MISC - 江國　大輔

根面う蝕の管理と歯周病 所変われば対処・リスクも変わる?!

江國 大輔

日本歯周病学会会誌   66 ( 秋季特別 )   128 - 128   2024年10月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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口腔内のPorphyromonas gingivalis菌数と血清中抗ジンジパイン抗体価の関連性

中村 彪雅, 下津 尭子, 安居 良太, 大廣 義幸, 安田 多賀子, 丸山 貴之, 畑中 加珠, 江國 大輔, 高柴 正悟

臨床化学   53 ( Suppl.1 )   211 - 211   2024年7月

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記述言語：日本語   出版者・発行元：(一社)日本臨床化学会  

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歯科疾患の予防と健康増進 笑いも大切?!

江國 大輔

大阪府歯科医師会雑誌   ( 792 )   5 - 5   2024年5月

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記述言語：日本語   出版者・発行元：(一社)大阪府歯科医師会  

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歯科疾患の予防と健康増進 笑いも大切?!

江國 大輔

大阪府歯科医師会雑誌   ( 792 )   5 - 5   2024年5月

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記述言語：日本語   出版者・発行元：(一社)大阪府歯科医師会  

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口臭研究の新たな展開のためのヒント マイクロRNAと口臭との関係

江國 大輔

日本口臭学会会誌   15 ( 1 )   26 - 27   2024年5月

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記述言語：日本語   出版者・発行元：日本口臭学会  

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歯周病の治療と継続管理におけるブラッシングの役割について

堀田 佳美, 山本 美由希, 岩崎 美幸, 藤田 あゆみ, 宮脇 芽里, 黒瀬 真由美, 江國 大輔, 森田 学

口腔衛生学会雑誌   74 ( 増刊 )   159 - 159   2024年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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マウスモデルにおける歯周病と糖尿病がアルツハイマー病に与える影響

中原 桃子, 福原 大樹, Mohammad Nurhamim, Zhang Yixuan, 丸山 貴之, 江國 大輔

口腔衛生学会雑誌   74 ( 増刊 )   153 - 153   2024年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯周状態と唾液エクソソーム中miRNAとの関係 横断研究

澤田 ななみ, 福原 大樹, 角田 伊知郎, 中原 桃子, 外山 直樹, 横井 彩, 竹内 倫子, 丸山 貴之, 江國 大輔

口腔衛生学会雑誌   74 ( 増刊 )   141 - 141   2024年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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マウスモデルにおける歯周病と糖尿病がアルツハイマー病に与える影響

中原 桃子, 福原 大樹, Mohammad Nurhamim, Zhang Yixuan, 丸山 貴之, 江國 大輔

口腔衛生学会雑誌   74 ( 増刊 )   153 - 153   2024年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯周病の治療と継続管理におけるブラッシングの役割について

堀田 佳美, 山本 美由希, 岩崎 美幸, 藤田 あゆみ, 宮脇 芽里, 黒瀬 真由美, 江國 大輔, 森田 学

口腔衛生学会雑誌   74 ( 増刊 )   159 - 159   2024年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯周状態と唾液エクソソーム中miRNAとの関係 横断研究

澤田 ななみ, 福原 大樹, 角田 伊知郎, 中原 桃子, 外山 直樹, 横井 彩, 竹内 倫子, 丸山 貴之, 江國 大輔

口腔衛生学会雑誌   74 ( 増刊 )   141 - 141   2024年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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サルコペニアと口腔機能との関連(横断研究)

稲田 さくら, 竹内 倫子, 澤田 ななみ, 外山 直樹, 横井 彩, 丸山 貴之, 角田 伊知郎, 福原 大樹, 森田 学, 江國 大輔

口腔衛生学会雑誌   74 ( 1 )   60 - 60   2024年1月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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矯正歯科治療におけるセルフケアに対する自己効力感に関する尺度の信頼性と妥当性の検討

山地 加奈, 江國 大輔, 森田 学

口腔衛生学会雑誌   74 ( 1 )   62 - 63   2024年1月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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矯正歯科治療におけるセルフケアに対する自己効力感に関する尺度の信頼性と妥当性の検討

山地 加奈, 江國 大輔, 森田 学

口腔衛生学会雑誌   74 ( 1 )   62 - 63   2024年1月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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サルコペニアと口腔機能との関連(横断研究)

稲田 さくら, 竹内 倫子, 澤田 ななみ, 外山 直樹, 横井 彩, 丸山 貴之, 角田 伊知郎, 福原 大樹, 森田 学, 江國 大輔

口腔衛生学会雑誌   74 ( 1 )   60 - 60   2024年1月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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マウスモデルの歯周炎にBacillus subtilisが与える影響(Effects of Bacillus subtilis on periodontitis in a mouse model)

Zhang Yixuan, Mohammad Nurhamim, 中原 桃子, 福原 大樹, 丸山 貴之, 江國 大輔

岡山歯学会雑誌   42 ( 2 )   76 - 76   2023年12月

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記述言語：英語   出版者・発行元：岡山歯学会  

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令和のフッ化物応用戦略 VIVANT

江國 大輔

岡山歯学会雑誌   42 ( 2 )   79 - 79   2023年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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中高年におけるう蝕経験と唾液中の口腔内細菌叢との関係(横断研究)

横井 彩, 外山 直樹, 竹内 倫子, 江國 大輔

日本未病学会学術総会抄録集   30回   65 - 65   2023年12月

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記述言語：日本語   出版者・発行元：(一社)日本未病学会  

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マウスモデルの歯周炎にBacillus subtilisが与える影響(Effects of Bacillus subtilis on periodontitis in a mouse model)

Zhang Yixuan, Mohammad Nurhamim, 中原 桃子, 福原 大樹, 丸山 貴之, 江國 大輔

岡山歯学会雑誌   42 ( 2 )   76 - 76   2023年12月

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記述言語：英語   出版者・発行元：岡山歯学会  

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令和のフッ化物応用戦略 VIVANT

江國 大輔

岡山歯学会雑誌   42 ( 2 )   79 - 79   2023年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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高校生における第二大臼歯の萠出状態

竹内 倫子, 江國 大輔, 外山 直樹, 森田 学

日本公衆衛生学会総会抄録集   82回   473 - 473   2023年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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高校生における第二大臼歯の萠出状態

竹内 倫子, 江國 大輔, 外山 直樹, 森田 学

日本公衆衛生学会総会抄録集   82回   473 - 473   2023年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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厚生労働省「歯科疾患実態調査」の二次データ分析に見る壮年期歯科疾患対策の重要性

三宅 雅, 江國 大輔, 森田 学

日本老年医学会雑誌   60 ( Suppl. )   154 - 155   2023年5月

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記述言語：日本語   出版者・発行元：(一社)日本老年医学会  

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揮発性硫黄化合物に対する舌背への光線力学療法の効果 無作為化比較試験

江國 大輔, 横井 彩, 森田 学

日本口臭学会会誌   14 ( 1 )   27 - 27   2023年5月

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記述言語：日本語   出版者・発行元：日本口臭学会  

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厚生労働省「歯科疾患実態調査」の二次データ分析に見る壮年期歯科疾患対策の重要性

三宅 雅, 江國 大輔, 森田 学

日本老年医学会雑誌   60 ( Suppl. )   154 - 155   2023年5月

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記述言語：日本語   出版者・発行元：(一社)日本老年医学会  

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揮発性硫黄化合物に対する舌背への光線力学療法の効果 無作為化比較試験

江國 大輔, 横井 彩, 森田 学

日本口臭学会会誌   14 ( 1 )   27 - 27   2023年5月

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記述言語：日本語   出版者・発行元：日本口臭学会  

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大学生における歯周病発症と受動喫煙を含む危険因子との関係 コホート研究

内田 悠理香, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 福原 瑶子, 外山 直樹, 澤田 ななみ, 中島 千穂, 中原 桃子, 角田 伊知郎, 森田 学

口腔衛生学会雑誌   73 ( 増刊 )   149 - 149   2023年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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令和の健口戦略;基礎研究からの発信!! 次世代へのmission Transformation,Integration and Prevention

江國 大輔

口腔衛生学会雑誌   73 ( 増刊 )   92 - 92   2023年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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睡眠時ブラキシズムと関連する栄養素の探索的研究

外山 直樹, 江國 大輔, 福原 大樹, 澤田 ななみ, 森田 学

口腔衛生学会雑誌   73 ( 増刊 )   148 - 148   2023年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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睡眠時ブラキシズムとトリプトファンとの関連 横断研究

外山 直樹, 江國 大輔, 森田 学

Journal of Epidemiology   33 ( Suppl.1 )   99 - 99   2023年2月

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記述言語：日本語   出版者・発行元：(一社)日本疫学会  

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大学生における主観的口腔健康評価の傾向と,口腔健康状態および口腔保健行動との関連性

中原 桃子, 外山 直樹, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 澤田 ななみ, 中島 千穂, 森田 学

岡山歯学会雑誌   41 ( 2 )   61 - 61   2022年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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日本の大学生における歯周病の有病者率とその関連因子 10年間のデータからみる傾向

江國 大輔, 外山 直樹, 竹内 倫子, 森田 学

日本公衆衛生学会総会抄録集   81回   389 - 389   2022年9月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

老年歯科医学   37 ( 2 )   109 - 109   2022年9月

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記述言語：日本語   出版者・発行元：(一社)日本老年歯科医学会  

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地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

老年歯科医学   37 ( 2 )   109 - 109   2022年9月

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記述言語：日本語   出版者・発行元：(一社)日本老年歯科医学会  

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日本の大学生における歯周病の有病者率とその関連因子 10年間のデータからみる傾向

江國 大輔, 外山 直樹, 竹内 倫子, 森田 学

日本公衆衛生学会総会抄録集   81回   389 - 389   2022年9月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

日本老年歯科医学会総会・学術大会プログラム・抄録集   33回   O4 - 05   2022年6月

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記述言語：日本語   出版者・発行元：(一社)日本老年歯科医学会  

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地域在住高齢者における認知機能低下と口腔機能およびソーシャル・キャピタルとの関連

竹内 倫子, 澤田 ななみ, 鷲尾 憲文, 澤田 弘一, 江國 大輔, 森田 学

日本老年歯科医学会総会・学術大会プログラム・抄録集   33回   O4 - 05   2022年6月

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記述言語：日本語   出版者・発行元：(一社)日本老年歯科医学会  

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厚生労働省「歯科疾患実態調査」のヒートマップ的疾患動向分析にみる日本の医療制度改革の方向性

三宅 雅, 江國 大輔, 森田 学

日本老年医学会雑誌   59 ( Suppl. )   137 - 137   2022年5月

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記述言語：日本語   出版者・発行元：(一社)日本老年医学会  

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厚生労働省「歯科疾患実態調査」のヒートマップ的疾患動向分析にみる日本の医療制度改革の方向性

三宅 雅, 江國 大輔, 森田 学

日本老年医学会雑誌   59 ( Suppl. )   137 - 137   2022年5月

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記述言語：日本語   出版者・発行元：(一社)日本老年医学会  

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舌背への光線力学療法による口臭の改善効果の検討 ランダム化比較試験

長崎 潤一郎, 丸山 貴之, 横井 彩, 江國 大輔, 澤田 ななみ, 森田 学

口腔衛生学会雑誌   72 ( 増刊 )   81 - 81   2022年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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コロナ禍前とコロナ禍における大学生の睡眠時ブラキシズムの検討(横断研究)

江國 大輔, 丸山 貴之, 横井 彩, 福原 大樹, 外山 直樹, 澤田 ななみ, 中原 桃子, 角田 伊知郎, 森田 学

口腔衛生学会雑誌   72 ( 増刊 )   79 - 79   2022年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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睡眠時ブラキシズムの危険因子に関するコホート研究

神野 弘貴, 外山 直樹, 江國 大輔, 森田 学

口腔衛生学会雑誌   72 ( 増刊 )   79 - 79   2022年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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周術期のガム咀嚼トレーニングは胸部食道がん患者の咀嚼能力を維持・向上させる

山中 玲子, 白川 靖博, 横井 彩, 中村 吉秀, 難波 奏人, 田辺 俊介, 野間 和広, 清水 一好, 内田 悠理香, 吉冨 愛子, 丸山 貴之, 江國 大輔, 森田 学

口腔衛生学会雑誌   72 ( 増刊 )   101 - 101   2022年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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術後2週間における胃がんおよび食道がん患者の舌圧減少率と舌圧関連因子の比較

横井 彩, 秦 浩信, 藤嶋 亮太, 篠原 敏樹, 江國 大輔, 森田 学

日本摂食・嚥下リハビリテーション学会雑誌   26 ( 1 )   3 - 9   2022年4月

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記述言語：日本語   出版者・発行元：(一社)日本摂食嚥下リハビリテーション学会  

【目的】舌圧は嚥下機能の指標で、食道がん術後に低下する。その減少量は絶食期間やIntensive care unit(ICU)滞在期間に関連する。しかし、食道がんよりも嚥下機能に関わる組織への侵襲がなく、絶食期間やICU滞在日数が短い疾患の周術期における舌圧減少は不明である。胃がんの周術期における嚥下スクリーニングが注目されている。本研究の目的は、胃がん患者と食道がん患者における手術前後の舌圧減少率を比較することである。【対象と方法】対象者は、2016年1月から2017年12月までに、岡山大学病院と北海道がんセンターで手術を行い、嚥下機能評価を行った患者のうち、年齢、性別、術前舌圧、がんの進行度(ステージ)、術前Repetitive Saliva Swallowing Test(RSST)のスコアをマッチングさせた胃がん患者20名、食道がん患者20名(年齢40-88歳、男性26名、女性14名)とした。嚥下機能評価として、術前、術後1週目、2週目に舌圧とRSSTを測定した。また、舌圧に関連する因子として、年齢、性別、ステージ、術式、手術時間・出血量、挿管日数、絶食期間、発熱日数、ICU滞在期間、術後入院期間、飲酒喫煙習慣を調査した。胃がん患者と食道がん患者の周術期における舌圧の推移と術前舌圧を基準とした舌圧減少率をWilcoxonの符号付順位検定、Two-way ANOVAにて統計分析した。さらに、2群間で舌圧減少に関連する因子を比較した(Mann-Whitney U検定、χ2検定)。【結果】胃がん患者の舌圧減少率は、食道がん患者と比較し、統計学的に有意に小さかった(p=.026)。胃がん患者は、食道がん患者と比較し、周術期舌圧に影響を与える手術時間(p<.001)、絶食期間(p<.001)、ICU滞在期間(p<.001)が有意に短かった。【考察】胃がん患者の舌圧減少率が小さい理由として、胃がんの手術は、術野が口腔、咽頭、喉頭から離れており、また手術時間・絶食期間・ICU滞在期間が食道がんの手術に比べると短いことが関係している可能性がある。【結論】周術期において、胃がん患者の舌圧減少率は、食道がん患者と比較して小さかった。(著者抄録)

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修復物の変化を評価に加えたう蝕発症と曝露因子との関係 3年間のコホート研究

江國 大輔, 外山 直樹, 森田 学

Journal of Epidemiology   32 ( Suppl.1 )   168 - 168   2022年1月

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記述言語：日本語   出版者・発行元：(一社)日本疫学会  

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家庭での毎日の受動喫煙曝露は大学生の歯周病と関係する(横断研究)

江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, 外山 直樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中原 桃子, 中島 千穂, 出口 純也, 福原 瑶子, 森田 学

口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022年1月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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岡山大学新入生の口腔健康状態および主観的口腔健康評価の9年間の変化

中原 桃子, 外山 直樹, 江國 大輔, 竹内 倫子, 丸山 貴之, 横井 彩, 福原 大樹, イスラム・モハマド・モニルル, 澤田 ななみ, 中島 千穂, 出口 純也, 森田 学

口腔衛生学会雑誌   72 ( 1 )   54 - 54   2022年1月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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自立高齢者における口腔機能、栄養状態および身体的フレイルの関係

澤田 ななみ, 竹内 倫子, 江國 大輔, 森田 学

岡山歯学会雑誌   40 ( 2 )   33 - 34   2021年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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自立高齢者における口腔機能、栄養状態および身体的フレイルの関係

澤田 ななみ, 竹内 倫子, 江國 大輔, 森田 学

岡山歯学会雑誌   40 ( 2 )   33 - 34   2021年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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自立高齢者における口腔機能、栄養状態および身体的フレイルの関係 縦断調査

竹内 倫子, 江國 大輔, 森田 学

日本公衆衛生学会総会抄録集   80回   241 - 241   2021年11月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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自立高齢者における口腔機能、栄養状態および身体的フレイルの関係 縦断調査

竹内 倫子, 江國 大輔, 森田 学

日本公衆衛生学会総会抄録集   80回   241 - 241   2021年11月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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唾液中乳酸脱水素酵素(LDH)測定用簡易キットによるスコアの悪化にはセルフエフィカシーが関係する

江國 大輔, 片岡 広太, 福原 大樹, 内田 瑶子, 外山 直樹, 小林 暉政, イスラム・モハマド・モニルル, 澤田 ななみ, 森田 学

日本歯科医師会雑誌   74 ( 4 )   407 - 407   2021年7月

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記述言語：日本語   出版者・発行元：(公社)日本歯科医師会  

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口腔内局所臭気回収方法の最適化に関する研究

松本 卓也, 高橋 有弥瑠, 松尾 明彦, 江國 大輔, 森田 学

日本口臭学会会誌   12 ( 1 )   46 - 46   2021年5月

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記述言語：日本語   出版者・発行元：日本口臭学会  

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「口臭による日常生活への支障」に関連する要因

佐保 輝, 横井 彩, 丸山 貴之, 竹内 倫子, 江國 大輔, 森田 学

日本口臭学会会誌   12 ( 1 )   49 - 50   2021年5月

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記述言語：日本語   出版者・発行元：日本口臭学会  

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ヘルスリテラシーとリスク選好が定期的な歯科健診への受診意志に与える影響

角田 伊知郎, 森田 学, 江國 大輔, 丸山 貴之, 片岡 広太, 横井 彩, 福原 大樹, 福原 瑶子, 外山 直樹, 中原 桃子

口腔衛生学会雑誌   71 ( 増刊 )   88 - 88   2021年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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頭頸部がん治療による口腔機能・QOLへの影響 縦断研究

横井 彩, 丸山 貴之, 中原 桃子, 山中 玲子, 江國 大輔, 森田 学

口腔衛生学会雑誌   71 ( 増刊 )   116 - 116   2021年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Association between Sleep Quality and Duration and Periodontal Disease among University Students: A Cross-Sectional Study. 国際誌

Md Monirul Islam, Daisuke Ekuni, Naoki Toyama, Ayano Taniguchi-Tabata, Kota Kataoka, Yoko Uchida-Fukuhara, Daiki Fukuhara, Hikari Saho, Nanami Sawada, Yukiho Nakashima, Yoshiaki Iwasaki, Manabu Morita

International journal of environmental research and public health   17 ( 9 )   2020年4月

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記述言語：英語  

The purpose of this cross-sectional study was to investigate the association between sleep quality and duration, and periodontal disease among a group of young Japanese university students. First-year students (n = 1934) at Okayama University who voluntarily underwent oral health examinations were included in the analysis. Sleep quality and duration were assessed by the Japanese version of the Pittsburgh Sleep Quality Index. Dentists examined Oral Hygiene Index-Simplified (OHI-S), probing pocket depth (PPD), and percentage of sites with bleeding on probing (BOP). Periodontal disease was defined as presence of PPD ≥ 4 mm and BOP ≥ 30%. Overall, 283 (14.6%) students had periodontal disease. Poor sleep quality was observed among 372 (19.2%) students. Mean (± standard deviation) sleep duration was 7.1 ± 1.1 (hours/night). In the logistic regression analysis, periodontal disease was significantly associated with OHI-S (odds ratio [OR]: 2.30, 95% confident interval [CI]: 1.83-2.90; p < 0.001), but not sleep quality (OR: 1.09, 95% CI: 0.79-1.53; p = 0.577) or sleep duration (OR: 0.98, CI: 0.87-1.10; p = 0.717). In conclusion, sleep quality and duration were not associated with periodontal disease among this group of young Japanese university students.

DOI： 10.3390/ijerph17093034

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腸内細菌の有無が胎児内軟骨成長に与える影響

内田瑶子, 服部高子, 福原大樹, FU Shanqi, 近藤星, 桑原美穂, ISLAM Monirul, 片岡広太, 江國大輔, 久保田聡, 森田学

日本軟骨代謝学会プログラム・抄録集   33rd   2020年

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日中の咬筋の筋活動は歯周炎の進行と関係する:コホート研究

江國大輔, 加藤聖也, 川上滋央, 丸山貴之, 片岡広太, 内田瑤子, 福原大樹, 外山直樹, 皆木省吾, 森田学

日本疫学会学術総会講演集(Web)   30th   2020年

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J-GLOBAL

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歯周病ラットにおける血清miRNAの肝臓mRNAへの影響

杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

口腔衛生学会雑誌   69 ( 増刊 )   182 - 182   2019年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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ラット歯周病モデルにおける血清miRNAと肝臓への影響

杉浦 嘉雄, 米田 俊樹, 藤森 浩平, 丸山 貴之, 宮井 久敬, 小林 暉政, 江國 大輔, 友藤 孝明, 森田 学

岡山歯学会雑誌   37 ( 2 )   81 - 81   2018年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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化学放射線療法中の口腔粘膜炎管理を行った症例

益成 美保, 水野 裕文, 丸山 貴之, 横井 彩, 小林 暉政, 佐々木 禎子, 志茂 加代子, 三浦 留美, 水川 展吉, 江國 大輔, 森田 学

口腔衛生学会雑誌   68 ( 4 )   231 - 237   2018年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

頭頸部がんに対する化学放射線療法(CRT)の有害事象である口腔粘膜炎による疼痛は、患者のQOLを低下させ、がん治療の完遂を困難にする。今回、われわれは術後CRTを受けている患者に対して口腔管理を行い、口腔粘膜炎および疼痛を制御した結果、がん治療の完遂に貢献できた症例を報告する。患者は67歳、男性である。右側舌がん(T2N2bM0)に対して舌可動部半側切除術、右側頸部郭清術、遊離前外側大腿皮弁による再建術を施行した。術後の病理組織検査より切除断端陽性、頸部リンパ節の節外浸潤を認めたため、術後CRT(抗がん剤:シスプラチン、5-フルオロウラシル;2クール、強度変調放射線治療60Gy)を行うこととなった。CRT前から、口腔管理および衛生実地指導を継続して行った。CRT開始時はプラークの除去を中心とした口腔管理を行った。22Gy照射時点で口腔粘膜炎(Grade2)が発症したものの、その後軽度疼痛を認める程度で経過していた。42Gy照射時点で口腔粘膜炎の悪化(Grade3)および自発痛・接触痛を認めたため、治療完遂まで毎日、歯科衛生士がプラークの除去に加え、口腔粘膜保護を目的とした口腔管理を行った。その結果、56Gy照射時点で口腔粘膜炎は改善傾向を示し(Grade2)、疼痛も緩和され、CRT完遂に至った。術後CRT開始直後から口腔粘膜炎の状態に応じた口腔管理を行うことで、疼痛の制御が成功し、CRTの完遂に貢献できたと考えられる。(著者抄録)

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Relationship between severity of periodontitis and masseter muscle activity during waking and sleeping hours

Seiya Kato, Daisuke Ekuni, Shigehisa Kawakami, Acing Habibie Mude, Manabu Morita, Shogo Minagi

Archives of Oral Biology   90   13 - 18   2018年6月

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記述言語：英語   出版者・発行元：Elsevier Ltd  

Objective: This study was conducted to investigate whether the masseter muscle activity shows any specific pattern in relation to the severity of periodontitis. Design: Sixteen subjects with no or mild periodontitis (NMP group) and 15 subjects with moderate or severe periodontitis (MSP group) were enrolled. Plasma IgG antibody titer was examined using Porphyromonas gingivalis as a bacterial antigen. Surface electromyography (EMG) of the masseter muscles was continuously recorded using an ambulatory surface EMG recording device while patients were awake and asleep. Masseter muscle activity was analyzed using intensities of 5%–10% maximal voluntary clenching (MVC), 10%–20% MVC, and &gt
20% MVC. Furthermore, EMG levels of 20% MVC were adopted as the threshold for analysis of phasic, tonic, and mixed EMG activities. The cumulative duration of masseter muscle activity and bruxism episodes was calculated as duration per hour. Results: There was no significant difference in plasma IgG antibody titers against P. gingivalis between the NMP and MSP groups (p = 0.423). During waking hours, the duration of masseter muscle activity with an intensity of &gt
20% MVC was significantly longer in the MSP group than in the NMP group (p = 0.037). During sleeping hours, the duration of masseter muscle activity at all MVC intensities was significantly longer in the MSP group than in the NMP group (all p &lt
0.05). Additionally, the duration of phasic and mixed episodes was significantly longer in the MSP group than those in the NMP group while both awake and asleep (all p &lt
0.05). Conclusions: The results of this study suggested that masseter muscle activity might be related to the severity of periodontitis.

DOI： 10.1016/j.archoralbio.2018.02.021

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口腔内ジメチルサルファイド濃度と全身疾患および服薬との関係

横井 彩, 竹内 倫子, 宮井 久敬, 江國 大輔, 森田 学

日本口臭学会会誌   9 ( 1 )   38 - 39   2018年5月

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記述言語：日本語   出版者・発行元：日本口臭学会  

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歯周組織における加齢に伴う酸化ストレスの増加に対するコーヒー摂取の影響

小林 暉政, 東 哲司, 杉浦 嘉雄, 宮井 久敬, 米田 俊樹, 丸山 貴之, 江國 大輔, 友藤 孝明, 森田 学

口腔衛生学会雑誌   68 ( 増刊 )   182 - 182   2018年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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大学生における口腔関連QOLの変化に影響を及ぼす因子の検討 縦断研究

佐保 輝, 竹内 真由, 稲生 智秋, Monirul Islam Md, 杉浦 嘉雄, 外山 直樹, 内田 瑶子, 横井 彩, 谷口 綾乃, 片岡 広太, 米田 俊樹, 東 哲司, 江國 大輔, 森田 学

口腔衛生学会雑誌   68 ( 増刊 )   109 - 109   2018年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯科衛生士教育における模擬患者の違いによる実習効果の比較

木村 優子, 江國 大輔, 森田 学, 太田 正美

口腔衛生学会雑誌   68 ( 増刊 )   129 - 129   2018年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Unusual oral mucosal microbiota after hematopoietic cell transplantation with glycopeptide antibiotics: potential association with pathophysiology of oral mucositis 国際誌

Misato Muro, Yoshihiko Soga, Tomoko Higuchi, Kota Kataoka, Daisuke Ekuni, Yoshinobu Maeda, Manabu Morita

Folia Microbiologica   63 ( 5 )   1 - 11   2018年3月

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記述言語：英語   出版者・発行元：Springer Netherlands  

Severe oral mucositis occurs frequently in patients receiving hematopoietic stem cell transplantation (HCT). Oral mucosal bacteria can be associated with progression of oral mucositis, and systemic infection may occur via ulcerative oral mucositis. However, little information is available regarding the oral microbiota after HCT. Here, PCR-denaturing gradient gel electrophoresis (DGGE) was performed to characterize the oral mucosal microbiota, which can be affected by antibiotics, before and after HCT. Sixty reduced-intensity HCT patients were enrolled. Three patients with the least antibiotic use (quinolone prophylaxis and/or β-lactam monotherapy group) and three patients with the most antibiotic use (β-lactam-glycopeptide combination therapy group) were selected. Bacterial DNA samples obtained from the oral mucosa before and after HCT were subjected to PCR-DGGE. The trajectory of oral mucositis was evaluated. The oral mucosal microbiota in the β-lactam-glycopeptide combination therapy group was different from that in the quinolone prophylaxis and/or β-lactam monotherapy group, and Staphylococcus spp. and Enterococcus spp. were identified. Lautropia mirabilis was dominant in one patient. Ulcerative oral mucositis was observed only in the β-lactam-glycopeptide combination therapy group. In conclusion, especially with the use of strong antibiotics, such as glycopeptides, the oral mucosal microbiota differed completely from that under normal conditions and consisted of Staphylococcus spp., Enterococcus spp., and unexpectedly L. mirabilis. The normal oral microbiota consists not only of bacteria, but these unexpected bacteria could be involved in the pathophysiology as well as systemic infection via oral mucositis. Our results can be used as the basis for future studies in larger patient populations.

DOI： 10.1007/s12223-018-0596-1

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Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium

Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Daiki Fukuhara, Yoko Uchida, Kota Kataoka, Shuichiro Kobayashi, Takeshi Kikuchi, Akio Mitani, Yoshihiro Shimazaki, Manabu Morita

Archives of Oral Biology   87   72 - 78   2018年3月

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記述言語：英語   出版者・発行元：Elsevier Ltd  

Objective Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. Design GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. Results SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P &lt
0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P &lt
0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups. Conclusions SPF mice induced an age-related increase in CD4+ T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4+ T cell migration and cytokine expression.

DOI： 10.1016/j.archoralbio.2017.12.007

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職域における早食い、口腔内状態およびメタボリックシンドローム発症との関連

横井 彩, 江國 大輔, 米田 俊樹, 森田 学

口腔衛生学会雑誌   68 ( 1 )   9 - 14   2018年1月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

メタボリックシンドローム(Metabolic Syndrome:MetS)のリスク因子として早食いが挙げられる。また近年、MetsSは歯の喪失やう蝕など口腔内状態との関連も注目されている。しかし、MetS、早食いおよび口腔内状態を総合的に検討した報告は少ない。そこで、本縦断研究は、職域における早食いおよび口腔内状態とMetS発症との関連について検討することを目的とした。対象者は、某事業所における平成24・27年度定期健診をどちらも受診した従業員のうち、平成24年度MetSに該当しなかった男性114名(36〜63歳)を分析対象者とした。診査項目は、身長、体重、腹囲、血圧、空腹時血糖値、中性脂肪、HDLコレステロール値、口腔内状態、生活習慣(早食いの有無、食・運動・飲酒・喫煙習慣)とした。平成27年度健診結果から、MetS発症群と非発症群に分け、早食いの習慣および口腔内状態との関連を分析した。統計分析にはχ2検定、Fisherの直接法およびMann-Whitney U検定を用いた。3年後にMetSを発症した者(16名)は、全員早食いの自覚があった。また、MetS発症において、早食いと腹囲異常による相加効果がみられた。結論として、早食いで腹囲異常のある者はMetS発症のリスクが高いことがわかった。一方で、口腔内状態との直接的な関連はみられなかった。(著者抄録)

DOI： 10.5834/jdh.68.1_9

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J00440&link_issn=&doc_id=20180207240002&doc_link_id=%2Fdk1dehea%2F2018%2F006801%2F002%2F0009-0014%26dl%3D0&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdk1dehea%2F2018%2F006801%2F002%2F0009-0014%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

Type D personality and periodontal disease in university students: A prospective cohort study. 国際誌

Mizutani S, Ekuni D, Yamane-Takeuchi M, Azuma T, Taniguchi-Tabata A, Tomofuji T, Iwasaki Y, Morita M

J Health Psychol   23 ( 5 )   754 - 762   2018年

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記述言語：英語  

DOI： 10.1177/1359105316668668

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The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial 国際誌

Hirofumi Mizuno, Daisuke Ekuni, Takayuki Maruyama, Kota Kataoka, Toshiki Yoneda, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Jun Wada, Manabu Morita

PLOS ONE   12 ( 11 )   25 - 36   2017年11月

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記述言語：英語   出版者・発行元：PUBLIC LIBRARY SCIENCE  

Aim
The purpose of this study was to investigate the effects of non-surgical periodontal treatment on hemoglobinA1c (HbA1c) levels, oxidative stress balance and quality of life (QOL) in patients with type 2 diabetes mellitus (T2DM) compared to no periodontal treatment (simple oral hygiene instructions only).
Methods
The design was a 6-month, single-masked, single center, randomized clinical trial. Patients had both T2DM and chronic periodontitis. Forty participants were enrolled between April 2014 and March 2016 at the Nephrology, Diabetology and Endocrinology Department of Okayama University Hospital. The periodontal treatment group (n = 20) received non-surgical periodontal therapy, including scaling and root planing plus oral hygiene instructions, and consecutive supportive periodontal therapy at 3 and 6 months. The control group (n = 17) received only oral hygiene instructions without treatment during the experimental period. The primary study outcome was the change in HbA1c levels from baseline to 3 months. Secondary outcomes included changes in oxidative stress balance (Oxidative-INDEX), the Diabetes Therapy-Related QOL and clinical periodontal parameters from baseline to 3 months and baseline to 6 months.
Results
Changes in HbA1c in the periodontal treatment group were not significantly different with those in the control group at 3 and 6 months. Systemic oxidative stress balance and QOL significantly improved in the periodontal treatment group compared to the control group at 3 months. In the subgroup analysis (moderately poor control of diabetes), the decrease in HbA1c levels in the periodontal treatment group was greater than that in the control group at 3 months but not significant.
Conclusions
In T2DM patients, non-surgical periodontal treatment improved systemic oxidative stress balance and QOL, but did not decrease HbA1c levels at 3 months follow-up.

DOI： 10.1371/journal.pone.0188171

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大学生における歯周炎と学部系統との関連

谷口 綾乃, 江國 大輔, 竹内 真由, 森田 学

日本公衆衛生学会総会抄録集   76回   464 - 464   2017年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats 国際誌

Hisataka Miyai, Takayuki Maruyama, Takaaki Tomofuji, Toshiki Yoneda, Tetsuji Azuma, Hirofumi Mizuno, Yoshio Sugiura, Terumasa Kobayashi, Daisuke Ekuni, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   82   247 - 255   2017年10月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats.
Design: Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus.
Results: After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P &lt; 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P &lt; 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P &lt; 0.05).
Conclusions: Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

DOI： 10.1016/j.archoralbio.2017.06.025

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大学生における歯ぎしりの自覚と不正咬合との関連 前向きコホート研究

外山 直樹, 江國 大輔, 入江 浩一郎, 竹内 真由, 谷口 綾乃, 森田 学

日本公衆衛生学会総会抄録集   76回   317 - 317   2017年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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Hyperlipidemia is involved in apoptosis in rat submandibular glands 国際誌

Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Toshiki Yoneda, Yoshihiro Shimazaki, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   81   136 - 140   2017年9月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: The aim of the present study was to investigate the effects of hyperlipidemia on histological changes and apoptosis in submandibular glands using apolipoprotein E (apoE)-deficient rats.
Design: Histopathological findings related to induced apoptosis in the submandibular glands were compared between apoE-deficient rats (n = 6; male; age, 16 weeks) and the corresponding wild-type rats (n = 6).
Results: ApoE-deficient rats showed significantly higher plasma levels of oxidized low-density lipoprotein (LDL), total cholesterol, very LDL and LDL, and lower plasma levels of high-density lipoprotein when compared to control rats (P &lt; 0.05). Lipid deposition in the submandibular gland was observed in apoE-deficient rat group and in none of the control group. Significant increases in vacuolization and apoptosis in acinar cells were observed in apoE-deficient rats, as compared to control rats (P &lt; 0.05). The number of active caspase-3-positive cells was also higher in the apoE-deficient rat group when compared with the control group (P &lt; 0.01).
Conclusions: According to our results, hyperlipidemia induced apoptosis in apoE-deficient rat submandibular glands. Oxidized LDL generation in case of hyperlipidemia may trigger off a reaction of apoptotic acinar cells with vacuolization in the submandibular glands.

