研究者詳細 - 髙橋　卓

論文 - 髙橋　卓

分割表示 92 件中 1 - 20 件目／全件表示 >>

Specific enhancement of the translation of thermospermine-responsive uORF-containing mRNAs by ribosomal mutations in Arabidopsis thaliana 査読

Koki Mutsuda, Yuichi Nishii, Tomohiko Toyoshima, Hiroko Fukushima, Hiroyasu Motose, Taku Takahashi

Plant Signaling & Behavior 2025年12月

Involvement of a NIMA-related kinase in cell division of the liverwort Marchantia polymorpha 査読

Hikari Mase, Aoi Sumiura, Yoshihiro Yoshitake, Takayuki Kohchi, Taku Takahashi, Hiroyasu Motose

Plant And Cell Physiology 2025年5月

ATML1 and PDF2 regulate cuticle formation and protect the plant body from environmental stresses in Arabidopsis thaliana seedlings 査読

Kenji Nagata, Ichiro Maekawa, Taku Takahashi, Mitsutomo Abe

Journal of Plant Research 2025年3月

Effect of environmental conditions on seed germination and seedling growth in Cuscuta campestris

Koki Nagao, Taku Takahashi, Ryusuke Yokoyama

2024年6月

Thermospermine Is an Evolutionarily Ancestral Phytohormone Required for Organ Development and Stress Responses in Marchantia Polymorpha. 査読

Takuya Furumoto, Shohei Yamaoka, Takayuki Kohchi, Hiroyasu Motose, Taku Takahashi

Plant & cell physiology 65 ( 3 ) 460 - 471 2024年4月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Thermospermine suppresses auxin-inducible xylem differentiation, whereas its structural isomer, spermine, is involved in stress responses in angiosperms. The thermospermine synthase, ACAULIS5 (ACL5), is conserved from algae to land plants, but its physiological functions remain elusive in non-vascular plants. Here, we focused on MpACL5, a gene in the liverwort Marchantia polymorpha, that rescued the dwarf phenotype of the acl5 mutant in Arabidopsis. In the Mpacl5 mutants generated by genome editing, severe growth retardation was observed in the vegetative organ, thallus, and the sexual reproductive organ, gametangiophore. The mutant gametangiophores exhibited remarkable morphological defects such as short stalks, fasciation and indeterminate growth. Two gametangiophores fused together, and new gametangiophores were often initiated from the old ones. Furthermore, Mpacl5 showed altered responses to heat and salt stresses. Given the absence of spermine in bryophytes, these results suggest that thermospermine has a dual primordial function in organ development and stress responses in M. polymorpha. The stress response function may have eventually been assigned to spermine during land plant evolution.

DOI： 10.1093/pcp/pcae002

PubMed

researchmap

Microtubule-associated proteins WDL5 and WDL6 play a critical role in pollen tube growth in Arabidopsis thaliana 査読

Takashi Okamoto, Hiroyasu Motose, Taku Takahashi

Plant Signaling & Behavior 18 ( 1 ) 2023年11月

Suppression of the dwarf phenotype of an Arabidopsis mutant defective in thermospermine biosynthesis by a synonymous codon change in the SAC51 uORF. 査読国際誌

Yuichi Nishii, Daiki Koyama, Hiroko Fukushima, Taku Takahashi

Molecular genetics and genomics : MGG 2023年10月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Thermospermine plays a critical role in negatively regulating xylem development in angiosperms. A mutant of Arabidopsis thaliana that is defective in thermospermine biosynthesis, acaulis5 (acl5), exhibits a dwarf phenotype with excessive xylem formation. Mechanistically thermospermine acts in attenuating the inhibitory effect of an evolutionarily conserved upstream open reading frame (uORF) on the main ORF of SAC51, which encodes a basic helix-loop-helix protein involved in xylem repression. Here, we revealed that a semidominant suppressor of acl5, sac503, which partially restores the acl5 phenotype, has a point mutation in the conserved uORF of SAC51 with no amino acid substitution in the deduced peptide sequence. In transgenic lines carrying the β-glucuronidase (GUS) reporter gene fused with the SAC51 5' region containing the uORF, the mutant construct was shown to confer higher GUS activity than does the wild-type SAC51 construct. We confirmed that sac503 mRNA was more stable than SAC51 mRNA in acl5. These results suggest that the single-base change in sac503 positively affects the translation of its main ORF instead of thermospermine. We further found that the uORF-GUS fusion protein could be synthesized in planta from the wild-type and sac503 translational fusion constructs.

