研究者詳細 - HARA EMILIO SATOSHI

論文 - HARA EMILIO SATOSHI

分割表示 >> ／全件表示 97 件中 1 - 97 件目

The cell membrane as biofunctional material for accelerated bone repair. 査読国際誌

Emi Hatano, Nahid Akhter, Risa Anada, Mitsuaki Ono, Toshitaka Oohashi, Takuo Kuboki, Hiroshi Kamioka, Masahiro Okada, Takuya Matsumoto, Emilio Satoshi Hara

Acta biomaterialia 2024年7月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

The cell (plasma) membrane is enriched with numerous receptors, ligands, enzymes, and phospholipids that play important roles in cell-cell and cell-extracellular matrix interactions governing, for instance, tissue development and repair. We previously showed that plasma membrane nanofragments (PMNFs) act as nucleation sites for bone formation in vivo, and induce in vitro mineralization within 1 day. In this study, we optimized the methods for generating, isolating, and applying PMNFs as a cell-free therapeutic to expedite bone defect repair. The PMNFs were isolated from different mouse cell lines (chondrocytes, osteoblasts, and fibroblasts), pre-conditioned, lyophilized, and subsequently transplanted into 2 mm critical-sized calvarial defects in mice (n = 75). The PMNFs from chondrocytes, following a 3-day pre-incubation, significantly accelerated bone repair within 2 weeks, through a coordinated attraction of macrophages, endothelial cells, and osteoblasts to the healing site. In vitro experiments confirmed that PMNFs enhanced cell adhesion. Comparison of the PMNF efficacy with phosphatidylserine, amorphous calcium phosphate (ACP), and living cells confirmed the unique ability of PMNFs to promote accelerated bone repair. Importantly, PMNFs promoted nearly complete integration of the regenerated bone with native tissue after 6 weeks (% non-integrated bone area = 15.02), contrasting with the partial integration (% non-integrated bone area = 56.10; p < 0.01, Student's test) with transplantation of ACP. Vickers microhardness tests demonstrated that the regenerated bone after 6 weeks (30.10 ± 1.75) exhibited hardness similar to native bone (31.07 ± 2.46). In conclusion, this is the first study to demonstrate that cell membrane can be a promising cell-free material with multifaceted biofunctional properties that promote accelerated bone repair. STATEMENT OF SIGNIFICANCE.

DOI： 10.1016/j.actbio.2024.07.037

PubMed

researchmap

Re-Evaluation of Initial Bone Mineralization from an Engineering Perspective 査読国際誌

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Tissue Engineering Part B: Reviews 28 ( 1 ) 246 - 255 2022年2月

Biohybrid Variable‐Stiffness Soft Actuators that Self‐Create Bone 査読

Danfeng Cao, Jose G. Martinez, Emilio Satoshi Hara, Edwin W. H. Jager

Advanced Materials 34 ( 8 ) 2107345 - 2107345 2022年2月

Effect of Biomechanical Environment on Degeneration of Meckel’s Cartilage 査読

Mahmoud Farahat, Gulsan A.S. Kazi, Emilio S. Hara, Takuya Matsumoto

Journal of Dental Research 100 ( 2 ) 171 - 178 2021年2月

Bone Marrow Cells Inhibit BMP‐2‐Induced Osteoblast Activity in the Marrow Environment 査読

Ha Thi Nguyen, Mitsuaki Ono, Yasutaka Oida, Emilio Satoshi Hara, Taishi Komori, Kentaro Akiyama, Ha Thi Thu Nguyen, Kyaw Thu Aung, Hai Thanh Pham, Ikue Tosa, Takeshi Takarada, Koichi Matsuo, Toshihide Mizoguchi, Toshitaka Oohashi, Takuo Kuboki

Journal of Bone and Mineral Research 34 ( 2 ) 327 - 332 2019年2月

Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu 査読

Emilio Satoshi Hara, Masahiro Okada, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

Journal of Materials Chemistry B 6 ( 38 ) 6153 - 6161 2018年9月

Bioinspired Mineralization Using Chondrocyte Membrane Nanofragments 査読国際誌

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

ACS Biomaterials Science & Engineering 4 ( 2 ) 617 - 625 2018年2月

Biocompatible nanostructured solid adhesives for biological soft tissues 査読

Masahiro Okada, Akira Nakai, Emilio Satoshi Hara, Tetsushi Taguchi, Takayoshi Nakano, Takuya Matsumoto

Acta Biomaterialia 57 404 - 413 2017年

Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF‐β3–Associated Chondrogenesis of Bone Marrow–Derived Mesenchymal Stem Cells for Articular Surface Regeneration 査読

Emilio Satoshi Hara, Mitsuaki Ono, Hai Thanh Pham, Wataru Sonoyama, Satoshi Kubota, Masaharu Takigawa, Takuya Matsumoto, Marian F Young, Bjorn R Olsen, Takuo Kuboki

Journal of Bone and Mineral Research 30 ( 9 ) 1585 - 1596 2015年9月

Novel chondrogenic and chondroprotective effects of the natural compound harmine 査読

Emilio Satoshi Hara, Mitsuaki Ono, Satoshi Kubota, Wataru Sonoyama, Yasutaka Oida, Takako Hattori, Takashi Nishida, Takayuki Furumatsu, Toshifumi Ozaki, Masaharu Takigawa, Takuo Kuboki

Biochimie 95 ( 2 ) 374 - 381 2013年2月

BMP-2 generates a functional bone marrow niche by inducing the differentiation of local mesenchymal cells into CAR cells

Ikue Tosa, Mitsuaki Ono, Anh Tuan Dang, Ziyi Wang, Maiko Kimura, Wakana Kitagawa, Kei Ishibashi, Hang Thuy Do, Kun Zhao, Emilio Satoshi Hara, Noboru Asada, Takashi Nagasawa, Toshitaka Oohashi, Naoya Ohara, Takuo Kuboki

