研究者詳細 - 舟橋　弘晃

MISC - 舟橋　弘晃

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完全体外培養系で得た牛体外受精凍結卵の移植試験

青柳敬人, 武富敏郎, 舟橋弘晃

JA全農飼料畜産中央研究所試験研究報告 ( 20(1991) ) 1992年

牛における個体別体外受精卵の作出試験

青柳敬人, 舟橋弘晃, 武田哲男

JA全農飼料畜産中央研究所試験研究報告 ( 20(1991) ) 1992年

黄体ホルモン製剤利用による牛の反復過排卵処置に関する検討

青柳敬人, 武富敏郎, 板倉はつえ, 亀山賢次, 舟橋弘晃, 武田哲男, 鬼原辰夫

JA全農飼料畜産中央研究所試験研究報告 ( 20(1991) ) 1992年

First cleavage of enucleated rat eggs following transplantation of karyoplast removed from pronuclear eggs stored at 2 to 6℃ for various durations.(共著)

Theriogenology 36 ( 3 ) 411 - 417 1991年9月

1ST CLEAVAGE OF ENUCLEATED RAT EGGS FOLLOWING TRANSPLANTATION OF KARYOPLAST REMOVED FROM PRONUCLEAR EGGS STORED AT 2-DEGREES-C TO 6-DEGREES-C FOR VARIOUS DURATIONS

K NIWA, H FUNAHASHI, K OHMAE, M KATTOH

THERIOGENOLOGY 36 ( 3 ) 411 - 417 1991年9月

記述言語：英語出版者・発行元：BUTTERWORTH-HEINEMANN

Pronuclear rat eggs were cultured for 24 to 48 hours at 37-degrees-C after storage at 2 to 6-degrees-C for 0 to 216 hours in medium. Very high proportions (89 to 97%) of eggs cleaved to the two-cell stage after storage for 0 to 48 hours. The proportion, however, decreased rapidly in eggs stored for 72 hours (60%), and eggs stored for more than 120 hours cleaved poorly. However, when male and female pronuclei from stored eggs were transplanted into enucleated fresh eggs, 92 to 100% of the fused eggs with karyoplast stored for 0 to 144 hours cleaved. Although the cleavage rate was reduced to 50% when karyoplast from eggs stored for 168 hours was transplanted, this reduction was not significant. Complete loss of cleaving ability was observed in fused eggs with the karyoplast stored for 216 hours. These results clearly indicate that the pronuclei of rat eggs can be stored for a longer period than the cytoplasm at low temperatures (2 to 6-degrees-C).

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Developmental capacity of bovine oocytes collected from ovaries of individual heifers and fertilized in vitro.(共著)

Theriogenology 36 ( 3 ) 427 - 434 1991年9月

DEVELOPMENTAL CAPACITY OF BOVINE OOCYTES COLLECTED FROM OVARIES OF INDIVIDUAL HEIFERS AND FERTILIZED INVITRO 国際誌

H FUNAHASHI, Y AOYAGI, T TAKEDA, T ONIHARA

THERIOGENOLOGY 36 ( 3 ) 427 - 434 1991年9月

記述言語：英語出版者・発行元：BUTTERWORTH-HEINEMANN

Bovine follicular oocytes from individual heifers (n = 49) were separately matured, fertilized with frozen-thawed spermatozoa and cultured with cumulus cells. Although there were great variations in the number (mean +/- SD = 19.1 +/- 11.9) of oocytes collected from individual heifers and the percentages of the oocytes cleaved 48 hours after insemination (mean +/- SD = 69.5 +/- 18.4) and developed to the morula stage 7 days after insemination (mean +/- SD = 10.9 +/- 10.9), there were significant correlations between the numbers of oocytes collected and cleaved (the correlation coefficient: r = 0.9336) or developed to morula stage (r = 0.6633), indicating that oocytes from different heifers have the same developmental ability after in vitro fertilization. Ten morulae and 12 blastocysts which were obtained 7 and 8 days after insemination were transferred, one by one, to each uterine horn of 11 recipients. At Day 60 of pregnancy, 8 (80%) fetuses were identified in 4 (80%) of 5 recipients into which 10 embryos were transferred at Day -1 of synchrony. However, only 3 (25%) fetuses were identified in 2 (40%) of 6 recipients into which 12 embryos were transferred at Day 0 or +1 of synchrony.

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黄体ホルモン製剤を用いた黒毛和種牛の反復過剰排卵処理に関する検討.(共著)

北海道牛受精卵移植研究会会報 ( 10 ) 19 - 21 1991年

ラット前核期胚および桑実期胚の低温保存におよぼすショ糖の影響.(共著)

家畜繁殖学雑誌 37 ( 4 ) a21 1991年

ラットおよびマウスの初期はいにおける属間核移植試験

舟橋弘晃, 武田哲男, 丹羽こう二