DOI： 10.1016/j.archoralbio.2017.05.004

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大学病院に勤務する医療技術部門職員および事務部職員における歯科用語の認知度の違い

城本 ゆき子, 三浦 留美, 高坂 由紀奈, 江國 大輔, 佐々木 朗, 飯田 征二

日本歯科衛生学会雑誌   12 ( 1 )   157 - 157   2017年8月

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記述言語：日本語   出版者・発行元：日本歯科衛生学会  

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勤務形態が口腔保健行動および口腔状態に与える影響

外山 直樹, 入江 浩一郎, 谷口 綾乃, 江國 大輔, 友藤 孝明, 森田 学

口腔衛生学会雑誌   67 ( 3 )   206 - 206   2017年7月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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抗がん剤投与ラットへのカテキン局所塗布による抗酸化作用の検討

小林 暉政, 宮井 久敬, 丸山 貴之, 友藤 孝明, 東 哲司, 米田 俊樹, 杉浦 嘉雄, 江國 大輔, 森田 学

口腔衛生学会雑誌   67 ( 3 )   203 - 203   2017年7月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Associations between dental knowledge, source of dental knowledge and oral health behavior in Japanese university students: A cross-sectional study 国際誌

Ayano Taniguchi-Tabata, Daisuke Ekuni, Shinsuke Mizutani, Mayu Yamane-Takeuchi, Kota Kataoka, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

PLOS ONE   12 ( 6 )   1 - 12   2017年6月

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記述言語：英語   出版者・発行元：PUBLIC LIBRARY SCIENCE  

The aim of this study was to investigate the associations between dental knowledge, the source of dental knowledge and oral health behavior in a group of students at a university in Japan. A total of 2,220 university students (1,276 males, 944 females) volunteered to undergo an oral examination and answer a questionnaire. The questionnaire assessed dental knowledge, the source of dental knowledge and oral health behavior (e.g., daily frequency of tooth brushing, use of dental floss and regular dental checkups). The odds ratio and 95% confidence interval for oral health behavior based on dental knowledge and source of dental knowledge were calculated using logistic regression models. Of the participants, 1,266 (57.0%) students obtained dental knowledge from dental clinics, followed by school (39.2%) and television (29.1%). Logistic regression analyses indicated that use of dental floss was significantly associated with source of dental knowledge from dental clinics (P = 0.006). Receiving regular dental checkups was significantly associated with source of dental knowledge; the positive source was dental clinic (P &lt; 0.001) and the negative sources were school (P = 0.004) and television (P = 0.018). Dental clinic was the most common source of dental knowledge and associated with better oral health behavior among the Japanese university students in this study.

DOI： 10.1371/journal.pone.0179298

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Expression of Salivary miR-203a-3p Was Related with Oral Health-Related Quality of Life in Healthy Volunteers 国際誌

Terumasa Kobayashi, Takaaki Tomofuji, Tatsuya Machida, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Akiko Hirose, Manabu Morita

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   18 ( 6 )   372 - 373   2017年6月

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記述言語：英語   出版者・発行元：MDPI AG  

Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary miRNAs expression differs according to OHRQoL in healthy volunteers. Forty-six volunteers (median age, 23.0 years) were recruited, and their OHRQoL was assessed using the Japanese version of the Oral Health Impact Profile (OHIP-J). Then, we compared salivary microRNA profiles of the high-OHRQoL group (&lt;= 25th percentile score of OHIP-J) and the low-OHRQoL group (&gt;= 75th percentile score of OHIP-J) using the polymerase chain reaction (PCR) array and the quantitative real-time PCR. There were no significant differences between the two groups in terms of oral health status. In the PCR array, miR-203a-3p and miR-30b-5p were significantly more expressed in the low-OHRQoL group (p &lt; 0.05). Quantitative real-time PCR assay also showed that miR-203a-3p was more highly expressed in the low-OHRQoL group than in the high-OHRQoL group (p &lt; 0.05). These observations suggest that expression of salivary miR-203a-3p was related with OHRQoL in healthy volunteers.

DOI： 10.3390/ijms18061263

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Association between periodontitis and prognosis of pancreatobiliary tract cancer: A pilot study. 国際誌

Takayuki Maruyama, Takaaki Tomofuji, Tatsuya Machida, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Toshiki Yoneda, Hisataka Miyai, Hirofumi Mizuno, Daisuke Ekuni, Hiroyuki Okada, Manabu Morita

Molecular and clinical oncology   6 ( 5 )   683 - 687   2017年5月

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記述言語：英語  

Several studies have indicated that periodontitis is a risk factor for cancer. However, the association between periodontitis and the prognosis of pancreatobiliary tract cancer remains unclear. The aim of this pilot study was to investigate the association between periodontitis and prognosis of pancreatobiliary tract cancer. A total of 22 patients diagnosed with pancreatobiliary tract cancer were analyzed. Oral health status, including severity of periodontitis, general health status and biochemical serum markers were evaluated. The Kaplan-Meier method and Cox proportional hazards model were used to assess factors affecting the prognosis of pancreatobiliary tract cancer. The Kaplan-Meier analysis demonstrated that low body mass index, high concentration of serum C-reactive protein (CRP) and severe periodontitis were significant prognostic factors for survival rate. The Cox proportional hazards model revealed that serum carbohydrate antigen 19-9 concentration [hazard ratio (HR)=1.002; 95% confidence interval (CI): 1.000-1.004] and serum CRP concentration (HR=2.57; 95% CI: 1.15-5.74) were significantly associated with the prognosis of pancreatobiliary tract cancer. In addition, cancer patients with severe periodontitis had higher serum CRP concentrations compared with those without severe periodontitis. Therefore, severe periodontitis indirectly affected the prognosis of pancreatobiliary tract cancer through promoting systemic inflammation.

DOI： 10.3892/mco.2017.1220

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唾液中microRNAと歯周病の重症度との関連

藤森 浩平, 米田 俊樹, 友藤 孝明, 江國 大輔, 東 哲司, 丸山 貴之, 町田 達哉, 水野 裕文, 森田 学

口腔衛生学会雑誌   67 ( 増刊 )   168 - 168   2017年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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2型糖尿病患者における非外科的歯周治療による酸化ストレスの改善 ランダム化比較試験

杉浦 嘉雄, 水野 裕文, 福原 大樹, 丸山 貴之, 江國 大輔, 米田 俊樹, 片岡 広太, 川端 勇也, 水谷 慎介, 友藤 孝明, 森田 学

口腔衛生学会雑誌   67 ( 増刊 )   119 - 119   2017年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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口腔衛生学分野のテーマをアウトカム基盤型教育で行うには 予防歯科のアウトカム基盤型教育

江國 大輔

口腔衛生学会雑誌   67 ( 増刊 )   77 - 78   2017年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet 国際誌

Toshiki Yoneda, Takaaki Tomofuji, Muneyoshi Kunitomo, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Tatsuya Machida, Hisataka Miyai, Kouhei Fujimori, Manabu Morita

NUTRIENTS   9 ( 1 )   830 - 833   2017年1月

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記述言語：英語   出版者・発行元：MDPI AG  

Obesity induces gingival oxidative stress, which is involved in the progression of alveolar bone resorption. The antioxidant effect of hydrogen-rich water may attenuate gingival oxidative stress and prevent alveolar bone resorption in cases of obesity. We examined whether hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption in obese rats fed a high-fat diet. Male Fischer 344 rats (n = 18) were divided into three groups of six rats each: a control group (fed a regular diet and drinking distilled water) and two experimental groups (fed a high-fat diet and drinking distilled water or hydrogen-rich water). The level of 8-hydroxydeoxyguanosine was determined to evaluate oxidative stress. The bone mineral density of the alveolar bone was analyzed by micro-computerized tomography. Obese rats, induced by a high-fat diet, showed a higher gingival level of 8-hydroxydeoxyguanosine and a lower level of alveolar bone density compared to the control group. Drinking hydrogen-rich water suppressed body weight gain, lowered gingival level of 8-hydroxydeoxyguanosine, and reduced alveolar bone resorption in rats on a high-fat diet. The results indicate that hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption by limiting obesity.

DOI： 10.3390/nu9010064

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ラット歯周炎モデルに対する天然植物由来成分配合歯磨剤の効果.

米田 俊樹, 東 哲司, 友藤 孝明, 江國 大輔, 片岡 広太, 山根 真由, 川端 勇也, 國友 宗義, 森田 学

口腔衛生学会雑誌   67 ( 1 )   11 - 17   2017年

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DOI： 10.5834/jdh.67.1_11

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Validity of a New Kit Measuring Salivary Lactate Dehydrogenase Level for Screening Gingivitis 国際誌

Daisuke Ekuni, Mayu Yamane-Takeuchi, Kota Kataoka, Aya Yokoi, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoko Uchida, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Manabu Morita

DISEASE MARKERS   2017   2434 - 2441   2017年

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記述言語：英語   出版者・発行元：HINDAWI LTD  

Aim. The aim of this study was to determine the usefulness of a new kit that can evaluate salivary lactate dehydrogenase (LD) level in real time for screening gingivitis. Materials and Methods. The study included 70 systemic healthy volunteers [29 males and 41 females; mean age +/- SD: 24.1 +/- 2.6 years]. Resting saliva was collected from each participant and LD level was evaluated in real time using the kit (a color-changing sheet with an integer scale ranging from 1 to 10). A dentist measured probing pocket depth, clinical attachment level, and the proportion of sites with bleeding on probing (% BOP) at six sites on all teeth. Gingivitis was diagnosed when the BOP value was &gt;= 20%. Results. Salivary LD level was positively correlated with mean % BOP (odds ratio: 1.47, 95% confidence interval: 1.132-1.916, and P &lt; 0.001) in a logistic regression model. The sensitivity and specificity of the kit were 0.89 and 0.98, respectively, at a cut-off value of 8.0 for LD level. Conclusions. The new kit for measurement of salivary LD level may be a useful tool to screen for gingivitis in young adults, which contributes to early detection of future periodontitis.

DOI： 10.1155/2017/9547956

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動機づけ面接法(MI)により高齢者の口腔衛生の改善ができた1症例.

千神 八重子, 江國 大輔, 森田 学

口腔衛生学会雑誌   67 ( 1 )   29 - 34   2017年

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DOI： 10.5834/jdh.67.1_29

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Associations among oral health-related quality of life, subjective symptoms, clinical status, and self-rated oral health in Japanese university students: a cross-sectional study 国際誌

Mayu Yamane-Takeuchi, Daisuke Ekuni, Shinsuke Mizutani, Kota Kataoka, Ayano Taniguchi-Tabata, Tetsuji Azuma, Michiko Furuta, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

BMC ORAL HEALTH   16 ( 1 )   127 - 127   2016年11月

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記述言語：英語   出版者・発行元：BIOMED CENTRAL LTD  

Background: The present study aimed to elucidate the associations among self-rated oral health, clinical oral health status, oral health behaviors, subjective oral symptoms, and oral health-related quality of life (OHRQoL) in a group of Japanese university students.
Methods: Of 2051 participants, 2027 (98.83%) students received an optional oral examination and answered a questionnaire including items regarding age, sex, self-rated oral health, oral health behaviors, subjective oral symptoms, and OHRQoL [The Oral Health Impact Profile (OHIP)-14]. On oral examination, the decayed, missing, and filled teeth (DMFT) score, Community Periodontal Index (CPI), the percentage of teeth showing bleeding on probing (%BOP), and malocclusion were recorded. Structural equation modelling (SEM) analysis was used to test associations.
Results: The mean score (+/- SD) of OHIP-14 was 1.92 +/- 5.47. In the SEM analysis, the final model showed that self-rated oral health, oral pain, malocclusion, and the DMFT score were directly associated with the OHRQoL, and subjective symptoms of temporomandibular disorders (TMD) and recurrent aphthous stomatitis were both directly and indirectly associated (p &lt; 0.05). CPI, %BOP, and oral health behaviors were excluded from the final model.
Conclusions: OHRQoL was associated with self-related oral health, subjective symptoms of TMD, oral pain and stomatitis, DMFT, and malocclusion in this group of Japanese university students.

DOI： 10.1186/s12903-016-0322-9

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Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice 国際誌

Kota Kataoka, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Muneyoshi Kunitomo, Yoko Uchida, Daiki Fukuhara, Manabu Morita

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   17 ( 11 )   2016年11月

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記述言語：英語   出版者・発行元：MDPI AG  

The aim of this study was to investigate whether a Keap1-dependent oxidative stress detector-luciferase (OKD-LUC) mouse model would be useful for the visualization of oxidative stress induced by experimental periodontitis. A ligature was placed around the mandibular first molars for seven days to induce periodontitis. Luciferase activity was measured with an intraperitoneal injection of D-luciferin on days 0, 1, and 7. The luciferase activity in the periodontitis group was significantly greater than that in the control group at seven days. The expressions of heme oxygenase-1 (HO-1) and malondialdehyde in periodontal tissue were significantly higher in the periodontitis group than in the control group. Immunofluorescent analysis confirmed that the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) occurred more frequently in the periodontitis group than in the control group. This study found that under oxidative stress induced by experimental periodontitis, the Nrf2/antioxidant defense pathway was activated and could be visualized from the luciferase activity in the OKD-LUC model. Thus, the OKD-LUC mouse model may be useful for exploring the mechanism underlying the relationship between the Nrf2/antioxidant defense pathway and periodontitis by enabling the visualization of oxidative stress over time.

DOI： 10.3390/ijms17111907

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miR-1246 and miR-4644 in salivary exosome as potential biomarkers for pancreatobiliary tract cancer 国際誌

Tatsuya Machida, Takaaki Tomofuji, Takayuki Maruyama, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Hisataka Miyai, Hirofumi Mizuno, Hironari Kato, Koichiro Tsutsumi, Daisuke Uchida, Akinobu Takaki, Hiroyuki Okada, Manabu Morita

ONCOLOGY REPORTS   36 ( 4 )   2375 - 2381   2016年10月

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記述言語：英語   出版者・発行元：SPANDIDOS PUBL LTD  

Pancreatobiliary tract cancer is a highly fatal cancer. Detection of pancreatobiliary tract cancer is difficult because it lacks typical clinical symptoms and because of its anatomical location. Biomarker discovery is therefore important to detect pancreatobiliary tract cancer in its early stage. A study demonstrated that expression levels of miR-1246, miR-3976, miR-4306, and miR-4644 in serum exosomes were higher in pancreatic cancer patients than these levels in healthy control participants. Supposing that microRNA (miRNA) expression profiles in saliva are similar to those in serum, four miRNAs (miR-1246, miR-3976, miR-4306, and miR-4644) in salivary exosomes may also be useful for detection of pancreatobiliary tract cancer. In this study, it was examined whether these miRNAs could be used as biomarkers for pancreatobiliary tract cancer. Twelve pancreatobiliary tract cancer patients and 13 healthy control participants were analyzed as a cancer and a control group, respectively. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. Using quantitative real-time PCR (RT-qPCR), the relative expression ratios of miR-1246 and miR-4644 were significantly higher in the cancer group than these ratios in the control group. Receiver operating characteristic (ROC) curves were constructed to analyze the discrimination power of these miRNAs. For miR-1246, the results yielded an area under the curve (AUC) of 0.814 (P=0.008). For miR-4644, the results yielded an AUC of 0.763 (P=0.026). For the combination of miR-1246 and miR-4644, the results yielded an increased AUC of 0.833 (P=0.005). This pilot study suggests that miR-1246 and miR-4644 in salivary exosomes could be candidate biomarkers for pancreatobiliary tract cancer.

DOI： 10.3892/or.2016.5021

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MicroRNAs as serum biomarkers for periodontitis 国際誌

Takaaki Tomofuji, Toshiki Yoneda, Tatsuya Machida, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Takayuki Maruyama, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY   43 ( 5 )   418 - 425   2016年5月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Aim: Studies demonstrated that periodontitis modulates microRNA (miRNAs) expression rates in periodontal tissue. However, the relationship between periodontitis and miRNAs profile in circulation remains unclear. In this study, we investigated the effects of periodontitis on serum miRNAs profile in a rat model.
Material and Methods: Male Wistar rats (n=32, 8weeks old) were divided into four groups of eight rats each. The control groups received no treatment for 2 or 4weeks. In the other two groups, periodontitis was ligature induced for 2 or 4weeks. Serum miRNAs expression profiles of each group were compared.
Results: Ligation around teeth induced periodontal inflammation at 2weeks and periodontal tissue destruction at 4weeks. Microarray results showed that 25 miRNAs were expressed with a &lt;0.5 or &gt;2 difference between the control and periodontitis groups at 4weeks. Results of real-time PCR revealed that the periodontitis group up-regulated expression rates of serum miR-207 and miR-495 at 2weeks, and miR-376b-3p at 4weeks (p&lt;0.05).
Conclusion: Serum miRNAs (miR-207, miR-495, and miR-376b-3p) could be valuable biomarkers for periodontitis.

DOI： 10.1111/jcpe.12536

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食道癌の術後患者に対する歯科専門職による口腔のケアの効果

水野 裕文, 水谷 慎介, 丸山 貴之, 横井 彩, 谷口 彩乃, 山中 玲子, 江國 大輔, 森田 学

口腔衛生学会雑誌   66 ( 2 )   219 - 219   2016年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯肉炎のスクリーニングにおける唾液中LDH簡易測定キットの有効性

吉田 宗生, 加藤 壮真, 山根 真由, 江國 大輔, 横井 彩, 丸山 貴之, 山中 玲子, 友藤 孝明, 森田 学

口腔衛生学会雑誌   66 ( 2 )   294 - 294   2016年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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口腔内アセトアルデヒド濃度と舌背の細菌数との関係

加藤 壮真, 吉田 宗生, 横井 彩, 丸山 貴之, 江國 大輔, 山根 真由, 山中 玲子, 友藤 孝明, 森田 学

口腔衛生学会雑誌   66 ( 2 )   296 - 296   2016年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯科衛生士教育における保護者を模擬患者とした実習の取り組みと効果

木村 優子, 江國 大輔, 森田 学, 太田 正美

口腔衛生学会雑誌   66 ( 2 )   245 - 245   2016年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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塩化セチルピリジニウム配合トローチ剤の口臭予防効果

水谷 慎介, 友藤 孝明, 片岡 広太, 川端 勇也, 江國 大輔, 森田 学

日本口臭学会会誌   7 ( 1 )   54 - 55   2016年4月

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記述言語：日本語   出版者・発行元：日本口臭学会  

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Relationship Between Prehypertension/Hypertension and Periodontal Disease: A Prospective Cohort Study 国際誌

Yuya Kawabata, Daisuke Ekuni, Hisataka Miyai, Kota Kataoka, Mayu Yamane, Shinsuke Mizutani, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

AMERICAN JOURNAL OF HYPERTENSION   29 ( 3 )   388 - 396   2016年3月

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記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

BACKGROUND
Most cross-sectional studies have found a significant positive relationship between periodontal disease and prehypertension/hypertension. However, these studies had limitations and there are few prospective cohort studies in young adults. The purpose of this prospective cohort study was to investigate whether periodontal disease was related to prehypertension/hypertension in Japanese university students.
METHODS
Students (n = 2,588), who underwent health examinations before entering university and before graduation, were included in the analysis. The association between periodontal disease such as the percentage of bleeding on probing (BOP) and community periodontal index (CPI) scores, and change in blood pressure status was determined.
RESULTS
At the reexamination, the numbers of participants with prehypertension (systolic blood pressure 120-139 mm Hg or diastolic blood pressure 80-89 mm Hg) and hypertension (&gt;= 140/90 mm Hg) were 882 (34.1%) and 109 (4.2%), respectively. In a logistic regression model, the risk of hypertension was significantly associated with male (odds ratio (OR): 6.31; 95% confidence interval (CI): 2.63-15.13; P &lt; 0.001), no habitual physical activity at baseline (OR: 2.90; 95% CI: 1.56-5.38; P &lt; 0.01) and periodontal disease defined as the presence of both probing pocket depth (PPD) &gt;= 4 m and BOP &gt;= 30% at baseline (OR: 2.74; 95% CI: 1.19-6.29; P = 0.02) in participants with prehypertension at baseline. On the other hand, the risk of prehypertension was not associated with presence of periodontal disease (OR: 0.93; 95% CI: 0.51-1.70; P = 0.82).
CONCLUSION
In the short-term prospective cohort study, a significant association between presence of periodontal disease and hypertension was observed in Japanese university students.

DOI： 10.1093/ajh/hpv117

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Association between Knowledge about Comprehensive Food Education and Increase in Dental Caries in Japanese University Students: A Prospective Cohort Study 国際誌

Muneyoshi Kunitomo, Daisuke Ekuni, Shinsuke Mizutani, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Mayu Yamane, Kota Kataoka, Ayano Taniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoshiaki Iwasaki, Manabu Morita

NUTRIENTS   8 ( 3 )   114 - 114   2016年3月

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記述言語：英語   出版者・発行元：MDPI AG  

In Japan, comprehensive food education (shokuiku) programs are carried out with the aim of improving dietary practices and thereby reducing the incidence of lifestyle-related diseases, including dental caries. The purpose of this prospective cohort study was to investigate the association between knowledge about shokuiku and the increase in dental caries among Japanese university students who had attended a shokuiku program while in junior/senior high school. A total of 562 students volunteered to undergo oral examinations over a three-year follow-up period, during which the number of cases of dental caries were recorded. Additional information was collected using a questionnaire survey regarding knowledge about shokuiku, dietary habits, and oral health behaviors. In logistic regression analysis, males who lacked knowledge about shokuiku had significantly higher odds for dental caries than those who did not (odds ratio (OR), 2.00; 95% confidence interval (CI), 1.12-3.58; p = 0.019). On the other hand, among females, those who frequently consumed sugar-sweetened soft drinks had significantly higher odds for dental caries than those who did not (OR, 1.89; 95% CI, 1.05-3.42; p = 0.035). These results suggest that having no knowledge about shokuiku is associated with a risk of increase in dental caries in Japanese male university students.

DOI： 10.3390/nu8030114

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Relationship Between Prehypertension/Hypertension and Periodontal Disease: A Prospective Cohort Study 国際誌

Yuya Kawabata, Daisuke Ekuni, Hisataka Miyai, Kota Kataoka, Mayu Yamane, Shinsuke Mizutani, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

AMERICAN JOURNAL OF HYPERTENSION   29 ( 3 )   388 - 396   2016年3月

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記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

BACKGROUND
Most cross-sectional studies have found a significant positive relationship between periodontal disease and prehypertension/hypertension. However, these studies had limitations and there are few prospective cohort studies in young adults. The purpose of this prospective cohort study was to investigate whether periodontal disease was related to prehypertension/hypertension in Japanese university students.
METHODS
Students (n = 2,588), who underwent health examinations before entering university and before graduation, were included in the analysis. The association between periodontal disease such as the percentage of bleeding on probing (BOP) and community periodontal index (CPI) scores, and change in blood pressure status was determined.
RESULTS
At the reexamination, the numbers of participants with prehypertension (systolic blood pressure 120-139 mm Hg or diastolic blood pressure 80-89 mm Hg) and hypertension (&gt;= 140/90 mm Hg) were 882 (34.1%) and 109 (4.2%), respectively. In a logistic regression model, the risk of hypertension was significantly associated with male (odds ratio (OR): 6.31; 95% confidence interval (CI): 2.63-15.13; P &lt; 0.001), no habitual physical activity at baseline (OR: 2.90; 95% CI: 1.56-5.38; P &lt; 0.01) and periodontal disease defined as the presence of both probing pocket depth (PPD) &gt;= 4 m and BOP &gt;= 30% at baseline (OR: 2.74; 95% CI: 1.19-6.29; P = 0.02) in participants with prehypertension at baseline. On the other hand, the risk of prehypertension was not associated with presence of periodontal disease (OR: 0.93; 95% CI: 0.51-1.70; P = 0.82).
CONCLUSION
In the short-term prospective cohort study, a significant association between presence of periodontal disease and hypertension was observed in Japanese university students.

DOI： 10.1093/ajh/hpv117

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口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

日本化学療法学会雑誌   64 ( 2 )   314 - 315   2016年3月

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記述言語：日本語   出版者・発行元：(公社)日本化学療法学会  

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口腔ケアの感染症予防・治療への有効性 上部消化癌患者と健常者における舌苔と口腔内アセトアルデヒド濃度との関連

山中 玲子, 横井 彩, 丸山 貴之, 江國 大輔, 森田 学

感染症学雑誌   90 ( 2 )   168 - 169   2016年3月

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記述言語：日本語   出版者・発行元：(一社)日本感染症学会  

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Type D personality and periodontal disease in university students: A prospective cohort study.

Mizutani S, Ekuni D, Yamane-Takeuchi M, Azuma T, Taniguchi-Tabata A, Tomofuji T, Iwasaki Y, Morita M

J Health Psychol   1 - 9   2016年

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造血幹細胞移植後に起こる口腔粘膜上細菌叢の変化-PCR-DGGE法を用いた解析

室美里, 室美里, 曽我賢彦, 片岡広太, 江國大輔, 飯田征二, 森田学

日本造血細胞移植学会総会プログラム・抄録集   38th   2016年

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大学生における自覚的口腔健康観と口腔内の臨床所見・口腔保健行動との関連

山根 真由, 江國 大輔, 友藤 孝明, 森田 学

日本公衆衛生学会総会抄録集   74回   442 - 442   2015年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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MicroRNAs in Salivary Exosome as Potential Biomarkers of Aging 国際誌

Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Takayuki Maruyama, Toshiki Yoneda, Yuya Kawabata, Hirofumi Mizuno, Hisataka Miyai, Muneyoshi Kunitomo, Manabu Morita

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   16 ( 9 )   21294 - 21309   2015年9月

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記述言語：英語   出版者・発行元：MDPI AG  

The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.

DOI： 10.3390/ijms160921294

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Gingival condition and tooth-brushing behavior after alcohol consumption

S. Mizutani, D. Ekuni, T. Tomofuji, M. Yamane, T. Azuma, Y. Iwasaki, M. Morita

JOURNAL OF PERIODONTAL RESEARCH   50 ( 4 )   494 - 499   2015年8月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and ObjectiveVarious studies have reported the relationship between alcohol consumption and gingival condition. However, they focus on the direct effects of alcohol consumption or alcohol sensitivity on gingival condition, and it is unclear how oral health behaviors relate these relationships. The aims of this study were to assess the inter-relationships between gingival condition, tooth-brushing behavior after drinking alcohol and alcohol sensitivity in university students who drink more than once per week on average.
Material and MethodsA total of 808 students (541 males, 267 females) that habitually consume alcohol were analyzed. The disease activity of gingival condition was assessed as the percentage of bleeding on probing (%BOP). Additional information regarding alcohol sensitivity and oral health behaviors, including tooth-brushing behavior after drinking, were also collected.
ResultsThirteen percent of the current participants reported neglecting tooth-brushing after drinking, and their alcohol consumption was higher than those who did not neglect tooth-brushing. Logistic regression analysis showed that high %BOP (%BOP 20) was associated with male (OR=1.53; 95% CI, 1.01-2.33), neglect of tooth-brushing after drinking (OR=2.60; 95% CI, 1.20-5.61) and debris index (OR=8.38; 95% CI, 4.24-16.60) in participants with low alcohol sensitivity. In participants with high alcohol sensitivity, high %BOP was associated with debris index (OR=7.60; 95% CI, 3.12-18.51), but not with any oral health behaviors.
ConclusionsThe study revealed that alcohol consumption was indirectly related to gingival disease activity through the neglect of tooth-brushing after drinking alcohol in university students with low alcohol sensitivity.

DOI： 10.1111/jre.12233

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Relationship between Acute Phase of Chronic Periodontitis and Meteorological Factors in the Maintenance Phase of Periodontal Treatment: A Pilot Study 国際誌

Noriko Takeuchi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH   12 ( 8 )   9119 - 9130   2015年8月

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記述言語：英語   出版者・発行元：MDPI AG  

The acute phase of chronic periodontitis may occur even in patients during supportive periodontal therapy. However, the details are not fully understood. Since the natural environment, including meteorology affects human health, we hypothesized that weather conditions may affect occurrence of acute phase of chronic periodontitis. The aim of this study was to investigate the relationship between weather conditions and acute phase of chronic periodontitis in patients under supportive periodontal therapy. Patients who were diagnosed with acute phase of chronic periodontitis under supportive periodontal therapy during 2011-2013 were selected for this study. We performed oral examinations and collected questionnaires and meteorological data. Of 369 patients who experienced acute phase of chronic periodontitis, 153 had acute phase of chronic periodontitis without direct-triggered episodes. When using the autoregressive integrated moving average model of time-series analysis, the independent covariant of maximum hourly range of barometric pressure, maximum hourly range of temperature, and maximum daily wind speed were significantly associated with occurrence of acute phase of chronic periodontitis (p &lt; 0.05), and 3.1% of the variations in these occurrence over the study period were explained by these factors. Meteorological variables may predict occurrence of acute phase of chronic periodontitis.

DOI： 10.3390/ijerph120809119

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Impact of Oral Commensal Bacteria on Degradation of Periodontal Connective Tissue in Mice 国際誌

Koichiro Irie, Takaaki Tomofuji, Daisuke Ekuni, Manabu Morita, Yoshihiro Shimazaki, Richard P. Darveau

JOURNAL OF PERIODONTOLOGY   86 ( 7 )   899 - 905   2015年7月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Innate and adaptive immunosurveillance mechanisms in response to the normal commensal bacteria can affect periodontal innate defense status. However, it is still unclear how commensal bacteria contribute to the inflammatory responses of junctional epithelium (JE) and periodontal connective tissue (PCT). The aim of the present study is to investigate the contribution of commensal bacteria on inflammatory responses in JE and PCT in mice.
Methods: The periodontal tissue of germ-free (GF) and specific-pathogen-free (SPF) mice were compared at age 11 to 12 weeks (n = 6 per group). In this study, the number of neutrophils and expression of intercellular adhesion molecule (ICAM)-1, fibroblast growth factor receptor (FGFR)-1, matrix metalloproteinase (MMP)-1, and MMP-8 within the JE and the PCT are evaluated. The collagen density was also determined in PCT stained with picrosirius red (PSR). PSR staining combined with or without polarized light microscopy has been used to assess the organization and maturation of collagen matrix.
Results: In the present findings, the area of JE in SPF mice was significantly greater than that in GF mice (P &lt;0.05). In addition, the JE and PCT in SPF mice showed greater migration of neutrophils and higher expression of ICAM-1, FGFR-1, MMP-1, and MMP-8 than those in GF mice (P &lt;0.05). Furthermore, the density of collagen in PCT in SPF mice was lower compared to GF mice (P &lt;0.05).
Conclusion: These results indicate that commensal bacteria induced a low-grade inflammatory state in JE and that such conditions may contribute to degradation of collagen in PCT in mice.

DOI： 10.1902/jop.2015.150006

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Structural equation modeling to assess gender differences in the relationship between psychological symptoms and dental visits after dental check-ups for university students 国際誌

Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

ACTA ODONTOLOGICA SCANDINAVICA   73 ( 5 )   368 - 374   2015年7月

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記述言語：英語   出版者・発行元：INFORMA HEALTHCARE  

Objective. Some studies have shown a relationship between psychological symptoms and oral health behaviors. However, it is unknown whether gender differences affect the relationship between psychological symptoms and oral health behaviors. In addition, gender differences in the relationship between dental anxiety and dental visits for treatment or regular check-up are unclear. The objective of the present study was to explain the relationships among gender differences, psychological symptoms, oral health behaviors, dental anxiety and 'expectation of dental visit', evaluated as 'dental visits when treatments are recommended' in university students. Materials and methods. A total of 607 students (311 males, 296 females) aged 18-38 years old were examined. The information was collected via questionnaire regarding gender, psychological symptoms and oral health behaviors. Psychological symptoms were assessed using the Hopkins Symptom Checklist. Structural equation modeling was used to test pathways from these factors to 'expectation of dental visit'. Multiple-group modeling was also conducted to test for gender differences. Results. Psychological symptoms were related to low expectation of dental visit in females, but there was no such relationship in males. Oral health behaviors were related to expectation of dental visit in both genders. Conclusions. Psychological symptoms were directly related to expectation of dental visit in females and oral health behaviors were related to expectation of dental visit in both genders. To promote dental visits after dental check-ups at school, it might be necessary to improve oral health behaviors in both genders and to evaluate psychological symptoms, especially in females.

DOI： 10.3109/00016357.2014.968870

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Association Between Self-Reported Bruxism and Malocclusion in University Students: A Cross-Sectional Study

Kota Kataoka, Daisuke Ekuni, Shinsuke Mizutani, Takaaki Tomofuji, Tetsuji Azuma, Mayu Yamane, Yuya Kawabata, Yoshiaki Iwasaki, Manabu Morita

JOURNAL OF EPIDEMIOLOGY   25 ( 6 )   423 - 430   2015年6月

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記述言語：英語   出版者・発行元：JAPAN EPIDEMIOLOGICAL ASSOC  

Objectives: Bruxism can result in temporomandibular disorders, oral pain, and tooth wear. However, it is unclear whether bruxism affects malocclusion. The aim of this study was to examine the association between self-reported bruxism and malocclusion in university students.
Methods: Students (n = 1503; 896 men and 607 women) aged 18 and 19 years were examined. Malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need. The presence of buccal mucosa ridging, tooth wear, dental impression on the tongue, palatal/mandibular torus, and the number of teeth present were recorded, as well as body mass index (BMI). Additional information regarding gender, awareness of bruxism, orthodontic treatment, and oral habits was collected via questionnaire.
Results: The proportion of students with malocclusion was 32% (n = 481). The awareness of clenching in males with malocclusion was significantly higher than in those with normal occlusion (chi square test, P &lt; 0.01). According to logistic regression analysis, the probability of malocclusion was significantly associated with awareness of clenching (odds ratio [OR] 2.19; 95% confidence interval [CI], 1.22-3.93) and underweight (BMI &lt; 18.5 kg/m(2)) (OR 1.89; 95% CI, 1.31-2.71) in males but not in females. In subgroup analyses, the probability of crowding was also significantly associated with awareness of clenching and underweight (P &lt; 0.01) in males.
Conclusions: Awareness of clenching and underweight were related to malocclusion (crowding) in university male students.

DOI： 10.2188/jea.JE20140180

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多職種連携の中で専門的口腔衛生管理を行い、経口摂取を継続しながら化学放射線治療が完遂できた一症例

志茂 加代子, 丸山 貴之, 横井 彩, 山中 玲子, 三浦 留美, 江國 大輔, 森田 学

口腔衛生学会雑誌   65 ( 2 )   187 - 187   2015年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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天然植物由来成分配合歯磨剤のラット歯周炎に対する影響

友藤 孝明, 江國 大輔, 東 哲司, 米田 俊樹, 片岡 広太, 川端 勇也, 山根 真由, 森田 学

日本歯周病学会会誌   57 ( 春季特別 )   126 - 126   2015年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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2型糖尿病患者における非外科的歯周病治療の効果の検討

水野 裕文, 江國 大輔, 米田 俊樹, 片岡 広太, 川端 勇也, 水谷 慎介, 友藤 孝明, 森田 学

口腔衛生学会雑誌   65 ( 2 )   186 - 186   2015年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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大学生における第二大臼歯の埋伏が第一大臼歯の歯周状態に与える影響

宮井 久敬, 江國 大輔, 水谷 慎介, 東 哲司, 片岡 広太, 山根 真由, 水野 裕文, 國友 宗義, 谷口 綾乃, 大森 智栄, 森田 学

口腔衛生学会雑誌   65 ( 2 )   180 - 180   2015年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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還元型コエンザイムQ10の抜歯窩創傷治癒への効果の検討

米田 俊樹, 友藤 孝明, 川端 勇也, 江國 大輔, 東 哲司, 片岡 広太, 國友 宗義, 森田 学

口腔衛生学会雑誌   65 ( 3 )   312 - 312   2015年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Relationship between xerostomia and gingival condition in young adults

S. Mizutani, D. Ekuni, T. Tomofuji, T. Azuma, K. Kataoka, M. Yamane, Y. Iwasaki, M. Morita

JOURNAL OF PERIODONTAL RESEARCH   50 ( 1 )   74 - 79   2015年2月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and ObjectiveXerostomia is a subjective symptom of dryness in the mouth. Although a correlation between xerostomia and oral conditions in the elderly has been reported, there are few such studies in the young adults. The aim of this study was to examine the relationship of xerostomia with the gingival condition in university students.
Material and MethodsA total of 2077 students (1202 male subjects and 875 female subjects), 18-24years of age, were examined. The disease activity and severity of the gingival condition were assessed as the percentage of teeth with bleeding on probing (%BOP) and the presence of teeth with probing pocket depth of 4mm, respectively. Additional information on xerostomia, oral health behaviors, coffee/tea intake and nasal congestion was collected via a questionnaire. Path analysis was used to test pathways from xerostomia to the gingival condition.
ResultsOne-hundred and eighty-three (8.8%) students responded that their mouths frequently or always felt dry. Xerostomia was related to %BOP and dental plaque formation, but was not related to the presence of probing pocket depth 4mm. In the structural model, xerostomia was related to dental plaque formation (p&lt;0.01), and a lower level of dental plaque formation was associated with a lower %BOP. Xerostomia was associated with coffee/tea intake (p&lt;0.01) and nasal congestion (p&lt;0.001).
ConclusionXerostomia was indirectly related to gingival disease activity through the accumulation of dental plaque. Nasal congestion and coffee/tea intake also affected xerostomia. These findings suggest that xerostomia should be considered in screening for gingivitis risk in young adults.