Blood Advances 2025年3月

Local E-rhBMP-2/β-TCP Application Rescues Osteocyte Dendritic Integrity and Reduces Microstructural Damage in Alveolar Bone Post-Extraction in MRONJ-like Mouse Model 査読

Anh Tuan Dang, Mitsuaki Ono, Ziyi Wang, Ikue Tosa, Emilio Satoshi Hara, Akihiro Mikai, Wakana Kitagawa, Tomoko Yonezawa, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 25 ( 12 ) 6648 - 6648 2024年6月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

The pathology of medication-related osteonecrosis of the jaw (MRONJ), often associated with antiresorptive therapy, is still not fully understood. Osteocyte networks are known to play a critical role in maintaining bone homeostasis and repair, but the exact condition of these networks in MRONJ is unknown. On the other hand, the local application of E-coli-derived Recombinant Human Bone Morphogenetic Protein 2/β-Tricalcium phosphate (E-rhBMP-2/β-TCP) has been shown to promote bone regeneration and mitigate osteonecrosis in MRONJ-like mouse models, indicating its potential therapeutic application for the treatment of MRONJ. However, the detailed effect of BMP-2 treatment on restoring bone integrity, including its osteocyte network, in an MRONJ condition remains unclear. Therefore, in the present study, by applying a scanning electron microscope (SEM) analysis and a 3D osteocyte network reconstruction workflow on the alveolar bone surrounding the tooth extraction socket of an MRONJ-like mouse model, we examined the effectiveness of BMP-2/β-TCP therapy on the alleviation of MRONJ-related bone necrosis with a particular focus on the osteocyte network and alveolar bone microstructure (microcrack accumulation). The 3D osteocyte dendritic analysis showed a significant decrease in osteocyte dendritic parameters along with a delay in bone remodeling in the MRONJ group compared to the healthy counterpart. The SEM analysis also revealed a notable increase in the number of microcracks in the alveolar bone surface in the MRONJ group compared to the healthy group. In contrast, all of those parameters were restored in the E-rhBMP-2/β-TCP-treated group to levels that were almost similar to those in the healthy group. In summary, our study reveals that MRONJ induces osteocyte network degradation and microcrack accumulation, while application of E-rhBMP-2/β-TCP can restore a compromised osteocyte network and abrogate microcrack accumulation in MRONJ.

DOI： 10.3390/ijms25126648

researchmap

Electrochemical control of bone microstructure on electroactive surfaces for modulation of stem cells and bone tissue engineering 査読国際誌

Danfeng Cao, Jose G. Martinez, Risa Anada, Emilio Satoshi Hara, Hiroshi Kamioka, Edwin W. H. Jager

Science and Technology of Advanced Materials 24 ( 1 ) 2183710 - 2183710 2023年12月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Controlling stem cell behavior at the material interface is crucial for the development of novel technologies in stem cell biology and regenerative medicine. The composition and presentation of bio-factors on a surface strongly influence the activity of stem cells. Herein, we designed an electroactive surface that mimics the initial process of trabecular bone formation, by immobilizing chondrocyte-derived plasma membrane nanofragments (PMNFs) on its surface for rapid mineralization within 2 days. Moreover, the electroactive surface was based on the conducting polymer polypyrrole (PPy), which enabled dynamic control of the presentation of PMNFs on the surface via electrochemical redox switching, further resulting in the formation of bone minerals with different morphologies. Furthermore, bone minerals with contrasting surface morphologies had differential effects on the differentiation of human bone marrow-derived stem cells (hBMSCs) cultured on the surface. Together, this electroactive surface showed multifunctional characteristics, not only allowing dynamic control of PMNF presentation but also promoting the formation of bone minerals with different morphologies within 2 days. This electroactive substrate could be valuable for more precise control of stem cell growth and differentiation, and further development of more suitable microenvironments containing bone apatite for housing a bone marrow stem cell niche, such as biochips/bone-on-chips.

DOI： 10.1080/14686996.2023.2183710

PubMed

researchmap

Tunable electroactive biomimetic bone-like surfaces for bone marrow-on-chips 査読

Danfeng Cao, Jose G. Martinez, Laetitia Skalla, Erik Hultin, Jan-Ingvar Jönsson, Risa Anada, Hiroshi Kamioka, Edwin W. H. Jager, Emilio Satoshi Hara

2023 IEEE BioSensors Conference (BioSensors) 1 - 4 2023年7月

Variable Stiffness Actuators with Covalently Attached Nanofragments that Induce Mineralization 査読国際誌

Danfeng Cao, Jose G. Martinez, Emilio Satoshi Hara, Edwin W. H. Jager

Advanced Materials Technologies 2201651 - 2201651 2023年4月

Important roles of odontoblast membrane phospholipids in early dentin mineralization 査読国際誌

Risa Anada, Emilio Satoshi Hara, Noriyuki Nagaoka, Masahiro Okada, Hiroshi Kamioka, Takuya Matsumoto

Journal of Materials Chemistry B 11 ( 3 ) 657 - 666 2023年1月

Fabrication of initial trabecular bone inspired three-dimensional structure with cell membrane nanofragments 査読

Koichi Kadoya, Emilio Satoshi Hara, Masahiro Okada, YuYang Jiao, Takayoshi Nakano, Akira Sasaki, Takuya Matsumoto

2022年11月

Distinct Morphologies of Bone Apatite Clusters in Endochondral and Intramembranous Ossification 査読

Emilio Satoshi Hara, Noriyuki Nagaoka, Masahiro Okada, Takayoshi Nakano, Takuya Matsumoto

