Faculty Profiles - IIDA Seiji

Other Link： https://jlc.jst.go.jp/DN/JLC/20009068467?from=CiNii

岡山大学病院口腔がん専門外来と岡山市歯科医師会の連携による口腔がん検診研修会の概要とアンケート結果について

岸本晃治, 岡崎景, 西山明慶, 居樹秀明, 松村達志, 伊原木聰一郎, 銅前昇平, 吉岡徳枝, 栗尾奈愛, 矢尾真弓, 小畑協一, 尾野裕子, 志茂剛, 壺内智郎, 飯田征二, 佐々木朗

岡山歯学会雑誌 33 ( 2 ) 23 - 28 2014.12

Language：Japanese Publisher：岡山歯学会

口腔癌検診研修会基礎コースを修了した歯科医師を対象に、研修会終了後にアンケート調査を行った。2013年に行われた3回の研修会の研修者の合計は46人(3人が2回研修)であった。臨床経験年数は10〜42年(平均23.6年、中央値25年)に比較的長い研修者が多かった。口腔癌症例経験ありは70%に認められ、経験症例数は1〜3例が多かった。口腔癌症例経験者の中で癌の疑いがあると診断して専門機関に紹介した症例が93%と大部分を占めた。しかし、その中で早期癌を診断できた症例は45%に過ぎず、初診時に進行していた症例も46例に認められた。また、紹介が少し遅くなった症例が9%認められたが患者が専門機関をなかなか受診してくれなかった例も1例あった。癌の部位は舌が最も多く41%認められ、下顎歯肉32%、口底13%と続いた。研修会の時間・レベルは概ね適切で、講義・実習・テキストもためになり、今後の臨床に役立つとの回答が得られた。再研修の必要性は91%と多かったが、アドバンスコースの希望者は69%に留まり、ベーシックコースの希望者も22%認められた。また、再研修までの期間は1年の35%を前後にばらつきが認められた。研修者はより実践的に役立つ研修内容を希望していた。

Assessment of intraoral mucosal pain induced by the application of capsaicin

Ryuji Terayama, Kotaro Maruhama, Hiroki Tsuchiya, Masahide Mizutani, Seiji Iida, Tomosada Sugimoto

Archives of Oral Biology 59 ( 12 ) 1334 - 1341 2014.12

Surrounding cells affect the gene expression pattern of human beta-defensins in squamous cell carcinoma in vitro

Anticancer Res 34 ( 11 ) 6443 - 6449 2014.11

Effect of geometry and microstructure of honeycomb TCP scaffolds on bone regeneration Reviewed

Kiyofumi Takabatake, Eiki Yamachika, Hidetsugu Tsujigiwa, Yasushi Takeda, Mariko Kimura, Shin Takagi, Hitoshi Nagatsuka, Seiji Iida

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 102 ( 9 ) 2952 - 2960 2014.9

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

In recent years, artificial biological materials have been commonly used for the treatment of bone tissue defects caused by trauma, tumors, or surgical stress. Although tricalcium phosphate (TCP) is a promising absorbent bone tissue reconstruction biomaterial, it has been reported that its biocompatibility and osteoconductivity depend on its preparation method and sintering temperature. In addition, although it is thought that the microenvironment produced by the extracellular matrix plays an important role in cell growth and differentiation, there have been few studies on how the geometric structure of artificial biological materials affects cells. In the present study, a new honeycomb TCP scaffold containing through-holes with diameters of 300 mm has been developed. The influence of the sintering temperature on the crystal structure and material properties of the honeycomb TCP scaffold was investigated using scanning electron microscopy and X-ray diffraction. Its biocompatibility and osteoconductivity were also evaluated by implantation into experimental animals. It was found that a beta-TCP scaffold sintered at 1200 degrees C exhibited high biocompatibility and osteoconductivity, and when it was loaded with BMP-2, it exhibited both osteoconductivity and osteoinductivity, promoting rapid bone formation in both ectopic and orthotopic areas. It is thus a highly promising bone reconstruction material that is expected to find clinical applications. (C) 2013 Wiley Periodicals, Inc.