DOI： 10.1111/jre.12183

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Relationship between acetaldehyde concentration in mouth air and tongue coating volume 国際誌

Aya Yokoi, Takayuki Maruyama, Reiko Yamanaka, Daisuke Ekuni, Takaaki Tomofuji, Haruhiko Kashiwazaki, Yutaka Yamazaki, Manabu Morita

JOURNAL OF APPLIED ORAL SCIENCE   23 ( 1 )   64 - 70   2015年1月

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記述言語：英語   出版者・発行元：UNIV SAO PAULO FAC ODONTOLOGIA BAURU  

Objective: Acetaldehyde is the first metabolite of ethanol and is produced in the epithelium by mucosal ALDH, while higher levels are derived from microbial oxidation of ethanol by oral microflora such as Candida species. However, it is uncertain whether acetaldehyde concentration in human breath is related to oral condition or local production of acetaldehyde by oral microflora. The aim of this pilot study was to investigate the relationship between physiological acetaldehyde concentration and oral condition in healthy volunteers. Material and Methods: Sixty-five volunteers (51 males and 14 females, aged from 20 to 87 years old) participated in the present study. Acetaldehyde concentration in mouth air was measured using a portable monitor. Oral examination, detection of oral Candida species and assessment of alcohol sensitivity were performed. Results: Acetaldehyde concentration [median (25%, 75%)] in mouth air was 170.7 (73.5, 306.3) ppb. Acetaldehyde concentration in participants with a tongue coating status score of 3 was significantly higher than in those with a score of 1 (p&lt;0.017). After removing tongue coating, acetaldehyde concentration decreased significantly (p&lt;0.05). Acetaldehyde concentration was not correlated with other clinical parameters, presence of Candida species, smoking status or alcohol sensitivity. Conclusion: Physiological acetaldehyde concentration in mouth air was associated with tongue coating volume.

DOI： 10.1590/1678-775720140223

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Self-efficacy and progression of periodontal disease: A prospective cohort study. 国際誌

Azuma T, Yamane M, Ekuni D, Kawabata Y, Kataoka K, Kasuyama K, Maruyama T, Tomofuji T, Morita M

J Clin Periodontol.   42 ( 12 )   1083 - 1089   2015年

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記述言語：英語  

DOI： 10.1111/jcpe.12476

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歯周疾患と糖尿病等との関係に着目した歯科保健指導方法の開発等に関する研究

森田学, 和田淳, 友藤孝明, 江國大輔, 安藤雄一, 荻野景規

厚生労働科学研究費補助金 循環器疾患・糖尿病等生活習慣病対策総合研事業 平成26年度 総括・分担研究報告書   2015年

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大学生における早食いと肥満の関係

山根真由, 江國大輔, 森田 学

厚生の指標   62 ( 7 )   7 - 11   2015年

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記述言語：日本語   出版者・発行元：厚生労働統計協会  

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その他リンク： http://search.jamas.or.jp/link/ui/2015373805

塩化セチルピリジニウム配合トローチ剤の口臭抑制効果

友藤 孝明, 片岡 広太, 川端 勇也, 水谷 慎介, 江國 大輔, 森田 学

日口臭誌   6 ( 1 )   15 - 20   2015年

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記述言語：日本語   出版者・発行元：日本口臭学会  

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その他リンク： http://search.jamas.or.jp/link/ui/2015287943

歯周病メインテナンス期患者に対する血液検査による生活習慣病スクリーニングの検討

町田達哉, 友藤孝明, 江國大輔, 東 哲司, 竹内倫子, 丸山貴之, 川端勇也, 森田 学

口腔衛生会誌   65 ( 4 )   354 - 361   2015年

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記述言語：日本語   出版者・発行元：有限責任中間法人日本口腔衛生学会  

生活習慣病予防はわが国喫緊の課題である.生活習慣病スクリーニングを受ける機会の提供,生活習慣病に関する情報提供,および,医療機関への適切な受診勧奨等が生活習慣病予防のために重要である.本研究では,これらを実施する場の一つとして歯科を捉え,その可能性を検討することを目的とした.簡易な血液検査による生活習慣病スクリーニングを歯周病メインテナンス期患者に行い,分析した.2014年1月から7月に岡山大学病院予防歯科外来を受診した歯周病メインテナンス期患者63名(男性22名,女性41名,平均年齢65.9±6.7歳)を対象に,自己記入式質問紙調査と指尖血の採取を行い,肝疾患,高脂血症(脂質異常症),腎疾患,痛風,糖尿病前症,および糖尿病の疑いの有無を判定した.過去1年間の医療機関受診歴を53名(84.1%)が有していた.過去1年間に肝疾患,高脂血症,腎疾患,痛風,および糖尿病の診断のある者は,それぞれ3名,14名,2名,1名,3名おり,各々の分析から除外された.血液検査の結果,肝疾患,高脂血症,腎疾患,痛風,糖尿病前症,および糖尿病の疑いは,それぞれ対象者の16.7%,34.7%,16.4%,1.6%,46.7%,および6.7%に認められた.医療機関受診歴を有する者の多さに関わらず,生活習慣病予防が必要と思われる者の割合は十分多かった.歯科の場において,簡易な生活習慣病スクリーニングを行い,医療機関への受診勧奨等を行う流れを作ることで,国民の生活習慣病予防の一端を担えるかもしれない.

DOI： 10.5834/jdh.65.4_354

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Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study. 国際誌

Azuma T, Yamane M, Ekuni D, Kawabata Y, Kataoka K, Kasuyama K, Maruyama T, Tomofuji T, Morita M

antioxidants   4 ( 3 )   513 - 22   2015年

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記述言語：英語  

DOI： 10.3390/antiox4030513

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口腔内アセトアルデヒド濃度と舌苔スコアとの関係

横井 彩, 江國大輔, 森田 学

日本歯科評論   75 ( 7 )   139 - 141   2015年

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記述言語：日本語   出版者・発行元：(株)ヒョーロン・パブリッシャーズ  

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Serum Oxidative/anti-oxidative Stress Balance Is Dysregulated in Potentially Pulmonary Hypertensive Patients with Liver Cirrhosis: A Case Control Study

Masako Terao, Akinobu Takaki, Takayuki Maruyama, Hiroki Oe, Tetsuya Yasunaka, Naofumi Tamaki, Kazufumi Nakamura, Takaaki Tomofuji, Takahito Yagi, Hiroshi Sadamori, Yuzo Umeda, Susumu Shinoura, Ryuichi Yoshida, Kazuhiro Nouso, Daisuke Ekuni, Kazuko Koike, Fusao Ikeda, Hidenori Shiraha, Manabu Morita, Hiroshi Ito, Toshiyoshi Fujiwara, Kazuhide Yamamoto

INTERNAL MEDICINE   54 ( 22 )   2815 - 2826   2015年

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記述言語：英語   出版者・発行元：JAPAN SOC INTERNAL MEDICINE  

Objective Hepatopulmonary syndrome (HPS) is characterized by vascular dilatation and hyperdynamic circulation, while portopulmonary hypertension (POPH) is characterized by vasoconstriction with fibrous obliteration of the vascular bed. Vasoactive molecules such as nitric oxide (NO) are candidate factors for cirrhotic complications associated with these diseases. However, oxidative stress balance is not well characterized in HPS and POPH. The present objective is to investigate the oxidative stress and anti-oxidative stress balance and NO pathway balance in patients with potential HPS and POPH.
Methods We recruited patients with decompensated cirrhosis (n = 69) admitted to our hospital as liver transplantation candidates. Patients exhibiting partial pressure of oxygen lower than 80 mmHg and alveolararterial oxygen gradient (AaDO(2)) &gt;= 15 mmHg were categorized as potentially having HPS (23 of 69 patients). Patients exhibiting a tricuspid regurgitation pressure gradient &gt;= 25 mmHg were categorized as potentially having POPH (29 of 61 patients). Serum reactive oxygen metabolites were measured and anti-oxidative OXY-adsorbent test (OXY) were performed, and the balance of these tests was defined as the oxidative index. The correlation between these values and the clinical characteristics of the patients were assessed in a cross-sectional study.
Results Potential HPS patients exhibited no correlation with oxidative stress markers. Potential POPH patients exhibited lower OXY (p = 0.037) and higher oxidative index values (p = 0.001). Additionally, the vascular NO synthase enzyme inhibiting protein, asymmetric dimethylarginine, was higher in potential POPH patients (p = 0.049). The potential POPH patients exhibited elevated AaDO(2), suggesting the presence of pulmonary shunting.
Conclusion Potential POPH patients exhibited elevated oxidative stress with decreased anti-oxidative function accompanied by inhibited NO production. Anti-oxidants represent a candidate treatment for potential POPH patients.

DOI： 10.2169/internalmedicine.54.4889

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歯周組織の加齢変化に対する口腔常在菌の影響

入江浩一郎, 入江浩一郎, 友藤孝明, 江國大輔, 小林周一郎, 菊池毅, 内田瑶子, 福原大樹, 三谷章雄, 嶋崎義浩, 森田学

日本歯周病学会会誌(Web)   57   2015年

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Application of Coenzyme Q10 for Accelerating Soft Tissue Wound Healing after Tooth Extraction in Rats 国際誌

Toshiki Yoneda, Takaaki Tomofuji, Yuya Kawabata, Daisuke Ekuni, Tetsuji Azuma, Kota Kataoka, Muneyoshi Kunitomo, Manabu Morita

NUTRIENTS   6 ( 12 )   5756 - 5769   2014年12月

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記述言語：英語   出版者・発行元：MDPI AG  

Accelerating wound healing after tooth extraction is beneficial in dental treatment. Application of antioxidants, such as reduced coenzyme Q10 (rCoQ10), may promote wound healing after tooth extraction. In this study, we examined the effects of topical application of rCoQ10 on wound healing after tooth extraction in rats. After maxillary first molars were extracted, male Fischer 344 rats (8 weeks old) (n = 27) received topical application of ointment containing 5% rCoQ10 (experimental group) or control ointment (control group) to the sockets for 3 or 8 days (n = 6-7/group). At 3 days after extraction, the experimental group showed higher collagen density and lower numbers of polymorphonuclear leukocytes in the upper part of socket, as compared to the control group (p &lt; 0.05). Gene expression of interleukin-1, tumor necrosis factor-alpha and nuclear factor-kappa B were also lower in the experimental group than in the control group (p &lt; 0.05). At 8 days after tooth extraction, there were no significant differences in collagen density, number of polymorphonuclear leukocytes and bone fill between the groups. Our results suggest that topical application of rCoQ10 promotes wound healing in the soft tissue of the alveolar socket, but that rCoQ10 has a limited effect on bone remodeling in rats.

DOI： 10.3390/nu6125756

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近年における永久歯の萠出年齢の推移

竹内 倫子, 友藤 孝明, 江國 大輔, 山中 玲子, 古田 美智子, 山本 龍生, 恒石 美登里, 森田 学

日本公衆衛生学会総会抄録集   73回   419 - 419   2014年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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Relationships Between Eating Quickly and Weight Gain in Japanese University Students: A Longitudinal Study 国際誌

Mayu Yamane, Daisuke Ekuni, Shinsuke Mizutani, Kota Kataoka, Masami Sakumoto-Kataoka, Yuya Kawabata, Chie Omori, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

OBESITY   22 ( 10 )   2262 - 2266   2014年10月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

ObjectiveMany cross-sectional studies have reported a relationship between overweight/obesity and eating quickly, but there have been few longitudinal studies to address this relationship in younger populations. The purpose of this prospective longitudinal study was to investigate whether eating quickly was related to being overweight in Japanese university students.
MethodsOf 1,396 students who underwent a general examination and completed questionnaires at the start of university and before graduation, 1,314 students (676 male and 638 female) of normal body composition [body mass index (BMI)&lt;25 kg m(-2)] at baseline were included in the analysis. The questionnaires included speed of eating and other lifestyle factors. After a 3-year follow-up, the students whose BMIs were25 kg m(-2) were defined as overweight.
ResultsIn this study, 38 participants (2.9%) became overweight. In the logistic regression analysis, the risk of being overweight was increased in males [adjusted odds ratio (OR): 2.77; 95% confidence interval (CI): 1.33-5.79; P&lt;0.01] and in those who ate quickly at baseline (OR: 4.40; 95% CI: 2.22-8.75; P&lt;0.001).
ConclusionsEating quickly may predict risk of being overweight in Japanese university students.

DOI： 10.1002/oby.20842

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Severe Periodontitis Is Inversely Associated with Coffee Consumption in the Maintenance Phase of Periodontal Treatment 国際誌

Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Noriko Takeuchi, Takayuki Maruyama, Shinsuke Mizutani, Kota Kataoka, Yuya Kawabata, Manabu Morita

NUTRIENTS   6 ( 10 )   4476 - 4490   2014年10月

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記述言語：英語   出版者・発行元：MDPI AG  

This cross-sectional study addressed the relationship between coffee consumption and periodontitis in patients during the maintenance phase of periodontal treatment. A total of 414 periodontitis patients in the maintenance phase of periodontal treatment completed a questionnaire including items related to coffee intake and underwent periodontal examination. Logistic regression analysis showed that presence of moderate/severe periodontitis was correlated with presence of hypertension (Odds Ratio (OR) = 1.99, p &lt; 0.05), smoking (former, OR = 5.63, p &lt; 0.01; current, OR = 6.81, p = 0.076), number of teeth present (OR = 0.89, p &lt; 0.001), plaque control record &gt;= 20% (OR = 1.88, p &lt; 0.05), and duration of maintenance phase (OR = 1.07, p &lt; 0.01). On the other hand, presence of severe periodontitis was correlated with smoking (former, OR = 1.35, p = 0.501; current, OR = 3.98, p &lt; 0.05), coffee consumption (&gt;= 1 cup/day, OR = 0.55, p &lt; 0.05), number of teeth present (OR = 0.95, p &lt; 0.05), and bleeding on probing &gt;= 20% (OR = 3.67, p &lt; 0.001). There appears to be an inverse association between coffee consumption (&gt;= 1 cup/day) and prevalence of severe periodontitis in the maintenance phase of periodontal treatment.

DOI： 10.3390/nu6104476

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若年者の早食いは肥満のリスクになる

山根 真由, 江國 大輔, 友藤 孝明, 森田 学

日本公衆衛生学会総会抄録集   73回   209 - 209   2014年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats 国際誌

Daisuke Ekuni, Toshiki Yoneda, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Shinsuke Mizutani, Tetsuji Azuma, Takaaki Tomofuji, Manabu Morita

LIPIDS IN HEALTH AND DISEASE   13 ( 1 )   144 - 144   2014年9月

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記述言語：英語   出版者・発行元：BIOMED CENTRAL LTD  

Background: Psychosocial stress is one of the risk factors for atherosclerosis. As occlusal disharmony induces psychological stress, we hypothesized that psychological stress by occlusal disharmony accelerates atherosclerosis. The aim of this study was to investigate the effects of occlusal disharmony on the initiation of atherosclerosis in apolipoprotein E (apoE) knockout rats.
Methods: Fourteen male apoE-knockout rats (age; 8 weeks) (Sprague-Dawley strain background) were divided into two groups of seven rats: the occlusal disharmony group and the no treatment (control) group. In the occlusal disharmony group, the maxillary molar cusps were cut off for the 8-week experimental period.
Results: In the occlusal disharmony group, the percentages of the area of total aortic lumen occupied by plaques and lipid were significantly higher than those in the control group (p &lt; 0.05, t-test). The occlusal disharmony group also showed significantly higher serum levels of very low-density lipoprotein-cholesterol (VLDL) and low-density lipoprotein-cholesterol (LDL), plasma levels of corticosterone (1.9, 1.3 and 1.3 times, respectively), higher aortic protein expression levels of vascular cell adhesion molecule-1 (VCAM1) and intercellular adhesion molecule-1 (ICAM1) (1.5 and 1.4 times, respectively), and higher aortic gene expression of levels of VCAM1 and Toll-like receptor 4 (TLR4) (1.9 and 4.3 times, respectively), as compared to the control group (p &lt; 0.05). However, there were no significant differences in serum levels of oxidized LDL, reactive oxygen metabolites and C-reactive protein between the two groups.
Conclusion: In apoE knockout rats, occlusal disharmony may induce VCAM1, ICAM1 and TLR4 expression and accelerate the initiation of atherosclerosis.

DOI： 10.1186/1476-511X-13-144

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分子イメージングの可能性 歯学分野での応用と今後の展望

江國 大輔

Journal of Oral Biosciences Supplement   2014   104 - 104   2014年9月

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記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

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Relationship between increases in BMI and changes in periodontal status: a prospective cohort study 国際誌

Daisuke Ekuni, Shinsuke Mizutani, Azusa Kojima, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Toshiki Yoneda, Michiko Furuta, Nobuoki Eshima, Yoshiaki Iwasaki, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY   41 ( 8 )   772 - 778   2014年8月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Aim: The purpose of this prospective cohort study was to investigate whether body mass index (BMI) and oral health behaviour are related to changes in periodontal status in Japanese university students.
Materials and Methods: Students (n = 224) who were interested in receiving oral health examinations before entering university and before graduation were included in the analysis. Subjects were investigated regarding the correlations of oral health behaviours and increases in BMI with the percentage of bleeding on probing (% BOP) and Community Periodontal Index (CPI) scores as indicators of changes in periodontal status.
Results: The risk of increased % BOP was associated with the non-use of dental floss (adjusted odds ratio [OR]: 3.11; 95% confidence interval [CI]: 1.31-7.37; p &lt; 0.05), whereas the risk of increased CPI score was associated with increases in BMI (OR: 1.95; 95% CI: 1.05-3.65; p &lt; 0.05) and simplified oral hygiene index score (OR: 2.28; 95% CI: 1.23-4.22; p &lt; 0.01).
Conclusion: Increases in BMI were associated with worsening of periodontal status, defined as increased CPI score in Japanese university students, whereas lack of inter-dental cleaning was associated with exacerbated gingival bleeding.

DOI： 10.1111/jcpe.12273

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Effects of hydrogen-rich water on aging periodontal tissues in rats 国際誌

Takaaki Tomofuji, Yuya Kawabata, Kenta Kasuyama, Yasumasa Endo, Toshiki Yoneda, Mayu Yamane, Tetsuji Azuma, Daisuke Ekuni, Manabu Morita

SCIENTIFIC REPORTS   4   5534 - 5534   2014年7月

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記述言語：英語   出版者・発行元：NATURE PUBLISHING GROUP  

Oxidative damage is involved in age-related inflammatory reactions. The anti-oxidative effects of hydrogen-rich water suppress oxidative damage, which may aid in inhibiting age-related inflammatory reactions. We investigated the effects of drinking hydrogen-rich water on aging periodontal tissues in healthy rats. Four-month-old male Fischer 344 rats (n = 12) were divided into two groups: the experimental group (hydrogen-rich water treatment) and the control group (distilled water treatment). The rats consumed hydrogen-rich water or distilled water until 16 months of age. The experimental group exhibited lower periodontal oxidative damage at 16 months of age than the control group. Although protein expression of interleukin-1 beta did not differ, gene expression of Nod-like receptor protein 3 inflammasomes was activated in periodontal tissues from the experimental group as compared with the control group. Drinking hydrogen-rich water is proposed to have anti-aging effects on periodontal oxidative damage, but not on inflammatory reactions in healthy rats.

DOI： 10.1038/srep05534

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若年者における急性上気道炎の発症頻度と智歯萠出状態との関連

町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 東 哲司, 友藤 孝明, 江國 大輔, 森田 学

口腔衛生学会雑誌   64 ( 4 )   328 - 335   2014年7月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

口腔状態と気道感染の関連性の議論が近年盛んである。口腔は上気道と接しており、半萠出智歯の存在は、隣在歯との間に清掃困難な空間を形成し、智歯周囲の細菌量を増加させ、上気道を含む周囲組織に影響を与えうる。そこで、対象者を智歯萠出時期である18-20歳の大学生とし、急性上気道炎の発症頻度と智歯萠出状態の関連を検討することとした。2013年岡山大学新入生健康診断の際に歯科健診を任意受診した18-67歳(平均年齢18.5±2.1歳)の2,205名中、非喫煙者でデータ欠損のない18-20歳の1,964名(男性1,130名、女性834名)を分析対象とした。口腔診査を行い口腔清掃度、地域歯周疾患指数、および智歯萠出状態を調べた。自己記入式質問調査により過去1年間の急性上気道炎の発症頻度を尋ね、年間3回以上発症した群と3回未満発症した群の2群に分けた。χ2検定で2群比較を行い、有意な関連を智歯萠出状態と過去1年間の一番奥の歯ぐきの痛みの既往で認めた(P&lt;0.05)。口腔清掃度にも一定の関連性を認めた(P=0.054)。性別、口腔清掃度、智歯萠出状態、過去1年間の一番奥の歯ぐきの痛みの既往を説明変数としたロジスティック回帰分析を行ったところ、萠出智歯なしの者に対し、半萠出智歯を有する者は急性上気道炎を過去1年間に3回以上発症していた者の割合が有意に高かった(P=0.003)。大学生において智歯萠出状態と急性上気道炎の発症頻度に関連性があることが示唆された。(著者抄録)

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その他リンク： http://search.jamas.or.jp/link/ui/2014337245

某事業所の歯科健診受診者における歯周病の知識がない者の特徴

山本 龍生, 渕田 慎也, 平田 幸夫, 江國 大輔, 森田 学, 恒石 美登里, 鈴木 洋美, 島村 広海, 安信 忠義, 公衆歯科衛生研究会

産業衛生学雑誌   56 ( 臨増 )   428 - 428   2014年5月

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記述言語：日本語   出版者・発行元：(公社)日本産業衛生学会  

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歯周状態と全身性の酸化ストレスとの縦断的な関連

森田 学, 町田 達哉, 友藤 孝明, 江國 大輔, 山根 真由, 米田 俊樹, 川端 勇也, 片岡 広太

産業衛生学雑誌   56 ( 臨増 )   429 - 429   2014年5月

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記述言語：日本語   出版者・発行元：(公社)日本産業衛生学会  

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職域成人における口腔内所見および口腔保健行動とメタボリックシンドロームとの関係

林 浩範, 江國 大輔, 米田 俊樹, 水谷 慎介, 赤澤 百合子, 森田 学

産業衛生学雑誌   56 ( 臨増 )   429 - 429   2014年5月

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記述言語：日本語   出版者・発行元：(公社)日本産業衛生学会  

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若年者における血圧と歯周炎との関連についてのコホート研究

川端 勇也, 江國 大輔, 友藤 孝明, 東 哲司, 丸山 貴之, 水谷 慎介, 米田 俊樹, 町田 達哉, 片岡 広太, 山根 真由, 佐久本 雅美, 大森 智栄, 森田 学

口腔衛生学会雑誌   64 ( 2 )   175 - 175   2014年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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口腔常在菌叢による歯周組織への影響

入江 浩一郎, ダービュ・リチャード, 友藤 孝明, 江國 大輔, 森田 学, 嶋崎 義浩

日本歯周病学会会誌   56 ( 春季特別 )   120 - 120   2014年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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肥満と歯周病進行との関連

米田 俊樹, 江國 大輔, 水谷 慎介, 児島 梓, 友藤 孝明, 入江 浩一郎, 東 哲司, 森田 学

口腔衛生学会雑誌   64 ( 3 )   316 - 316   2014年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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大学生における第二大臼歯の埋伏状況と口腔内の他の因子との関係

大森 智栄, 江國 大輔, 片岡 広太, 山根 真由, 水谷 慎介, 東 哲司, 友藤 孝明, 森田 学

口腔衛生学会雑誌   64 ( 3 )   315 - 315   2014年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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若年者における口腔乾燥感と歯周病との関連

水谷 慎介, 江國 大輔, 友藤 孝明, 東 哲司, 片岡 広太, 山根 真由, 森田 学

口腔衛生学会雑誌   64 ( 3 )   312 - 312   2014年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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咬合異常によるストレスはアポリポタンパクE欠損ラットの動脈硬化を増悪する

江國 大輔, 米田 俊樹, 遠藤 康正, 粕山 健太, 入江 浩一郎, 水谷 慎介, 東 哲司, 友藤 孝明, 森田 学

口腔衛生学会雑誌   64 ( 2 )   230 - 230   2014年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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口腔常在菌に対する宿主防御機構への影響

入江 浩一郎, 友藤 孝明, 江國 大輔, 森田 学, 嶋崎 義浩

口腔衛生学会雑誌   64 ( 2 )   181 - 181   2014年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯周病による全身の酸化ストレスへの影響 酸化ストレス可視化マウスを用いた検討

片岡 広太, 江國 大輔, 粕山 健太, 川端 勇也, 山根 真由, 遠藤 康正, 米田 俊樹, 友藤 孝明, 森田 学

口腔衛生学会雑誌   64 ( 2 )   181 - 181   2014年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Anti-ageing effects of dentifrices containing anti-oxidative, anti-inflammatory, and anti-bacterial agents (Tomarina®) on gingival collagen degradation in rats.

Koichiro I, Tomofuji T, Ekuni D, Endo Y, Kasuyama K, Azuma T, Tamaki N, Yoneda T, Morita M

Arch Oral Biol   59 ( 1 )   60 - 65   2014年1月

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記述言語：英語  

DOI： 10.1016/j.archoralbio.2013.09.008

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Factors related to the formation of buccal mucosa ridging in university students 国際誌

Shinsuke Mizutani, Daisuke Ekuni, Takaaki Tomofuji, Tetsuji Azuma, Koichiro Irie, Tatsuya Machida, Toshiki Yoneda, Yoshiaki Iwasaki, Manabu Morita

ACTA ODONTOLOGICA SCANDINAVICA   72 ( 1 )   58 - 63   2014年1月

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記述言語：英語   出版者・発行元：TAYLOR & FRANCIS LTD  

Objective. Buccal mucosa ridging (BMR) is known as a clinical sign of clenching, which is one of the major manifestations of bruxism. However, there are few reports about the formation of BMR and no data regarding the association between BMR and factors such as malocclusion. The purpose of the current study was to investigate the relationship between BMR and factors such as the number of teeth present, gender, body mass index (BMI), occlusion and clenching habit in university students. Materials and methods. A total of 2101 students (1164 males, 937 females), aged 18-29 years old, were included in the study. BMR and the number of teeth present were recorded and malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need. Additional information regarding gender, clenching habit and BMI was collected via a questionnaire. Results. Forty-six per cent of the subjects had BMR and the prevalence of BMR in females was significantly higher than that of males (chi square test, p &lt; 0.001). According to logistic regression analysis, the probability of BMR was significantly associated with female gender (OR = 1.501, 95% CI = 1.259-1.790, p &lt; 0.001), crowding (OR = 2.102, 95% CI = 1.706-2.590, p &lt; 0.001) and overjet (OR = 0.585, 95% CI = 0.418-0.818, p = 0.002). On the other hand, BMR was not associated with awareness of clenching habit and BMI. Conclusions. Gender, crowding and overjet were related to the formation of BMR in university students. When evaluating BMR as a clinical sign of clenching, one might have to take factors such as gender and crowding into consideration.

DOI： 10.3109/00016357.2013.797102

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Periodontitis and Atherosclerosis.

Ekuni D, Tomofuji T, Morita M

Studies on Periodontal Disease   183 - 195   2014年

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Effects of Antioxidants on Periodontal Disease.

Tomofuji T, Ekuni D, Mizutani S, Morita M

Studies on Periodontal Disease   279 - 305   2014年

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Periodontitis and Liver Diseases.

Irie K, Ekuni D, Tomofuji T, Morita M

Studies on Periodontal Disease   197 - 209   2014年

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Association between oxidative stress and periodontal diseases in animal model studies

Morita M, Ekuni D, Tomofuji T

Springer   2014年

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Longitudinal Relationship between Plasma Reactive Oxygen Metabolites and Periodontal Condition in the Maintenance Phase of Periodontal Treatment

Tatsuya Machida, Takaaki Tomofuji, Daisuke Ekuni, Mayu Yamane, Toshiki Yoneda, Yuya Kawabata, Kota Kataoka, Naofumi Tamaki, Manabu Morit

DISEASE MARKERS   2014   489292 - 489292   2014年

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記述言語：英語   出版者・発行元：HINDAWI PUBLISHING CORPORATION  

Aim. The present cohort study describes the longitudinal relationship between plasma oxidative status and periodontitis progression during the maintenance phase of treatment. Materials and Methods. Forty-five patients (mean age 58.8 years) were monitored from 2008 to 2013. Periodontal conditions, including probing pocket depth (PPD) and clinical attachment level (CAL), were recorded. Measurements of plasma reactive oxygen metabolites (ROM) and biologic antioxidant potential (BAP) were performed to evaluate plasma oxidative status. The patients were assigned into 2 groups as low and high plasma ROM level using a cut-off value which was median of plasma ROM level at baseline. Results. In the subjects with low plasma ROM level at baseline, changes in mean CAL were positively correlated with changes in plasma ROM levels, bleeding on probing, and plaque control record, but not with PPD. In the subjects with high plasma ROM at baseline, changes in CAL were significantly associated with only PPD at baseline. On the other hands there were no significant associations between changes in CAL and those in plasma BAP levels. Conclusions. When plasma ROM level in periodontitis patients was low, increases in plasma ROM level were associated with those in CAL during the maintenance phase of treatment.

DOI： 10.1155/2014/489292

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Association Between Oxidative Stress and Periodontal Diseases in Animal Model Studies.

Morita M, Ekuni D, Tomofuji T

Studies on Periodontal Disease   33 - 51   2014年

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Effects of antioxidants on periodontal disease.

Tomofuji T, Ekuni D, Mizutani S, Morita M

Springer   2014年

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Effects of Periodontal Therapy on Circulating Oxidative Stress.

Tomofuji T, Ekuni D, Morita M

Studies on Periodontal Disease   147 - 156   2014年

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Expression of Reactive Oxygen Species in Junctional and Pocket Epithelium.

Ekuni D, Firth JD, Putnins EE

Studies on Periodontal Disease   53 - 75   2014年

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Effects of periodontal therapy on circulating oxidative stress.

Tomofuji T, Ekuni D, Morita M

Springer   2014年

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Expression of reactive oxygen species in junctional and pocket epithelium.

ekuni D, Firth JD, Putnins EE

Springer   2014年

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Periodontitis and liver diseases.

Irie K, Ekuni D, Tomofuji T, Morita M

Springer   2014年

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Periodontitis and atherosclerosis.

Ekuni D, Tomofuji T, Morita M

Springer   2014年

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アルコール摂取後の口腔保健行動の変化と歯周病との関連

山根 真由, 江國 大輔, 水谷 慎介, 町田 達哉, 片岡 広大, 丸山 貴之, 森田 学

岡山歯学会雑誌   32 ( 2 )   87 - 88   2013年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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若年者における風邪の発症頻度と智歯の萠出状態との関連

町田 達哉, 水谷 慎介, 片岡 広太, 山根 真由, 丸山 貴之, 友藤 孝明, 江國 大輔, 森田 学

岡山歯学会雑誌   32 ( 2 )   87 - 87   2013年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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咬合異常はラット海馬のベータアミロイドを誘導する

江國 大輔

岡山歯学会雑誌   32 ( 2 )   77 - 78   2013年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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Relationships between self-rated oral health, subjective symptoms, oral health behavior and clinical conditions in Japanese university students: a cross-sectional survey at Okayama University 国際誌

Azusa Kojima, Daisuke Ekuni, Shinsuke Mizutani, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Yoshiaki Iwasaki, Manabu Morita

BMC ORAL HEALTH   13 ( 1 )   62 - 62   2013年11月

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記述言語：英語   出版者・発行元：BIOMED CENTRAL LTD  

Background: Self-rated oral health is a valid and useful summary indicator of overall oral health status and quality of life. However, few studies on perception of oral health have been conducted among Japanese young adults. This study investigated whether oral health behavior, subjective oral symptoms, or clinical oral status were associated with self-rated oral health in Japanese young adults.
Methods: This cross-sectional survey included 2,087 students (1,183 males, 904 females), aged 18 and 19 years, at Okayama University, Japan. A self-administered questionnaire was distributed and an oral examination was performed.
Results: In a structural equation modeling analysis, the score of decayed, missing and filled teeth (DMFT) significantly affected self-rated oral health (p &lt;0.05) and the effect size was highest. Malocclusion, subjective symptoms of temporomandibular disorders (TMD) and stomatitis, and poor oral health behavior significantly induced self-rated poor oral health with small effect sizes (p &lt;0.05). Clinical periodontal conditions and Oral Hygiene Index-simplified were not related to self-rated oral health.
Conclusion: Self-rated oral health was influenced by subjective symptoms of TMD and stomatitis, oral health behavior, the score of DMFT, and malocclusion. The evaluation of these parameters may be a useful approach in routine dental examination to improve self-rated oral health in university students.

DOI： 10.1186/1472-6831-13-62

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Effects of apoE Deficiency and Occlusal Disharmony on Amyloid-Beta Production and Spatial Memory in Rats 国際誌

Daisuke Ekuni, Yasumasa Endo, Takaaki Tomofuji, Tetsuji Azuma, Koichiro Irie, Kenta Kasuyama, Manabu Morita

PLoS ONE   8 ( 9 )   e74966   2013年9月

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記述言語：英語  

Amyloid-β (Aβ) plays a causative role in Alzheimer's disease. Apolipoprotein E (apoE) is involved in Aβ accumulation, whereas occlusal disharmony increases Aβ production in the rat hippocampus. The purpose of the present study was to investigate the effects of apoE deficiency and occlusal disharmony on Aβ production and spatial memory. Wild-type (WT) (n = 12) and apoE-deficient [ApoE(-/-)] (n = 12) rats (Sprague-Dawley
8 weeks old) were used. These rats were randomly divided into four groups of six rats each: two control (C) groups: WT (C-WT) and ApoE [C-ApoE(-/-)], and two occlusal disharmony (D) groups: WT (D-WT) and ApoE [D-ApoE(-/-)]. The C group received no treatment for 8 weeks. In the D group, the maxillary molar cusps were cut off for 8 weeks. The spatial memory of rats was assessed according to their behavioral performance in a radial arm maze. In both genotypes of rats, significant differences in the reference memory, Aβ42 production, β-secretase expression and plasma corticosterone levels were observed between the C and D groups (P &lt
0.0125). The levels of Aβ42 and glucocorticoid receptor in the C-ApoE(-/-) group were also significantly higher than those in the C-WT group (P &lt
0.0125). However, no significant differences in these parameters were found between the two genotypes with occlusal disharmony. In conclusion, occlusal disharmony induces cognitive dysfunction and Aβ accumulation in the rat hippocampus, and the effects of occlusal disharmony on Aβ accumulation and cognitive dysfunction were larger than those of apoE deficiency. © 2013 Ekuni et al.

DOI： 10.1371/journal.pone.0074966

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還元型コエンザイムQ10の歯周組織の加齢変化に対する効果について

米田 俊樹, 友藤 孝明, 江國 大輔, 東 哲司, 遠藤 康正, 粕山 健太, 町田 達哉, 森田 学

Journal of Oral Biosciences Supplement   2013   170 - 170   2013年9月

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記述言語：日本語   出版者・発行元：(一社)歯科基礎医学会  

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Anti-aging Effects of Co-enzyme Q10 on Periodontal Tissues

T. Yoneda, T. Tomofuji, D. Ekuni, T. Azuma, Y. Endo, K. Kasuyama, T. Machida, M. Morita

JOURNAL OF DENTAL RESEARCH   92 ( 8 )   735 - 739   2013年8月

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記述言語：英語   出版者・発行元：SAGE PUBLICATIONS INC  

Oxidative stress is associated with age-related reactions. The anti-oxidative effects of a reduced form of co-enzyme Q10 (rCoQ10) suppress oxidative stress, which may contribute to the prevention of age-related inflammatory reactions. We examined the effects of topically applied rCoQ10 on periodontal inflammatory reactions in a rat aging model. Male Fischer 344 rats, 2 (n = 6) and 4 mos (n = 18) of age, were used. All of the two-month-old rats and 6 of the four-month-old rats were sacrificed and 12 remaining four-month-old rats received topically applied ointment with or without 1% rCoQ10 on the gingival surface until they reached 6 mos of age. The rats showed an age-dependent increase in circulating oxidative stress. RCoQ10 decreased oxidative DNA damage and tartrate-resistant acid-phosphatase-positive osteoclasts in the periodontal tissue at 6 mos of age as compared with the control. The same conditions lowered gene expression of caspase-1 and interleukin-1 in the periodontal tissue. Furthermore, Nod-like receptor protein 3 inflammasomes were less activated in periodontal tissues from rCoQ10-treated rats as compared with the control rats. Our results suggest that rCoQ10 suppresses age-related inflammatory reactions and osteoclast differentiation by inhibiting oxidative stress.