Advanced Biology 6 ( 11 ) 2200076 - 2200076 2022年11月

Soft actuators that self-create bone for biohybrid (micro)robotics 査読

Danfeng Cao, Jose G. Martinez, Emilio Satoshi Hara, Edwin W. H. Jager

2022 International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS) 2022年7月

Mechanotransductive Mechanisms of Biomimetic Hydrogel Cues Modulating Meckel's Cartilage Degeneration 査読

Mahmoud Farahat, Emilio S. Hara, Risa Anada, Gulsan A.S. Kazi, Nahid M. Akhter, Takuya Matsumoto

Advanced Biology 2101315 - 2101315 2022年6月

Self-adhering implantable device of titanium: Enhanced soft-tissue adhesion by sandblast pretreatment 査読

Atsushi Yabe, Masahiro Okada, Emilio Satoshi Hara, Yasuhiro Torii, Takuya Matsumoto

Colloids and Surfaces B: Biointerfaces 211 112283 - 112283 2022年3月

Suppression of Bone Necrosis around Tooth Extraction Socket in a MRONJ-like Mouse Model by E-rhBMP-2 Containing Artificial Bone Graft Administration 査読国際誌

Yukie Tanaka, Kyaw Thu Aung, Mitsuaki Ono, Akihiro Mikai, Anh Tuan Dang, Emilio Satoshi Hara, Ikue Tosa, Kei Ishibashi, Aya Ono-Kimura, Kumiko Nawachi, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 22 ( 23 ) 12823 - 12823 2021年11月

Immediate soft-tissue adhesion and the mechanical properties of the Ti–6Al–4V alloy after long-term acid treatment 査読国際誌

Yaming Wang, Masahiro Okada, Shi Chao Xie, Yu Yang Jiao, Emilio Satoshi Hara, Hiroaki Yanagimoto, Takumi Fukumoto, Takuya Matsumoto

Journal of Materials Chemistry B 9 ( 39 ) 8348 - 8354 2021年8月

Nanostructural analysis of distinct nucleation sites in pathological mineralization 査読

Emilio Satoshi Hara, Susumu Oozawa, Noriyuki Nagaoka, Masahiro Okada, Shingo Kasahara, Takuya Matsumoto

Materials Advances 2 ( 13 ) 4423 - 4431 2021年6月

Preclinical bioequivalence study of E.coli-derived rhBMP-2/β-TCP and autogenous bone in a canine guided-bone regeneration model. 査読

Shuji Nosho, Mitsuaki Ono, Taishi Komori, Akihiro Mikai, Ikue Tosa, Kei Ishibashi, Yukie Tanaka, Aya Kimura-Ono, Emilio S Hara, Toshitaka Oohashi, Takuo Kuboki

Journal of Prosthodontic Research 66 ( 1 ) 124 - 130 2021年6月

Tryptophan and Kynurenine Enhances the Stemness and Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro and In Vivo 査読

Hai Pham, Mitsuaki Ono, Emilio S. Hara, Ha Nguyen, Anh Dang, Hang Do, Taishi Komori, Ikue Tosa, Yuri Hazehara-Kunitomo, Yuya Yoshioka, Yasutaka Oida, Kentaro Akiyama, Takuo Kuboki

Materials 14 ( 1 ) 208 - 208 2021年1月

Micro-Architectural Investigation of Teleost Fish Rib Inducing Pliant Mechanical Property 査読国際誌

Yu Yang Jiao, Masahiro Okada, Emilio Satoshi Hara, Shi Chao Xie, Noriyuki Nagaoka, Takayoshi Nakano, Takuya Matsumoto

Materials 13 ( 22 ) 5099 - 5099 2020年11月

Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model 査読

Kyaw Thu Aung, Kentaro Akiyama, Masayoshi Kunitomo, Aung Ye Mun, Ikue Tosa, Ha Thi Thu Nguyen, Jiewen Zhang, Teisaku Kohno, Mitsuaki Ono, Emilio Satoshi Hara, Takuo Kuboki

International Journal of Molecular Sciences 21 ( 21 ) 8103 - 8103 2020年10月

Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments 査読

Shuji Nosho, Ikue Tosa, Mitsuaki Ono, Emilio Satoshi Hara, Kei Ishibashi, Akihiro Mikai, Yukie Tanaka, Aya Kimura-Ono, Taishi Komori, Kenji Maekawa, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 21 ( 21 ) 7967 - 7967 2020年10月

BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model 査読

Akihiro Mikai, Mitsuaki Ono, Ikue Tosa, Ha Thi Thu Nguyen, Emilio Satoshi Hara, Shuji Nosho, Aya Kimura-Ono, Kumiko Nawachi, Takeshi Takarada, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 21 ( 19 ) 7028 - 7028 2020年9月

Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis 査読

Mst Nahid Akhter, Emilio Hara, Koichi Kadoya, Masahiro Okada, Takuya Matsumoto

International Journal of Molecular Sciences 21 ( 15 ) 5327 - 5327 2020年7月

掲載種別：研究論文（学術雑誌）出版者・発行元：{MDPI} {AG}

Current stem cell-based techniques for bone-like tissue synthesis require at least two to three weeks. Therefore, novel techniques to promote rapid 3D bone-like tissue synthesis in vitro are still required. In this study, we explored the concept of using cell nanofragments as a substrate material to promote rapid bone formation in vitro. The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. The results showed that ultrasonication for 3 min allowed the fabrication of homogeneous nanofragments of less than 150 nm in length, which mineralized surprisingly in just one day, faster than the fragments obtained from all other methods. Further optimization of culture conditions indicated that a concentration of 10 mM or 100 mM of β-glycerophosphate enhanced, whereas fetal bovine serum (FBS) inhibited in a concentration-dependent manner, the mineralization of the cell nanofragments. Finally, a 3D collagen-cell nanofragment-mineral complex mimicking a bone-like structure was generated in just two days by combining the cell nanofragments in collagen gel. In conclusion, sonication for three min could be applied as a novel method to fabricate cell nanofragments of less than 150 nm in length, which can be used as a material for in vitro bone tissue engineering.

