 ) 403 - 409 2012年

記述言語：日本語出版者・発行元：有限責任中間法人日本口腔衛生学会

これまでう蝕のリスク因子について多くの研究がなされてきたが,食生活に対する自己効力感が関係するかどうかは不明である.本研究の目的は,大学生新入生を対象に食生活に対する自己効力感とう蝕経験歯(DMF歯)の有無との関連を検討することである.対象者は2011年度岡山大学新入生のうち18,19歳の2,031名(男性1,159名,女性872名)である.アンケートにより,性別,食生活,フッ化物歯面塗布経験の有無,フッ化物配合歯磨剤の使用の有無および食生活に対する自己効力感に関する質問を行い,口腔内診査でDMF歯数を調べた.DMF歯の有無によって対象者を2群に分け,各項目について比較した.さらに,ロジスティック回帰分析を行った.ロジスティック回帰分析の結果.DMF歯の有無による2群比較において統計学的に有意であった変数は,性別(OR1.325,95%CI1.099-1.596,p=0.003),フッ化物歯面塗布経験の有無(OR0.765,95%CI0.634-0.924.p=0.005).食生活に対する自己効力感(OR0.813,95%CI0.672-0.982,p=0.032)および不規則な食事時間(OR1.250,95%CI1.005-1.554,p=0.045)の4項目であった.以上より,DMF歯の有無と食生活に対する自己効力感との間に有意な関連があった.

DOI： 10.5834/jdh.62.4_403

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その他リンク： http://search.jamas.or.jp/link/ui/2012333656

早食いの是正に対するパンフレットと食事記録法の効果．

竹内倫子, 江國大輔, 友藤孝明, 森田学

口腔衛生学会雑誌 62 ( 2 ) 218 - 218 2012年

小学生における永久歯の萠出状況の報告

横井彩, 山中玲子, 江國大輔, 丸山貴之, 入江浩一郎, 遠藤康正, 水谷慎介, 友藤孝明, 森田学

岡山歯学会雑誌 30 ( 2 ) 87 - 87 2011年12月

耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

志茂加代子, 岡崎惠子, 三浦留美, 山中玲子, 水川展吉, 江国大輔, 丸山貴之, 横井彩, 仲田直樹, 武田斉子, 喜田沙音里, 森田学

岡山歯学会雑誌 30 ( 2 ) 82 - 82 2011年12月

Relationships Between Eating Habits and Periodontal Condition in University Students 国際誌

Takaaki Tomofuji, Michiko Furuta, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

JOURNAL OF PERIODONTOLOGY 82 ( 12 ) 1642 - 1649 2011年12月

記述言語：英語出版者・発行元：AMER ACAD PERIODONTOLOGY

Background: Being overweight is a risk factor for peri odontitis. Unhealthy eating habits, which can induce overweight, may be involved in the development of periodontitis in young people. The present study aims to examine the relationships among overweight, eating habits, and the periodontal condition in Japanese university students.
Methods: We conducted a cross-sectional study of 801 university students (413 males and 388 females; age range: 18 to 25 years). Patients were classified as underweight (body mass index [BMI] < 18.5 kg/m(2)), normal weight (BMI of 18.5 to 22.9 kg/m(2)), and overweight (BMI >= 23 kg/m(2)). Students completed a questionnaire including items related to eating habits and underwent oral health examinations. Patients with a community periodontal index (CPI) of 0 to 2 were considered controls, and patients with a CPI > 2 were considered to have periodontitis.
Results: The prevalence of underweight, normal weight, and overweight patients was 21%, 62%, and 17%, respectively. In overweight patients, the periodontitis risk was increased by the frequent consumption of fatty foods (adjusted odds ratio: 2.3; 95% confidence interval: 1.1 to 5.2; P < 0.05) and reduced by the frequent consumption of vegetables (adjusted odds ratio: 0.2; 95% confidence interval: 0.1 to 0.7; P < 0.01). In underweight and normal-weight groups, eating habits did not differ significantly according to the presence of periodontitis.
Conclusions: In overweight students, the frequent consumption of fatty foods and infrequent consumption of vegetables were associated with an increased risk of periodontitis. In underweight and normal-weight students, eating habits had little effect on the periodontal condition. J Periodontol 2071; 82:1642-1649.