DOI： 10.1177/0022034513490959

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Effects of eating behaviors on being overweight in Japanese university students: A cross-sectional survey at the Okayama University 国際誌

Daisuke Ekuni, Michiko Furuta, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

Asia-Pacific Journal of Public Health   25 ( 4 )   326 - 334   2013年7月

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記述言語：英語  

Being overweight is an important risk factor for lifestyle-related diseases. The objective of the present study was to examine whether eating until full, eating quickly, or both eating behaviors were associated with being overweight, defined as a body mass index (BMI) ≥ 25, in young adults. This cross-sectional survey comprised 1918 students (1139 male and 779 female students) at the Okayama University in Japan. In logistic regression analysis, eating until full was not associated with being overweight in either sex. The adjusted odds ratio of being overweight among those who reported eating quickly was 3.93 (2.45-6.31
P &lt
.0001) for male and 1.59 (0.79-3.21
P =.193) for female students. Moreover, the adjusted odds ratio of being overweight was 2.72 (1.72-4.30
P &lt
.001) in male students who frequently reported eating a fatty diet. The combination of eating quickly and frequently eating a fatty diet had a supra-additive effect on being overweight in the male students. © 2011 APJPH.

DOI： 10.1177/1010539511425702

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Relationship between masticatory performance and heart rate variability: A pilot study 国際誌

Noriko Takeuchi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

ACTA ODONTOLOGICA SCANDINAVICA   71 ( 3-4 )   807 - 812   2013年5月

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記述言語：英語   出版者・発行元：TAYLOR & FRANCIS LTD  

Objective. Little information is available on how impaired masticatory performance relates to heart-rate variability (HRV). The aim of this study was to investigate the relationship between HRV indices and masticatory performance. Materials and methods. Sixty-five subjects (19 men and 46 women, 66.1 +/- 9.9 years old) who had received periodontal maintenance care at the Clinic of Preventive Dentistry of Okayama University Hospital were selected for the study. All subjects completed written questionnaires, a chewing (colour-changing gum) test for masticatory performance and measurement of occlusal force and HRV as well as oral examination. Results. The high sympathetic activity (LF &gt;= 49.6) group showed a significantly lower level of masticatory performance (a(star) value) than the low sympathetic activity group (p &lt; 0.05). There were also significant correlations of masticatory performance with LF and LF/HF (p &lt; 0.05). Conclusion. These findings suggest that there is relationship between masticatory performance and HRV indices and impaired masticatory performance may be a risk factor for inducing high sympathetic activity.

DOI： 10.3109/00016357.2012.734403

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Relationship between periodontal inflammation and fetal growth in pregnant women: a cross-sectional study 国際誌

Noriko Takeuchi, Daisuke Ekuni, Koichiro Irie, Michiko Furuta, Takaaki Tomofuji, Manabu Morita, Tatsuo Watanabe

ARCHIVES OF GYNECOLOGY AND OBSTETRICS   287 ( 5 )   951 - 957   2013年5月

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記述言語：英語   出版者・発行元：SPRINGER HEIDELBERG  

Periodontal disease in pregnant women, evaluated by probing pocket depth (PPD) or clinical attachment level (CAL), significantly increases the risk of subsequent preterm birth (PB) and/or low birth weight (LBW). However, PPD and CAL do not always reflect current periodontal tissue inflammation. Therefore, assessment of bleeding on probing index (BOP), an indicator of periodontal inflammation, may be appropriate. The objective of this cross-sectional study was to investigate the relationship between periodontal inflammation and fetal growth in pregnant women after periodontal treatment.
We recruited 203 pregnant women (mean age 31.8 +/- A 4.5 years), including 20 subjects with periodontal inflammation and 183 periodontally healthy controls, after periodontal treatment. Fetal growth parameters as well as periodontal conditions were recorded.
Perinatal gestational age was 39.4 +/- A 1.3 weeks; 8 subjects had a PB and 12 had an infant with a LBW. Results of multiple stepwise regression analysis indicated that birth length was negatively correlated with BOP% (beta = -0.175; p = 0.002). Fetal femur length, and birth length and weight among mothers with low periodontal inflammation were significantly higher than those among mothers with high periodontal inflammation (p &lt; 0.05). The LBW rate in the high periodontal inflammation group was significantly higher than that in the low periodontal inflammation group (p &lt; 0.05).
These results suggest that periodontal inflammation is correlated with fetal femur length, birth weight, and birth length.

DOI： 10.1007/s00404-012-2660-4

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P-22 岡山大学病院口臭外来における6年間の患者実態調査(ポスター)

貞森 なみ子, 竹内 倫子, 江國 大輔, 森田 学

口腔衛生学会雑誌   63 ( 2 )   176 - 176   2013年4月

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記述言語：日本語   出版者・発行元：有限責任中間法人日本口腔衛生学会  

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P-36 アポリポ蛋白E欠損と咬合異常によるラット海馬および認知障害への影響(ポスター)

江國 大輔, 遠藤 康正, 友藤 孝明, 東 哲司, 入江 浩一郎, 粕山 健太, 米田 俊樹, 森田 学

口腔衛生学会雑誌   63 ( 2 )   183 - 183   2013年4月

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記述言語：日本語   出版者・発行元：有限責任中間法人日本口腔衛生学会  

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P-56 歯周治療のメインテナンス期における歯周病の進行とコーヒー摂取頻度との関連(ポスター)

東 哲司, 友藤 孝明, 江國 大輔, 入江 浩一郎, 遠藤 康正, 粕山 健太, 水谷 慎介, 町田 達哉, 森田 学

口腔衛生学会雑誌   63 ( 2 )   193 - 193   2013年4月

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記述言語：日本語   出版者・発行元：有限責任中間法人日本口腔衛生学会  

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Effects of electrical stimulation on periodontal tissue remodeling in rats

T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, K. Kasuyama, M. Nagayama, M. Morita

JOURNAL OF PERIODONTAL RESEARCH   48 ( 2 )   177 - 183   2013年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and Objective Electric current is used to promote wound healing. However, it is unclear whether electrical stimulation contributes to gingival tissue remodeling. This study examined the effects of electrical stimulation on gingival tissue remodeling in a rat periodontitis model. Material and Methods Male Wistar rats (n=28, 8wks of age) were divided into four groups of seven rats each. The control group did not receive any treatment for 6wks. In the other groups, periodontitis was ligature-induced for 4wks. After 4wks, the rats with periodontitis were given daily electrical stimulation of 0, 50 or 100A for 2wks. Results The periodontitis group stimulated with 0 A showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue compared with the control group (p&lt;0.05). The two remaining groups treated with 50or 100A of electrical stimulation exhibited a lower density of polymorphonuclear leukocytes (p&lt;0.05) and a higher density of collagen than the group stimulated with 0A (p&lt;0.05). They also showed higher expression of fibroblast growth factor-2 than the group treated with 0A of electrical stimulation (p&lt;0.05). Conclusion Electric stimulation may offer a novel approach to promote gingival tissue remodeling in periodontal lesions.

DOI： 10.1111/j.1600-0765.2012.01518.x

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大学生における頬粘膜圧痕の形成にかかわる因子

水谷 慎介, 江國 大輔, 友藤 孝明, 入江 浩一郎, 東 哲司, 森田 学

口腔衛生学会雑誌   63 ( 3 )   291 - 291   2013年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Lipopolysaccharide induces a stromal-epithelial signalling axis in a rat model of chronic periodontitis 国際誌

James D. Firth, Daisuke Ekuni, Koichiro Irie, Takaaki Tomofuji, Manabu Morita, Edward E. Putnins

JOURNAL OF CLINICAL PERIODONTOLOGY   40 ( 1 )   8 - 17   2013年1月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Aim Lipopolysaccharide is a bacterial virulence factor implicated in chronic periodontitis, which may penetrate the junctional epithelial barrier and basement membrane to insult underlying stroma. We sought to identify lipopolysaccharide-induced global gene expression changes responsible for signalling between stroma and epithelium during disease onset.
Materials and Methods Using a rat lipopolysaccharide periodontitis model, junctional epithelium and underlying stromal tissue were separately collected from healthy and diseased animals by laser-capture microdissection and subject to gene expression microarray analysis. Key gene products identified were validated in gingival epithelial and fibroblast cell cultures.
Results Global gene expression patterns distinguishing health versus disease were found in and between both tissue types. In stroma, the most significantly altered gene ontology function group (Z &gt;= 4.00) was cytokines, containing most significantly (+/- 2-fold; p &lt; 0.05) upregulated genes amphiregulin, IL1-beta and Fas ligand, all positive, diffusible modulators of the epithelial growth factor receptor pathway. In epithelium, the most significant changes were in downregulated FOS-related antigen-1 gene, somatostatin receptor-2 gene and mucin-4 gene, all negative modulators of the epithelial growth factor receptor pathway.
Conclusion These results establish a periodontitis model for studying gene product interactions and suggests that the onset of junctional epithelial disease hyperproliferation involves a concerted stromalepithelial signalling axis.

DOI： 10.1111/jcpe.12023

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Preventive effects of trehalose on osteoclast differentiation in rat periodontitis model 国際誌

Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Kenta Kasuyama, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY   40 ( 1 )   33 - 40   2013年1月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Aim Trehalose, which is a disaccharide formed by a 1,1 linkage of two glucose molecules, was suggested to have a suppressive effect on bone resorption. In this study, we examined the effects of topical application of trehalose on osteoclast differentiation in a rat periodontitis model.
Material and Methods Rats were divided into four groups. One group received no treatment. In the other groups, experimental periodontitis was induced by ligature placement. These rats with experimental periodontitis received topical application of pure water (vehicle group), 30 mg/ml trehalose solution (30 mg/ml trehalose group) or 60 mg/ml trehalose solution (60 mg/ml trehalose group) to the gingival sulcus respectively.
Results The vehicle group showed higher numbers of polymorphonuclear leucocytes, receptor activator of nuclear factor kappa B ligand (RANKL)-positive cells and osteoclasts compared with the no treatment group respectively. Trehalose-applied groups exhibited lower numbers of these cells compared with the vehicle group. Gene expressions of tumour necrosis factor-a, RANKL and toll-like receptor 4 were suppressed by trehalose. In addition, protein expressions of RANKL inducing pathway were less activated by trehalose.
Conclusion Topical application of trehalose could suppress osteoclast differentiation by inactivation of RANKL inducing pathway in the rat periodontitis model.

DOI： 10.1111/jcpe.12032

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Involvement of toll-like receptor 2 and 4 in association between dyslipidemia and osteoclast differentiation in apolipoprotein E deficient rat periodontium 国際誌

Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Kenta Kasuyama, Toshiki Yoneda, Manabu Morita

LIPIDS IN HEALTH AND DISEASE   12 ( 1 )   1 - 1   2013年1月

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記述言語：英語   出版者・発行元：BIOMED CENTRAL LTD  

Background: Dyslipidemia increases circulating levels of oxidized low-density lipoprotein (OxLDL) and this may induce alveolar bone loss through toll-like receptor (TLR) 2 and 4. The purpose of this study was to investigate the effects of dyslipidemia on osteoclast differentiation associated with TLR2 and TLR4 in periodontal tissues using a rat dyslipidemia (apolipoprotein E deficient) model.
Methods: Levels of plasma OxLDL, and the cholesterol and phospholipid profiles in plasma lipoproteins were compared between apolipoprotein E-deficient rats (16-week-old males) and wild-type (control) rats. In the periodontal tissue, we evaluated the changes in TLR2, TLR4, receptor activator of nuclear factor kappa B ligand (RANKL) and tartrate resistant acid phosphatase (TRAP) expression.
Results: Apolipoprotein E-deficient rats showed higher plasma levels of OxLDL than control rats (p&lt;0.05), with higher plasma levels of total cholesterol (p&lt;0.05) and LDL-cholesterol (p&lt;0.05) and lower plasma levels of high-density lipoprotein cholesterol (p&lt;0.05). Their periodontal tissue also exhibited a higher ratio of RANKL-positive cells and a higher number of TRAP-positive osteoclasts than control rats (p&lt;0.05). Furthermore, periodontal gene expression of TLR2, TLR4 and RANKL was higher in apolipoprotein E-deficient rats than in control rats (p&lt;0.05).
Conclusion: These findings underscore the important role for TLR2 and TLR4 in mediating the osteoclast differentiation on alveolar bone response to dyslipidemia.

DOI： 10.1186/1476-511X-12-1

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Dental caries is correlated with knowledge of comprehensive food education in Japanese university students 国際誌

Daisuke Ekuni, Takaaki Tomofuji, Shinsuke Mizutani, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Azusa Kojima, Yoshiaki Iwasaki, Manabu Morita

ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION   22 ( 2 )   312 - 318   2013年

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記述言語：英語   出版者・発行元：H E C PRESS, HEALTHY EATING CLUB PTY LTD  

In Japan, the "Food Education Basic Law" (The Basic Law on Shokuiku, food education) was enacted in 2005. The comprehensive food education programs, namely Shokuiku, aim to improve dietary practices to reduce lifestyle-related diseases. Dental caries is one of the diseases associated with inappropriate dietary habits. Thus, food education may influence the prevalence of dental caries. However, there are no data regarding the association between public based-food education and dental caries. The aim of the present study was to investigate the relationship between knowledge about comprehensive food education (Shokuika) and dental caries experience in Japanese university students. A total of 2,184 students (1,240 men, 944 women), aged 18-20 years, were examined. They had attended the Shokuiku program while in junior/senior high school. The numbers of teeth present, and decayed, missing and filled teeth (DMFT) (dental caries experience) were recorded. Additional information was collected via a questionnaire regarding knowledge about food education, dietary habits and oral health behavior. Of the students, 315 men (20.7%) and 345 women (52.8%) reported that they know and can explain the meaning and content of the word "Shokuiku". After adjusting for potential confounding factors, subjects who did not have knowledge about Shokuiku had higher adjusted odds ratio (OR) for dental caries experience (DMFT &gt;0) than those who had (adjusted OR, 1.23; 95% CI, 1.02-1.48; p&lt;0.05). These observations revealed that having knowledge about comprehensive food education in university students correlates with low prevalence of dental caries.

DOI： 10.6133/apjcn.2013.22.2.13

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頭頸部がん患者における予後推定栄養指数と口腔内状態との関係

丸山貴之, 友藤孝明, 水谷慎介, 江國大輔, 森田 学

口腔衛生学会雑誌   63 ( 4 )   321 - 327   2013年

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記述言語：日本語   出版者・発行元：有限責任中間法人日本口腔衛生学会  

頭頸部がん治療の予後を知るうえで,小野寺の予後推定栄養指数(O-PNI)は重要な指標であるが,O-PNIと栄養摂取器官である口腔内の状態との関連について検討したものはない.本研究の目的は,頭頸部がん患者におけるO-PNIと口腔内状態との関連を調べることである.嚥下機能への影響を考慮するため,がんの発生部位を口腔内と口腔外に分け,それぞれのO-PNIと口腔内状態との関連について比較検討した.岡山大学病院にて頭頸部がんと診断を受けたがん治療開始前の150名を分析対象とした.がんの部位,進行度(ステージ),body mass index,飲酒経験,喫煙量,肝機能,腎機能,C反応性タンパク(CRP),口腔内指標を調べた.口腔内外それぞれのがんにおいて,O-PNIと各指標との重回帰分析を行い,O-PNIとの関連因子について検討した.重回帰分析の結果,口腔がん患者においてはO-PNIと年齢(p=0.017),平均クリニカルアタッチメントレベル(p=0.049),CRP(p<0.001)との間に関連を認めた.一方,口腔がん以外の頭頸部がん患者においてはO-PNIとCRP(p=0.005)との間に関連を認めたが,口腔内指標との関連は認められなかった.以上より,頭頸部がん患者におけるO-PNIを左右する因子として,口腔内のがん患者については歯周状態が関連しているのに対し,口腔外のがん患者については口腔内指標との関連はなかった.

DOI： 10.5834/jdh.63.4_321

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その他リンク： http://search.jamas.or.jp/link/ui/2014003705

Self-reported aural symptoms, headache and temporomandibular disorders in Japanese young adults

Rahena Akhter, Manabu Morita, Daisuke Ekuni, Nur Mohammad Monsur Hassan, Michiko Furuta, Reiko Yamanaka, Yoshizo Matsuka, David Wilson

BMC Musculoskeletal Disorders   14   2013年

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記述言語：英語  

Background: To investigate the associations of aural symptoms, headache and depression with the presence of temporomandibular disorder (TMD) symptoms in a young adult population in Japan. Methods. A personal interview survey was conducted on first-year university students (n = 1,930) regarding symptoms of TMD, aural problems, headache, shoulder pain and depression. Logistic regression was applied to assess the associations of these problems with the presence of TMD symptoms after controlling for age and gender. Results: Among the 1,930 students, 543 students exhibited TMD symptoms and were classified into 7 groups: clicking only (Group I, n = 319), pain in the TMJ only (Group II, n = 21), difficulty in mouth opening only (Group III, n = 18), clicking and pain (Group IV, n = 29), clicking and difficulty in mouth opening (Group V, n = 48), difficulty in mouth opening and pain (Group VI, n = 11), and combination of three symptoms (Group VII, n = 97). The control group (n = 1,387) were subjects without any TMD symptoms. After adjusting for age and gender, a strong association was observed between TMD symptoms (Group II and IV) and tinnitus (OR = 12.1 and 13.2, respectively). TMD symptoms (Group I, II and III) were also associated with vertigo and headache. Otalgia and depression were significantly associated with the presence of clicking only. Conclusions: TMD symptoms were significantly correlated to aural symptoms and headache. A functional evaluation of the stomatognathic system should be considered in subjects with unexplained aural symptoms and headache. © 2013 Akhter et al.
licensee BioMed Central Ltd.

DOI： 10.1186/1471-2474-14-58

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Hydrogen-rich water prevents lipid deposition in the descending aorta in a rat periodontitis model 国際誌

Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Shinsuke Mizutani, Azusa Kojima, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   57 ( 12 )   1615 - 1622   2012年12月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: Periodontitis has been causally linked to atherosclerosis, which is mediated by the oxidative stress. As hydrogen-rich water (HW) scavenges reactive oxygen species (ROS), we hypothesized that HW could prevent lipid deposition induced by periodontitis in the aorta. The aim of this study was to investigate the effects of HW on the initiation of atherosclerosis in a rat periodontitis model.
Design: Eighteen 8-wk-old male Wistar rats were divided into three groups of six rats; the periodontitis group, periodontitis + HW group and the no treatment (control) group. In the periodontitis and periodontitis + HW groups, periodontitis was induced using a ligature for 4 wk, while the periodontitis + HW group was given water containing 800-1000 mu g/L hydrogen during the 4-wk experimental period.
Results: In the periodontitis group, lipid deposition in the descending aorta was observed. The periodontitis group also showed significant higher serum levels for ROS and oxidised low-density lipoprotein-cholesterol (ox-LDL) (1.7 and 1.4 times, respectively), and higher aortic expression levels of nitrotyrosine and hexanoyl-lysine (HEL) (7.9 and 16.0 times, respectively), as compared to the control group (p &lt; 0.05). In the periodontitis + HW group, lipid deposition was lower. Lower serum levels of ROS and ox-LDL (0.46 and 0.82 times, respectively) and lower aortic levels of nitrotyrosine and HEL (0.27 and 0.19 times, respectively) were observed in the periodontitis + HW group than in the periodontitis group (p &lt; 0.05).
Conclusions: HW intake may prevent lipid deposition in the rat aorta induced by periodontitis by decreasing serum ox-LDL levels and aortic oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2012.04.013

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Gum chewing modulates heart rate variability under noise stress 国際誌

Daisuke Ekuni, Takaaki Tomofuji, Noriko Takeuchi, Manabu Morita

ACTA ODONTOLOGICA SCANDINAVICA   70 ( 6 )   491 - 496   2012年12月

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記述言語：英語   出版者・発行元：TAYLOR & FRANCIS LTD  

Objective. Gum chewing may relieve stress, although this hypothesis has not been proven. Heart-rate variability (HRV) is commonly used to measure stress levels. However, it is not known if gum chewing modulates HRV under acute stress. The aim of this study was to investigate the effects of gum chewing on HRV under acute stress. Materials and methods: A crossover study involving 47 non-smoking healthy subjects, aged 22-27 years, was carried out. The subjects received a stress procedure with gum chewing (GS group) and without gum chewing (S group). Additionally, the other 20 subjects were allocated to the gum chewing without stress group (G group). The GS and S groups were exposed to noise for 5 min (75 dBA) as stress. Before and after stress exposure/gum chewing, participants completed the state portion of the State-Trait Anxiety Inventory (STAI-s) and a single Stress Visual Analogue Scale (VAS) measurement. HRV measurement was performed before and during stress/gum chewing for 5 min. Results. After the stress procedure, VAS score significantly increased in the GS and S groups. During the stress procedure, the GS group showed a significantly lower level of high frequency (HF) and higher levels of low frequency (LF) and LF/HF than the S group. However, there were no significant differences in the scores of the state portion of the State-Trait Anxiety Inventory (STAI-s) and VAS between the two stress groups. Conclusions. These findings suggest that gum chewing modulates HRV, but may not relieve acute stress caused by noise.

DOI： 10.3109/00016357.2011.640275

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岡山大学新入生の自覚的口腔健康観に影響する因子の検討

児島 梓, 江國 大輔, 友藤 孝明, 東 哲司, 入江 浩一郎, 水谷 慎介, 森田 学

岡山歯学会雑誌   31 ( 2 )   97 - 97   2012年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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日本とカンボジアの若年者におけるソーシャル・キャピタルと口腔の健康の関係

古田 美智子, 野中 愛恵, 江國 大輔, 森田 学, 高尾 総司, 山下 喜久

日本公衆衛生学会総会抄録集   71回   583 - 583   2012年10月

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記述言語：日本語   出版者・発行元：日本公衆衛生学会  

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Relationship Between Serum Albumin Concentration and Periodontal Condition in Patients With Head and Neck Cancer 国際誌

Takayuki Maruyama, Reiko Yamanaka, Aya Yokoi, Daisuke Ekuni, Takaaki Tomofuji, Nobuyoshi Mizukawa, Tomoo Onoda, Motoharu Eguchi, Manabu Morita

JOURNAL OF PERIODONTOLOGY   83 ( 9 )   1110 - 1115   2012年9月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Serum albumin concentration is known to be an independent predictor of survival in head and neck cancer. The previous studies suggested relationships between serum albumin concentration and oral health status in populations without serious systemic disorders. However, these relationships remain unclear in patients with head and neck cancer. The purpose of this study is to investigate the relationship between serum albumin concentration and oral health status in patients with head and neck cancer.
Methods: Fifty individuals diagnosed with primary head and neck cancer, 25 individuals with normal serum albumin concentration (&gt;= 3.85 g/dL), and 25 age- and sex-matched individuals with lower serum albumin concentration (&lt;3.85 g/dL) were analyzed. General status, including cancer stage, body mass index, drinking and smoking habits, and biochemical serum markers, were evaluated. Oral health status, including periodontal condition and occlusion tooth pairs, were also evaluated.
Results: Mean clinical attachment level (CAL) and C-reactive protein in the lower serum albumin concentration group were greater than those in the normal serum albumin concentration group (P = 0.009 and P = 0.002, respectively). However, there were no significant differences in any other oral and/or serum parameters between the two serum albumin groups. A logistic regression model showed that mean CAL was significantly associated with high or low levels of serum albumin concentration (odds ratio = 9.752; 95% confidence interval = 1.702 to 55.861; P = 0.011).
Conclusions: This study suggests an association between periodontal disease and serum albumin concentration in patients with head and neck cancer. Longitudinal studies are necessary to examine the causal relationship between serum albumin concentration and periodontal condition. J Periodontol 2012;83:1110-1115.

DOI： 10.1902/jop.2011.110536

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Effects of self-efficacy on oral health behaviours and gingival health in university students aged 18- or 19-years-old 国際誌

Shinsuke Mizutani, Daisuke Ekuni, Michiko Furuta, Takaaki Tomofuji, Koichiro Irie, Tetsuji Azuma, Azusa Kojima, Jun Nagase, Yoshiaki Iwasaki, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY   39 ( 9 )   844 - 849   2012年9月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Aim Although self-efficacy is known to affect various health-related practises, few studies have clearly examined how self-efficacy correlates with oral health behaviors or the oral health condition. We examined the relationship between gingivitis, oral health behaviors and self-efficacy in university students. Material & Methods A total of 2,111 students (1,197 males, 914 females) aged 18 and 19 years were examined. The degree of gingivitis was expressed as the percentage of bleeding on probing (%BOP). Additional information was collected via a questionnaire regarding oral health behaviors (daily frequency of tooth-brushing, use of dental floss and regular check-up). Self-efficacy was assessed using the Self-Efficacy Scale for Self-care (SESS). Path analysis was used to test pathways from self-efficacy to oral health behaviors and %BOP. Results In the final structural model, self-efficacies were related to each other, and they affected oral health behaviors. Good oral health behaviors reduced dental plaque and calculus, and lower levels of dental plaque and calculus resulted in lower %BOP. Conclusion Higher self-efficacy correlated with better oral health behaviours and gingival health in university students. Improving self-efficacy may be beneficial for maintaining good gingival health in university students. To prevent gingivitis, the approach of enhancing self-efficacy in university students would be useful.

DOI： 10.1111/j.1600-051X.2012.01919.x

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Self-reports of eating quickly are related to a decreased number of chews until first swallow, total number of chews, and total duration of chewing in young people 国際誌

Daisuke Ekuni, Michiko Furuta, Noriko Takeuchi, Takaaki Tomofuji, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   57 ( 7 )   981 - 986   2012年7月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: The validity of a self-questionnaire about eating quickly remains unclear. If a significant relationship between subjective and objective methods to evaluate eating quickly can be confirmed, then the subjective method can be widely and reliably used in many fields. This study investigated relationships between subjective and objective methods to evaluate eating quickly and also numerically characterized the kinesis of eating quickly in young people.
Design: One hundred and thirteen students (44 males and 69 females; mean age 22.8 +/- 2.0 years) were selected. All subjects completed written questionnaires, and number of chews until first swallow, total duration of chewing, number of chews, chewing rate and bite size were measured using test products (a Japanese cracker and rice ball).
Results: Both male and female subjects who reported eating quickly showed a significantly lower number of chews until first swallow (Japanese cracker), a lower number of chews overall (rice ball), and a shorter total duration of chewing (rice ball) than other subjects. There was no difference in chewing rate between subjects who ate quickly or not.
Conclusions: These findings suggest that using test products, self-reports of eating quickly are related to a decreased number of chews until first swallow, total number of chews, and total duration of chewing, but not chewing rate, and that a self-reported questionnaire to evaluate eating rate is valid in young people. (C) 2012 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2012.02.001

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小学生における永久歯の萠出状況の報告

横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

岡山歯学会雑誌   31 ( 1 )   39 - 40   2012年6月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

岡山歯学会雑誌   31 ( 1 )   34 - 35   2012年6月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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ラット歯肉コラーゲンの加齢変化に対するトマリナの効果

友藤 孝明, 入江 浩一郎, 江國 大輔, 遠藤 康正, 粕山 健太, 東 哲司, 玉木 直文, 森田 学

日本抗加齢医学会総会プログラム・抄録集   12回   209 - 209   2012年6月

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記述言語：日本語   出版者・発行元：(一社)日本抗加齢医学会  

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Loss of claudin-1 in lipopolysaccharide-treated periodontal epithelium

T. Fujita, J. D. Firth, M. Kittaka, D. Ekuni, H. Kurihara, E. E. Putnins

JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   222 - 227   2012年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and Objective: The epithelial barrier is a critical component of innate immunity and provides protection against microbial invasion. Claudin-1, a tight junction protein, is known to contribute to the epithelial cell barrier. An experimentally induced rat periodontal disease model was used to study the effects of lipopolysaccharide (LPS) on the expression of tight junction-associated molecule genes in the junctional epithelium.
Material and Methods: LPS was applied for 8 wk in the gingival sulcus, and junctional epithelium was collected by laser-capture microdissection and subjected to microarray analysis.
Results: Microarray analysis identified that expression of the claudin-1 gene was decreased in the epithelium by chronic LPS challenge. Immunohistochemical analysis confirmed the expression of claudin-1 protein in junctional epithelium and that 8 wk of chronic LPS topical application significantly reduced claudin-1 expression. The effect of LPS on claudin-1 protein expression was validated using a porcine junctional epithelial cell culture Transwell model. The epithelial barrier, as measured using transmembrane resistance, was significantly reduced after 3 wk of LPS challenge and this was associated with a decreased level of expression of claudin-1 protein.
Conclusion: These results confirm that the initiation of experimental periodontal disease is associated with reduction in the expression of claudin-1 gene and protein. This decreased level of a critical tight junction protein may result in the disruption of barrier function and may play an important role in the initiation of periodontal disease.

DOI： 10.1111/j.1600-0765.2011.01424.x

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Effects of topical application of inorganic polyphosphate on tissue remodeling in rat inflamed gingiva

K. Kasuyama, T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, M. Morita

JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   159 - 164   2012年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and Objective: Inorganic polyphosphate [poly(P)] is a biopolymer found in almost all cells and tissues, and which promotes tissue remodeling. However, there is limited information on how poly(P) affects the connective tissue in inflamed gingiva. This study examined the effects of topical application of poly(P) on gingival connective tissue and its remodeling in a rat periodontitis model.
Material and Methods: Male Wistar rats (n = 36, 8 wk of age) were used in this 6-wk study. The rats were divided into six groups of six rats each. The control group received no treatment. In the other groups, periodontitis was ligatureinduced for 4 wk. After 4 wk, the rats with periodontitis were further divided into five groups, and were left untreated (periodontitis group) or subjected to topical application of oral rinses containing 0, 0.1, 1 or 5% poly(P) for 2 wk.
Results: The periodontitis and 0% poly(P) groups showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue than the control group (p &lt; 0.05). In contrast, groups treated with more than 1% poly(P) exhibited a lower density of polymorphonuclear leukocytes (p &lt; 0.05) and a higher density of collagen than the periodontitis and 0% poly(P) groups (p &lt; 0.05). A higher expression of fibroblast growth factor-2 was observed in the gingiva of rats treated with 1% poly(P) than in those treated with 0% poly(P) (p &lt; 0.05).
Conclusion: Topical application of poly(P) may induce connective tissue remodeling, contributing to improvement of inflamed gingiva in rats.

DOI： 10.1111/j.1600-0765.2011.01414.x

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Effects of topical application of inorganic polyphosphate on tissue remodeling in rat inflamed gingiva

K. Kasuyama, T. Tomofuji, D. Ekuni, T. Azuma, K. Irie, Y. Endo, M. Morita

JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   159 - 164   2012年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and Objective: Inorganic polyphosphate [poly(P)] is a biopolymer found in almost all cells and tissues, and which promotes tissue remodeling. However, there is limited information on how poly(P) affects the connective tissue in inflamed gingiva. This study examined the effects of topical application of poly(P) on gingival connective tissue and its remodeling in a rat periodontitis model.
Material and Methods: Male Wistar rats (n = 36, 8 wk of age) were used in this 6-wk study. The rats were divided into six groups of six rats each. The control group received no treatment. In the other groups, periodontitis was ligatureinduced for 4 wk. After 4 wk, the rats with periodontitis were further divided into five groups, and were left untreated (periodontitis group) or subjected to topical application of oral rinses containing 0, 0.1, 1 or 5% poly(P) for 2 wk.
Results: The periodontitis and 0% poly(P) groups showed a higher density of polymorphonuclear leukocytes and a lower density of collagen in gingival tissue than the control group (p &lt; 0.05). In contrast, groups treated with more than 1% poly(P) exhibited a lower density of polymorphonuclear leukocytes (p &lt; 0.05) and a higher density of collagen than the periodontitis and 0% poly(P) groups (p &lt; 0.05). A higher expression of fibroblast growth factor-2 was observed in the gingiva of rats treated with 1% poly(P) than in those treated with 0% poly(P) (p &lt; 0.05).
Conclusion: Topical application of poly(P) may induce connective tissue remodeling, contributing to improvement of inflamed gingiva in rats.

DOI： 10.1111/j.1600-0765.2011.01414.x

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Social capital and self-rated oral health among young people 国際誌

Michiko Furuta, Daisuke Ekuni, Soshi Takao, Etsuji Suzuki, Manabu Morita, Ichiro Kawachi

COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY   40 ( 2 )   97 - 104   2012年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Objectives: A few studies have revealed the impact of neighborhood social capital on oral health among young people. We sought to examine the associations of social capital in three settings (families, neighborhoods, and schools) with self-rated oral health among a sample of college students in Japan. Methods: Cross-sectional survey of 967 students in Okayama University, aged 18 and 19 years, was carried out. Logistic regression was used to examine the associations of poor self-rated oral health with perceptions of social capital, adjusting for self-perceived household income category and oral health behaviors. Results: The prevalence of subjects with poor self-rated oral health was 22%. Adjusted for gender, self-perceived household income category, dental fear, toothbrush frequency, and dental floss use, poor self-rated oral health was significantly associated with lower level of neighborhood trust [odds ratio (OR) 2.22; 95% confidence interval (CI): 1.40-3.54] and lower level of vertical trust in school (OR 1.71; 95% CI: 1.05-2.80). Low informal social control was unexpectedly associated with better oral health (OR 0.54; 95% CI: 0.34-0.85). Conclusion: The association of social capital with self-rated oral health is not uniform. Higher trust is associated with better oral health, whereas higher informal control in the community is associated with worse oral health.

DOI： 10.1111/j.1600-0528.2011.00642.x

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Social capital and self-rated oral health among young people 国際誌

Michiko Furuta, Daisuke Ekuni, Soshi Takao, Etsuji Suzuki, Manabu Morita, Ichiro Kawachi

COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY   40 ( 2 )   97 - 104   2012年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Objectives: A few studies have revealed the impact of neighborhood social capital on oral health among young people. We sought to examine the associations of social capital in three settings (families, neighborhoods, and schools) with self-rated oral health among a sample of college students in Japan. Methods: Cross-sectional survey of 967 students in Okayama University, aged 18 and 19 years, was carried out. Logistic regression was used to examine the associations of poor self-rated oral health with perceptions of social capital, adjusting for self-perceived household income category and oral health behaviors. Results: The prevalence of subjects with poor self-rated oral health was 22%. Adjusted for gender, self-perceived household income category, dental fear, toothbrush frequency, and dental floss use, poor self-rated oral health was significantly associated with lower level of neighborhood trust [odds ratio (OR) 2.22; 95% confidence interval (CI): 1.40-3.54] and lower level of vertical trust in school (OR 1.71; 95% CI: 1.05-2.80). Low informal social control was unexpectedly associated with better oral health (OR 0.54; 95% CI: 0.34-0.85). Conclusion: The association of social capital with self-rated oral health is not uniform. Higher trust is associated with better oral health, whereas higher informal control in the community is associated with worse oral health.