DOI： 10.1002/jbm.a.34966

CCN2 as a Novel Molecule Supporting Energy Metabolism of Chondrocytes

Aya Maeda-Uematsu, Satoshi Kubota, Harumi Kawaki, Kazumi Kawata, Yoshiaki Miyake, Takako Hattori, Takashi Nishida, Norifumi Moritani, Karen M. Lyons, Seiji Iida, Masaharu Takigawa

Journal of Cellular Biochemistry 115 ( 5 ) 854 - 865 2014.5

Hemoglobin Receptor Protein from Porphyromonas gingivalis Induces Interleukin-8 Production in Human Gingival Epithelial Cells through Stimulation of the Mitogen-Activated Protein Kinase and NF-κB Signal Transduction Pathways

Yuki Fujita, Masaaki Nakayama, Mariko Naito, Eiki Yamachika, Tetsuyoshi Inoue, Koji Nakayama, Seiji Iida, Naoya Ohara

Infection and Immunity 82 ( 1 ) 202 - 211 2014.1

Publishing type：Research paper (scientific journal) Publisher：American Society for Microbiology

ABSTRACT

Periodontitis is an inflammatory disease of polymicrobial origin affecting the tissues supporting the tooth. The oral anaerobic bacterium Porphyromonas gingivalis , which is implicated as an important pathogen for chronic periodontitis, triggers a series of host inflammatory responses that promote the destruction of periodontal tissues. Among the virulence factors of P. gingivalis , hemoglobin receptor protein (HbR) is a major protein found in culture supernatants. In this study, we investigated the roles of HbR in the production of inflammatory mediators. We found that HbR induced interleukin-8 (IL-8) production in the human gingival epithelial cell line Ca9-22. p38 mitogen-activated protein kinase (MAPK) and extracellular signal-related kinase 1/2 (Erk1/2) were activated in HbR-stimulated Ca9-22 cells. Inhibitors of p38 MAPK (SB203580) and Erk1/2 (PD98059) blocked HbR-induced IL-8 production. Additionally, HbR stimulated the translocation of NF-κB-p65 to the nucleus, consistent with enhancement of IL-8 expression by activation of the NF-κB pathway. In addition, small interfering RNA (siRNA) targeting activating transcription factor 2 (ATF-2) or cyclic AMP-response element-binding protein (CREB) inhibited HbR-induced IL-8 production. Moreover, pretreatment with SB203580 and PD98059 reduced HbR-induced phosphorylation of CREB and ATF-2, respectively. Combined pretreatment with an inhibitor of NF-κB (BAY11-7082) and SB203580 was more efficient in inhibiting the ability of HbR to induce IL-8 production than pretreatment with either BAY11-7082 or SB203580 alone. Thus, in Ca9-22 cells, the direct activation of p38 MAPK and Erk1/2 by HbR caused the activation of the transcription factors ATF-2, CREB, and NF-κB, thus resulting in the induction of IL-8 production.

DOI： 10.1128/iai.01140-12

Voice characteristics before versus after mandibular setback surgery in patients with mandibular prognathism using nonlinear dynamics and conventional acoustic analyses Reviewed

Katsuaki Mishima, Norifumi Moritani, Hiroyuki Nakano, Asuka Matsushita, Seiji Iida, Yoshiya Ueyama

JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY 41 ( 8 ) 706 - 709 2013.12

Language：English Publishing type：Research paper (scientific journal) Publisher：CHURCHILL LIVINGSTONE

