研究者詳細 - 髙橋　卓

論文 - 髙橋　卓

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Mutations in ribosomal proteins, RPL4 and RACK1, suppress the phenotype of a thermospermine-deficient mutant of Arabidopsis thaliana（共著）査読

J. I. Kakehi, E. Kawano, K. Yoshimoto, Q. Cai, A. Imai, T. Takahashi

PLoS ONE 10 ( 1 ) e0117309 2015年1月

Thermospermine modulates expression of auxin-related genes in Arabidopsis（共著）査読

W. Tong, K. Yoshimoto, J.-I. Kakehi, H. Motose, M. Niitsu, T. Takahashi

Front. Plant Sci. 5 ( 94 ) 2014年3月

Allele-specific effects of PDF2 on floral morphology in Arabidopsis thaliana 査読

Naoko Kamata, Ayaka Sugihara, Yoshibumi Komeda, Taku Takahashi

Plant Signaling and Behavior 8 ( 12 ) 1 - 3 2014年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

The class IV Homeodomain-leucine zipper (HD-ZIP IV) gene family includes several genes that are functionally significant in epidermal development. Our recent study revealed that double mutants of the epidermis-expressed HD-ZIP IV members, PROTODERMAL FACTOR2 (PDF2) in combination with some HOMEODOMAIN GLABROUS (HDG, pronounced "hedge") genes, affect stamen development and specification of petal and stamen identity, possibly in a non cell-autonomous manner. However, the effect of the pdf2 mutations on the floral development was largely different depending on T-DNA insertion locations: pdf2-1 hdg flowers exhibited homeotic conversion of petals and stamens, while pdf2-2 hdg flowers had only a reduced number of stamens. Here, we used 2 additional pdf2 alleles to make double mutants and found that their floral phenotypes were rather similar to those of pdf2-2 hdg. The allele-specific effect caused by pdf2-1, which carries a T-DNA in a steroidogenic acute regulatory protein-related lipid transfer (START) domainencoding region, suggests the importance of the START domain in proper function of HD-ZIP IV proteins. © 2014 Landes Bioscience.

DOI： 10.4161/psb.27417

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Mutations in epidermis-specific HD-ZIP IV genes affect floral organ identity in Arabidopsis thaliana.（共著）査読

Kamata N, Okada H, Komeda Y, Takahashi T

The Plant Journal 75 ( 3 ) 430 - 440 2013年8月

Determination of polyamines in Arabidopsis thaliana by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent 査読

Genki Inoue, Takashi Kaneta, Toshio Takayanagi, Junichi Kakehi, Hiroyasu Motose, Taku Takahashi

ANALYTICAL METHODS 5 ( 11 ) 2854 - 2859 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ROYAL SOC CHEMISTRY

Herein, we report a novel method for the determination of polyamines in a sample extracted from Arabidopsis thaliana by capillary electrophoresis (CE) using salicylaldehyde-5-sulfonate (SAS) as a derivatizing reagent. An aldehyde group of SAS forms a Schiff base with amino groups of aliphatic polyamines, resulting in an anionic species with an absorption band in the ultraviolet region. The derivatization method was straightforward since the derivatives were formed by mixing a sample with the derivatizing reagent at a neutral pH. In addition, the negative charges induced by SAS led to a high resolution with a short analysis time. This method permitted the separation of five polyamines, which play important roles in plants. However, further improvement in sensitivity was needed for the determination of the polyamines in plant samples. Therefore, the CE method was coupled with solid-phase extraction (SPE) using an ion-pairing formation with sodium dodecyl benzene sulfonate. The SPE method improved the concentration limits of detection to sub-mu M levels, which corresponded with a 10-fold enhancement. The calibration curves for cadaverine, putrescine, and spermidine were linear with concentrations that ranged from 1 to 20 mu M and correlation coefficients (R-2) were greater than 0.998. The proposed method was applied to the determination of spermidine in a plant sample, Arabidopsis thaliana.

