研究者詳細 - 長塚　仁

論文 - 長塚　仁

分割表示 478 件中 401 - 420 件目／全件表示 >>

Expression and localization of cartilage-specific matrix protein chondromodulin-I mRNA in salivary pleomorphic adenomas 国際誌

Kimihide Kusafuka, Keisuke Nakano, Yuji Hiraki, Chisa Shukunami, Hitoshi Nagatsuka, Noriyuki Nagai, Tamiko Takemura, Yutaku Sakaguchi, Kazuichi Okazaki, Michi Kusafuka, Hiroko Hisha, Susumu Ikehara

Virchows Archiv 446 ( 1 ) 34 - 40 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER

Pleomorphic adenoma is the most common epithelial tumor in the salivary glands. This tumor frequently exhibits "mesenchyme"-like components, including myxoid or chondroid areas. Recently, using immunohistochemical techniques, we reported that cartilage-specific matrix protein, chondromodulin-I (ChM-I), was deposited on the inter-territorial matrix of the chondroid area in salivary pleomorphic adenomas and that ChM-I, which is also a strong angio-inhibitory factor, plays an important role in the avascular nature of the chondroid area and the chondroid formation in this type of tumor. To elucidate which cells express ChM-I mRNA in pleomorphic adenomas, we examined the expression and localization of ChM-I mRNA in this type of tumor using an in situ hybridization technique. Immunoreactivity for ChM-I was observed in the inter-territorial matrix of the chondroid area, especially around the lacunae, and in the cytoplasm of neoplastic myoepithelial cells of the myxoid element of pleomorphic adenomas. On in situ hybridization analysis, strong signals for ChM-I mRNA were detected in the cytoplasm of the lacuna cells of the chondroid element, and moderate to marked signals were observed in the cytoplasm of the neoplastic myoepithelial cells of the myxoid element. Signals for ChM-I mRNA were also seen in the cytoplasm of the spindle-shaped neoplastic myoepithelial cells in the transitional areas between the myxoid and chondroid elements of this tumor. Signals for ChM-I mRNA were not seen in the inner ductal cells or the fibrous element. These findings indicate that lacuna cells and neoplastic myoepithelial cells express ChM-I mRNA and that mature ChM-I, which lacuna cells and neoplastic myoepithelial cells translate, is deposited in the chondroid matrix of pleomorphic adenomas. In conclusion, lacuna cells and neoplastic myoepithelial cells express ChM-I mRNA ectopically in pleomorphic adenoma, and this plays an important role in chondroid formation and hypovascularity in this type of tumor. © Springer-Verlag 2004.

DOI： 10.1007/s00428-004-1125-y

Genetic and epigenetic alterations of BRG1 promote oral cancer development. 国際誌

Esra Gunduz, Mehmet Gunduz, Mamoru Ouchida, Hitoshi Nagatsuka, Levent Beder, Hidetsugu Tsujigiwa, Kunihiro Fukushima, Kazunori Nishizaki, Kenji Shimizu, Noriyuki Nagai

International journal of oncology 26 ( 1 ) 201 - 210 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PROFESSOR D A SPANDIDOS