DOI： 10.1111/j.1600-0528.2011.00642.x

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Supplementation of broccoli or Bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet 国際誌

Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Tatsuo Yamamoto, Atsushi Ishikado, Takehiko Sato, Kayo Harada, Hirohisa Suido, Manabu Morita

NUTRITION RESEARCH   32 ( 4 )   301 - 307   2012年4月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

High-cholesterol diet enhances osteoclastic activity on alveolar bone by increasing serum lipid peroxidation. We hypothesized that supplementation with dietary antioxidants, such as found in broccoli and its fermented products, might suppress increases in serum lipid peroxidation, contributing to the inhibition of osteoclastic activity after high-cholesterol diet intake. The purpose of the present study was to investigate the effects of broccoli and fermented broccoli consumption on serum lipid peroxidation and osteoclast differentiation in alveolar bone of rats fed a high-cholesterol diet. In this 12-week study, rats were divided into 4 groups (n = 6/group): a control group (fed regular diet) and 3 experimental groups (fed a high-cholesterol [1% wt/wt] diet, or a high-cholesterol diet supplemented with either broccoli powder [5% wt/wt] or Bifidobacterium longum-fermented broccoli powder [5% wt/wt]). Serum hexanoyl-lysine (HEL) levels were measured as a parameter of lipid peroxidation. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in alveolar bone was enumerated to evaluate osteoclast differentiation. When compared with regular diet, the high-cholesterol diet increased serum HEL levels and resulted in a higher number of TRAP-positive osteoclasts at 12 weeks. The high-cholesterol diet supplemented with broccoli or B. longum-fermented broccoli showed lower levels of serum HEL and fewer TRAP-positive osteoclasts than the high-cholesterol diet at 12 weeks. In conclusion, consumption of broccoli, or its fermented product, inhibited the effects of a high-cholesterol diet on osteoclast differentiation in rat alveolar bone by suppressing serum lipid peroxidation. (C) 2012 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.nutres.2012.03.006

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Combined Effects of Hydrogen Sulfide and Lipopolysaccharide on Osteoclast Differentiation in Rats 国際誌

Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Ken Yaegaki, Manabu Morita

JOURNAL OF PERIODONTOLOGY   83 ( 4 )   522 - 527   2012年4月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll-like receptors (TLRs) 2 and 4, and hydrogen sulfide (H2S) induces osteoclast differentiation. If H2S activates TLRs, H2S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study is to examine the combined effects of sodium hydrogen sulfide (NaHS, an H2S donor drug) and LPS on osteoclast differentiation and TLR expression in rat periodontal tissue.
Methods: Twenty-eight male Wistar rats (8 weeks old) were divided into four groups (n = 7 per group): a control (no treatment) group and three experimental groups (NaHS group, LPS group, and a combination [NaHS + LPS] group). At 1 day after topical application of NaHS and/or Porphyromonas gingivalis LPS into the gingival sulcus of first molars, the number of tartrate-resistant acid phosphate (TRAP)-positive osteoclasts in the periodontal tissue was counted. Expression of TLR2 and TLR4 mRNAs and proteins in the gingival was also assessed.
Results: The number of TRAP-positive osteoclasts was significantly higher in the combination group than in any other group (P &lt; 0.01). The combination group had 11.0-fold higher TLR4 mRNA levels than the control group. TLR4 protein levels were also higher in the combination group than in the NaHS or LPS group. However, the TLR2 mRNA and protein levels were not significantly different in the combination group and the LPS group.
Conclusion: In rat periodontal tissue, NaHS and LPS had an additive effect on osteoclast differentiation through activation of the TLR4 pathway but not the TLR2 pathway. J Periodontol 2012;83:522-527.

DOI： 10.1902/jop.2011.110315

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Loss of claudin-1 in lipopolysaccharide-treated periodontal epithelium

T. Fujita, J. D. Firth, M. Kittaka, D. Ekuni, H. Kurihara, E. E. Putnins

JOURNAL OF PERIODONTAL RESEARCH   47 ( 2 )   222 - 227   2012年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and Objective: The epithelial barrier is a critical component of innate immunity and provides protection against microbial invasion. Claudin-1, a tight junction protein, is known to contribute to the epithelial cell barrier. An experimentally induced rat periodontal disease model was used to study the effects of lipopolysaccharide (LPS) on the expression of tight junction-associated molecule genes in the junctional epithelium.
Material and Methods: LPS was applied for 8 wk in the gingival sulcus, and junctional epithelium was collected by laser-capture microdissection and subjected to microarray analysis.
Results: Microarray analysis identified that expression of the claudin-1 gene was decreased in the epithelium by chronic LPS challenge. Immunohistochemical analysis confirmed the expression of claudin-1 protein in junctional epithelium and that 8 wk of chronic LPS topical application significantly reduced claudin-1 expression. The effect of LPS on claudin-1 protein expression was validated using a porcine junctional epithelial cell culture Transwell model. The epithelial barrier, as measured using transmembrane resistance, was significantly reduced after 3 wk of LPS challenge and this was associated with a decreased level of expression of claudin-1 protein.
Conclusion: These results confirm that the initiation of experimental periodontal disease is associated with reduction in the expression of claudin-1 gene and protein. This decreased level of a critical tight junction protein may result in the disruption of barrier function and may play an important role in the initiation of periodontal disease.

DOI： 10.1111/j.1600-0765.2011.01424.x

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コーヒー摂取と歯周病の関連

東 哲司, 友藤 孝明, 江國 大輔, 入江 浩一郎, 遠藤 康正, 粕山 健太, 森田 学

口腔衛生学会雑誌   62 ( 2 )   224 - 224   2012年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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咬合異常はラット海馬におけるβアミロイド量を増加させる

江國 大輔, 友藤 孝明, 入江 浩一郎, 東 哲司, 遠藤 康正, 粕山 健太, 森田 学

口腔衛生学会雑誌   62 ( 3 )   340 - 340   2012年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯周病メインテナンス期患者における歯周病の進行と全身の酸化ストレスとの関連

玉木 直文, 友藤 孝明, 江國 大輔, 森田 学

日本歯周病学会会誌   54 ( 春季特別 )   135 - 135   2012年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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歯肉コラーゲンの加齢変化に対するピクノジェノール配合歯磨剤の効果

粕山 健太, 友藤 孝明, 江國 大輔, 東 哲司, 入江 浩一郎, 遠藤 康正, 森田 学

日本歯周病学会会誌   54 ( 春季特別 )   132 - 132   2012年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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動物モデルを用いた基礎研究からヘルスプロモーションへ 歯周病の研究を行ってきた一人として

江國 大輔

口腔衛生学会雑誌   62 ( 2 )   144 - 145   2012年4月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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大学新入生の口腔健康状態の調査（性差および経年的変化の検討）．

児島 梓, 江國大輔, 古田美智子, 入江浩一郎, 東 哲司, 水谷慎介, 山中玲子, 友藤孝明, 森田 学, 山本龍生, 村上 隆, 山城 隆, 古賀 光, 岩崎良章, 清水幸登, 大西 勝, 小倉俊郎

CAMPUS HEALTH   49 ( 3 )   91 - 96   2012年

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記述言語：日本語   出版者・発行元：(公社)全国大学保健管理協会  

岡山大学の新入生を対象とした歯科健診の結果について、性差および経年的変化の検討を行った概要を報告する。2008年度から2011年度の新入生のうち18〜19歳の者を抽出し、総計5,097名(男性2,913名、女性2,184名)を対象に口腔健康状態を分析した。う蝕の保有者は全体の7.4%〜16.1%で、性差および経年的な傾向はなかった。一方、4mm以上の歯周ポケットを有する者の割合はCPIでは11.0%〜22.6%であり、経年的に軽症化にあった。男女比較では、プロービング時出血の割合、歯垢指数、歯石指数のすべてにおいて女性が有意に少なかった。さらに、智歯が1本も萠出していない者の割合は68.6%〜72.1%で、有意に女性が多かったが、年度間の差はなかった。不正咬合の割合は27.5%〜38.9%で経年的に減少し、歯科矯正治療経験者の割合は9.1%〜12.2%で経年的に増加していた。また、男女比較では両割合ともに女性が有意に多かった。以上より、大学新入生において、女性は男性に比べて歯周状態および口腔衛生状態が優れている一方で、不正咬合を有する者が多かった。また、経年的にはう蝕の保有者の割合には一定の傾向はなかったが、歯周状態と不正咬合は改善傾向にあった。今後も大学生の口腔の健康を保持・増進するために、歯科健診や保健指導を行う必要がある。(著者抄録)

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岡山大学新入生におけるう蝕経験の有無と食生活に対する自己効力感との関係．

水谷慎介, 江國大輔, 古田美智子, 友藤孝明, 入江浩一郎, 東 哲司, 児島 梓, 長瀬 純, 森田 学

口腔衛生学会雑誌   62 ( 4 )   403 - 409   2012年

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記述言語：日本語   出版者・発行元：有限責任中間法人日本口腔衛生学会  

これまでう蝕のリスク因子について多くの研究がなされてきたが,食生活に対する自己効力感が関係するかどうかは不明である.本研究の目的は,大学生新入生を対象に食生活に対する自己効力感とう蝕経験歯(DMF歯)の有無との関連を検討することである.対象者は2011年度岡山大学新入生のうち18,19歳の2,031名(男性1,159名,女性872名)である.アンケートにより,性別,食生活,フッ化物歯面塗布経験の有無,フッ化物配合歯磨剤の使用の有無および食生活に対する自己効力感に関する質問を行い,口腔内診査でDMF歯数を調べた.DMF歯の有無によって対象者を2群に分け,各項目について比較した.さらに,ロジスティック回帰分析を行った.ロジスティック回帰分析の結果.DMF歯の有無による2群比較において統計学的に有意であった変数は,性別(OR1.325,95%CI1.099-1.596,p=0.003),フッ化物歯面塗布経験の有無(OR0.765,95%CI0.634-0.924.p=0.005).食生活に対する自己効力感(OR0.813,95%CI0.672-0.982,p=0.032)および不規則な食事時間(OR1.250,95%CI1.005-1.554,p=0.045)の4項目であった.以上より,DMF歯の有無と食生活に対する自己効力感との間に有意な関連があった.

DOI： 10.5834/jdh.62.4_403

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早食いの是正に対するパンフレットと食事記録法の効果．

竹内倫子, 江國大輔, 友藤孝明, 森田 学

口腔衛生学会雑誌   62 ( 2 )   218 - 218   2012年

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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小学生における永久歯の萠出状況の報告

横井 彩, 山中 玲子, 江國 大輔, 丸山 貴之, 入江 浩一郎, 遠藤 康正, 水谷 慎介, 友藤 孝明, 森田 学

岡山歯学会雑誌   30 ( 2 )   87 - 87   2011年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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耳鼻咽喉科病棟看護師を対象に口腔ケアに関するアンケートの実施とその報告

志茂 加代子, 岡崎 惠子, 三浦 留美, 山中 玲子, 水川 展吉, 江国 大輔, 丸山 貴之, 横井 彩, 仲田 直樹, 武田 斉子, 喜田 沙音里, 森田 学

岡山歯学会雑誌   30 ( 2 )   82 - 82   2011年12月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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Relationships Between Eating Habits and Periodontal Condition in University Students 国際誌

Takaaki Tomofuji, Michiko Furuta, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yoshiaki Iwasaki, Manabu Morita

JOURNAL OF PERIODONTOLOGY   82 ( 12 )   1642 - 1649   2011年12月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Being overweight is a risk factor for peri odontitis. Unhealthy eating habits, which can induce overweight, may be involved in the development of periodontitis in young people. The present study aims to examine the relationships among overweight, eating habits, and the periodontal condition in Japanese university students.
Methods: We conducted a cross-sectional study of 801 university students (413 males and 388 females; age range: 18 to 25 years). Patients were classified as underweight (body mass index [BMI] &lt; 18.5 kg/m(2)), normal weight (BMI of 18.5 to 22.9 kg/m(2)), and overweight (BMI &gt;= 23 kg/m(2)). Students completed a questionnaire including items related to eating habits and underwent oral health examinations. Patients with a community periodontal index (CPI) of 0 to 2 were considered controls, and patients with a CPI &gt; 2 were considered to have periodontitis.
Results: The prevalence of underweight, normal weight, and overweight patients was 21%, 62%, and 17%, respectively. In overweight patients, the periodontitis risk was increased by the frequent consumption of fatty foods (adjusted odds ratio: 2.3; 95% confidence interval: 1.1 to 5.2; P &lt; 0.05) and reduced by the frequent consumption of vegetables (adjusted odds ratio: 0.2; 95% confidence interval: 0.1 to 0.7; P &lt; 0.01). In underweight and normal-weight groups, eating habits did not differ significantly according to the presence of periodontitis.
Conclusions: In overweight students, the frequent consumption of fatty foods and infrequent consumption of vegetables were associated with an increased risk of periodontitis. In underweight and normal-weight students, eating habits had little effect on the periodontal condition. J Periodontol 2071; 82:1642-1649.

DOI： 10.1902/jop.2011.110061

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Hydrogen-rich water attenuates experimental periodontitis in a rat model 国際誌

Kenta Kasuyama, Takaaki Tomofuji, Daisuke Ekuni, Naofumi Tamaki, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY   38 ( 12 )   1085 - 1090   2011年12月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Aim: Reactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen-rich water on a rat model of periodontitis.
Material & Methods: A ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen-rich water.
Results: The rats with periodontitis which were treated with pure water showed a time-dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis-induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen-rich water intake inhibited an increase in serum ROS level and lowered expression of 8-hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen-activated protein kinases, were less activated in periodontal lesions from hydrogen-rich water-treated rats as compared with pure water-treated rats.
Conclusion: Consuming hydrogen-rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

DOI： 10.1111/j.1600-051X.2011.01801.x

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Periodontal treatment decreases plasma oxidized LDL level and oxidative stress 国際誌

Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Manabu Morita

CLINICAL ORAL INVESTIGATIONS   15 ( 6 )   953 - 958   2011年12月

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記述言語：英語   出版者・発行元：SPRINGER HEIDELBERG  

Periodontitis induces excessive production of reactive oxygen species in periodontal lesions. This may impair circulating pro-oxidant/anti-oxidant balance and induce the oxidation of low-density lipoprotein (LDL) in blood. The purpose of this study was to monitor circulating oxidized LDL and oxidative stress in subjects with chronic periodontitis following non-surgical periodontal treatment. Plasma levels of oxidized LDL and oxidative stress in 22 otherwise healthy non-smokers with chronic periodontitis (mean age 44.0 years) were measured at baseline and at 1 and 2 months after non-surgical periodontal treatment. At baseline, chronic periodontitis patients had higher plasma levels of oxidized LDL and oxidative stress than healthy subjects (p &lt; 0.001). Periodontal treatment was associated with a significant reduction in plasma levels of oxidized LDL (oxLDL)(p &lt; 0.001) and oxidative stress (p &lt; 0.001). At 2 months after periodontal treatment, the degree of change in the oxLDL was positively correlated with that in the oxidative stress (r = 0.593, p = 0.004). These observations indicate that periodontitis patients showed higher levels of circulating oxLDL and oxidative stress than healthy subjects. In addition, improved oral hygiene and non-surgical periodontal treatment were effective in decreasing oxLDL, which was positively associated with a reduction in circulating oxidative stress.

DOI： 10.1007/s00784-010-0458-y

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Stage of hepatocellular carcinoma is associated with periodontitis 国際誌

Naofumi Tamaki, Akinobu Takaki, Takaaki Tomofuji, Yasumasa Endo, Kenta Kasuyama, Daisuke Ekuni, Tetsuya Yasunaka, Kazuhide Yamamoto, Manabu Morita

JOURNAL OF CLINICAL PERIODONTOLOGY   38 ( 11 )   1015 - 1020   2011年11月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Aim: Periodontitis induces overproduction of reactive oxygen species (ROS). This state increases circulating ROS levels and may affect hepatocellular carcinoma (HCC). The Japan Integrated Stage (JIS) score is a novel staging system for HCC. The objective of the present study was to compare JIS scores in HCC patients with and without periodontitis.
Material and Methods: We recruited 64 HCC patients comprising 31 chronic periodontitis subjects (HCC + P) and 33 periodontally healthy controls (HCC + H). Their JIS scores were recorded. Serum levels of reactive oxygen metabolites (ROM) from HCC + P, HCC + H and healthy age-and gender-matched subjects with healthy gingiva (control, n = 15) were also assessed for circulating ROS levels.
Results: The HCC + P and HCC + H groups had similar body mass index, habitual drinking and tobacco exposure data. The HCC + P group showed higher JIS scores than the HCC + H group (p = 0.027). Both the HCC + P and HCC + H groups had higher serum levels of ROM than controls (p &lt; 0.001), while serum levels of ROM in the HCC + P group were a further 25.8% higher than those in the HCC + H group (p &lt; 0.001).
Conclusion: HCC patients with periodontitis had higher JIS score and circulating ROS level than HCC patients without periodontitis.

DOI： 10.1111/j.1600-051X.2011.01777.x

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Relationships between periodontal inflammation, lipid peroxide and oxidative damage of multiple organs in rats

Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takayuki Maruyama, Tatsuo Yamamotto, Tatsuo Watanabe, Manabu Morita

BIOMEDICAL RESEARCH-TOKYO   32 ( 5 )   343 - 349   2011年10月

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記述言語：英語   出版者・発行元：BIOMEDICAL RESEARCH PRESS LTD  

Gingival response to periodontal inflammation generates excessive lipid peroxide and such a condition may augment systemic health through increased circulating lipid peroxide. The purpose of the present study was to investigate whether the generation of lipid peroxide in periodontal inflammation could induce tissue injury in the liver, heart, kidney and brain using a rat model. Twelve Wistar rats (8 week-old male) were divided into 2 groups: the periodontal inflammation group, receiving topical application of lipopolysaccharide and proteases to the gingival sulcus for 4 weeks, and the control group using instead pyrogen-free water. After blood samples were collected, specimens from the brain, heart, liver and kidney were resected to determine the concentration of 8-hydroxydeoxyguanosine (an indicator of oxidative DNA damage). Gingival and serum levels for hexanoyl-lysine were measured to evaluate lipid peroxide. Administration of lipopolysaccharide and proteases induced periodontal inflammation, with increasing gingival and serum levels of hexanoyl-lysine. The level of 8-hydroxydeoxyguanosine increased 2.27, 2.01, 1.49 and 1.40 times in mitochondrial DNA from the liver, heart, kidney and brain of rats with periodontal inflammation, respectively. The results reveal that excessive production of lipid peroxide following periodontal inflammation is involved in oxidative DNA damage of the brain, heart, liver and kidney.

DOI： 10.2220/biomedres.32.343

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Relationship between impacts attributed to malocclusion and psychological stress in young Japanese adults 国際誌

Daisuke Ekuni, Michiko Furuta, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Takashi Murakami, Takashi Yamashiro, Toshio Ogura, Manabu Morita

EUROPEAN JOURNAL OF ORTHODONTICS   33 ( 5 )   558 - 563   2011年10月

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記述言語：英語   出版者・発行元：OXFORD UNIV PRESS  

Identifying risk factors is important to prevent a wide range of health-damaging behaviours and to improve the quality of life of young people. The aim of this study was to investigate the relationship between impacts on daily performance attributed to malocclusion and psychological stress in healthy young Japanese adults.
Medical and oral health data were collected during a cross-sectional examination conducted by the Health Service Center of Okayama University. Systemically healthy non-smoking students aged 18 and 19 years (n = 641; 329 males and 312 females) were included. Malocclusion was defined using a modified version of the Index of Orthodontic Treatment Need (IOTN). The impacts on daily performance attributed to malocclusion and psychological stress were assessed using self-reported questionnaires, the condition-specific oral impacts on daily performances (CS-OIDP), and the Hopkins Symptoms Checklist. Mann-Whitney U- and chi-square tests and structural equation modelling (SEM) were used for statistical analysis.
Forty per cent of subjects had a malocclusion (n = 255). Subjects with impacts on daily performance had a significantly higher prevalence of malocclusion than those without impacts (P &lt; 0.001). SEM showed that psychological stress, especially interpersonal sensitivity and depression, was significantly correlated with CS-OIDP and malocclusion. Negative impacts on daily performance attributed to malocclusion may contribute to psychological stress in young Japanese adults.

DOI： 10.1093/ejo/cjq121

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Occlusal disharmony increases amyloid-β in the rat hippocampus.

Ekuni D, Tomofuji T, Irie K, Azuma T, Endo Y, Kasuyama K, Morita M

Neuromolecular Med   13 ( 3 )   197 - 203   2011年9月

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記述言語：英語  

DOI： 10.1007/s12017-011-8151-0

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硫化水素とLPSの混合塗布による破骨細胞分化への影響

入江 浩一郎, 江國 大輔, 友藤 孝明, 遠藤 康正, 粕山 健太, 八重垣 健, 森田 学

日本歯周病学会会誌   53 ( 秋季特別 )   112 - 112   2011年9月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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Effects of exercise training on gingival oxidative stress in obese rats 国際誌

Tetsuji Azuma, Takaaki Tomofuji, Yasumasa Endo, Naofumi Tamaki, Daisuke Ekuni, Koichiro Irie, Kenta Kasuyama, Tomo Kato, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   56 ( 8 )   768 - 774   2011年8月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet.
Design: Rats were divided into three groups (n = 14/group): one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill: 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidised-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress.
Results: The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean +/- SD; 413 +/- 64) than the control (333 +/- 12) at 4 weeks (p = 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 +/- 0.18) (p &lt; 0.05) and a lower GSH/GSSG ratio (17.0 +/- 3.1) (p &lt; 0.05) in gingival tissues, compared to the control (0.55 +/- 0.13 for 8-OHdG and 23.6 +/- 5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 +/- 103) (p&lt;0.001) and gingival 8-OHdG levels (0.69 +/- 0.17) (p = 0.012) than those without exercise training (1105 95 for ROM and 0.55 +/- 0.13 for 8-OHdG) at 8 weeks.
Conclusions: Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats. (C) 2011 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2011.01.008

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早食いの主観的評価と客観的評価の関連性

江國 大輔, 古田 美智子, 友藤 孝明, 森田 学

口腔衛生学会雑誌   61 ( 4 )   500 - 500   2011年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯周炎の状態と肝癌のステージとの関連についての研究

玉木 直文, 友藤 孝明, 遠藤 康正, 粕山 健太, 江國 大輔, 森田 学

口腔衛生学会雑誌   61 ( 4 )   453 - 453   2011年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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小学生における視力と口唇閉鎖力の関係(Relationship between Visual Acuity and Labial Closure Force in Japanese Elementary School Children)

山中 玲子, アクター・ラヘナ, 古田 美智子, 江國 大輔, 江崎 光恵, 山本 龍生, 岡崎 好秀, 丸山 貴之, 横井 彩, 森田 学

口腔衛生学会雑誌   61 ( 3 )   288 - 294   2011年7月

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記述言語：英語   出版者・発行元：(一社)日本口腔衛生学会  

口唇閉鎖力は、主に口輪筋の機能を反映している。口輪筋は発音や咀嚼などさまざまな口腔機能に関連している。口唇閉鎖力は咀嚼能力や不正咬合に関連がある。一方、裸眼視力1.0未満(&lt;20/20)の小学生は、日本においてこの20年間増え続けている。小学生の視力低下を予防することは、重要な課題である。視力は眼輪筋の強さに関連する可能性がある。眼輪筋は口輪筋とともに顔面筋の一つであり、両者ともに顔面神経の支配を受けており、頬筋などを介して機械的にもつながっており、協調して運動する。口輪筋の強さは、眼輪筋の強さを反映していると推測される。本研究では、口唇閉鎖力が視力に関連しているという仮説を立て、小学生の視力と口唇閉鎖力の関連性を横断的に検討することを目的とした。対象は岡山市内の7歳から12歳までの小学生396名(男子197名、女子199名)とした。口腔内状態と視力は、定期健康診断の結果を用いた。口唇閉鎖力は、LIP DE CUM/LDC-110(コスモ計器、東京)を用いて測定した。典型的な不正咬合を有する16名を除外し、高視力群(≧20/20)264名と低視力群(&lt;20/20)116名に分けた。統計分析には、Mann-Whitney U検定、χ2検定、ロジスティック回帰分析を用いた。高視力群では、口唇閉鎖力(p=0.024)と男子の割合(p=0.045)が有意に高く、年齢(p=0.001)とヘルマンの歯齢(p=0.002)が有意に低かった。性別、年齢、ヘルマンの歯齢で調整しても、低視力群では口唇閉鎖力が有意に低かった(OR 1.65、95% CI 1.04-2.64、p=0.004)。小学生において、視力と口唇閉鎖力には関連があることが示唆された。(著者抄録)

DOI： 10.5834/jdh.61.3_288

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Sex Differences in Gingivitis Relate to Interaction of Oral Health Behaviors in Young People 国際誌

Michiko Furuta, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Takaaki Tomofuji, Toshio Ogura, Manabu Morita

JOURNAL OF PERIODONTOLOGY   82 ( 4 )   558 - 565   2011年4月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Although many epidemiologic surveys have shown that gingivitis is more prevalent in males than in females, few studies have clearly explained what causes this difference. The objective of the present study is to explain the sex difference in gingivitis based on the interaction between oral health behaviors and related factors, such as knowledge, attitude, and lifestyle, in young people.
Methods: The study was comprised of 838 subjects (440 males and 398 females), aged 18 and 19 years. Gingivitis was assessed by the percentage of bleeding on probing (%BOP). Additional information was collected regarding oral hygiene status, oral health behaviors, and related factors. Structural equation modeling was used to test pathways from these factors to %BOP. Multiple-group modeling was also conducted to test for sex differences.
Results: Females had greater knowledge, a more positive attitude, a healthier lifestyle, and higher level of oral health behaviors than males. There were significant differences in the paths (i.e., from lifestyle, knowledge, and attitude to %BOP) through oral health behaviors and oral health status.
Conclusions: Sex-based differences in gingivitis in young people can be explained by oral health behaviors and hygiene status, which are influenced by lifestyle, knowledge, and attitude. To prevent gingivitis, different approaches to males and females may be useful. J Periodontol 2011;82:558-565.

DOI： 10.1902/jop.2010.100444

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Occlusal disharmony induces BDNF level in rat submandibular gland 国際誌

Koichiro Irie, Daisuke Ekuni, Takaaki Tomofuji, Tetsuji Azuma, Yasumasa Endo, Kenta Kasuyama, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   56 ( 1 )   35 - 40   2011年1月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objectives: Brain-derived neurotrophic factor (BDNF), which is produced in rat submandibular gland, is one of the most abundant neurotrophins in the central nervous system. It is generally accepted that occlusal disharmony causes stress. The purpose of the present study was to investigate whether occlusal disharmony-induced chronic stress affects BDNF levels and morphology in rat submandibular gland.
Design: Eight wks old male Wistar rats (n = 21) were randomly divided into three groups of 7 rats. In a control (C) group, the rats received no treatment for 8 wks. In a molar cusp-less (OD) group, maxillary molar cusps were cut off with a dental turbine at baseline and kept for 8 wks. In a molar cusp-less + recovered cusp (OR) group, maxillary molar cusps were cut off and then were recovered after 4 wks using resin material. After the experimental period, expression of BDNF mRNA and protein as well as histological findings were evaluated in the submandibular glands. The comparisons between the groups were made using the Mann-Whitney U test with Bonferroni correction.
Results: The OD group showed a significant increase in submandibular gland BDNF mRNA and protein expression after 8 wks, and plasma adrenocorticotropic hormone and corticosterone levels increased in a time-dependent manner. There were no significant differences in BDNF expression in the submandibular glands and in levels of plasma adrenocorticotropic hormone and corticosterone between the OR and C groups.
Conclusions: These results indicate that psychological stress induced by occlusal disharmony reversibly induces BDNF expression in the rat submandibular gland. (C) 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2010.09.001

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Relationship Between Decrease of Erythrocyte Count and Progression of Periodontal Disease in a Rural Japanese Population 国際誌

Tatsuo Yamamoto, Midori Tsuneishi, Michiko Furuta, Daisuke Ekuni, Manabu Morita, Yukio Hirata

JOURNAL OF PERIODONTOLOGY   82 ( 1 )   106 - 113   2011年1月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Limited research has been reported on the relationship between periodontal status and erythrocyte parameters. In the present study, longitudinal data from health checkups are used to clarify the relationship between periodontal disease progression and changes in parameters of erythrocytes.
Methods: In this prospective study, a total of 120 subjects (35 men and 85 women; age range: 30 to 63 years) participated in a comprehensive health screening and dental checkup in 2006 and 2007. Medical examinations, including anthropometric and manometric measurements, blood-chemistry tests, and oral examinations, were performed. Subjects with periodontal disease progression (i.e., the progression group) were defined based on the presence of &gt;= 1 tooth demonstrating a longitudinal loss of periodontal attachment &gt;= 3 mm or tooth loss during the study period. The Wilcoxon signed-rank, chi(2), Fisher exact, and Mann-Whitney U tests and stepwise logistic regression analysis were used for statistical analyses.
Results: The progression group comprised 30 subjects. A significant difference between the progression and non-progression groups was observed in changes of erythrocyte counts but not those of the body mass index, systolic and diastolic blood pressure, creatinine, fasting blood glucose, hemoglobin, hematocrit, triglycerides, high- and low-density lipoprotein cholesterol, total cholesterol, aspartate and alanine aminotransferase, and gamma-glutamyl transpeptidase. Stepwise logistic regression analysis showed that the progression of periodontal disease was associated with a change (year 2007 minus year 2006) of erythrocyte counts (adjusted odds ratio = 0.970; P = 0.009) after adjusting for age at baseline.
Conclusion: Within the limitations of this study, the progression of periodontal disease is associated with a decrease in erythrocyte counts in a rural Japanese population. J Periodontol 2011;82:106-113.

DOI： 10.1902/jop.2010.100211

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Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation

Takayuki Maruyama, Takaaki Tomofuji, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   56 ( 1 )   48 - 53   2011年1月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: This study examined the effects of a dentifrice containing green tea catechins on gingival oxidative stress and periodontal inflammation using a rat model.
Design: Twenty-four male Wister rats were randomly divided into four groups. The first group (Control group) received no treatment for 8 weeks. Periodontal inflammation was induced in the second group for 8 weeks. Periodontal inflammation was induced in the last two groups for 8 weeks and dentifrices with or without green tea catechins were topically applied to the gingival sulcus daily for 4 weeks prior to the end of the experimental period. Results: Rats that had experimental periodontal inflammation showed apical migration of the junctional epithelium, alveolar bone loss and inflammatory cell infiltration in the connective tissue subjacent to the junctional epithelium at 8 weeks, whilst the control group showed no pathologic changes. Topical application of a green tea catechin-containing dentifrice reduced inflammatory cell infiltration in the periodontal lesions to a greater degree than the control dentifrice at 8 weeks. The gingiva in which green tea catechin-containing dentifrice was applied also showed a lower level of expression of hexanoyl-lysine (a marker of lipid peroxidation), nitrotyrosine (a marker of oxidative protein damage), and tumour necrosis factor-a (an indicator of pro-inflammatory cytokines) at 8 weeks compared to gingiva in which the control dentifrice was applied.
Conclusions: Adding green tea catechins to a dentifrice may contribute to prevention of periodontal inflammation by decreasing gingival oxidative stress and expression of pro-inflammatory cytokines. (C) 2010 Elsevier Ltd. All rights reserved.

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新入生歯科健診からみえてきたこと

江國大輔, 古田美智子, 入江浩一郎, 東 哲司, 児島 梓, 水谷慎介, 山中玲子, 友藤孝明, 森田 学, 山本龍生, 古賀 光, 岩崎良章, 清水幸登, 大西 勝, 小倉俊郎

第41回中国・四国大学保健管理研究集会報告書   2011年

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Relationship between Malocclusion and Heart Rate Variability Indices in Young Adults A Pilot Study

D. Ekuni, N. Takeuchi, M. Furuta, T. Tomofuji, M. Morita

METHODS OF INFORMATION IN MEDICINE   50 ( 4 )   358 - 363   2011年

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記述言語：英語   出版者・発行元：SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN  

Objectives: Heart rate variability (HRV) has been used to assess sympathetic and parasympathetic modulation of heart rate. Chronic stress relates to reduced HRV. Malocclusion has effects on quality of life, which can lead to chronic stress. Therefore, we hypothesized that malocclusion, as chronic stress, may contribute to reduced HRV. The aim of this study was to investigate the relationship between malocclusion and HRV indices in healthy young adults.
Methods: Thirty-seven non-smoking healthy subjects, aged 22 to 25 years, were examined. Malocclusion was defined by Angle classification. HRV indices included root mean square of successive differences, low frequency (LF), high frequency (HF) and ratio of LF to HE The effects of malocclusion on quality of life and mental health were assessed using self-reported questionnaires, the condition-specific Oral Impacts on Daily Performances index (CS-OIDP) and the Hopkins Symptoms Checklist (HSCL), respectively.
Results: Significantly lower score of HF and higher heart rate (HR) level and CS-OIDR score were observed in subjects with malocclusion (n = 17) compared to those in the control subjects (n = 20) (P&lt;0.05). There was a positive correlation between HR and score of "anxiety" in HSCL (P &lt; 0.05).
Conclusions: The data showed an association between malocclusion and lower HRV. Based on our results, orthodontic treatment might contribute not only to improvement of oral esthetic and functional problems but also to improvement of stress and HRV indices.

DOI： 10.3414/ME10-01-0045

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Relationship between Malocclusion and Heart Rate Variability Indices in Young Adults A Pilot Study

D. Ekuni, N. Takeuchi, M. Furuta, T. Tomofuji, M. Morita

METHODS OF INFORMATION IN MEDICINE   50 ( 4 )   358 - 363   2011年

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記述言語：英語   出版者・発行元：SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN  

Objectives: Heart rate variability (HRV) has been used to assess sympathetic and parasympathetic modulation of heart rate. Chronic stress relates to reduced HRV. Malocclusion has effects on quality of life, which can lead to chronic stress. Therefore, we hypothesized that malocclusion, as chronic stress, may contribute to reduced HRV. The aim of this study was to investigate the relationship between malocclusion and HRV indices in healthy young adults.
Methods: Thirty-seven non-smoking healthy subjects, aged 22 to 25 years, were examined. Malocclusion was defined by Angle classification. HRV indices included root mean square of successive differences, low frequency (LF), high frequency (HF) and ratio of LF to HE The effects of malocclusion on quality of life and mental health were assessed using self-reported questionnaires, the condition-specific Oral Impacts on Daily Performances index (CS-OIDP) and the Hopkins Symptoms Checklist (HSCL), respectively.
Results: Significantly lower score of HF and higher heart rate (HR) level and CS-OIDR score were observed in subjects with malocclusion (n = 17) compared to those in the control subjects (n = 20) (P&lt;0.05). There was a positive correlation between HR and score of "anxiety" in HSCL (P &lt; 0.05).
Conclusions: The data showed an association between malocclusion and lower HRV. Based on our results, orthodontic treatment might contribute not only to improvement of oral esthetic and functional problems but also to improvement of stress and HRV indices.

DOI： 10.3414/ME10-01-0045

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A new portable monitor for measuring odorous compounds in oral, exhaled and nasal air 国際誌

Naofumi Tamaki, Kenta Kasuyama, Mitsue Esaki, Takara Toshikawa, Shun-Ichi Honda, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

BMC ORAL HEALTH   11 ( 1 )   15 - 15   2011年

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記述言語：英語   出版者・発行元：BIOMED CENTRAL LTD  

Background: The B/B Checker(R), a new portable device for detecting odorous compounds in oral, exhaled, and nasal air, is now available. As a single unit, this device is capable of detecting several kinds of gases mixed with volatile sulfur compounds (VSC) in addition to other odorous gasses. The purpose of the present study was to evaluate the effectiveness of the B/B Checker(R) for detecting the malodor level of oral, exhaled, and nasal air.
Methods: A total of 30 healthy, non-smoking volunteers (16 males and 14 females) participated in this study. The malodor levels in oral, exhaled, and nasal air were measured using the B/B Checker(R) and by organoleptic test (OT) scores. The VSCs in each air were also measured by gas chromatography (GC). Associations among B/B Checker(R) measurements, OT scores and VSC levels were analyzed using Spearman correlation coefficients. In order to determine the appropriate B/B Checker(R) level for screening subjects with malodor, sensitivity and specificity were calculated using OT scores as an identifier for diagnosing oral malodor.
Results: In oral and nasal air, the total VSC levels measured by GC significantly correlated to that measured by the B/B Checker(R). Significant correlation was observed between the results of OT scores and the B/B Checker(R) measurements in oral (r = 0.892, p &lt; 0.001), exhaled (r = 0.748, p &lt; 0.001) and nasal air (r = 0.534, p &lt; 0.001). The correlation between the OT scores and VSC levels was significant only for oral air (r = 0.790, p &lt; 0.001) and nasal air (r = 0.431, p = 0.002); not for exhaled air (r = 0.310, p = 0.096). When the screening level of the B/B Checker(R) was set to 50.0 for oral air, the sensitivity and specificity were 1.00 and 0.90, respectively. On the other hand, the screening level of the B/B Checker(R) was set to 60.0 for exhaled air, the sensitivity and specificity were 0.82 and 1.00, respectively.
Conclusion: The B/B Checker(R) is useful for objective evaluation of malodor in oral, exhaled and nasal air and for screening subjects with halitosis.

DOI： 10.1186/1472-6831-11-15

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Antioxidants and prevention of periodontal disease.

