研究者詳細 - HARA EMILIO SATOSHI

論文 - HARA EMILIO SATOSHI

分割表示 97 件中 21 - 40 件目／全件表示 >>

Self-adhering implantable device of titanium: Enhanced soft-tissue adhesion by sandblast pretreatment 査読

Atsushi Yabe, Masahiro Okada, Emilio Satoshi Hara, Yasuhiro Torii, Takuya Matsumoto

Colloids and Surfaces B: Biointerfaces 211 112283 - 112283 2022年3月

Suppression of Bone Necrosis around Tooth Extraction Socket in a MRONJ-like Mouse Model by E-rhBMP-2 Containing Artificial Bone Graft Administration 査読国際誌

Yukie Tanaka, Kyaw Thu Aung, Mitsuaki Ono, Akihiro Mikai, Anh Tuan Dang, Emilio Satoshi Hara, Ikue Tosa, Kei Ishibashi, Aya Ono-Kimura, Kumiko Nawachi, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 22 ( 23 ) 12823 - 12823 2021年11月

Immediate soft-tissue adhesion and the mechanical properties of the Ti–6Al–4V alloy after long-term acid treatment 査読国際誌

Yaming Wang, Masahiro Okada, Shi Chao Xie, Yu Yang Jiao, Emilio Satoshi Hara, Hiroaki Yanagimoto, Takumi Fukumoto, Takuya Matsumoto

Journal of Materials Chemistry B 9 ( 39 ) 8348 - 8354 2021年8月

Nanostructural analysis of distinct nucleation sites in pathological mineralization 査読

Emilio Satoshi Hara, Susumu Oozawa, Noriyuki Nagaoka, Masahiro Okada, Shingo Kasahara, Takuya Matsumoto

Materials Advances 2 ( 13 ) 4423 - 4431 2021年6月

Preclinical bioequivalence study of E.coli-derived rhBMP-2/β-TCP and autogenous bone in a canine guided-bone regeneration model. 査読

Shuji Nosho, Mitsuaki Ono, Taishi Komori, Akihiro Mikai, Ikue Tosa, Kei Ishibashi, Yukie Tanaka, Aya Kimura-Ono, Emilio S Hara, Toshitaka Oohashi, Takuo Kuboki

Journal of Prosthodontic Research 66 ( 1 ) 124 - 130 2021年6月

Tryptophan and Kynurenine Enhances the Stemness and Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro and In Vivo 査読

Hai Pham, Mitsuaki Ono, Emilio S. Hara, Ha Nguyen, Anh Dang, Hang Do, Taishi Komori, Ikue Tosa, Yuri Hazehara-Kunitomo, Yuya Yoshioka, Yasutaka Oida, Kentaro Akiyama, Takuo Kuboki

Materials 14 ( 1 ) 208 - 208 2021年1月

Micro-Architectural Investigation of Teleost Fish Rib Inducing Pliant Mechanical Property 査読国際誌

Yu Yang Jiao, Masahiro Okada, Emilio Satoshi Hara, Shi Chao Xie, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Materials 13 ( 22 ) 5099 - 5099 2020年11月

Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model 査読

Kyaw Thu Aung, Kentaro Akiyama, Masayoshi Kunitomo, Aung Ye Mun, Ikue Tosa, Ha Thi Thu Nguyen, Jiewen Zhang, Teisaku Kohno, Mitsuaki Ono, Emilio Satoshi Hara, Takuo Kuboki

International Journal of Molecular Sciences 21 ( 21 ) 8103 - 8103 2020年10月

Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments 査読

Shuji Nosho, Ikue Tosa, Mitsuaki Ono, Emilio Satoshi Hara, Kei Ishibashi, Akihiro Mikai, Yukie Tanaka, Aya Kimura-Ono, Taishi Komori, Kenji Maekawa, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 21 ( 21 ) 7967 - 7967 2020年10月

BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model 査読

Akihiro Mikai, Mitsuaki Ono, Ikue Tosa, Ha Thi Thu Nguyen, Emilio Satoshi Hara, Shuji Nosho, Aya Kimura-Ono, Kumiko Nawachi, Takeshi Takarada, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 21 ( 19 ) 7028 - 7028 2020年9月

Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis 査読

Mst Nahid Akhter, Emilio Hara, Koichi Kadoya, Masahiro Okada, Takuya Matsumoto

International Journal of Molecular Sciences 21 ( 15 ) 5327 - 5327 2020年7月

掲載種別：研究論文（学術雑誌）出版者・発行元：{MDPI} {AG}

Current stem cell-based techniques for bone-like tissue synthesis require at least two to three weeks. Therefore, novel techniques to promote rapid 3D bone-like tissue synthesis in vitro are still required. In this study, we explored the concept of using cell nanofragments as a substrate material to promote rapid bone formation in vitro. The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. The results showed that ultrasonication for 3 min allowed the fabrication of homogeneous nanofragments of less than 150 nm in length, which mineralized surprisingly in just one day, faster than the fragments obtained from all other methods. Further optimization of culture conditions indicated that a concentration of 10 mM or 100 mM of β-glycerophosphate enhanced, whereas fetal bovine serum (FBS) inhibited in a concentration-dependent manner, the mineralization of the cell nanofragments. Finally, a 3D collagen-cell nanofragment-mineral complex mimicking a bone-like structure was generated in just two days by combining the cell nanofragments in collagen gel. In conclusion, sonication for three min could be applied as a novel method to fabricate cell nanofragments of less than 150 nm in length, which can be used as a material for in vitro bone tissue engineering.

DOI： 10.3390/ijms21155327

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Titanium as an Instant Adhesive for Biological Soft Tissue 査読

Masahiro Okada, Emilio Satoshi Hara, Atsushi Yabe, Kei Okada, Yo Shibata, Yasuhiro Torii, Takayoshi Nakano, Takuya Matsumoto

Advanced Materials Interfaces 7 ( 9 ) 1902089 - 1902089 2020年5月

Association of masticatory muscle activity with sleep arousal and other concomitant movements during sleep 査読

Haruna Miki, Hajime Minakuchi, Mayu Miyagi, Emilio Satoshi Hara, Shuji Shigemoto, Yoshitaka Suzuki, Kenji Maekawa, Yoshizo Matsuka, Glenn T. Clark, Takuo Kuboki

Journal of Oral Rehabilitation 2020年3月

Vibratory splint therapy for decreasing sleep clenching: A pilot study 査読

Emilio Satoshi Hara, Andrea Lusvarghi Witzel, Hajime Minakuchi, Carlos Eduardo de Pitta, Rosane Tronquin Gallo, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Marcelo Costa Bolzan

CRANIO: The Journal of Craniomandibular & Sleep Practice 38 ( 1 ) 15 - 21 2020年1月

DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation 査読

Yu Nomura, Emilio Satoshi Hara, Yuya Yoshioka, Há Thi Nguyen, Shuji Nosho, Taishi Komori, Kei Ishibashi, Toshitaka Oohashi, Mitsuaki Ono, Takuo Kuboki

Cells Tissues Organs 207 ( 3-4 ) 115 - 126 2019年10月

担当区分：責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：S. Karger AG

Stem cells have essential applications in in vitro tissue engineering or regenerative medicine. However, there is still a need to understand more deeply the mechanisms of stem cell differentiation and to optimize the methods to control stem cell function. In this study, we first investigated the activity of DNA methyltransferases (DNMTs) during chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) and found that DNMT3A and DNMT3B were markedly upregulated during hBMSC chondrogenic differentiation. In an attempt to understand the effect of DNMT3A and DNMT3B on the chondrogenic differentiation of hBMSCs, we transiently transfected the cells with expression vectors for the two enzymes. Interestingly, DNMT3A overexpression strongly enhanced the chondrogenesis of hBMSCs, by increasing the gene expression of the mature chondrocyte marker, collagen type II, more than 200-fold. Analysis of the methylation condition in the cells revealed that DNMT3A and DNMT3B methylated the promoter sequence of early stem cell markers, NANOG and POU5F1(OCT-4). Conversely, the suppression of chondrogenic differentiation and the increase in stem cell markers of hBMSCs were obtained by chemical stimulation with the demethylating agent, 5-azacitidine. Loss-of-function assays with siRNAs targeting DNMT3A also significantly suppressed the chondrogenic differentiation of hBMSCs. Together, these results not only show the critical roles of DNMTs in regulating the chondrogenic differentiation of hBMSCs, but also suggest that manipulation of DNMT activity can be important tools to enhance the differentiation of hBMSCs towards chondrogenesis for potential application in cartilage tissue engineering or cartilage regeneration.