Loss of heterozygosity (LOH) analysis is a sensitive method to detect deletions of specific chromosome regions which are considered to harbor of putative tumor suppressor gene (TSG)s. Previous allelotype analyses in various human cancers suggested the presence of at least one TSG in chromosome 19p13 region. Functional analysis of BRG1, a member of SWI/SNF complex proteins, located at 19p13.2, suggested it to be a candidate TSG in different types of human cancers. We examined LOH at 19p13 region in 39 oral cancers by using six microsatellite markers and found allelic deletion in 25 of 39 (64%) samples. A microsatellite marker near BRG1 locus showed the highest LOH in tumor samples. To clarify the role of BRG1 gene in oral carcinogenesis, we checked its mutation status in tumor samples. As mutation of the BRG1 gene was not detected in oral cancers, we examined the mRNA expression level. Quantitative RT-PCR analysis surprisingly demonstrated increased expression of BRG1 mRNA in 62% of primary tumors as compared with that of matched normal samples. Since functions of BRG1 and BRM, another member of SWI/SNF highly homologous to BRG1, may be interrelated, we also examined BRM mRNA expression in the same samples. In most samples, the expressions of BRG1 and BRM changed in the same direction consistent with reported data on human cancer cell lines. An important finding is that expression of an alternative in frame splicing form of BRG1 which includes exon 26 is selectively decreased or lost in most tumor samples. This unique 33 amino-acid sequence of BRG1 protein shows very high homology with heterogeneous nuclear ribonucleoprotein E and may affect the function and level of BRG1 through modifications on post-transcriptional control. All these findings suggest that the genetic and epigenetic alterations of BRG1 may have a role in oral cancer development.

DOI： 10.3892/ijo.26.1.201

Detecting the (Epidermal Growth Factor Receptor) EGFR Gene Amplification in Oral Carcinogenesis

Katsunori Kanata, Mahmoud AL Sheikh Ali, Hitoshi Nagatsuka, Gui Ru Liu, Masaki Takayama, Hideki Imagami, Noriyuki Nagai, Park Hae Ryoun

Journal of Hard Tissue Biology 14 ( 2 ) 251 - 252 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Abnormal amplification of the epidermal growth factor receptor (EGFR) gene has been reported in various human tumors. We used competitive polymerase chain reaction (PCR) to study weather EGFR gene is amplified and the degree of amplification. We used in this study 17 cases of oral epithelial dysplasia (ED), 4 cases of carcinoma in Situ (CIS) and 20 cases of primary squamous cell carcinoma SCC. The extracted DNA was subjected to competitive PCR to amplify EGFR gene. Amplification of EGFR gene was observed in 3 cases (17%) of ED, 1 case of CIS and 4 cases (20%) of SCC. The degree of amplification was low in ED and CIS but extremely high in SCC. This suggests that amplification of EGFR occurs in early stage of oral SCC however high levels of EGFR plays a role in the progression to invasive carcinoma. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.251

Heparanase mRNA Gene and Protein Expression in Oral Cancer Development and Progression

Hitoshi Nagatsuka, Naoki Katase, Ryo Tamamura, Noriyuki Nagai, Chong Huat Siar, Lee Jae-Il, Hong Sam-Pyo

Journal of Hard Tissue Biology 14 ( 2 ) 243 - 244 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

The human heparanase gene, an endo-beta-D-glucuronidase, has recently been cloned. It functions as an extracellular degradative enzyme that cleaves heparan sulfate proteoglycans, and acts as a critical modulator of tumor metastasis and angiogenesis. Heparanase RNA probe and monoclonal anti-heparanase antibodies were used to examine the expression of heparanase mRNA gene and protein in epithelial dysplasia, carcinoma-in-situ and/or microinvasive carcinoma and oral squamous cell carcinoma. Strong signal and expression of both gene and protein were detected in epithelium progressing from dysplasia to invasive carcinoma. Carcinomatous cells at the tumor invasive front showed the highest levels of heparanase mRNA gene and protein. These results suggest that heparanase plays an important role during oral cancer development and progression, and this may have both prognostic and therapeutic implications. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.243

In Vivo Response of Osteoblast-like and Odontoblast-like Cells in Intraperitoneal Diffusion Chamber

Andrea Paola Rodriguez, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Kazuo Ichikawa, Noriyuki Nagai, Atsuhisa Minonishi, Missana Liliana, C. L. Qin

