研究者詳細 - 長塚　仁

論文 - 長塚　仁

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Localization of type IV collagen α-1 to α-6 chains in basement membrane during mouse molar germ development

Nagai, N, Nakano, K, Sado, Y, Naito, I, Gunduz, M, Tsujigiwa, H, Nagatsuka, H, Ninomiya, Y, Siar, C.H

The International Journal of Developmental Biology 45 ( 7 ) 827 - 831 2001年

Human α-and β-defensin immunoreactivity in oral mucoepidermoid carcinomas

N. Mizukawa, K. Sawaki, H. Nagatsuka, M. Kamio, E. Yamachika, J. Fukunaga, T. Ueno, S. Takagi, T. Sugahara

Anticancer Research 21 ( 3 C ) 2171 - 2174 2001年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：INT INST ANTICANCER RESEARCH

The purpose of this study was to demonstrate the immunohistochemical localization and distribution of human α-and β-defensins, peptides with antimicrobial activity, in oral mucoepidermoid carcinoma tissue. Tissue samples were embedded in paraffin and α- and β-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells that constituted the ducts, as well as neutrophils, were positively immunostained with the anti-α-defensin antibody (HNPs). On the other hand, epidermoid cells and intermediate cells were intensely stained with the anti-β-defensin-2 (HBD-2) antibody. Mucous-secreting cells were clearly not immunostained with the anti-HBD-2 antibody. The epithelial hyperplasia region adjacent to the tumor tissues was also positively immunostained with the anti-HBD-2 antibody.

Quantitation of epidermal growth factor receptor gene amplification by competitive polymerase chain reaction in pre-malignant and malignant oral epithelial lesions

Hitoshi Nagatsuka, Yuzo Ishiwari, Hidetsugu Tsujigiwa, Keisuke Nakano, Noriyuki Nagai

Oral Oncology 37 ( 7 ) 599 - 604 2001年

記述言語：英語掲載種別：研究論文（学術雑誌）

Abnormal amplification of epidermal growth factor receptor (EGFR) gene has been reported widely in various human tumors. However, the status of amplification of this gene in the process of carcinogenesis is not clearly defined. We used competitive polymerase chain reaction (PCR) to study whether EGFR gene is amplified and the degree of amplification in pre-malignant and malignant oral epithelial lesions, and also examined the relationship between EGFR gene aberration and the development of squamous cell carcinoma (SCC). Genomic DNA was extracted from paraffin sections of 17 cases of oral epithelial dysplasia (ED), four cases of carcinoma in situ (CIS), and 20 cases of untreated primary SCC. The extracted DNA was subjected to competitive PCR to amplify EGFR gene. Amplification of the EGFR gene was observed in three cases (17%) of ED, one case of CIS and four cases (20%) of SCC. In cases showing EGFR gene amplification, the degree of amplification was low in ED and CIS cases, whereas it was extremely high in SCC cases. These results suggest that amplification of EGFR gene occurs in the relatively early stage of the development of oral SCC. However, a high level of EGFR gene accumulation probably plays an important role in the progression to invasive cancer. © 2001 Elsevier Science Ltd. All rights reserved.

DOI： 10.1016/S1368-8375(01)00051-3

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Effect of Immobilized rhBMP- 2/Collagen on ST2Cell.

Tsujigiwa, H, Nagatsuka, H, Ishiwari, Y, Nakano, K, Viloria, I.L, Palmario, G.B, hay, S.H, Lee, J.I, Termvidchakorn, O, Nagai, N

Journal of Dental Research 2001年

Age and magnetic effects on ectopic bone formation induced by purified bone morphogenetic protein

Noriyuki Nagai, Masahisa Inoue, Yuzo Ishiwari, Hitoshi Nagatsuka, Hidetsugu Tsujigiwa, Keisuke Nakano, Noriyuki Nagaoka