DOI： 10.3390/ijms21155327

researchmap

Titanium as an Instant Adhesive for Biological Soft Tissue 査読

Masahiro Okada, Emilio Satoshi Hara, Atsushi Yabe, Kei Okada, Yo Shibata, Yasuhiro Torii, Takayoshi Nakano, Takuya Matsumoto

Advanced Materials Interfaces 7 ( 9 ) 1902089 - 1902089 2020年5月

Association of masticatory muscle activity with sleep arousal and other concomitant movements during sleep 査読

Haruna Miki, Hajime Minakuchi, Mayu Miyagi, Emilio Satoshi Hara, Shuji Shigemoto, Yoshitaka Suzuki, Kenji Maekawa, Yoshizo Matsuka, Glenn T. Clark, Takuo Kuboki

Journal of Oral Rehabilitation 2020年3月

Vibratory splint therapy for decreasing sleep clenching: A pilot study 査読

Emilio Satoshi Hara, Andrea Lusvarghi Witzel, Hajime Minakuchi, Carlos Eduardo de Pitta, Rosane Tronquin Gallo, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Marcelo Costa Bolzan

CRANIO: The Journal of Craniomandibular & Sleep Practice 38 ( 1 ) 15 - 21 2020年1月

DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation 査読

Yu Nomura, Emilio Satoshi Hara, Yuya Yoshioka, Há Thi Nguyen, Shuji Nosho, Taishi Komori, Kei Ishibashi, Toshitaka Oohashi, Mitsuaki Ono, Takuo Kuboki

Cells Tissues Organs 207 ( 3-4 ) 115 - 126 2019年10月

担当区分：責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：S. Karger AG

Stem cells have essential applications in in vitro tissue engineering or regenerative medicine. However, there is still a need to understand more deeply the mechanisms of stem cell differentiation and to optimize the methods to control stem cell function. In this study, we first investigated the activity of DNA methyltransferases (DNMTs) during chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) and found that DNMT3A and DNMT3B were markedly upregulated during hBMSC chondrogenic differentiation. In an attempt to understand the effect of DNMT3A and DNMT3B on the chondrogenic differentiation of hBMSCs, we transiently transfected the cells with expression vectors for the two enzymes. Interestingly, DNMT3A overexpression strongly enhanced the chondrogenesis of hBMSCs, by increasing the gene expression of the mature chondrocyte marker, collagen type II, more than 200-fold. Analysis of the methylation condition in the cells revealed that DNMT3A and DNMT3B methylated the promoter sequence of early stem cell markers, NANOG and POU5F1(OCT-4). Conversely, the suppression of chondrogenic differentiation and the increase in stem cell markers of hBMSCs were obtained by chemical stimulation with the demethylating agent, 5-azacitidine. Loss-of-function assays with siRNAs targeting DNMT3A also significantly suppressed the chondrogenic differentiation of hBMSCs. Together, these results not only show the critical roles of DNMTs in regulating the chondrogenic differentiation of hBMSCs, but also suggest that manipulation of DNMT activity can be important tools to enhance the differentiation of hBMSCs towards chondrogenesis for potential application in cartilage tissue engineering or cartilage regeneration.

DOI： 10.1159/000502885

PubMed

researchmap

Type XVIII Collagen Modulates Keratohyalin Granule Formation and Keratinization in Oral Mucosa 査読

Ha Thi Thu Nguyen, Mitsuaki Ono, Emilio Hara, Taishi, Midori Edamatsu, Tomoko Yonezawa, Aya Kimura-Ono, Kenji Maekawa, Takuo Kuboki, Toshitaka Oohashi

International Journal of Molecular Sciences 20 ( 19 ) 4739 - 4739 2019年9月

Fibronectin-induced ductal formation in salivary gland self-organization model 査読

Mahmoud Farahat, Gulsan A. S. Kazi, Hiroaki Taketa, Emilio S. Hara, Masamitsu Oshima, Takuo Kuboki, Takuya Matsumoto

DEVELOPMENTAL DYNAMICS 248 ( 9 ) 813 - 825 2019年9月

Improvement of mechanical properties of Y-TZP by thermal annealing with monoclinic zirconia nanoparticle coating 査読国際誌

Masahiro Okada, Hiroaki Taketa, Emilio Satoshi Hara, Yasuhiro Torii, Masao Irie, Takuya Matsumoto

Dental Materials 35 ( 7 ) 970 - 978 2019年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier BV

OBJECTIVE: To assess whether a thermal annealing with a monoclinic zirconia (mZrO2) nanoparticle coating can improve the reliability of sandblasted yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) and maintain its mechanical strength. METHODS: Commercially available Y-TZP (Lava Frame, 3M Dental Products) disks were sintered and surface-treated as follows: AS (as sintered, with no treatment); SB (sandblasting); SB-TA (sandblasting followed by thermal annealing at 1000 °C); and SB-mZr-TA (sandblasting followed by thermal annealing at 1000 °C with the mZrO2 nanoparticle coating). The mZrO2 nanoparticles of 21 nm in size were prepared by a hydrothermal method, and coated onto Y-TZP sintered disks as a 5 g/L ethanol dispersion. Biaxial flexural strength (S) was measured using the piston-on-three-ball test, and reliability was evaluated by the Weibull modulus (m). RESULTS: Biaxial flexural tests showed a significant increase in the strength of Group SB (SSB = 1445 ± 191 MPa) compared with Group AS (SAS = 1071 ± 112 MPa). The thermal annealing improved the reliabilities of the sandblasted Y-TZP (mSB-TA = 20.14 and mSB-mZr-TA = 21.33), as compared with Group SB (mSB = 7.77). However, the conventional thermal annealing without the mZrO2 coating caused a significant decrease in the strength of sandblasted Y-TZP (SSB-TA = 1273 ± 65 MPa). Importantly, the mZrO2 coating prevented the decrease in the strength caused by conventional thermal annealing (SSB-mZr-TA = 1379 ± 65 MPa). SIGNIFICANCE: The thermal annealing with the mZrO2 nanoparticle coating can improve the reliability of sandblasted Y-TZP and maintain its mechanical strength, which would otherwise be decreased by the conventional annealing process.

