研究者詳細 - 柳　文修

Efficacy of Cisplatin–CXCR4 Antagonist Combination Therapy in Oral Cancer 査読

Magnetic Resonance Imaging Signal Intensity in the Characterization of Odontogenic Lesions: A Systematic Review 査読

Munhoz Luciana, Okada Shunsuke, Hisatomi Miki, Fujita Mariko, Shimizu Yudai, Yanagi Yoshinobu, Arita Emiko Saito, Asaumi Jun-Ich

International Journal of Radiology and Imaging Technology 10 ( 1 ) 2024年6月

Saori Yoshida, Hotaka Kawai, Yamin Soe, Htoo Shwe Eain, Sho Sanou, Kiyofumi Takabatake, Yohei Takeshita, Miki Hisatomi, Hitoshi Nagatsuka, Junichi Asaumi, Yoshinobu Yanagi

Cancers 16 ( 13 ) 2326 - 2326 2024年6月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Cisplatin is a platinum-based compound that is widely used for treating inoperable oral squamous cell carcinoma (OSCC) in Japan; however, resistance to cisplatin presents a challenge and innovative approaches are required. We aimed to investigate the therapeutic potential of targeting the chemokine receptor CXCR4, which is involved in angiogenesis and tumor progression, using the CXCR4 inhibitor AMD3100, in combination with cisplatin. AMD3100 induced necrosis and bleeding in OSCC xenografts by inhibiting angiogenesis. We investigated the combined ability of AMD3100 plus cisplatin to enhance the antitumor effect in cisplatin-resistant OSCC. An MTS assay identified HSC-2 cells as cisplatin-resistant cells in vitro. Mice treated with the cisplatin-AMD combination exhibited the most significant reduction in tumor volume, accompanied by extensive hemorrhage and necrosis. Histological examination indicated thin and short tumor vessels in the AMD and cisplatin–AMD groups. These results indicated that cisplatin and AMD3100 had synergistic antitumor effects, highlighting their potential for vascular therapy of refractory OSCC. Antitumor vascular therapy using cisplatin combined with a CXCR4 inhibitor provides a novel strategy for addressing cisplatin-resistant OSCC.

DOI： 10.3390/cancers16132326

Are computed tomography images of the mandible useful in age and sex determination? A forensic science meta-analysis 査読

L. Munhoz, S. Okada, M. Hisatomi, Y. Yanagi, E. S. Arita, J. Asaumi

The Journal of forensic odonto-stomatology 42 ( 1 ) 38 - 57 2024年4月

掲載種別：研究論文（学術雑誌）

OBJECTIVES: This meta-analysis addresses the use of mandibular computed tomography (CT) scans for age and/or sex determination in forensic science. METHODS: Six databases were searched until June 2023, using the keyword "mandible" combined with keywords related to "multislice computed tomography" (MCT) or "cone-beam computed tomography" (CBCT) and keywords related to "skeletal age determination" or "sex determination analysis." MAIN RESULTS: Among the 23 studies included, 11 used MCT and 12 used CBCT to perform forensic assessments. Age determination was the aim of a single study, sex and agedeterminations were the objective of five studies, and the other studies investigated the determination of sex only. Metaanalysis could be performed only for sex determination. CONCLUSIONS: Mandible measurements are useful in sex determination, as the bicondylar and bigonial breadth are larger in males than in females. For the mandible angle, the meta-analysis results confirm sex dimorphism in CBCT scans but not in MCT scans. For age estimation, further studies are needed to prove that the mandible hole is a reliable parameter for age estimation. PROSPERO registration number: CRD42021260967.

DOI： 10.5281/zenodo.11058169

Imaging characteristics of incidentally detected cosmetic surgery-derived foreign bodies on CT images in the maxillofacial region 査読

Miki Hisatomi, Yohei Takeshita, Yoshinobu Yanagi, Shunsuke Okada, Mamiko Fujikura, Suzuka Yoshida, Toshiyuki Kawazu, Junichi Asaumi

Oral Radiology 40 ( 2 ) 277 - 284 2024年1月

掲載種別：研究論文（学術雑誌）出版者・発行元：Springer Science and Business Media LLC

Abstract

Objectives

This study examined the imaging characteristics of cosmetic surgery-derived foreign bodies in the maxillofacial region through a retrospective review of cosmetic material foreign bodies incidentally detected on computed tomography (CT) images in routine clinical practice.