Journal of Hard Tissue Biology 14 ( 2 ) 294 - 295 2005年1月

掲載種別：研究論文（学術雑誌）

Preliminary analysis of cells before using them in tissue engineering is mandatory. Thus, to clarify the behavior of cells in an appropriate experimental model, we evaluated the characteristic of MDPC-23 cells and KUSA/A1 cells in vitro and in vivo seeded in diffusion chamber. Our results indicated that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber. Whereas, MDPC-23 cells were odontoblast-like cells but with low ability to induce dentin formation. This study suggests that MDPC-23 cells are special cells, which possess morphological and functional character of odontoblast-like cells, expressing DSPP only in vivo with low capacity to induce mineralized dentin matrix. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.294

Human Papillomaviruses Infection in Oral Squamous Cell Carcinoma and Oral Pre-cancer Lesions in Taiwan

Cheng Hsiung Roan, Chin Wan Luo, Chung Ji Liu, Masahisa Inoue, Kojun Setu, Hitoshi Nagatsuka, Noriyuki Nagai

Journal of Hard Tissue Biology 14 ( 2 ) 291 - 293 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Human papilloma viruses (HPV) infection is a significant risk factor for uterine cervical carcinoma. Many previous studies have also demonstrated the presence of HPVs in oral epithelia tissue. However, the role of HPV infection in oral squamous cell carcinoma (OSCC) is still controversy. The present study is to determine the frequency and type of HPV in OSCCs and oral precancer lesions. Methods: DNA samples were collected from 51 OSCCs, 46 oral precancer lesions and 90 normal control specimens by cytobrushings. Nested polymerase chain reaction (PCR), and gene-chip are used to identify multiple HPV types in our samples. Results: The positive rates of overall HPV types (14/46, P=0.0216, OR=2.844, CI=1.186-6.816) and of low-risk types (9/46, P=0.0096, OR=5.529, CI=1.597-19.14) are significantly higher in oral precancer lesions than in control samples. The prevalence of high-risk type (11/51, P=0.0420, OR=2.819, CI=1.051-7.558) is significantly higher in OSCCs than in control but of overall types (13/51, P=0.1066, OR=2.244, CI=0.9266-5.337) is not to reach the statistical significance.Conclusion: The high-risk HPV may play a role in OSCCs progression and the low-risk ones may associate with the oral precancer lesions. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.291

Type I,II and X Collagen Gene Expression Pattern in Bone Formation Using rh-BMP2

Naoki Katase, Hitoshi Nagatsuka, Yuzo Ishiwari, Hironobu Konouchi, Noriyuki Nagai, Masao Inoue

Journal of Hard Tissue Biology 14 ( 2 ) 239 - 240 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Bone morphogenic protein (BMP) induces ectopic bone. In that process, there are some tissue-specific collagens (type I, II and X) in osteocartilagenous matrix. And these types of collagen are also observed in the process of ossification in condylar cartilage. We searched for the localization of collagen gene expression in ectopic bone induction using rh-BMP and condylar cartilage ossification. BMP-type I collagen complex were implanted into the subcutaneous tissue of the dorsal region of ICR mice. Then we made specimens 3days, 1week, and 2weeks after implanting. For condylar cartilage, we pick out condylar region from ICR mice, and made specimens. The probes was made from mouse type I, II and X collagen cDNA segment (kikindly provided by Dr. Ninomiya of Okayama Universy). The process of ectopic bone induction using rh-BMP was the process through the intermediary of rapidly formed cartilagenoid tissue. And there are some difference of type I, II and X collagen gene expressions between the process using rh-BMP and that of condylar cartilage. Those gene expressions imply that the ectopic hard tissue formative cells induced in early stage will take on the characters of osteoblasts and chondrocytes, and that the cells in growing cell layer in cartilagenoid tissue can differentiate into both osteoblasts and chondrocytes. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.239

Immunohistochemical Study of Collagen Type IV Alpha Chains and MMP-2, -9 at the Basement Membrane in Oral Carcinogenesis

Ryo Tamamura, Hitoshi Nagatsuka, Mehmet Gunduz, Ichiro Naitoh, Noriyuki Nagai, Lihong Ge, Yoshikazu Sado, Adusa Yabe