Pathophysiology 7 ( 2 ) 107 - 114 2000年7月

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）

A mixture of heparin-Sepharose-purified bovine bone morphogenetic protein (BMP) and type I atellocollagen was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month-old rats. The implants were removed at 7, 14 and 21 days after implantation. The effects of rat age on ectopic bone formation were evaluated on the explants using H and E staining, morphometric analysis, alkaline phosphatase (ALP) activity and calcium (Ca) content determination, as well as immunohistochemical staining of type IV collagen present in the basement membrane of blood vessels. On day 14 and 21, bone was observed in 4-week and 10-month-old rats but the amount of bone formed in the later was less than in the former. In 18-month-old rats, bone was first found focally in very limited regions of the explants on day 21 and the amount of bone was much less than in 4-week-old rats. At all periods ALP activity was higher in younger rats. On day 7, there were more blood vessels in the explants of 4-week-old rats than in those of 10- or 18-month-old rats. On day 14 and 21, more blood vessels were found in the central regions of the explants in 4-week-old rats than in the same regions in 10- or 18-month-old rats. The findings in the present study indicated that the rate and quantity of ectopic bone formation were reduced, and that the difference in blood vessel distribution might be related to the reduction in ectopic bone formation in aged rats, and suggest that the difference in blood vessel distribution is related to ectopic bone formation. Magnetism can stimulate ectopic bone formation induced by BMP. Copyright (C) 2000 Elsevier Science Ireland Ltd.

DOI： 10.1016/S0928-4680(00)00036-5

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Inductive and conductive bone formation and vascularization by recombinant human BMP-2 and collagen on parietal bone

Nagai N, Nagatsuka H, Sakae T, Hoshino K, Ishiwari Y, Tsujigiwa H, Takagi T, LeGerose R.Z

Sixth World Biomaterials Congress U.S.A 2000年

Florid Cemento-Osseous Dysplasia with Concomitant Simple Bone Cyst

Sasaki, A, Alcalde, R.E, Tsukamoto, G, Nagatsuka, H, Mese, H, Yoshioka, N, Matsumura, T

The Asian Journal of Oral and Maxillofacial Surgery, 2000年

Age effects on ectopic bone formation induced by purified bone morphogenetic protein

Noriyuki Nagai, Chun Lin Qin, Hitoshi Nagatsuka, Masahisa Inoue, Yuzo Ishiwari

International Journal of Oral and Maxillofacial Surgery 28 ( 2 ) 143 - 150 1999年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MUNKSGAARD INT PUBL LTD

A mixture of heparin-Sepharose-purified bovine bone morphogenetic protein and type I atellocollagen was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month-old rats. The implants were removed at 7, 14 and 21 days after implantation. The effects of rat age on ectopic bone formation were evaluated on the explants using haematoxylin-eosin staining, morphometric analysis, alkaline phosphatase activity and calcium content determination, as well as immunohistochemical staining of type IV collagen present in the basement membrane of blood vessels. On day 14 and 21, bone was observed in 4-week and 10-month-old rats, but the amount of bone formed in the latter was less than in the 4-week-old rats. In 18-month-old rats, bone was first found focally in very limited regions of the explants on day 21 and the amount of bone was much less than in 4-week-old rats. At all periods, akaline phosphatase activity was higher in younger rats. On day 7, there were more blood vessels in the explants of 4-week-old rats than in those of 10-month or 18-month-old rats. On day 14 and 21, more blood vessels were found in the central regions of the explants in 4-week-old rats than in the same regions in 10-month or 18-month-old rats. The findings in the present study indicate that the rate and quantity of ectopic bone formation are reduced in aged rats, and suggest that the difference in blood vessel distribution is related to this reduction in ectopic bone formation.

DOI： 10.1016/s0901-5027(99)80208-2

4週および6ヶ月齢ラットにおける粗製BMP異所性骨誘導の組織学的および免疫組織化学的研究

秦春林, 村田勝, 長塚仁, 黒田勝博, 永井教之

日本口腔インプラント学会誌 = Journal of Japanese Society of Oral Implantology 10 ( 3 ) 288 - 295 1997年9月