Methods

We retrospectively investigated cases of cosmetic surgery-derived foreign bodies other than dental materials in the maxillofacial region, using 5 years of CT image data stored on an imaging server. The imaging findings of these foreign bodies were investigated, along with patient age, patient sex, whether the foreign bodies were associated with the disease targeted by the CT scan, and the availability of cosmetic surgery information prior to examination.

Results

Foreign bodies were more common in women (19/21 cases), and affected patients displayed a wide age range (20–84 years). Four types of cosmetic surgery-derived foreign bodies in the maxillofacial region were detected by CT examination: nasal prostheses (nasal region), lifting sutures and injectable facial fillers (both in the buccal region), and silicone chin implants (chin region).

Conclusions

A cosmetic surgery-derived foreign body should be suspected when a foreign body is identified without a dental source of infection. In addition, cosmetic surgery-derived foreign bodies may be present in numerous patients, regardless of age or sex.

DOI： 10.1007/s11282-023-00734-2

Characteristic Mean Kurtosis Values in Simple Diffusion Kurtosis Imaging of Dentigerous Cysts 査読

その他リンク： https://link.springer.com/article/10.1007/s11282-023-00734-2/fulltext.html

Yuka Fukumura, Masahiro Kuroda, Suzuka Yoshida, Yoshihide Nakamura, Yuki Nakamitsu, Wlla E. Al-Hammad, Kazuhiro Kuroda, Ryo Kamizaki, Yudai Shimizu, Yoshinori Tanabe, Kohei Sugimoto, Masataka Oita, Irfan Sugianto, Majd Barham, Nouha Tekiki, Nurul N. Kamaruddin, Yoshinobu Yanagi, Junichi Asaumi

Diagnostics 13 ( 24 ) 3619 - 3619 2023年12月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

We evaluated the usefulness of simple diffusion kurtosis (SD) imaging, which was developed to generate diffusion kurtosis images simultaneously with an apparent diffusion coefficient (ADC) map for 27 cystic disease lesions in the head and neck region. The mean kurtosis (MK) and ADC values were calculated for the cystic space. The MK values were dentigerous cyst (DC): 0.74, odontogenic keratocyst (OKC): 0.86, ranula (R): 0.13, and mucous cyst (M): 0, and the ADC values were DC: 1364 × 10−6 mm2/s, OKC: 925 × 10−6 mm2/s, R: 2718 × 10−6 mm2/s, and M: 2686 × 10−6 mm2/s. The MK values of DC and OKC were significantly higher than those of R and M, whereas their ADC values were significantly lower. One reason for the characteristic signal values in diffusion-weighted images of DC may be related to content components such as fibrous tissue and exudate cells. When imaging cystic disease in the head and neck region using SD imaging, the maximum b-value setting at the time of imaging should be limited to approximately 1200 s/mm2 for accurate MK value calculation. This study is the first to show that the MK values of DC are characteristically higher than those of other cysts.

DOI： 10.3390/diagnostics13243619

Difficult Diagnosis of Osteosarcoma of the mandible: A Case Report Comparing Pathology and Imaging Findings 査読

Fujikura M, Kawazu T, Kawai H, Okada S, Yanagi Y, Asaumi J

岡山歯学会雑誌 42 ( 2 ) 45 - 54 2023年12月

An imaging‑based diagnostic approach to vascular anomalies of the oral and maxillofacial region 査読

Wlla Al‑Hammad, Mamiko Fujikura, Miki Hisatomi, Shunsuke Okada, Luciana Munhoz, Toshiyuki Kawazu, Yohei Takeshita, Mariko Fujita, Yoshinobu Yanagi, Jun-Ichi Asaumi