Journal of Hard Tissue Biology 14 ( 2 ) 237 - 238 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Destruction of basement membrane is an important element in invasion of cancer cells. Type IV collagen, the major component of basement membrane, has six distinct a chains. Matrix metalloproteinases (MMPs) are enzymes that resolve extracellular matrix. Oral squamous cell carcinoma is occurred after precancerous lesions, epithelial dysplasia and carcinoma in situ. We investigated localization of six a chains and MMPs in normal oral mucosal tissue, precancerous lesions, early squamous cell carcinoma immunohistochemically. In normal oral mucosal tissue and epithelial dysplasia, α1 (IV), α2 (IV), α5 (IV) and α 6 (IV) chains were detected continuously along basement membrane. In carcinoma in situ and early squamous cell carcinoma, either α5 (IV) and α6 (IV) chains or all a chains were not stained. In contrast, MMP-2 and MMP-9 that are members of type IV collagenase were stained at the parts of disappearance of a chains. This study suggested that the disappearance of a chains is an important element in carcinogenesis of oral squamous cell carcinoma. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.237

Evaluation of Honeycomb Scaffold Combined with KUSA/A1 Cells for Tissue Engineering

Tohru Takagi, Andrea Paola Rodriguez, Silvia Susana Borkosky, Kan Rui, Hu Haillong, Hitoshi Nagatsuka, Noriyuki Nagai, Shinichiro Oida, Miki Komuro

Journal of Hard Tissue Biology 14 ( 2 ) 223 - 224 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Atelocollagen revealed abundant new bone formation. We also determined the immunolocalization of type I collagen, CD34, Osteocalcin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage especially through the proliferation and differentiation process in a confined space. This study can possible enhances existing therapeutic applications. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.223

Gene Expression of Matrix Proteins during Tooth Germ Development in Cbfal Knockout Mice

Kazuo Ichikawa, Sathi Gul San Ara, Hitoshi Nagatsuka, Yoshiko Kitamura, Noriyuki Nagai, Ying Hao Su, Junji Shimono, Toshifumi Komori

Journal of Hard Tissue Biology 14 ( 2 ) 225 - 226 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Cbfa1(Runx2) is a well recognized factor for osteoblast differentiation. However its role during odontogenesis is not well known. We examined the morphogenesis of tooth and gene expression of matrix protein in Cbfa1-knockout mice at ed 17.5 and day 0 of birth and compared them with mandible bone development. Incisor tooth germ showed morphological and functional differentiation of odontoblasts with expression of osteopontin and osteocalcin, whereas the mandible bone-forming site displayed lack of osteoblastic differentiation, and absence of osteopontin and osteocalcin expression. Stage-specific and cytodifferentiation differences demonstrated: incisor tooth germ progressed to the bell stage, whereas molar tooth germ showed maturational arrest at bud to cap stage. Present findings suggest that 1. Cbfa1 is associated with morphogenesis of teeth and matrix protein gene expression, 2. Compared to the incisor tooth germ, the molar tooth germ is more strongly subjected to control by Cbfa1, and 3. In Cbfa1-knockout mice, the odontoblast-like cells in the incisor and the spindle cells in the mandible forming region showed different patterns of gene expression of matrix proteins which are common to both teeth and bones. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.225

Effects of Immobilized Recombinant Human BMP-2/Collagen on Cellular Activity of Osteoblast Like Cells

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Mehmet Gunduz, Masao Yamada, Noriyuki Nagai, Jin Tan, Katsunori Kanata

Journal of Hard Tissue Biology 14 ( 2 ) 126 - 127 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

The use of rhBMP-2 to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization. (2) Studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated in augmenting cellular activity. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.126

Expression of collagen IV α1 to α6 chains in basement membranes of odontogenic tumors.

