Faculty Profiles - NAGATSUKA Hitoshi

MISC - NAGATSUKA Hitoshi

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口腔粘膜扁平上皮癌発生における表皮増殖因子受容体の遺伝子増幅に関する研究

永井教之, 劉桂需, 石割裕三, 辻極秀次, 中野敬介, 長塚仁

岡山歯学会雑誌 2000

Fundamental techniques of specimen preparation for morphological study-light microscopy

Nagai, N, Nagatsuka, H, Takagi, T, Nakano, K

PROCEEDINGS of Asian Science Seminar in Okayama 2000 2000

Site dependence of bone formation induced by rhBMP-2: Comparing rat parietal bone and heterotopic back region

H Nagatsuka, N Nagai, T Sakae, RZ LeGeros

BIOCERAMICS 192-1 511 - 514 2000

Language：English Publisher：TRANS TECH PUBLICATIONS LTD

The purpose of this study was to compare the rate of bone formation and progression induced by Rh-BMP-2/atelocollagen complex between the periosteal and dermis regions in rats. In both cases, mature bone formation was preceded by mesenchymal cell proliferation and immature bone formation. In the periosteal region, bone formation was rapid: tissues were almost fully occupied with bone after 6 weeks of implantation, whereas in the dermis region bone tissue was only 80% occupied even after 24 weeks. Vascularization occurred at the cavities of the implant and appeared to be necessary in the formation of immature bone.

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Detection of mRNA in hard tissue using in situ hybridization

Ishiwari,Y, Nagatsuka, H, Tsujigiwa, H, Nakano, K, Nagai, N

PROCEEDINGS of Asian Science Seminar in Okayama 2000 2000

Immunohistochemistry in hard tissue research

Nagatsuka, H, Ishiwari,Y, Nakano, K, Tsujigiwa, H

PROCEEDINGS of Asian Science Seminar in Okayama 2000 2000

Pheomelanin and Eumelanin of Malignant Melanomas and Melanosis in Oral Mucosa

You-Jin LEE, Noriyuki NAGAOKA, Toshiro SAKAE, Tohru TAKAGI, Hitoshi NAGATSUKA, Yasuo SHINNOU, Kazuteru HIBI, Noriyuki NAGAI, Department of oral Pathology Okayama University Dental School, Department of oral Pathology Okayama University Dental School, Department of Oral Anatomy Nihon University school of Dentistly at Matsudo, Department of oral Pathology Okayama University Dental School, Department of oral Pathology Okayama University Dental School, Department of oral Pathology Okayama University Dental School, Department of oral Pathology Okayama University Dental School

Journal of hard tissue biology = Journal of hard tissue biology 10 ( 2 ) 89 - 95 2000

14. Distribution pattern of αl(IV)- α6(IV)chains of type IV collagen in tooth germ morphogenesis(8th Annual Meeting of the Society of Hard Tissue Biology July 24, 1999 in Asahikawa)

Noriyuki Nagai, Keisuke Nakano, Yuzo Ishiwari, Hitoshi Nagatsuka, Yoshiko Kitamura, Tohru Takagi, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Oral Pathology Okayama University Dental School, Department of Biochemistry Faculty of Dentistry of Tokyo Medical and Dental University

Journal of hard tissue biology = Journal of hard tissue biology 9 ( 1 ) 27 - 28 2000

rhBMP-2による異所性骨形成に及ぼす固定化効果

永井教之, 譚軍, 井上正久, 石割裕三, 長塚仁

歯科基礎医学会雑誌 41 ( 5 ) 504 - 504 1999.8

Long-term observation of heterotopic bone formation induced by rhBMP-2 and IBM composite.

Inoue, M, Tan, J, Tsujigiwa, H, Kitamura, Y, Hibi, K, Lee, Y. J, Kan, S, Nojima, T, Hoh, C, Nagatsuka, H, Nagai, N

Bioceramics 1999

口腔粘膜の扁平上皮癌の癌化過程におけるEpidermal growth factor receptor の免疫組織化学的検討

永井教之, 劉桂需, 長塚仁, 石割裕三, 井上正久, 野島鉄人

岡山歯学会雑誌 1999

46 : A case report of malignant melanoma in situ of the palate

Hibi K., Ishiwari Y., Nagaoka N., Inoue M., Nagatsuka H., Nagai N.

Oral medicine & pathology 3 ( 2 ) 114 - 114 1998.12

The Effect of BMP on the Monocyte-Macrophage-Mesenchymal Cell Interaction in Ectopic Bone Formation

Hong In SHIM, Shin Yoon KIM, Hyun Mo RYOO, Yuzo ISHIWARI, Hitoshi NAGATSUKA, Song HAN, Noriyuki NAGAI, Department of Oral Pathology Kyungpook National University, Department of Orthopedics Kyungpook National University, Department of Oral Biochemistry Kyungpook National University, Department of Oral Pathology Okayama University, Department of Oral Pathology Okayama University, Department of Oral Pathology Okayama University, Department of Oral Pathology Okayama University

Journal of hard tissue biology = Journal of hard tissue biology 7 ( 1 ) 11 - 15 1998.6

4. Gene expression of amelogenin in odontogenic tumors :

Nakano Keisuke, Nagatsuka Hitoshi, Inoue Masahisa, Takagi Tohru, Nagai Noriyuki

Journal of hard tissue biology 7 ( 2 ) 62 - 62 1998

Language：English

The distribtution of amelogenin mRNA was investigated in ameloblastoma and adenomatoid odontogeznic tumor (AOT). Digoxygenin(DIG) labeled RNA probe was prepared from human plexiform type amelobastoma. Sections of rat incisors human ameloblastoma and ACT were hybridized with the above-mentioned probe, and immuno-detection of the hybridized probe was performed with the DIG Ntucleic Acid Detection Kit. In the rat incisor, amelogenin mRNA was detected in the preameloblast and ameloblast. Amelogeuuin mRNA was detected mainly in the high columutar cells of the amelobastoma. Amelogenin mRNA was also detected in many duct-like and solid strtuctture tumor cells of AOT.