DOI： 10.1016/j.dental.2019.04.002

PubMed

researchmap

Biomimetic mineralization using matrix vesicle nanofragments 査読

Yosuke Kunitomi, Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takuo Kuboki, Takayoshi Nakano, Hiroshi Kamioka, Takuya Matsumoto

Journal of Biomedical Materials Research Part A 107 ( 5 ) 1021 - 1030 2019年5月

Intermediate Water on Calcium Phosphate Minerals: Its Origin and Role in Crystal Growth 査読

Masahiro Okada, Emilio Satoshi Hara, Daisuke Kobayashi, Shoki Kai, Keiko Ogura, Masaru Tanaka, Takuya Matsumoto

ACS Applied Bio Materials 2019年3月

Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells 査読

Yuri Hazehara-Kunitomo, Emilio S. Hara, Mitsuaki Ono, Kyaw Aung, Keiko Komi, Hai Pham, Kentaro Akiyama, Masahiro Okada, Toshitaka Oohashi, Takuya Matsumoto, Takuo Kuboki

International Journal of Molecular Sciences 20 ( 5 ) 1097 - 1097 2019年3月

骨組織発生の材料学的再検討が新しい骨組織工学につながる

松本卓也, HARA ES

バイオマテリアル 37 ( 1 ) 50 - 51 2019年

New Functions of Classical Compounds against Orofacial Inflammatory Lesions 査読

Norifumi Moritani, Emilio Hara, Satoshi Kubota

Medicines 5 ( 4 ) 118 - 118 2018年11月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Anti-inflammatory agents have been widely used to ameliorate severe inflammatory symptoms of a number of diseases, and such therapeutics are particularly useful for diseases with intolerable pain without significant mortality. A typical example of this is a disease known as stomatitis; although stomatitis itself is not a life-threatening disease, it severely impairs the individual’s quality of life, and thus a standard therapeutic strategy for it has already been established. The topical application of a bioactive agent is quite easy, and a strong anti-inflammatory agent can be used without significant adverse effects. In contrast, natural products with relatively mild bioactivity are used for systemic intervention. However, new aspects of classical drugs used in these established therapeutic methods have recently been discovered, which is expanding the utility of these compounds to other oral diseases such as osteoarthritis of temporomandibular joints (TMJ-OA). In this review article, after summarizing the general concept and pathobiology of stomatitis, its established therapeutics are explained. Thereafter, recent advances in the research into related compounds, which is uncovering new biological functions of the agents used therein, are introduced. Indeed, regenerative therapeutics for TMJ-OA may be developed with the classical compounds currently being used.

DOI： 10.3390/medicines5040118

researchmap

CCN4/WISP1 controls cutaneous wound healing by modulating proliferation, migration and ECM expression in dermal fibroblasts via α5β1 and TNFα 査読

Mitsuaki Ono, Asuka Masaki, Azusa Maeda, Tina M. Kilts, Emilio S. Hara, Taishi Komori, Hai Pham, Takuo Kuboki, Marian F. Young

Matrix Biology 68-69 533 - 546 2018年8月

Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium 査読

Taishi Komori, Mitsuaki Ono, Emilio Satoshi Hara, Junji Ueda, Ha Thi Thu Nguyen, Ha Thi Nguyen, Tomoko Yonezawa, Takahiro Maeba, Aya Kimura-Ono, Takeshi Takarada, Ryusuke Momota, Kenji Maekawa, Takuo Kuboki, Toshitaka Oohashi

Scientific Reports 8 ( 1 ) 2018年2月

Fabrication of Hydroxyapatite Nanofibers with High Aspect Ratio via Low-Temperature Wet Precipitation Methods Under Acidic Conditions: From Molecular and Nano-structural Analyses to Environmental Science 査読

Emilio Hara

Biomineralization 211 - 218 2018年1月

Chondrocyte burst promotes space for mineral expansion 査読

Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Letycia Mary Iida, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto

Integrative Biology 10 ( 1 ) 57 - 66 2018年1月

差初期石灰化過程を詳細に観察し模倣する

松本卓也, HARA ES

化学工業 69 ( 6 ) 23 - 28 2018年

MCSF orchestrates branching morphogenesis in developing submandibular gland tissue 査読

Gulsan Ara Sathi, Mahmoud Farahat, Emilio Satoshi Hara, Hiroaki Taketa, Hitoshi Nagatsuka, Takuo Kuboki, Takuya Matsumoto

Journal of Cell Science 130 ( 9 ) 1559 - 1569 2017年

Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model 査読

Mitsuaki Ono, Masamitsu Oshima, Miho Ogawa, Wataru Sonoyama, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Ryu Nakajima, Atsushi Mine, Satoru Hayano, Satoshi Fukumoto, Shohei Kasugai, Akira Yamaguchi, Takashi Tsuji, Takuo Kuboki

Scientific Reports 7 44522 - 44522 2017年

MSCs feeder layers induce SMG self-organization and branching morphogenesis 査読

Mahmoud Farahat, Gulsan Ara Sathi, Emilio Satoshi Hara, Hiroaki Taketa, Takuo Kuboki, Takuya Matsumoto

PLOS ONE 12 ( 4 ) e0176453 2017年

皮膚創傷治癒過程におけるCCN4/WISP-1の役割

大野充昭, 正木明日香, 前田あずさ, Hara Emilio S., 小盛大志, 久保田聡, Young Marian F., 大橋俊孝, 窪木拓男

日本結合組織学会学術大会プログラム・抄録集 48回 88 - 88 2016年6月

Assessment of CCN2 Independent Modules Regenerative Capacity on Osteoarthritis and Further Selecting the Most Suitable Among them as a Potential Therapeutic Drug 査読

Tarek Abdelkader, Eriko Aoyama, Takashi Nishida, Takako Hattori, Danilo Janune, Emilio S. Hara, Mitsuaki Ono, Yasuhiko Tabata, Takuo Kuboki, Satoshi Kubota, Masaharu Takigawa

FASEB JOURNAL 30 2016年4月

Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects 査読

Minakuchi, H., Sogawa, C., Miki, H., Hara, E.S., Maekawa, K., Sogawa, N., Kitayama, S., Matsuka, Y., Clark, G.T., Kuboki, T.