DOI： 10.1159/000502885

PubMed

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Type XVIII Collagen Modulates Keratohyalin Granule Formation and Keratinization in Oral Mucosa 査読

Ha Thi Thu Nguyen, Mitsuaki Ono, Emilio Hara, Taishi, Midori Edamatsu, Tomoko Yonezawa, Aya Kimura-Ono, Kenji Maekawa, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 20 ( 19 ) 4739 - 4739 2019年9月

Fibronectin-induced ductal formation in salivary gland self-organization model 査読

Mahmoud Farahat, Gulsan A. S. Kazi, Hiroaki Taketa, Emilio S. Hara, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

DEVELOPMENTAL DYNAMICS 248 ( 9 ) 813 - 825 2019年9月

Improvement of mechanical properties of Y-TZP by thermal annealing with monoclinic zirconia nanoparticle coating 査読国際誌

Masahiro Okada, Hiroaki Taketa, Emilio Satoshi Hara, Yasuhiro Torii, Masao Irie, Takuya Matsumoto

Dental Materials 35 ( 7 ) 970 - 978 2019年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier BV

OBJECTIVE: To assess whether a thermal annealing with a monoclinic zirconia (mZrO2) nanoparticle coating can improve the reliability of sandblasted yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) and maintain its mechanical strength. METHODS: Commercially available Y-TZP (Lava Frame, 3M Dental Products) disks were sintered and surface-treated as follows: AS (as sintered, with no treatment); SB (sandblasting); SB-TA (sandblasting followed by thermal annealing at 1000 °C); and SB-mZr-TA (sandblasting followed by thermal annealing at 1000 °C with the mZrO2 nanoparticle coating). The mZrO2 nanoparticles of 21 nm in size were prepared by a hydrothermal method, and coated onto Y-TZP sintered disks as a 5 g/L ethanol dispersion. Biaxial flexural strength (S) was measured using the piston-on-three-ball test, and reliability was evaluated by the Weibull modulus (m). RESULTS: Biaxial flexural tests showed a significant increase in the strength of Group SB (SSB = 1445 ± 191 MPa) compared with Group AS (SAS = 1071 ± 112 MPa). The thermal annealing improved the reliabilities of the sandblasted Y-TZP (mSB-TA = 20.14 and mSB-mZr-TA = 21.33), as compared with Group SB (mSB = 7.77). However, the conventional thermal annealing without the mZrO2 coating caused a significant decrease in the strength of sandblasted Y-TZP (SSB-TA = 1273 ± 65 MPa). Importantly, the mZrO2 coating prevented the decrease in the strength caused by conventional thermal annealing (SSB-mZr-TA = 1379 ± 65 MPa). SIGNIFICANCE: The thermal annealing with the mZrO2 nanoparticle coating can improve the reliability of sandblasted Y-TZP and maintain its mechanical strength, which would otherwise be decreased by the conventional annealing process.

DOI： 10.1016/j.dental.2019.04.002

PubMed

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Biomimetic mineralization using matrix vesicle nanofragments 査読

Yosuke Kunitomi, Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takuo Kuboki, Takayoshi Nakano, Hiroshi Kamioka, Takuya Matsumoto

Journal of Biomedical Materials Research Part A 107 ( 5 ) 1021 - 1030 2019年5月

Intermediate Water on Calcium Phosphate Minerals: Its Origin and Role in Crystal Growth 査読

Masahiro Okada, Emilio Satoshi Hara, Daisuke Kobayashi, Shoki Kai, Keiko Ogura, Masaru Tanaka, Takuya Matsumoto

ACS Applied Bio Materials 2019年3月

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