研究者詳細 - 王　英正

MISC - 王　英正

分割表示 146 件中 61 - 80 件目／全件表示 >>

心臓超音波検査による心臓内幹細胞自家移植療法に伴う心筋ストレイン変化の機能解析

逢坂大樹, 石神修大, 奥山倫弘, 後藤拓哉, 大月審一, 佐野俊二, 王英正

日本心エコー図学会学術集会抄録集 26th 2015年

心臓超音波および心臓MRIを用いた心臓内幹細胞自家移植療法に伴う心筋ストレイン変化の解析

逢坂大樹, 石神修大, 後藤拓弥, 大月審一, 笠原真悟, 佐野俊二, 王英正

日本小児循環器学会雑誌 31 ( Supplement 1 ) 2015年

左心低形成症候群に対する心臓内幹細胞自家移植療法:第I相臨床試験(TICAP trial)の3年長期成績報告

石神修大, 樽井俊, 大月審一, 後藤拓弥, 奥山倫弘, 逢坂大樹, 栄徳隆祐, 新井禎彦, 笠原真悟, 佐野俊二, 王英正

日本小児循環器学会雑誌 31 ( Supplement 1 ) 2015年

単心室症におけるHeart Failure with Preserved Ejection Fractionに対する心臓シャント手術及び心臓内幹細胞自家移植療法の効果

逢坂大樹, 石神修大, 後藤拓弥, 大月審一, 笠原真悟, 佐野俊二, 王英正

日本小児循環器学会雑誌 31 ( Supplement 1 ) 2015年

左心低形成症候群に対する心筋幹細胞を用いた再生医療

佐野俊二, 樽井俊, 石神修大, 小林純子, 後藤拓弥, 奥山倫弘, 逢坂大樹, 黒子洋介, 馬場健児, 大月審一, 新井禎彦, 笠原真悟, 王英正

日本小児循環器学会雑誌 31 ( Supplement 1 ) 2015年

繊維化心筋を有する小児単心室症の心臓MRIによる機能解析と心筋幹細胞移植療法による繊維化抑制の可能性

石神修大, 後藤拓弥, 逢坂大樹, 奥山倫弘, 栄徳隆裕, 馬場健児, 大月審一, 笠原真悟, 佐野俊二, 王英正

日本小児循環器学会雑誌 31 ( Supplement 1 ) 2015年

小児心不全に対する心臓内自己幹細胞移植治療の標準医療化へ向けた取り組み

石神修大, 後藤拓弥, 奥山倫弘, 逢坂大樹, 新井禎彦, 笠原真悟, 大月審一, 佐野俊二, 王英正

日本小児循環器学会雑誌 31 ( Supplement 1 ) 2015年

小児心不全に対する心臓内幹細胞自家移植療法

後藤拓弥, 石神修大, 逢坂大樹, 佐野俊二, 王英正

日本小児血液・がん学会雑誌(Web) 52 ( 3 ) 2015年

Cardiac Magnetic Resonance Late Gadolinium Enhancement is Associated With Ventricular Elastance That May Predict Latent Ventricular Dysfunction After Fontan Procedure

Michihiro Okuyama, Shuta Ishigami, Daiki Ousaka, Junko Kobayashi, Sadahiko Arai, Shingo Kasahara, Shunji Sano, Hidemasa Oh

CIRCULATION 130 2014年11月

Intracoronary Delivery of Autologous Cardiac Progenitor Cells in Children With Hypoplastic Left Heart Syndrome: The Ticap Trial With 18-Month Follow Up

Shuta Ishigami, Suguru Tarui, Michihiro Okuyama, Daiki Ousaka, Shinichi Ohtsuki, Takahiro Eitoku, Junko Kobayashi, Shingo Kasahara, Shunji Sano, Hidemasa Oh

CIRCULATION 130 2014年11月

Disease-Specific Induced Pluripotent Stem Cells Identify the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 During Cardiac Development of Hypoplastic Left Heart Syndrome

Junko Kobayashi, Masashi Yoshida, Suguru Tarui, Shuta Ishigami, Michihiro Okuyama, Yusuke Nagai, Shingo Kasahara, Keiji Naruse, Hiroshi Ito, Shunji Sano, Hidemasa Oh

CIRCULATION 130 2014年11月

Directed Differentiation of Patient-Specific Induced Pluripotent Stem Cells Identifies the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 in Hypoplastic Left Heart Syndrome

Junko Kobayashi, Masashi Yoshida, Suguru Tarui, Masataka Hirata, Yusuke Nagai, Shingo Kasahara, Keiji Naruse, Hiroshi Ito, Shunji Sano, Hidemasa Oh