Oncology Letters 26 ( 3 ) 2023年7月

Usefulness of Simple Diffusion Kurtosis Imaging for Head and Neck Tumors: An Early Clinical Study. 査読

Yudai Shimizu, Masahiro Kuroda, Yuki Nakamitsu, Wlla E Al-Hammad, Suzuka Yoshida, Yuka Fukumura, Yoshihide Nakamura, Kazuhiro Kuroda, Ryo Kamizaki, Satoshi Imajoh, Yoshinori Tanabe, Kohei Sugimoto, Masataka Oita, Irfan Sugianto, Babatunde O Bamgbose, Yoshinobu Yanagi, Junichi Asaumi

Acta medica Okayama 77 ( 3 ) 273 - 280 2023年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

Diffusion kurtosis (DK) imaging (DKI), a type of restricted diffusion-weighted imaging, has been reported to be useful for tumor diagnoses in clinical studies. We developed a software program to simultaneously create DK images with apparent diffusion coefficient (ADC) maps and conducted an initial clinical study. Multi-shot echo-planar diffusion-weighted images were obtained at b-values of 0, 400, and 800 sec/mm2 for simple DKI, and DK images were created simultaneously with the ADC map. The usefulness of the DK image and ADC map was evaluated using a pixel analysis of all pixels and a median analysis of the pixels of each case. Tumor and normal tissues differed significantly in both pixel and median analyses. In the pixel analysis, the area under the curve was 0.64 for the mean kurtosis (MK) value and 0.77 for the ADC value. In the median analysis, the MK value was 0.74, and the ADC value was 0.75. The MK and ADC values correlated moderately in the pixel analysis and strongly in the median analysis. Our simple DKI system created DK images simultaneously with ADC maps, and the obtained MK and ADC values were useful for differentiating head and neck tumors from normal tissue.

DOI： 10.18926/AMO/65492

分化度が異なるOSCCにおけるCXCR4阻害剤の有用性

吉田沙織, 河合穂高, 竹下洋平, 岡田俊輔, 藤倉満美子, 久富美紀, 河津俊幸, 長塚仁, 浅海淳一, 柳文修

日本病理学会会誌 112 ( 1 ) 290 - 290 2023年3月

分化度の異なる口腔扁平上皮癌におけるシスプラチンとCXCR4阻害剤併用の効果

吉田沙織, 河合穂高, 竹下洋平, 岡田俊輔, 藤倉満美子, 久富美紀, 河津俊幸, 長塚仁, 浅海淳一, 柳文修

日本口腔診断学会雑誌 36 ( 1 ) 82 - 82 2023年2月

Ultrasound procedure for the diagnosis of mass lesions in the oral region. 査読

Yohei Takeshita, Toshiyuki Kawazu, Miki Hisatomi, Shunsuke Okada, Mamiko Fujikura, Saori Yoshida, Yuri Namba, Yudai Shimizu, Yoshinobu Yanagi, Junichi Asaumi

Oral radiology 2023年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

OBJECTIVES: To examine the diagnostic usefulness and procedures of ultrasonography (US) for mass lesions in the soft tissue of the oral region. METHODS: This study involved patients with mass lesions (tumorous lesions and cysts) who had undergone US and histopathological examinations from January 2017 to December 2019. The following points were evaluated by two observers using an evaluation scale: vascularity, echo intensity level, boundary, margin shape, distribution of internal echoes, and capsule. The usefulness of each point for differential diagnosis of tumorous lesions and cysts was statistically analyzed. RESULTS: Forty-five mass lesions in the soft tissue of the oral region (33 tumorous lesions and 12 cysts) were analyzed. There were significant differences in four evaluation points between the tumorous lesions and cysts: vascularity, echo intensity level, boundary, and margin shape. Cysts were almost completely excluded diagnostically, especially when vascularity was observed. There were also significant differences in two evaluation points between nonvascular tumorous lesions and cysts: echo intensity level and boundary. CONCLUSIONS: In US examination for mass lesions in the oral region, it was possible to diagnose tumorous lesions and exclude cysts when vascularity was observed. When vascularity was not observed, however, tumorous lesions and cysts could be identified using two evaluation points: echo intensity level and boundary.

