研究者詳細 - 舟橋　弘晃

MISC - 舟橋　弘晃

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Co-culture with follicular shell pieces(FSP)increases the development al competence of pig oocytes matured in vitro.(共著)

Biology of Reproduction 54 ( Supplement 1 ) 100 - 100 1996年

Low salt maturation medium enhances the histone H1 kinase activity of porcine oocytes at the end of in vitro maturation.(共著)

Hiroaki FUNAHASHI, Todd T. STUMPF, Nam-Hyung KIM, Billy N. DAY

Journal of Reproduction and Development 42 ( 2 ) 109 - 115 1996年

The effect of NaCl in maturation media on histone H1 kinase activity of porcine oocytes was examined at the end of culture for maturation. Oocyte-cumulus complexes were cultured in modified Whitten's medium containing different concentrations of NaCl (44.50, 68.49, 92.40, 116.40 or 140.35 mM). The media were supplemented with 10% porcine follicular fluid and hormones for 20 h and then without hormonal supplements for an additional 20 h. At the end of culture, oocytes were sampled for morphological observation, for glutathione assay and for histone H1 kinase assay. At the end of culture, the incidence of oocytes at each meiotic stage was not different among groups. However, histone H1 kinase activity and glutathione content were higher (P < 0.01) in oocytes cultured in media with lower NaCl concentrations. Histone H1 kinase activity in the oocytes at the end of maturation culture was correlated with intracellular glutathione content (r = 0.899, P < 0.01). These data indicate that histone H1 kinase activity and glutathione content of porcine oocytes at the end of culture for maturation are reduced when a high salt medium is used for maturation.

DOI： 10.1262/jrd.42.109

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糖類が豚卵子への精子侵入におよぼす影響(共著)

宋学雄, 沢井健, 許東けい, 舟橋弘晃, 丹羽こう二

第89回日本繁殖生物学会講演要旨 89th 57 1996年

システインおよびシスチンが豚卵胞卵子の応性前核形成能に及ぼす影響(共著)

沢井健, 舟橋弘晃, 丹羽こう二

第89回日本繁殖生物学会講演要旨 89th 44 1996年

成長ホルモンが牛卵胞卵子の成熟分裂の進行速度におよぼす影響(共著)

伊賀浩輔, 舟橋弘晃, 丹羽こう二

第89回日本繁殖生物学会講演要旨 89th 41 1996年

Nuclear morphology of swine oocytes during follicular development following stimulation by eCG injection.(共著)

Biology of Reproduction 54 ( Supplement 1 ) 408 - 408 1996年

Exposure of swine oocyte-cumulus complexes to dibutyryl cyclic AMP for the first 20 hours of maturation increases the homogeneity of oocyte unclear maturation.(共著)

Congress Programme of the 13th International Congress on Animal Reproduction 2 10 - 4 1996年

In vitro penetration of pig oocytes commercially prepared frozen-thawed boar spermatozoa in a chemically semi-defined bicarbonatefree medium.(共著)

Theriogenology 45 ( 1 ) 265 1996年

Low salt maturation medium enhances the histone H1 kinase activity of porcine oocytes at the end of in vitro maturation.(共著)

Journal of Reproduction and Development 42 ( 2 ) 109 - 115 1996年

Factors affecting nuclear and cytoplasmic maturation in pig oocytes

P Coy, S Ruiz, N Ouhibi, H Funahashi, BN Day, RM Moor

BIOLOGY OF REPRODUCTION 54 ( Supplement 1 ) 412 - 412 1996年

Co-culture with follicular shell pieces (FSP) increases the developmental competence of pig oocytes matured in vitro.

LR Abeydeera, H Funahashi, TC Cantley, A Rieke, BN Day

BIOLOGY OF REPRODUCTION 54 ( Supplement 1 ) 100 - 100 1996年

Nuclear morphology of swine oocytes during follicular development following stimulation by eCG injection.