DOI： 10.1007/s00438-023-02076-4

PubMed

researchmap

Loss of function of an Arabidopsis homologue of JMJD6 suppresses the dwarf phenotype of acl5, a mutant defective in thermospermine biosynthesis. 査読国際誌

Hirotoshi Matsuo, Hiroko Fukushima, Shinpei Kurokawa, Eri Kawano, Takashi Okamoto, Hiroyasu Motose, Taku Takahashi

FEBS letters 596 ( 23 ) 3005 - 3014 2022年12月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

In Arabidopsis thaliana, the ACL5 gene encodes thermospermine synthase and its mutant, acl5, exhibits a dwarf phenotype with excessive xylem formation. Studies of suppressor mutants of acl5 reveal the involvement of thermospermine in enhancing mRNA translation of the SAC51 gene family. We show here that a mutant, sac59, which partially suppresses the acl5 phenotype, has a point mutation in JMJ22 encoding a D6-class Jumonji C protein (JMJD6). A T-DNA insertion allele, jmj22-2, also partially suppressed the acl5 phenotype while mutants of its closest two homologs JMJ21 and JMJ20 had no such effects, suggesting a unique role for JMJ22 in plant development. We found that mRNAs of the SAC51 family are more stabilized in acl5 jmj22-2 than in acl5.

DOI： 10.1002/1873-3468.14470

PubMed

researchmap

RNA processing/modifying enzymes play key roles in the response to thermospermine in Arabidopsis thaliana

Takahiro Tanaka, Daiki Koyama, Mitsuru Saraumi, Hiroyasu Motose, Taku Takahashi

bioRxiv 2022年9月

担当区分：責任著者出版者・発行元：Cold Spring Harbor Laboratory

Abstract

Thermospermine acts in negative regulation of xylem differentiation through enhancing mRNA translation of the members of the SAC51 gene family in Arabidopsis. These mRNAs contain conserved upstream open-reading-frames (uORFs) that are inhibitory to the main ORF translation. To address the mode of action of thermospermine in this process, we have isolated mutants that are insensitive to thermospermine, named its. We show here that four genes responsible for the mutants, its1 to its4, encode a homologue of SPOUT RNA methyl transferase, an rRNA pseudouridine synthase CBF5/NAP57, a putative spliceosome disassembly factor STIPL1/NTR1, and a plant-specific RNA-binding protein PHIP1, respectively. While these mutants except its1 are almost normal in appearance, they enhance the dwarf phenotype of a mutant of ACL5 defective in thermospermine biosynthesis, resulting in tiny-sized plants reminiscent of a double knockout of ACL5 and SACL3, a member of the SAC51 family. We confirmed that the GUS reporter activity from the SAC51 5’-GUS fusion transcript was severely reduced in all of these mutants. These results unveil the importance of RNA processing and modification for the translation of transcripts containing regulatory uORFs.

DOI： 10.1101/2022.09.19.508594

researchmap

Establishment and application of novel culture methods in Marchantia polymorpha: persistent tip growth is required for substrate penetration by rhizoids 招待査読

Hikari Mase, Hirofumi Nakagami, Takashi Okamoto, Taku Takahashi, Hiroyasu Motose

Commun.Integr. Biol. 15 164 - 167 2022年

Plant specific armadillo repeat kinesin directs organelle transport and microtubule convergence to promote tip growth