Objectives: The purpose of this study was to explore the voice characteristics of patients with mandibular prognathism, and to investigate the effects of mandibular setback surgery on these characteristics using nonlinear dynamics and conventional acoustic analyses.
Materials and methods: Sixteen patients (8 males and 8 females) who had skeletal 3, class III malocclusion without cleft palate, and who underwent a bilateral sagittal split ramus osteotomy (BSSRO), were enrolled. As controls, 50 healthy adults (25 males and 25 females) were enrolled. The mean first LEs (mLE1) computed for each one-second interval, and the fundamental frequency (F0) and frequencies of the first and second formant (F1, F2) were calculated for each Japanese vowel.
Results and conclusions: The mLE1s for /u/ in males, and /o/ in females and the F2s for /i/ and /u/ in males, changed significantly after BSSRO. Class III voice characteristics were observed in the mLE1s for /i/ in both males and females, in the F0 for /a/, /i/, /u/ and /o/ in females, and in the F1 and F2 for /a/ in males, and the F1 for /u/ and the F2 for /i/ in females. Most of these characteristics were preserved after BSSRO. (C) 2013 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jcms.2012.01.021

軟骨細胞の代謝の基本を支えるCCN2の重要性(Essential role of CCN2 that supports the basal energy metabolism in chondrocytes)

前田彩, 久保田聡, 三宅由晃, 河田かずみ, 服部高子, 西田崇, 森谷徳文, 川木晴美, カレン・ライアン, 飯田征二, 滝川正春

日本生化学会大会プログラム・講演要旨集 86回 2T06a - 15 2013.9

CCNファミリー研究のメルティングポット CCN2は軟骨細胞のエネルギー代謝に重要である

前田彩, 久保田聡, 川木晴美, 河田かずみ, 三宅由晃, 服部高子, 西田崇, 森谷徳文, 飯田征二, 滝川正春

Journal of Oral Biosciences Supplement 2013 95 - 95 2013.9

The Role of Bone Marrow-Derived Cells During the Bone Healing Process in the GFP Mouse Bone Marrow Transplantation Model Reviewed International journal

Hidetsugu Tsujigiwa, Yasuhisa Hirata, Naoki Katase, Rosario Rive, ra Buery, Ryo Tamamura, Satoshi Ito, Shin Takagi, Seiji Iida, Hitoshi Nagatsuka

Calcified Tissue International 92 ( 3 ) 296 - 306 2013.3

Language：English Publishing type：Research paper (scientific journal)

Bone healing is a complex and multistep process in which the origin of the cells participating in bone repair is still unknown. The involvement of bone marrow-derived cells in tissue repair has been the subject of recent studies. In the present study, bone marrow-derived cells in bone healing were traced using the GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) were transplanted into C57BL/6 J wild mice. After transplantation, bone injury was created using a 1.0-mm drill. Bone healing was histologically assessed at 3, 7, 14, and 28 postoperative days. Immunohistochemistry for GFP; double-fluorescent immunohistochemistry for GFP-F4/80, GFP-CD34, and GFP-osteocalcin; and double-staining for GFP and tartrate-resistant acid phosphatase were performed. Bone marrow transplantation successfully replaced the hematopoietic cells into GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts or osteocytes in the repair stage were GFP-negative, whereas osteoclasts in the repair and remodeling stages and hematopoietic cells were GFP-positive. The results indicated that bone marrow-derived cells might not differentiate into osteoblasts. The role of bone marrow-derived cells might be limited to adjustment of the microenvironment by differentiating into inflammatory cells, osteoclasts, or endothelial cells in immature blood vessels.

DOI： 10.1007/s00223-012-9685-3

Unilateral delayed eruption of a mandibular permanent canine and the maxillary first and second molars, and agenesis of the maxillary third molar Reviewed

Jae Hyun Park, Kiyoshi Tai, Seiji Iida

AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS 143 ( 1 ) 134 - 139 2013.1

Language：English Publishing type：Research paper (scientific journal) Publisher：MOSBY-ELSEVIER