DOI： 10.1007/s11282-022-00605-2

歯科法医学と医療情報招待

柳文修

岡山歯学会雑誌 41 ( 1 ) 1 - 6 2022年6月

担当区分：筆頭著者,　最終著者,　責任著者記述言語：日本語出版者・発行元：岡山歯学会

岡山大学歯学部付属病院における歯科医療情報の、平時の取組と有事の際の医療情報保護について概観した。歯科法医学の重要な役割の一つは個人識別で、大災害時にその有用性が再認識されることが多い。筆者は2014年から個人識別に関する研究を学内共同研究として開始し、1)相同化モデル化理論を応用した歯列石膏模型のデータ化による個人識別法の開発、2)口蓋形態の個人差を利用した新規個人識別法の開発を行っている。これらの研究の概要を述べた。

Collision tumor of small cell carcinoma and squamous cell carcinoma of the maxillary sinus: Case report. 査読国際誌

Irfan Sugianto, Yoshinobu Yanagi, Miki Hisatomi, Shunsuke Okada, Yohei Takeshita, Babatunde Olamide Bamgbose, Junichi Asaumi

Molecular and clinical oncology 16 ( 5 ) 96 - 96 2022年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

A collision tumor refers to the coexistence of two diagnostically distinct tumors in a common anatomic space. Collision tumors are rare in the oral and maxillofacial region. The present study reported on the case of an 82-year-old female with a collision tumor in the maxillary sinus consisting of small cell carcinoma (SmCC) and squamous cell carcinoma (SCC). Computed tomography (CT) imaging revealed a mass in the right maxillary sinus. The lesion exhibited heterogeneous low signal intensity (SI) on T1-weighted imaging (T1WI), high SI on short T1 inversion recovery and heterogeneous solid enhancement on contrast-enhanced T1WI. The histopathology result of a biopsy specimen confirmed SmCC. After the patient received a course of chemoradiotherapy, follow-up CT revealed a residual tumor. In a second surgery, a remaining tumor and histopathology revealed SCC with no evidence of SmCC. The final diagnosis was a collision tumor made up of SCC and SmCC.

DOI： 10.3892/mco.2022.2529

Influence of mesiodens on adjacent teeth and the timing of its safe removal. 査読国際誌

Majd Barham, Shunsuke Okada, Miki Hisatomi, Abdullah Khasawneh, Nouha Tekiki, Yohei Takeshita, Toshiyuki Kawazu, Mariko Fujita, Yoshinobu Yanagi, Junichi Asaumi

Imaging science in dentistry 52 ( 1 ) 67 - 74 2022年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Korean Academy of Oral and Maxillofacial Radiology

Purpose: To focus on the effects of the presence of mesiodens on adjacent teeth and to investigate the timing of its safe removal. Materials and Methods: Cone-beam computed tomography examinations, obtained at Okayama University Hospital over a three-year period, were inspected. Data were recorded including the number of mesiodens; associated abnormalities; and the relationship with neighboring structures. Depending on multiple factors, the risk of developing complications due to early extraction of a mesiodens was divided into three categories: high, medium, and low risk. Results: A total of 5,958 cone-beam computed tomography exams were obtained, 460 patients aged 3-85 years were diagnosed with a total of 568 mesiodens, 382 (67.3%) of which were discovered in young patients (age <10 years), and 333 (87.2%) of these were associated with abnormalities. Regarding the risk categories, 11 (1.9%) were considered to be in the high-risk, five (0.9%) in the medium-risk and 552 (97.2%) in the low-risk categories. Moreover, eight out of 11 high-risk mesiodens were extracted and no post-operative complications have been seen. Conclusion: As the results showed that no postoperative complications were seen in all the extracted cases of high-risk mesiodens, this indicates the possibility of safe extraction at an early age which could reduce related future complications.