Nagatsuka H, Siar CH, Fujii E, Tamamura R, Katase N, Morimoto Y, Nagai N

J Hard Tissue Biol 14 ( 2 ) 130 - 131 2005年1月

Localization of type IV collagen alpha chains in tooth germ development

Naoki Katase, Hitoshi Nagatsuka, Keisuke Nakano, Ryo Tamamura, Noriyuki Nagai, Xie Wel-li, Yukiteru Matsuoka

Journal of Hard Tissue Biology 14 ( 2 ) 124 - 125 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

The morphogenesis and morphological development of molar are regulated by the interaction between odontogenic epithelium and mesenchymal tissues. And Type IV collagen molecules, the major component of the dental basement membrane, are composed of three alpha chains. Using indirect immunofluorescence on cryosections, we characterized the changes in protein localization of alpha 1 to 6 chains of type IV collagen during molar development in mice molar and human tooth germ. ICR mouse embryos (detection of vaginal plug: day 0) and newborn mice were used. Human tooth germ was kindly provided by Dr. Yamamoto of Ohu University. Rat monoclonal antibodies recognizing type IV collagen alpha chains were raised against synthetic peptides of non-consensus amino acid sequences of the human and mice alpha (IV) chains. These monoclonal antibodies were kindly provided by Shigei medical research institute. Both mice and human tooth germ, we detected conversions in localization and composition of type IV collagen alpha chains working with tooth germ development. These results imply that there are time and site specificity, which may be involved in tooth morphogenesis and the tooth germ development, in type IV collagen alpha chains. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.124

Angiogenic Squamous Dysplasia in Oral Epithelial Dysplastic Lesions

Chong Huat Siar, Valerie Oo, Hitoshi Nagatsuka, Hidetsugu Tsujigiwa, Mehmet Gunduz, Noriyuki Nagai, Kok Han Ng

Journal of Hard Tissue Biology 14 ( 2 ) 185 - 186 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Statement of problem: Angiogenic squamous dysplasia (ASD), a qualitative distinct form of angiogenesis, was first described in pre-invasive bronchial mucosa of high-risk individuals. It is characterized histologically by the presence of capillary tufts that are closely juxtaposed to and projecting into the dysplastic bronchial epithelium. Objective: To determine whether ASD occurs in oral epithelial dysplastic lesions. Methods: Sixty cases of potentially malignant oral epithelial lesions comprising 20 mild epithelial dysplasia (ED), 20 moderate ED and 20 severe ED(inclusive of carcinoma-in-situ), and 10 normal oral mucosa (as normal controls) were retrieved from the archives of the Department of Oral Pathology, Oral Medicine & Periodontology, Faculty of Dentistry, University of Malaya, and the Cancer Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. The grading of oral ED was in accordance with the recommendations of the World Health Organization. For all selected cases, new 5 micron thick sections were prepared for H&E staining, and for immunohistochemistry with three vascular markers, CD31, CD34 and CD105, with appropriate positive and negative controls. The established criteria for identification of ASD was applied. Results: ASD was identified in parts of severe oral ED, but was absent in normal oral epithelium, mild and moderate oral ED. About 65% of these severe oral ED cases were from individuals with high-risk habits. As with bronchial ASD, the capillaries typically formed CD31- and CD34-positive projections or loops that abut onto the overlying dysplastic oral epithelium causing the latter to assume a papillary surface configuration. Unlike bronchial ASD which occurs in respiratory-type epithelium, oral ASD was found in keratinzed stratified squamous epithelium. CD105 confirmed the presence of neoangiogenesis. Conclusions: Present findings confirm that ASD can occur in oral severe ED. It also demonstrates that this angiogenic abnormality is not unique to bronchial mucosal dysplastic lesions. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.185

Localization of Collagen Type IV Alpha Chains at the Basement Membrane of Oral Squamous Cell Carcinoma

Ryo Tamamura, Hitoshi Nagatsuka, Mehmet Gunduz, Noriyuki Nagai, Hong Gong Liu

Journal of Hard Tissue Biology 14 ( 2 ) 194 - 195 2005年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