H Funahashi, H Tatemoto, TC Cantley, BN Day

BIOLOGY OF REPRODUCTION 54 ( Supplement 1 ) 408 - 408 1996年

Exposure of swine oocyte-cumulus complexes to dibutyryl cyclic AMP for the first 20 hours of maturation increases the homogeneity of oocyte unclear maturation.(共著)

Congress Programme of the 13th International Congress on Animal Reproduction 2 10 - 4 1996年

In vitro penetration of pig oocytes commercially prepared frozen-thawed boar spermatozoa in a chemically semi-defined bicarbonatefree medium.(共著)

Theriogenology 45 ( 1 ) 265 1996年

Gamma-glutamyltranspeptidase of spermatozoa may reduce oocyte glutathione content at sperm penetration(共著)

Hiroaki Funahashi, Zoltan Machaty, Randall S. Prather, Billy N. Day

Molecular Reproduction and Development 45 ( 4 ) 485 - 490 1996年

The presence of γ-glutamyl transpeptidase (GGT) in boar spermatozoa and the potential role of the GGT at sperm penetration were examined using in vitro matured porcine oocytes. In the first experiment, GGT of boar spermatozoa was examined using a histochemical stain. GGT was detected in the midpiece and the acrosome regions of boar spermatozoa. In the second experiment, porcine oocytes matured in vitro were injected with approximately 40 pl of 10 mM HEPES solution alone or HEPES containing 0.5 U/ml GGT or 1 mM guanosine 5'-O-(3'-thiotriphosphate) (GTP-γ-S; G-protein activator). When GGT was injected into oocytes, the incidence of oocytes activated (23.7 ± 1.4%) was not different (P > 0.05) from HEPES-injected controls (24.9 ± 1.3%) at 6 h after injection. Injected GTP-γ-S, however, activated 76.0 ± 5.3% of oocytes at 6 h after injection, but extrusion of the second polar body was very low (2.8 ± 4.8%). Total content of glutathione (GSH) and glutathione disulfide (GSSG) did not differ (P > 0.05) between GTP-γ-S injected oocytes (4.2 ± 0.7 pmol/oocyte) and noninjected oocytes (4.0 ± 0.1 pmol/oocyte) at 6 h after injection. However, the total content of GSH and GSSG was lower (P < 0.01) in GGT-injected oocytes (2.1 ± 0.2 pmol/oocyte) than HEPES-injected oocytes (3.4 ± 0.2 pmol/oocyte) at 6 h after injection. In the third experiment, in vitro matured porcine oocytes were injected with about 40 pl of 10 mM HEPES solution alone or HEPES containing 0.5 U/ml GGT and then inseminated. At 12 h after insemination, the incidence of male pronuclear formation was significantly lower in oocytes injected with GGT as compared with injected control oocytes. These results demonstrated that (1) GGT was present on the surface of spermatozoa, (2) total oocyte content of GSH and GSSG was decreased by microinjection of GGT but not by that of GTP- γ-S, and (3) male pronuclear formation was inhibited in GGT-injected oocytes. These results suggest that sperm GGT may be a limiting factor for male pronuclear formation in polyspermic oocytes.

DOI： 10.1002/(SICI)1098-2795(199612)45:4<485::AID-MRD11>3.0.CO;2-W

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Factors affecting development in vitro of borine and rat 1-cell embryos(共著)

Journal of Mammalian Ova Research 13 ( 2 ) 71 - 80 1996年

Factors Affecting Development in Vitro of Bovine and Rat 1-Cell Embryos

Kazuchika Miyoshi, Hiroaki Funahashi, Koji Niwa

Journal of Mammalian Ova Research 13 ( 2 ) 71 - 80 1996年

Factors affecting nuclear and cytoplasmic maturation in pig oocytes.(共著)

Biology of Reproduction 54 ( Supplement 1 ) 412 - 412 1996年

Pronuclear formation and intracellular glutathione content of in vitro matured porcine oocytes following in vitro fertilization and/or electrical activation.(共著)

Zygote 3 ( 3 ) 273 - 281 1995年8月

Pronuclear visibility, development and transgene expression in IVM/IVF-derived porcine embryos.(共著)

Theriogenology 44 ( 3 ) 391 - 401 1995年8月

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