DOI： 10.5624/isd.20210218

A Case of Odontogenic Myxoma of the Mandible with Expansion to the Alveolar Crest - Comparison of Imaging Findings and Pathological Findings: A Case Report

Saori Yoshida, Yohei Takeshita, Toshiyuki Kawazu, Tatsushi Matsumura, Junichi Asaumi, Hitoshi Nagatsuka, Yoshinobu Yanagi

OPEN DENTISTRY JOURNAL 16 2022年

担当区分：最終著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：BENTHAM SCIENCE PUBL LTD

Background:An odontogenic myxoma is an intraosseous tumor characterized by stellate and spindle-shaped cells embedded in an abundant myxoid or mucoid extracellular matrix. We herein describe an odontogenic myxoma that expanded not only to the bone marrow but also to the outside of the alveolar bone. Diagnosis of an odontogenic myxoma in a tooth-deficient region by imaging findings alone was difficult because the positional relationship between the tumor and the tooth is unknown. Furthermore, some of these odontogenic myxomas reportedly show rapid growth.Case Report:Here, we present the case of a patient, a 44-year-old man, who had a hard, bone-like swelling on his right mandible molar region and mild paresthesia on his right cheek. An odontogenic myxoma and ameloblastoma were suspected based on the imaging findings; however, pathological examination of the biopsy led to a diagnosis of odontogenic myxoma. Right segmental mandibulectomy was performed, and there was no recurrence observed after surgery.Conclusion:To improve the accuracy of imaging diagnosis, it is important to compare the imaging findings with the pathological findings of the surgical specimen. This comparison in the present case revealed differences in the magnetic resonance imaging signal intensity in regions with different types of cell components.

DOI： 10.2174/18742106-v16-2202140

Web of Science

Dynamic contrast-enhanced MRI as a predictor of programmed death ligand-1 expression in patients with oral squamous cell carcinoma

Nouha Tekiki, Mariko Fujita, Tatsuo Okui, Hotaka Kawai, May Oo, Toshiyuki Kawazu, Miki Hisatomi, Shunsuke Okada, Yohei Takeshita, Majd Barham, Hitoshi Nagatsuka, Yoshinobu Yanagi, Jun-Ichi Asaumi

ONCOLOGY LETTERS 22 ( 5 ) 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPANDIDOS PUBL LTD

Immune checkpoint inhibitors (ICIs) targeting programmed death ligand-1 (PD-L1) are highly promising therapies for oral squamous cell carcinoma (OSCC). The assessment of PD-L1 expression may help predicting the therapeutic effect of ICIs and, thus, benefit patient selection. Contrast index (CI) parameters derived from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) have been proven as efficient to assess microvessel density (MVD) in OSCC. The present study aimed to determine the correlation between DCE-MRI parameters and MVD and between DCE-MRI parameters and PD-L1 expression to determine whether DCE-MRI could be used non-invasively to evaluate PD-L1 expression in patients with OSCC. A total of 21 patients with primary OSCC who had undergone a 3T MRI scan, including DCE-MRI, were included in the present study, and CI curve-derived parameters were examined. The MVD and PD-L1 expression in the surgically resected specimens were analyzed using immunohistochemistry (IHC) staining for CD31 and IHC staining for PD-L1, respectively. The results demonstrated that the expression levels of these markers were correlated with DCE-MRI parameters. PD-L1 expression levels were found to be significantly correlated with the maximum CI (CI-max; P=0.007), peak CI (CI-peak; P=0.007), maximum CI gain (CI-gain; P=0.006) and MVD (P=0.001) values. The mean CI-max, CI-peak, CI-gain and MVD values were significantly higher in tumors with high PD-L1 expression (P<0.05). MVD levels were also significantly correlated with the time of CI-max (T-max; P=0.003) and CI-gain (P=0.037). The mean CI-gain was significantly increased, and the mean T-max was significantly shorter in high MVD tumors (P<0.05 and P<0.01, respectively). In summary, the findings from the present study confirmed the correlation between CI parameters, derived from DCE-MRI, and MVD, and suggested that these parameters may be correlated with PD-L1 expression in OSCC tumor cells.

DOI： 10.3892/ol.2021.13039

Web of Science