Global Research Network   2011年

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Oral malodorous compound induces osteoclast differentiation without receptor activator of nuclear factor κB ligand. 国際誌

Ii H, Imai T, Yaegaki K, Irie K, Ekuni D, Morita M

J Periodontol.   81 ( 11 )   1691 - 1697   2010年11月

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記述言語：英語  

DOI： 10.1902/jop.2010.100116

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Relationship between periodontal condition and arterial properties in an adult population in Japan

M. Furuta, T. Tomofuji, D. Ekuni, N. Tamaki, T. Yamamoto, T. Azuma, K. Irie, Y. Endo, S. Yamada, M. Morita

ORAL DISEASES   16 ( 8 )   781 - 787   2010年11月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Objective:
This study addressed the relationship between periodontal condition and second derivative of the finger photoplethysmogram (SDPTG) in Japanese adults.
Subject and methods:
The Community Periodontal Index (CPI) and SDPTG were recorded in 415 subjects (mean age: 44.0 years). For assessing SDPTG, we mainly focused on the ratio of the absolute value of the height of the early negative &apos;b&apos; wave and ratio of the late re-decreasing &apos;d&apos; wave to the height of the initial positive &apos;a&apos; wave, namely the b/a and d/a ratios.
Results:
The CPI score was positively correlated with the b/a ratio (P &lt; 0.001), and negatively correlated with the d/a ratio (P &lt; 0.001). Logistic regression analysis showed that subjects with CPI scores &gt;= 3 were more likely to have a higher level (male &gt;-0.69, female &gt;-0.64) of b/a ratio (Odds ratio = 1.7, P = 0.026) and lower level (male &lt;-0.29, female &lt;-0.32) of d/a ratio (Odds ratio = 2.2, P = 0.001) than those with CPI scores 0-2, after adjusting for age, gender, smoking status, pulse rate and presence of hypertension.
Conclusion:
There was a statistical association between the CPI scores and SDPTG indices in Japanese adults.

DOI： 10.1111/j.1601-0825.2010.01688.x

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小学生における視力と口唇閉鎖力の関係

山中 玲子, 古田 美智子, 江崎 光恵, 江國 大輔, 丸山 貴之, 岡崎 好秀, 森田 学

口腔衛生学会雑誌   60 ( 5 )   606 - 606   2010年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Relationship between the prognosis of periodontitis and occlusal force during the maintenance phase - a cohort study

N. Takeuchi, D. Ekuni, T. Yamamoto, M. Morita

JOURNAL OF PERIODONTAL RESEARCH   45 ( 5 )   612 - 617   2010年10月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background and Objective:
Few studies have longitudinally investigated the relationship between periodontal disease progression and occlusal factors in individual subjects during the maintenance phase of periodontal therapy. The aim of this cohort study was to investigate the relationship between biting ability and the progression of periodontal disease in the maintenance phase.
Material and Methods:
A total of 194 patients were monitored for 3 years during the maintenance phase of periodontal therapy. The subjects with disease progression (Progress group) were defined based on the presence of &gt;= 2 teeth demonstrating a longitudinal loss of proximal attachment of &gt;= 3 mm or tooth-loss experience as a result of periodontal disease during the study period. The subjects with high occlusal force were diagnosed as men who showed an occlusal force of more than 500 N and women who showed an occlusal force of more than 370 N. The association between biting ability and the progression of periodontitis was investigated using logistic regression analysis.
Results:
There were 83 subjects in the Progress group and 111 subjects in the Non-progress group. A backward, stepwise logistic regression model showed that the progression of periodontal disease was significantly associated with the presence of one or more teeth with a high clinical attachment level (CAL) of &gt;= 7 mm (odds ratio: 2.397; 95% confidence interval: 1.306-4.399) ( p = 0.005) and low occlusal force (odds ratio: 2.352; 95% confidence interval: 1.273-4.346) ( p = 0.006).
Conclusion:
The presence of one or more teeth with a high CAL of &gt;= 7 mm and low occlusal force might be possible risk factors for periodontal progression in the maintenance phase of periodontal therapy.

DOI： 10.1111/j.1600-0765.2010.01273.x

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歯周状態と加速度脈波との関連性についての検討

遠藤 康正, 友藤 孝明, 江國 大輔, 玉木 直文, 山本 龍生, 東 哲司, 入江 浩一郎, 森田 学

日本歯周病学会会誌   52 ( 秋季特別 )   97 - 97   2010年9月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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頭頸部がん患者における口腔内の状態と血清アルブミン値の関係

丸山 貴之, 山中 玲子, 横井 彩, 古田 美智子, 江國 大輔, 森田 学

口腔衛生学会雑誌   60 ( 4 )   461 - 461   2010年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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大学新入生における歯肉炎の性差についての検討

古田 美智子, 江國 大輔, 入江 浩一郎, 東 哲司, 加藤 朋, 山田 小百合, 友藤 孝明, 森田 学

口腔衛生学会雑誌   60 ( 4 )   364 - 364   2010年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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運動による肥満予防がラット歯周組織に及ぼす影響

東 哲司, 入江 浩一郎, 遠藤 康正, 粕山 健太, 加藤 朋, 江國 大輔, 玉木 直文, 友藤 孝明, 森田 学

口腔衛生学会雑誌   60 ( 4 )   414 - 414   2010年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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咬合異常は顎下腺BDNFに影響を及ぼす

入江 浩一郎, 江國 大輔, 友藤 孝明, 遠藤 康正, 東 哲司, 粕山 健太, 森田 学

日本抗加齢医学会総会プログラム・抄録集   10回   236 - 236   2010年5月

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記述言語：日本語   出版者・発行元：(一社)日本抗加齢医学会  

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Experimental Periodontitis Induces Gene Expression of Proinflammatory Cytokines in Liver and White Adipose Tissues in Obesity

Yasumasa Endo, Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

JOURNAL OF PERIODONTOLOGY   81 ( 4 )   520 - 526   2010年4月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Recent studies indicated that periodontitis induces systemic low-grade inflammation. The increase in systemic low-grade inflammation induced by periodontitis may alter the effects of obesity on the production of inflammatory molecules, including C-reactive protein (CRP), interleukin (IL)-6, and tumor necrosis factor-alpha (TNF-alpha), in the liver and white adipose tissue (WAT). The purpose of the present study is to investigate the effects of periodontitis on the expression of proinflammatory cytokines in the liver and WAT in obese Zucker rats.
Methods: Obese Zucker rats and their lean litter mates were divided into four groups of six rats each: lean Zucker rats without periodontitis (control group), lean Zucker rats with periodontitis (periodontitis group), obese Zucker rats without periodontitis (obesity group), and obese Zucker rats with periodontitis (combination group). Periodontitis was ligature induced for 4 weeks in the periodontitis and combination groups, whereas the other groups were left unligated.
Results: At 4 weeks, the gene expression for CRP, IL-6, and TNF-alpha in the liver and CRP and IL-6 in the WAT of combination groups was significantly higher than in each of the three groups. Serum TNF-alpha in the periodontitis and obesity groups was significantly higher than in the control group. Serum CRP and TNF-alpha in the combination group was significantly higher than in each of the three groups.
Conclusion: Systemic low-grade inflammation after experimental periodontitis was associated with increased gene expression for hepatic levels of TNF-alpha and CRP and adipose tissue levels of IL-6 and CRP in the obese-rat model. J Periodontol 2010;81:520-526.

DOI： 10.1902/jop.2009.090574

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Effects of periodontitis on aortic insulin resistance in an obese rat model

Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Tetsuji Azuma, Naofumi Tamaki, Toshihiro Sanbe, Yasumasa Endo, Tatsuo Yamamoto, Takashi Nishida, Manabu Morita

LABORATORY INVESTIGATION   90 ( 3 )   348 - 359   2010年3月

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記述言語：英語   出版者・発行元：NATURE PUBLISHING GROUP  

The combination of obesity and its associated risk factors, such as insulin resistance and inflammation, results in the development of atherosclerosis. However, the effects of periodontitis on atherosclerosis in an obese body remain unclear. The aim of the study was to investigate the effects of ligature-induced periodontitis in Zucker fatty rats on initiation of atherosclerosis by evaluating aortic insulin resistance. Zucker fatty rats (n = 24) were divided into two groups. In the periodontitis group, periodontitis was ligature-induced for 4 weeks, whereas the control group was left unligated. After the 4-week experimental period, descending aorta was used for measuring the levels of lipid deposits, immunohistochemical analysis, and evaluation of gene expression. Levels of serum C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), and insulin were also measured. Rats in the periodontitis group had significantly enhanced lipid deposits in the aorta, but not in the control group. Expression of suppressor of cytokine signaling 3, vascular cell adhesion molecule 1, reactive oxygen species, nitrotyrosine, and endothelin-1 in the periodontitis group was more intense than that in the control group. Significantly decreased levels of phosphatidylinositol 3-kinase (Pi3k) catalytic beta-polypeptide (Pi3kcb), Pi3kp85, and insulin receptor substrate 1 and 2 were observed in the periodontitis group. Levels of serum CRP and TNF-alpha were significantly increased in the periodontitis group. Under insulin-stimulated conditions, aorta in the periodontitis group altered the Akt phosphorylation. Periodontitis in obesity induced the initial stage of atherosclerosis and disturbed aortic insulin signaling.

DOI： 10.1038/labinvest.2009.141

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Effects of topical application of lipopolysaccharide and proteases on hepatic injury induced by high-cholesterol diet in rats

T. Yamamoto, T. Tomofuji, N. Tamaki, D. Ekuni, T. Azuma, T. Sanbe

JOURNAL OF PERIODONTAL RESEARCH   45 ( 1 )   129 - 135   2010年2月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

Background and Objective: Topical application of lipopolysaccharide and proteases to the gingival sulcus induced not only periodontal inflammation but also detectable liver changes in rats fed a normal diet. However, these changes in the liver were not sufficient to induce pathological consequences. The purpose of the present study was to investigate whether gingival inflammation-induced liver change would have more dramatic pathological consequences in rats fed a high-cholesterol diet compared with the effect of the high-cholesterol diet alone.
Material and Methods: Twenty-four male Wistar rats were divided into four groups. During an 8 week experimental period, two groups were fed a normal diet and the other two were fed a high-cholesterol diet containing 1% cholesterol (w/w) and 0.5% cholic acid (w/w). Four weeks prior to the end of the experimental period, one of each of the dietary groups received daily topical application of lipopolysaccharide and proteases to the gingival sulcus, while the other was treated with pyrogen-free water.
Results: In the rats without application of lipopolysaccharide and proteases, the serum level of hexanoyl-lysine, scores of steatosis and inflammation, and concentration of 8-hydroxydeoxyguanosine in liver of rats fed a high-cholesterol diet were higher than in those fed a normal diet. In rats fed a high-cholesterol diet, the scores of steatosis and inflammation and the concentration of 8-hydroxydeoxyguanosine in the liver of rats with application of lipopolysaccharide and proteases were higher than in those without.
Conclusion: In a rat model, application of lipopolysaccharide and proteases to the gingival sulcus augmented the effect of a high-cholesterol diet on steatosis, inflammation and oxidative damage in the liver.

DOI： 10.1111/j.1600-0765.2009.01212.x

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Effects of mechanical stimulation by a powered toothbrush on the healing of periodontal tissue in a rat model of periodontal disease

D. Ekuni, R. Yamanaka, T. Yamamoto, M. Miyauchi, T. Takata, T. Watanabe

JOURNAL OF PERIODONTAL RESEARCH   45 ( 1 )   45 - 51   2010年2月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

Background and Objective: Elimination of pathogens is the main aim of periodontal treatment; however, modulation of the host immune response should also be considered. This study aimed to evaluate the effects of mechanical stimulation on periodontal healing in rats.
Material and Methods: Before starting the experiment, lipopolysaccharide and proteases were applied once a day, for 4 wk, to both maxillary first molars of 30 rats to induce periodontal disease, and the application was stopped at the end of the 4-wk period. The experiment started immediately following this pretreatment. In the experiment, the left palatal gingiva was stimulated once daily using a powered toothbrush and the right gingiva served as a control (no mechanical stimulation). Pathological changes, and proliferation and cell death in periodontal tissues, were evaluated histometrically and immunohistochemically at baseline (0 wk), and at 1 and 3 wk of stimulation.
Results: The control showed a reduction of polymorphonuclear leukocyte infiltration in connective tissue and an increase in the numbers of gingival and periodontal ligament fibroblasts. Mechanical stimulation reduced polymorphonuclear leukocyte infiltration and the area of destroyed collagen in connective tissue, and increased the number of gingival fibroblasts; however, it had no effect on alveolar bone and root resorption or on the number of periodontal ligament fibroblasts.
Conclusion: Mechanical stimulation accelerated the healing of gingival inflammation by reducing the infiltration of polymorphonuclear leukocytes and enhancing fibroblast proliferation and collagen synthesis.

DOI： 10.1111/j.1600-0765.2009.01195.x

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Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands 国際誌

Daisuke Ekuni, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takaaki Tomofuji, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   55 ( 2 )   170 - 176   2010年2月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: Epidemiologic studies suggest a relationship between periodontitis and salivary gland dysfunction. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and pathological changes of submandibular glands.
Design: Fourteen male Wistar rats (8 weeks old) were divided into two groups (n = 7/group): a control group and periodontitis group. Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites, antioxidant and tumour necrosis factor (TNF)-alpha were measured at baseline, 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine were determined to evaluate oxidative damage of submandibular glands. Expression of TNF-alpha mRNA and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) as well as histological findings were also evaluated in the submandibular glands.
Results: The rats with experimental periodontitis showed increase in the levels of serum reactive oxygen metabolites and TNF-alpha, and a decrease of anti-oxidant power in a time-dependent manner. At 4 weeks, these rats also had significantly increased levels of 8-hydroxydeoxyguanosine and TNF-alpha, and increased number of TUNEL-positive cells and vacuolisation in the submandibular glands compared to the control rats.
Conclusions: Imbalance of circulating oxidative/anti-oxidative status may be involved in vacuolisation and apoptosis of submandibular glands in the rat periodontitis model. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2009.11.013

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Relationship between periodontal status and components of metabolic syndrome in a rural Japanese population.

Yamamoto T, Tsuneishi M, Furuta M, Koyama R, Ekuni D, Morita M, Hirata Y

口腔衛生学会雑誌   60   96 - 103   2010年

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ワークショップ「頭頸部がんチーム医療における口腔ケアの促進」と連携強化．

山中玲子, 丸山貴之, 横井 彩, 山田小百合, 玉木直文, 江國大輔, 友藤孝明, 森田 学

岡山歯学会雑誌   61   2010年

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メインテナンス期の歯周病患者における咬合力と歯周病進行との関係について

竹内倫子, 江國大輔, 山本龍生, 森田 学

岡山歯学会雑誌   29 ( 1 )   67 - 67   2010年

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記述言語：日本語   出版者・発行元：岡山歯学会  

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健康診査データにおけるメタボリックシンドロームと歯周炎の関係

山本 龍生, 恒石 美登里, 古田 美智子, 小山 玲子, 江國 大輔, 森田 学, 平田 幸夫

神奈川歯学   44 ( 抄録集 )   25 - 25   2009年12月

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記述言語：日本語   出版者・発行元：神奈川歯科大学学会  

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Preventive Effects of a Cocoa-Enriched Diet on Gingival Oxidative Stress in Experimental Periodontitis

Takaaki Tomofuji, Daisuke Ekuni, Koichiro Irie, Tetsuji Azuma, Yasumasa Endo, Naofumi Tamaki, Toshihiro Sanbe, Jun Murakami, Tatsuo Yamamoto, Manabu Morita

JOURNAL OF PERIODONTOLOGY   80 ( 11 )   1799 - 1808   2009年11月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Oxidative stress affects the progression of periodontitis. Cocoa is a rich source of flavonoids with antioxidant properties, which could suppress gingival oxidative stress in periodontal lesions. The purpose of the present study was to investigate the effects of a cocoa-enriched diet on gingival oxidative stress in a rat-periodontitis model.
Methods: In this 4-week study, rats were divided into three groups (n = 8/group): a control group (fed a regular diet) and two periodontitis groups (fed a regular diet or cocoa-enriched diet [10% of food intake]). Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites were measured at baseline and 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio were determined to evaluate gingival oxidative damage and antioxidant status, respectively.
Results: Rats with experimental periodontitis that were fed a regular diet showed an increase in the level of serum reactive oxygen metabolites in a time-dependent manner. These rats also had an increased 8-hydroxydeoxyguanosine level and decreased reduced/oxidized glutathione ratio in the gingival tissue, inducing alveolar bone loss and polymorphonuclear leukocyte infiltration. Although experimental periodontitis was induced in the rats fed a cocoa-enriched diet, they did not show impairments in serum reactive oxygen metabolite level and gingival levels for 8-hydroxydeoxyguanosine and reduced/oxidized glutathione ratio. Alveolar bone loss and polymorphonuclear leukocyte infiltration after ligature placement were also inhibited by cocoa intake.
Conclusion: Consuming a cocoa-enriched diet could diminish periodontitis-induced oxidative stress, which, in turn, might suppress the progression of periodontitis. J Periodontol 2009;80:1799-1808.

DOI： 10.1902/jop.2009.090270

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急性期病院における頭頸部癌患者への口腔ケア

山中 玲子, 小山 玲子, 江國 大輔, 古田 美智子, 丸山 貴之, 山本 龍生, 森田 学

口腔衛生学会雑誌   59 ( 5 )   609 - 610   2009年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Lipopolysaccharide-Induced Epithelial Monoamine Oxidase Mediates Alveolar Bone Loss in a Rat Chronic Wound Model 国際誌

Daisuke Ekuni, James D. Firth, Tarun Nayer, Takaaki Tomofuji, Toshihiro Sanbe, Koichiro Irie, Tatsuo Yamamoto, Takashi Oka, Zhenzi Liu, Juergen Vielkind, Edward E. Putnins

AMERICAN JOURNAL OF PATHOLOGY   175 ( 4 )   1398 - 1409   2009年10月

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記述言語：英語   出版者・発行元：AMER SOC INVESTIGATIVE PATHOLOGY, INC  

Reactive oxygen species (ROS) production is an antimicrobial response to pathogenic challenge that may, in the case of persistent infection, have deleterious effects on the tissue of origin. A rat periodontal disease model was used to study ROS-induced chronic epithelial inflammation and bone loss. Lipopolysaccharide (LPS) was applied for 8 weeks into the gingival sulcus, and histological analysis confirmed the onset of chronic disease. junctional epithelium was collected from healthy and diseased animals using laser-capture microdissection, and expression microarray analysis was performed. Of 19,730 genes changed in disease, 42 were up-regulated &gt;= 4-fold. Three of the top 10 LPS-induced genes, monoamine oxidase B (MAO/B) and flavin-containing monooxygenase 1 and 2, are implicated in ROS signaling. LPS-associated induction of the ROS mediator H(2)O(2) as well as MAO/B and tumor necrosis factor (TNF)-alpha levels were validated in the rat histological sections and a porcine junctional epithelial cell culture model. Topical MAO inhibitors significantly counteracted LPS-associated elevation of H(2)O(2) production and TNF-alpha expression in vivo and in vitro, inhibited disease-associated apical migration and proliferation of junctional epithelium and inhibited induced systemic H(2)O(2) levels and alveolar bone loss in vivo. These results suggest that LPS induces chronic wounds via elevated MAO/B-mediated increases in H(2)O(2) and TNF-alpha activity by epithelial cells and is further associated with more distant effects on systemic oxidative stress and alveolar bone loss. (Am J Pathol 2009,175:1398-1409; DOI: 10.2353/ajpath.2009.090108)

DOI： 10.2353/ajpath.2009.090108

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A single application of hydrogen sulphide induces a transient osteoclast differentiation with RANKL expression in the rat model 国際誌

Koichiro Irie, Daisuke Ekuni, Tatsuo Yamamoto, Manabu Morita, Ken Yaegaki, Hisataka Ii, Toshio Imai

ARCHIVES OF ORAL BIOLOGY   54 ( 8 )   723 - 729   2009年8月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: Oral malodor is mainly attributed to volatile sulphur compounds (VSCs) such as hydrogen sulphide (H2S), methyl mercaptan and dimethyl sulphide. VSC accelerate periodontal soft tissue destruction. However, there is little information about the potential role of H2S in alveolar bone loss. The purpose of this animal study was to examine the effects of sodium hydrogen sulphide (NaHS), H2S donor drug, on osteoclast differentiation in rat periodontal tissue.
Design: Twenty-four male Wistar rats (8 weeks old) were divided into four groups: a control group and three experimental groups, which were examined at 3 h, 1 day, and 3 days after topical application of 3 mu l NaHS (l M in physiological saline) into the gingival sulcus of rat first molar. Expression of tumour necrosis factor (TNF)-alpha, RANKL, NF-kappa B and tartrate-resistant acid phosphatase (TRAP) was evaluated in the periodontal tissue.
Results: Three hours after NaHS application, TNF-alpha expression increased in the periodontal ligament. The numbers of RANKL-positive osteoblasts and TRAP-positive osteoclasts significantly increased progressively with time and reached a maximum level after 1 day. Significant up-regulation of RANKL and NF-kappa B mRNA was observed at 3 h after NaHS application.
Conclusions: H2S application caused a transient increase of osteoclast differentiation with up-regulation of RANKL expression in osteoblasts. H2S, which is primarily responsible for halitosis, may also contribute to alveolar bone resorption through RANKL expression. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2009.05.006

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Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis

D. Ekuni, T. Tomofuji, T. Sanbe, K. Irie, T. Azuma, T. Maruyama, N. Tamaki, J. Murakami, S. Kokeguchi, T. Yamamoto

JOURNAL OF PERIODONTAL RESEARCH   44 ( 4 )   434 - 442   2009年8月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

Background and Objective:
Periodontitis is a risk factor for the development of atherosclerosis. Recent studies indicate that oxidative mechanisms, including lipid peroxidation, are involved not only in periodontitis but also in atherosclerosis. Lipid peroxidation may play an important role in the pathogenesis of atherosclerosis, particularly during its earliest stages. The purpose of this study was to investigate the relationship between lipid peroxidation induced by periodontitis and the initiation of atherosclerosis.
Material and Methods:
Sixteen rats were randomly divided into two groups of eight rats each. Periodontitis was ligature-induced for 4 wk in the experimental group, whereas the control group was left untreated. After the experimental period, the mandibular first molar regions were resected and then subjected to histological analysis and measurement of hexanoyl-lysine expression as an indicator of lipid peroxidation. Descending aorta was used for measuring the levels of hexanoyl-lysine, reactive oxygen species and lipid deposits, and for real-time polymerase chain reaction microarray analysis. The level of hexanoyl-lysine was also measured in serum.
Results:
In the experimental group, the levels of hexanoyl-lysine in periodontal tissue and serum increased. Only aorta samples in the experimental group showed lipid accumulation, with increased expression of hexanoyl-lysine, reactive oxygen species and oxidative stress-related genes (including nitric oxide synthases 2 and 3), whereas the superoxide dismutase 1 gene level was down-regulated.
Conclusion:
In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.

DOI： 10.1111/j.1600-0765.2008.01122.x

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Relation of dietary preference to bite force and occlusal contact area in Japanese children

R. Yamanaka, R. Akther, M. Furuta, R. Koyama, T. Tomofuji, D. Ekuni, N. Tamaki, T. Azuma, T. Yamamoto, E. Kishimoto

JOURNAL OF ORAL REHABILITATION   36 ( 8 )   584 - 591   2009年8月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

P&gt;The purpose of this study was to examine the relationship of dietary preference to bite force and occlusal contact area in Japanese elementary school children. A total of 348 children, aged 7-12 years, from two public elementary schools located in Okayama Prefecture, Japan, participated in the study. Clinical examination included decayed, missing and filled teeth (dmft and DMFT), and total numbers of deciduous and permanent teeth. Bite force and occlusal contact area were measured using a pressure-detecting sheet. Dietary preference was assessed using a questionnaire in which the answers were given in like/dislike form. Mann-Whitney U-test and multiple logistic regression analysis were applied to analyse the data. In multiple logistic regression analysis after adjustment for age, gender and total number of teeth present, children who liked cabbage and celery showed significantly higher bite force (P = 0 center dot 05 and P &lt; 0 center dot 01, respectively) than those who disliked these. Children who liked cabbage and celery also showed higher occlusal contact area (P &lt; 0 center dot 05 and P &lt; 0 center dot 01, respectively) than those who disliked these. The Japanese elementary school children who liked hard foods such as cabbage and celery showed higher bite force and higher occlusal contact area than those who disliked these foods. A positive attitude towards harder food items might contribute to healthy development of the masticatory apparatus.

DOI： 10.1111/j.1365-2842.2009.01971.x

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Effects of Obesity on Gingival Oxidative Stress in a Rat Model 国際誌

Takaaki Tomofuji, Tatsuo Yamamoto, Naofumi Tamaki, Daisuke Ekuni, Tetsuji Azuma, Toshihiro Sanbe, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Jun Murakami, Susumu Kokeguchi, Manabu Morita

JOURNAL OF PERIODONTOLOGY   80 ( 8 )   1324 - 1329   2009年8月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Studies indicate a correlation between obesity and periodontitis. Oxidative stress is involved in the progression of periodontitis. The purpose of this study was to investigate the effects of obesity on gingival oxidative stress in a rat periodontitis model.
Methods: The obese Zucker rats (n = 14) and their lean littermates (n = 14) were each divided into two groups of seven rats. In one of each group, periodontitis was induced by ligature for 4 weeks, whereas the other group was left unligated. The level of 8-hydroxydeoxyguanosine and the ratio of reduced/oxidized glutathione were determined to examine gingival oxidative stress. The serum level of reactive oxygen metabolites and the gingival gene-expression pattern related to oxidative/metabolic stress, inflammation, and cell behavior were also evaluated.
Results: The obese rats weighed more than the lean rats at 4 weeks. Compared to lean rats, obese rats had enhanced gingival 8-hydroxydeoxyguanosine levels and a decreased ratio of reduced/oxidized glutathione in the gingival tissue, with increasing serum reactive oxygen metabolites. However, there were no significant differences in the degree of alveolar bone loss between lean and obese rats, except for teeth with and without ligatures in both rats. In addition, the periodontal lesion in obese rats showed higher 8-hydroxydeoxyguanosine levels and polymorphonuclear leukocyte infiltration than the inflamed ones in lean rats, with downregulation of multiple cytochrome P450 gene expression.
Conclusions: Obesity induced gingival oxidative stress with increasing serum reactive oxygen metabolites in rats. In the periodontal lesion, gene expressions related to a capacity for xenobiotic detoxification were downregulated in the obese model. J Periodontol 2009;80:1324-1329.

DOI： 10.1902/jop.2009.080621

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Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis

D. Ekuni, T. Tomofuji, T. Sanbe, K. Irie, T. Azuma, T. Maruyama, N. Tamaki, J. Murakami, S. Kokeguchi, T. Yamamoto

JOURNAL OF PERIODONTAL RESEARCH   44 ( 4 )   434 - 442   2009年8月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

Background and Objective:
Periodontitis is a risk factor for the development of atherosclerosis. Recent studies indicate that oxidative mechanisms, including lipid peroxidation, are involved not only in periodontitis but also in atherosclerosis. Lipid peroxidation may play an important role in the pathogenesis of atherosclerosis, particularly during its earliest stages. The purpose of this study was to investigate the relationship between lipid peroxidation induced by periodontitis and the initiation of atherosclerosis.
Material and Methods:
Sixteen rats were randomly divided into two groups of eight rats each. Periodontitis was ligature-induced for 4 wk in the experimental group, whereas the control group was left untreated. After the experimental period, the mandibular first molar regions were resected and then subjected to histological analysis and measurement of hexanoyl-lysine expression as an indicator of lipid peroxidation. Descending aorta was used for measuring the levels of hexanoyl-lysine, reactive oxygen species and lipid deposits, and for real-time polymerase chain reaction microarray analysis. The level of hexanoyl-lysine was also measured in serum.
Results:
In the experimental group, the levels of hexanoyl-lysine in periodontal tissue and serum increased. Only aorta samples in the experimental group showed lipid accumulation, with increased expression of hexanoyl-lysine, reactive oxygen species and oxidative stress-related genes (including nitric oxide synthases 2 and 3), whereas the superoxide dismutase 1 gene level was down-regulated.
Conclusion:
In a ligature-induced periodontitis rat model, increased lipid peroxidation was found in serum and aorta as well as in periodontal tissue. Atherosclerosis-related gene expression and histological changes were also stimulated. Periodontitis-induced lipid peroxidation in the aorta may be involved in the early stage of atherosclerosis.

DOI： 10.1111/j.1600-0765.2008.01122.x

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肥満モデルラットにおける動脈のインスリン抵抗性に対する歯周炎の影響

江國 大輔, 友藤 孝明, 入江 浩一郎, 粕山 健太, 馬越 通弘, 東 哲司, 玉木 直文, 山本 龍生, 森田 学

口腔衛生学会雑誌   59 ( 4 )   383 - 383   2009年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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大学新入生における歯周疾患と口腔保健行動との関係および口腔保健行動の性差

古田 美智子, 江國 大輔, 入江 浩一郎, 東 哲司, 加藤 朋, 山田 小百合, 友藤 孝明, 森田 学

口腔衛生学会雑誌   59 ( 4 )   477 - 477   2009年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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口臭原因物質の破骨細胞RAW264の分化におよぼす影響

伊井 久貴, 今井 敏夫, 八重垣 健, 入江 浩一郎, 江國 大輔, 森田 学

口腔衛生学会雑誌   59 ( 4 )   393 - 393   2009年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Effects of improvement in periodontal inflammation by toothbrushing on serum lipopolysaccharide concentration and liver injury in rats

Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Tetsuji Azuma, Naofumi Tamaki, Koichiro Irie, Takayuki Maruyama, Tatsuo Yamamoto, Tatsuo Watanabe, Mutsumi Miyauchi, Takashi Takata

ACTA ODONTOLOGICA SCANDINAVICA   67 ( 4 )   200 - 205   2009年7月

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記述言語：英語   出版者・発行元：TAYLOR & FRANCIS LTD  

Objective. Periodontitis increases the serum lipopolysaccharide level, contributing to liver injury. Toothbrushing improves periodontitis and may also affect serum lipopolysaccharide concentration and periodontitis-induced liver injury. The purpose of the present study was to examine whether the improvement in periodontal inflammation by toothbrushing clinically affects the serum lipopolysaccharide level and hepatic pathological changes in rat periodontitis. Material and methods. Thirty male Wistar rats were divided into 5 groups, 2 groups receiving topical application of pyrogen-free water to the gingival sulcus for 4 or 8 weeks. The next 2 groups received topical application of lipopolysaccharide and proteases for 4 or 8 weeks. The last group received topical application of lipopolysaccharide and proteases for 8 weeks, and the palatal gingiva was brushed with a powered toothbrush once a day for 4 weeks prior to the end of the experimental period. Results. Topical application of lipopolysaccharide and proteases induced not only periodontal inflammation but also an elevation in the serum lipopolysaccharide concentration, with increasing hepatic inflammation, steatosis and 8-hydroxydeoxyguanosine levels in a time-dependent mariner. The rats that received gingival stimulation showed decreased polymorphonuclear leukocyte infiltration and collagen loss levels in the periodontal lesions. Furthermore, this group also showed a decrease in serum lipopolysaccharide concentration and hepatic inflammation, steatosis and 8-hydroxydeoxyguanosine levels, compared with the group receiving no treatment. Conclusions. Toothbrushing promoted healing of periodontal lesions, decreased serum lipopolysaccharide concentration and suppressed liver injury in a rat periodontitis model.

DOI： 10.1080/00016350902794818

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アンケートによる歯周疾患スクリーニングの有効性

小山 玲子, 玉木 直文, 丸山 貴之, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 山本 龍生, 森田 学

岡山歯学会雑誌   28 ( 1 )   88 - 88   2009年6月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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Short-Term Effects of Non-Surgical Periodontal Treatment on Plasma Level of Reactive Oxygen Metabolites in Patients With Chronic Periodontitis 国際誌

Naofumi Tamaki, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Tatsuo Yamamoto, Manabu Morita

JOURNAL OF PERIODONTOLOGY   80 ( 6 )   901 - 906   2009年6月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Elevated levels of blood reactive oxygen species (ROS) are associated with the severity of periodontitis. Therefore, improvement of periodontitis may result in a decrease in blood ROS. However, it is unclear how periodontal treatment affects blood ROS. Recently, reactive oxygen metabolites (ROMs) were recognized as a useful measure of blood ROS. The aim of this longitudinal study was to investigate the effect of non-surgical periodontal treatment on plasma ROMs in patients with chronic periodontitis.
Methods: Nineteen subjects with chronic periodontitis (mean age: 46.8 years) were monitored at baseline (prior to scaling and root planing) and I and 2 months after therapy. Dental health parameters were evaluated, and plasma was obtained at these time points from patients and controls (19 subjects without periodontitis; mean age: 45.3 years), The plasma ROM level was determined using a spectrophotometric technique.
Results: At baseline, patients with chronic periodontitis had higher plasma ROM level (441.8 +/- 71.1 Carratelli units) than the control subjects (324.4 +/- 34.0 Carratelli units; P &lt; 0.01). Probing depth, clinical attachment level, and bleeding on probing in patients with chronic periodontitis showed a significant improvement 2 months after non-surgical periodontal treatment, and this was accompanied by a significant reduction in plasma ROM level (P &lt; 0.01).
Conclusions: In patients with chronic periodontitis, nonsurgical periodontal treatment was effective at improving clinical parameters and reducing plasma ROMs. The improvement in chronic periodontitis by non-surgical periodontal treatment might offer clinical benefits by decreasing blood ROS. J Periodontol 2009;80:907-906.

DOI： 10.1902/jop.2009.080640

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高コレステロール食によるラットの血清過酸化脂質の増加と歯槽骨における破骨細胞の分化 ビタミンC摂取の影響

三部 俊博, 友藤 孝明, 江國 大輔, 東 哲司, 入江 浩一郎, 玉木 直文, 山本 龍生, 森田 学

岡山歯学会雑誌   28 ( 1 )   94 - 94   2009年6月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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模擬患者参加型の医療面接を組み合わせたテュートリアル教育の試み

吉田 登志子, 玉村 亮, 曽我 賢彦, 江國 大輔, 黒坂 寛, 大前 正範, 長谷川 浩一, 松村 誠士, 北山 滋雄

岡山歯学会雑誌   28 ( 1 )   83 - 84   2009年6月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress

Daisuke Ekuni, Takaaki Tomofuji, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

ARCHIVES OF ORAL BIOLOGY   54 ( 5 )   495 - 502   2009年5月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: Periodontitis has been causally linked to cardiovascular disease, which is mediated through the oxidative stress induced by periodontitis. Since vitamin C has been suggested to limit oxidative damage, we hypothesized that vitamin C intake may reduce endothelial oxidative stress induced by periodontitis in the aorta. The aim of this study was to investigate the effects of vitamin C intake on the initiation of atherosclerosis in a ligature-induced rat periodontitis model.
Design: Eighteen 8-week-old-male Wistar rats were divided into three groups of six rats and all rats received daily fresh water and powdered food through out the 6-week study. In the vitamin C and periodontitis groups, periodontitis was ligature-induced for the first 4 weeks. In the vitamin C group, rats were given distilled water containing 1 g/L vitamin C for the 2 weeks after removing the ligature.
Results: In the periodontitis group, there was lipid deposition in the descending aorta and significant increases of serum level of hexanoyl-lysine (HEL), and aortic levels of nitrotyrosine expression, HEL expression and 8-hydroxydeoxyguanosine (8-OHdG) compared to the control group. Vitamin C intake significantly increased plasma vitamin C level and GSH:GSSG ratio (178% and 123%, respectively), and decreased level of serum HEL and aortic levels of nitrotyrosine, HEL and 8-OHdG (23%, 87%, 84%, and 38%, respectively).
Conclusions: These results suggest that vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. (C) 2009 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2009.02.006

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Periodontitis and increase in circulating oxidative stress

Takaaki Tomofuji, Koichiro Irie, Toshihiro Sanbe, Tetsuji Azuma, Daisuke Ekuni, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

Japanese Dental Science Review   45 ( 1 )   46 - 51   2009年5月

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記述言語：英語   掲載種別：書評論文，書評，文献紹介等  

Reactive oxygen species (ROS) are products of normal cellular metabolism. However, excessive production of ROS oxidizes DNA, lipids and proteins, inducing tissue damage. Studies have shown that periodontitis induces excessive ROS production in periodontal tissue. When periodontitis develops, ROS produced in the periodontal lesion diffuse into the blood stream, resulting in the oxidation of blood molecules (circulating oxidative stress). Such oxidation may be detrimental to systemic health. For instance, previous animal studies suggested that experimental periodontitis induces oxidative damage of the liver and descending aorta by increasing circulating oxidative stress. In addition, it has been revealed that clinical parameters in chronic periodontitis patients showed a significant improvement 2 months after periodontal treatment, which was accompanied by a significant reduction of reactive oxygen metabolites in plasma. Improvement of periodontitis by periodontal treatment could reduce the occurrence of circulating oxidative stress. Furthermore, recent studies indicate that the increase in circulating oxidative stress following diabetes mellitus and inappropriate nutrition damages periodontal tissues. In such cases, therapeutic approaches to systemic oxidative stress might be necessary to improve periodontal health. © 2009 Japanese Association for Dental Science.