PLOS ONE 9 ( 7 ) e102796-e102796 2014年7月

記述言語：英語出版者・発行元：PUBLIC LIBRARY SCIENCE

The genetic basis of hypoplastic left heart syndrome (HLHS) remains unknown, and the lack of animal models to reconstitute the cardiac maldevelopment has hampered the study of this disease. This study investigated the altered control of transcriptional and epigenetic programs that may affect the development of HLHS by using disease-specific induced pluripotent stem (iPS) cells. Cardiac progenitor cells (CPCs) were isolated from patients with congenital heart diseases to generate patient-specific iPS cells. Comparative gene expression analysis of HLHS-and biventricle (BV) heart-derived iPS cells was performed to dissect the complex genetic circuits that may promote the disease phenotype. Both HLHS-and BV heart-derived CPCs were reprogrammed to generate disease-specific iPS cells, which showed characteristic human embryonic stem cell signatures, expressed pluripotency markers, and could give rise to cardiomyocytes. However, HLHS-iPS cells exhibited lower cardiomyogenic differentiation potential than BV-iPS cells. Quantitative gene expression analysis demonstrated that HLHS-derived iPS cells showed transcriptional repression of NKX2-5, reduced levels of TBX2 and NOTCH/HEY signaling, and inhibited HAND1/2 transcripts compared with control cells. Although both HLHS-derived CPCs and iPS cells showed reduced SRE and TNNT2 transcriptional activation compared with BV-derived cells, co-transfection of NKX2-5, HAND1, and NOTCH1 into HLHS-derived cells resulted in synergistic restoration of these promoters activation. Notably, gain- and loss-of-function studies revealed that NKX2-5 had a predominant impact on NPPA transcriptional activation. Moreover, differentiated HLHS-derived iPS cells showed reduced H3K4 dimethylation as well as histone H3 acetylation but increased H3K27 trimethylation to inhibit transcriptional activation on the NKX2-5 promoter. These findings suggest that patient-specific iPS cells may provide molecular insights into complex transcriptional and epigenetic mechanisms, at least in part, through combinatorial expression of NKX2-5, HAND1, and NOTCH1 that coordinately contribute to cardiac malformations in HLHS.

DOI： 10.1371/journal.pone.0102796

Late Gadolinium Enhancement on Cardiac MRI Identifies Ventricular Dysfunction and Regional Myocardial Dyssynchrony in Patients with Univentricular Heart Diseases

Michihiro Okuyama, Shuta Ishigami, Daiki Ousaka, Junko Kobayashi, Shingo Kasahara, Shunji Sano, Hidemasa Oh

CIRCULATION RESEARCH 115 2014年7月

Intracoronary Autologous Cardiac Progenitor Cell Transfer In Children With Hypoplastic Left Heart Syndrome: 2-year Results Of The Ticap Trial

Shuta Ishigami, Suguru Tarui, Michihiro Okuyama, Daiki Ousaka, Junko Kobayashi, Shingo Kasahara, Shunji Sano, Hidemasa Oh

CIRCULATION RESEARCH 115 2014年7月

MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric cardiac hypertrophy induced by α1-adrenergic receptors.

Ogata T, Naito D, Nakanishi N, Hayashi YK, Taniguchi T, Miyagawa K, Hamaoka T, Maruyama N, Matoba S, Ikeda K, Yamada H, Oh H, Ueyama T

Proc Natl Acad Sci U S A. 111 ( 10 ) 3811 - 3816 2014年3月

Stem cell therapies in patients with single ventricle physiology.

Methodist Debakey Cardiovascular Journal. 10 ( 2 ) 77 - 81 2014年

小児心不全の再生医療からのアプローチ

石神修大, 佐野俊二, 王英正

移植 50 ( 6 ) 54 - 63 2014年

先天性心疾患外科手術における再生医療

循環器内科 75 ( 1 ) 87 - 93 2014年

3D細胞外マトリックスを利用したヒト幹細胞再播種によるバイオ人工心臓の作成

奥山倫弘, 逢坂大樹, 石神修大, 小林純子, 笠原真悟, 新井禎彦, 佐野俊二, 王英正

再生医療 13 2014年

機能的単心室症に対する心臓内幹細胞自家移植療法:第I/II相臨床試験の2年目長期成績

石神修大, 樽井俊, 奥山倫弘, 逢坂大機, 大月審一, 栄徳隆祐, 小林純子, 笠原真悟, 佐野俊二, 王英正

日本小児循環器学会雑誌 30 ( Supplement ) 2014年

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