記述言語：英語出版者・発行元：公益社団法人日本口腔インプラント学会

The mixture of crude BMP and type I collagen as a carrier was implanted in the subcutaneous tissues of 4-week-old and 6-month-old rats. The effect of aging on ectopic bone formation was evaluated after H＆E and type II collagen immunohistochemical staining. On day 7, type II collagen was detected around chondrocytes in 4-week-old rats while in 6-month-old rats, chondrocytes were absent and type II collagen was negative. On day 14, a large number of chondrocytes were observed with strong staining to type II collagen in 4-week old rats but in 6-month-old rats, chondrocytes were hardly seen. On day 21, a large number of trabecular bones with hematopoietic bone marrows were observed in 4-week-old rats but in 6-month-old rats, hematopoietic bone marrow was absent. The absence of chondrocytes on day 7 and their sparsity on day 14 in 6-month-old rats indicates that crude BMP may induce ectopic bone formation mainly through the intramembranous ossification mode in 6-month-old rats but in 4-week-old rats, mainly through endochondral ossification. The results suggested that the differentiation of mesenchymal cells to hard tissue forming cells was delayed and bone formation was quantitatively less in 6-month-old rats than in 4-week-old rats, and that the difference in the ossification mode could be one of the reasons causing the delay and reduction of hard tissue formation in 6-month-old rats.

DOI： 10.11237/jsoi.10.288

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Verruciform xanthoma of the oral mucosa: An immunohistochemical and ultrastructural study of two cases

HI Shin, KS Choi, H Nagatsuka, M Murata, N Nagai

ORAL ONCOLOGY 33 ( 4 ) 279 - 283 1997年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

Verruciform xanthoma is an uncommon benign lesion of unknown aetiology and pathogenesis. Two cases of verruciform xanthoma are presented together with an immunohistochemical and ultrastructural study. One case co-occurred with an odontogenic keratocyst which, to our knowledge, has not been previously reported, and another case occurred on masticatory mucosa with an asymptomatic, granular, exophytic lesion causing a cleft-like gingival recession. In an immunohistochemical study using antibodies to macrophage (CD 68[KP1]), alpha-l-antitrypsin, vimentin, desmin, keratin, neuron-specific enolase (NSE) and S-100 protein, the characteristic foam cells of verruciform xanthoma showed strong positive staining for CD 68[KP1] and vimentin and weak positive staining for alpha-l-antitrypsin, while the other antibodies were negative. As in previous studies, S-100 protein positive dendritic cells were detected in lesional connective tissue. In addition, the ultrastructural findings revealed characteristics of macrophages containing varying sized lipid vacuoles and degenerating epithelial cells. These findings support the concepts that the foam cells in verruciform xanthoma are of monocyte-macrophage lineage and that the epithelial degeneration from uncertain causes may be related to the pathogenesis of the lesion. (C) 1997 Elsevier Science Ltd.

DOI： 10.1016/S0964-1955(96)00080-2

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OSSIFICATION PROCESS-INDUCED BY BMP-CARRIER COMPOSITES

LR MISSANA, N NAGAI, T TAKAGI, M INOUE, H NAGATSUKA

JOURNAL OF DENTAL RESEARCH 74 ( 3 ) 734 - 734 1995年3月

エナメル上皮腫，悪性エナメル上皮腫の増殖細胞核抗原（ＰＣＮＡ）による免疫組織化学的検討

林勝彦, 長塚仁, 井上正久, 永井教之

日本口腔科学会雑誌 44 ( 1 ) 7 - 13 1995年

記述言語：日本語出版者・発行元：Japanese Stomatological Society

Proliferative potential and cell kinetics of ameloblastoma and malignant ameloblastoma were studied by immunohistochemical staining with monoclonal anti-PCNA antibody (PC10. Novocastra). In this study, 18 primary, 6 recurrent and 2 malignant ameloblastomas were used. Anti-PCNA antibody was labelled in proliferating cells in all the cases. In primary ameloblastomas, the labelling indices showed no significant difference between follicular (mean 13.1%) and plexiform type (15.9%) of ameloblastomas. However, the distribution pattern of PCNA-positive cells in follicular ameloblastomas was different from that of plexiform type: In the former, only peripheral tumor cells of tumor parenchyma were positive for and-PCNA, but in the latter, PCNA-positive cells were diffusely distributed in tumor parenchyma. In recurrent ameloblastomas, it was noted that PCNA positive rate was higher (22.2%) than that of primary lesions (13.8%). Malignant ameloblastomas showed that the growth fractions were remarkably higher (43.3%) than primary ameloblastomas. The results of this study clearly show that recurrent ameloblastomas have high proliferating potential in comparison with primary lesions. Moreover, it is suggested that when it is difficult to distinguish between ameloblastomas and malignant ameloblastomas under light microscopy, PCNA immunostaining is useful to differentiate them.