Sleep and Breathing 20 ( 1 ) 271 - 276 2016年3月

Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects 査読

Hajime Minakuchi, Chiharu Sogawa, Haruna Miki, Emilio S. Hara, Kenji Maekawa, Norio Sogawa, Shigeo Kitayama, Yoshizo Matsuka, Glenn Thomas Clark, Takuo Kuboki

SLEEP AND BREATHING 20 ( 1 ) 271 - 276 2016年3月

CCN4/WISP-1 positively regulates chondrogenesis by controlling TGF-beta 3 function 査読

Yoshioka Yuya, Ono Mitsuaki, Maeda Azusa, Kilts Tina M, Hara Emilio Satoshi, Khattab Hany, Ueda Junji, Aoyama Eriko, Oohashi Toshitaka, Takigawa Masaharu, Young Marian F, Kuboki Takuo

Bone 83 162 - 170 2016年

Antagonistic Effects of Insulin and TGF-β3 during Chondrogenic Differentiation of Human BMSCs under a Minimal Amount of Factors 査読

Emilio Satoshi Hara, Mitsuaki Ono, Yuya Yoshioka, Junji Ueda, Yuri Hazehara, Hai Thanh Pham, Takuya Matsumoto, Takuo Kuboki

Cells Tissues Organs 201 ( 2 ) 88 - 96 2016年

Fluocinolone acetonideとTGF-β3を用いた強力な軟骨再生(Potent cartilage regeneration with fluocinolone acetonide and TGF-β3)

Hara Emilio Satoshi, 大野充昭, Pham Hai Thanh, 久保田聡, 滝川正春, Young Marian F., Olsen Bjorn R., 松本卓也, 窪木拓男

日本バイオマテリアル学会大会予稿集 37回 209 - 209 2015年11月

CCN4はTGF-β3の機能を制御することで軟骨細胞分化を正に制御する

吉岡裕也, 大野充昭, 前田あずさ, Hara Emilio Satoshi, Young Marian, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 33回 216 - 216 2015年7月

糖質コルチコイドの中でfluocinolone acetonideはTGF-β3介在性BMSCの軟骨形成の活性化と関節の軟骨修復を促進において特異的な作用を有する(Among glucocorticoids, fluocinolone acetonide is unique in potentiating TGF-β 3-mediated chondrogenesis of BMSCs and promoting articular cartilage repair)

Hara Emilio Satoshi, 大野充昭, Pham Hai Thanh, Young Marian F., 久保田聡, 滝川正春, 窪木拓男

日本骨代謝学会学術集会プログラム抄録集 33回 161 - 161 2015年7月

A longitudinal retrospective study of the analysis of the risk factors of implant failure by the application of generalized estimating equations 査読

Noda Kinji, Arakawa Hikaru, Kimura-Ono Aya, Yamazaki Seiya, Hara Emilio Satoshi, Sonoyama Wataru, Maekawa Kenji, Okura Kazuo, Shintani Ayumi, Matsuka Yoshizo, Kuboki Takuo

Journal of Prosthodontic Research 59 ( 3 ) 178 - 184 2015年

In silico comparison of the reproducibility of full-arch implant provisional restorations to final restoration between a 3D Scan/CAD/CAM technique and the conventional method 査読

Mino Takuya, Maekawa Kenji, Ueda Akihiro, Higuchi Shizuo, Sejima Junichi, Takeuchi Tetsuo, Hara Emilio Satoshi, Kimura-Ono Aya, Sonoyama Wataru, Kuboki Takuo

Journal of Prosthodontic Research 59 ( 2 ) 152 - 158 2015年

OstemiR: A Novel Panel of MicroRNA Biomarkers in Osteoblastic and Osteocytic Differentiation from Mesencymal Stem Cells 査読

Eguchi Takanori, Hara Emilio Satoshi, Ono Mitsuaki, Watanabe Ken, Kuboki Takuo, Calderwood Stuart

Faseb Journal 29 2015年

A risk factor analysis of accumulated postoperative pain and swelling sensation after dental implant surgery using a cellular phone-based real-time assessment 査読

Kuroi R, Minakuchi H, Hara E.S, Kawakami A, Maekawa K, Okada H, Kuboki T

Journal of Prosthodontic Research 59 ( 3 ) 194 - 198 2015年

炎症環境による歯髄細胞の幹細胞化歯髄細胞分化に与えるTNF-αの影響

上枝麻友, 藤澤拓生, 大野充昭, Hara Emilio Satoshi, Pham Thanh Hai, 園山亘, 窪木拓男, 松香芳三

日本再生歯科医学会誌 12 ( 1 ) 48 - 48 2014年12月

出生後のイヌ永久歯胚組織を用いた器官原基法による完全な臓器としての歯の再生

大野充昭, 大島正充, 園山亘, 小川美帆, 笈田育尚, Hara Emilio S., 新川重彦, 中島隆, 辻孝, 窪木拓男

日本補綴歯科学会誌 6 ( 特別号 ) 103 - 103 2014年5月

A short-term treatment with tumor necrosis factor-alpha enhances stem cell phenotype of human dental pulp cells 査読