Delayed tooth eruption can affect the accuracy of orthodontic diagnosis and treatment planning and could delay overall treatment. A girl, aged 7 years 4 months, who had unilateral delayed eruption of several teeth was successfully treated. The evaluation of delayed tooth eruption should be considered by the orthodontist because the role of these teeth can affect the overall treatment of malocclusion. In patients with delayed tooth eruption, careful and accurate diagnosis and treatment planning will allow the orthodontist to start treatment at the proper stage and might reduce the overall orthodontic treatment time. (Am J Orthod Dentofacial Orthop 2013;143:134-9)

DOI： 10.1016/j.ajodo.2011.11.024

Toluene Exposure Leads to a Change in Expression Patterns of β Defensins in the Mouse Tracheal Epithelium

Takeda Seiko, Yamaai Tomoichiro, Kaneda Yoshihiro, Mizukawa Nobuyoshi, Iida Seiji, Fujimaki Hidekazu

J Toxicol Pathol 26 ( 1 ) 35 - 40 2013

Language：English Publisher：JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY

Defensins are generally implicated in the quick resistance of epithelial surfaces to microbials; however, recent reports have indicated that defensins also have unknown purposes in relation to noninfectious diseases. In this study, the localization patterns of anti-microbial peptides, β defensins (BDs), in the tracheal epithelium of male C3H mice under exposure to toluene were analyzed by immunohistochemistry. Mice were exposed one to ten times to toluene for 30 min by nose-only inhalation. Expression of BDs was revealed by immunohistochemistry in serial sections of trachea after the final exposure. Expression of BD-1 was usually observed at almost the same levels in all exposure groups, and expression of BD-2 was observed in the control group; however, the signals for BD-2 decreased gradually with frequency of exposure. In the group exposed ten times, expression of BD-2 decreased to far lower than that of the control group. No expression of BD-3 was detected in any groups. Interestingly, expression of BD-4 increased to the maximum in the group exposed four times and decreased to a level lower than that of the control in the group exposed ten times. The results of the present study indicated that toluene gas might change the expression pattern of BDs in the tracheal epithelial cells. The oscillation of expression of BD-4 was quite characteristic and might contribute to morphological damage in on the epithelial cells.

DOI： 10.1293/tox.26.35

CiNii Article

CiNii Books

Maxillary Anterior Segmental Distraction Osteogenesis (MASDO) for Cleft Patients

Other Link： https://jlc.jst.go.jp/DN/JALC/10017550764?from=CiNii

KOGO Mikihiko, AIKAWA Tomonao, HARADA Takeshi, ISHIHAMA Kohji, HIGUCHI Masataka, USHIMURA Ayako, OKURA Masaya, IIDA Seiji

Bulletin Of Japanese Society for Jaw Deformities 22 S7 - S14 2012.12

bFGFによるマウス骨由来間葉系幹細胞の増殖

高畠清文, 山近英樹, 喜多憲一郎, 金田祥弘, 妹尾昌紀, 片岡陽平, 飯田征二

日本再生歯科医学会誌 10 ( 1 ) 52 - 52 2012.12

軟骨細胞の代謝システムにおけるCCN2の役割

前田彩, 久保田聡, 三宅由晃, 河田かずみ, 服部高子, 西田崇, 森谷徳文, 川木晴美, Lyons Karen M., 飯田征二, 滝川正春

岡山歯学会雑誌 31 ( 2 ) 93 - 94 2012.12

Solid-type primary intraosseous squamous cell carcinoma of the mandible: A case report with histopathological and imaging features Reviewed

Hidenobu Matsuzaki, Naoki Katase, Tatsushi Matsumura, Marina Hara, Yoshinobu Yanagi, Hitoshi Nagatsuka, Seiji Iida, Jun-Ichi Asaumi

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 114 ( 5 ) e71 - e77 2012.11

Language：English Publishing type：Research paper (scientific journal)

Primary intraosseous squamous cell carcinoma (PIOSCC) is a rare malignant odontogenic tumor arising from odontogenic epithelial remnants within the jawbones. PIOSCC is histopathologically divided into 3 types: solid-type carcinoma, carcinoma derived from a keratocystic odontogenic tumor, and carcinoma derived from an odontogenic cyst. In this article, we report a case of solid-type PIOSCC involving reactive bone formation in the mandible in a 60-year-old female patient together with its histopathological and imaging findings. © 2012 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.oooo.2012.05.019