DOI： 10.1902/jop.2011.110061

Hydrogen-rich water attenuates experimental periodontitis in a rat model 国際誌

Kenta Kasuyama, Takaaki Tomofuji, Daisuke Ekuni, Naofumi Tamaki, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY 38 ( 12 ) 1085 - 1090 2011年12月

記述言語：英語出版者・発行元：WILEY-BLACKWELL

Aim: Reactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen-rich water on a rat model of periodontitis.
Material & Methods: A ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen-rich water.
Results: The rats with periodontitis which were treated with pure water showed a time-dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis-induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen-rich water intake inhibited an increase in serum ROS level and lowered expression of 8-hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen-activated protein kinases, were less activated in periodontal lesions from hydrogen-rich water-treated rats as compared with pure water-treated rats.
Conclusion: Consuming hydrogen-rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

DOI： 10.1111/j.1600-051X.2011.01801.x

Periodontal treatment decreases plasma oxidized LDL level and oxidative stress 国際誌

Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Manabu Morita

CLINICAL ORAL INVESTIGATIONS 15 ( 6 ) 953 - 958 2011年12月

記述言語：英語出版者・発行元：SPRINGER HEIDELBERG

Periodontitis induces excessive production of reactive oxygen species in periodontal lesions. This may impair circulating pro-oxidant/anti-oxidant balance and induce the oxidation of low-density lipoprotein (LDL) in blood. The purpose of this study was to monitor circulating oxidized LDL and oxidative stress in subjects with chronic periodontitis following non-surgical periodontal treatment. Plasma levels of oxidized LDL and oxidative stress in 22 otherwise healthy non-smokers with chronic periodontitis (mean age 44.0 years) were measured at baseline and at 1 and 2 months after non-surgical periodontal treatment. At baseline, chronic periodontitis patients had higher plasma levels of oxidized LDL and oxidative stress than healthy subjects (p < 0.001). Periodontal treatment was associated with a significant reduction in plasma levels of oxidized LDL (oxLDL)(p < 0.001) and oxidative stress (p < 0.001). At 2 months after periodontal treatment, the degree of change in the oxLDL was positively correlated with that in the oxidative stress (r = 0.593, p = 0.004). These observations indicate that periodontitis patients showed higher levels of circulating oxLDL and oxidative stress than healthy subjects. In addition, improved oral hygiene and non-surgical periodontal treatment were effective in decreasing oxLDL, which was positively associated with a reduction in circulating oxidative stress.

DOI： 10.1007/s00784-010-0458-y

Stage of hepatocellular carcinoma is associated with periodontitis 国際誌

Naofumi Tamaki, Akinobu Takaki, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Daisuke Ekuni, Tetsuya Yasunaka, Kazuhide Yamamoto, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY 38 ( 11 ) 1015 - 1020 2011年11月

記述言語：英語出版者・発行元：WILEY-BLACKWELL

Aim: Periodontitis induces overproduction of reactive oxygen species (ROS). This state increases circulating ROS levels and may affect hepatocellular carcinoma (HCC). The Japan Integrated Stage (JIS) score is a novel staging system for HCC. The objective of the present study was to compare JIS scores in HCC patients with and without periodontitis.
Material and Methods: We recruited 64 HCC patients comprising 31 chronic periodontitis subjects (HCC + P) and 33 periodontally healthy controls (HCC + H). Their JIS scores were recorded. Serum levels of reactive oxygen metabolites (ROM) from HCC + P, HCC + H and healthy age-and gender-matched subjects with healthy gingiva (control, n = 15) were also assessed for circulating ROS levels.
Results: The HCC + P and HCC + H groups had similar body mass index, habitual drinking and tobacco exposure data. The HCC + P group showed higher JIS scores than the HCC + H group (p = 0.027). Both the HCC + P and HCC + H groups had higher serum levels of ROM than controls (p < 0.001), while serum levels of ROM in the HCC + P group were a further 25.8% higher than those in the HCC + H group (p < 0.001).
Conclusion: HCC patients with periodontitis had higher JIS score and circulating ROS level than HCC patients without periodontitis.