DOI： 10.1039/c3ay26360f

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NIMA-related kinases regulate directional cell growth and organ development through microtubule function in Arabidopsis thaliana.（共著）査読国際誌

Motose H, Takatani S, Ikeda T, Takahashi T

Plant Signaling Behavior 7 ( 12 ) 1552 - 5 2012年12月

Thermospermine suppresses auxin-inducible xylem differentiation in Arabidopsis thaliana. （共著）査読

Yoshimoto K, Noutoshi Y, Hayashi K, Shirasu K, Takahashi T, Motose H

Plant Signaling Behavior 7 ( 8 ) 937 - 939 2012年8月

Thermospermine is Not a Minor Polyamine in the Plant Kingdom 査読

Ayaka Takano, Jun-Ichi Kakehi, Taku Takahashi

PLANT AND CELL PHYSIOLOGY 53 ( 4 ) 606 - 616 2012年4月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OXFORD UNIV PRESS

Thermospermine is a structural isomer of spermine, which is one of the polyamines studied extensively in the past, and is produced from spermidine by the action of thermospermine synthase encoded by a gene named ACAULIS5 (ACL5) in plants. According to recent genome sequencing analyses, ACL5-like genes are widely distributed throughout the plant kingdom. In Arabidopsis, ACL5 is expressed specifically during xylem formation from procambial cells to differentiating xylem vessels. Loss-of-function mutants of ACL5 display overproliferation of xylem vessels along with severe dwarfism, suggesting that thermospermine plays a role in the repression of xylem differentiation. Studies of suppressor mutants of acl5 that recover the wild-type phenotype in the absence of thermospermine suggest that thermospermine acts on the translation of specific mRNAs containing upstream open reading frames (uORFs). Thermospermine is a novel type of plant growth regulator and may also serve in the control of wood biomass production.

DOI： 10.1093/pcp/pcs019

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A chemical biology approach reveals an opposite action between thermospermine and auxin inxylem development in Arabidopsis thaliana.（共著）査読

Yoshimoto K, Noutoshi Y, Hayashi K, Shirasu K, Takahashi T, Motose H

Plant and Cell Physiology 53 ( 4 ) 635 - 645 2012年4月

Key proliferative activity in the junction between the leaf blade and leaf petiole of Arabidopsis thaliana.（共著）査読

Ichihashi Y, Kawade K, Usami T, Horiguchi G, Takahashi T, Tsukaya H

Plant Physiology 157 ( 3 ) 1151 - 1162 2011年11月

NIMA-related kinases 6, 4, and 5 interact with each other to regulate microtubule organization during epidermal cell expansion in Arabidopsis thaliana.（共著）査読

Motose H, Hamada T, Yoshimoto K, Murata T, Hasebe M, Watanabe Y, Hashimoto T, Sakai T, Takahashi T

The Plant Journal 67 ( 6 ) 993 - 1005 2011年9月

Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development. （共著）査読

M. Shirakawa, H. Ueda, T. Shimada, Y. Koumoto, T.-L. Shimada, M. Kondo, T. Takahashi, Y. Okuyama, M. Nishimura, I. Hara-Nishimura

Plant J. 64 ( 6 ) 924 - 935 2010年12月

Norspermine substitutes for thermospermine in the control of stem elongation in Arabidopsis thaliana. （共著）査読

J.-I. Kakehi, Y. Kuwashiro, H. Motose, K. Igarashi, T. Takahashi

FEBS Lett. 584 ( 14 ) 3042 - 3046 2010年7月

Polyamines: Ubiquitous Polycations with Unique Roles in Growth and Stress Responses.（共著）査読

T. Takahashi, J.-I. Kakehi

Ann. Bot. 105 ( 1 ) 1 - 6 2010年1月

シロイヌナズナSYP2ファミリーSNAREタンパク質の機能解析

白川一, 上田晴子, 西山千晶, 嶋田知生, 河本恭子, 近藤真紀, 高橋卓, 西村幹夫, 西村いくこ

日本植物生理学会年会およびシンポジウム講演要旨集 2010 ( 0 ) 127 - 127 2010年

出版者・発行元：日本植物生理学会

シロイヌナズナゲノムには4種類のSYP2ファミリーSNAREタンパク質がコードされている．過去に，VAM3/SYP22 及びPEP12/SYP21の単独変異体は致死性を示すことが報告されている．しかしながら，我々はこれまで，vam3/syp22変異体を単離することに成功し，変異体の解析から，VAM3/SYP22が植物の成長・維管束パターンの形成・ミロシン細胞分化に関与していることを報告してきた．このことから，SYP2ファミリーの機能について再検証が必要であると考えられる．今回，我々はplp/syp23の変異体を確立することに成功し，加えて，vam3/syp22との多重変異体を作製した．さらに，artificial microRNAによる遺伝子抑制法により，vam3/syp22変異体背景でPEP12/SYP21の遺伝子発現を抑制することに成功した．これらの変異体の表現型の解析から，VAM3/SYP22，PEP12/SYP21，PLP/SYP23が冗長的に機能していることを発見した．興味深いことに，GFP融合タンパク質を用いた細胞内局在の解析により，PLP/SYP23が膜成分に局在することなくSNAREタンパク質として機能している可能性を見出した．そこで，本大会ではSYP2ファミリーの機能について議論したい．