Scopus

CCN2/CTGF欠損が軟骨細胞のエネルギー代謝に及ぼす影響

前田彩, 久保田聡, 服部高子, 西田崇, 飯田征二, 滝川正春

Journal of Oral Biosciences Supplement 2012 96 - 96 2012.9

The morphological features and developmental changes of the philtral dimple: a guide to surgical intervention in cases of cleft lip. Reviewed

Kishi N, Tanaka S, Iida S, Kogo M

Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery 40 ( 3 ) 215 - 222 2012.4

Language：English Publishing type：Research paper (scientific journal) Publisher：CHURCHILL LIVINGSTONE

Objective: This study was undertaken to analyse in detail the morphological features of the philtral dimple and the developmental changes of philtral morphology, including a comparison with patients with a cleft lip.Patients and participants: Fifty-five normal Japanese adults, seventy-five children and fifteen patients with complete unilateral cleft lip were referred for analysis.Design: 3D facial data were acquired with a non-contacting laser scanner. We calculated the desired linear and angular components, defining the features of the philtrum with analysing software.Results: The philtral dimple was morphologically expressed by the two different deepest points in the horizontal and sagittal sectional view, respectively. These decreased with age and did not show any correlation. In contrast, philtral measurements on the X-Y coordinates increased during the course of development. Most of the linear and angular variables in the patients with the cleft lip had exhibited greater values than the normal subjects, reflecting the deformed philtral morphology.Conclusions: To obtain an optimally natural philtral construction accompanied by a symmetrical lip in cleft surgery, it is critical to take into consideration both the geometric features of the philtral depth and age-appropriate morphological features of the philtrum as objective criteria. (C) 2011 European Association for Cranio-Maxillo-Facial Surgery.

DOI： 10.1016/j.jcms.2011.04.019

Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesenchymal stem cells (MSCs) and regeneration of bone from MSC in vivo Reviewed

Yamachika Eiki, Tsujigiwa Hidetsugu, Matsubara Masakazu, Hirata Yasuhisa, Kita Kenichiro, Takabatake Kiyofumi, Mizukawa Nobuyoshi, Kaneda Yoshihiro, Nagatsuka Hitoshi, Iida Seiji

JOURNAL OF MOLECULAR HISTOLOGY 43 ( 2 ) 223 - 233 2012.4

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Some progress has been made in development of methods to regenerate bone from cultured cells, however no method is put to practical use. Here, we developed methods to isolate, purify, and expand mesenchymal stem cells (MSCs) from mouse compact bone that may be used to regenerate bone in vivo. These cells were maintained in long-term culture and were capable of differentiating along multiple lineages, including chondrocyte, osteocyte, and adipocyte trajectories. We used standard cell isolation and culture methods to establish cell cultures from mouse compact bone and bone marrow. Cultures were grown in four distinct media to determine the optimal composition of culture medium for bone-derived MSCs. Putative MSCs were subjected to flow cytometry, alkaline phosphatase assays, immunohistochemical staining, and several differentiation assays to assess cell identity, protein expression, and developmental potential. Finally, we used an in vivo bone formation assay to determine whether putative MSCs were capable of regenerating bone. We found that compact bone of mice was a better source of MCSs than the bone marrow, that growth in plastic flasks served to purify MSCs from hematopoietic cells, and that MSCs grown in basic fibroblast growth factor (bFGF)-conditioned medium were, based on multiple criteria, superior to those grown in leukemia inhibitory factor-conditioned medium. Moreover, we found that the MSCs isolated from compact bone and grown in bFGF-conditioned medium were capable of supporting bone formation in vivo. The methods and results described here have implications for understanding MSC biology and for clinical purpose.

DOI： 10.1007/s10735-011-9385-8