研究者詳細 - 舟橋　弘晃

MISC - 舟橋　弘晃

分割表示 369 件中 81 - 100 件目／全件表示 >>

老齢種雄豚精子希釈液へのカルニチン添加が精子ミトコンドリア活性に及ぼす影響

佐野光, 舟橋弘晃

日本畜産学会大会講演要旨 109th 2008年

揺動培養器を用いたブタ卵母細胞の体外成熟と単為発生後の初期発生

舟橋弘晃, 小池孝幸, 成瀬恵治

日本生殖医学会雑誌 53 ( 1/2 ) 44 - 44 2008年

哺乳動物卵母細胞の体外成熟の現況

舟橋弘晃

Journal of clinical embryologist 9 4 - 8 2007年6月

カルニチンがブタ卵母細胞の体外成熟に及ぼす影響

赤木友香, 舟橋弘晃

日本畜産学会大会講演要旨 108th 67 - 67 2007年

限定培地へのPyruvate添加がブタ卵母細胞の核・細胞質成熟に及ぼす影響

小池孝幸, 坂井涼子, 舟橋弘晃

日本畜産学会大会講演要旨 108th 67 - 67 2007年

グルコースおよびピルビン酸がブタ卵母細胞の核成熟およびグルタチオン濃度に及ぼす影響

小池孝幸, 舟橋弘晃

日本畜産学会大会講演要旨 107th 74 - 74 2007年

小中卵胞由来ブタ卵母細胞-卵丘細胞塊におけるグルコース6リン酸脱水素酵素活性の所在

舟橋弘晃, 池原あかり, 石田有希

日本畜産学会大会講演要旨 107th 74 - 74 2007年

Nucleosideが成熟過程のマウス卵母細胞内ミトコンドリア膜電位活性に及ぼす影響

藤井渉, 舟橋弘晃

Journal of reproduction and development 53 ( Supplement ) j177 - j177 2007年

豚凍結精液の人工授精で用いる融解液の違いが人工授精後の繁殖成績および豚凍結精子の先体反応に及ぼす影響

山口昇一郎, 舟橋弘晃, 村上徹哉, 伊東正吾

日本畜産学会大会講演要旨 106th 101 - 101 2006年

豚精液の深部注入器によるAI技術の現状海外および国内の事例から

舟橋弘晃

養豚の友 ( 444 ) 2006年

体細胞を導入したラット卵母細胞の活性化後の発生能

藤井渉, 舟橋弘晃

Journal of reproduction and development 52 ( Supplement ) j77 - j77 2006年

Select antioxidants improve the function of extended boar semen stored at 10 degrees C 国際誌

H Funahashi, T Sano

THERIOGENOLOGY 63 ( 6 ) 1605 - 1616 2005年4月

記述言語：英語出版者・発行元：ELSEVIER SCIENCE INC

The objective was to determine the effects of antioxidant addition to extender on viability, acrosome integrity and penetrability in vitro of boar spermatozoa preserved at 10 degrees C. Washed spennatozoa were resuspended at 1 x 10(8) cells/mL in modified Modena solution containing 20% (v/ v) boar seminal plasma and 5 mM antioxidant (glutathione, cysteine or hypotaurine). Control aliquots were the same suspension without added antioxidants. Sperm suspensions were then chilled to 10 degrees C with a computerized cooling program. Sperm viability after 7 and 14 d was higher in the presence of glutathione or cysteine, whereas hypotaurine did not improve the survival rate. Percentage of chlortetracycline (CTC) fluorescence pattern as intact live cells was higher in spermatozoa preserved with glutathione or cysteine at 7 and 14 d of preservation. When the preservation period was prolonged until 57 d, survival rate was higher with cysteine than controls. When spermatozoa were preserved with cysteine and then inseminated in an IVF system, penetration rate was not different until 15 d of preservation and higher than controls at 15-29 d, whereas no sows became pregnant after AI with spermatozoa preserved for 21-23 d. Therefore, glutathione and cysteine can improve the viability and functional status of boar spermatozoa during liquid preservation and boar spermatozoa penetrated in vitro even after preservation in the presence of cysteine at 10 degrees C for 29 d. (c) 2004 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.theriogenology.2004.06.016

豚人工授精における技術的課題と技術開発方向２．精液注入器の改良．

舟橋弘晃

畜産技術第599号(2005年4月号) pp13-18 ( 599 ) 2005年

プロテアーゼ阻害剤による体外成熟豚卵子の前処理が体外受精系での精子侵入に及ぼす影響

舟橋弘晃

日本畜産学会大会講演要旨 104th 129 - 129 2005年

Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine 国際誌

H Funahashi, R Romar

REPRODUCTION 128 ( 6 ) 789 - 800 2004年12月

記述言語：英語出版者・発行元：BIO SCIENTIFICA LTD

To reduce the incidence of polyspermic penetration, the effects of transient exposure of washed fresh spermatozoa to caffeine in a brief co-culture in vitro fertilization (IVF) system were examined. A pretreatment effect of spermatozoa with adenosine was also examined. When 5 mmol caffeine/l was supplemented during periods of co-culture and additional culture periods until 8 h after insemination, a shortened co-incubation period of gametes (30 denuded oocytes in 100 mul modified Medium 199-suspended spermatozoa at 2.5 x 10(5) sperm/ml) from 30 to 5 min increased the monospermy rate in total mature oocytes examined. The number of spermatozoa binding to the zona surface was significantly lower in oocytes co-cultured for 5 min (33.1 +/- 2.2) than 8 h (207.6 +/- 13.7). A limited exposure of gametes to 5 mmol caffeine/l only during a transient co-culture period for 5 or 30 min significantly reduced the mean number of sperm cells that penetrated into the oocyte. Transient exposure of spermatozoa to caffeine for only 5 min increased the percentage of capacitated cells but not acrosome-reacted cells as compared with a whole exposure treatment. Furthermore, preincubation of spermatozoa with 10 mumol adenosine/l for 0 min increased both the incidence of capacitated cells and the monospermy rate and consequently decreased the number of sperm cells that penetrated into the oocyte. In conclusion, these results have demonstrated that a new transient co-incubation IVF system, in which denuded oocytes are co-cultured with spermatozoa in medium containing caffeine for 5 to 30 min and then continuing the culture in caffeine-free medium, will reduce the incidence of polyspermic penetration. Preincubation of fresh spermatozoa with adenosine before the transient co-incubation IVF can also improve the monospermy rate. Furthermore, asynchrony in the morphology of sperm nuclei in polyspermic oocytes was reduced by the pretreatment with adenosine and a brief exposure to caffeine.

DOI： 10.1530/rep.1.00295

Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine 国際誌

H Funahashi, R Romar

REPRODUCTION 128 ( 6 ) 789 - 800 2004年12月

記述言語：英語出版者・発行元：BIO SCIENTIFICA LTD

DOI： 10.1530/rep.1.00295

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人工授精や体外受精に用いる豚精子受精能の人為制御システムの開発．

舟橋弘晃

平成15年度食肉に関する助成研究調査成果報告書(財団法人伊藤記念財団) Vol.22, p55-61. 2004年