DOI： 10.1016/j.jdsr.2008.12.002

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Retrospective study of teeth with a poor prognosis following non-surgical periodontal treatment 国際誌

Daisuke Ekuni, Tatsuo Yamamoto, Noriko Takeuchi

JOURNAL OF CLINICAL PERIODONTOLOGY   36 ( 4 )   343 - 348   2009年4月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

Ekuni D, Yamamoto T, Takeuchi N. Retrospective study of teeth with a poor prognosis following non-surgical periodontal treatment. J Clin Periodontol 2009; 36: 343-348. doi: 10.1111/j.1600-051X.2009.01373.x.
The aim of this retrospective study was to assess teeth with a poor prognosis and the proximal periodontium of adjacent teeth, and to identify the risk factors associated with the loss of teeth with a poor prognosis following non-surgical periodontal treatment.
Teeth with a poor prognosis (n=113), teeth adjacent to those of poor prognosis (n=105) and non-adjacent teeth (n=51) were evaluated in 25 non-smoking patients who had received supportive periodontal treatment for 5-16 years following non-surgical periodontal treatment at a university hospital.
Probing pocket depth (PPD), percentage of alveolar bone loss, presence of tooth mobility and bleeding on probing in all teeth improved significantly after treatment. Logistic regression analysis showed that loss of teeth with a poor prognosis depended on the initial deepest PPD, tooth mobility and multi-rooted tooth.
Teeth with a poor prognosis did not affect the proximal periodontium of the adjacent teeth, and progression of periodontal disease in these teeth and adjacent teeth can be prevented by non-surgical periodontal treatment in non-smokers. The risk factors for loss of teeth with a poor prognosis were the initial deepest PPD, tooth mobility and multi-rooted tooth.

DOI： 10.1111/j.1600-051X.2009.01373.x

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ラット歯肉の酸化ストレスに対する肥満の影響

入江 浩一郎, 友藤 孝明, 三部 俊博, 玉木 直文, 江國 大輔, 馬越 道弘, 粕山 健太, 東 哲司, 村上 純, 苔口 進, 山本 龍生, 森田 学

日本抗加齢医学会総会プログラム・抄録集   9回   222 - 222   2009年4月

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記述言語：日本語   出版者・発行元：(一社)日本抗加齢医学会  

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ラット歯周炎における歯肉の酸化ストレスに対する高ココア食摂取の影響

友藤 孝明, 三部 俊博, 入江 浩一郎, 江國 大輔, 東 哲司, 遠藤 康正, 玉木 直文, 山本 龍生, 森田 学

日本抗加齢医学会総会プログラム・抄録集   9回   220 - 220   2009年4月

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記述言語：日本語   出版者・発行元：(一社)日本抗加齢医学会  

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液体歯磨剤「クリーンデンタル薬用リンス」の抗炎症・抗菌成分のラット歯周炎モデルへの効果

山本 龍生, 友藤 孝明, 江國 大輔, 東 哲司, 三部 俊博, 入江 浩一郎, 粕山 健太, 馬越 道弘, 森田 学

日本歯周病学会会誌   51 ( 春季特別 )   135 - 135   2009年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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Vitamin C intake inhibits serum lipid peroxidation and osteoclast differentiation on alveolar bone in rats fed on a high-cholesterol diet 国際誌

Toshihiro Sanbe, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Naofumi Tamaki, Tatsuo Yamamoto, Manabu Morita

ARCHIVES OF ORAL BIOLOGY   54 ( 3 )   235 - 240   2009年3月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: A high-cholesterol diet stimulates osteoclast differentiation, which may be induced by increased serum lipid peroxidation. The inhibition of serum lipid peroxidation by vitamin C may offer beneficial effects on osteoclast differentiation including increased expression of receptor activator of nuclear factor (NF)-kappa B ligand (RANKL) and NF-kappa B. This study investigated the effects of vitamin C intake on RANKL and NF-kappa B expression in periodontal tissue of rats fed a high-cholesterol diet.
Design: Twenty-four rats (8 weeks old) were divided into four groups: a control group (fed a regular diet) and three experimental groups (fed a high-cholesterol diet supplemented with 0,1 and 2 g/I vitamin C/day) in this 12-week study. Vitamin C was provided by its addition to drinking water. As an index of serum lipid peroxidation, hexanoyl-lysine (HEL) level was determined by a competitive enzyme-linked immunosorbent assay method. Immunohistological analysis was performed to evaluate RANKL and NF-kappa B expression on the alveolar bone surface. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts was also counted.
Results: Feeding a high-cholesterol diet increased not only the serum HEL level but also the number of TRAP-positive osteoclasts on the alveolar bone surface, with an increase in RANKL and NF-kappa B expression on alveolar bone surface. intake of vitamin C reduced the serum HEL level and osteoclast differentiation, with decreasing RANKL and NF-kappa B expression.
Conclusions: Vitamin C intake could suppress osteoclast differentiation, including RANKL and NF-kappa B expression on the alveolar bone surface, by decreasing serum lipid peroxidation in rats fed a high-cholesterol diet. (c) 2008 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2008.11.001

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Validity of a Questionnaire for Periodontitis Screening of Japanese Employees

Tatsuo Yamamoto, Reiko Koyama, Naofumi Tamaki, Takayuki Maruyama, Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Tetsuji Azuma, Manabu Morita

JOURNAL OF OCCUPATIONAL HEALTH   51 ( 2 )   137 - 143   2009年3月

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記述言語：英語   出版者・発行元：JAPAN SOC OCCUPATIONAL HEALTH  

Validity of a Questionnaire for Periodontitis Screening of Japanese Employees: Tatsuo YAMAMOTO, et al. Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences-Objectives: Workplaces are suitable for screening for periodontal disease effectively. However, the majority of workplaces do not have a screening program. One possible reason may be that pocket probing, which is commonly used for the screening, needs qualified examiners and is time-consuming. In this study, the validity of a self-reported questionnaire was assessed for screening of periodontitis in 50- to 59-yr-old male employees in Japan. Methods: A total of 250 people were asked to complete a self-administered questionnaire regarding signs and symptoms of periodontal disease, and experience of periodontal treatment, followed by a clinical examination by a dentist. Subjects with at least one tooth having a clinical attachment loss of 7 mm or more were defined as periodontitis patients. Results: Thirteen percent of the subjects were diagnosed as having periodontitis. Logistic regression selected 4 questions, "Are you a current or past smoker?", "Have your gums bled recently?", "Do you think that you can see more roots of teeth than in the past?", and "Have you ever been told that you need periodontal or gum treatment?" as potential predictors of periodontitis. When subjects with at least 3 "yes" responses to the 4 questions were separated from the others, the subjects with periodontitis were separated most effectively (showing the highest sensitivity + specificity: 1.524) from those without. The area under the receiver operating characteristic curve was 0.81. Conclusions: These results suggest that the self-reported questions are useful for screening of periodontitis in 50- to 59-yr-old Japanese workers.

DOI： 10.1539/joh.L8108

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Effects of vitamin C intake on gingival oxidative stress in rat periodontitis

Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

FREE RADICAL BIOLOGY AND MEDICINE   46 ( 2 )   163 - 168   2009年1月

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記述言語：英語   出版者・発行元：ELSEVIER SCIENCE INC  

Increased levels of oxidative stress due to excessive production of reactive oxygen species are involved in the pathogenesis of periodontitis. Studies Suggest a negative association between plasma vitamin C level and the severity of periodontitis. We hypothesized that increases in plasma vitamin C levels after vitamin C intake might clinically reduce gingival oxidative stress in a rat periodontitis model. A ligature was placed around rat mandibular molars for 4 weeks to induce periodontitis, and the rats were then given drinking water with or without 1 g/L vitamin C for 2 weeks after the ligature was removed. The periodontitis-induced rats showed a 149% increase in 8-hydroxydeoxyguanosine level and a 40% decrease in reduced:oxidized glutathione ratio in gingival tissue. Vitamin C intake induced a 175% increase in plasma vitamin C level, resulting in an improvement in the gingival 8-hydroxydeoxyguanosine level (decreased) and in the reduced:oxidized glutathione ratio (increased). Furthermore, in ligature-induced periodontitis lesions, gene expression encoding inflammation, including interleukin-1 alpha and interleukin-1 beta, was more than twofold down-regulated by vitamin C intake. The results suggest that systemic administration of vitamin C could be clinically beneficial in improving periodontitis-induced oxidative stress by down-regulating inflammatory gene expression. (C) 2008 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.freeradbiomed.2008.09.040

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大学生の口腔健康状態の調査および歯周健康状態と関連要因の検討.

古田美智子, 江國大輔, 入江浩一郎, 小山玲子, 三部俊博, 山中玲子, アクター ラヘナ, 山本龍生, 馬越通弘, 粕山健太, 森田 学

口腔衛生学会雑誌   59 ( 3 )   165 - 172   2009年

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

本研究の目的は、大学生の歯科疾患の有病状況の調査と、歯周健康状態と関連要因の検討をすることとした。分析対象者は、18〜28歳の大学新入生2,422名で、歯科健診とアンケートを実施した。現在歯数は28(22-32)[中央値(範囲)]、未処置歯保有者率は13.6%であった。歯周ポケットの深さ(pocket depth[PD])が4mm以上の部位を少なくとも1ヶ所有する者の割合は、4.9%であった。アンケートより、顎関節部の痛みがある者は8.0%、顎関節部の音を自覚する者は26.5%、開口障害がある者は8.9%であった。口内炎が繰り返しできる者は18.7%であった。また、未処置歯数、歯周健康状態、顎関節症の症状の有無には性差が認められた。歯周健康状態と関連要因を検討したところ、定期歯科受診、歯肉出血、歯肉腫脹、歯周病の自覚において、4mm以上の歯周ポケット保有者との関連が認められた。(著者抄録)

DOI： 10.5834/jdh.59.3_165

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2009&ichushi_jid=J00440&link_issn=&doc_id=20090804330002&doc_link_id=%2Fdk1dehea%2F2009%2F005903%2F003%2F0165-0172%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdk1dehea%2F2009%2F005903%2F003%2F0165-0172%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

歯周疾患対策として液体歯磨剤へ抗菌剤，抗炎症剤およびビタミン類を配合する意義 －ラットの実験的歯周炎を用いての組織学的・免疫組織化学的検討－

入江浩一郎, 友藤孝明, 江國大輔, 東 哲司, 三部俊博, 粕山健太, 馬越通弘, 山本龍生, 森田 学

日本歯周病学会会誌   2009年

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DOI： 10.2329/perio.51.252

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Cocoa-enriched diet decreases gingival oxidative stress in rat periodontitis

K. Irie, T. Tomofuji, D. Ekuni, T. Azuma, N. Tamaki, Y. Endo, T. Sanbe, T. Yamamoto, M. Morita

FREE RADICAL RESEARCH   43   76 - 76   2009年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：TAYLOR & FRANCIS LTD  

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Oxidative damage of rat liver induced by ligature-induced periodontitis and chronic ethanol consumption

Takaaki Tomofuji, Toshihiro Sanbe, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Takayuki Maruyama, Naofumi Tamaki, Tatsuo Yamamoto

ARCHIVES OF ORAL BIOLOGY   53 ( 12 )   1113 - 1118   2008年12月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: oxidative stress plays a central role in the initiation and progression of liver disease. Chronic ethanol consumption induces oxidative damage of the liver. Using a rat model, we previously showed that chronic administration of lipopolysaccharide and proteases to gingival sulcus induced both periodontal inflammation and liver injury. Periodontitis and ethanol consumption may have an additional effect on hepatic oxidative damage. The present study investigated the effects of periodontitis on ethanol-induced oxidative damage of the liver using a rat model.
Design: Male Wistar rats were divided into four groups (six rats/group). During the experimental period of eight weeks, two groups were fed an ethanol-containing liquid diet, and two groups were on a pair-fed control diet. Four weeks prior to the end of the experimental period, one group from each dietary treatment was ligated to induce periodontitis, while the other group was left unligated. In order to evaluate hepatic oxidative damage, the level of hexanoyl-lysine and the ratio of reduced form glutathione/oxidized form glutathione (GSH/GSSG) was determined. The concentration of blood hexanoyl-lysine was also measured as an index of circulating lipid peroxide.
Results: Ligature-induced periodontitis increased plasma levels of hexanoyl-lysine. in the liver, periodontitis decreased the CSH/GSSG ratio, and the combination of periodontitis and ethanol consumption induced a significant increase in hexanoyl-lysine level compared to ethanol consumption alone.
Conclusion: In the rat model, ligature-induced periodontitis increased plasma lipid peroxide, decreased the hepatic GSH/GSSG ratio and augmented ethanol-induced lipid peroxidation in the liver. (C) 2008 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2008.05.015

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Oxidative damage of rat liver induced by ligature-induced periodontitis and chronic ethanol consumption

Takaaki Tomofuji, Toshihiro Sanbe, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Takayuki Maruyama, Naofumi Tamaki, Tatsuo Yamamoto

ARCHIVES OF ORAL BIOLOGY   53 ( 12 )   1113 - 1118   2008年12月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: oxidative stress plays a central role in the initiation and progression of liver disease. Chronic ethanol consumption induces oxidative damage of the liver. Using a rat model, we previously showed that chronic administration of lipopolysaccharide and proteases to gingival sulcus induced both periodontal inflammation and liver injury. Periodontitis and ethanol consumption may have an additional effect on hepatic oxidative damage. The present study investigated the effects of periodontitis on ethanol-induced oxidative damage of the liver using a rat model.
Design: Male Wistar rats were divided into four groups (six rats/group). During the experimental period of eight weeks, two groups were fed an ethanol-containing liquid diet, and two groups were on a pair-fed control diet. Four weeks prior to the end of the experimental period, one group from each dietary treatment was ligated to induce periodontitis, while the other group was left unligated. In order to evaluate hepatic oxidative damage, the level of hexanoyl-lysine and the ratio of reduced form glutathione/oxidized form glutathione (GSH/GSSG) was determined. The concentration of blood hexanoyl-lysine was also measured as an index of circulating lipid peroxide.
Results: Ligature-induced periodontitis increased plasma levels of hexanoyl-lysine. in the liver, periodontitis decreased the CSH/GSSG ratio, and the combination of periodontitis and ethanol consumption induced a significant increase in hexanoyl-lysine level compared to ethanol consumption alone.
Conclusion: In the rat model, ligature-induced periodontitis increased plasma lipid peroxide, decreased the hepatic GSH/GSSG ratio and augmented ethanol-induced lipid peroxidation in the liver. (C) 2008 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2008.05.015

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Relationship Between Periodontal Condition and Plasma Reactive Oxygen Metabolites in Patients in the Maintenance Phase of Periodontal Treatment

Naofumi Tamaki, Takaaki Tomofuji, Takayuki Maruyama, Daisuke Ekuni, Reiko Yamanaka, Noriko Takeuchi, Tatsuo Yamamoto

JOURNAL OF PERIODONTOLOGY   79 ( 11 )   2136 - 2142   2008年11月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: The relationship between systemic antioxidative status and periodontal condition has been investigated in epidemiologic studies. However, little literature is available with regard to the correlation between systemic reactive oxygen species and periodontal condition. The purpose of this cross-sectional study was to investigate the relationship between plasma reactive oxygen metabolites (ROM) and periodontal condition in patients in the maintenance phase of periodontal treatment.
Methods: Eighty-one subjects (mean age: 57.4 years) who had entered a periodontal maintenance program were examined for probing depth (PD) and clinical attachment level (CAL). Plasma levels of ROM and biologic antioxidant potential (BAP) were determined with a free radical electric evaluator.
Results: The plasma level of ROM was positively correlated to mean CAL (r = 0.281; P = 0.011) and percentage of teeth with CAL A mm (r = 0.236; P = 0.034), but not mean PD (r = 0.196; P = 0.080). Logistic regression analysis showed that subjects with ROM levels &gt;400 Carratelli units (CARR U) had significantly higher mean CAL compared to subjects with ROM &gt;400 CARR U after adjusting for age, gender, and the number of teeth present (P = 0.011). However, the plasma level of BAP was not significantly correlated with the periodontal parameters.
Conclusions: A positive association was found between plasma oxidative status and CAL in patients in the maintenance phase of periodontal therapy. A systemic increase in oxidative stress may influence the rate of progression of periodontal disease. J Periodontol 2008; 79:2136-2142.

DOI： 10.1902/jop.2008.080082

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Relationship Between Periodontal Condition and Plasma Reactive Oxygen Metabolites in Patients in the Maintenance Phase of Periodontal Treatment 国際誌

Naofumi Tamaki, Takaaki Tomofuji, Takayuki Maruyama, Daisuke Ekuni, Reiko Yamanaka, Noriko Takeuchi, Tatsuo Yamamoto

JOURNAL OF PERIODONTOLOGY   79 ( 11 )   2136 - 2142   2008年11月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: The relationship between systemic antioxidative status and periodontal condition has been investigated in epidemiologic studies. However, little literature is available with regard to the correlation between systemic reactive oxygen species and periodontal condition. The purpose of this cross-sectional study was to investigate the relationship between plasma reactive oxygen metabolites (ROM) and periodontal condition in patients in the maintenance phase of periodontal treatment.
Methods: Eighty-one subjects (mean age: 57.4 years) who had entered a periodontal maintenance program were examined for probing depth (PD) and clinical attachment level (CAL). Plasma levels of ROM and biologic antioxidant potential (BAP) were determined with a free radical electric evaluator.
Results: The plasma level of ROM was positively correlated to mean CAL (r = 0.281; P = 0.011) and percentage of teeth with CAL A mm (r = 0.236; P = 0.034), but not mean PD (r = 0.196; P = 0.080). Logistic regression analysis showed that subjects with ROM levels &gt;400 Carratelli units (CARR U) had significantly higher mean CAL compared to subjects with ROM &gt;400 CARR U after adjusting for age, gender, and the number of teeth present (P = 0.011). However, the plasma level of BAP was not significantly correlated with the periodontal parameters.
Conclusions: A positive association was found between plasma oxidative status and CAL in patients in the maintenance phase of periodontal therapy. A systemic increase in oxidative stress may influence the rate of progression of periodontal disease. J Periodontol 2008; 79:2136-2142.

DOI： 10.1902/jop.2008.080082

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アンケートによる歯周疾患スクリーニングの有効性

小山 玲子, 玉木 直文, 丸山 貴之, 友藤 孝明, 江國 大輔, 山中 玲子, 東 哲司, 山本 龍生

口腔衛生学会雑誌   58 ( 5 )   557 - 557   2008年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯周炎モデルラットにおける歯肉酸化ストレス度・抗酸化力に対するビタミンC全身投与の効果

入江 浩一郎, 友藤 孝明, 江國 大輔, 三部 俊博, 東 哲司, 丸山 貴之, 玉木 直文, 山本 龍生, 森田 学

口腔衛生学会雑誌   58 ( 4 )   454 - 454   2008年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Relationship between body mass index and periodontitis in young Japanese adults

D. Ekuni, T. Yamamoto, R. Koyama, M. Tsuneishi, K. Naito, K. Tobe

JOURNAL OF PERIODONTAL RESEARCH   43 ( 4 )   417 - 421   2008年8月

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記述言語：英語   出版者・発行元：WILEY  

Background and Objective: Obesity has been implicated as a risk factor for several chronic health conditions. Recent studies have reported a relationship between obesity and periodontitis, but few studies have investigated this relationship in adolescents. The purpose of the present study was to investigate the relationship between body composition (i.e. body mass index and body fat) and periodontitis in university students in Japan.
Material and methods: Medical and oral health data were collected in a cross-sectional examination conducted by the Health and Environment Center of Okayama University. Students aged 18-24 years (n = 618), who were interested in receiving an oral health examination, were included in the analysis. The community periodontal index was used to assess periodontal status. Subjects with a community periodontal index score of 0-2 were considered as controls and those with a community periodontal index score of &gt; 2 were considered to have periodontitis. Logistic regression analysis was used to estimate the association between body mass index and periodontitis.
Results: The body mass index of all subjects was &lt; 30 kg/m(2). Age and body mass index were significantly associated with the community periodontal index. Logistic regression analysis revealed a 16% increased risk for periodontitis per 1-kg/m(2) increase in body mass index (adjusted odds ratio, 1.16; 95% confidence interval, 1.03-1.31; p &lt; 0.05).
Conclusion: Body mass index could be a potential risk factor for periodontitis among healthy young individuals (i.e. those with a body mass index of &lt; 30 kg/m(2)). It may be useful to include an evaluation of body mass index on a regular basis in university general and oral health examinations.

DOI： 10.1111/j.1600-0765.2007.01063.x

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Relationship between body mass index and periodontitis in young Japanese adults

D. Ekuni, T. Yamamoto, R. Koyama, M. Tsuneishi, K. Naito, K. Tobe

JOURNAL OF PERIODONTAL RESEARCH   43 ( 4 )   417 - 421   2008年8月

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記述言語：英語   出版者・発行元：WILEY  

Background and Objective: Obesity has been implicated as a risk factor for several chronic health conditions. Recent studies have reported a relationship between obesity and periodontitis, but few studies have investigated this relationship in adolescents. The purpose of the present study was to investigate the relationship between body composition (i.e. body mass index and body fat) and periodontitis in university students in Japan.
Material and methods: Medical and oral health data were collected in a cross-sectional examination conducted by the Health and Environment Center of Okayama University. Students aged 18-24 years (n = 618), who were interested in receiving an oral health examination, were included in the analysis. The community periodontal index was used to assess periodontal status. Subjects with a community periodontal index score of 0-2 were considered as controls and those with a community periodontal index score of &gt; 2 were considered to have periodontitis. Logistic regression analysis was used to estimate the association between body mass index and periodontitis.
Results: The body mass index of all subjects was &lt; 30 kg/m(2). Age and body mass index were significantly associated with the community periodontal index. Logistic regression analysis revealed a 16% increased risk for periodontitis per 1-kg/m(2) increase in body mass index (adjusted odds ratio, 1.16; 95% confidence interval, 1.03-1.31; p &lt; 0.05).
Conclusion: Body mass index could be a potential risk factor for periodontitis among healthy young individuals (i.e. those with a body mass index of &lt; 30 kg/m(2)). It may be useful to include an evaluation of body mass index on a regular basis in university general and oral health examinations.

DOI： 10.1111/j.1600-0765.2007.01063.x

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Effects of ethanol consumption on periodontal inflammation in rats

K. Irie, T. Tomofuji, N. Tamaki, T. Sanbe, D. Ekuni, T. Azuma, T. Maruyama, T. Yamamoto

JOURNAL OF DENTAL RESEARCH   87 ( 5 )   456 - 460   2008年5月

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記述言語：英語   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

Studies suggest a correlation between ethanol consumption and periodontal disease. We hypothesized that elevated levels of blood reactive oxygen species following ethanol consumption may increase inflammation in periodontal tissue. Rats were divided into 4 groups (6-7 rats/group). Two groups were fed an ethanol-containing liquid diet, and 2 groups were fed a pair-fed control diet. In one of each dietary group, periodontitis was ligature-induced, while the other group was left unligated. Chronic ethanol feeding alone decreased the ratio of reduced/oxidized glutathione and increased 8-hydroxydeoxy guanosine and tumor necrosis factor (TNF)-alpha levels in the gingiva. Blood hydroperoxides were also increased. In ligature-induced periodontitis lesions, ethanol feeding enhanced polymorphonuclear leukocyte infiltration and TNF-alpha expression. The results suggest that chronic alcohol consumption increased periodontal inflammation, oxidative damage, and TNF-alpha production and had an additive effect on polymorphonuclear leukocyte infiltration and gingival oxidative damage, increasing the severity of periodontal inflammation in the ligature model.

DOI： 10.1177/154405910808700511

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Effects of ethanol consumption on periodontal inflammation in rats

K. Irie, T. Tomofuji, N. Tamaki, T. Sanbe, D. Ekuni, T. Azuma, T. Maruyama, T. Yamamoto

JOURNAL OF DENTAL RESEARCH   87 ( 5 )   456 - 460   2008年5月

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記述言語：英語   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

Studies suggest a correlation between ethanol consumption and periodontal disease. We hypothesized that elevated levels of blood reactive oxygen species following ethanol consumption may increase inflammation in periodontal tissue. Rats were divided into 4 groups (6-7 rats/group). Two groups were fed an ethanol-containing liquid diet, and 2 groups were fed a pair-fed control diet. In one of each dietary group, periodontitis was ligature-induced, while the other group was left unligated. Chronic ethanol feeding alone decreased the ratio of reduced/oxidized glutathione and increased 8-hydroxydeoxy guanosine and tumor necrosis factor (TNF)-alpha levels in the gingiva. Blood hydroperoxides were also increased. In ligature-induced periodontitis lesions, ethanol feeding enhanced polymorphonuclear leukocyte infiltration and TNF-alpha expression. The results suggest that chronic alcohol consumption increased periodontal inflammation, oxidative damage, and TNF-alpha production and had an additive effect on polymorphonuclear leukocyte infiltration and gingival oxidative damage, increasing the severity of periodontal inflammation in the ligature model.

DOI： 10.1177/154405910808700511

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歯周組織の状態と血漿の酸化ストレス度・抗酸化力との関係

丸山 貴之, 玉木 直文, 友藤 孝明, 江國 大輔, 山中 玲子, 竹内 倫子, 山本 龍生

日本歯周病学会会誌   50 ( 春季特別 )   137 - 137   2008年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis 国際誌

Daisuke Ekuni, Takaaki Tomofuji, Naofumi Tamaki, Toshihiro Sanbe, Tetsuji Azuma, Reiko Yamanaka, Tatsuo Yamamoto, Tatsuo Watanabe

ARCHIVES OF ORAL BIOLOGY   53 ( 4 )   324 - 329   2008年4月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: Gingival cells respond to periodontal pathogens by generating reactive oxygen species, and such a condition would increase circulating oxidative stress. Improvement of gingival inflammation by toothbrushing may offer clinical benefits on not only periodontal health but also the circulatory conditions. We examined the effects of mechanical stimulation on the plasma 8-hydroxydeoxyguanosine level in a rat periodontitis model.
Design: In this experiment, male Wistar rats (n = 18) were divided into three groups. The control group received topical application of pyrogen-free water to the gingival sulcus for 8 weeks, while the other two groups received topical application of bacterial pathogens (lipopolysaccharide and proteases). After 4 weeks, half of the rats in the experimental groups received daily mechanical stimulation with an electric toothbrush for 4 weeks.
Results: Rats treated with bacterial pathogens presented periodontal tissue damage and increased plasma levels of 8-hydroxydeoxyguanosine. Mechanical stimulation by toothbrushing decreased gingival inflammation and oxidative DNA damage indicated by a decrease in plasma 8-hydroxydeoxyguanosine.
Conclusions: Mechanical stimulation of periodontally involved gingiva reduced 8-hydroxydeoxyguanosine in plasma and may contribute to a reduction in circulating oxidative stress associated molecules. (C) 2007 Elsevier Ltd. All rights reserved.

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Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis 国際誌

Daisuke Ekuni, Takaaki Tomofuji, Naofumi Tamaki, Toshihiro Sanbe, Tetsuji Azuma, Reiko Yamanaka, Tatsuo Yamamoto, Tatsuo Watanabe

ARCHIVES OF ORAL BIOLOGY   53 ( 4 )   324 - 329   2008年4月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: Gingival cells respond to periodontal pathogens by generating reactive oxygen species, and such a condition would increase circulating oxidative stress. Improvement of gingival inflammation by toothbrushing may offer clinical benefits on not only periodontal health but also the circulatory conditions. We examined the effects of mechanical stimulation on the plasma 8-hydroxydeoxyguanosine level in a rat periodontitis model.
Design: In this experiment, male Wistar rats (n = 18) were divided into three groups. The control group received topical application of pyrogen-free water to the gingival sulcus for 8 weeks, while the other two groups received topical application of bacterial pathogens (lipopolysaccharide and proteases). After 4 weeks, half of the rats in the experimental groups received daily mechanical stimulation with an electric toothbrush for 4 weeks.
Results: Rats treated with bacterial pathogens presented periodontal tissue damage and increased plasma levels of 8-hydroxydeoxyguanosine. Mechanical stimulation by toothbrushing decreased gingival inflammation and oxidative DNA damage indicated by a decrease in plasma 8-hydroxydeoxyguanosine.
Conclusions: Mechanical stimulation of periodontally involved gingiva reduced 8-hydroxydeoxyguanosine in plasma and may contribute to a reduction in circulating oxidative stress associated molecules. (C) 2007 Elsevier Ltd. All rights reserved.

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岡山大学医学部・歯学部附属病院の頭頚部癌チーム医療における予防歯科の関わり

山中玲子, 水川展吉, 山本龍生, 小山玲子, 古田美智子, 江國大輔, 西川悟郎, 岡崎恵子, 志茂加代子, 羽川 操, 森田 学

岡山歯学会雑誌   27 ( 2 )   93 - 98   2008年

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岡山大学医学部・歯学部附属病院の頭頚部癌チーム医療における予防歯科の関わり

山中玲子, 水川展吉, 山本龍生, 小山玲子, 古田美智子, 江國大輔, 西川悟郎, 岡崎恵子, 志茂加代子, 羽川操, 森田学

岡山歯学会雑誌   27 ( 2 )   93 - 98   2008年

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Oral administration of vitamin c prevents alveolar bone resorption induced by high dietary cholesterol in rats 国際誌

Toshihiro Sanbe, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto

JOURNAL OF PERIODONTOLOGY   78 ( 11 )   2165 - 2170   2007年11月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: A high-cholesterol diet stimulates alveolar bone resorption, which may be induced via tissue oxidative damage. Vitamin C reduces tissue oxidative damage by neutralizing free radicals and scavenging hydroxyl radicals, and its antioxidant effect may offer the clinical benefit of preventing alveolar bone resorption in cases of hyperlipidernia. We examined whether vitamin C could suppress alveolar bone resorption in rats fed a high-cholesterol diet.
Methods: In this 12-week study, rats were divided into four groups: a control group (fed a regular diet) and three experimental groups (fed a high-cholesterol diet supplemented with 0, 1, or 2 g/l vitamin C). Vitamin C was provided by adding it to the drinking water. The bone mineral density of the alveolar bone was analyzed by microcomputerized tomography. As an index of tissue oxidative damage, the 8-hydroxydeoxyguanosine level in the periodontal tissue was determined using a competitive enzyme-linked immunosorbent assay.
Results: Hyperlipidemia, induced by a high-cholesterol diet, decreased rat alveolar bone density and increased the number of tartrate-resistant acid phosphatase-positive osteoclasts. The expression of 8-hydroxydeoxyguanosine was upregulated in the periodontal tissues. Intake of vitamin C reduced the effect of a high-cholesterol diet on alveolar bone density and osteoclast differentiation and decreased periodontal 8-hydroxydeoxyguanosine expression.
Conclusion: In the rat model, vitamin C suppressed alveolar bone resorption, induced by high dietary cholesterol, by decreasing the oxidative damage of periodontal tissue.

DOI： 10.1902/jop.2007.070181

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Oral administration of vitamin c prevents alveolar bone resorption induced by high dietary cholesterol in rats 国際誌

Toshihiro Sanbe, Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Naofumi Tamaki, Tatsuo Yamamoto

JOURNAL OF PERIODONTOLOGY   78 ( 11 )   2165 - 2170   2007年11月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: A high-cholesterol diet stimulates alveolar bone resorption, which may be induced via tissue oxidative damage. Vitamin C reduces tissue oxidative damage by neutralizing free radicals and scavenging hydroxyl radicals, and its antioxidant effect may offer the clinical benefit of preventing alveolar bone resorption in cases of hyperlipidernia. We examined whether vitamin C could suppress alveolar bone resorption in rats fed a high-cholesterol diet.
Methods: In this 12-week study, rats were divided into four groups: a control group (fed a regular diet) and three experimental groups (fed a high-cholesterol diet supplemented with 0, 1, or 2 g/l vitamin C). Vitamin C was provided by adding it to the drinking water. The bone mineral density of the alveolar bone was analyzed by microcomputerized tomography. As an index of tissue oxidative damage, the 8-hydroxydeoxyguanosine level in the periodontal tissue was determined using a competitive enzyme-linked immunosorbent assay.
Results: Hyperlipidemia, induced by a high-cholesterol diet, decreased rat alveolar bone density and increased the number of tartrate-resistant acid phosphatase-positive osteoclasts. The expression of 8-hydroxydeoxyguanosine was upregulated in the periodontal tissues. Intake of vitamin C reduced the effect of a high-cholesterol diet on alveolar bone density and osteoclast differentiation and decreased periodontal 8-hydroxydeoxyguanosine expression.
Conclusion: In the rat model, vitamin C suppressed alveolar bone resorption, induced by high dietary cholesterol, by decreasing the oxidative damage of periodontal tissue.

DOI： 10.1902/jop.2007.070181

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Chronic administration of lipopolysaccharide and proteases induces periodontal inflammation and hepatic steatosis in rats 国際誌

Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Hiroki Kusano, Tetsuji Azuma, Toshihiro Sanbe, Naofumi Tamaki, Tatsuo Yamamoto, Tatsuo Watanabe, Mutsumi Miyauchi, Takashi Takata

JOURNAL OF PERIODONTOLOGY   78 ( 10 )   1999 - 2006   2007年10月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Epidemiologic studies suggest a relationship between periodontitis and liver diseases. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and liver diseases.
Methods: Fourteen male Wistar rats (8 weeks old) were divided into two groups: a periodontitis group in which Escherichia coli lipopolysaccharide (LPS) and Streptomyces griseus proteases were applied into the gingival sulcus for 8 weeks, and a control group using pyrogen-free water instead. After blood samples were collected, periodontal tissues and liver specimens were analyzed.
Results: Chronic administration of LPS and proteases to the gingival sulcus induced periodontitis and liver injury, including steatosis with inflammation and sinusoidal fibrosis. Apoptosis, enhanced concentration of 8-hydroxydeoxyguanosine, and activated production of tumor necrosis factor-alpha in liver were observed in the periodontitis group, with increased gingival inflammation, serum LPS, and reactive oxygen species.
Conclusion: Periodontal inflammation in a rat model induced fatty liver disease through increased serum LPS.

DOI： 10.1902/jop.2007.070056

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Chronic administration of lipopolysaccharide and proteases induces periodontal inflammation and hepatic steatosis in rats 国際誌

Takaaki Tomofuji, Daisuke Ekuni, Reiko Yamanaka, Hiroki Kusano, Tetsuji Azuma, Toshihiro Sanbe, Naofumi Tamaki, Tatsuo Yamamoto, Tatsuo Watanabe, Mutsumi Miyauchi, Takashi Takata

JOURNAL OF PERIODONTOLOGY   78 ( 10 )   1999 - 2006   2007年10月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Epidemiologic studies suggest a relationship between periodontitis and liver diseases. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and liver diseases.
Methods: Fourteen male Wistar rats (8 weeks old) were divided into two groups: a periodontitis group in which Escherichia coli lipopolysaccharide (LPS) and Streptomyces griseus proteases were applied into the gingival sulcus for 8 weeks, and a control group using pyrogen-free water instead. After blood samples were collected, periodontal tissues and liver specimens were analyzed.
Results: Chronic administration of LPS and proteases to the gingival sulcus induced periodontitis and liver injury, including steatosis with inflammation and sinusoidal fibrosis. Apoptosis, enhanced concentration of 8-hydroxydeoxyguanosine, and activated production of tumor necrosis factor-alpha in liver were observed in the periodontitis group, with increased gingival inflammation, serum LPS, and reactive oxygen species.
Conclusion: Periodontal inflammation in a rat model induced fatty liver disease through increased serum LPS.

