研究者詳細 - 髙橋　卓

論文 - 髙橋　卓

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Cloning and characterization of an L1 layer-specific gene in Arabidopsis thaliana

M Abe, T Takahashi, Y Komeda

PLANT AND CELL PHYSIOLOGY 40 ( 6 ) 571 - 580 1999年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

The shoot apical meristem unknown. In order to identify the genes that function in the SAM, we performed cDNA subtraction experiments between wild-type and terminal flower1 shoot apices. Here, we describe the cloning of a gene designated PDF1 (PROTODERMAL FACTOR1). In situ hybridization revealed that the expression of PDF1 is exclusively limited to the L1 layer of vegetative, inflorescence and floral meristems and to the protoderm of organ primordia. By contrast, P (SAM) of Arabidopsis thaliana constitutes the tunica of L1 and L2 and the corpus represented by L3 cells. Regulatory networks involved in establishing and maintaining this structure of shoot meristems remain largelyDF1 shows no detectable level of expression in the epidermis of mature organs. Specific expression of the PDF1 gene in protodermal cells is also observed during embryogenesis. The deduced amino acid sequence of PDF1 shares no significant homology with that of other known proteins but contains a putative signal peptide and novel proline-rich repeat motifs, suggesting a cell-wall protein. Possible roles of the PDF1 gene in the SAM are discussed.

Web of Science

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熱ショック応答の分子機構

高橋卓, 米田好文

蛋白質核酸酵素臨時増刊「環境応答・適応の分子機構」 44巻 39-44頁 1999年

The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia(共著)

Plant Journal 15 ( 3 ) 301 - 310 1998年8月

Identification by PCR of receptor-like protein kinases from Arabidopsis flowers. (共著)

Plant Molecular Biology 37 ( 4 ) 587 - 596 1998年7月

ACL5 : an Arabidopsis gene required for internodal elongation after flowering(共著)

Plant Journal 12 ( 4 ) 863 - 874 1997年

DIMINUTO gene of Arabidopsis is involved in regulating cell elongation(共著)

Takahashi T

Gene & Development 9 ( 1 ) 97 - 107 1995年1月

ANALYSIS OF TISSUE-SPECIFIC EXPRESSION OF ARABIDOPSIS-THALIANA HSP9O-FAMILY GENE HSP81 査読

N YABE, T TAKAHASHI, Y KOMEDA

PLANT AND CELL PHYSIOLOGY 35 ( 8 ) 1207 - 1219 1994年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE SOC PLANT PHYSIOLOGISTS

We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures. Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22 degrees C. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a beta-glucuronidase (GUS) gene, confirmed these results. At 22 degrees C, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35 degrees C treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants.
Exogenous application of various chemicals such as ABA, GA(3), kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22 degrees C; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.

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Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from Arabidopsis and a bacterial gene for β-glucuronidase(共著)

TAKAHASHI Taku, YABE Naoto, KOMEDA Yoshibumi

Plant and Cell Physiology 34 ( 1 ) 161 - 164 1993年

記述言語：英語出版者・発行元：Japanese Society of Plant Physiologists

Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

CiNii Article

CiNii Books

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=181806

The Arabidopsis HPS18.2 promoter/GUS gene fusion in transgenic Arabidopsis plants : a powerful tool for the isolation of regulatory mutants of the heat-shock response(共著)

Plant Journal 2 ( 5 ) 751 - 761 1992年

Isolation and analysis of the expression of two genes for the 81-kilodalton heat-shock proteins from Arabidopsis(共著)

Plant Physiology 99 ( 2 ) 383 - 390 1992年

Heat-shock induced accumulation of extremely long transcripts containing the rDNA intergenic spacer in Arabidopsis(共著)

Takahashi Taku, Komeda Yoshibumi

Plant and Cell Physiology 33 ( 4 ) 389 - 394 1992年

記述言語：英語出版者・発行元：Japanese Society of Plant Physiologists

A cDNA clone containing a 1.2-kb fragment derived from the ribosomal DNA (rDNA) intergenic spacer (IGS) of Arabidopsis was characterized. Isolation of this clone was achieved in a previous study by differential screening of a cDNA library prepared from heat-shocked Arabidopsis seedlings (Takahashi and Komeda 1989). Nucleotide sequence analysis revealed that the cDNA had an oligo(dA) sequence that corresponded to the sequence found in the IGS. Northern blot analysis using the cloned cDNA as probe demonstrated that heat-shock treatment of seedlings induced the accumulation of extremely long transcripts with apparent lengths of more than 7 kb. Treatment with arsenite and cadmium ions also led to the accumulation of the RNA, a phenomenon very similar to that observed in the case of expression of genes for heat-shock proteins (HSPs). Although the possibility remains to be excluded that these transcripts might be derived from,a region homologous to the IGS Iocated outside the rDNA clusters in Arabidopsis, it is most likely that processing of precursor rRNA and/or termination of transcription of rRNA are greatly impaired under stressed conditions in plants, as reported in the case of animal genes for rRNA (Labhart and Reeder 1987, Parker and Bond 1989).

CiNii Article

CiNii Books

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=181630

Characterization of two genes encoding small heat-shock proteins in Arabidopsis thaliana(共著)

Takahashi T, Komeda Y

Molecular and General Genetics 219 ( 3 ) 365 - 372 1989年11月

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