Asaka Kanda, Kento Otani, Taku Takahashi, Hiroyasu Motose

bioRxiv 2022年

出版者・発行元：Cold Spring Harbor Laboratory

Abstract

Tip growth is essential for plant growth and reproduction. However, it remains elusive how highly polarized tip-growing cells coordinate the organization of intracellular components. Here, we show that plant-specific armadillo-repeat containing kinesin, MpARK, mediates organelle transport and microtubule convergence in tip growing rhizoids in the early diverging land plantMarchantia polymorpha. MpARK is required for anterograde transport of organelles to maintain their position. Furthermore, MpARK participates in the formation of microtubule foci at the rhizoid apex to stabilize growth direction. MpARK-dependent rhizoid growth is essential for plant anchorage and soil holding capacity. Thus, ARK might be a principal organelle transporter and intracellular organizer in the primitive rooting cells for the adaptation to terrestrial environments. Our findings suggest that ARK is functionally comparable to canonical anterograde kinesins in animals and fungi.

DOI： 10.1101/2022.07.08.499237

researchmap

Responses of Polyamine-Metabolic Genes to Polyamines and Plant Stress Hormones in Arabidopsis Seedlings 査読

Yusaku Yariuchi, Takashi Okamoto, Yoshiteru Noutoshi, Taku Takahashi

Cells 10 ( 12 ) 3283 - 3283 2021年11月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

In plants, many of the enzymes in polyamine metabolism are encoded by multiple genes, whose expressions are differentially regulated under different physiological conditions. For comprehensive understanding of their regulation during the seedling growth stage, we examined the expression of polyamine metabolic genes in response to polyamines and stress-related plant hormones in Arabidopsis thaliana. While confirming previous findings such as induction of many of the genes by abscisic acid, induction of arginase genes and a copper amine oxidase gene, CuAOα3, by methyl jasmonate, that of an arginine decarboxylase gene, ADC2, and a spermine synthase gene, SPMS, by salicylic acid, and negative feedback regulation of thermospermine biosynthetic genes by thermospermine, our results showed that expressions of most of the genes are not responsive to exogenous polyamines. We thus examined expression of OsPAO6, which encodes an apoplastic polyamine oxidase and is strongly induced by polyamines in rice, by using the promoter-GUS fusion in transgenic Arabidopsis seedlings. The GUS activity was increased by treatment with methyl jasmonate but neither by polyamines nor by other plant hormones, suggesting a difference in the response to polyamines between Arabidopsis and rice. Our results provide a framework to study regulatory modules directing expression of each polyamine metabolic gene.

DOI： 10.3390/cells10123283

researchmap

植物ポリアミンの代謝と機能招待査読

高橋卓, 本瀬宏康

化学と生物 59 ( 6 ) 290 - 297 2021年6月

Distinct functions of ethylene and ACC in the basal land plant marchantia polymorpha. 査読

Asuka Katayose, Asaka Kanda, Yasutaka Kubo, Taku Takahashi, Hiroyasu Motose

Plant & cell physiology 2021年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

Ethylene is a gaseous phytohormone involved in various physiological processes including fruit ripening, senescence, root hair development, and stress responses. Recent genomics have suggested that most homologous genes of ethylene biosynthesis and signaling are conserved from algae to angiosperms, whereas function and biosynthesis of ethylene remain unknown in basal plants. Here, we examined physiological effects of ethylene, an ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), and an inhibitor of ethylene perception, silver thiosulfate (STS), in a basal land plant Marchantia polymorpha. M. polymorpha plants biosynthesized ethylene and treatment with high concentrations of ACC slightly promoted ethylene production. ACC remarkably suppressed growth of thalli (vegetative organs) and rhizoids (root-hair-like cells), whereas exogenous ethylene slightly promoted thallus growth. STS suppressed thallus growth and induced ectopic rhizoid formation on the dorsal surface of thalli. Thus, ACC and ethylene have different effects on vegetative growth of M. polymorpha. We generated single and double mutants of ACC synthase-like (ACSL) genes, MpACSL1 and MpACSL2. They did not show obvious defects in thallus growth, ACC content, and ethylene production, indicating that MpACSL genes are not essential for the vegetative growth and the biosynthesis of ACC and ethylene. The gene expression analysis suggested involvement of MpACSL1 and MpACSL2 in stress responses. Collectively, our results imply ethylene-independent function of ACC and the absence of ACC-mediated ethylene biosynthesis in M. polymorpha.