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歯周疾患と脂肪肝 (特集 医科-歯科診療の連携) -- (口腔疾患・口腔機能と内科疾患)

山本 龍生, 江國 大輔, 友藤 孝明

綜合臨床   56 ( 10 )   2872 - 2876   2007年10月

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記述言語：日本語   出版者・発行元：永井書店  

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その他リンク： http://search.jamas.or.jp/link/ui/2008081266

ラット歯周炎モデルにおける肝病変に及ぼすブラッシングの影響

三部 俊博, 友藤 孝明, 江國 大輔, 山中 怜子, 東 哲司, 玉木 直文, 山本 龍生, 渡邊 達夫, 宮内 睦美, 高田 隆

日本歯周病学会会誌   49 ( 春季特別 )   177 - 177   2007年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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ラット歯肉細胞の細胞増殖活性に対する通電刺激の影響

東 哲司, 友藤 孝明, 江國 大輔, 三部 俊博, 山本 龍生, 渡邊 達夫, 鼻戸 由美

日本歯周病学会会誌   49 ( 春季特別 )   163 - 163   2007年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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ラット歯槽骨の骨密度に及ぼす高コレステロール食摂取の影響

友藤 孝明, 江國 大輔, 東 哲司, 三部 俊博, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   49 ( 春季特別 )   174 - 174   2007年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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Location of proliferating gingival cells following toothbrushing stimulation

T. Tomofuji, T. Sakamoto, D. Ekuni, T. Yamamoto, T. Watanabe

ORAL DISEASES   13 ( 1 )   77 - 81   2007年1月

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記述言語：英語   出版者・発行元：BLACKWELL PUBLISHING  

OBJECTIVES: Mechanical stimulation by toothbrushing promotes healing of gingivitis through accelerating cell proliferation. Junctional epithelium proliferates at periodontal pocket formation. A question is arisen whether toothbrushing contributes to the repair of gingival inflammation or deterioration of pocket formation. The location of proliferating cells in gingiva stimulated mechanically by toothbrushing was investigated.
MATERIALS AND METHODS: A total of 24 teeth of dogs underwent daily plaque removal with a curette (plaque removal) or both plaque removal and toothbrushing (toothbrushing). Proliferative activity of gingival cells in six individual zones was evaluated by assaying expression of proliferating cell nuclear antigen (PCNA).
RESULTS: Toothbrushing increased densities of PCNA-positive basal cells in the junctional epithelium, connective tissues adjacent to the junctional epithelium, the alveolar bone of the oral epithelial side and the oral epithelium. However, the densities of PCNA-positive cells at the apical portion of the junctional epithelium, connective tissues adjacent to the cementum and the alveolar bone of the periodontal ligament side did not increase following toothbrushing.
CONCLUSIONS: Toothbrushing promotes proliferation of gingival cells other than fibroblasts in periodontium and basal cells in the apical portion of the junctional epithelium. The repair of periodontal tissues might be promoted by toothbrushing within the limit of the direct mechanical stimulation.

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Location of proliferating gingival cells following toothbrushing stimulation

T. Tomofuji, T. Sakamoto, D. Ekuni, T. Yamamoto, T. Watanabe

ORAL DISEASES   13 ( 1 )   77 - 81   2007年1月

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記述言語：英語   出版者・発行元：BLACKWELL PUBLISHING  

OBJECTIVES: Mechanical stimulation by toothbrushing promotes healing of gingivitis through accelerating cell proliferation. Junctional epithelium proliferates at periodontal pocket formation. A question is arisen whether toothbrushing contributes to the repair of gingival inflammation or deterioration of pocket formation. The location of proliferating cells in gingiva stimulated mechanically by toothbrushing was investigated.
MATERIALS AND METHODS: A total of 24 teeth of dogs underwent daily plaque removal with a curette (plaque removal) or both plaque removal and toothbrushing (toothbrushing). Proliferative activity of gingival cells in six individual zones was evaluated by assaying expression of proliferating cell nuclear antigen (PCNA).
RESULTS: Toothbrushing increased densities of PCNA-positive basal cells in the junctional epithelium, connective tissues adjacent to the junctional epithelium, the alveolar bone of the oral epithelial side and the oral epithelium. However, the densities of PCNA-positive cells at the apical portion of the junctional epithelium, connective tissues adjacent to the cementum and the alveolar bone of the periodontal ligament side did not increase following toothbrushing.
CONCLUSIONS: Toothbrushing promotes proliferation of gingival cells other than fibroblasts in periodontium and basal cells in the apical portion of the junctional epithelium. The repair of periodontal tissues might be promoted by toothbrushing within the limit of the direct mechanical stimulation.

DOI： 10.1111/j.1601-0825.2006.01250.x

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歯周疾患と脂肪肝

山本龍生, 江國大輔, 友藤孝明

総合臨牀   56 ( 10 )   2827 - 2876   2007年

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ラット歯周炎モデルに対するアミノ酸配合歯磨剤の効果

三部 俊博, 草野 弘揮, 江國 大輔, 東 哲司, 山中 玲子, 友藤 孝明, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH   56 ( 4 )   453 - 453   2006年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Regulation of epithelial cell growth factor receptor protein and gene expression using a rat periodontitis model

D. Ekuni, J. D. Firth, E. E. Putnins

JOURNAL OF PERIODONTAL RESEARCH   41 ( 4 )   340 - 349   2006年8月

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記述言語：英語   出版者・発行元：BLACKWELL PUBLISHING  

Background and Objective: Regulation of epithelial cell behavior associated with periodontitis is not well elucidated but many responses will ultimately be regulated by growth factor receptors. Using a rat experimental periodontitis model, protein and gene expression of select growth factor receptors in junctional and pocket epithelium were examined.
Material and Methods: Periodontal disease was induced by daily topical application of lipopolysaccharide using an established protocol. Animals were killed at time 0 (control), and at 2 and 8 wk. Frozen tissue samples were collected from the right palatal gingival soft tissue, and the left periodontal tissues were decalcified and embedded in paraffin. Laser microdissection and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to quantify keratinocyte growth factor receptor (KGFR), hepatocyte growth factor receptor (HGFR), epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor 1 (FGFR1) gene expression, and in situ RT-PCR localized these increases to specific epithelial cells. Receptor protein expression was examined immunohistochemically. In cell culture, induction of HGFR and KGFR protein expression by serum, lipopolysaccharide and pro-inflammatory cytokines were examined using flow cytometry.
Results: Eight-week tissue samples exhibited histological changes consistent with periodontitis. KGFR and HGFR gene and protein expression were significantly induced at the 8 wk time point. KGFR expression was significantly up-regulated in basal and parabasal pocket epithelial cells, but HGFR was up-regulated throughout the pocket epithelium. In cell culture serum, lipopolysaccharide and pro-inflammatory cytokines, interleukin-1 beta and tumour necrosis factor-alpha significantly induced KGFR protein receptor expression, but HGFR expression was only induced by serum.
Conclusion: KGFR and HGFR are highly up-regulated in this model of periodontal disease and may play a significant role in regulating the proliferation and migration of pocket epithelium.

DOI： 10.1111/j.1600-0765.2006.00881.x

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Oxidative damage of periodontal tissue in the rat periodontitis model: Effects of a high-cholesterol diet 国際誌

T Tomofuji, T Azuma, H Kusano, T Sanbe, D Ekuni, N Tamaki, T Yamamoto, T Watanabe

FEBS LETTERS   580 ( 15 )   3601 - 3604   2006年6月

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記述言語：英語   出版者・発行元：ELSEVIER SCIENCE BV  

Studies suggest an association between consumption of a high-cholesterol diet and periodontitis. We addressed the mechanism by which high dietary cholesterol could be detrimental to periodontal health in a rat model. Feeding a high-cholesterol diet augmented the effects of bacterial pathogens and their products (e.g., lipopolysaccharide and proteases) on production of pro-inflammatory cytokines in fibroblasts. High dietary cholesterol also increased mitochondrial 8-hydroxydeoxyguanosine in the periodontal tissues. These results suggest that excessive tissue oxidative damage induced by high dietary cholesterol could potentiate pro-inflammatory cytokine production by fibroblasts stimulated with bacterial pathogens. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

DOI： 10.1016/j.febslet.2006.05.041

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ラット歯周炎モデルのTNF-αとIL-1βの産生に対する高コレステロール食の影響

東 哲司, 友藤 孝明, 草野 弘揮, 江國 大輔, 山本 龍生, 渡邊 達夫

岡山歯学会雑誌   25 ( 1 )   9 - 10   2006年6月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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高コレステロール食はIL-1βやTNF-α経路と独立してRANKL発現により骨吸収を促進する

江國 大輔, 友藤 孝明, 山中 玲子, 草野 弘揮, 東 哲司, 三部 俊博, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   48 ( 春季特別 )   187 - 187   2006年3月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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ラット歯周炎モデルにおける血中 Lipopolysaccharide 濃度に及ぼすブラッシングの影響

東 哲司, 友藤 孝明, 江國 大輔, 山中 玲子, 草野 弘揮, 三部 俊博, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   48 ( 春季特別 )   175 - 175   2006年3月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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RNA integrity and in situ RT-PCR in dento-alveolar tissues after microwave accelerated demineralisation 国際誌

D Ekuni, JD Firth, EE Putnins

ARCHIVES OF ORAL BIOLOGY   51 ( 2 )   164 - 169   2006年2月

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記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

Objective: The structural organization of oral soft tissue and its relationship with highly calcified teeth are difficult to preserve unless tissues are decalcified, paraffin embedded and subsequently sectioned. However, enamel decalcification time and its negative impact on RNA integrity makes it difficult to effectively analyse in situ gene expression. This study examined the impact of microwave-enhanced decalcification on processing time, RNA integrity and detection of in situ mRNA expression in hard and soft tissue for cell type specific markers of Keratinocyte growth factor receptor, Scleraxis and Osteonectin.
Design: Maxillas and mandibles were obtained from three mate Wistar strain rats. Right side tissues were decalcified using a microwave plus 10% EDTA solution (M+) while left side tissues were decalcified in 10% EDTA solution alone (M-).
Results: Microwave use reduced decalcification time by up to 50% and had no significant impact on morphology, RNA quality and in situ detection of gene expression relative to the M-group.
Conclusions: In situ RT-PCR gene expression of microwave decalcified paraffin-embedded oral tissues is an effective technique to localize in situ gene expression while maintaining excellent soft and hard tissue architecture. (c) 2005 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.archoralbio.2005.06.010

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Spatial extent of proliferation of oral sulcular epithelium by toothbrushing

Sakamoto T, Horiuchi M, Tomofuji T, Ekuni D, Yamamoto T, Watanabe T

Int J Oral Health   2006年

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ラット歯周炎モデルに対するビタミンC配合歯磨剤の効果

友藤孝明, 草野弘揮, 東 哲司, 三部俊博, 山中玲子, 江國大輔, 山本龍生, 渡邊達夫

日本歯周病学会会誌   48 ( 3 )   201 - 207   2006年

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

ビタミンCの経口摂取が歯周炎に有効であることが疫学研究により報告されている。ビタミンCによる歯周炎の組織変化を知ることは、歯周炎の予防・治療を考える上で有用である。本研究では、ビタミンC配合歯磨剤の効果を、ラット歯周炎モデルを用いて組織学的に検討した。8週齢のラット50匹を用いた。ラットの上顎第一臼歯の歯肉溝に、1日1回、歯周炎誘発物質(リポポリサッカライドとプロテアーゼ)を塗布し、歯周炎を惹起した。塗布開始4週後にラットを5群に分け、歯周炎誘発物質に加えて0%(基材のみ:歯周炎群)、1%、2%、3%または5%のビタミンCを配合した歯磨剤を3週間塗布した。その結果、3%と5%のビタミンC配合群は歯周炎群よりも、結合組織の好中球数が有意に減少し、コラーゲン密度が著明に増加した。また、歯周組織における8-ヒドロキシデオキシグアノシン(酸化ストレスの指標)の発現は、歯周炎群よりもビタミンC配合群において有意に低かった。これらの結果から、3%以上のビタミンCを配合した歯磨剤は、歯周炎組織において好中球浸潤を軽減し、コラーゲン合成を促すことで炎症を軽減させること、また、1%以上のビタミンC配合歯磨剤は歯周炎における酸化ストレスを低下させることが示唆された。(著者抄録)

DOI： 10.2329/perio.48.201

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Effects of a high-cholesterol diet on cell behavior in rat periodontitis

T Tomofuji, H Kusano, T Azuma, D Ekuni, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH   84 ( 8 )   752 - 756   2005年8月

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記述言語：英語   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

Studies have shown an association between periodontitis and serum cholesterol levels. We hypothesized that high dietary cholesterol could influence periodontitis as a result of proliferation of the junctional epithelium. Rats were divided into 4 groups. Two groups were fed a regular diet, and 2 groups were fed a high-cholesterol diet. One of each dietary group was treated with periodontitis-inducing agents ( lipopolysaccharide and proteases), while the other was treated with pyrogen-free water. Feeding rats with a high-cholesterol diet induced an increase in blood total cholesterol and a decrease in high-density lipoprotein cholesterol. Proliferation of the junctional epithelium with increasing bone resorption was promoted by the consumption of a high-cholesterol diet. High dietary cholesterol further increased the cell-proliferative activity of the junctional epithelium induced by lipopolysaccharide and proteases. These results suggest that high dietary cholesterol can initiate and augment periodontitis in the rat periodontitis model.

DOI： 10.1177/154405910508400813

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高コレステロール食がラット付着上皮の根尖側移動に与える影響

草野 弘揮, 友藤 孝明, 東 哲司, 江國 大輔, 山本 龍生, 渡邊 達夫

岡山歯学会雑誌   24 ( 1 )   11 - 11   2005年6月

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記述言語：日本語   出版者・発行元：岡山歯学会  

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歯肉溝へのLipopolysaccharideおよびプロテアーゼ投与によるラット肝組織の変化

友藤 孝明, 東 哲司, 草野 弘揮, 江國 大輔, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   47 ( 春季特別 )   82 - 82   2005年3月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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Initial apical migration of junctional epithelium in rats following application of lipopolysaccharide and proteases 国際誌

D Ekuni, T Tomofuji, R Yamanaka, K Tachibana, T Yamamoto, T Watanabe

JOURNAL OF PERIODONTOLOGY   76 ( 1 )   43 - 48   2005年1月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Apical migration of junctional epithelium (JE) occurs in association with periodontal pocket formation. The aim of this study was to investigate the gingival changes occurring during apical migration of the JE following application of factors associated with inflammatory periodontal disease pathogenesis.
Methods: Six-week-old male Wistar rats were divided into six groups: three experimental groups to investigate gingival changes following 2, 4, and 8 weeks topical application of lipopolysaccharide (LPS) and proteases and three control groups using pyrogen-free water. After 2, 4 or 8 weeks, nuclear DNA fragmentation was detected in periodontal ligament (PDL) fibroblasts using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method, and proliferative activities of the basal cells and fibroblasts were evaluated through expression of proliferating cell nuclear antigen (PCNA). Collagen destruction was examined histologically.
Results: Gingiva treated with LPS and proteases showed an increase in PCNA-positive basal cells but not the fibroblasts. Collagen destruction was observed at 2 weeks; apical migration of the JE and TUNEL-positive fibroblasts was seen at 4 weeks.
Conclusions: Following application of LPS and proteases to rat gingival sulci, the apical migration of the JE appears to occur simultaneously with the apoptosis of PDL fibroblasts, which in turn follows proliferation of the basal cells and collagen destruction.

DOI： 10.1902/jop.2005.76.1.43

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Fibroblast proliferation and resultant increase in gingival tissue volume in rats fed a high-cholesterol diet.

Tomofuji T, Azuma T, Kusano H, Ekuni D, Sanbe T, Yamamoto T, Watanabe T

Int J Oral Health   2005年

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Beating stimulation promotes proliferative activity in rat gingival cells.

Ekuni D, Yamamoto T, Yamanaka R, Tomofuji T, Watanabe T

Dentistry in Japan   2005年

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ラット歯周炎モデルに対するグリチルリチン酸ジカリウム、クレイツイオンを配合した歯磨剤と機械的刺激との効果．

山本龍生, 江國大輔, 山中玲子, 坂本友紀, 林 浩範, 渡邊達夫

口腔衛生学会雑誌   55 ( 5 )   537 - 542   2005年

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記述言語：日本語   出版者・発行元：有限責任中間法人日本口腔衛生学会  

ラットの歯周炎に対する, グリチルリチン酸ジカリウム, クレイツイオン^[○!R]ハイパワー, クレイツイオン^[○!R]ナンバーワンを配合した歯磨剤の効果, および歯磨剤と機械的刺激を併用したときの効果を検討した.ラット40匹の両側上顎第一臼歯口蓋側歯肉溝に細菌由来のリポポリサッカライドとプロテアーゼを4週間塗布し, 歯周炎を惹起した.その後, それらに加えて4種類の歯磨剤(対照歯磨剤, グリチルリチン酸ジカリウム配合歯磨剤, グリチルリチン酸ジカリウムおよびクレイツイオン^[○!R]ハイパワー配合歯磨剤, グリチルリチン酸ジカリウムおよびクレイツイオン^[○!R]ナンバーワン配合歯磨剤)を4週間塗布し, 対照歯磨剤群以外のラットの左側第一臼歯口蓋側歯肉を電動歯ブラシで1日1回機械的に刺激した.屠殺後, 歯肉のヘマトキシリン・エオジン染色標本を作製し, 組織学的に比較検討した.その結果, グリチルリチン酸ジカリウムにクレイツイオン^[○!R]ハイパワーまたはクレイツイオン^[○!R]ナンバーワンを配合した歯磨剤は, それぞれ結合組織中または接合上皮内の好中球を有意に減少させた.また, グリチルリチン酸ジカリウム配合歯磨剤塗布に歯肉への機械的刺激を加えると, グリチルリチン酸ジカリウムにクレイツイオン^[○!R]ハイパワーまたはクレイツイオン^[○!R]ナンバーワンを配合した歯磨剤と同程度の結合組織中または接合上皮内の好中球を減少させる効果が得られた.

DOI： 10.5834/jdh.55.5_537

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その他リンク： http://search.jamas.or.jp/link/ui/2006032082

Gingival cell proliferation induced by use of a sonic toothbrush with warmed silicone rubber bristles 国際誌

T Tomofuji, H Kusano, T Azuma, D Ekuni, T Yamamoto, T Watanabe, T Kishimoto

JOURNAL OF PERIODONTOLOGY   75 ( 12 )   1636 - 1639   2004年12月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Toothbrushing promotes gingival cell proliferation, which may occur as the result of the physical stimulation of the gingiva. The present study evaluated the effects of temperature and silicone rubber bristles of a sonic toothbrush on gingival cell proliferation in dogs.
Methods: During the 5-week experimental period, one quadrant in each of eight dogs received a different toothbrushing regimen: a manual toothbrush or a sonic toothbrush with 1) nylon, 2) silicone rubber, or 3) warmed silicone rubber bristles. The proliferative activity of gingival cells was evaluated based on expression of proliferating cell nuclear antigen (PCNA).
Results: Use of the sonic toothbrushes produced a higher density of PCNA-positive and total fibroblasts than did use of a manual toothbrush. The warm silicone rubber bristles resulted in a higher density of PCNA-positive fibroblasts compared with the cooler silicone rubber bristle. The number of PCNA-positive basal cells in the junctional epithelium also increased following electric toothbrushing with warmed silicone rubber bristles.
Conclusions: The sonic toothbrush with silicone rubber bristles induced gingival fibroblast proliferation to a greater degree than a manual toothbrush. Warming the silicone rubber bristles increased their stimulatory effects on the proliferative activity of gingival cells.

DOI： 10.1902/jop.2004.75.12.1636

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予熱した電動歯ブラシによる歯肉細胞の増殖

草野 弘揮, 東 哲司, 友藤 孝明, 江國 大輔, 坂本 友紀, 山本 龍生, 渡邊 達夫, 岸本 隆

口腔衛生学会雑誌   54 ( 5 )   586 - 586   2004年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯肉線維芽細胞の増殖能とコラーゲン合成に及ぼす超音波と振動の影響

東 哲司, 草野 弘揮, 江國 大輔, 坂本 友紀, 友藤 孝明, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   46 ( 秋季特別 )   140 - 140   2004年9月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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ラット歯周病モデルにおける高コレステロール食の影響

友藤 孝明, 東 哲司, 草野 弘揮, 江國 大輔, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   46 ( 秋季特別 )   111 - 111   2004年9月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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ラット歯周病モデルにおいて機械的刺激は歯周炎の治癒を促進する

山中 玲子, 江國 大輔, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌 = JOURNAL OF DENTAL HEALTH   54 ( 4 )   324 - 324   2004年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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ラット歯周病モデルにおける機械的刺激の影響

山本 龍生, 江國 大輔, 山中 玲子, 渡邊 達夫

日本歯周病学会会誌   46 ( 春季特別 )   123 - 123   2004年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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機械的刺激によるラット歯肉の接合上皮基底細胞と線維芽細胞の細胞周期の変化

山中 玲子, 江國 大輔, 草野 弘輝, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   46 ( 春季特別 )   157 - 157   2004年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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Effects of toothbrushing frequency on proliferation of gingival cells and collagen synthesis 国際誌

T Yamamoto, T Tomofuji, D Ekuni, T Sakamoto, M Horiuchi, T Watanabe

JOURNAL OF CLINICAL PERIODONTOLOGY   31 ( 1 )   40 - 44   2004年1月

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

Background: Mechanical stimulation by toothbrushing enhances proliferation of fibroblasts and junctional epithelium (JE). These changes in gingiva may depend on the interval between toothbrushing. The effects of toothbrushing frequency on proliferation of gingival fibroblasts and basal cells of JE were evaluated.
Methods: Twelve mongrel dogs were used. Each tooth was brushed for 20 s at 1.96 N. The subepithelial connective tissue of JE was examined for proliferating cell nuclear antigen (PCNA)-positive fibroblasts and procollagen type-I C-peptide (PIP)-positive fibroblasts. JE was examined for PCNA-positive basal cells.
Results: Gingiva that received brushing twice a day showed increases in the density of fibroblasts and ratio of PCNA-positive fibroblasts to total fibroblasts at 4 weeks. The ratio of PIP-positive fibroblasts increased at 8 weeks in gingiva brushed twice a day and once a day. PCNA-positive basal cell ratio increased at 4 weeks in gingiva brushed twice a day and once a day.
Conclusions: A high frequency of brushing was associated with increased numbers of PCNA-positive fibroblasts, PIP-positive fibroblasts and PCNA-positive basal cells. Gingival cell proliferation increased and reached a plateau earlier in gingiva brushed twice a day than in gingiva brushed once a day.

DOI： 10.1111/j.0303-6979.2004.00439.x

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Gingival cell responses to sonic or oscillanting/rotating electric toothbrushes.

Tomofuji T, Yamamoto T, Sakamoto T, Ekuni D, Watanabe T

Int J oral Health   2004年

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Proteases augment the effects of lipopolysaccharide in rat gingiva

D Ekuni, T Yamamoto, R Yamanaka, K Tachibana, T Watanabe

JOURNAL OF PERIODONTAL RESEARCH   38 ( 6 )   591 - 596   2003年12月

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記述言語：英語   出版者・発行元：BLACKWELL MUNKSGAARD  

Objectives: Lipopolysaccharide (LPS) and proteases have been implicated as important factors in the initiation and progression of human periodontal diseases. A single application of LPS or proteases is insufficient to induce periodontal pocket formation or periodontitis. The aim of the present study was to assess the combined effect of lipopolysaccharide and proteases on rat periodontal tissues, and create a periodontal disease model.
Materials and methods: Forty male Wistar rats were divided into four groups: combination group (treated with both LPS and proteases solutions); LPS group; proteases group; and control. Each solution was introduced daily into the palatal gingival sulcus of maxillary molars for 8 weeks. The tissues were evaluated histometrically and immunohistochemically.
Results: In the LPS group, elongation of rete ridge, apical migration of junctional epithelium (JE), increased numbers of B cells in connective tissue, and resorption of alveolar bone were observed. In the proteases group, the increase in the number of infiltrating polymorphonuclear leukocytes and blood vessels in the connective tissue was greater than that of the LPS group.
Conclusions: The effects of LPS on periodontal tissues differed from those of proteases. The addition of proteases augmented and increased the effects of LPS, which were apical migration, intraepithelial cleavage of JE, and increased B cell density. The lesions in the combination group resembled established lesions of human periodontitis, with the exception of the low density of plasma cells.

DOI： 10.1034/j.1600-0765.2003.00694.x

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Gingival cell proliferation by electric toothbrushes with various brush-heads.

T Azuma, H Kusano, T Tomofuji, D Ekuni, T Sakamoto, T Yamamoto, T Watanabe, T Kishimoto

JOURNAL OF DENTAL RESEARCH   82   423 - 423   2003年12月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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ツインペッカー^【○!R】による歯肉出血の改善

下野 順子, 江國 大輔, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   45 ( 秋季特別 )   163 - 163   2003年9月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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電動歯ブラシの植毛と歯周組織の応答

草野 弘揮, 東 哲司, 友藤 孝明, 江國 大輔, 坂本 友紀, 山本 龍生, 渡邊 達夫, 岸本 隆

日本歯周病学会会誌   45 ( 秋季特別 )   162 - 162   2003年9月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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LPS, プロテアーゼによる歯周ポケット形成時の接合上皮の増殖能亢進とコラーゲン破壊

江國 大輔, 山中 玲子, 橘 健太郎, 友藤 孝明, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌   53 ( 4 )   359 - 359   2003年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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LPS and proteases induce apoptosis in periodontal ligament fibroblasts, but not in cementoblasts.

D. Ekuni, R. Yamanaka, K. Tachibana, T. Yamamoto, T. Watanabe

JOURNAL OF DENTAL RESEARCH   82   B291 - B291   2003年6月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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Proliferative activity of epithelial cells and fibroblasts by mechanical stimulation.

R. Yamanaka, D. Ekuni, T. Tomofuji, T. Yamamoto, T. Watanabe

JOURNAL OF DENTAL RESEARCH   82   B356 - B356   2003年6月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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Spatial extent of gingival cell activation due to mechanical stimulation by toothbrushing 国際誌

T Sakamoto, M Horiuchi, T Tomofuji, D Ekuni, T Yamamoto, T Watanabe

JOURNAL OF PERIODONTOLOGY   74 ( 5 )   585 - 589   2003年5月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Mechanical stimulation by toothbrushing enhances gingival fibroblast proliferation and collagen synthesis, and reduces inflammatory cell infiltration. The aim of this study was to investigate the spatial extent of proliferation of fibroblasts and endothelial cells in dog gingiva in response to mechanical stimulation by toothbrushing.
Methods: All maxillary fourth premolars and mandibular first molars of 6 mongrel dogs were used. Dental plaque was removed with a curet. One of each pair of bilateral teeth (in the same jaw) was assigned to the brushing group, and the corresponding tooth (opposite side) was assigned to the control group. The Bass method was used to brush the limited mesial half of the tooth at 1.96 N for 20 seconds with a fitted plastic stent. Immediately before fixation of tissue, the surface of brushed gingiva was notched to indicate the borderline between the brushed and non-brushed areas. Histometrical analyses of the sections were performed using assays for proliferating cell nuclear antigen (PCNA) and von Willebrand factor.
Results: The numbers of fibroblasts and PCNA-positive fibroblasts in the subepithelial connective tissue adjacent to oral sulcular epithelium significantly increased in brushed gingiva, not only in the brushed area but also in the non-brushed area 0 to 0.5 mm from the notch. Increased numbers of vascular endothelial cells were observed only in the brushed area.
Conclusion: The effect of mechanical stimulation by toothbrushing on gingival cell proliferation was not observed more than 0.5 mm from the brushed area. These results indicate that effective activation of gingival cell proliferation requires mechanical stimulation of gingiva in all areas.

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Optimum force and duration of toothbrushing to enhance gingival fibroblast proliferation and procollagen type I synthesis in dogs 国際誌

T Tomofuji, D Ekuni, T Yamamoto, M Horiuchi, T Sakamoto, T Watanabe

JOURNAL OF PERIODONTOLOGY   74 ( 5 )   630 - 634   2003年5月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Toothbrushing enhances gingival fibroblast proliferation, which promotes wound healing. Optimum force and duration of toothbrushing for stimulation of fibroblast proliferation are key factors in maximizing effects of toothbrushing on periodontal wound healing. We therefore evaluated the effects of different durations and forces of toothbrushing on proliferative activity and procollagen synthesis of gingival fibroblasts.
Methods: Twelve dogs were used. In each dog, buccal gingivae of 12 teeth were examined for 3 weeks. Nine of these 12 teeth were each assigned to 1 of 9 different combinations of brushing force (0.98, 1.96, or 2.45 N) and duration (10, 20, or 40 seconds). The remaining 3 teeth received plaque removal without brushing, via a scaler.
Results: Force and duration of toothbrushing affected both proliferating cell nuclear antigen (PCNA)-positive and procollagen Type I C-peptide (PIP)-positive fibroblast ratios (P&lt;0.05). The highest ratio of PCNA-positive fibroblasts was produced by brushing at 1.96 N for 20 seconds. The highest ratio of PIP-positive fibroblasts was produced by brushing at 1.96 N for 10 seconds.
Conclusions: Toothbrushing at certain forces and durations enhanced the proliferative activity and procollagen synthesis of gingival fibroblasts. The toothbrushing duration that increased procollagen synthesis (10 seconds) was shorter than that which increased fibroblast proliferative activity (20 seconds).

DOI： 10.1902/jop.2003.74.5.630

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LPS塗布によるラット歯根膜線維芽細胞のアポトーシス

江國 大輔, 山中 玲子, 橘 健太郎, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   45 ( 春季特別 )   126 - 126   2003年4月

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反復加圧刺激による上皮細胞と線維芽細胞の増殖能の違い

山中 玲子, 江國 大輔, 友藤 孝明, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   45 ( 春季特別 )   116 - 116   2003年4月

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記述言語：日本語   出版者・発行元：(NPO)日本歯周病学会  

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The effect of duration and force of mechanical toothbrushing stimulation on proliferative activity of the junctional epithelium 国際誌

T Tomofuji, M Morita, M Horiuchi, T Sakamoto, D Ekuni, T Yamamoto, T Watanabe

JOURNAL OF PERIODONTOLOGY   73 ( 10 )   1149 - 1152   2002年10月

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記述言語：英語   出版者・発行元：AMER ACAD PERIODONTOLOGY  

Background: Gingival bleeding is frequently accompanied by an ulcerated epithelium, with repair depending on the proliferative activity of the epithelial cells. The present study examined the proliferative activity of the junctional epithelium (JE) under several different methods of toothbrushing stimulation.
Methods: Twelve dogs were used in this 3-week experiment. Prior to the experiment, all teeth underwent daily removal of plaque and calculus using a scaler. Teeth were then divided into quadrants: 9 teeth in 3 quadrants (test, 3 per quadrant) were mechanically stimulated by toothbrushing for various time intervals (10, 20, or 40 seconds) and at various forces (100, 200, or 250 gf). The 3 teeth in the fourth quadrant served as controls. The proliferative activity of the basal cells of the junctional epithelium was evaluated for expression of proliferating cell nuclear antigen (PCNA). The numbers of PCNA-positive basal cells and total basal cells were counted, and the width of the junctional epithelium was measured.
Results: Toothbrushing force significantly affected the PCNA-positive basal cell ratio (P&lt;0.05). The 200 g toothbrushing force produced the highest PCNA-positive basal cell ratio, which was significantly higher than that using the 100 g force (P &lt;0.05).
Conclusion: In junctional epithelium stimulated with a toothbrush, the PCNA-positive basal cell ratio is more sensitive to toothbrushing force than to duration.

DOI： 10.1902/jop.2002.73.10.1149

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ブラッシング頻度の違いによるイヌ歯肉上皮細胞の増殖能の変化

友藤 孝明, 江國 大輔, 山本 龍生, 堀内 正純, 坂本 友紀, 渡邊 達夫

口腔衛生学会雑誌   52 ( 5 )   723 - 724   2002年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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D-29 LPS, プロテアーゼ投与によるTUNEL陽性細胞の出現と接合上皮の深部増殖

江國 大輔, 山中 玲子, 橘 健太郎, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   44 ( 秋季特別 )   162 - 162   2002年9月

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本歯周病学会  

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ブラッシングによって応答するイヌ歯肉PCNA陽性細胞の分布

江國 大輔, 友藤 孝明, 坂本 友紀, 堀内 正純, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌   52 ( 4 )   552 - 553   2002年8月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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C-9-9 : 30 イヌ歯肉線維芽細胞の増殖に及ぼす音波ブラシと高速反復回転運動ブラシの効果の比較

友藤 孝明, 江國 大輔, 山本 龍生, 堀内 正純, 坂本 友紀, 渡邊 達夫

日本歯周病学会会誌   44 ( 春季特別 )   126 - 126   2002年3月

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本歯周病学会  

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Comparison of gingival fibroblast proliferation with two electric toothbrushes.

T Tomofuji, D Ekuni, T Sakamoto, N Horiuchi, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH   81   A374 - A374   2002年3月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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PCNA positive cells in apical migration of rat's junctional epithelium.

T Yamamoto, D Ekuni, R Yamanaka, K Tachibana, T Watanabe

JOURNAL OF DENTAL RESEARCH   81   A372 - A372   2002年3月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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Apical migration of junctional epithelium by lipopolysaccharide and protease.

D Ekuni, R Yamanaka, K Tachibana, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH   81   A206 - A206   2002年3月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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ナイロンに多元素鉱物粉状体を混合して形成したフィラメントを用いた歯ブラシのブラッシング効果.

坂本友紀, 友藤孝明, 堀内正純, 江國大輔, 森田 学, 山本龍生, 渡邊達夫

口腔衛生学会雑誌   2002年

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DOI： 10.5834/jdh.52.3_221

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歯周病原因子を投与することによってラットに歯周炎を誘発するモデルの研究

江國大輔

岡山歯学会雑誌   2002年

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ブラッシング頻度の違いによるイヌ歯肉線維芽細胞の活性化

友藤 孝明, 江國 大輔, 坂本 友紀, 堀内 正純, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌   51 ( 4 )   552 - 553   2001年9月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Reduction of inflammatory cell infiltration by mechanical stimulation of toothbrushing.

T Sakamoto, D Ekuni, T Tomofuji, M Horiuchi, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH   80 ( 4 )   1343 - 1343   2001年4月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

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B-15-11 : 40 ブラッシング頻度の違いによるイヌ歯肉線維芽細胞の活性の変化

友藤 孝明, 江國 大輔, 坂本 友紀, 堀内 正純, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   43 ( 春季特別 )   101 - 101   2001年3月

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本歯周病学会  

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ブラッシングによるイヌ歯肉組織の応答範囲

坂本 友紀, 友藤 孝明, 江國 大輔, 堀内 正純, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌   50 ( 5 )   834 - 834   2000年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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D-7 E.coli LPS連続投与によるラット歯肉付着上皮の細胞増殖能の変化

江國 大輔, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   42 ( 秋季特別 )   174 - 174   2000年9月

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本歯周病学会  

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ブラッシング力と時間の違いによるイヌ歯肉炎症性細菌浸潤の変化

友藤 孝明, 山本 龍生, 堀内 正純, 坂本 友紀, 江國 大輔, 渡邊 達夫

口腔衛生学会雑誌   50 ( 4 )   564 - 565   2000年9月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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Location of PCNA positive gingival cells by toothbrushing.

T Tomofuji, D Ekuni, T Sakamoto, M Horiuchi, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH   79 ( 5 )   1239 - 1239   2000年5月

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC DENTAL RESEARCH  

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B-24-15 : 30 ブラッシング力の違いによるイヌ歯肉組織の応答

友藤 孝明, 江國 大輔, 坂本 友紀, 堀内 正純, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   42 ( 春季特別 )   106 - 106   2000年4月

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本歯周病学会  

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Boundary of gingival cells activation by toothbrushing

T Sakamoto, T Tomofuji, D Ekuni, M Horiuchi, T Yamamoto, T Watanabe

JOURNAL OF DENTAL RESEARCH   79   576 - 576   2000年

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER ASSOC DENTAL RESEARCH  

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イヌ歯周組織の細胞増殖に及ぼすブラッシングの影響

堀内 正純, 友藤 孝明, 江國 大輔, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌   49 ( 5 )   825 - 825   1999年10月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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歯肉組織のブラッシングに対する応答範囲

坂本 友紀, 友藤 孝明, 江國 大輔, 堀内 正純, 山本 龍生, 渡邊 達夫

口腔衛生学会雑誌   49 ( 4 )   636 - 637   1999年9月

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記述言語：日本語   出版者・発行元：(一社)日本口腔衛生学会  

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A-2-9 : 10 ブラッシングによる歯肉組織の応答範囲

坂本 友紀, 江國 大輔, 友藤 孝明, 堀内 正純, 山本 龍生, 渡邊 達夫

日本歯周病学会会誌   41 ( 秋季特別 )   57 - 57   1999年9月

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本歯周病学会  

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