DOI： 10.14841/jspp.2010.0.0127.0

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Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana 査読

Tomonori Deguchi, Mariko Itoh, Hiroko Urawa, Tomohiro Matsumoto, Sohei Nakayama, Takashi Kawasaki, Takeshi Kitano, Shoji Oda, Hiroshi Mitani, Taku Takahashi, Takeshi Todo, Junichi Sato, Kiyotaka Okada, Kohei Hatta, Shunsuke Yuba, Yasuhiro Kamei

Development Growth and Differentiation 51 ( 9 ) 769 - 775 2009年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish
Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants. © 2009 Japanese Society of Developmental Biologists.

DOI： 10.1111/j.1440-169X.2009.01135.x

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A semi-dominant mutation in a ribosomal protein L10 gene suppresses the dwarf phenotype of the acl5 mutant in Arabidopsis thaliana. （共著）査読

A. Imai, M. Komura, E. Kawano, Y. Kuwashiro, T. Takahashi

The Plant Journal 56 ( 6 ) 881 - 890 2008年12月

Thermospermine is required for stem elongation in Arabidopsis thaliana. （共著）査読

J.-I. Kakehi, Y. Kuwashiro, M. Niitsu, T. Takahashi

Plant and Cell Physiology 49 ( 9 ) 1342 - 1349 2008年9月

ミロシン細胞分化におけるSYP2ファミリーSNAREタンパク質の役割

上田晴子, 白川一, 西山千晶, 嶋田知生, 高橋卓, 西村いくこ

日本植物生理学会年会およびシンポジウム講演要旨集 2008 ( 0 ) 794 - 794 2008年

出版者・発行元：日本植物生理学会

ミロシン細胞はアブラナ科に特異的な異型細胞である．葉のミロシン細胞は主に維管束近傍に点在し，周囲の細胞に比べて顕著に伸張し分枝する場合もある．ミロシン細胞の液胞は発達しており，内部に蓄積されたミロシナーゼがグルコシノレート（カラシ油配糖体）を分解して辛み成分を生成することによって植物の生体防御に関与する．現在のところ，ミロシン細胞の分化機構は全く未知である．これまで，ミロシン細胞の数が増加して異常な分布パターンを示すatvam3変異体の解析から，ミロシン細胞と導管の分化が植物ホルモンによって制御されている可能性を報告してきた．AtVAM3は液胞膜と液胞前区画膜に局在するSYP2ファミリーに属するSNAREタンパク質である．シロイヌナズナにはAtVAM3以外に2つのSYP2タンパク質 (AtPEP12およびAtPLP) が存在することから，ミロシン細胞分化におけるこれらの関与を解析した．その結果，atpep12変異体およびatplp変異体では，ミロシン細胞の数と分布パターンに異常はなかった．atvam3変異体との多重変異体では，atvam3単独変異体よりもミロシン細胞分化において顕著な異常を示し，さらにatvam3単独変異体では見られなかった新たな表現型を持つことが明らかとなった．以上の結果から，SYP2ファミリーの機能について考察したい．

DOI： 10.14841/jspp.2008.0.0794.0

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A protective role for the polyamine spermine against drought stress in Arabidopsis 査読

Koji Yamaguchi, Yoshihiro Takahashi, Thomas Berberich, Akihiko Imai, Taku Takahashi, Anthonv J. Michael, Tomonobu Kusano

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 352 ( 2 ) 486 - 490 2007年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE

Cellular polyamine content often changes in response to abiotic stresses. However, its physiological relevance is unknown. We found that an Arabidopsis mutant plant (acl5lspms), which cannot produce spermine, is hypersensitive to high salt. Examination of drought sensitivity of the mutant and comparison with wild type plants indicated hypersensitivity to drought. This phenotype was cured by spermine pretreatment but not by the other polyamines putrescine and spermidine, suggesting that drought-hypersensitivity exhibited by the mutant is due to spermine deficiency. The water loss rate of wild type and mutant plants were similar until 20 min after onset of dehydration stress, but after a longer exposure the rate in mutant plants was higher than in wild type plants. Consistent with this result, the stomata of the mutant leaves remained open while in wild type leaves they closed. Based on the collected data, we discuss a role for spermine in response to drought stress. (c) 2006 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2006.11.041

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