DOI： 10.1093/pcp/pcab042

PubMed

researchmap

The root growth reduction in response to mechanical stress involves ethylene-mediated microtubule reorganization and transmembrane receptor-mediated signal transduction in Arabidopsis. 査読国際誌

Takashi Okamoto, Shogo Takatani, Hiroyasu Motose, Hidetoshi Iida, Taku Takahashi

Plant cell reports 40 ( 3 ) 575 - 582 2021年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

KEY MESSAGE: We found that mutations in a Ca2+-permeable mechanosensitive channel MCA1, an ethylene-regulated microtubule-associated protein WDL5, and a versatile co-receptor BAK1 affect root growth response to mechanical stress. Plant root tips exposed to mechanical impedance show a temporal reduction in the elongation growth. The process involves a transient Ca2+ increase in the cytoplasm followed by ethylene signaling. To dissect the molecular mechanisms underlying this response, we examined the root growth of a series of Arabidopsis mutants with potentially altered response to mechanical stress after transfer from vertical to horizontal plates that were covered by dialysis membrane as an impedance. Among the plant hormone-response mutants tested, the ethylene-insensitive mutant ein3 was confirmed to show no growth reduction after the transfer. The root growth reduction was attenuated in a mutant of MCA1 encoding a Ca2+-permeable mechanosensitive channel and that of WDL5 encoding an ethylene-regulated microtubule-associated protein. We also found that the growth reduction was enhanced in a mutant of BAK1 encoding a co-receptor that pairs with numerous leucine-rich repeat receptor kinases to modulate growth and immunity. These results suggest the root growth reduction in response to mechanical stress involves ethylene-mediated microtubule reorganization and also transmembrane receptor-mediated signal transduction.

DOI： 10.1007/s00299-020-02653-6

PubMed

researchmap

Ceramides mediate positional signals in Arabidopsis thaliana protoderm differentiation. 査読国際誌

Kenji Nagata, Toshiki Ishikawa, Maki Kawai-Yamada, Taku Takahashi, Mitsutomo Abe

Development (Cambridge, England) 148 ( 2 ) 2021年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

The differentiation of distinct cell types in appropriate patterns is a fundamental process in the development of multicellular organisms. In Arabidopsis thaliana, protoderm/epidermis differentiates as a single cell layer at the outermost position. However, little is known about the molecular nature of the positional signals that achieve correct epidermal cell differentiation. Here, we propose that very-long-chain fatty acid-containing ceramides (VLCFA-Cers) mediate positional signals by stimulating the function of ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1), a master regulator of protoderm/epidermis differentiation, during lateral root development. We show that VLCFA-Cers, which are synthesized predominantly in the outermost cells, bind to the lipid-binding domain of ATML1. Importantly, this cell type-specific protein-lipid association alters the activity of ATML1 protein and consequently restricts its expression to the protoderm/epidermis through a transcriptional feedback loop. Furthermore, establishment of a compartment, enriched with VLCFA-containing sphingolipids, at the outer lateral membrane facing the external environment may function as a determinant of protodermal cell fate. Taken together, our results indicate that VLCFA-Cers play a pivotal role in directing protoderm/epidermis differentiation by mediating positional signals to ATML1.This article has an associated 'The people behind the papers' interview.

DOI： 10.1242/dev.194969

PubMed

researchmap

Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana. 査読国際誌

Takahiro Tanaka, Yuichi Nishii, Hirotoshi Matsuo, Taku Takahashi

Plants (Basel, Switzerland) 9 ( 6 ) 2020年6月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on Arabidopsis mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and Ler-0 accessions that can be detected in standard agarose gel electrophoresis.