DOI： 10.11277/stomatology1952.44.7

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Immunohistochemical demonstration of tenascin and fibronectin in odontogenic tumours and human fetal tooth germs 査読

N. Nagai, E. Yamachika, K. Nishijima, M. Inoue, H. I. Shin, M. S. Suh, H. Nagatsuka

European Journal of Cancer. Part B: Oral Oncology 30 ( 3 ) 191 - 195 1994年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD

The distribution of tenascin and fibronectin (plasma fibronectin) was studied immunohistochemically in ameloblastomas, ameloblastic fibromas and ameloblastic carcinomas, as well as in tooth germs using monoclonal antibodies. Tenascin is an extracellular matrix molecule that was shown to be enriched in the embryonic mesenchyme surrounding the budding epithelium in various organs, including the tooth. Tenascin was strongly expressed in the basement membrane zone of the ameloblastomas and in the early tooth germ and the dental lamina, but not in the dental follicle. The expression of tenascin in the ameloblastic fibroma was seen in the basement membrane of the epithelial islands throughout the stromal tissues. There were clear differences in fibronectin expression in the follicular ameloblastoma and ameloblastic carcinoma. The results suggest that tenascin and fibronectin are involved in epithelial mesenchymal interactions of the tooth germ and in odontogenic tumours. © 1994.

DOI： 10.1016/0964-1955(94)90090-6

生体代替材料の組織界面の成立と異所性骨誘導蛋白BMPの登場共著査読

永井教之, 長塚仁, 井上正久, 村田勝, 赤木巧, 寒河江登志朗, 高木亨, 水野守道, 滝田裕子, 久保木芳徳

東京都歯科医師会雑誌 42 533-543 1993年9月

ヒト胎児およびラット胎児歯はいのアメロジェニン，ＩＶ型コラーゲン，ファイブロネクチン局在の免疫組織化学的研究

日名雅彦, 井上正久, 長塚仁, 高木享, 永井教之

日本口腔科学会雑誌 42 ( 4 ) 659 - 664 1993年

記述言語：日本語出版者・発行元：Japanese Stomatological Society

In order to examine the distribution of amelogenin, type IV collagen and fibronectin in tooth germs of rat and human fetus, immunohistochemical studies were carried out. The following antibodies were used: polyclenal antibody against bovine amelogenin, monoclonal antibodies against rat and human type IV collagen and polyclonal antibodies against rat and human fibronectin. Specimens were tooth germs of mandit ular first molar of rats at 1st day after birth and mandibular first incisor of human fetus at 16 to 24 weeks. Immunoreactivity of amelogenin was found from the early stage of secretory ameloblast. Also we found the penetration in predentin and intercellular space of odontoblasts. At the inner enamel epithelium of human fetus, tooth germ was found a scanty immunoreactivity of amelogenin, whereas it was not found in rats. Immunoreactivity of type IV collagen was found in the basement membrane of both tooth germs. After the predentin was secreted at the epithelial-mesenchymal interface, uniform immunoreactivity was founded and when the dentin was produced and the epithelial cells were differentiated into ameloblasts, the immunoreactivity was abundant. Immunoreactivity of fibronectin was found in the basement membrane, mesenchymal cells predentin and mineralized dentin. When the immunoreactivity of type IV collagen was abundant and the penetration of amelogenin was found in predentin, an intense immunoreactivity of fibronectin was found in predentin. These results suggest the possibility that the penetration of amelogenin toward the odontoblasts is related to the mineralization of predentin. In conclusion, these phenomenon was observed in rat and human fetnc tooth germs.