DOI： 10.1111/j.1600-051X.2011.01777.x

Relationships between periodontal inflammation, lipid peroxide and oxidative damage of multiple organs in rats

Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamotto, Tatsuo Watanabe, Manabu Morita

BIOMEDICAL RESEARCH-TOKYO 32 ( 5 ) 343 - 349 2011年10月

記述言語：英語出版者・発行元：BIOMEDICAL RESEARCH PRESS LTD

Gingival response to periodontal inflammation generates excessive lipid peroxide and such a condition may augment systemic health through increased circulating lipid peroxide. The purpose of the present study was to investigate whether the generation of lipid peroxide in periodontal inflammation could induce tissue injury in the liver, heart, kidney and brain using a rat model. Twelve Wistar rats (8 week-old male) were divided into 2 groups: the periodontal inflammation group, receiving topical application of lipopolysaccharide and proteases to the gingival sulcus for 4 weeks, and the control group using instead pyrogen-free water. After blood samples were collected, specimens from the brain, heart, liver and kidney were resected to determine the concentration of 8-hydroxydeoxyguanosine (an indicator of oxidative DNA damage). Gingival and serum levels for hexanoyl-lysine were measured to evaluate lipid peroxide. Administration of lipopolysaccharide and proteases induced periodontal inflammation, with increasing gingival and serum levels of hexanoyl-lysine. The level of 8-hydroxydeoxyguanosine increased 2.27, 2.01, 1.49 and 1.40 times in mitochondrial DNA from the liver, heart, kidney and brain of rats with periodontal inflammation, respectively. The results reveal that excessive production of lipid peroxide following periodontal inflammation is involved in oxidative DNA damage of the brain, heart, liver and kidney.

DOI： 10.2220/biomedres.32.343

Relationship between impacts attributed to malocclusion and psychological stress in young Japanese adults 国際誌

Daisuke Ekuni, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Takashi Murakami, Takashi Yamashiro, Toshio Ogura, Manabu Morita

EUROPEAN JOURNAL OF ORTHODONTICS 33 ( 5 ) 558 - 563 2011年10月

記述言語：英語出版者・発行元：OXFORD UNIV PRESS

Identifying risk factors is important to prevent a wide range of health-damaging behaviours and to improve the quality of life of young people. The aim of this study was to investigate the relationship between impacts on daily performance attributed to malocclusion and psychological stress in healthy young Japanese adults.
Medical and oral health data were collected during a cross-sectional examination conducted by the Health Service Center of Okayama University. Systemically healthy non-smoking students aged 18 and 19 years (n = 641; 329 males and 312 females) were included. Malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need (IOTN). The impacts on daily performance attributed to malocclusion and psychological stress were assessed using self-reported questionnaires, the condition-specific oral impacts on daily performances (CS-OIDP), and the Hopkins Symptoms Checklist. Mann-Whitney U- and chi-square tests and structural equation modelling (SEM) were used for statistical analysis.
Forty per cent of subjects had a malocclusion (n = 255). Subjects with impacts on daily performance had a significantly higher prevalence of malocclusion than those without impacts (P < 0.001). SEM showed that psychological stress, especially interpersonal sensitivity and depression, was significantly correlated with CS-OIDP and malocclusion. Negative impacts on daily performance attributed to malocclusion may contribute to psychological stress in young Japanese adults.

DOI： 10.1093/ejo/cjq121

Occlusal disharmony increases amyloid-β in the rat hippocampus.

Ekuni D, Tomofuji T, Irie K, Azuma T, Endo Y, Kasuyama K, Morita M

Neuromolecular Med 13 ( 3 ) 197 - 203 2011年9月

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