DOI： 10.3390/plants9060779

PubMed

researchmap

Chemical Synthesis and Biological Effect on Xylem Formation of Xylemin and Its Analogues 査読

Hiroyoshi Takamura, Hiroyasu Motose, Taichi Otsu, Shiori Shinohara, Ryugo Kouno, Isao Kadota, Taku Takahashi

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY 2020 ( 18 ) 2745 - 2753 2020年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY-V C H VERLAG GMBH

Xylemin (6) and its designed structural analogues 18-23, N-(4-aminobutyl)alkylamines, were synthesized by 2-nitrobenzenesulfonamide (Ns) strategy. Investigation of the improved synthesis of 20-23 resulted in the development of one-step synthesis of these analogues from the commercially available corresponding ketones. Biological assessment of the synthetic molecules elucidated that xylemin (6) and the analogue N-(4-aminobutyl)cyclopentylamine (21) promoted the expression level of thermospermine synthase ACAULIS5 (ACL5) and enhanced xylem formation. In addition, xylemin (6) was found to significantly promote lateral root formation, whereas xylemin analogues 18-23 including 21 did not. These results indicate that the analogue 21 has the potential as a novel inhibitor of thermospermine synthesis to work specifically in xylem differentiation.

DOI： 10.1002/ejoc.202000322

Web of Science

researchmap

Plant Polyamines. 国際誌

Taku Takahashi

Plants (Basel, Switzerland) 9 ( 4 ) 2020年4月

記述言語：英語

Polyamines are small organic compounds found in all living organisms. According to the high degree of positive charge at physiological pH, they interact with negatively charged macromolecules, such as DNA, RNA, and proteins, and modulate their activities. In plants, polyamines, some of which are presented as a conjugated form with cinnamic acids and proteins, are involved in a variety of physiological processes. In recent years, the study of plant polyamines, such as their biosynthetic and catabolic pathways and the roles they play in cellular processes, has flourished, becoming an exciting field of research. There is accumulating evidence that polyamine oxidation, the main catabolic pathway of polyamines, may have a potential role as a source of hydrogen peroxide. The papers in this Special Issue highlight new discoveries and research in the field of plant polyamine biology. The information will help to stimulate further research and make readers aware of the link between their own work and topics related to polyamines.

DOI： 10.3390/plants9040511

PubMed

researchmap

Microtubule Response to Tensile Stress Is Curbed by NEK6 to Buffer Growth Variation in the Arabidopsis Hypocotyl. 査読国際誌

Shogo Takatani, Stéphane Verger, Takashi Okamoto, Taku Takahashi, Olivier Hamant, Hiroyasu Motose

Current biology : CB 2020年3月

記述言語：英語

Growth variability generates mechanical conflicts in tissues. In plants, cortical microtubules usually align with maximal tensile stress direction, thereby mechanically reinforcing cell walls, and channeling growth rate and direction. How this is achieved remains largely unknown and likely involves microtubule regulators. The NIMA-related microtubule-associated kinase NEK6 phosphorylates tubulin, leading to the depolymerization of a subset of microtubules. We found that cortical microtubules exhibit a hyper-response to mechanical stress in the nek6 mutant. This response contributes to local cell protrusions in slow-growing regions of the nek6 mutant hypocotyl. When growth amplitude is higher, the hyper-alignment of microtubules leads to variable, stop-and-go, phenotypes, resulting in wavy hypocotyl shapes. After gravistimulation or touch, the nek6 mutant also exhibits a hyperbent hypocotyl phenotype, consistent with an enhanced perception of its own deformation. Strikingly, we find that NEK6 exhibits a novel form of polarity, being recruited at the ends of a subset of microtubules at cell edges. This pattern can be modified after local ablation, matching the new maximal tensile stress directions. We propose that NEK6 depolymerizes cortical microtubules that best align with maximal tensile stress to generate a noisier network of microtubules. This prevents an overreaction of microtubules to growth fluctuations and, instead, promotes the buffering of growth variations.

DOI： 10.1016/j.cub.2020.02.024

PubMed

researchmap

　前のページ - 次のページ　

<髙橋 卓>

論文 - 髙橋 卓

<髙橋　卓>

論文 - 髙橋　卓