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Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306625

27D18 : Tumor of mandibular bone

Tsujigiwa H., Nagatsuka H., Inoue M., Takagi H., Nagai N.

Oral medicine & pathology 2 ( 2 ) 109 - 109 1997.12

Aging and ectopic bone formation induced by partially purified bone morphogenetic protein

Chun-Lin Qin, Murata Masaru, Nagatsuka Hitoshi, Inoue Masahisa, Nagai Noriyuki

Japanese journal of oral biology 39 ( 5 ) 572 - 582 1997.10

Gene Expression of Chondro-Osseous Cells in Heterotopic Bone formation Induced by rhBMP-2

Hitoshi NAGATSUKA, Masahisa INOUE, Takumi AKAGI, Yuzo ISHIWARI, Liu GUIRU, Bingzhen HUANG, Tohru TAKAGI, Mohamed Gomaa Z0UAIR-ATTIA, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Biochemistry School of Dentistry Tokyo Medical and Dental University, Department of Oral Pathology School of Dentistry Okayama University:Department of Oral Pathology Faculty of Dental Medicine Al-Azahar University, Department of Oral Pathology School of Dentistry Okayama University

Journal of hard tissue biology = Journal of hard tissue biology 6 ( 1 ) 10 - 15 1997.6

30D19: Left buccal tumor (Proceedings of the 7th Annual Meeting of The Japanese Society for Oral Pathology)

Murata M., Inoue M., Nagatsuka H., Nagai N.

Oral medicine & pathology 2 ( 1 ) 52 - 52 1997.6

Histopathological and Immunohistochemical Study of Heterotopic Chondro-Osseous Tissue Formation Induced by S-200 BMP

Masahisa INOUE, Chun-Lin QIN, Tetundo NOJIMA, Hitoshi NAGATSUKA, Masaru MURATA, Yukio NOSAKA, Takumi AKAGI, Katsuhiro KURODA, Masaru MABUCHI, Keifuku HOH, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University

Journal of hard tissue biology = Journal of hard tissue biology 5 ( 1 ) 1 - 6 1996.3

Characterization of Bovine extracted BMP and development of Bone Collagen I Carrier

Nagai Noriyuki, Murata Masaru, Tsujigiwa Hidetsugu, Inoue Masahisa, Nagatsuka Hitoshi, Nakano Keisuke, Akagi Takumi, Kinuta Yoshihiro, Kanyama Manabu, Qin Chun-Lin, Zhang Shao-Quan, Konouchi Hironobu, Han Song, Huang Bing-Zhen, Tan Jun, Liu Gui-Ru, Gomah Atia, Hibi kazumitsu

Journal of hard tissue biology 5 ( 2 ) 163 - 173 1996

Language：Japanese

A modofied method was established to extracted BMP from bovine bone. SDS-PAGE and Westen blotting were apllied to analize the components of this partially purified BMP. In addition the amino acid sequence was studied using protein sequencer to analyze the unknown bands. Type I collagen derived from bone was used as a carrier whose properties and advantages were studied. The following conclusions are made from this study. 1. The limitation of molecluar weight in tbe early step of BMP purification procedure improves purification efficiency and stability, which makes the applicadon of BMP in big animalos possible. 2. In this method the purified BMP product also contains histone H3, histone 2B and other unknown proteins as well as BMP-2. 3. The atelocollagen derived from bone has proved to be an useful carrier for BMP. This carrier shows characteristics of self absorption and recalcification when embedded in tissues. 4. The minimun bone induction dose of BMP at each step (G-Ext:500μg, Hep:30μg, S200:50μg) and the optimal bone induction dose(G-Ext:5.0mg, Hep:2.0mg, S200:0.3mg) are determined, which makes the applicalion of BMP in cellular biology possible.

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Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=306598

Immunohistochemical Study of Cytokeratin Patterns in Follicular and Plexiform Ameloblastoma

Hitoshi NAGATSUKA, Hong-In SHIN, Hee-Kyung PARK, Yuzo ISHIWARI, Katsuhiro KURODA, Yukio NOSAKA, Han SONG, Chun-Lin QIN, Shao-Quan ZHANG, Keisuke NAKANO, Yoshiho CHIGONO, Hidetsugu TSUJIGIWA, Tohru TAKAGI, Noriyuki NAGAI, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Kyungpook National University, Department of Oral Pathology School of Dentistry Kyungpook National University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, Department of Oral Pathology School of Dentistry Okayama University, First Department of Oral Surgery Faculty of Medicine Teikyo University, Department of Oral Pathology School of Dentistry Okayama University, Department of Biochemistry Tokyo Medical and Dental University Faculty of Dentistry Yushima, Department of Oral Pathology School of Dentistry Okayama University

Journal of hard tissue biology = Journal of hard tissue biology 4 ( 2 ) 50 - 56 1995.11

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