Mayu Ueda, Takuo Fujisawa, Mitsuaki Ono, Emilio Satoshi Hara, Hai Thanh Pham, Ryu Nakajima, Wataru Sonoyama, Takuo Kuboki

Stem Cell Research & Therapy 5 ( 1 ) 2014年3月

The regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarthritis models in vivo 査読

Tarek Abd El Kader, Satoshi Kubota, Takashi Nishida, Takako Hattori, Eriko Aoyama, Danilo Janune, Emilio S. Hara, Mitsuaki Ono, Yasuhiko Tabata, Takuo Kuboki, Masaharu Takigawa

Bone 59 180 - 188 2014年2月

Mesenchymal Stem/Progenitor Cell Isolation from Tooth Extraction Sockets 査読

Nakajima R, Ono M, Hara E. S, Oida Y, Shinkawa S, Pham H. T, Akiyama K, Sonoyama W, Maekawa K, Kuboki T

Journal of Dental Research 93 ( 11 ) 1133 - 1140 2014年

miRNA-720 regulates the stem cell phenotype and differentiation of human dental pulp-derived mesenchymal stromal cells 査読

Emilio Satoshi Hara, Mitsuaki Ono, Takanori Eguchi, Hai Thanh Pham, Shoji Tajima, Stuart K. Calderwood, Takuya Matsumoto, Takuo Kuboki

2014 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS) 2014年

Regeneration of Calvarial Defects with Escherichia coli-Derived rhBMP-2 Adsorbed in PLGA Membrane 査読

Ono Mitsuaki, Sonoyama Wataru, Nema Kazuki, Hara Emilio Satoshi, Oida Yasutaka, Hai Thanh Pham, Yamamoto Katushi, Hirota Kazuo, Sugama Kazushige, Sebald Walter, Kuboki Takuo

Cells Tissues Organs 198 ( 5 ) 367 - 376 2014年

Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control 査読

Minakuchi Hajime, Sogawa Chiharu, Hara Emilio Satoshi, Miki Haruna, Maekawa Kenji, Sogawa Norio, Kitayama Shigeo, Matsuka Yoshizo, Clark Glenn T, Kuboki Takuo

Journal of Prosthodontic Research 58 ( 4 ) 217 - 222 2014年

Efficient Bone Formation in a Swine Socket Lift Model Using Escherichia coli-Derived Recombinant Human Bone Morphogenetic Protein-2 Adsorbed in beta-Tricalcium Phosphate 査読

Ono Mitsuaki, Sonoyama Wataru, Yamamoto Katushi, Oida Yasutaka, Akiyama Kentaro, Shinkawa Shigehiko, Nakajima Ryu, Pham Hai T, Hara Emilio S, Kuboki Takuo

Cells Tissues Organs 199 ( 4 ) 249 - 255 2014年

Immunotherapy in Transplantation 査読

Akiyama, K., Hara Satoshi, E., Kuboki, T.

Stem Cell Biology and Tissue Engineering in Dental Sciences 2014年

未分化性維持に関与しているmiRNA-720は歯髄細胞の細胞増殖・分化を制御する

Emilio Hara, 大野充昭, Hai Pham, 窪木拓男

Journal of Oral Biosciences Supplement 2013 155 - 155 2013年9月

抜歯窩肉芽組織からの新規間葉系幹細胞の同定

中島隆, 大野充昭, 園山亘, 笈田育尚, Hara Emilio S., 前川賢治, 窪木拓男

日本補綴歯科学会誌 5 ( 特別号 ) 112 - 112 2013年5月

皮膚創傷治癒過程におけるCCN4/WISP-1遺伝子の役割

正木明日香, 大野充昭, 園山亘, Hara Emilio S., 前田あずさ, Young Marian F., 窪木拓男

日本補綴歯科学会誌 5 ( 特別号 ) 227 - 227 2013年5月

A novel vibratory stimulation-based occlusal splint for alleviation of TMD painful symptoms: A pilot study 査読

Hara, E.S., Witzel, A.L., de Luca, C.E.P., Ballester, R.Y., Kuboki, T., Bolzan, M.C.

Journal of Oral Rehabilitation 40 ( 3 ) 179 - 184 2013年3月

A novel vibratory stimulation-based splint for chronic and untreatable masticatory myofascial pain: A case-series 査読

Hara, E.S., Witzel, A.L., de Luca, C.E.P., Ballester, R.Y., Bolzan, M.C.

Journal of Prosthodontic Research 57 ( 1 ) 62 - 66 2013年1月

OstemiR: A Novel Panel of MicroRNA Biomarkers in Osteoblastic and Osteocytic Differentiation from Mesencymal Stem Cells 査読

Eguchi Takanori, Watanabe Ken, Hara Emilio Satoshi, Ono Mitsuaki, Kuboki Takuo, Calderwood Stuart K

Plos One 8 ( 3 ) 2013年

A retrospective comparative ten-year study of cumulative survival rates of remaining teeth in large edentulism treated with implant-supported fixed partial dentures or removable partial dentures 査読

Yamazaki Seiya, Arakawa Hikaru, Maekawa Kenji, Hara Emilio Satoshi, Noda Kinji, Minakuchi Hajime, Sonoyama Wataru, Matsuka Yoshizo, Kuboki Takuo

Journal of Prosthodontic Research 57 ( 3 ) 156 - 161 2013年

Identification of risk factors for fracture of veneering materials and screw loosening of implant-supported fixed partial dentures in partially edentulous cases 査読

Noda K, Arakawa H, Maekawa K, Hara E. S, Yamazaki S, Kimura-Ono A, Sonoyama W, Minakuchi H, Matsuka Y, Kuboki T