Destruction of basement membrane is an important element in invasion of cancer cells. Type IV collagen, the major component of basement membrane has six distinct a chains. In this study, we investigated the localization of six a chains and matrix metalloproteinases (MMPs) in oral squamous cell carcinoma immunohistochemically. In well differentiated squamous cell carcinoma, the localization of a chains showed various patterns.α1 (IV), α2 (IV),α5 (IV) αnd α6 (IV) chains were stained in almost all basement membranes in the central of cancer. Unique staining with only α5 (IV) and α6 (IV) chains was also observed. In the invasive point of cancer, all a chains mainly became negative, but MMP-2 and MMP-9 were stained strongly. In poorly differentiated squamous cell carcinoma, both a chains and MMPs were not stained. Furthermore, we examined the correlation between distribution of a1 chain and invasive pattern of cancer. We found that the disappearance of a chains correlated closely with invasive activity of cancer. © 2005, THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY. All rights reserved.

DOI： 10.2485/jhtb.14.194

A study of eight oral malignant melanoma in adults by WESTOP (Western Society of Teachers of Oral Pathology,1995).

Kawahara K, Lee YJ, Nagatsuka H, Rui K, Auerkari EI, Floresca MCGZ, Kishikawa H, Nagai N

J Hard Tissue Biol 14 ( 2 ) 122 - 123 2005年

Immunolocalization of heparanase in ameloblastoma and other odontogenic tumors.

Han PP, Katase N, Tamamura R, Win SS, Okada H, Nakayama Y, Nagatsuka H

J Hard Tissue Biol 14 ( 2 ) 232 - 233 2005年

Multiple osteolytic lesions of the jaws

Han P P, Nagatsuka H, Tsujigiwa H, Gunduz M, Shinno Y, Rodriguez A P, Riveria R S, Nagai N

Oral Medicine & Pathology 2005年

Effect of magnesium, strontium or fluoride ions on in vitro activities of odontoblast-like cells(MDPC-23).

Inoue M, LeGeros RZ, Inoue M, Rohanizadeh R, Guo L, Davidson R, Kan R, Nagatsuka H, Nagai N

J Hard Tissue Biol 14 ( 1 ) 5 - 12 2005年

記述言語：英語出版者・発行元：硬組織再生生物学会

Magnesium (Mg), strontium (Sr) or fluoride (F) ions have been shown to affect in vitro activities of bone cells and in vivo mineralization. The purpose of this study was to investigate the effect of Mg, Sr and F ions on the activities of odontoblast-like cells (MDPC-23) using an in vitro cell culture model. Mg, Sr or F ions were added to the medium in the following concentrations : Mg-1, 5, 10mM; Sr-0.05, 0.1, 0.3, 1mM; F-0.005, 0.01, 0.05, 0.1mM. Results were observed after 1, 7 and 14 days using Von Kossa staining, scanning and transmission electron microscopy. Preliminary results showed the following : Mg ions demonstrated a positive effect at low concentrations and a negative effect at high concentrations; Sr ions demonstrated a positive dose dependent effect up to a concentration of 0.1mM and a negative effect at higher concentrations; and F ions demonstrated a positive dose-dependent effect up to a concentration of 0.005mM and inhibitory effects at higher concentrations. This preliminary study showed that the positive or negative effects of Mg, Sr or F ions on odontoblastic activities (proliferation and mineralization) depend on their concentration levels in the environment.

DOI： 10.2485/jhtb.14.5

その他リンク： http://search.jamas.or.jp/link/ui/2006294924

Localization of type IV collagen alpha chains in the basement membrane of ameloblastoma, tooth germ and oral mucosa by using indirect immunofluorescence.

Kou H, Borkosky SS, Nagatsuka H, Tamamura R, Nakano K, Nagai N

J Hard Tissue Biol 14 ( 2 ) 235 - 236 2005年

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