DOI： 10.11277/stomatology1952.42.659

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Ameloblastic carcinoma: case report and review

Noriyuki Nagai, Nobuyoshi Takeshita, Hitoshi Nagatsuka, Masahisa Inoue, Katsumi Nishijima, Tetsudo Nojima, Masako Yamasaki, Chofuku Hoh

Journal of Oral Pathology & Medicine 20 ( 9 ) 460 - 463 1991年10月

掲載種別：研究論文（学術雑誌）

The histologic classification for odontogenic carcinomas is still under revision; thus, the differentiation between the terms “malignant ameloblastoma” and “ameloblastic carcinoma” has not been definitely stated. Nevertheless, it is recommended to reserve the former for those lesions that, in spite of an apparently innocuous histology, have given origin to metastatic growths, and to apply the latter for those ameloblastomas in which there is histologic evidence of malignancy in the primary, recurrent or metastatic lesions. A case of an ameloblastic carcinoma in the mandible is presented. Histologically, it was characterized by areas with features of a typical ameloblastoma and areas with anaplastic appearances. Copyright © 1991, Wiley Blackwell. All rights reserved

DOI： 10.1111/j.1600-0714.1991.tb00439.x

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歯原上皮性腫ようの細胞骨格・アメロジェニン・エナメリンの局在に関する免疫組織化学的検討

永井教之, 竹下信義, 長塚仁, 赤木巧, 山崎正子, 方肇福, 野島鉄人, 高木享, 久保木芳徳

口科誌 40 ( 4 ) 746 - 752 1991年10月

記述言語：日本語出版者・発行元：特定非営利活動法人日本口腔科学会

Immunohistochemical expressions of cytokeratin, amelogenin, and enamelin were studied in odontogenic tumors: ameloblastoma, adenomatoid odontogenic tumor and ameloblastic carcinoma. Four anti-cytokeratin monoclonal antibodies were used in this study: CK-1 to total keratin, SE-K to 56, 56.5, 58 and 68 Kd, NSE-K to 52.5 Kd, and 19 K to 40 Kd. Cytokeratin expression in follicular ameloblastoma was similar to that in tooth germ epithelia, and in plexiform ameloblastoma similar to that in fetal oral mucosa. Both types of ameloblastoma also showed slight immunoreactivity for amelogenin. In adenomatoid odontogenic tumor there was a difference between cytokeratin expression in central cells and in peripheral cells in the tumor nests. Moreover, cytokeratin expression in adenomatoid odontogenic tumor was completely different from those in ameloblastoma and tooth germ. Immunoreactivity for amelogenin and enamelin in adenomatoid odontogenic tumor was found in colloidal drops, calcified masses and lining cell membrane of ductlike structure. Therefore, it is possible that these tumor cells differentiate into enamel protein-producing cells, more than those of ameloblastoma do. Immunohistochemical results in ameloblastic carcinoma showed that this carcinoma is composed of undifferentiated odontogenic tumor cells.

DOI： 10.11277/stomatology1952.40.746

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岡山大学歯学部附属病院および口腔病理学講座における６年間の病理組織診断の統計学的検討

永井教之, 竹下信義, 小野俊朗, 辻孝憲, 茅野晃弘, 長塚仁

日本口腔科学会雑誌 38 ( 1 ) 7 - 19 1989年

記述言語：日本語出版者・発行元：Japanese Stomatological Society

In the present study, 2, 807 surgical specimens which were derived from the hospital attached to Okayama University Dental School during a 6 years period from 1982 to 1988 could be analyzed in a variety of ways. Also, 349 biopsy specimens taken in personal dental clinics by dentists during 4 years period from could be analyzed. 2, 807 specimens in the hospital attached to our dental school were composed of 2, 224 specimens (2, 022 patients) for histopathological diagnosis, 52 specimens for frozen-section examination, and 531 specimens for cytological diagnosis. Almost 60% of oral pathologic conditions were represented by cysts, and about 30% were tumors. While inflammatory and cystic lesions composed the largest group of all oral biopsy specimens, the second most common oral lesion was squamous cell carcinoma. About 60% of all oral tumors were benign and about 40% were malignant. Of 230 patients with malignant tumors, the majority were squamous cell carcinoma (184 patients, 80%), followed by adenoid cystic carcinoma, malignant lymphoma, and mucoepidermoid carcinoma. Almost 70% of 349 biopsy specimens that were taken in personal dental clinics by dentists were radicular cysts, and dental granuloma. Although malignant tumors were extremely rare in dental clinics, it appears that the dentists in dental clinics must take a greater role in the total care of the oral cavity.

DOI： 10.11277/stomatology1952.38.7

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