Journal of Oral Rehabilitation 40 ( 3 ) 214 - 220 2013年

miRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cells 査読

Emilio Satoshi Hara, Mitsuaki Ono, Takanori Eguchi, Satoshi Kubota, Hai Thanh Pham, Wataru Sonoyama, Shoji Tajima, Masaharu Takigawa, Stuart K Calderwood, Takuo Kuboki

Plos One 8 ( 12 ) 2013年

Retrospective investigation of the remaining teeth status of patients with implant-supported fixed partial dentures in unilateral free-end edentulism 査読

Yamazaki Seiya, Arakawa Hikaru, Maekawa Kenji, Hara Emilio Satoshi, Noda Kinji, Minakuchi Hajime, Sonoyama Wataru, Matsuka Yoshizo, Kuboki Takuo

Journal of Prosthodontic Research 57 ( 4 ) 262 - 267 2013年

A Retrospective Comparative 8-Year Study of Cumulative Complications in Teeth Adjacent to Both Natural and Implant-Supported Fixed Partial Dentures 査読

Yamazaki Seiya, Arakawa Hikaru, Maekawa Kenji, Noda Kinji, Hara Emilio Satoshi, Minakuchi Hajime, Sonoyama Wataru, Matsuka Yoshizo, Kuboki Takuo

International Journal of Prosthodontics 26 ( 3 ) 260 - 264 2013年

Multiple sleep bruxism data collected using a self-contained EMG detector/analyzer system in asymptomatic healthy subjects 査読

Hajime Minakuchi, Chiyomi Sakaguchi, Emilio S. Hara, Kenji Maekawa, Yoshizo Matsuka, Glenn T. Clark, Takuo Kuboki

Sleep and Breathing 16 ( 4 ) 1069 - 1072 2012年12月

Matrix metalloproteinase-8 is the major potential collagenase in active peri-implantitis 査読

Hikaru Arakawa, Junji Uehara, Emilio Satoshi Hara, Wataru Sonoyama, Aya Kimura, Manabu Kanyama, Yoshizo Matsuka, Takuo Kuboki

Journal of Prosthodontic Research 56 ( 4 ) 249 - 255 2012年10月

Occlusal dysesthesia: A qualitative systematic review of the epidemiology, aetiology and management 査読

Hara, E.S., Matsuka, Y., Minakuchi, H., Clark, G.T., Kuboki, T.

Journal of Oral Rehabilitation 39 ( 8 ) 630 - 638 2012年8月

大腸菌由来BMP-2を応用した骨再生療法の開発と評価

園山亘, 大野充昭, 笈田育尚, Hara Emilio Satoshi, 新川重彦, 中島隆, 正木明日香, 松香芳三, 山本克史, 広田一男, 洲鎌和茂, 窪木拓男

日本歯科医師会雑誌 65 ( 5 ) 678 - 678 2012年8月

睡眠時ブラキシズム頻度と末梢セロトニントランスポーター輸送能との関連

水口一, 三木春奈, 十川千春, Hara Emilio Satoshi, 藤澤拓生, 前川賢治, 松香芳三, 十川紀夫, 北山滋雄, 窪木拓男

日本補綴歯科学会誌 4 ( 特別号 ) 213 - 213 2012年5月

Response shift in oral health-related quality of life measurement in patients with partial edentulism 査読

Kimura, A., Arakawa, H., Noda, K., Yamazaki, S., Hara, E.S., Mino, T., Matsuka, Y., Mulligan, R., Kuboki, T.

Journal of Oral Rehabilitation 39 ( 1 ) 44 - 54 2012年1月

A problem-based learning tutorial for dental students regarding elderly residents in a nursing home in Japan 査読

Matsuka, Y., Nakajima, R., Miki, H., Kimura, A., Kanyama, M., Minakuchi, H., Shinkawa, S., Takiuchi, H., Nawachi, K., Maekawa, K., Arakawa, H., Fujisawa, T., Sonoyama, W., Mine, A., Hara, E.S., Kikutani, T., Kuboki, T.

Journal of Dental Education 76 ( 12 ) 1580 - 1588 2012年

The association between oral health-related quality of life and self-rated presenteeism 査読

Takiuchi Hiroya, Kimura-Ono Aya, Arakawa Hikaru, Mino Takuya, Masaki Asuka, Hara Emilio Satoshi, Kanyama Manabu, Matsuka Yoshizo, Kuboki Takuo

Quality of Life Research 21 71 - 72 2012年

睡眠時ブラキシズム発症頻度と末梢セロトニントランスポーター機能

水口一, ハラエミリオ, 三木春奈, 藤澤拓生, 前川賢治, 松香芳三, 窪木拓男

日本顎関節学会雑誌 23 ( Suppl. ) 115 - 115 2011年7月

RELATIONSHIP BETWEEN SLEEP BRUXISM FREQUENCY AND UPTAKE ABILITY OF HUMAN PERIPHERAL SEROTONIN TRANSPORTER

Minakuchi, H., Hara, E. S., Sogawa, C., Miki, H., Fujisawa, T., Maekawa, K., Matsuka, Y., Sogawa, N., Kitayama, S., Kuboki, T.

Sleep 34 2011年

A 15-year Clinical Comparative Study of the Cumulative Survival Rate of Cast Metal Core and Resin Core Restorations Luted with Adhesive Resin Cement 査読

Hikasa Takayuki, Matsuka Yoshizo, Mine Atsushi, Minakuchi Hajime, Hara Emilio Satoshi, Van Meerbeek Bart, Yatani Hirofumi, Kuboki Takuo

International Journal of Prosthodontics 23 ( 5 ) 397 - 405 2010年

Influence of unilateral posterior disocclusion on the body balance of rats 査読国際誌

REVISTA DE ODONTOLOGIA DA UNICID 2008年

　前のページ - 次のページ　

<HARA EMILIO SATOSHI>

論文 - HARA EMILIO SATOSHI